Arshak Alexanian

Summary

Affiliation: Medical College of Wisconsin
Location: Milwaukee, USA
URL: http://www.mcw.edu/neurosurgery/faculty/Non-SurgFaculty/alexanian.htm
Summary:
The main focus of my current research is to understand the genetic and epigenetic mechanisms governing cell plasticity (proliferation, lineage commitment and differentiation) and application of these findings for regenerative medicine
Publications:
1.Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats.
Alexanian AR, Fehlings MG, Zhang Z, Maiman DJ.
Neurorehabil Neural Repair. 2011 Nov;25(9):873-80. Epub 2011 Aug 15.
PMID:[PubMed - in process]
Related citations

2.Feline bone marrow-derived mesenchymal stem cells express several pluripotent and neural markers and easily turn into neural-like cells by manipulation with chromatin modifying agents and neural inducing factors.
Zhang Z, Maiman DJ, Kurpad SN, Crowe MJ, Alexanian AR.
Cell Reprogram. 2011 Oct;13(5):385-90. Epub 2011 Jun 30.
PMID:[PubMed - in process]
Related citations

3.Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats.
Alexanian AR, Kwok WM, Pravdic D, Maiman DJ, Fehlings MG.
Restor Neurol Neurosci. 2010;28(6):761-7.
PMID:[PubMed - indexed for MEDLINE]
Related citations

4.An efficient method for generation of neural-like cells from adult human bone marrow-derived mesenchymal stem cells.
Alexanian AR.
Regen Med. 2010 Nov;5(6):891-900.
PMID:[PubMed - indexed for MEDLINE]
Related citations

5.Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia.
Alexanian AR, Svendsen CN, Crowe MJ, Kurpad SN.
Cytotherapy. 2011 Jan;13(1):61-8. Epub 2010 Aug 24.
PMID:[PubMed - indexed for MEDLINE]
Related citations

6.In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment.
Alexanian AR, Maiman DJ, Kurpad SN, Gennarelli TA.
Stem Cells Dev. 2008 Dec;17(6):1123-30.
PMID:[PubMed - indexed for MEDLINE]
Related citations

7.Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment.
Alexanian AR.
J Cell Biochem. 2007 Feb 1;100(2):362-71.
PMID:[PubMed - indexed for MEDLINE]
Related citations

8.Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury.
Macias MY, Syring MB, Pizzi MA, Crowe MJ, Alexanian AR, Kurpad SN.
Exp Neurol. 2006 Oct;201(2):335-48. Epub 2006 Jul 12.
PMID:[PubMed - indexed for MEDLINE]
Related citations

9.Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions.
Alexanian AR.
Exp Cell Res. 2005 Nov 1;310(2):383-91. Epub 2005 Oct 5.
PMID:[PubMed - indexed for MEDLINE]
Related citations

10.Efficient differentiation and integration of lineage-restricted neural precursors in the traumatically injured adult cat spinal cord.
Alexanian AR, Crowe MJ, Kurpad SN.
J Neurosci Methods. 2006 Jan 15;150(1):41-6. Epub 2005 Aug 8.
PMID:[PubMed - indexed for MEDLINE]
Related citations

11.Quiescent neural cells regain multipotent stem cell characteristics influenced by adult neural stem cells in co-culture.
Alexanian AR, Kurpad SN.
Exp Neurol. 2005 Jan;191(1):193-7.
PMID:[PubMed - indexed for MEDLINE]
Related citations

12.Differentiating adult hippocampal stem cells into neural crest derivatives.
Alexanian AR, Sieber-Blum M.
Neuroscience. 2003;118(1):1-5.
PMID:[PubMed - indexed for MEDLINE]
Related citations

13.Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons.
Alexanian AR, Bamburg JR, Hidaka H, Mornet D.
Cell Mol Neurobiol. 2001 Oct;21(5):437-51.
PMID:[PubMed - indexed for MEDLINE]
Related citations

14.Proliferation and regeneration of retrogradely labeled adult rat corticospinal neurons in culture.
Alexanian AR, Nornes HO.
Exp Neurol. 2001 Aug;170(2):277-82.
PMID:[PubMed - indexed for MEDLINE]
Related citations

15.Reversal of drug resistance in sarcoma-45 by the new calmodulin antagonist--trihydrochloride of [1,2,5-trimethyl-4-phenyl-4-beta-[N-(beta-ethylamino)-N-4-methoxybe nzy l]-ethylamino] piperidine (AR-2).
Alexanian AR, Arutyunian NS.
Invest New Drugs. 1999;17(2):105-10.
PMID:[PubMed - indexed for MEDLINE]
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16.Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB.
Alexanian AR, Bamburg JR.
FASEB J. 1999 Sep;13(12):1611-20.
PMID:[PubMed - indexed for MEDLINE] Free Article
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17.Antiarrhythmic action of the new calcium antagonist [1,2,5-trimethyl-4-phenyl-4-beta-(N-cyanoethyl-N-4-methoxybenzyl) -ethylamino]piperidine dihydrochloride.
Alexanian AR, Arutyunian NS, Galoyan AA.
Arzneimittelforschung. 1996 Dec;46(12):1110-3.
PMID:9006783[PubMed - indexed for MEDLINE]
Related citations

