P D Nagy

Summary

Affiliation: University of Kentucky
Country: USA

Publications

  1. ncbi request reprint Use of double-stranded RNA templates by the tombusvirus replicase in vitro: Implications for the mechanism of plus-strand initiation
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 352:110-20. 2006
  2. pmc Sterol-binding by the tombusviral replication proteins is essential for replication in yeast and plants
    Kai Xu
    Department of Plant Pathology University of Kentucky, Lexington, KY 40546, USA
    J Virol . 2017
  3. ncbi request reprint Tombusvirus-Host Interactions: Co-Opted Evolutionarily Conserved Host Factors Take Center Court
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546 email
    Annu Rev Virol 3:491-515. 2016
  4. pmc Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Viruses 8:68. 2016
  5. pmc Viral sensing of the subcellular environment regulates the assembly of new viral replicase complexes during the course of infection
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    J Virol 89:5196-9. 2015
  6. doi request reprint How yeast can be used as a genetic platform to explore virus-host interactions: from 'omics' to functional studies
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY, USA Electronic address
    Trends Microbiol 22:309-16. 2014
  7. pmc Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis
    Zsuzsanna Sasvari
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 7:e1002438. 2011
  8. pmc Inhibition of RNA recruitment and replication of an RNA virus by acridine derivatives with known anti-prion activities
    Zsuzsanna Sasvari
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS ONE 4:e7376. 2009
  9. pmc A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus
    Daniel Barajas
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    PLoS Pathog 5:e1000705. 2009
  10. pmc The TPR domain in the host Cyp40-like cyclophilin binds to the viral replication protein and inhibits the assembly of the tombusviral replicase
    Jing Yi Lin
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 8:e1002491. 2012

Collaborators

  • Kai Xu
  • Jun Qin
  • Jing Yi Lin
  • K Andrew White
  • GUANGXIANG G LUO
  • Wei Xu
  • Tadas Panavas
  • Judit Pogany
  • Chi Ping Cheng
  • K S Rajendran
  • Elena Serviene
  • Zivile Panaviene
  • Hannah M Jaag
  • Zsuzsanna Sasvari
  • T Panavas
  • Kunj B Pathak
  • Nikolay Kovalev
  • Daniel Barajas
  • Jozsef Stork
  • Zhenghe Li
  • Natalia Shapka
  • Yi Jiang
  • J Pogany
  • Monika Sharma
  • Magdalena Jonczyk
  • Chingkai Chuang
  • Saulius Serva
  • Tyng Shyan Huang
  • Terri Goss Kinzy
  • Robert Yung Liang Wang
  • Jannine Baker
  • Sandra Monkewich
  • KaJohn Boonrod
  • Marc R Fabian
  • C P Cheng
  • Qiasheng Lu
  • Lara Izotova
  • Mark E Schmitt
  • Steven Tupman
  • Anthony M Esposito
  • Stephane Bach
  • Marc Blondel
  • David A Herbst
  • Jozsef Gal
  • Hong Na
  • Han Xin Lin
  • Debashish Ray
  • Jeremy Brasher
  • Cecily M Hawkins
  • Bencharong Phuangrat
  • Olena A Chernysheva
  • Danuta Galetzka
  • Udo Conrad
  • Gabi Krczal
  • Jannine M Baker
  • Z Panaviene

