PAUL ENGLUND

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint RNAi libraries and kinetoplast DNA
    P T Englund
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe St, Baltimore, MD 21205, USA
    Biochem Soc Trans 33:1409-12. 2005
  2. ncbi request reprint Overexpression of a cytochrome b5 reductase-like protein causes kinetoplast DNA loss in Trypanosoma brucei
    Shawn A Motyka
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 281:18499-506. 2006
  3. ncbi request reprint The rotational dynamics of kinetoplast DNA replication
    Yanan Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD, USA
    Mol Microbiol 64:676-90. 2007
  4. pmc Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes
    Neta Milman
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 104:19250-5. 2007
  5. pmc Identification of a bacterial-like HslVU protease in the mitochondria of Trypanosoma brucei and its role in mitochondrial DNA replication
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California, United States of America
    PLoS Pathog 4:e1000048. 2008
  6. doi request reprint A passion for parasites
    Paul T Englund
    From the Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 289:33712-29. 2014
  7. pmc The killing of African trypanosomes by ethidium bromide
    Arnab Roy Chowdhury
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland, United States of America
    PLoS Pathog 6:e1001226. 2010
  8. pmc TbPIF5 is a Trypanosoma brucei mitochondrial DNA helicase involved in processing of minicircle Okazaki fragments
    Beiyu Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland, United States of America
    PLoS Pathog 5:e1000589. 2009
  9. pmc Intramitochondrial location and dynamics of Crithidia fasciculata kinetoplast minicircle replication intermediates
    M E Drew
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Cell Biol 153:735-44. 2001
  10. pmc Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect
    April M Clayton
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Eukaryot Cell 10:286-92. 2011

Research Grants

  1. STRUCTURE AND SYNTHESIS OF DNA
    PAUL ENGLUND; Fiscal Year: 2003
  2. Trypansome Surface Glycoproteins
    PAUL ENGLUND; Fiscal Year: 2006

Collaborators

  • Joseph Shlomai
  • E E C Agbo
  • Y Liu
  • Frederic Bringaud
  • Scott Landfear
  • Robert E Jensen
  • Shawn A Motyka
  • Beiyu Liu
  • Arnab Roy Chowdhury
  • Mark E Drew
  • Zhixing Zhao
  • James C Morris
  • Zefeng Wang
  • Jennifer L Guler
  • Gokben Yildirir
  • Michele M Klingbeil
  • Jennifer L Stephens
  • Keith Gull
  • Soo Hee Lee
  • April M Clayton
  • Julius Lukes
  • Terry K Smith
  • Jianyang Wang
  • Jack D Griffith
  • Ziyin Li
  • Megan E Lindsay
  • Neta Milman
  • Kimberly S Paul
  • Najib M El-Sayed
  • Jayleen Grams
  • M E Drew
  • Megan L Povelones
  • Eva Gluenz
  • Theresa A Shapiro
  • Gökhan Tolun
  • Rahul Bakshi
  • Valeria Pappas-Brown
  • Ching C Wang
  • Eva Kriegova
  • Derrick R Robinson
  • Derrick Robinson
  • Akhilesh Pandey
  • Dario Kalume
  • Henrik Molina
  • Reuben Sharma
  • Kimberly Edwards
  • Elisabet Caler
  • Jose Franco da Silveira
  • Carlos Renato Machado
  • David Horn
  • Hernan Lorenzi
  • Ellen Kindlund
  • Bjorn Andersson
  • Mark Carrington
  • Martin Pentony
  • Ulf Pettersson
  • Elizabeth A Worthey
  • Pieter de Jong
  • Hamid Darban
  • Peter J Myler
  • Jonathan Crabtree
  • Bill Wickstead
  • Brian Haas
  • David A Campbell
  • Laura Robertson
  • Owen White
  • Tamara Feldblyum
  • Jyoti Shetty
  • Alberto Carlos Frasch
  • Siri Nelson
  • Amber Seyler
  • Martti T Tammi
  • Christy Vogt
  • Grace Pai
  • Lena Aslund
  • Gautam Aggarwal
  • Rick Tarleton
  • Jose Luis Ramirez
  • Mihai Pop
  • Stephen Ochaya
  • Gholam Fazelina
  • Marcela Ferella
  • Jennifer Wortman
  • Esteban Bontempi
  • Susan Van Aken
  • Alan McKenna
  • Mariano J Levin
  • Lihua Hou
  • Richard McCulloch
  • Gaelle Blandin

