JOHN DONELSON

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi request reprint Multiple mechanisms of immune evasion by African trypanosomes
    J E Donelson
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Mol Biochem Parasitol 91:51-66. 1998
  2. ncbi request reprint A novel protein targeting domain directs proteins to the anterior cytoplasmic face of the flagellar pocket in African trypanosomes
    K L Hill
    Department of Biochemistry, University of Iowa, IA 52242, USA
    J Cell Sci 112:3091-101. 1999
  3. ncbi request reprint Antigenic variation and the African trypanosome genome
    John E Donelson
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Acta Trop 85:391-404. 2003
  4. ncbi request reprint Leishmania chagasi: the alpha-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state
    Jay E Purdy
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 110:102-7. 2005
  5. pmc Differential expression of a protease gene family in African trypanosomes
    Jared R Helm
    Department of Biochemistry, University of Iowa, 4 339 Bowen Science Bldg, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 163:8-18. 2009
  6. ncbi request reprint Comparison of the post-transcriptional regulation of the mRNAs for the surface proteins PSA (GP46) and MSP (GP63) of Leishmania chagasi
    Karen S Myung
    Department of Biochemistry, University of Iowa and the Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
    J Biol Chem 277:16489-97. 2002
  7. ncbi request reprint Trypanosoma brucei FLA1 is required for flagellum attachment and cytokinesis
    Douglas J LaCount
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:17580-8. 2002
  8. ncbi request reprint Internal and surface subpopulations of the major surface protease (MSP) of Leishmania chagasi
    Chaoqun Yao
    VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 139:173-83. 2005
  9. ncbi request reprint Regulation of genes encoding the major surface protease of Leishmania chagasi via mRNA stability
    Jay E Purdy
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 142:88-97. 2005
  10. pmc Leishmania chagasi: a tetracycline-inducible cell line driven by T7 RNA polymerase
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 116:205-13. 2007

