Gibert H Smith

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Mammary cancer and epithelial stem cells: a problem or a solution?
    Gilbert H Smith
    Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Breast Cancer Res 4:47-50. 2002
  2. doi request reprint Binuclear Cells in the Lactating Mammary Gland: New Insights on an Old Concept?
    Gilbert H Smith
    Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 37, Rm 1122A, 37 Convent Drive, Bethesda, MD, 20892, USA
    J Mammary Gland Biol Neoplasia 21:21-3. 2016
  3. pmc Embryonic stem cells are redirected to non-tumorigenic epithelial cell fate by interaction with the mammary microenvironment
    Corinne A Boulanger
    Mammary Stem Cell Biology Section, Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland, United States of America
    PLoS ONE 8:e62019. 2013
  4. pmc Human breast cancer cells are redirected to mammary epithelial cells upon interaction with the regenerating mammary gland microenvironment in-vivo
    Karen M Bussard
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e49221. 2012
  5. pmc Immortalized, pre-malignant epithelial cell populations contain long-lived, label-retaining cells that asymmetrically divide and retain their template DNA
    Karen M Bussard
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Breast Cancer Res 12:R86. 2010
  6. pmc Selective segregation of DNA strands persists in long-label-retaining mammary cells during pregnancy
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA
    Breast Cancer Res 10:R90. 2008
  7. pmc Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    David L Mack
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Breast Cancer Res 9:R42. 2007
  8. pmc Estrogen receptor-alpha and progesterone receptor are expressed in label-retaining mammary epithelial cells that divide asymmetrically and retain their template DNA strands
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA
    Breast Cancer Res 8:R49. 2006
  9. pmc Re-evaluation of mammary stem cell biology based on in vivo transplantation
    Gilbert H Smith
    Mammary Stem Cell Biology Section, Mammary Biology and Tumorigenesis Laboratory, CCR, NCI, 37 Convent Drive, Bldg 37, Rm 1106, National Institutes of Health, Bethesda, MD 20892 USA
    Breast Cancer Res 10:203. 2008
  10. ncbi request reprint Stem cells and mammary cancer in mice
    Gibert H Smith
    Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, MD 10892, USA
    Stem Cell Rev 1:215-23. 2005

