Mariano A Garcia-Blanco

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing
    Mariano A Garcia-Blanco
    Department of Molecular Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Invest 112:474-80. 2003
  2. ncbi request reprint Methods for the study of alternative splicing
    Mariano A Garcia-Blanco
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University Medical Center, Durham, NC 27710, USA
    Methods 37:289-91. 2005
  3. ncbi request reprint Making antisense of splicing
    Mariano A Garcia-Blanco
    Duke University Medical Center, Department of Molecular Genetics and Microbiology, Center for RNA Biology, Durham, NC 27710, USA
    Curr Opin Mol Ther 7:476-82. 2005
  4. ncbi request reprint Alternative splicing in disease and therapy
    Mariano A Garcia-Blanco
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Box 3053, Research Drive, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Biotechnol 22:535-46. 2004
  5. pmc A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements
    Andrew P Baraniak
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell Biol 23:9327-37. 2003
  6. pmc Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity
    Sebastian Oltean
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:14116-21. 2006
  7. ncbi request reprint MAZ elements alter transcription elongation and silencing of the fibroblast growth factor receptor 2 exon IIIb
    Nicole D Robson-Dixon
    Departments of Molecular Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:29075-84. 2004
  8. pmc Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6
    Irina Evsyukova
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 19:103-15. 2013
  9. ncbi request reprint Characterization of the intronic splicing silencers flanking FGFR2 exon IIIb
    Eric J Wagner
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:14017-27. 2005
  10. pmc Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency
    Nicholas J Homa
    Department of Molecular Genetics and Microbiology, Center for Virology, Duke University Medical Center
    J Virol 87:12291-301. 2013

Research Grants

  1. CELLULAR COACTIVATORS OF THE HIV 1 PROMOTER
    MARIANO GARCIA BLANCO; Fiscal Year: 2003
  2. Imaging Alternative Splicing During Tumor Progression
    MARIANO GARCIA BLANCO; Fiscal Year: 2006
  3. Connections between mRNA elongation and splicing
    MARIANO GARCIA BLANCO; Fiscal Year: 2007
  4. Integrated instrument system for maintenance and delivery of RNAi libraries
    MARIANO GARCIA BLANCO; Fiscal Year: 2008
  5. Regulation of Alternative Splicing of FGFR2 pre-mRNA
    MARIANO GARCIA BLANCO; Fiscal Year: 2008
  6. RNA-Protein Interactions in Flavivirus Infection
    MARIANO GARCIA BLANCO; Fiscal Year: 2008

Collaborators

Detail Information

Publications61

  1. pmc Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing
    Mariano A Garcia-Blanco
    Department of Molecular Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Invest 112:474-80. 2003
    ..This review examines how spliceosomes can recombine two primary transcripts in trans to reprogram messenger RNAs...
  2. ncbi request reprint Methods for the study of alternative splicing
    Mariano A Garcia-Blanco
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University Medical Center, Durham, NC 27710, USA
    Methods 37:289-91. 2005
    ..Many of these methods have been assembled in this volume to assist a newcomer to the study of alternative splicing and to expand the methodologies of laboratories already in the field...
  3. ncbi request reprint Making antisense of splicing
    Mariano A Garcia-Blanco
    Duke University Medical Center, Department of Molecular Genetics and Microbiology, Center for RNA Biology, Durham, NC 27710, USA
    Curr Opin Mol Ther 7:476-82. 2005
    ..Preliminary, but exciting, results suggest that these reagents could have clinical utility in treating previously intractable conditions...
  4. ncbi request reprint Alternative splicing in disease and therapy
    Mariano A Garcia-Blanco
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Box 3053, Research Drive, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Biotechnol 22:535-46. 2004
    ..Recent experiments have used modified oligonucleotides to inhibit cryptic exons or to activate exons weakened by mutations, suggesting that these reagents could eventually lead to effective therapies...
  5. pmc A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements
    Andrew P Baraniak
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell Biol 23:9327-37. 2003
    ..The downstream sequence is very likely a highly conserved GCAUG element, which we show was required for efficient exon IIIb activation...
