Andrew L Harris

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. ncbi request reprint Emerging issues of connexin channels: biophysics fills the gap
    A L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, University Heights, Newark, NJ 07103, USA
    Q Rev Biophys 34:325-472. 2001
  2. pmc Connexin specificity of second messenger permeation: real numbers at last
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    J Gen Physiol 131:287-92. 2008
  3. pmc Connexin channel permeability to cytoplasmic molecules
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, Newark, NJ 07103, USA
    Prog Biophys Mol Biol 94:120-43. 2007
  4. ncbi request reprint Nature of Cx30-containing channels in the adult mouse mammary gland
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, UMDNJ, Newark, NJ 07103, USA
    Cell Tissue Res 328:97-107. 2007
  5. ncbi request reprint Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates
    Wafaa A Ayad
    Department of Pharmacology and Physiology, New Jersey Medical School, Newark, NJ 07103, USA
    J Biol Chem 281:16727-39. 2006
  6. ncbi request reprint Altered permeability and modulatory character of connexin channels during mammary gland development
    Darren Locke
    Department of Pharmacology and Physiology, UMDNJ, Newark, NJ 07101, USA
    Exp Cell Res 298:643-60. 2004
  7. pmc Mechanism for modulation of gating of connexin26-containing channels by taurine
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, USA
    J Gen Physiol 138:321-39. 2011
  8. pmc Connexin channels and phospholipids: association and modulation
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    BMC Biol 7:52. 2009
  9. ncbi request reprint Neutral, acidic, and basic derivatives of anthranilamide that confer different formal charge to reducing oligosaccharides
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, UMDNJ, 185 South Orange Avenue, Newark, NJ 07103, USA
    Carbohydr Res 339:221-31. 2004
  10. pmc Post-translational modifications of connexin26 revealed by mass spectrometry
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Biochem J 424:385-98. 2009

Research Grants

  1. MODULATION OF CONNEXIN CHANNEL ACTIVITY
    Andrew Harris; Fiscal Year: 2002
  2. PROPERTIES OF CONNEXIN CHANNELS THAT CAUSE DEAFNESS
    Andrew Harris; Fiscal Year: 2006
  3. PERMEABILITY MEDIATED BY CONNEXIN CHANNELS
    Andrew Harris; Fiscal Year: 2007
  4. STRUCTURE-FUNCTION OF CONNEXIN PORES
    Andrew Harris; Fiscal Year: 2009

