Kenneth Witwer

Summary

Affiliation: Johns Hopkins University School of Medicine
Location: Baltimore, USA

Publications

  1. doi Circulating microRNA biomarker studies: pitfalls and potential solutions
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD
    Clin Chem 61:56-63. 2015
  2. pmc MicroRNA regulation of IFN-beta protein expression: rapid and sensitive modulation of the innate immune response
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA
    J Immunol 184:2369-76. 2010
  3. pmc A plasma microRNA signature of acute lentiviral infection: biomarkers of central nervous system disease
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    AIDS 25:2057-67. 2011
  4. pmc Induction of innate immune responses by SIV in vivo and in vitro: differential expression and function of RIG-I and MDA5
    Juliene G Co
    Johns Hopkins School of Medicine, Department of Molecular and Comparative Pathobiology, Baltimore, MD 21205, USA
    J Infect Dis 204:1104-14. 2011
  5. pmc Coordinated regulation of SIV replication and immune responses in the CNS
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 4:e8129. 2009
  6. pmc Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis
    Dillon C Muth
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    BMC Vet Res 11:187. 2015
  7. pmc A benchmark for microRNA quantification algorithms using the OpenArray platform
    Matthew N McCall
    Department of Biostatistics and Computational Biology, University of Rochester, 265 Crittenden Blvd, Rochester, 14642, NY, USA
    BMC Bioinformatics 17:138. 2016
  8. pmc SIV replication is directly downregulated by four antiviral miRNAs
    Jeanne M Sisk
    Department of Molecular and Comparative Pathobiology, Edward D, Miller Research Building, The Johns Hopkins University School of Medicine, 733 N Broadway, Baltimore, MD 21205, USA
    Retrovirology 10:95. 2013
  9. pmc Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: limited evidence for general uptake of dietary plant xenomiRs
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology The Johns Hopkins University School of Medicine Baltimore, ME USA
    RNA Biol 10:1080-6. 2013
  10. pmc Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, MD, USA
    J Extracell Vesicles 2:. 2013

Collaborators

  • Janice E Clements
  • Lucio Gama
  • Ming Li
  • Jeanne M Sisk
  • Melissa A McAlexander
  • Matthew N McCall
  • Dillon C Muth
  • Jonathan Liu
  • Juliene G Co
  • Alexander Crits-Christoph
  • Alexander S Baras
  • Marc K Halushka
  • Roxann Ingersoll
  • Kelly A Metcalf Pate
  • Jessica M Izzi
  • Robert J Adams
  • Sarah E Beck
  • Nathan M Pate
  • Lauren J Ostrenga
  • Baktiar O Karim
  • Maggie J Phillips
  • M Christine Zink