Detail Information

Publications69

  1. ncbi request reprint Use of double-stranded RNA templates by the tombusvirus replicase in vitro: Implications for the mechanism of plus-strand initiation
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 352:110-20. 2006
    ....
  2. pmc Sterol-binding by the tombusviral replication proteins is essential for replication in yeast and plants
    Kai Xu
    Department of Plant Pathology University of Kentucky, Lexington, KY 40546, USA
    J Virol . 2017
    ..Altogether, the obtained and the previously published results support the local enrichment of sterols around the viral replication proteins that is critical for TBSV replication...
  3. ncbi request reprint Tombusvirus-Host Interactions: Co-Opted Evolutionarily Conserved Host Factors Take Center Court
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546 email
    Annu Rev Virol 3:491-515. 2016
    ..Altogether, these advances in our understanding of tombusvirus-host interactions are broadly applicable to many other viruses, which also usurp conserved host factors for various viral processes...
  4. pmc Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Viruses 8:68. 2016
    ..Remarkably, all the known steps in TBSV replication are dependent on cellular lipids and co-opted membranes. ..
  5. pmc Viral sensing of the subcellular environment regulates the assembly of new viral replicase complexes during the course of infection
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    J Virol 89:5196-9. 2015
    ..These regulatory mechanisms might explain how tombusviruses could adjust the efficiency of RNA replication and new VRC assembly to the limiting resources of the host cells during infections. ..
  6. doi request reprint How yeast can be used as a genetic platform to explore virus-host interactions: from 'omics' to functional studies
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY, USA Electronic address
    Trends Microbiol 22:309-16. 2014
    ..The knowledge gained on host factors could lead to novel specific or broad-range resistance and antiviral tools against viruses. ..
  7. pmc Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis
    Zsuzsanna Sasvari
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 7:e1002438. 2011
    ..These roles for translation factors during TBSV replication are separate from their canonical roles in host and viral protein translation...
  8. pmc Inhibition of RNA recruitment and replication of an RNA virus by acridine derivatives with known anti-prion activities
    Zsuzsanna Sasvari
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS ONE 4:e7376. 2009
    ....
  9. pmc A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus
    Daniel Barajas
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    PLoS Pathog 5:e1000705. 2009
    ....
  10. pmc The TPR domain in the host Cyp40-like cyclophilin binds to the viral replication protein and inhibits the assembly of the tombusviral replicase
    Jing Yi Lin
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 8:e1002491. 2012
    ..Overall, the current work revealed a role for Cyp40-like proteins and their TPR domains as regulators of RNA virus replication...
  11. pmc The combined effect of environmental and host factors on the emergence of viral RNA recombinants
    Hannah M Jaag
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, Kentucky, United States of America
    PLoS Pathog 6:e1001156. 2010
    ..Thus, host factors in combination with environmental factors likely affect virus evolution and adaptation...
  12. pmc Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis
    Zhenghe Li
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 6:e1001175. 2010
    ..These roles for eEF1A are separate from its canonical role in host and viral protein translation, emphasizing critical functions for this abundant cellular protein during TBSV replication...
  13. pmc The expanding functions of cellular helicases: the tombusvirus RNA replication enhancer co-opts the plant eIF4AIII-like AtRH2 and the DDX5-like AtRH5 DEAD-box RNA helicases to promote viral asymmetric RNA replication
    Nikolay Kovalev
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 10:e1004051. 2014
    ....
  14. pmc Inactivation of the host lipin gene accelerates RNA virus replication through viral exploitation of the expanded endoplasmic reticulum membrane
    Chingkai Chuang
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 10:e1003944. 2014
    ..Over-expression of AtPah2p also inhibited the ER-based replication of another plant RNA virus, suggesting that the role of lipins in RNA virus replication might include several more eukaryotic viruses. ..
  15. pmc A Co-Opted DEAD-Box RNA helicase enhances tombusvirus plus-strand synthesis
    Nikolay Kovalev
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 8:e1002537. 2012
    ..Thus, two small RNA viruses, which do not code for their own helicases, seems to recruit a host RNA helicase to aid their replication in infected cells...
  16. doi request reprint Host factors with regulatory roles in tombusvirus replication
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Curr Opin Virol 2:691-8. 2012
    ..This facilitates tight spatial and temporal organization of viral replication in infected cells...
  17. doi request reprint The dependence of viral RNA replication on co-opted host factors
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, Kentucky 40546, USA
    Nat Rev Microbiol 10:137-49. 2011
    ..This Review presents the recent progress that has been made in understanding the role of co-opted host proteins and membranes during (+)RNA virus replication, and discusses common themes employed by different viruses...
  18. doi request reprint Emerging picture of host chaperone and cyclophilin roles in RNA virus replication