  6. pmc Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity
    Sebastian Oltean
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:14116-21. 2006
    ..Our data suggest an unforeseen relationship between epithelial mesenchymal plasticity and malignant fitness...
  7. ncbi request reprint MAZ elements alter transcription elongation and silencing of the fibroblast growth factor receptor 2 exon IIIb
    Nicole D Robson-Dixon
    Departments of Molecular Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:29075-84. 2004
    ..We propose that RNAPII pauses at the MAZ4 elements resulting in a change in the transcription elongation complex that influences alternative splicing decisions downstream...
  8. pmc Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6
    Irina Evsyukova
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 19:103-15. 2013
    ..This may be relevant for both T cell ontogeny and function and development of multiple sclerosis...
  9. ncbi request reprint Characterization of the intronic splicing silencers flanking FGFR2 exon IIIb
    Eric J Wagner
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:14017-27. 2005
    ..Our data defined elements of function within the ISSs flanking exon IIIb and suggested that silencing of this exon is mediated by multiple trans-acting factors...
  10. pmc Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency
    Nicholas J Homa
    Department of Molecular Genetics and Microbiology, Center for Virology, Duke University Medical Center
    J Virol 87:12291-301. 2013
    ..Therefore, characterization of global changes in mRNA isoform usage during EBV infection identifies a new mechanism for the maintenance of latent infection. ..
  11. doi request reprint Identification of the cellular targets of the transcription factor TCERG1 reveals a prevalent role in mRNA processing
    James L Pearson
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 283:7949-61. 2008
    ..These findings provide the strongest support to date for a role of TCERG1 in mRNA processing and are consistent with proposals that TCERG1 couples transcription and processing...
  12. pmc Imaging the alternative silencing of FGFR2 exon IIIb in vivo
    Vivian I Bonano
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, 27710, USA
    RNA 12:2073-9. 2006
    ..This work provides a first glimpse at splicing regulation among different cell types in vivo and promises the drafting of an anatomic map of splicing decisions...
  13. pmc Identification of an intronic splicing enhancer essential for the inclusion of FGFR2 exon IIIc
    Puneet Seth
    Department of Molecular Genetics and Microbiology, and Center for RNA Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 283:10058-67. 2008
    ..This fact, together with the requirement for DISE to be close to the 5'-splice site and the ability of DISE to promote binding of U1 snRNP, suggested that IAS1 and DISE belong to the same class of cis-acting elements...
  14. pmc Quantification of alternatively spliced FGFR2 RNAs using the RNA invasive cleavage assay
    Eric J Wagner
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 9:1552-61. 2003
    ..The data presented here indicate that the RNA invasive cleavage assay is an important addition to the repertoire of techniques available for the study of alternative splicing...
  15. pmc Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing
    Heather B Miller
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 17:665-74. 2011
    ..This may represent a novel motif that Tat-SF1 recognizes during transcription. Together, these findings suggest that Tat-SF1 functions independently in transcription and splicing of cellular genes...
  16. pmc Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice
    Andrew P Baraniak
    Department of Molecular Genetics and Microbiology, Box 3053, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell Biol 26:1209-22. 2006
    ....
  17. pmc Fluorescence-based alternative splicing reporters for the study of epithelial plasticity in vivo
    Jason A Somarelli
    Center for RNA Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 19:116-27. 2013
    ..Moreover, our data suggest that keratinocytes migrate efficiently in the absence of a complete EMT...
  18. pmc SplicerEX: a tool for the automated detection and classification of mRNA changes from conventional and splice-sensitive microarray expression data
    Timothy J Robinson
    Molecular Cancer Biology Program, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 18:1435-45. 2012
    ..SplicerEX is freely available upon request...
  19. pmc Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication
    Stacia L Phillips
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University, Durham, North Carolina, USA Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA
    MBio 7:e01865-15. 2016
    ....
  20. pmc The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase
    Barbara J Natalizio
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:8692-702. 2009
    ..We propose that the efficient coupling of transcription to pre-mRNA processing requires not only the phosphorylated CTD but also other RNAP II specific subunits or associated factors...
