Daniel J Lew

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 156:315-26. 2002
  2. ncbi request reprint The spindle assembly and spindle position checkpoints
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Genet 37:251-82. 2003
  3. ncbi request reprint The morphogenesis checkpoint: how yeast cells watch their figures
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Cell Biol 15:648-53. 2003
  4. ncbi request reprint Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae
    D J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, 27710, USA
    Curr Opin Genet Dev 10:47-53. 2000
  5. ncbi request reprint Yeast polarity: negative feedback shifts the focus
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Biol 15:R994-6. 2005
  6. pmc The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p
    J N McMillan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 19:6929-39. 1999
  7. pmc Molecular dissection of the checkpoint kinase Hsl1p
    John Crutchley
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 20:1926-36. 2009
  8. pmc The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation
    Lee Szkotnicki
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:4675-86. 2008
  9. pmc Nucleocytoplasmic trafficking of G2/M regulators in yeast
    Mignon A Keaton
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:4006-18. 2008
  10. ncbi request reprint Interplay between septin organization, cell cycle and cell shape in yeast
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Sci 118:1617-28. 2005

Collaborators

Detail Information

Publications56

  1. pmc Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 156:315-26. 2002
    ....
  2. ncbi request reprint The spindle assembly and spindle position checkpoints
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Genet 37:251-82. 2003
    ..We review the historical origins of these checkpoints and recent progress in understanding their surveillance pathways. We also highlight fascinating but as yet unresolved questions, and examine crosstalk between the checkpoints...
  3. ncbi request reprint The morphogenesis checkpoint: how yeast cells watch their figures
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Cell Biol 15:648-53. 2003
    ..The evidence for these proposals is reviewed, highlighting recent findings indicating that Swe1p degradation is controlled by the cell shape change that accompanies bud emergence...
  4. ncbi request reprint Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae
    D J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, 27710, USA
    Curr Opin Genet Dev 10:47-53. 2000
    ..Thus, specific cell-cycle regulators are well-placed to monitor whether a cell has formed a bud and whether a daughter nucleus has been delivered accurately to the bud following mitosis...
  5. ncbi request reprint Yeast polarity: negative feedback shifts the focus
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Biol 15:R994-6. 2005
    ..A new study of Cdc42p polarization in yeast suggests that the actin cytoskeleton can destabilize the polarity axis, causing Cdc42p foci to wander aimlessly around the cell cortex...
  6. pmc The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p
    J N McMillan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 19:6929-39. 1999
    ..These findings suggest that Hsl1p and Hsl7p interact directly with Swe1p to promote its recognition by the ubiquitination complex, leading ultimately to its destruction...
  7. pmc Molecular dissection of the checkpoint kinase Hsl1p
    John Crutchley
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 20:1926-36. 2009
    ..We suggest that Hsl1p responds to alterations in septin organization, which themselves occur in response to the local geometry of the cell cortex...
  8. pmc The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation
    Lee Szkotnicki
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:4675-86. 2008
    ..We identified elm1 mutants in a screen for defects in Swe1p degradation and show that a phosphomimic T273E mutation in HSL1 bypasses the need for Elm1p in this pathway...
  9. pmc Nucleocytoplasmic trafficking of G2/M regulators in yeast
    Mignon A Keaton
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:4006-18. 2008
    ..Because Swe1p degradation is regulated by cytoskeletal stress, shuttling of Swe1p between nucleus and cytoplasm serves to couple cytoplasmic stress to nuclear cyclin/CDK inhibition...
  10. ncbi request reprint Interplay between septin organization, cell cycle and cell shape in yeast
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Sci 118:1617-28. 2005
    ....
  11. pmc A monitor for bud emergence in the yeast morphogenesis checkpoint
    Chandra L Theesfeld
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 14:3280-91. 2003
    ..We discuss models for translating cellular geometry (in this case, the emergence of a bud) into biochemical signals regulating cell proliferation...
  12. pmc Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex
    Lukasz Kozubowski
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 18:1719-26. 2008
    ..Cdc42p concentrates at a random cortical site during symmetry breaking in a manner that requires the scaffold protein Bem1p. The mechanism whereby Bem1p promotes this polarization was unknown...
  13. pmc Feedback control of Swe1p degradation in the yeast morphogenesis checkpoint
    Kindra King
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 24:914-22. 2013
    ..They also highlight the difficulty in disentangling direct checkpoint pathways from the effects of positive-feedback loops active at the G2/M transition...
  14. pmc Differential susceptibility of yeast S and M phase CDK complexes to inhibitory tyrosine phosphorylation
    Mignon A Keaton
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 17:1181-9. 2007
    ....
  15. pmc Determinants of Swe1p degradation in Saccharomyces cerevisiae
    John N McMillan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 13:3560-75. 2002
    ..The other region did not appear to affect interactions with known Swe1p regulators, suggesting that other as-yet-unknown regulators exist...
