I Dobrinski

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi Transplantation of germ cells and testis tissue for the study and preservation of fertility
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, 145 Myrin Bldg, New Bolton Center, School of Veterinary Medicine University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Soc Reprod Fertil Suppl 65:447-58. 2007
  2. ncbi Germ cell transplantation
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Semin Reprod Med 23:257-65. 2005
  3. ncbi Advances and applications of germ cell transplantation
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Hum Fertil (Camb) 9:9-14. 2006
  4. ncbi Male germ cell transplantation
    I Dobrinski
    School of Veterinary Medicine, Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, Kennett Square, PA 19348, USA
    Reprod Domest Anim 43:288-94. 2008
  5. ncbi Germ cell transplantation in pigs--advances and applications
    I Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Soc Reprod Fertil Suppl 62:331-9. 2006
  6. ncbi Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species
    I Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Reprod Fertil Dev 19:732-9. 2007
  7. ncbi Germ cell transplantation and testis tissue xenografting in domestic animals
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Anim Reprod Sci 89:137-45. 2005
  8. ncbi Effect of the GnRH-agonist leuprolide on colonization of recipient testes by donor spermatogonial stem cells after transplantation in mice
    I Dobrinski
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, 19104-6009, USA
    Tissue Cell 33:200-7. 2001
  9. pmc Non-viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation
    W Zeng
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA
    Mol Reprod Dev 79:255-61. 2012
  10. pmc Preservation and transplantation of porcine testis tissue
    W Zeng
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA
    Reprod Fertil Dev 21:489-97. 2009

