Feng Guo

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8
    Rachel Senturia
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, USA
    PLoS ONE 7:e39688. 2012
  2. pmc Ferric, not ferrous, heme activates RNA-binding protein DGCR8 for primary microRNA processing
    Ian Barr
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:1919-24. 2012
  3. pmc Structure of the dimerization domain of DiGeorge critical region 8
    Rachel Senturia
    Department of Biological Chemistry in David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Protein Sci 19:1354-65. 2010
  4. pmc DiGeorge critical region 8 (DGCR8) is a double-cysteine-ligated heme protein
    Ian Barr
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 286:16716-25. 2011
  5. pmc Processing of microRNA primary transcripts requires heme in mammalian cells
    Sara H Weitz
    Interdepartmental Program in Molecular, Cellular, and Integrative Physiology, Department of Biological Chemistry, David Geffen School of Medicine, Department of Chemistry and Biochemistry, and Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 111:1861-6. 2014
  6. pmc Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Ming Gong
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Protein Sci 21:797-808. 2012
  7. ncbi request reprint Primary microRNA processing assay reconstituted using recombinant Drosha and DGCR8
    Ian Barr
    Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA
    Methods Mol Biol 1095:73-86. 2014
  8. pmc DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures
    Michael Faller
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    RNA 16:1570-83. 2010
  9. pmc Site-directed spin labeling electron paramagnetic resonance study of the ORF1 protein from a mouse L1 retrotransposon
    Kurt Januszyk
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1570, USA
    Protein Sci 20:1231-43. 2011
  10. pmc Identification of a cis-acting element that localizes mRNA to synapses
    Elliott J Meer
    Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:4639-44. 2012

Collaborators

  • Joseph A Loo
  • Christopher Lee
  • David Eisenberg
  • Christian Altenbach
  • Qi Wang
  • Ian Barr
  • Rachel Senturia
  • Michael Faller
  • Yanqiu Chen
  • Sara H Weitz
  • Ming Gong
  • Jen Quick-Cleveland
  • Elliott J Meer
  • Aaron T Smith
  • Brooke D Scheidemantle
  • Judith N Burstyn
  • Kurt Januszyk
  • Robert T Clubb
  • Duilio Cascio
  • Linda Miallau
  • Sheng Yin
  • Michio Matsunaga
  • Grant Shoffner
  • Shimon Weiss
  • Jose P Jacob
  • Arthur Laganowsky
  • Kelsey C Martin
  • Matthew Ulgherait
  • Dan Ohtan Wang
  • Sangmok Kim
  • Mark R Fleissner
  • Sandra L Martin
  • Fa Kuen Shieh
  • Wayne L Hubbell
  • Lara Atchabahian
  • Daniel Hwang
  • Ivo Atanasov
  • Daniel Toso
  • Z Hong Zhou
  • Michael R Sawaya
  • Janet Chiang
  • Mark Arbing