Detail Information

Publications28

  1. ncbi request reprint RNAi libraries and kinetoplast DNA
    P T Englund
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe St, Baltimore, MD 21205, USA
    Biochem Soc Trans 33:1409-12. 2005
    ..To identify genes for more kDNA replication proteins, we are using an RNA interference library, which is the first forward genetic approach used for these parasites...
  2. ncbi request reprint Overexpression of a cytochrome b5 reductase-like protein causes kinetoplast DNA loss in Trypanosoma brucei
    Shawn A Motyka
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 281:18499-506. 2006
    ..Furthermore, the growth defect caused by overexpression of cytochrome b(5) reductase-like protein could be partially rescued by simultaneously overexpressing tryparedoxin...
  3. ncbi request reprint The rotational dynamics of kinetoplast DNA replication
    Yanan Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD, USA
    Mol Microbiol 64:676-90. 2007
    ..Whereas the C. fasciculata kinetoplast rotates, that from T. brucei oscillates. Kinetoplast motion of either type must facilitate orderly replication of this incredibly complex structure...
  4. pmc Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes
    Neta Milman
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 104:19250-5. 2007
    ....
  5. pmc Identification of a bacterial-like HslVU protease in the mitochondria of Trypanosoma brucei and its role in mitochondrial DNA replication
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California, United States of America
    PLoS Pathog 4:e1000048. 2008
    ..TbHslVU is a eubacterial protease identified in the mitochondria of a eukaryote. It has a novel function in regulating mitochondrial DNA replication that has never been observed in other organisms...
  6. doi request reprint A passion for parasites
    Paul T Englund
    From the Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 289:33712-29. 2014
    ..I decided to take a chance on kDNA. Little did I know then that I would devote the next forty years of my life to studying kDNA replication. ..
  7. pmc The killing of African trypanosomes by ethidium bromide
    Arnab Roy Chowdhury
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland, United States of America
    PLoS Pathog 6:e1001226. 2010
    ....
  8. pmc TbPIF5 is a Trypanosoma brucei mitochondrial DNA helicase involved in processing of minicircle Okazaki fragments
    Beiyu Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland, United States of America
    PLoS Pathog 5:e1000589. 2009
    ..This replication defect is associated with kinetoplast shrinkage and eventual slowing of cell growth. We propose that TbPIF5 unwinds RNA primers after lagging strand synthesis, thus facilitating processing of Okazaki fragments...
  9. pmc Intramitochondrial location and dynamics of Crithidia fasciculata kinetoplast minicircle replication intermediates
    M E Drew
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Cell Biol 153:735-44. 2001
    ..The final replication events, including primer removal, repair of many of the gaps, and reattachment of the progeny minicircles to the network periphery, are thought to take place within the antipodal sites...
  10. pmc Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect
    April M Clayton
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Eukaryot Cell 10:286-92. 2011
    ..We further speculate that this compositional change affects TAC function, and thus kDNA segregation...
  11. pmc Role of p38 in replication of Trypanosoma brucei kinetoplast DNA
    Beiyu Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe St, Baltimore, MD 21205, USA
    Mol Cell Biol 26:5382-93. 2006
    ..p38 binds to minicircle sequences within the replication origin. We conclude that cells with RNAi-induced loss of p38 cannot initiate minicircle replication, although they can extensively unwind free minicircles...
  12. pmc p166, a link between the trypanosome mitochondrial DNA and flagellum, mediates genome segregation
    Zhixing Zhao
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    EMBO J 27:143-54. 2008
    ..Thus, p166 is the first reported molecular component of the TAC, and its discovery will facilitate study of kDNA segregation machinery at the molecular level...
  13. ncbi request reprint Fellowship of the rings: the replication of kinetoplast DNA
    Beiyu Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    Trends Parasitol 21:363-9. 2005
    ..The replication of the kDNA network is more complex than previously thought, and the discovery of new proteins involved in this process is currently the best approach for illuminating the replication mechanism...
  14. ncbi request reprint Integration of pZJM library plasmids into unexpected locations in the Trypanosoma brucei genome
    Shawn A Motyka
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Biochem Parasitol 134:163-7. 2004
  15. pmc Effect of hydroxyurea on procyclic Trypanosoma brucei: an unconventional mechanism for achieving synchronous growth