Research Grants

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Multiple mechanisms of immune evasion by African trypanosomes
    J E Donelson
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Mol Biochem Parasitol 91:51-66. 1998
    ....
  2. ncbi request reprint A novel protein targeting domain directs proteins to the anterior cytoplasmic face of the flagellar pocket in African trypanosomes
    K L Hill
    Department of Biochemistry, University of Iowa, IA 52242, USA
    J Cell Sci 112:3091-101. 1999
    ..The finding that TLTF-GFP is restricted to the anterior, cytoplasmic face of the flagellar pocket membrane, suggests that there is structural heterogeneity in the membrane of this organelle...
  3. ncbi request reprint Antigenic variation and the African trypanosome genome
    John E Donelson
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Acta Trop 85:391-404. 2003
    ..Progress in sequencing the African trypanosome genome has led to additional insights on the organization of genes within both groups of ESs that may ultimately suggest better ways to control or eliminate this deadly pathogen...
  4. ncbi request reprint Leishmania chagasi: the alpha-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state
    Jay E Purdy
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 110:102-7. 2005
    ..These data suggest that the alpha-TUB intercoding region is an excellent control for the study of the regulation of other differentially expressed genes...
  5. pmc Differential expression of a protease gene family in African trypanosomes
    Jared R Helm
    Department of Biochemistry, University of Iowa, 4 339 Bowen Science Bldg, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 163:8-18. 2009
    ..Thus, the 23-bp element of this protease gene affects both the level of RNA and its translation...
  6. ncbi request reprint Comparison of the post-transcriptional regulation of the mRNAs for the surface proteins PSA (GP46) and MSP (GP63) of Leishmania chagasi
    Karen S Myung
    Department of Biochemistry, University of Iowa and the Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
    J Biol Chem 277:16489-97. 2002
    ....
  7. ncbi request reprint Trypanosoma brucei FLA1 is required for flagellum attachment and cytokinesis
    Douglas J LaCount
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:17580-8. 2002
    ..These experiments demonstrate that the FLA proteins are essential and suggest that in procyclic T. brucei, the FLA1 protein has separable functions in flagellar attachment and cytokinesis...
  8. ncbi request reprint Internal and surface subpopulations of the major surface protease (MSP) of Leishmania chagasi
    Chaoqun Yao
    VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 139:173-83. 2005
    ..These data are consistent with the hypothesis that the major mechanism regulating MSP abundance is the rate of loss of surface-localized MSP from the promastigote surface, and that internally localized MSP is very stable...
  9. ncbi request reprint Regulation of genes encoding the major surface protease of Leishmania chagasi via mRNA stability
    Jay E Purdy
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 142:88-97. 2005
    ..Hence, we have identified a 202-nucleotide region within the MSPL 3'UTR that is in part responsible for cycloheximide induction. We hypothesize that this region may interact with labile regulatory protein factor(s)...
  10. pmc Leishmania chagasi: a tetracycline-inducible cell line driven by T7 RNA polymerase
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 116:205-13. 2007
    ..that allows the exogenous T7RNAP-driven gene expression to be tetracycline-inducible; and may provide a useful tool for addressing protein function by manipulating expression levels of Leishmania endogenous genes...
  11. pmc Internal and surface-localized major surface proteases of Leishmania spp. and their differential release from promastigotes
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Eukaryot Cell 6:1905-12. 2007
    ..We present a model in which these different MSP pools are released under distinct life cycle-specific conditions...
  12. pmc Leishmania chagasi: homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, IA 52242, USA
    Exp Parasitol 118:129-33. 2008
    ..These metacyclic L. chagasi expressed both the virulence-associated 59-kDa, and the constitutively expressed 63-kDa, isoforms of MSP...
  13. pmc The major surface protease (MSP or GP63) in the intracellular amastigote stage of Leishmania chagasi
    Chia Hung Christine Hsiao
    Molecular Biology Program, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 157:148-59. 2008
    ..We hypothesize that MSP plays different roles in the extracellular versus intracellular forms of Leishmania spp...
  14. ncbi request reprint Multiple products of the Leishmania chagasi major surface protease (MSP or GP63) gene family
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, 300L, EMRB, Newton Road, Iowa City, IA 52242, USA Chaoqun
    Mol Biochem Parasitol 135:171-83. 2004
    ..These data demonstrate that the products of individual MSP genes are differentially expressed during Leishmania development. We hypothesize they may play different roles during parasite migration through its two hosts...
  15. ncbi request reprint The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function
    Chaoqun Yao
    VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 132:1-16. 2003
    ..The MSPs exquisitely demonstrate the multiple levels of post-transcriptional gene regulation that occur in Leishmania sp. and other trypanosomatid protozoa...
  16. ncbi request reprint Expression and function of the Trypanosoma brucei major surface protease (GP63) genes
    Douglas J LaCount
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    J Biol Chem 278:24658-64. 2003
    ..This finding is consistent with previous pharmacological studies showing that peptidomimetic collagenase inhibitors block release of transgenic VSG from procyclic trypanosomes and are toxic for bloodstream but not procyclic organisms...
  17. pmc Leishmania infantum chagasi: a genome-based approach to identification of excreted/secreted proteins
    Sruti DebRoy
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 126:582-91. 2010
    ..We found both amastigote-specific and promastigote-specific released proteins. The ES proteins of Lic are candidates for future studies of parasite virulence determinants and host protective immunity...
  18. pmc A function for a specific zinc metalloprotease of African trypanosomes
    Paul M Grandgenett
    Interdepartmental Genetics Program, University of Iowa, Iowa City, Iowa, USA