  16. pmc Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p
    Hsin Chen
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 23:3814-26. 2012
    ..We suggest that an indirect mechanism linking Rho GTPases and formins via Rho effectors may provide finer spatiotemporal control for the formin-nucleated actin cytoskeleton...
  17. pmc The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint
    Aron R Marquitz
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 21:4012-25. 2002
    ..Surprisingly, Swe1p accumulation occurred normally in bem2 cells, but they were nevertheless unable to promote Cdc28p phosphorylation. Therefore, Bem2p defines a novel pathway in the morphogenesis checkpoint...
  18. ncbi request reprint Scaffold-mediated symmetry breaking by Cdc42p
    Javier E Irazoqui
    Department of Pharmacology and Cancer Biology Duke University Medical Center, Durham, NC 27710, USA
    Nat Cell Biol 5:1062-70. 2003
    ..Moreover, polarization was dependent on GTP hydrolysis by Cdc42p, suggesting that assembly of a polarization site involves cycling of Cdc42p between GTP- and GDP-bound forms, rather than functioning as a simple on/off switch...
  19. pmc Opposing roles for actin in Cdc42p polarization
    Javier E Irazoqui
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 16:1296-304. 2005
    ..In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site...
  20. pmc Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit
    Chun Chen Kuo
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 24:753-9. 2014
    ..These findings reveal a mechanism for negative feedback and suggest that the function of negative feedback via GEF inhibition is to buffer the level of Cdc42 at the polarity site...
  21. pmc Tracking shallow chemical gradients by actin-driven wandering of the polarization site
    Jayme M Dyer
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 23:32-41. 2013
    ..How nonmotile cells might filter out noise is unknown...
  22. pmc Singularity in polarization: rewiring yeast cells to make two buds
    Audrey S Howell
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Cell 139:731-43. 2009
    ..Slowing competition in normal cells also allowed occasional formation of two buds, suggesting that singularity is enforced by rapid competition between Cdc42 clusters...
  23. ncbi request reprint Swe1p responds to cytoskeletal perturbation, not bud size, in S. cerevisiae
    John J McNulty
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Biol 15:2190-8. 2005
    ..Insufficient bud size would cause G2 arrest enforced by the mitotic inhibitor Swe1p, explaining previous findings that some perturbations that block bud growth also trigger Swe1p-dependent cell-cycle arrest...
  24. pmc Genetic interactions among regulators of septin organization
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Eukaryot Cell 3:847-54. 2004
    ....
  25. pmc Interaction between bud-site selection and polarity-establishment machineries in budding yeast
    Chi Fang Wu
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Philos Trans R Soc Lond B Biol Sci 368:20130006. 2013
    ..Instead, we suggest that polarity factors recruit Rsr1, effectively sequestering it from other locations and thereby terminating landmark activity. ..
  26. pmc Negative feedback enhances robustness in the yeast polarity establishment circuit
    Audrey S Howell
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Cell 149:322-33. 2012
    ..These predictions are confirmed experimentally...
  27. ncbi request reprint Eavesdropping on the cytoskeleton: progress and controversy in the yeast morphogenesis checkpoint
    Mignon A Keaton
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Microbiol 9:540-6. 2006
    ..However, the means by which Swe1p responds to actin perturbations once a bud has formed remains controversial...
  28. ncbi request reprint Stress-specific activation mechanisms for the "cell integrity" MAPK pathway
    Jacob C Harrison
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:2616-22. 2004
    ..These findings suggest that stresses provide "lateral" inputs into this regulatory pathway, rather than operating in a linear "top-down" manner...
  29. pmc A morphogenesis checkpoint monitors the actin cytoskeleton in yeast
    J N McMillan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Biol 142:1487-99. 1998
    ..However, the ability to respond to such perturbations by delaying cell cycle progression was restricted to a narrow window of the cell cycle, delimited by the periodic accumulation of the checkpoint effector, Swe1p...
  30. pmc Role of competition between polarity sites in establishing a unique front
    Chi Fang Wu
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, United States
    elife 4:. 2015
    ..By manipulating polarity protein dynamics, we show that resolution of multi-cluster intermediates occurs through a greedy competition between clusters to recruit and retain polarity proteins from a shared intracellular pool. ..
  31. pmc Mechanistic mathematical model of polarity in yeast
    Natasha S Savage
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 23:1998-2013. 2012
    ..These findings indicate that vesicle-mediated delivery of Cdc42p is not required to explain the observed Cdc42p dynamics, and raise the question of whether such Cdc42p traffic actually contributes to polarity establishment...
  32. pmc Modeling vesicle traffic reveals unexpected consequences for Cdc42p-mediated polarity establishment
    Anita T Layton
    Department of Mathematics, Duke University, Durham, NC 27708, USA
    Curr Biol 21:184-94. 2011
    ....
  33. ncbi request reprint Cdc42p, GTP hydrolysis, and the cell's sense of direction
    Javier E Irazoqui
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell Cycle 3:861-4. 2004
    ..We suggest that such cycling is critical for a little-studied "function" of Cdc42p: its ability to designate a unique portion of the cell cortex to become the polarization site, and to become concentrated at that site...
  34. ncbi request reprint A role for the Pkc1p/Mpk1p kinase cascade in the morphogenesis checkpoint