  21. ncbi request reprint Alternative splicing: therapeutic target and tool
    Mariano A Garcia-Blanco
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Prog Mol Subcell Biol 44:47-64. 2006
    ..Preliminary, but exciting, results in these areas of investigation suggest that these methods could eventually lead to treatments in heretofore intractable ailments...
  22. pmc Factors affecting reproducibility between genome-scale siRNA-based screens
    Nicholas J Barrows
    Department of Molecular Genetics and Microbiology, Duke NUS Graduate Medical School, Durham, NC, USA
    J Biomol Screen 15:735-47. 2010
    ....
  23. ncbi request reprint In vitro coupled transcription splicing
    Barbara J Natalizio
    Department of Molecular Genetics, Center for RNA Biology, Duke University Medical Center, Durham, NC 27710, USA
    Methods 37:314-22. 2005
    ..We also demonstrate how the system can be used to study exon silencing in vitro. We believe that this system will be a useful tool to study the mechanisms that mediate the coupling of transcription and pre-mRNA processing...
  24. pmc hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc
    David M Mauger
    Box 3053 424 CARL, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
    Mol Cell Biol 28:5403-19. 2008
    ..These results establish hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance their ability to antagonize ASF/SF2...
  25. pmc Development of a method to isolate circulating tumor cells using mesenchymal-based capture
    Rhonda L Bitting
    Division of Medical Oncology, Duke Cancer Institute, Duke University, Durham, NC, United States Department of Medicine, Duke University, Durham, NC, United States Center for RNA Biology, Duke University, Durham, NC, United States
    Methods 64:129-36. 2013
    ..This method may complement existing epithelial-based methods and may be particularly useful in patients with bone metastases. ..
  26. pmc G protein-coupled receptor kinase 2 promotes flaviviridae entry and replication
    Caroline Le Sommer
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS Negl Trop Dis 6:e1820. 2012
    ..Together, our findings identified GRK2 as a novel regulator of flavivirus infection and suggest that inhibition of GRK2 function may constitute a new approach for treatment of flavivirus associated diseases...
  27. pmc SplicerAV: a tool for mining microarray expression data for changes in RNA processing
    Timothy J Robinson
    Molecular Cancer Biology Program, Duke University Medical Center, Durham, USA
    BMC Bioinformatics 11:108. 2010
    ..We explored the possibility of using HG-U133 microarray data to identify changes in alternative mRNA processing in several available archival datasets...
  28. pmc 5' exon replacement and repair by spliceosome-mediated RNA trans-splicing
    S Gary Mansfield
    Intronn, Inc, Gaithersburg, Maryland 20878, USA Department of Medicine, Duke University Medical Center, Durham, North Carolina 27713, USA
    RNA 9:1290-7. 2003
    ..These studies demonstrate that (1) SMaRT can be used to reprogram the 5' end of mRNA, and (2) efficiency can be improved substantially...
  29. pmc Of urchins and men: evolution of an alternative splicing unit in fibroblast growth factor receptor genes
    Neville Mistry
    Department of Molecular Genetics and Microbiology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    RNA 9:209-17. 2003
    ..The remarkable conservation of intronic control elements, both in structure and function, indicates that the mechanisms that regulate this alternative splicing unit evolved over 600 million years ago...
  30. ncbi request reprint Interleukin 7 receptor alpha chain (IL7R) shows allelic and functional association with multiple sclerosis
    Simon G Gregory
    Center for Human Genetics, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Genet 39:1083-91. 2007
    ..The SNP influences the amount of soluble and membrane-bound isoforms of the protein by putatively disrupting an exonic splicing silencer...
  31. pmc Snail promotes resistance to enzalutamide through regulation of androgen receptor activity in prostate cancer
    Kathryn E Ware
    Department of Medicine, Division of Medical Oncology, Duke University Medical Center, Durham, NC, USA
    Oncotarget 7:50507-50521. 2016
    ..These findings imply that increased Snail expression during progression to metastatic disease may prime cells for resistance to AR-targeted therapies by promoting AR activity in prostate cancer...
  32. ncbi request reprint A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
    Vivian I Bonano
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Protoc 2:2166-81. 2007
    ..5 years. Fluorescence reporters can be used to image many splicing decisions in normal tissues and organs and can be extended to the study of disease states...