Publications

  1. ncbi request reprint Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury
    Melissa Y Macias
    Department of Neurosurgery, Neuroscience Research Laboratories, Medical College of Wisconsin and Clement J Zablocki VA Medical Center, Milwaukee, WI 53226, USA
    Exp Neurol 201:335-48. 2006
  2. ncbi request reprint Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB
    A R Alexanian
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523 1870, USA
    FASEB J 13:1611-20. 1999
  3. doi request reprint Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats
    Arshak R Alexanian
    Medical College of Wisconsin, Milwaukee, WI 53295, USA
    Neurorehabil Neural Repair 25:873-80. 2011
  4. doi request reprint Feline bone marrow-derived mesenchymal stem cells express several pluripotent and neural markers and easily turn into neural-like cells by manipulation with chromatin modifying agents and neural inducing factors
    Zhiying Zhang
    Department of Neurosurgery, Neuroscience Research Laboratory, VA Medical Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53295, USA
    Cell Reprogram 13:385-90. 2011
  5. doi request reprint Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats
    Arshak R Alexanian
    Neuroscience Research Labs, Deptpartment of Neurosurgery, Medical College of Wisconsin, VAMC, Milwaukee, WI 53295, USA
    Restor Neurol Neurosci 28:761-7. 2010
  6. doi request reprint An efficient method for generation of neural-like cells from adult human bone marrow-derived mesenchymal stem cells
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, VA Medical Center Research 151, 5000 West National Avenue, Milwaukee, Wisconsin, WI 53295, USA
    Regen Med 5:891-900. 2010
  7. doi request reprint Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, Medical College of Wisconsin VAMC, 5000 W National Ave 151, Milwaukee, WI 53295, USA
    Cytotherapy 13:61-8. 2011
  8. doi request reprint In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295, USA
    Stem Cells Dev 17:1123-30. 2008
  9. ncbi request reprint Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment
    Arshak R Alexanian
    Department of Neurosurgery, Neuroscience Research Labs, Medical College of Wisconsin, VAMC, 5000 W National Ave 151, Milwaukee, Wisconsin 53295, USA
    J Cell Biochem 100:362-71. 2007
  10. ncbi request reprint Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions
    Arshak R Alexanian
    Neuroscience Research Labs, Dept of Neurosurgery, Medical College of Wisconsin, VAMC, 5000 W National Ave 151, Milwaukee, WI 53295, USA
    Exp Cell Res 310:383-91. 2005

