J A Ramos-Vara

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. ncbi Glomerulocystic kidney disease in a Belgian Malinois dog: an ultrastructural, immunohistochemical, and lectin-binding study
    Jose A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, Indiana, USA
    Ultrastruct Pathol 28:33-42. 2004
  2. doi Immunohistochemical Detection of Pax8 and Napsin A in Canine Thyroid Tumours: Comparison with Thyroglobulin, Calcitonin and Thyroid Transcription Factor 1
    J A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, 406 South University St, West Lafayette, IN, USA Electronic address
    J Comp Pathol 155:286-298. 2016
  3. doi Immunohistochemical evaluation of the effects of paraffin section storage on biomarker stability
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:102-9. 2014
  4. doi Immunohistochemical expression of melanocytic antigen PNL2, Melan A, S100, and PGP 9.5 in equine melanocytic neoplasms
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathbiology, Purdue University, 406 South University St, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:161-6. 2014
  5. doi When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:42-87. 2014
  6. doi Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors
    A Durkes
    Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 49:834-8. 2012
  7. ncbi Pulmonary neoplasia in two llamas (Lama glama)
    J A Ramos-Vara
    Vet Pathol 41:520-3. 2004
  8. doi Immunohistochemical expression of E-cadherin does not distinguish canine cutaneous histiocytoma from other canine round cell tumors
    J A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 48:758-63. 2011
  9. doi Principles and methods of immunohistochemistry
    Jose A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 691:83-96. 2011
  10. doi Immunohistochemical identification of canine melanocytic neoplasms with antibodies to melanocytic antigen PNL2 and tyrosinase: comparison with Melan A
    J A Ramos-Vara
    Department of Comparative Pathbiology, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 48:443-50. 2011