  33. pmc Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs
    Heather B Miller
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 4:e5710. 2009
    ..At the onset of this work, the prevailing hypothesis was that Tat-SF1 was a required cofactor for the viral regulatory protein, Tat; however, this had not previously been formally tested in vivo...
  34. pmc The role of epithelial plasticity in prostate cancer dissemination and treatment resistance
    Rhonda L Bitting
    Division of Medical Oncology, Duke Cancer Institute, Duke University, DUMC Box 102002, Durham, NC, 27710, USA
    Cancer Metastasis Rev 33:441-68. 2014
    ....
  35. pmc Alternative splicing in multiple sclerosis and other autoimmune diseases
    Irina Evsyukova
    Department of Biochemistry, Duke University Medical Center, Durham, NC USA
    RNA Biol 7:462-73. 2010
    ..We summarize recent findings connecting splicing and autoimmune disease, and attempt to find common patterns of splicing regulation that may advance our understanding of autoimmune diseases and open new avenues for therapy...
  36. pmc Dunning rat prostate adenocarcinomas and alternative splicing reporters: powerful tools to study epithelial plasticity in prostate tumors in vivo
    Sebastian Oltean
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Research Drive, Box 3053 424 CARL, Durham, NC 27710, USA
    Clin Exp Metastasis 25:611-9. 2008
    ....
  37. ncbi request reprint A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo
    Lars H Lund
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3053 424 CARL, Research Drive, Durham, NC 27710, USA
    J Gen Virol 84:603-6. 2003
    ..These data support previously proposed, but unproven, molecular models...
  38. pmc Functional genomics approach for the identification of human host factors supporting dengue viral propagation
    Nicholas J Barrows
    Program in Cell and Molecular Biology, Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University Medical Center, Durham, NC, USA
    Methods Mol Biol 1138:285-99. 2014
    ..The protocol herein describes the materials and the procedures necessary to screen a human cell line in order to identify genes which are either necessary for or restrict DENV propagation at any stage in the viral life cycle...
  39. doi request reprint Shedding UV light on alternative splicing
    Matthew S Marengo
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Cell 137:600-2. 2009
    ....
  40. pmc Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers
    Andrew J Armstrong
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cancer Res 9:997-1007. 2011
    ....
  41. pmc Using circulating tumor cells to inform on prostate cancer biology and clinical utility
    Jing Li
    a Duke Cancer Institute, Duke University Medical Center, Durham, NC, USA
    Crit Rev Clin Lab Sci 52:191-210. 2015
    ..Here, we review technologies used to detect and characterize CTCs, and the potential biological and clinical utility of CTC molecular profiling in men with metastatic prostate cancer. ..
  42. doi request reprint Carcinosarcomas: tumors in transition?
    Jason A Somarelli
    Center for RNA Biology, and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA
    Histol Histopathol 30:673-87. 2015
    ..The present review will discuss both the histological and molecular biological evidence of the involvement of epithelial plasticity in driving the mixed phenotypes observed in carcinosarcomas. ..
  43. pmc The polypyrimidine tract binding protein is required for efficient picornavirus gene expression and propagation
    Paola M Florez
    Duke University Medical Center, Box 3020 451 Jones, Research Drive, Durham, NC 27710, USA
    J Virol 79:6172-9. 2005
    ..These data indicate a critical role for the polypyrimidine tract binding protein in picornavirus gene expression and strongly suggest a requirement for efficient IRES-dependent translation...
  44. pmc Cellular migration and invasion uncoupled: increased migration is not an inexorable consequence of epithelial-to-mesenchymal transition
    Daneen Schaeffer
    Center for RNA Biology, Duke University Medical Center, Durham, North Carolina, USA Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA
    Mol Cell Biol 34:3486-99. 2014
    ..Our study demonstrates that enhanced migration is not a phenotypic requirement of EMT, and migration and invasion can be uncoupled during carcinoma-associated EMT. ..