Collaborators

Detail Information

Publications39

  1. pmc Mammary cancer and epithelial stem cells: a problem or a solution?
    Gilbert H Smith
    Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Breast Cancer Res 4:47-50. 2002
    ..Do these stem cells represent primary targets for mammary neoplasia? If so, what are the implications for prevention/therapy?..
  2. doi request reprint Binuclear Cells in the Lactating Mammary Gland: New Insights on an Old Concept?
    Gilbert H Smith
    Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 37, Rm 1122A, 37 Convent Drive, Bethesda, MD, 20892, USA
    J Mammary Gland Biol Neoplasia 21:21-3. 2016
    ..From a historical perspective, it is more likely that binuclear secretory cells in the lactating mammary gland are a consequence of the DNA synthesis requirement for lactation, rather than an essential element. ..
  3. pmc Embryonic stem cells are redirected to non-tumorigenic epithelial cell fate by interaction with the mammary microenvironment
    Corinne A Boulanger
    Mammary Stem Cell Biology Section, Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland, United States of America
    PLoS ONE 8:e62019. 2013
    ..In addition, in secondary transplants, ES cell progeny proliferate, contribute apparently normal mammary progeny, maintain their multipotency and do not produce teratomas...
  4. pmc Human breast cancer cells are redirected to mammary epithelial cells upon interaction with the regenerating mammary gland microenvironment in-vivo
    Karen M Bussard
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e49221. 2012
    ..This work demonstrates that cultured human breast cancer cells (metastatic and non-metastatic) respond developmentally to signals generated by the mouse mammary gland microenvironment during gland regeneration in-vivo...
  5. pmc Immortalized, pre-malignant epithelial cell populations contain long-lived, label-retaining cells that asymmetrically divide and retain their template DNA
    Karen M Bussard
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Breast Cancer Res 12:R86. 2010
    ..We hypothesized that long-lived epithelial cells are present in immortal, premalignant cell populations, undergo asymmetric division, retain their template DNA strands, and cycle both during allometric growth and during pregnancy...
  6. pmc Selective segregation of DNA strands persists in long-label-retaining mammary cells during pregnancy
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA
    Breast Cancer Res 10:R90. 2008
    ..Mouse mammary epithelium in nonpregnant mice contains long label-retaining epithelial cells (LREC) that divide asymmetrically and retain their template DNA strands. The role of LREC during alveogenesis has not been determined...
  7. pmc Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    David L Mack
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Breast Cancer Res 9:R42. 2007
    ..In addition, decreased expression of Int6/eIF3e is observed in approximately one third of all human breast carcinomas...
  8. pmc Estrogen receptor-alpha and progesterone receptor are expressed in label-retaining mammary epithelial cells that divide asymmetrically and retain their template DNA strands
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA
    Breast Cancer Res 8:R49. 2006
    ..Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA...
  9. pmc Re-evaluation of mammary stem cell biology based on in vivo transplantation
    Gilbert H Smith
    Mammary Stem Cell Biology Section, Mammary Biology and Tumorigenesis Laboratory, CCR, NCI, 37 Convent Drive, Bldg 37, Rm 1106, National Institutes of Health, Bethesda, MD 20892 USA
    Breast Cancer Res 10:203. 2008
    ..In the present article, these issues are reviewed and discussed to provide a greater understanding of the complexity of these multiplex interactions...
  10. ncbi request reprint Stem cells and mammary cancer in mice
    Gibert H Smith
    Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, MD 10892, USA
    Stem Cell Rev 1:215-23. 2005
    ..Retroviral marking via MMTV proviral insertion demonstrates that this process progresses from a single mammary epithelial cell that possesses all the features ascribed to tissue-specific stem cells...
  11. ncbi request reprint Mammary epithelial stem cells
    G H Smith
    Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bldg 10, Room 8B07, 9000 Rockville Pike, Bethesda, MD 20892 1750, USA
    Microsc Res Tech 52:190-203. 2001
    ..Microsc. Res. Tech. 52:190-203, 2001. Published 2001 Wiley-Liss, Inc...
  12. ncbi request reprint Mammary stem cells come of age, prospectively
    Gilbert H Smith
    Laboratory of Mammary Biology and Tumorigenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Trends Mol Med 12:287-9. 2006
    ..This technical advance opens the possibility to use similar methodologies to isolate and characterize human breast epithelial stem cells, and elucidate their role in regeneration and neoplasia...
  13. ncbi request reprint Label-retaining epithelial cells in mouse mammary gland divide asymmetrically and retain their template DNA strands
    Gilbert H Smith
    Laboratory of Mammary Gland Biology and Tumorigenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Development 132:681-7. 2005
    ....
  14. ncbi request reprint Mammary stem cell repertoire: new insights in aging epithelial populations
    Gilbert H Smith
    Mammary Biology Group, Basic Research Laboratory, National Cancer Institute, Bethesda, MD 20892, USA
    Mech Ageing Dev 123:1505-19. 2002
    ..Our evidence indicates that a single mammary cell may have the capacity to self-renew through five transplant generations. Even some sixth generation implants show vigorous growth...
  15. ncbi request reprint Mammary epithelial stem cells: transplantation and self-renewal analysis
    Gilbert H Smith
    Section for Mammary Stem Cell Biology, Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cell Prolif 36:3-15. 2003
    ..Serial transplant studies indicate that these progenitors have a significant but limited capacity for self-renewal...
  16. ncbi request reprint Putative epithelial stem cell loss corresponds with mammary growth senescence
    Gilbert H Smith
    Section for Mammary Stem Cell Biology, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, NIH, Bldg 10, Room 5B56, 9000 Rockville Pike, Bethesda, MD 20892 1750, USA
    Cell Tissue Res 310:313-20. 2002
    ..This observation provides further support for the conclusion that these ultrastructurally distinct mammary cells represent the mammary stem/progenitor cell population...
  17. ncbi request reprint Alveolar progenitor cells develop in mouse mammary glands independent of pregnancy and lactation
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Physiol 212:729-36. 2007
    ....
  18. pmc Stem cells and the mammary microenvironment
    Brian W Booth
    Section for Mammary Stem Cell Biology, Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Breast Dis 29:57-67. 2008