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury
    Melissa Y Macias
    Department of Neurosurgery, Neuroscience Research Laboratories, Medical College of Wisconsin and Clement J Zablocki VA Medical Center, Milwaukee, WI 53226, USA
    Exp Neurol 201:335-48. 2006
    ..Both allodynia- and CGRP/GAP43-positive afferent sprouting were less in the C17.2/GDNF group compared to the C17.2 group, suggesting a possible protective or analgesic effect of GDNF on post-injury neuropathic pain...
  2. ncbi request reprint Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB
    A R Alexanian
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523 1870, USA
    FASEB J 13:1611-20. 1999
    ..We conclude that S100betabeta enhances the survival of chick embryo forebrain neurons through the activation of NF-kappaB...
  3. doi request reprint Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats
    Arshak R Alexanian
    Medical College of Wisconsin, Milwaukee, WI 53295, USA
    Neurorehabil Neural Repair 25:873-80. 2011
    ..Bone marrow (BM)-derived mesenchymal stem cells (MSCs) can differentiate into neural phenotypes and be isolated and expanded for autotransplantation with no risk of rejection...
  4. doi request reprint Feline bone marrow-derived mesenchymal stem cells express several pluripotent and neural markers and easily turn into neural-like cells by manipulation with chromatin modifying agents and neural inducing factors
    Zhiying Zhang
    Department of Neurosurgery, Neuroscience Research Laboratory, VA Medical Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53295, USA
    Cell Reprogram 13:385-90. 2011
    ..We concluded that the expression of pluripotency- and neural-associated genes in unmodified fMSCs make them more pliable for reprogramming into a neural fate by manipulation with chromatin modifying agents and neural inducing factors...
  5. doi request reprint Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats
    Arshak R Alexanian
    Neuroscience Research Labs, Deptpartment of Neurosurgery, Medical College of Wisconsin, VAMC, Milwaukee, WI 53295, USA
    Restor Neurol Neurosci 28:761-7. 2010
    ..The aim of this study was to determine whether transplanted NI-MSCs survive, differentiate, and integrate in injured spinal cord (ISC) rats and promote functional recovery...
  6. doi request reprint An efficient method for generation of neural-like cells from adult human bone marrow-derived mesenchymal stem cells
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, VA Medical Center Research 151, 5000 West National Avenue, Milwaukee, Wisconsin, WI 53295, USA
    Regen Med 5:891-900. 2010
    ..The main idea of this approach was the reactivation of pluripotency-associated genes in MSCs before exposing them to neural-inducing factors...
  7. doi request reprint Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, Medical College of Wisconsin VAMC, 5000 W National Ave 151, Milwaukee, WI 53295, USA
    Cytotherapy 13:61-8. 2011
    ..In this study, we aimed to elucidate the therapeutic effect of different neuronal and glial progenitor populations of hNPC on locomotor and sensory functions of spinal cord-injured (SCI) rats...
  8. doi request reprint In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295, USA
    Stem Cells Dev 17:1123-30. 2008
    ....
  9. ncbi request reprint Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment
    Arshak R Alexanian
    Department of Neurosurgery, Neuroscience Research Labs, Medical College of Wisconsin, VAMC, 5000 W National Ave 151, Milwaukee, Wisconsin 53295, USA
    J Cell Biochem 100:362-71. 2007
    ..We concluded that the manipulation strategy used in this study can be a useful method for efficient production of NSC-like cells from MSCs...
  10. ncbi request reprint Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions
    Arshak R Alexanian
    Neuroscience Research Labs, Dept of Neurosurgery, Medical College of Wisconsin, VAMC, 5000 W National Ave 151, Milwaukee, WI 53295, USA
    Exp Cell Res 310:383-91. 2005
    ..We conclude that juxtacrine interaction between NSCs and MSCs combined with soluble factors released from NSCs are important for generation of neural-like cells from bone-marrow-derived adherent MSCs...
  11. ncbi request reprint Efficient differentiation and integration of lineage-restricted neural precursors in the traumatically injured adult cat spinal cord
    Arshak R Alexanian
    Neuroscience Research Labs, Department of Neurosurgery, Medical College of Wisconsin, VAMC, 5000 W National Avenue 151, Milwaukee, WI 53295, USA
    J Neurosci Methods 150:41-6. 2006
    ..We conclude that a mixture of more restricted neural precursors may be better suited than highly immature NSCs for neural replacement strategies after central nervous system (CNS) injuries...
  12. ncbi request reprint Quiescent neural cells regain multipotent stem cell characteristics influenced by adult neural stem cells in co-culture
    Arshak R Alexanian
    Neuroscience Research Laboratory, Deparment of Neurosurgery, Medical College of Wisconsin, VAMC, 5000 W National Avenue 151, Building 70 D, Milwaukee, WI 53295, USA
    Exp Neurol 191:193-7. 2005
    ..We conclude that reactivation of quiescent neural cells can be initiated by NSC-associated short-range cues but not by cell fusion...
  13. ncbi request reprint Differentiating adult hippocampal stem cells into neural crest derivatives
    A R Alexanian
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Neuroscience 118:1-5. 2003
    ..The data suggest that neural crest cell-derived short-range cues that are recognized across species can instruct adult mHNSC to differentiate into neural crest phenotypes...
  14. ncbi request reprint Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons
    A R Alexanian
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, USA
    Cell Mol Neurobiol 21:437-51. 2001
    ..4. These findings suggest a specific calcium-dependent regulatory pathway for modulating actomyosin during growth cone motility...
  15. ncbi request reprint Proliferation and regeneration of retrogradely labeled adult rat corticospinal neurons in culture
    A R Alexanian
    Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA
    Exp Neurol 170:277-82. 2001
    ..The result shows that adult corticospinal tract neurons are capable of DNA synthesis, and our total cell counts with labeled cells counts further suggest that these cells undergo cell division...
  16. ncbi request reprint Reversal of drug resistance in sarcoma-45 by the new calmodulin antagonist--trihydrochloride of [1,2,5-trimethyl-4-phenyl-4-beta-[N-(beta-ethylamino)-N-4'-methoxybe nzy l]-ethylamino] piperidine (AR-2)
    A R Alexanian
    Biochemical Pharmacology in the Institute of Biochemisty, Armenian Academy of Sciences, Yerevan
    Invest New Drugs 17:105-10. 1999
    ..It is concluded that the effect of AR-2 can be explained by both hydrophobic and electrostatic interaction with a protein target (170 kDa P-glycoprotein) in resistant sarcoma-45 tumor cell's membrane...
  17. ncbi request reprint Antiarrhythmic action of the new calcium antagonist [1,2,5-trimethyl-4-phenyl-4-beta-(N-cyanoethyl-N-4'-methoxybenzyl) -ethylamino]piperidine dihydrochloride
    A R Alexanian
    Department of Neurohormonal Biochemistry, Institute of Biochemistry, Yerevan Republic of Armenia
    Arzneimittelforschung 46:1110-3. 1996
    ....
  18. doi request reprint Enhancing the efficiency of direct reprogramming of human mesenchymal stem cells into mature neuronal-like cells with the combination of small molecule modulators of chromatin modifying enzymes, SMAD signaling and cyclic adenosine monophosphate levels
    Arshak R Alexanian
    Neuroscience Research Laboratories, Department of Neurosurgery, VA Medical Center Research 151, Milwaukee, WI 53295, United States
    Int J Biochem Cell Biol 45:1633-8. 2013
    ..We concluded that specific combinations of small molecules that target specific cell signaling pathways and chromatin modifying enzymes could be a promising approach for manipulation of adult stem cell plasticity. ..