Brenda Oppert

Summary

Affiliation: Kansas State University
Country: USA

Publications

  1. ncbi request reprint Digestive proteinases of the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae)
    B Oppert
    USDA Agricultural Research Service, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    Bull Entomol Res 96:167-72. 2006
  2. doi request reprint Bacillus thuringiensis Cry3Aa protoxin intoxication of Tenebrio molitor induces widespread changes in the expression of serine peptidase transcripts
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, KS 66502, USA
    Comp Biochem Physiol Part D Genomics Proteomics 7:233-42. 2012
  3. pmc Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, Kansas, United States of America
    PLoS ONE 7:e34624. 2012
  4. doi request reprint Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors
    Brenda Oppert
    USDA ARS Center for Grain and Animal Health Research, 1515 College Ave, Manhattan, KS 66502, USA
    Comp Biochem Physiol Part D Genomics Proteomics 5:280-7. 2010
  5. doi request reprint Efficacy of Bacillus thuringiensis Cry3Aa protoxin and protease inhibitors against coleopteran storage pests
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, KS 66502, USA
    Pest Manag Sci 67:568-73. 2011
  6. ncbi request reprint Proteinase-mediated insect resistance to Bacillus thuringiensis toxins
    B Oppert
    United States Grain Marketing Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Manhattan, Kansas 66502 2736, USA
    J Biol Chem 272:23473-6. 1997
  7. ncbi request reprint Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Ave, Manhattan, KS 66502, USA
    Comp Biochem Physiol C Toxicol Pharmacol 134:481-90. 2003
  8. ncbi request reprint Two-dimensional analysis of proteinase activity
    Brenda Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    J Biochem Biophys Methods 67:173-9. 2006
  9. ncbi request reprint Digestive proteinases in Lasioderma serricorne (Coleoptera: Anobiidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    Bull Entomol Res 92:331-6. 2002
  10. ncbi request reprint Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, Manhattan, KS 66502, USA
    Comp Biochem Physiol C Toxicol Pharmacol 140:53-8. 2005