Detail Information

Publications20

  1. doi Circulating microRNA biomarker studies: pitfalls and potential solutions
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD
    Clin Chem 61:56-63. 2015
    ..Despite the promise of miRNA biomarkers, evaluation of the diagnostic specificity and reproducibility of reported markers suggests that realizing this promise remains a work in progress...
  2. pmc MicroRNA regulation of IFN-beta protein expression: rapid and sensitive modulation of the innate immune response
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA
    J Immunol 184:2369-76. 2010
    ..Thus, miRNAs may contribute significantly to the regulation of IFN-beta in innate immune responses...
  3. pmc A plasma microRNA signature of acute lentiviral infection: biomarkers of central nervous system disease
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    AIDS 25:2057-67. 2011
    ....
  4. pmc Induction of innate immune responses by SIV in vivo and in vitro: differential expression and function of RIG-I and MDA5
    Juliene G Co
    Johns Hopkins School of Medicine, Department of Molecular and Comparative Pathobiology, Baltimore, MD 21205, USA
    J Infect Dis 204:1104-14. 2011
    ..We have shown for the first time to our knowledge the functional role of MDA5 in the innate immune response to SIV infection...
  5. pmc Coordinated regulation of SIV replication and immune responses in the CNS
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 4:e8129. 2009
    ..These studies further suggest that interventions should target HIV-infected individuals with increased CCL2 levels or HIV RNA in the CNS...
  6. pmc Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis
    Dillon C Muth
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    BMC Vet Res 11:187. 2015
    ..Little is known about EVs in the cervicovaginal compartment, including the effects of reproductive tract disease on quantity and quality of EVs...
  7. pmc A benchmark for microRNA quantification algorithms using the OpenArray platform
    Matthew N McCall
    Department of Biostatistics and Computational Biology, University of Rochester, 265 Crittenden Blvd, Rochester, 14642, NY, USA
    BMC Bioinformatics 17:138. 2016
    ..While substantial research has been devoted to the development of methods to quantify mRNA expression, relatively little effort has been spent on microRNA expression...
  8. pmc SIV replication is directly downregulated by four antiviral miRNAs
    Jeanne M Sisk
    Department of Molecular and Comparative Pathobiology, Edward D, Miller Research Building, The Johns Hopkins University School of Medicine, 733 N Broadway, Baltimore, MD 21205, USA
    Retrovirology 10:95. 2013
    ..We examined whether specific host miRNAs directly target SIV RNA early in infection and might be induced via type I interferon pathways...
  9. pmc Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: limited evidence for general uptake of dietary plant xenomiRs
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology The Johns Hopkins University School of Medicine Baltimore, ME USA
    RNA Biol 10:1080-6. 2013
    ....
  10. pmc Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, MD, USA
    J Extracell Vesicles 2:. 2013
    ..On many points, consensus has not yet been achieved and must be built through the reporting of well-controlled experiments. ..
  11. doi Diet-responsive mammalian miRNAs are likely endogenous.
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology The Johns Hopkins University Baltimore, MD
    J Nutr 144:1880-1. 2014
  12. pmc miRNAs and SAMHD1 regulation in vitro and in a model of HIV CNS disease
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 North Broadway, BRB Suite 831, Baltimore, MD, 21025, USA
    J Neuroinflammation 12:159. 2015
    ..However, data from an animal model of HIV neurologic disorders may not be consistent with robust miRNA-mediated regulation of SAMHD1 in vivo. ..
  13. pmc Tristetraprolin expression and microRNA-mediated regulation during simian immunodeficiency virus infection of the central nervous system
    Jonathan Liu
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 N, Broadway, Miller Research Building Rm, 829, Baltimore, MD 21205, USA
    Mol Brain 6:40. 2013
    ..This control is lost during progression to disease. In this study, we assessed TTP regulation and association with cytokine regulation in the brain during SIV infection...
  14. pmc Comparison of Methods for miRNA Extraction from Plasma and Quantitative Recovery of RNA from Cerebrospinal Fluid
    Melissa A McAlexander
    Retrovirus Laboratory, Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine Baltimore, MD, USA
    Front Genet 4:83. 2013
    ....
  15. pmc Data submission and quality in microarray-based microRNA profiling
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Clin Chem 59:392-400. 2013
    ..Accordingly, database submission and provision of the Minimum Information About a Microarray Experiment (MIAME)(3) are required by most journals as a prerequisite for review or acceptance...
  16. pmc XenomiRs and miRNA homeostasis in health and disease: evidence that diet and dietary miRNAs directly and indirectly influence circulating miRNA profiles
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    RNA Biol 9:1147-54. 2012
    ..Further research into the dietary xenomiR hypothesis is needed to ensure rigor in the search for truly disease-specific miRNA biomarkers...
  17. pmc miRNA profiles of monocyte-lineage cells are consistent with complicated roles in HIV-1 restriction
    Jeanne M Sisk
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Viruses 4:1844-64. 2012
    ....
  18. pmc Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21025, USA
    Retrovirology 9:5. 2012
    ..Using samples from a well-characterized ES cohort, untreated viremic patients, and uninfected controls, we explored the PBMC miRNA profile and probed the relationships of miRNA expression, CD4+ T-cell counts, and viral load...