Research Grants

Collaborators

Detail Information

Publications41

  1. ncbi Transplantation of germ cells and testis tissue for the study and preservation of fertility
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, 145 Myrin Bldg, New Bolton Center, School of Veterinary Medicine University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Soc Reprod Fertil Suppl 65:447-58. 2007
    ..Therefore, transplantation of germ cells or testis tissue are uniquely valuable approaches for the study, preservation and manipulation of male fertility in mammalian species...
  2. ncbi Germ cell transplantation
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Semin Reprod Med 23:257-65. 2005
    ..Therefore, transplantation of germ cells and xenografting of testis tissue represent powerful approaches for the study, preservation, and manipulation of male fertility...
  3. ncbi Advances and applications of germ cell transplantation
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Hum Fertil (Camb) 9:9-14. 2006
    ..Therefore, transplantation of germ cells or xenografting of testis tissue are uniquely valuable approaches for the study, preservation and manipulation of male fertility in mammalian species...
  4. ncbi Male germ cell transplantation
    I Dobrinski
    School of Veterinary Medicine, Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, Kennett Square, PA 19348, USA
    Reprod Domest Anim 43:288-94. 2008
    ..Therefore, transplantation of male germ cells is a uniquely valuable approach for the study, preservation and manipulation of male fertility in animals...
  5. ncbi Germ cell transplantation in pigs--advances and applications
    I Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Soc Reprod Fertil Suppl 62:331-9. 2006
    ..It is expected that germ cell transplantation will then provide a viable alternate approach to generate germ line transgenic pigs...
  6. ncbi Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species
    I Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Rd, Kennett Square, PA 19348, USA
    Reprod Fertil Dev 19:732-9. 2007
    ..Transplantation of germ cells is a uniquely valuable approach for the study, preservation and manipulation of male fertility in mammalian species...
  7. ncbi Germ cell transplantation and testis tissue xenografting in domestic animals
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Anim Reprod Sci 89:137-45. 2005
    ..Therefore, transplantation of germ cells or xenografting of testis tissue are uniquely valuable approaches for the study, preservation and manipulation of male fertility in domestic animals...
  8. ncbi Effect of the GnRH-agonist leuprolide on colonization of recipient testes by donor spermatogonial stem cells after transplantation in mice
    I Dobrinski
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, 19104-6009, USA
    Tissue Cell 33:200-7. 2001
    ..Improved colonization efficiency by treatment with GnRH-agonist can make the technique of spermatogonial transplantation applicable to situations when only low numbers of donor cells are available...
  9. pmc Non-viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation
    W Zeng
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA
    Mol Reprod Dev 79:255-61. 2012
    ..This study provides proof-of-concept that non-viral transfection (nucleofection) of primary goat germ cells followed by germ cell transplantation results in transgene transmission to sperm in recipient goats...
  10. pmc Preservation and transplantation of porcine testis tissue
    W Zeng
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA
    Reprod Fertil Dev 21:489-97. 2009
    ..Therefore, it is important to evaluate germ cell differentiation in vivo in addition to cell viability in vitro when optimising freezing protocols for testis tissue...
  11. ncbi Germ cell development in equine testis tissue xenografted into mice
    R Rathi
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, 19348, USA
    Reproduction 131:1091-8. 2006
    ..In most cases, spermatogenesis arrested at meiosis. The underlying mechanisms of this spermatogenic arrest require further investigation...
  12. ncbi Transplantation of germ cells from rabbits and dogs into mouse testes
    I Dobrinski
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6009, USA
    Biol Reprod 61:1331-9. 1999
    ..These results indicate that fresh and cryopreserved germ cells can colonize the mouse testis but do not differentiate beyond the stage of spermatogonial expansion...
  13. ncbi Transplantation of male germ line stem cells restores fertility in infertile mice
    T Ogawa
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3850 Baltimore Ave, Philadelphia, Pennsylvania 19104, USA
    Nat Med 6:29-34. 2000
    ..Thus, transplantation of spermatogonial stem cells from an infertile donor to a permissive testicular environment can restore fertility and result in progeny with the genetic makeup of the infertile donor male...
  14. ncbi Recent developments in testis tissue xenografting
    Jose R Rodriguez-Sosa
    School of Veterinary Medicine, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA
    Reproduction 138:187-94. 2009
    ..The current paper reviews recent advances that have been obtained with both techniques that represent novel opportunities to explore testis development and spermatogenesis in diverse mammalian species...
  15. pmc Xenografting of adult mammalian testis tissue
    Lucía Arregui
    Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, Kennet Square, PA 19348, USA
    Anim Reprod Sci 106:65-76. 2008
    ..Testis tissue from sub-adult donors survives better as xenograft than tissue from mature adult donors, and complete spermatogenesis can occur albeit with species-specific differences...
  16. ncbi Sperm from neonatal mammalian testes grafted in mice
    Ali Honaramooz
    Male Germ Cell Biology Group, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Nature 418:778-81. 2002
    ..Our results indicate that this approach is applicable to diverse mammalian species...
  17. ncbi Lsh is required for meiotic chromosome synapsis and retrotransposon silencing in female germ cells
    Rabindranath De La Fuente
    Female Germ Cell Biology Group, Department of Clinical Studies, Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, PA 19348, USA
    Nat Cell Biol 8:1448-54. 2006
    ....
  18. ncbi Fertility and germline transmission of donor haplotype following germ cell transplantation in immunocompetent goats
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Biol Reprod 69:1260-4. 2003
    ..In the future, transplantation of genetically modified germ cells may provide a more efficient alternative for production of transgenic domestic animals...
  19. ncbi Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation
    Hannah L Galantino-Homer
    Center for Research on Reproduction and Women s Health, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA
    Mol Reprod Dev 67:487-500. 2004
    ..The mechanism by which heparin uniquely induces intracellular alkalinization in bovine sperm leading to capacitation remains obscure, inviting future investigation...
  20. ncbi Xenografting of degenerate stallion testis onto a mouse host does not rescue the testicular degeneration phenotype
    R M Turner
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, NewBolton Center, University of Pennsylvania School of Veterinary Medicine, PA 19348, USA
    Anim Reprod Sci 89:253-5. 2005
  21. ncbi Xenografting of sheep testis tissue and isolated cells as a model for preservation of genetic material from endangered ungulates
    Lucía Arregui
    Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Reproduction 136:85-93. 2008