    Arnab Roy Chowdhury
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Eukaryot Cell 7:425-8. 2008
    ..The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited...
  16. ncbi request reprint Multiple mitochondrial DNA polymerases in Trypanosoma brucei
    Michele M Klingbeil
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland 21205, USA
    Mol Cell 10:175-86. 2002
    ..While typical mitochondria contain one DNA polymerase, pol gamma, trypanosome mitochondria contain five such enzymes, including the previously characterized pol beta...
  17. ncbi request reprint A trypanosome mitochondrial RNA polymerase is required for transcription and replication
    Jayleen Grams
    Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 277:16952-9. 2002
    ..Thus, based on sequence comparison and functional studies, we have cloned an mtRNAP from trypanosomes...
  18. pmc Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei
    Jennifer L Guler
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Mol Microbiol 67:1125-42. 2008
    ..Thus, we conclude that the mitochondrial synthase produces fatty acids needed for maintaining local phospholipid levels that are required for activity of respiratory complexes and preservation of mitochondrial morphology and function...
  19. ncbi request reprint Mitochondrial fatty acid synthesis in Trypanosoma brucei
    Jennifer L Stephens
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:4427-36. 2007
    ..Trypanosomes employ two FAS systems: the unconventional ELO pathway that synthesizes bulk fatty acids and a mitochondrial pathway that synthesizes specialized fatty acids that are likely utilized intramitochondrially...
  20. pmc Kinetoplast DNA network: evolution of an improbable structure
    Julius Lukes
    Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice
    Eukaryot Cell 1:495-502. 2002
  21. ncbi request reprint The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNA interference library
    Mark E Drew
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    J Biol Chem 278:46596-600. 2003
    ..brucei phosphoglycerate kinase activity in vitro with an IC50 of 7.5 microm. We conclude that 5 microm tubercidin kills trypanosomes by targeting glycolysis, especially by inhibition of phosphoglycerate kinase...
  22. pmc Closing the gaps in kinetoplast DNA network replication
    Michele M Klingbeil
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    Proc Natl Acad Sci U S A 101:4333-4. 2004
  23. ncbi request reprint RNA interference for analysis of gene function in trypanosomatids
    Shawn A Motyka
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N Wolfe St, Baltimore, Maryland 21205, USA
    Curr Opin Microbiol 7:362-8. 2004
    ..Finally, the creation of an RNA interference-based library has allowed, for the first time, an approach for conducting forward genetic experiments in this organism...
  24. ncbi request reprint The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  25. ncbi request reprint Effects of RNA interference of Trypanosoma brucei structure-specific endonuclease-I on kinetoplast DNA replication
    Yanan Liu
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland 21205, USA
    J Biol Chem 280:35513-20. 2005
    ..These effects ultimately led to shrinkage and loss of the kinetoplast DNA network and cessation of growth of the cell...
  26. pmc Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library
    James C Morris
    Department of Biological Chemistry, Johns Hopkins Medical School, 725 N Wolfe Street, Baltimore, MD 21205, USA
    EMBO J 21:4429-38. 2002
    ..These data suggest that T.brucei 'senses' changes in glucose level and modulates procyclin expression accordingly...
  27. ncbi request reprint Fatty acid synthesis by elongases in trypanosomes
    Soo Hee Lee
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    Cell 126:691-9. 2006
    ..In blood, ELO3 downregulation favors myristate synthesis, whereas low concentrations of exogenous fatty acids in cultured parasites cause upregulation of the entire pathway, allowing the parasite to adapt to different environments...
  28. pmc Asymmetrical division of the kinetoplast DNA network of the trypanosome
    Zefeng Wang
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    EMBO J 21:4998-5005. 2002
    ..Therefore, the average network size gradually increased. During the network shrinkage and early stages of recovery, there were changes in the minicircle repertoire...

Research Grants2

  1. STRUCTURE AND SYNTHESIS OF DNA
    PAUL ENGLUND; Fiscal Year: 2003
    ..The final aim will be to study DNA repair in the mitochondrion of Crithidia fasciculata. ..
  2. Trypansome Surface Glycoproteins
    PAUL ENGLUND; Fiscal Year: 2006
    ..The third aim involves the mechanism of GPI myristoylation, by remodeling reactions. These experiments involve attempts to purify and study a myristoyl transferase, using biochemical and genetic techniques. ..