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Digestive proteinases of the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae)
    B Oppert
    USDA Agricultural Research Service, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    Bull Entomol Res 96:167-72. 2006
    ..angustus larvae. Results from these studies can be used to develop new control strategies to prevent damage to grains and stored products by C. angustus and similar coleopteran pests...
  2. doi request reprint Bacillus thuringiensis Cry3Aa protoxin intoxication of Tenebrio molitor induces widespread changes in the expression of serine peptidase transcripts
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, KS 66502, USA
    Comp Biochem Physiol Part D Genomics Proteomics 7:233-42. 2012
    ..Therefore, Bt intoxication significantly impacted the expression of serine peptidases, potentially important in protoxin processing, while the insect maintained the production of critical digestive cysteine peptidases...
  3. pmc Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, Kansas, United States of America
    PLoS ONE 7:e34624. 2012
    ..Data from this study represent the largest genetic sequence dataset for T. molitor to date. Furthermore, the methods in this study are useful for comparative analyses in organisms lacking a sequenced genome...
  4. doi request reprint Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors
    Brenda Oppert
    USDA ARS Center for Grain and Animal Health Research, 1515 College Ave, Manhattan, KS 66502, USA
    Comp Biochem Physiol Part D Genomics Proteomics 5:280-7. 2010
    ..These data suggest that T. castaneum larvae compensate for dietary cysteine protease inhibitors by altering large-scale gene expression patterns...
  5. doi request reprint Efficacy of Bacillus thuringiensis Cry3Aa protoxin and protease inhibitors against coleopteran storage pests
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, KS 66502, USA
    Pest Manag Sci 67:568-73. 2011
    ..Therefore, we evaluated the efficacy of a coleopteran-active toxin, Bacillus thuringiensis Cry3Aa, with or without protease inhibitors, in laboratory feeding assays against coleopteran storage pests...
  6. ncbi request reprint Proteinase-mediated insect resistance to Bacillus thuringiensis toxins
    B Oppert
    United States Grain Marketing Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Manhattan, Kansas 66502 2736, USA
    J Biol Chem 272:23473-6. 1997
    ....
  7. ncbi request reprint Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Ave, Manhattan, KS 66502, USA
    Comp Biochem Physiol C Toxicol Pharmacol 134:481-90. 2003
    ..These proteinaceous inhibitors are promising candidates for transgenic seed technology to reduce seed damage by T. castaneum...
  8. ncbi request reprint Two-dimensional analysis of proteinase activity
    Brenda Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    J Biochem Biophys Methods 67:173-9. 2006
    ..1 to 7.3. Using the 2-DE activity blot method, information was obtained on the relative number and physical properties of serine proteinases in a mixture of insect gut proteinases without prior fractionation...
  9. ncbi request reprint Digestive proteinases in Lasioderma serricorne (Coleoptera: Anobiidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    Bull Entomol Res 92:331-6. 2002
    ..The data suggest that protein digestion in L. serricorne is primarily dependent on trypsin- and chymotrypsin-like proteinases...
  10. ncbi request reprint Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
    B Oppert
    USDA ARS Grain Marketing and Production Research Center, Manhattan, KS 66502, USA
    Comp Biochem Physiol C Toxicol Pharmacol 140:53-8. 2005
    ..castaneum larvae on midgut proteolytic activity and beetle developmental time is achieved through the prevention of the adaptive proteolytic response to overcome the activity of either type of inhibitor...
  11. ncbi request reprint Characterization of cDNAs encoding three trypsin-like proteinases and mRNA quantitative analysis in Bt-resistant and -susceptible strains of Ostrinia nubilalis
    Huarong Li
    Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
    Insect Biochem Mol Biol 35:847-60. 2005
    ....
  12. ncbi request reprint Susceptibility of dipel-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae) to individual Bacillus thuringiensis protoxins
    Huarong Li
    Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
    J Econ Entomol 98:1333-40. 2005
    ..054 and 0.162 microg/ml. However, growth inhibition with Cry2Aa was not significant at either dose. These data provide information on the spectrum of resistance and cross-resistance to individual Cry protoxins in this strain...
  13. pmc Characterization of cDNAs encoding serine proteases and their transcriptional responses to Cry1Ab protoxin in the gut of Ostrinia nubilalis larvae
    Jianxiu Yao
    Department of Entomology, Kansas State University, Manhattan, Kansas, USA
    PLoS ONE 7:e44090. 2012
    ..nubilalis larvae and demonstrate differential expression of protease genes that may be related to Cry1Ab intoxication and/or resistance...
  14. ncbi request reprint DiPel-selected Ostrinia nubilalis larvae are not resistant to transgenic corn expressing Bacillus thuringiensis Cry1Ab
    Huarong Li
    Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA
    J Econ Entomol 100:1862-70. 2007
    ..Therefore, resistant insect populations with reduced proteinase activity do not seem to pose a threat to the efficacy of commercial MON810 and Bt11 corn hybrids...
  15. doi request reprint Tribolium castaneum larval gut transcriptome and proteome: A resource for the study of the coleopteran gut
    Kaley Morris
    Department of Biochemistry, Kansas State University, Manhattan, 66506, USA
    J Proteome Res 8:3889-98. 2009
    ..These data serve as a resource of T. castaneum transcripts and proteins in the larval gut and provide the basis for comparative transcriptomic and proteomic studies related to the gut of coleopteran insects...
  16. pmc Identification, RNAi knockdown, and functional analysis of an ejaculate protein that mediates a postmating, prezygotic phenotype in a cricket
    Jeremy L Marshall
    Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America
    PLoS ONE 4:e7537. 2009
    ..These findings represent one of the first studies to identify a protein that plays a role in mediating both a postmating, prezygotic isolation pathway and reproductive senescence...