  45. pmc Discovery of insect and human dengue virus host factors
    October M Sessions
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 458:1047-50. 2009
    ..This indicates notable conservation of required factors between dipteran and human hosts. This work suggests new approaches to control infection in the insect vector and the mammalian host...
  46. doi request reprint TIA nuclear proteins regulate the alternate splicing of lysyl hydroxylase 2
    Heather N Yeowell
    Division of Dermatology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Invest Dermatol 129:1402-11. 2009
    ..Identification of these TIA regulatory factors therefore suggests a tool to manipulate cellular LH2 levels in scleroderma so that potential intervention therapies may be identified...
  47. pmc RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation
    Rafael K Campos
    Department of Molecular Genetics and Microbiology, Center for RNA Biology, Duke University, Durham, NC, USA
    J Virol . 2016
    ..We postulate that these ribosomal proteins are required for efficient translation elongation through the viral open reading frame. In summary, this work identifies RPLP1/2 as critical flaviviral host factors required for translation...
  48. pmc Mesenchymal-Epithelial Transition in Sarcomas Is Controlled by the Combinatorial Expression of MicroRNA 200s and GRHL2
    Jason A Somarelli
    Duke Cancer Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA
    Mol Cell Biol 36:2503-13. 2016
    ..Together, our results suggest that a miR-200, ZEB1, GRHL2 gene regulatory network may drive sarcoma cells to a more epithelial-like state and that this likely has prognostic relevance. ..
  49. pmc A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection
    Nicholas J Barrows
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, 301 University Blvd, Galveston, TX 77555, USA Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA
    Cell Host Microbe 20:259-70. 2016
    ..Several drugs reduced ZIKV infection across multiple cell types. This study identifies drugs that could be tested in clinical studies of ZIKV infection and provides a resource of small molecules to study ZIKV pathogenesis. ..
  50. pmc Antisense-mediated affinity purification of dengue virus ribonucleoprotein complexes from infected cells
    Stacia L Phillips
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1055, United States Department of Microbiology and Molecular Genetics, Center for RNA Biology and Department of Medicine, Duke University, 213 Research Drive, Durham, NC 27710, United States
    Methods 91:13-9. 2015
    ..RNA-binding proteins that specifically co-purify with viral RNA using this method can be identified en masse by mass spectrometry. This strategy can potentially be adapted to the purification of any viral RNA species of interest...
  51. pmc Biologic and clinical significance of androgen receptor variants in castration resistant prostate cancer
    Kathryn E Ware
    Departments of Molecular Genetics and MedicineDuke University, 213 Research Dr, 0045 CARL Building, Durham, North Carolina 27710, USADepartment of MedicineDuke Cancer Institute, Duke University, Durham, North Carolina, USAMasonic Cancer CenterUniversity of Minnesota Masonic Cancer Center, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USADepartment of Laboratory Medicine and PathologyUniversity of Minnesota, Minneapolis, Minnesota, USA
    Endocr Relat Cancer 21:T87-T103. 2014
    ....
  52. pmc Assessing incomplete deprotection of microarray oligonucleotides in situ
    Holly K Dressman
    Center for Genome Technology, Institute for Genome Science and Policy, Duke University, Durham, NC, USA
    Nucleic Acids Res 34:e131. 2006
    ..Screening of microarrays with these monoclonal antibodies should guide the consideration given to data derived from these and should enhance the accuracy of the results obtained...
  53. ncbi request reprint RNAi-mediated PTB depletion leads to enhanced exon definition
    Eric J Wagner
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell 10:943-9. 2002
    ..Depletion of endogenous PTB using RNAi increases exon IIIb inclusion in transcripts derived from minigenes and from the endogenous FGF-R2 gene. These data demonstrate that PTB is a negative regulator of exon definition in vivo...
  54. pmc Reprogramming of tau alternative splicing by spliceosome-mediated RNA trans-splicing: implications for tauopathies
    Teresa Rodriguez-Martin
    Medical Research Council Centre for Neurodegeneration, Institute of Psychiatry, King s College London, De Crespigny Park, London SE5 8AF, United Kingdom
    Proc Natl Acad Sci U S A 102:15659-64. 2005
    ..Our results demonstrate that an alternatively spliced exon can be replaced by trans-splicing and open the way to novel therapeutic applications of SMaRT for tauopathies and other disorders linked to aberrant alternative splicing...