    J C Harrison
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Cell Biol 3:417-20. 2001
    ..The G2 delay involves stabilization of Swe1p in response to various actin perturbations, although this alone is insufficient to produce a long G2 delay...
  35. pmc Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1
    Hui Kang
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710
    Mol Biol Cell 27:1764-75. 2016
    ..Moreover, recruitment of Elm1 is responsive to bud emergence. Our findings suggest that Elm1 plays a key role in sensing bud emergence. ..
  36. pmc Beyond symmetry-breaking: competition and negative feedback in GTPase regulation
    Chi Fang Wu
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Trends Cell Biol 23:476-83. 2013
    ..Negative feedback may prevent polarity clusters from spreading too far, regulate the balance between competition and coexistence, and provide directional flexibility for cells tracking gradients. ..
  37. pmc Symmetry breaking and the establishment of cell polarity in budding yeast
    Jayme M Johnson
    Duke University Medical Center, Durham, NC, USA
    Curr Opin Genet Dev 21:740-6. 2011
    ..Here we review recent work on the nature of the autocatalytic process in budding yeast and on the question of why polarized cells only develop a single 'front'...
  38. pmc Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast
    R A Sia
    Department of Molecular Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 7:1657-66. 1996
    ..We propose that the checkpoint delays nuclear division through post-translational regulation of Swe1 and that transcriptional feedback loops enhance the efficacy of the checkpoint...
  39. ncbi request reprint Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p
    I Bose
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 276:7176-86. 2001
    ..We suggest that Bem1p acts to concentrate polarity establishment proteins at a discrete site, facilitating polarization and promoting Cdc24p phosphorylation at specific times during the cell cycle...
  40. doi request reprint Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast
    Benjamin Woods
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 26:2114-26. 2016
    ..We suggest that GAP activity cooperates with the GDI to counteract the dissipative effect of a previously unappreciated pathway whereby GTP-Cdc42 escapes from the polarity site through the cytoplasm. ..
  41. pmc Polarity establishment requires localized activation of Cdc42
    Benjamin Woods
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710
    J Cell Biol 211:19-26. 2015
    ..Here we show that Cdc42 activation must be localized for successful polarity establishment, supporting local activation rather than local delivery as the dominant mechanism in this system...
  42. pmc Morphogenesis and the cell cycle
    Audrey S Howell
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genetics 190:51-77. 2012
    ..Despite considerable progress, however, many of the most puzzling mysteries in this field remain to be resolved...
  43. ncbi request reprint Polarity establishment in yeast
    Javier E Irazoqui
    Department of Pharmacology and Cancer Biology, Duke University Medical Centre, Durham, NC 27710, USA
    J Cell Sci 117:2169-71. 2004
  44. ncbi request reprint Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control
    D J Lew
    Department of Molecular Cancer Biology, Box 3686, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Cell Biol 8:795-804. 1996
    ..Recent studies have uncovered new and unexpected potential roles, and prompted re-examination of previously assumed roles, of Cdk phosphorylation...
  45. doi request reprint Imaging Polarization in Budding Yeast
    Allison W McClure
    Department of Pharmacology and Cancer Biology, Duke University School of Medicine, C359 LSRC, Box 3813, Durham, NC, 27710, USA
    Methods Mol Biol 1407:13-23. 2016
    ....
  46. pmc Role of Polarized G Protein Signaling in Tracking Pheromone Gradients
    Allison W McClure
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Dev Cell 35:471-82. 2015
    ..Our findings explain why G protein polarization is beneficial and illuminate a novel mechanism for gradient tracking...
  47. pmc Dynamics of septin ring and collar formation in Saccharomyces cerevisiae
    Hsin Chen
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Biol Chem 392:689-97. 2011
    ..Implications for the mechanism of septin ring assembly are discussed...
  48. ncbi request reprint Microtubule organization: cell shape is destiny
    Steven B Haase
    Biology Department, Duke University, Durham, NC 27710, USA
    Curr Biol 17:R249-51. 2007
    ..The cylindrical cell shape automatically corrects spindle orientation errors, rendering a checkpoint unnecessary...
  49. pmc Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae
    J J Moskow
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 20:7559-71. 2000
    ....
  50. pmc Isolation and characterization of effector-loop mutants of CDC42 in yeast
    A S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 12:1239-55. 2001
    ..The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors...
  51. ncbi request reprint How do cells know what shape they are?
    Hui Kang
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, 27710, USA
    Curr Genet . 2016
    ..Together, these findings suggest a model for how cells may monitor aspects of their own shape to influence cell behavior...
  52. ncbi request reprint Formin' actin filament bundles
    Daniel J Lew
    Nat Cell Biol 4:E29-30. 2002
  53. pmc Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition
    Zongtian Tong
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Cell Biol 179:1375-84. 2007
    ..Thus, Cdc42 activators and GAPs establish concentric zones of action such that polarization is directed to occur adjacent to but not within the previous cell division site...
  54. ncbi request reprint IP7 guards the CDK gate
    John D York
    Nat Chem Biol 4:16-7. 2008
  55. ncbi request reprint The immortal strand hypothesis: how could it work?
    Daniel J Lew
    Cell 133:21-3. 2008