  17. ncbi request reprint Binding analyses of Cry1Ab and Cry1Ac with membrane vesicles from Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis
    Huarong Li
    Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
    Biochem Biophys Res Commun 323:52-7. 2004
    ..nubilalis. Overall, the binding analyses indicate that resistance to Cry1Ab and Cry1Ac in this Bt-resistant strain of O. nubilalis is not associated with a loss of toxin binding...
  18. doi request reprint Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge
    Jeffrey C Lord
    USDA ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
    J Microbiol Methods 80:219-21. 2010
    ..Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems...
  19. doi request reprint Evaluation of reference genes for quantitative polymerase chain reaction across life cycle stages and tissue types of Tribolium castaneum
    Michelle J Toutges
    Center for Grain and Animal Health Research, Agricultural Research Service, U S Department of Agriculture, 1515 College Avenue, Manhattan, Kansas 66502, USA
    J Agric Food Chem 58:8948-51. 2010
    ....
  20. pmc Analysis of transcriptome data in the red flour beetle, Tribolium castaneum
    Yoonseong Park
    Department of Entomology, Kansas State University, Manhattan, KS 66506 4004, USA
    Insect Biochem Mol Biol 38:380-6. 2008
    ..We conservatively estimate that the current EST set represents more than 7,500 transcription units...
  21. pmc Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function
    Lindsey Perkin
    Center for Grain and Animal Health Research, USDA, Agricultural Research Service, Manhattan, KS, United States
    Peerj 4:e1581. 2016
    ..castaneum larvae and adults use for food digestion, and thus new potential targets for biologically-based control products. ..
  22. pmc Geographic distributions of Idh-1 alleles in a cricket are linked to differential enzyme kinetic performance across thermal environments
    Diana L Huestis
    Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
    BMC Evol Biol 9:113. 2009
    ..Here, we use enzyme kinetics to further explore GxE between Idh-1 genotype and temperature, and test the predictions of kinetic activity expected under drift or selection...
  23. pmc Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum
    Brenda Oppert
    USDA Agricultural Research Service, Center for Grain and Animal Health Research, Manhattan, KS, USA
    BMC Genomics 16:968. 2015
    ..Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide...
  24. ncbi request reprint Localization of post-proline cleaving peptidases in Tenebrio molitor larval midgut
    Irina A Goptar
    Chemical Faculty, Moscow State University, Moscow, Russia
    Biochimie 90:508-14. 2008
    ..6-8.6 and was maximal at pH 7.4. The sensitivity of PPCP2 to inhibitors and the effect of pH are similar to prolyl oligopeptidases with a cysteine residue near the substrate binding site...
  25. ncbi request reprint Insect resistance to Bacillus thuringiensis: alterations in the indianmeal moth larval gut proteome
    Mehmet Candas
    Biological Targets, Inc, Tioga, Texas 76271, USA
    Mol Cell Proteomics 2:19-28. 2003
    ....
  26. ncbi request reprint Molecular cloning of trypsin-like cDNAs and comparison of proteinase activities in the salivary glands and gut of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae)
    Yu Cheng Zhu
    USDA ARS JWDSRC, PO Box 346, Stoneville, MS 38776, USA
    Insect Biochem Mol Biol 33:889-99. 2003
    ..lineolaris...
  27. ncbi request reprint Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae)
    Huarong Li
    Department of Entomology, Kansas State University, Manhattan 66506, USA
    Insect Biochem Mol Biol 34:753-62. 2004
    ..Reduced protoxin processing due to decreased activities of Bt protoxin activation proteinases may be associated with resistance to Bt toxin in this resistant strain of O. nubilalis...
  28. ncbi request reprint An overlay technique for postelectrophoretic analysis of proteinase spectra in complex mixtures using p-nitroanilide substrates
    Konstantin S Vinokurov
    Department of Entomology, Biological Faculty, Moscow State University, Leninskie Gory, Moscow 119992, Russia
    Anal Biochem 337:164-6. 2005
  29. ncbi request reprint Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [Mayetiola destructor (Say)]
    Yu Cheng Zhu
    USDA ARS JWDSRC, PO Box 346 141 Exp Stn Rd, Stoneville, MS 38776, USA
    Insect Biochem Mol Biol 35:23-32. 2005
    ....
  30. ncbi request reprint Differences in susceptibility and physiological fitness of Mexican field Trichoplusia ni strains exposed to Bacillus thuringiensis
    Patricia Tamez-Guerra
    Departamento de Inmunología y Virología, FCB, UANL, A P 46 F San Nicolás de los Garza, NL Mexico
    J Econ Entomol 99:937-45. 2006
    ..The relationship of T. ni susceptibilities to Bt Cry toxins and protoxins after several generations of exposure to XenTari and its similarity to P. xylostella behavior...
  31. ncbi request reprint Analysis of midgut proteinases from Bacillus thuringiensis-susceptible and -resistant Heliothis virescens (Lepidoptera: Noctuidae)
    Lohitash Karumbaiah
    Department of Entomology, University of Georgia, Athens, GA 30602, USA
    Comp Biochem Physiol B Biochem Mol Biol 146:139-46. 2007
    ..Because serine proteinases are a critical component of Bt toxin mode of action, these differences may contribute to decreased toxicity in the Bt-resistant strains...
  32. doi request reprint Arcelins from an Indian wild pulse, Lablab purpureus, and insecticidal activity in storage pests
    Sundaram Janarthanan
    Thiagarajar Center for Entomological Science, Department of Zoology, Thiagarajar College Autonomous, Madurai 625009, India
    J Agric Food Chem 56:1676-82. 2008
    ..purpureus extract have the potential to control storage pests in cereals transformed with L. purpureus defense-related genes, but the need for more studies regarding efficacy and safety is discussed...
  33. doi request reprint The genome of the model beetle and pest Tribolium castaneum
    Stephen Richards
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 452:949-55. 2008
    ..Systemic RNA interference in T. castaneum functions differently from that in Caenorhabditis elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control...