Gary F Gerard
Affiliation: Invitrogen Corporation
- Mutation detection using Surveyor nucleasePeter Qiu
Transgenomic, Gaithersburg, MD, USA
Biotechniques 36:702-7. 2004..The entire protocol can be performed in less than a day and is suitable for automated and high-throughput procedures...
- The role of template-primer in protection of reverse transcriptase from thermal inactivationGary F Gerard
Invitrogen Corporation, 7335 Executive Way, Frederick, MD 21704, USA
Nucleic Acids Res 30:3118-29. 2002..AMV RT binds much tighter to template- primer and has a much greater tendency to remain bound during cDNA synthesis than M-MLV RT and therefore is better protected from heat inactivation...
- Excess dNTPs minimize RNA hydrolysis during reverse transcriptionGary F Gerard
Invitrogen, Frederick, MD, USA
Biotechniques 33:984, 986, 988 passim. 2002
- Thermophilic bacterial DNA polymerases with reverse-transcriptase activityHarini Shandilya
Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008, USA
Extremophiles 8:243-51. 2004..Several enzymes showed significant reverse-transcriptase activity in the presence of Mg2+, particularly the polymerases from Bacillus caldolyticus EA1, Caldibacillus cellovorans CompA.2, and Clostridium stercorarium...