  55. ncbi request reprint Imaging alternative splicing in living cells
    Eric J Wagner
    Department of Biochemistry andBiophysics, University of North Carolina, Chapel Hill, USA
    Methods Mol Biol 257:29-46. 2004
    ..In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments...
  56. ncbi request reprint Phenotype correction of hemophilia A mice by spliceosome-mediated RNA trans-splicing
    Hengjun Chao
    Department of Medicine, Mt Sinai School of Medicine, New York, New York 10029, USA
    Nat Med 9:1015-9. 2003
    ..This is the first description of phenotypic correction of a genetic defect by RNA repair in a knockout animal model. Our results indicate the feasibility of using SMaRT to repair RNA for the treatment of genetic diseases...
  57. ncbi request reprint Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing
    Xiaoming Liu
    Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Nat Biotechnol 20:47-52. 2002
    ..These results provide functional evidence for SMaRT-mediated repair of mutant endogenous CFTR mRNA in intact polarized CF airway epithelial models...
  58. pmc Fas-activated serine/threonine phosphoprotein (FAST) is a regulator of alternative splicing
    Maria Simarro
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:11370-5. 2007
    ..Mutational analysis reveals that FAST-mediated alternative splicing is separable from the survival effects of FAST. Our data reveal that nuclear FAST can regulate the splicing of FGFR2 transcripts...
  59. pmc Human transcription elongation factor CA150 localizes to splicing factor-rich nuclear speckles and assembles transcription and splicing components into complexes through its amino and carboxyl regions
    Miguel Sánchez-Alvarez
    Department of Molecular Biology, Instituto de Parasitología y Biomedicine, Parque Tecnológico de Ciencias de la Salud, Avenida del Conocimiento s n, Armilla, 18100 Granada, Spain
    Mol Cell Biol 26:4998-5014. 2006
    ..Our results suggest that sequences located at both the amino and carboxyl regions of CA150 are required to assemble transcription/splicing complexes, which may be involved in the coupling of those processes...
  60. ncbi request reprint Autoregulation of polypyrimidine tract binding protein by alternative splicing leading to nonsense-mediated decay
    Matthew C Wollerton
    Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    Mol Cell 13:91-100. 2004
    ....
  61. ncbi request reprint Reversible cross-linking combined with immunoprecipitation to study RNA-protein interactions in vivo
    Somashe Niranjanakumari
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    Methods 26:182-90. 2002
    ..The results indicate that the RIP assay is a powerful tool to identify RNA-protein interactions in vivo and has the potential to unravel the cellular network of RNP complexes in their native setting...

Research Grants6

  1. CELLULAR COACTIVATORS OF THE HIV 1 PROMOTER
    MARIANO GARCIA BLANCO; Fiscal Year: 2003
    ..Better understanding of HIV-1 gene expression should enhance the possibility to develop novel therapies for AIDS and FIN-i infection. ..
  2. Imaging Alternative Splicing During Tumor Progression
    MARIANO GARCIA BLANCO; Fiscal Year: 2006
    ..We will employ the aforementioned imaging reporters to study the regulation of alternative splicing in living animals. We hope to provide an anatomic map of gene expression based on alternative splicing regulation. ..
  3. Connections between mRNA elongation and splicing
    MARIANO GARCIA BLANCO; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  4. Integrated instrument system for maintenance and delivery of RNAi libraries
    MARIANO GARCIA BLANCO; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  5. Regulation of Alternative Splicing of FGFR2 pre-mRNA
    MARIANO GARCIA BLANCO; Fiscal Year: 2008
    ..We will identify the trans-acting factors required for this inclusion using conventional biochemical methods and a new screen using siRNA libraries and fluorescence-based reporters of alternative splicing. ..
  6. RNA-Protein Interactions in Flavivirus Infection
    MARIANO GARCIA BLANCO; Fiscal Year: 2008
    ..We hope this work will lead to the development of new antiviral therapies. [unreadable] [unreadable] [unreadable]..