    ..This suggests the ascendancy of the microenvironment over the intrinsic nature of somatic stem cells...
  19. pmc The mammary microenvironment alters the differentiation repertoire of neural stem cells
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:14891-6. 2008
    ..This result supports the conclusion that tissue-specific signals emanating from the stroma and from the differentiated somatic cells of the mouse mammary gland can redirect the NSCs to produce cellular progeny committed to MEC fates...
  20. pmc Common integration sites for MMTV in viral induced mouse mammary tumors
    Robert Callahan
    Mammary Gland Biology and Tumorigenesis Laboratory, National Cancer Institute, Building 37 Room 1118A, MSC4254, Bethesda, MD 20892, USA
    J Mammary Gland Biol Neoplasia 13:309-21. 2008
    ..Retroviral marking via MMTV proviral insertion demonstrates that this process progresses from a single mammary epithelial cell that possesses all of the features ascribed to tissue-specific stem cells...
  21. ncbi request reprint TGFbeta1 and TGFalpha contrarily affect alveolar survival and tumorigenesis in mouse mammary epithelium
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institute of Health, Bethesda, MD, USA
    Int J Cancer 120:493-9. 2007
    ....
  22. pmc Reprogramming human cancer cells in the mouse mammary gland
    Karen M Bussard
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 70:6336-43. 2010
    ..This is the first demonstration that human cells are capable of recognizing the signals generated by the mouse mammary gland microenvironment present during gland regeneration in vivo...
  23. pmc A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences
    William Lowther
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, MD 20892, USA
    J Virol 79:10093-6. 2005
    ..Reverse transcriptase PCR results demonstrated that each of the viral insertions led to elevated expression of the presumed target flanking genes...
  24. pmc Netrin-1 can affect morphogenesis and differentiation of the mouse mammary gland
    Luigi Strizzi
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Cell Physiol 216:824-34. 2008
    ..These results suggest that Netrin-1 may facilitate functional differentiation of mammary epithelial cells and possibly affect the expression of Nanog and/or Cripto-1 in multipotent cells that may reside in the mammary gland...
  25. pmc Interaction with the mammary microenvironment redirects spermatogenic cell fate in vivo
    Corinne A Boulanger
    Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:3871-6. 2007
    ..Our results provide evidence for the ascendancy of the tissue microenvironment over the intrinsic nature of cells from an alternative adult tissue...
  26. pmc Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Exp Cell Res 316:422-32. 2010
    ..AREG knockdown inhibits mammosphere formation by duct-limited mammary progenitor cells but not lobule-limited mammary progenitor cells. These data demonstrate AREG mediates the function of a subset of mammary progenitor cells in vitro...
  27. ncbi request reprint Parity-induced mouse mammary epithelial cells are pluripotent, self-renewing and sensitive to TGF-beta1 expression
    Corinne A Boulanger
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, MD 20892, USA
    Oncogene 24:552-60. 2005
    ....
  28. ncbi request reprint Mammary epithelial cells are not able to undergo pregnancy-dependent differentiation in the absence of the helix-loop-helix inhibitor Id2
    Keiko Miyoshi
    Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Building 8, Room 101, 8 Center Drive, Bethesda, MD 20892, USA
    Mol Endocrinol 16:2892-901. 2002
    ..From these results, we conclude that, in the absence of Id2, mammary epithelial development is arrested at an early stage of pregnancy...
  29. pmc Reprogramming cell fates in the mammary microenvironment
    Corinne A Boulanger
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, MD 20892, USA
    Cell Cycle 8:1127-32. 2009
    ....
  30. pmc Biogenesis and function of mouse mammary epithelium depends on the presence of functional alpha-catenin
    Rashmi V Nemade
    Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 101, 8 Center Drive, Bethesda, MD 20892, USA
    Mech Dev 121:91-9. 2004
    ..Lastly, no tumors were detected in mammary tissue lacking alpha-catenin...
  31. ncbi request reprint Roles of transforming growth factor-alpha in mammary development and disease
    Brian W Booth
    Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Growth Factors 25:227-35. 2007
    ..TGFalpha has also been implicated in numerous disease states including forms of breast cancer. This minireview summarizes the basic biology of TGFalpha and its actions during normal and pathogenic development of the mammary epithelium...
  32. ncbi request reprint Stem cells and tissue homeostasis in mammary glands
    Robert B Clarke
    Breast Biology Group, University of Manchester, Christie Hospital, Manchester, M20 4BX UK
    J Mammary Gland Biol Neoplasia 10:1-3. 2005
  33. ncbi request reprint Parity-induced mammary epithelial cells facilitate tumorigenesis in MMTV-neu transgenic mice
    MaLinda D Henry
    Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pathology and Microbiology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Oncogene 23:6980-5. 2004
    ....
  34. ncbi request reprint Pregnancy and stem cell behavior
    Kay Uwe Wagner
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Mammary Gland Biol Neoplasia 10:25-36. 2005
    ..Using appropriate animal models, we further demonstrate that PI-MECs are cellular targets for pregnancy-enhanced mammary tumorigenesis...
  35. ncbi request reprint An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal
    Kay Uwe Wagner
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Rm 8009, Omaha, NE 68198 6805, USA
    Development 129:1377-86. 2002
    ..e. ductal morphogenesis and lobulogenesis). We propose that this parity-induced population contributes importantly to the biological differences between the mammary glands of parous and nulliparous females...
  36. ncbi request reprint Contributions of mouse biology to breast cancer research
    Robert D Cardiff
    University of California, Davis, USA
    Comp Med 52:12-31. 2002
  37. pmc Stem cells, hormones, and mammary cancer
    Gilbert H Smith
    Adv Exp Med Biol 617:69-78. 2008
  38. ncbi request reprint Expression of the whey acidic protein (Wap) is necessary for adequate nourishment of the offspring but not functional differentiation of mammary epithelial cells
    Aleata A Triplett
    Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Genesis 43:1-11. 2005
    ..Important implications of these findings for the use of Wap as a marker for advanced differentiation of mammary epithelial cells and the biology of pluripotent progenitors are discussed in the final section...
  39. ncbi request reprint Re: Immunocytochemical demonstration of caseins in mouse mammary glands
    Gilbert H Smith
    J Histochem Cytochem 50:997-8. 2002