Detail Information

Publications15

  1. pmc Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8
    Rachel Senturia
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, USA
    PLoS ONE 7:e39688. 2012
    ..The structure is very similar to that of human DGCR8. Our results indicate that dimerization and heme binding are evolutionarily conserved properties of DGCR8 homologues not only in vertebrates, but also in at least some invertebrates...
  2. pmc Ferric, not ferrous, heme activates RNA-binding protein DGCR8 for primary microRNA processing
    Ian Barr
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:1919-24. 2012
    ....
  3. pmc Structure of the dimerization domain of DiGeorge critical region 8
    Rachel Senturia
    Department of Biological Chemistry in David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Protein Sci 19:1354-65. 2010
    ..Our study provides structural and biochemical bases for understanding how dimerization and heme binding of DGCR8 may contribute to regulation of miRNA biogenesis...
  4. pmc DiGeorge critical region 8 (DGCR8) is a double-cysteine-ligated heme protein
    Ian Barr
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 286:16716-25. 2011
    ..We further show that native DGCR8 binds heme when expressed in eukaryotic cells. This study provides a chemical basis for understanding the function of the DGCR8-heme interaction in microRNA maturation...
  5. pmc Processing of microRNA primary transcripts requires heme in mammalian cells
    Sara H Weitz
    Interdepartmental Program in Molecular, Cellular, and Integrative Physiology, Department of Biological Chemistry, David Geffen School of Medicine, Department of Chemistry and Biochemistry, and Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 111:1861-6. 2014
    ..Our study suggests that abnormal heme biosynthesis and degradation may contribute to diseases via miRNA-mediated gene regulation networks. ..
  6. pmc Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Ming Gong
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Protein Sci 21:797-808. 2012
    ..We suggest that both the Drosha and Dicer cleavage steps of the miRNA maturation pathway may be inhibited in apoptosis and other biological processes where caspases are activated...
  7. ncbi request reprint Primary microRNA processing assay reconstituted using recombinant Drosha and DGCR8
    Ian Barr
    Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA
    Methods Mol Biol 1095:73-86. 2014
    ..In particular, we present the procedures for expressing and purifying DGCR8 as an Fe(III) heme-bound dimer, the most active form of this protein, and for estimating its heme content. ..
  8. pmc DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures
    Michael Faller
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    RNA 16:1570-83. 2010
    ..Our study provides a molecular basis for recognition of pri-miRNAs by DGCR8. We further propose that the higher-order structures of the DGCR8-pri-miRNA complexes trigger the cleavage of pri-miRNAs by Drosha...
  9. pmc Site-directed spin labeling electron paramagnetic resonance study of the ORF1 protein from a mouse L1 retrotransposon
    Kurt Januszyk
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1570, USA
    Protein Sci 20:1231-43. 2011
    ..We propose that in the context of the full-length trimeric protein these distinct surfaces are positioned adjacent to one another providing a continuous surface that may interact with nucleic acids...
  10. pmc Identification of a cis-acting element that localizes mRNA to synapses
    Elliott J Meer
    Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:4639-44. 2012
    ..We have identified a 66-nt element in the 5' UTR of sensorin that is necessary and sufficient for synaptic mRNA localization. Mutational and chemical probing analyses are consistent with a role for secondary structure in this process...
  11. pmc The DGCR8 RNA-binding heme domain recognizes primary microRNAs by clamping the hairpin
    Jen Quick-Cleveland
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Cell Rep 7:1994-2005. 2014
    ..Our study reveals a unique protein-RNA interaction central to pri-miRNA recognition. We propose a unifying model in which two DGCR8 dimers clamp a pri-miRNA hairpin using their Rheds...
  12. pmc Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis
    Linda Miallau
    UCLA DOE Institute of Genomics and Proteomics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095 1570, USA
    J Biol Chem 284:276-83. 2009
    ..Furthermore, analysis of the interactions in the binding of the antitoxin to the toxin suggest that exquisite control is required to protect the bacteria cell from toxic VapC-5...
  13. pmc MicroRNA biogenesis: there's more than one way to skin a cat
    Michael Faller
    Department of Biological Chemistry, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Biochim Biophys Acta 1779:663-7. 2008
    ..The relationship between the diversity in miRNA biogenesis and the apparently rapid evolution of miRNA genes and functions is discussed...
  14. pmc Evidence of a novel RNA secondary structure in the coding region of HIV-1 pol gene
    Qi Wang
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    RNA 14:2478-88. 2008
    ..Finally, this structure was validated experimentally using selective 2'-hydroxyl acylation and primer extension (SHAPE). Discovery of this novel secondary structure suggests many directions for further functional investigation...
  15. ncbi request reprint Heme is involved in microRNA processing
    Michael Faller
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles UCLA, Los Angeles, California 90095, USA
    Nat Struct Mol Biol 14:23-9. 2007
    ..This putative autoinhibition is overcome by heme. Our finding that heme is involved in pri-miRNA processing suggests that the gene-regulation network of miRNAs and signal-transduction pathways involving heme might be connected...

Research Grants4

  1. Molecular recognition and regulation in microRNA processing by the DGCR8 protein
    Feng Guo; Fiscal Year: 2007
    ..Our discovery that heme may be involved in regulating the processing of microRNAs suggest that they can be used as therapeutic agents for microRNA-related diseases. ..
  2. Molecular recognition and regulation in microRNA processing by the DGCR8 protein
    Feng Guo; Fiscal Year: 2009
    ..Our discovery that heme may be involved in regulating the processing of microRNAs suggest that they can be used as therapeutic agents for microRNA-related diseases. ..
  3. Molecular recognition and regulation in microRNA processing by the DGCR8 protein
    Feng Guo; Fiscal Year: 2010
    ..Our discovery that heme may be involved in regulating the processing of microRNAs suggest that they can be used as therapeutic agents for microRNA-related diseases. ..