    ..Testis tissue de novo morphogenesis from isolated cells could open interesting options to recover germ cells from mature males with impaired spermatogenesis...
  22. ncbi Germ cell fate and seminiferous tubule development in bovine testis xenografts
    Rahul Rathi
    Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, School of Veterinary Medicine, Kennett Square, PA 19348, USA
    Reproduction 130:923-9. 2005
    ..The characterization of spermatogenic efficiency will provide the basis to understand the control of spermatogenesis in testis grafts...
  23. ncbi Accelerated maturation of primate testis by xenografting into mice
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Sciences, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Biol Reprod 70:1500-3. 2004
    ..This approach could potentially be applied to produce fertile sperm from sexually immature individuals of rare or valuable primate species or from prepubertal boys undergoing sterilizing therapy for cancer...
  24. ncbi Germ cell transplantation in pigs
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Biol Reprod 66:21-8. 2002
    ..This study represents a first step toward successful spermatogonial transplantation in a farm animal species...
  25. ncbi Ectopic grafting of mammalian testis tissue into mouse hosts
    Ina Dobrinski
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA
    Methods Mol Biol 450:139-48. 2008
    ....
  26. ncbi Adeno-associated virus (AAV)-mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, 382 W State Road, Kennett Square, PA 19348, USA
    FASEB J 22:374-82. 2008
    ..Equally important, this is also the first report of transgenesis via germ cell transplantation in a nonrodent species, a promising approach to generate transgenic large animal models for biomedical research...
  27. pmc Maturation of testicular tissue from infant monkeys after xenografting into mice
    Rahul Rathi
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, 145 Myrin Building, New Bolton Center, 382 West Street Road, Kennett Square, Pennsylvania 19348, USA
    Endocrinology 149:5288-96. 2008
    ....
  28. pmc Porcine embryos produced after intracytoplasmic sperm injection using xenogeneic pig sperm from neonatal testis tissue grafted in mice
    Ali Honaramooz
    Department of Clinical Sciences, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, PA 19348, USA
    Reprod Fertil Dev 20:802-7. 2008
    ....
  29. ncbi Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus)
    Regina M Turner
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, University of Pennsylvania, School of Veterinary Medicine, 382 West Street Road, Kennett Square, PA 19348, USA
    Reprod Fertil Dev 22:673-83. 2010
    ..These studies demonstrate the usefulness of xenografting for the study of testicular pathology...
  30. ncbi The length of the spermatogenic cycle is conserved in porcine and ovine testis xenografts
    Wenxian Zeng
    Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Building, New Bolton Center, University of Pennsylvania, 382 West St Rd, Kennett Square, PA 19348, USA
    J Androl 27:527-33. 2006
    ..Our results suggest that testis xenografts provide a useful model to study the timing of testicular maturation and spermatogenesis in different mammalian species...
  31. pmc Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, Kennett Square, PA 19348, USA
    J Androl 26:698-705. 2005
    ....
  32. ncbi Effects of 2-hydroxypropyl-beta-cyclodextrin and cholesterol on porcine sperm viability and capacitation status following cold shock or incubation
    Hannah L Galantino-Homer
    Department of Clinical Studies, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA
    Mol Reprod Dev 73:638-50. 2006
    ....
  33. ncbi Building a testis: formation of functional testis tissue after transplantation of isolated porcine (Sus scrofa) testis cells
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Sciences, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Biol Reprod 76:43-7. 2007
    ..This unique in vivo system provides an accessible model for the study of testicular morphogenesis that could be especially useful in nonrodent species...
  34. ncbi Protein gene product 9.5 is a spermatogonia-specific marker in the pig testis: application to enrichment and culture of porcine spermatogonia
    Jinping Luo
    Department of Clinical Studies, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, 19348, USA
    Mol Reprod Dev 73:1531-40. 2006
    ..5. These data provide the basis for future studies aimed at refining conditions of germ cell culture and manipulation prior to germ cell transplantation in pigs...
  35. ncbi A game of cat and mouse: xenografting of testis tissue from domestic kittens results in complete cat spermatogenesis in a mouse host
    Amy K Snedaker
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA
    J Androl 25:926-30. 2004
    ..Therefore, sperm production in a mouse host can provide an alternative for germ line preservation from immature felids where sperm cryopreservation is not an option...
  36. ncbi Comparison of global gene expression between porcine testis tissue xenografts and porcine testis in situ
    Wenxian Zeng
    Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
    Mol Reprod Dev 74:674-9. 2007
    ..These findings support the hypothesis that testis tissue xenografts can provide a representative model to study mammalian spermatogenesis...
  37. pmc Asymmetric distribution of UCH-L1 in spermatogonia is associated with maintenance and differentiation of spermatogonial stem cells
    Jinping Luo
    Department of Clinical Studies, Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA, USA
    J Cell Physiol 220:460-8. 2009
    ..These findings for the first time provide direct evidence for existence of asymmetric division during SSCs self-renewal and differentiation in mammalian spermatogenesis...
  38. ncbi Germ cell transplantation in goats
    Ali Honaramooz
    Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA
    Mol Reprod Dev 64:422-8. 2003
    ..This study represents the first report of germ cell transplantation in goats...
  39. ncbi Successful transplantation of bovine testicular cells to heterologous recipients
    Muren Herrid
    CSIRO Livestock Industries, Food Futures National Research Flagship, Locked Bag 1, Post Office, Armidale, New South Wales 2350, Australia
    Reproduction 132:617-24. 2006
    ..These results indicate that allogeneic transplantation of testicular cells can occur between Bos taurus and Bos indicus cattle. Further studies will investigate functionality of transferred testicular cells...
  40. ncbi Progeny from sperm obtained after ectopic grafting of neonatal mouse testes
    Stefan Schlatt
    Institute of Reproductive Medicine, 48149 Munster, Germany
    Biol Reprod 68:2331-5. 2003
    ..The ability to generate sperm from the germ line ex vivo also paves the way for the development of new strategies for preserving fertility in boys undergoing cancer therapy...
  41. ncbi Recipient preparation and mixed germ cell isolation for spermatogonial stem cell transplantation in domestic cats
    Yeunhee Kim
    The James A Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Androl 27:248-56. 2006
    ..The success of these protocols provides a foundation to perform spermatogonial stem cell transplantation in the domestic cat...

Research Grants2

  1. IDENTIFICATION OF STEM CELL MARKERS IN MAMMALIAN TESTES
    Ina Dobrinski; Fiscal Year: 2002
    ..abstract_text> ..
  2. Transgenic Goats Produced by Germ Cell Transplantation
    Ina Dobrinski; Fiscal Year: 2006
    ..unreadable] [unreadable]..