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Emerging issues of connexin channels: biophysics fills the gap
    A L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, University Heights, Newark, NJ 07103, USA
    Q Rev Biophys 34:325-472. 2001
    ..However, the relation between these two mechanisms are unexplored, as are specific mechanisms of direct endogenous regulation of connexin channel activity. (ABSTRACT TRUNCATED)..
  2. pmc Connexin specificity of second messenger permeation: real numbers at last
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    J Gen Physiol 131:287-92. 2008
  3. pmc Connexin channel permeability to cytoplasmic molecules
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, Newark, NJ 07103, USA
    Prog Biophys Mol Biol 94:120-43. 2007
    ..At this time, the nature of those interactions is unclear. One hopes that with more detailed permeability and structural information, the specific molecular mechanisms of the selectivity can be elucidated...
  4. ncbi request reprint Nature of Cx30-containing channels in the adult mouse mammary gland
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, UMDNJ, Newark, NJ 07103, USA
    Cell Tissue Res 328:97-107. 2007
    ..Such changes in connexin expression and channel composition and their chemical modulation are discussed in relation to the various stages of mammary gland development in the adult mouse...
  5. ncbi request reprint Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates
    Wafaa A Ayad
    Department of Pharmacology and Physiology, New Jersey Medical School, Newark, NJ 07103, USA
    J Biol Chem 281:16727-39. 2006
    ..Dynamic regulation of channel composition in vivo may effectively and efficiently modulate intercellular signaling by inositol phosphates...
  6. ncbi request reprint Altered permeability and modulatory character of connexin channels during mammary gland development
    Darren Locke
    Department of Pharmacology and Physiology, UMDNJ, Newark, NJ 07101, USA
    Exp Cell Res 298:643-60. 2004
    ..Connexin channel stoichiometry, permeability, and chemical gating character change in precisely the desired fashion after parturition to maximize molecular and electrical coupling to support coordinated milk secretion...
  7. pmc Mechanism for modulation of gating of connexin26-containing channels by taurine
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, USA
    J Gen Physiol 138:321-39. 2011
    ..These studies reveal that AS disrupt a pH-driven cytoplasmic interdomain interaction in Cx26-containing channels, causing closure, and that the Cx26CT has a modulatory role in Cx26 function...
  8. pmc Connexin channels and phospholipids: association and modulation
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    BMC Biol 7:52. 2009
    ..The importance of interactions between connexin channels and phospholipids is poorly understood...
  9. ncbi request reprint Neutral, acidic, and basic derivatives of anthranilamide that confer different formal charge to reducing oligosaccharides
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, UMDNJ, 185 South Orange Avenue, Newark, NJ 07103, USA
    Carbohydr Res 339:221-31. 2004
    ..Furthermore, because glucosaccharides have rigid solution structure, these small fluorescent adducts with different formal charge are ideal tools for molecular sizing studies of membrane pores...
  10. pmc Post-translational modifications of connexin26 revealed by mass spectrometry
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Biochem J 424:385-98. 2009
    ..Knowledge of the PTMs of Cx26 will be instrumental in understanding how alterations in the cellular mechanisms of Cx26 channel biogenesis and function lead to losses in auditory function and disfiguring skin disorders...
  11. pmc Ca2+-dependent inactivation of CaV1.2 channels prevents Gd3+ block: does Ca2+ block the pore of inactivated channels?
    Olga Babich
    Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    J Gen Physiol 129:477-83. 2007
    ..A minimal model, in which the inactivation "gate" is an increase in affinity of the selectivity filter for permeating ions, successfully simulates the characteristic U-shaped voltage dependence of inactivation in Ca(2+)...
  12. pmc Tetracycline-regulated expression enables purification and functional analysis of recombinant connexin channels from mammalian cells
    Irina V Koreen
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, Newark, NJ 07103, USA
    Biochem J 383:111-9. 2004
    ..Furthermore, the generic cloning site of the new pBI-GT vector and the commercial availability of anti-haemagglutinin (clone HA-7)-agarose make this affinity tagging and purification procedure easily applicable to other proteins...
  13. ncbi request reprint Biochemical requirements for inhibition of Connexin26-containing channels by natural and synthetic taurine analogs
    Liang Tao
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Biol Chem 279:38544-54. 2004
    ..The finding that cytoplasmic aminosulfonates inhibit connexin channels whereas other cytoplasmic compounds antagonize the inhibition suggests that gap junction channels are regulated by a complex interplay of cytoplasmic ligands...