Detail Information

Publications29

  1. ncbi Glomerulocystic kidney disease in a Belgian Malinois dog: an ultrastructural, immunohistochemical, and lectin-binding study
    Jose A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, Indiana, USA
    Ultrastruct Pathol 28:33-42. 2004
    ..The presence of parietal podocytes in all cysts suggests that abnormal differentiation may play an important role in the pathogenesis of this type of polycystic kidney disease...
  2. doi Immunohistochemical Detection of Pax8 and Napsin A in Canine Thyroid Tumours: Comparison with Thyroglobulin, Calcitonin and Thyroid Transcription Factor 1
    J A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, 406 South University St, West Lafayette, IN, USA Electronic address
    J Comp Pathol 155:286-298. 2016
    ..Thus, these markers are sensitive and, except for renal cell carcinoma (for Pax8, napsin A) and pulmonary adenocarcinoma (for napsin A), are specific thyroid tumour markers...
  3. doi Immunohistochemical evaluation of the effects of paraffin section storage on biomarker stability
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:102-9. 2014
    ..In conclusion, light-induced antigen decay (tissue section aging) is antigen dependent and could explain unexpectedly weak or negative immunohistochemical reactions in stored paraffin sections...
  4. doi Immunohistochemical expression of melanocytic antigen PNL2, Melan A, S100, and PGP 9.5 in equine melanocytic neoplasms
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathbiology, Purdue University, 406 South University St, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:161-6. 2014
    ..In summary, antibody PNL2 is a sensitive marker of equine melanocytic neoplasms and is more specific than S100 protein or PGP 9.5. In contrast, the monoclonal antibody to Melan A did not react with any of the equine melanomas. ..
  5. doi When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA Email
    Vet Pathol 51:42-87. 2014
    ....
  6. doi Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors
    A Durkes
    Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 49:834-8. 2012
    ....
  7. ncbi Pulmonary neoplasia in two llamas (Lama glama)
    J A Ramos-Vara
    Vet Pathol 41:520-3. 2004
    ..The diagnosis was adenosquamous carcinoma. Pulmonary tumors account for 23% of neoplasms in South American camelids in our laboratory, making this the second most common type of neoplasm after lymphosarcoma...
  8. doi Immunohistochemical expression of E-cadherin does not distinguish canine cutaneous histiocytoma from other canine round cell tumors
    J A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 48:758-63. 2011
    ....
  9. doi Principles and methods of immunohistochemistry
    Jose A Ramos-Vara
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 691:83-96. 2011
    ..Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized...
  10. doi Immunohistochemical identification of canine melanocytic neoplasms with antibodies to melanocytic antigen PNL2 and tyrosinase: comparison with Melan A
    J A Ramos-Vara
    Department of Comparative Pathbiology, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 48:443-50. 2011
    ..Results indicate that PNL2 is an excellent marker in the identification of canine melanomas and that the sensitivity is close to 100% when used in conjunction with Melan A and tyrosinase...
  11. doi Immunohistochemical detection of CD34, E-cadherin, claudin-1, glucose transporter 1, laminin, and protein gene product 9.5 in 28 canine and 8 feline meningiomas
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 47:725-37. 2010
    ..Further studies with CD34, E-cadherin, and claudin-1 in canine and feline tumors that may mimic meningiomas are needed to determine the adequacy of this approach...
  12. doi Immunohistochemical evaluation of GATA-4 in canine testicular tumors
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 46:893-6. 2009
    ..GCTs were negative for this marker. Sex cord-stromal cells of MGSCT were also positive. These results indicate that GATA-4 is mainly expressed in sex cord-stromal tumors and not in germ cell tumors of the canine testis...
  13. ncbi Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories
    Jose A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907, USA
    J Vet Diagn Invest 20:393-413. 2008
    ..Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories...
  14. doi Metritis, valvular endocarditis, and septicemia by Actinobacillus equuli in a gilt in the United States
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 45:495-9. 2008
    ..A. equuli in pigs in the United States has been reported only twice and not, to our knowledge, in the last 30 years. This is the first time that molecular techniques have been used to characterize this organism in FFPE porcine tissues...
  15. ncbi Immunohistochemical detection of multiple myeloma 1/interferon regulatory factor 4 (MUM1/IRF-4) in canine plasmacytoma: comparison with CD79a and CD20
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 44:875-84. 2007
    ....
  16. ncbi Usefulness of thyroid transcription factor-1 immunohistochemical staining in the differential diagnosis of primary pulmonary tumors of dogs
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 42:315-20. 2005
    ..On the basis of our limited studies and the human literature, it appears that MoAb 8G7G3/1 to TTF-1 is a highly specific (with the exception of thyroid tumors) and moderately sensitive marker for canine pulmonary epithelial tumors...
  17. ncbi Technical aspects of immunohistochemistry
    J A Ramos-Vara
    Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 42:405-26. 2005
    ..Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed...
  18. ncbi Nocardia tenerifensis genome identification in a cutaneous granuloma of a cat
    Jose A Ramos-Vara
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    J Vet Diagn Invest 19:577-80. 2007
    ..and had the highest sequence homology of 98.2% with Nocardia tenerifensis among Nocardia spp. To the authors' knowledge, this is the first report of detection of N. tenerifensis genome associated with cutaneous nocardiosis in an animal...
  19. ncbi Immunohistochemical detection of protein gene product 9.5 (PGP 9.5) in canine epitheliotropic T-cell lymphoma (mycosis fungoides)
    J A Ramos-Vara
    Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 44:74-9. 2007
    ..This unexpected staining of lymphocytes in mycosis fungoides with an antibody to PGP 9.5 demonstrates its presence in nonneuroendocrine tumors and precludes its use as the sole diagnostic marker in discrete cell tumors in the skin...
  20. ncbi Comparison of two polymer-based immunohistochemical detection systems: ENVISION+ and ImmPRESS
    Jose A Ramos-Vara
    Purdue University, Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 406 South University, West Lafayette, IN 47905, USA
    J Microsc 224:135-9. 2006
    ..The cost of ImmPRESS was 25% lower than for ENVISION+trade mark. Based on these results, ImmPRESS is a good polymer-based detection system for routine immunohistochemistry...
  21. ncbi Histologic and immunohistochemical characterization of a testicular mixed germ cell sex cord-stromal tumor and a leydig cell tumor in a dog
    M A Owston
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, 406 South University, West Lafayette, IN 47907 2065, USA
    Vet Pathol 44:936-43. 2007
    ..5, and melan A will help distinguish the three main cell types in canine testicular germ cell and sex cord-stromal tumors...
  22. ncbi Interstitial cell tumor and Sertoli cell tumor in the testis of a cat
    M A Miller
    Department of Veterinary Pathology, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 44:394-7. 2007
    ..Neither tumor had metastasized. The right testis was never found and was presumed to have been removed during the reported castration procedure...
  23. ncbi Testicular interstitial cell tumor and gynecomastia in a rabbit
    K A Maratea
    Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
    Vet Pathol 44:513-7. 2007
    ..This is the first report of concurrent interstitial cell tumor and gynecomastia in a rabbit and also the first description of gynecomastia in this species...
  24. doi Systemic sarcocystosis in a striped skunk (Mephitis mephitis)
    G N Burcham
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University Street, West Lafayette, IN 47907, USA
    Vet Pathol 47:560-4. 2010
    ..Additionally, canine distemper virus infection was confirmed with histopathology and immunohistochemistry. Concurrent canine distemper suggests that immunosuppression may have played a role in S neurona infection in this skunk...
  25. doi Comparison of CD34, CD31, and factor VIII-related antigen immunohistochemical expression in feline vascular neoplasms and CD34 expression in feline nonvascular neoplasms
    R N Jennings
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University, West Lafayette, IN 47907, USA
    Vet Pathol 49:532-7. 2012
    ..Given the broad expression of CD34 in mesenchymal neoplasms, this marker has limited diagnostic relevance for vascular neoplasms of cats...
  26. doi Randomized trial of cisplatin versus firocoxib versus cisplatin/firocoxib in dogs with transitional cell carcinoma of the urinary bladder
    D W Knapp
    Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907 2026, USA
    J Vet Intern Med 27:126-33. 2013
    ..Cox-2 is expressed in TCC, but only in limited sites within the kidney. A cox-2 inhibitor could enhance the antitumor activity of cisplatin with potentially fewer adverse effects on the kidney...
  27. ncbi Listeric meningoencephalomyelitis in a cougar (Felis concolor): characterization by histopathologic, immunohistochemical, and molecular methods
    I M Langohr
    Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907 2065, USA
    Vet Pathol 43:381-3. 2006
    ..On the basis of the nucleotide sequence of the 16S rRNA gene, the isolated strain was determined to be serotype 1/2a. Food-borne transmission of the bacterium was suspected, but food was not available for testing...
  28. doi Sequencing of the von Hippel-Lindau gene in canine renal carcinoma
    B M Pressler
    Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
    J Vet Intern Med 23:592-7. 2009
    ..The canine VHL coding deoxyribonucleic acid (DNA) shares 90% identity with the human VHL gene...
  29. doi Cerebellar abiotrophy in an alpaca (Lama pacos)
    P Mouser
    Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Vet Pathol 46:1133-7. 2009
    ..To the investigators' knowledge, this is the first report of cerebellar abiotrophy in a camelid and the first documentation of IHC findings associated with this condition...