    PLoS Pathog 3:1432-45. 2007
    ..Inhibitors of cysteine proteases did not affect this result. These findings demonstrate that removal of 60% of the VSG during differentiation from bloodstream to procyclic form is due to the synergistic activities of GPI-PLC and TbMSP-B...
  19. ncbi request reprint Trypanosoma brucei: a first-generation CRE-loxP site-specific recombination system
    Brian Barrett
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Exp Parasitol 106:37-44. 2004
    ..brucei genome when it is not needed before the full range of CRE-loxP applications currently used in mice can be exploited in African trypanosomes...
  20. pmc Analysis of expressed sequence tags from the four main developmental stages of Trypanosoma congolense
    Jared R Helm
    Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 168:34-42. 2009
    ..The T. congolense EST databases are available at: http://www.sanger.ac.uk/Projects/T_congolense/EST_index.shtml. The sequence data have been submitted to EMBL under the following accession numbers: FN263376-FN292969...
  21. pmc Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes
    Nathan R Hutchings
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    J Cell Biol 156:867-77. 2002
    ..These results indicate that trypanin functions as a cytoskeletal linker protein and offer insights into the mechanisms of flagellum-based cell motility...
  22. pmc Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei
    Jared R Helm
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 159:134-7. 2008
    ..These alternative SL addition sites may be aberrant or they might be utilized to expand the number of gene products from individual genes. Future experiments on endogenous genes will be necessary to examine the latter possibility...
  23. ncbi request reprint Biosynthesis of the major surface protease GP63 of Leishmania chagasi
    Chaoqun Yao
    Department of Internal Medicine, University of Iowa, SW34 GH, 200 Hawkins Dr, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 121:119-28. 2002
    ..The current findings add yet another level of complexity to post-transcriptionally regulated expression of an abundant surface molecule in a trypanosomatid protozoan...
  24. pmc Leishmania chagasi T-cell antigens identified through a double library screen
    Daniella R A Martins
    Department of Internal Medicine, University of Iowa, SW34 GH, 200 Hawkins Drive, Iowa City, IA 52242, USA
    Infect Immun 74:6940-8. 2006
    ..Humans naturally infected with L. chagasi mounted both cellular and antibody responses to these proteins. Preparations containing multiple antigens may be optimal for immunodiagnosis and protective vaccines...
  25. ncbi request reprint Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi
    Wanderson D DaRocha
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Caixa Postal 486, Belo Horizonte, Brazil
    Mol Biochem Parasitol 133:175-86. 2004
    ..cruzi lacks one or more components necessary for the RNAi pathway and that these components will need to be engineered into T. cruzi, or compensated for, before RNAi can be used to study gene function in this organism...
  26. ncbi request reprint Surface glycoprotein PSA (GP46) expression during short- and long-term culture of Leishmania chagasi
    Jeffrey K Beetham
    Department of Veterinary Pathology, Iowa State University, 2714 Veterinary Medicine, Ames, IA 50011, USA
    Mol Biochem Parasitol 131:109-17. 2003
    ....
  27. ncbi request reprint Genetic complementation of Leishmania deficient in PSA (GP46) restores their resistance to lysis by complement
    Leslie M Lincoln
    Department of Entomology, Iowa State University, Ames 50011, USA
    Mol Biochem Parasitol 137:185-9. 2004
  28. pmc The sequence and analysis of Trypanosoma brucei chromosome II
    Najib M A El-Sayed
    The Institute for Genomic Research, Rockville, MD 20850, USA
    Nucleic Acids Res 31:4856-63. 2003
    ....
  29. ncbi request reprint Tetracycline-regulated RNA interference in Trypanosoma congolense
    Noboru Inoue
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 120:309-13. 2002
  30. ncbi request reprint Trypanosoma cruzi: RNA structure and post-transcriptional control of tubulin gene expression
    Daniella C Bartholomeu
    Departamento de Bioquimica e Imunologia, ICB, da Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270 010, Brazil
    Exp Parasitol 102:123-33. 2002
    ....
  31. ncbi request reprint Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
    ..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
  32. pmc A new, expressed multigene family containing a hot spot for insertion of retroelements is associated with polymorphic subtelomeric regions of Trypanosoma brucei
    Frederic Bringaud
    Laboratoire de Parasitologie Moleculaire, Université Victor Segalen Bordeaux II, UMR 5016 CNRS, 33076 Bordeaux, France
    Eukaryot Cell 1:137-51. 2002
    ..brucei strain TREU927/4 GUTat10.1. RHS genes comprise the remaining sequence of the size-polymorphic "repetitive region" described for T. brucei chromosome I, and a homologous gene family is present in the Trypanosoma cruzi genome...
  33. ncbi request reprint The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  34. ncbi request reprint Characterization of cDNA clones encoding ribonucleoprotein antigens expressed in Trypanosoma cruzi amastigotes
    Wanderson D DaRocha
    Departamento de Bioquimica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    Parasitol Res 88:292-300. 2002
    ..Patient humoral responses against the recombinant proteins encoded by these cDNAs were evaluated in anticipation that they may constitute potential new targets for serodiagnostic assays...
  35. ncbi request reprint Procyclic Trypanosoma brucei expresses separate sialidase and trans-sialidase enzymes on its surface membrane
    Georgina N Montagna
    Instituto de Investigaciones Biotecnologicas, Universidad de General San Martín, 1650 San Martin, Pcia de Buenos Aires, Argentina
    J Biol Chem 281:33949-58. 2006
    ..These results suggest that African trypanosomes could regulate the amount of critical sialic acid residues on their surface by modulating differential expression of each of these enzymes...

Research Grants3

  1. AMASTIN/TUZIN GENE CLUSTER OF TRYPANOSOMA CRUZI
    JOHN DONELSON; Fiscal Year: 2001
    ..Finally, we will assess whether amastin and/or tuzin can serve a target antigens for an improved diagnostic test for T. cruzi infection. ..
  2. GP46 GENES AND PROTEIN EXPRESSION IN LEISHMANIA CHAGASI
    JOHN DONELSON; Fiscal Year: 2002
    ....
  3. Zinc metalloprotease families in Trypansoma brucei
    JOHN DONELSON; Fiscal Year: 2009
    ....