  14. ncbi request reprint Reversible pore block of connexin channels by cyclodextrins
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Biol Chem 279:22883-92. 2004
    ..CDs may be useful as molecular tools with which to explore the structure of the connexin pore and to alter molecular movement through connexin channels...
  15. pmc Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules
    Xuhui Tong
    Department of Pharmacology, Physiology and Neuroscience, New Jersey Medical School, Rutgers University, Newark, NJ 07103 Department of Pharmacology, Bengbu Medical College, Bengbu, Anhui Province 233000, China
    J Gen Physiol 146:245-54. 2015
    ..This potential for reversibility of thiol modification applies to on-cell accessibility studies of connexin channels and other channels that are permeable to large molecules, such as pannexin, CALHM, and VRAC. ..
  16. pmc Insights on the mechanisms of Ca(2+) regulation of connexin26 hemichannels revealed by human pathogenic mutations (D50N/Y)
    William Lopez
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    J Gen Physiol 142:23-35. 2013
    ..Our data provide a mechanistic understanding of how mutations at position 50 that cause human diseases are linked to dysfunction of hemichannel gating by external Ca(2+). ..
  17. ncbi request reprint Isoelectric points and post-translational modifications of connexin26 and connexin32
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, 185 South Orange Ave, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    FASEB J 20:1221-3. 2006
    ..Most of these modifications have not previously been identified for connexins and may be instrumental in guiding and understanding novel aspects of channel trafficking and molecular mechanisms of channel regulation...
  18. ncbi request reprint Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts
    Darren Locke
    Department of Pharmacology and Physiology, MSB I 645, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Avenue, Newark, New Jersey 07103, USA
    Biochemistry 44:13027-42. 2005
    ..Rafts may be involved in trafficking of plasma membrane connexin channels to gap junctions...
  19. pmc Mechanism of gating by calcium in connexin hemichannels
    William Lopez
    Department of Pharmacology, Physiology and Neuroscience, New Jersey Medical School, Rutgers University, Newark, NJ 07103
    Proc Natl Acad Sci U S A 113:E7986-E7995. 2016
    ....
  20. pmc The role of gap junction communication and oxidative stress in the propagation of toxic effects among high-dose α-particle-irradiated human cells
    Narongchai Autsavapromporn
    Department of Radiology, UMDNJ New Jersey Medical School Cancer Center, Newark, New Jersey 07103, USA
    Radiat Res 175:347-57. 2011
    ....
  21. ncbi request reprint Open pore block of connexin26 and connexin32 hemichannels by neutral, acidic and basic glycoconjugates
    Darren Locke
    Department of Pharmacology and Physiology, New Jersey Medical School, Newark, NJ 07103, USA
    Cell Commun Adhes 10:239-44. 2003
    ....
  22. pmc Voltage-sensing and substate rectification: moving parts of connexin channels
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, Newark, NJ 07103, USA
    J Gen Physiol 119:165-9. 2002
  23. pmc Gap junction channel structure in the early 21st century: facts and fantasies
    Mark Yeager
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Cell Biol 19:521-8. 2007
    ..An experimentally determined structure at atomic resolution will be essential to confirm and resolve these concepts...
  24. ncbi request reprint 2-aminoethoxydiphenyl borate directly inhibits channels composed of connexin26 and/or connexin32
    Liang Tao
    Department of Pharmacology, Zhongsan College of Medicine, Sun Yet san University, Guangzhou China 510080
    Mol Pharmacol 71:570-9. 2007
    ..These results show that protonated 2-APB directly and reversibly inhibits connexin channels composed of Cx26 and/or Cx32 and suggest involvement of the carboxyl-terminal domain...

Research Grants4

  1. MODULATION OF CONNEXIN CHANNEL ACTIVITY
    Andrew Harris; Fiscal Year: 2002
    ..Such defects will result in abnormal (i.e., greater or lesser) intercellular signaling molecules. The proposed studies address how this may occur. ..
  2. PROPERTIES OF CONNEXIN CHANNELS THAT CAUSE DEAFNESS
    Andrew Harris; Fiscal Year: 2006
    ..The information obtained will be informative regarding connexin channel function in other contexts, and will lead to insights about mechanisms of selectivity among cytoplasmic permeants. ..
  3. PERMEABILITY MEDIATED BY CONNEXIN CHANNELS
    Andrew Harris; Fiscal Year: 2007
    ..The information obtained will inform cellular and physiological studies of intercellular communication in disease and development. ..
  4. STRUCTURE-FUNCTION OF CONNEXIN PORES
    Andrew Harris; Fiscal Year: 2009
    ..In the present proposal, this analysis will be applied to Cx32 and Cx26, defects in which cause X-linked Charcot-Marie-Tooth disease neuropathy and sensorineural deafness, respectively. ..