    P D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 411:374-82. 2011
    ..This review also discusses the emerging roles of cyclophilins, which are peptidyl-prolyl isomerases with chaperone functions, in replication of selected (+)RNA viruses...
  19. doi request reprint Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Adv Virus Res 76:123-77. 2010
    ....
  20. doi request reprint Yeast as a model host to explore plant virus-host interactions
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    Annu Rev Phytopathol 46:217-42. 2008
    ....
  21. ncbi request reprint Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 344:211-20. 2006
    ..The knowledge obtained will likely stimulate development of new antiviral methods as well as other approaches that could make tombusviruses useful tools in biotechnological applications...
  22. pmc Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication
    Judit Pogany
    University of Kentucky, Department of Plant Pathology, 201F Plant Science Bldg, Lexington, KY 40546, USA
    J Virol 79:4859-69. 2005
    ..Since all genera in Tombusviridae encode comparable replicase proteins, these results may be relevant to other members of this large virus family...
  23. pmc The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination
    Natalia Shapka
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 78:2288-300. 2004
    ..In summary, evidence is presented that a tombusvirus can use the recombination signal of BMV. This supports the idea that common AU-rich recombination signals might promote interviral recombination between unrelated viruses...
  24. pmc Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro
    Kunj B Pathak
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    J Virol 86:156-71. 2012
    ..Collectively, this work has revealed new functions for tombusvirus cis-acting RNA elements and provided insights into the pioneering round of minus-strand synthesis...
  25. ncbi request reprint Internal initiation by the cucumber necrosis virus RNA-dependent RNA polymerase is facilitated by promoter-like sequences
    T Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    Virology 296:275-87. 2002
    ..This may explain why tombusviruses are frequently associated with DI RNAs...
  26. ncbi request reprint Partial purification and characterization of Cucumber necrosis virus and Tomato bushy stunt virus RNA-dependent RNA polymerases: similarities and differences in template usage between tombusvirus and carmovirus RNA-dependent RNA polymerases
    P D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, 40546, USA
    Virology 276:279-88. 2000
    ..1999, EMBO J. 18, 5653-5665) does not stimulate tombusvirus RdRp activity. Taken together, these results suggest that template usage by the tombusvirus and carmovirus RdRps are similar, but not identical...
  27. ncbi request reprint Enhancement of RNA synthesis by promoter duplication in tombusviruses
    T Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 310:118-29. 2003
    ....
  28. ncbi request reprint Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase
    T Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    Virology 296:263-74. 2002
    ..Taken together, these results demonstrate that promoter recognition by the tombusvirus RdRp requires only short sequences present at the 3' end of templates...
  29. ncbi request reprint Mechanism of DI RNA formation in tombusviruses: dissecting the requirement for primer extension by the tombusvirus RNA dependent RNA polymerase in vitro
    C P Cheng
    Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
    Virology 304:460-73. 2002
    ..In addition, these findings support a model that predicts a higher frequency of replicase jumping, i.e., recombination/deletion events, during plus-strand synthesis than during minus-strand synthesis...
  30. pmc The RNA replication enhancer element of tombusviruses contains two interchangeable hairpins that are functional during plus-strand synthesis
    T Panavas
    Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
    J Virol 77:258-69. 2003
    ..Ray and K. A. White, Virology 256:162-171, 1999) and they firmly establish the presence of a replication enhancer on the minus-stranded RNA of tombusviruses...
  31. ncbi request reprint In vivo and in vitro characterization of an RNA replication enhancer in a satellite RNA associated with turnip crinkle virus
    P D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    Virology 288:315-24. 2001
    ....
  32. pmc Comparison of turnip crinkle virus RNA-dependent RNA polymerase preparations expressed in Escherichia coli or derived from infected plants
    K S Rajendran
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 76:1707-17. 2002
    ..Further comparison of the single-component recombinant TCV RdRp and the multicomponent plant TCV RdRp will help dissect the functions of various components of the TCV replicase...
  33. pmc Direct inhibition of tombusvirus plus-strand RNA synthesis by a dominant negative mutant of a host metabolic enzyme, glyceraldehyde-3-phosphate dehydrogenase, in yeast and plants
    Tyng Shyan Huang
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    J Virol 85:9090-102. 2011
    ..Overall, the obtained data support the model that the co-opted GAPDH plays a direct role in TBSV replication by stimulating plus-strand synthesis by the viral replicase...
  34. pmc Role of RNase MRP in viral RNA degradation and RNA recombination
    Hannah M Jaag
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546 0091, USA
    J Virol 85:243-53. 2011
    ..Altogether, this work suggests a role for the host endoribonuclease RNase MRP in viral RNA degradation and recombination...
  35. pmc Inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants
    Monika Sharma
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 84:2270-81. 2010
    ..benthamiana reduced TBSV RNA accumulation but had a lesser inhibitory effect on the unrelated Tobacco mosaic virus, suggesting that various viruses show different levels of dependence on sterol biosynthesis for their replication...
  36. ncbi request reprint Interaction between the replicase proteins of Tomato bushy stunt virus in vitro and in vivo
    K S Rajendran
    Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
    Virology 326:250-61. 2004
    ....
  37. ncbi request reprint Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: implication for modular evolution of RNA viruses
    Chi Ping Cheng
    Department of Plant Pathology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40546, USA
    Virology 341:107-21. 2005
    ..Based on these observations, we propose that REN elements could facilitate intervirus recombination and post-recombinational amplification of new recombinant viruses...
  38. pmc Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase
    Zivile Panaviene
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546 0312, USA
    J Virol 79:10608-18. 2005
    ..Altogether, the role of multiple RNA elements in tombusvirus replicase assembly could be an important factor to ensure fidelity of template selection during replication...
  39. pmc Mechanism of stimulation of plus-strand synthesis by an RNA replication enhancer in a tombusvirus
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, 40546, USA
    J Virol 79:9777-85. 2005
    ..We propose that REN is important for asymmetrical viral RNA replication that leads to more abundant plus-strand RNA progeny than the minus-strand intermediate, a hallmark of replication of plus-strand RNA viruses...
  40. ncbi request reprint The role of the p33:p33/p92 interaction domain in RNA replication and intracellular localization of p33 and p92 proteins of Cucumber necrosis tombusvirus
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 338:81-95. 2005
    ....
  41. ncbi request reprint The overlapping RNA-binding domains of p33 and p92 replicase proteins are essential for tombusvirus replication
    Zivile Panaviene
    Department of Plant Pathology, Agricultural Science Building N, University of Kentucky, Lexington, KY 40546, USA
    Virology 308:191-205. 2003
    ..Interestingly, mutations within the RPR motif of p33 and p92 had different effects on RNA replication, suggesting different roles for the RNA-binding motifs of these proteins in tombusvirus replication...
  42. pmc Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Proc Natl Acad Sci U S A 102:7326-31. 2005
    ..This finding suggests that virus infection and disease-causing proteins might use or alter similar host pathways and may suggest connections between chronic diseases and prior virus infection...
  43. pmc The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication
    Sandra Monkewich
    Department of Biology, York University, 4700 Keele St, Toronto, Ontario, Canada M3J 1P3
    J Virol 79:4848-58. 2005
    ..Together, these results suggest that IRE activity is required in the cytosol at an early step in the viral replication process, such as template recruitment and/or replicase complex assembly...
  44. pmc Characterization of the RNA-binding domains in the replicase proteins of tomato bushy stunt virus
    K S Rajendran
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 77:9244-58. 2003
    ..Interestingly, the location of one of the RNA-binding domains in p92 is similar to the RNA-binding domain of the NS5B RdRp protein of hepatitis C virus...
  45. pmc Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity
    Zivile Panaviene
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546 0312, USA
    J Virol 78:8254-63. 2004
    ..In summary, this paper reports the first purified recombinant tombusvirus replicase showing high activity and template dependence, a finding that will greatly facilitate future studies on RNA replication in vitro...
  46. ncbi request reprint Advances in the molecular biology of tombusviruses: gene expression, genome replication, and recombination
    K Andrew White
    Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3
    Prog Nucleic Acid Res Mol Biol 78:187-226. 2004
    ..In this review, we compile recent information on each of the aforementioned processes-translation, transcription, replication and recombination-and discuss the significance of the results...
  47. ncbi request reprint Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance
    KaJohn Boonrod
    Centrum Grüne Gentechnik, Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Breitenweg 71, D 67435 Neustadt, Germany
    Nat Biotechnol 22:856-62. 2004
    ..Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses...
  48. ncbi request reprint Mutations in the RNA-binding domains of tombusvirus replicase proteins affect RNA recombination in vivo
    Zivile Panaviene
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 317:359-72. 2003
    ..Together with data in the article cited above, results presented here firmly establish that the conserved RNA-binding motif of the replicase proteins is involved in RNA replication, subgenomic RNA synthesis, and RNA recombination...
  49. pmc Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro
    Chi Ping Cheng
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 77:12033-47. 2003
    ..Demonstration of the ability of carmo- and tombusvirus RdRps to switch RNA templates in vitro supports the copy-choice models of RNA recombination and DI RNA formation for these viruses...
  50. pmc A replication silencer element in a plus-strand RNA virus
    Judit Pogany
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    EMBO J 22:5602-11. 2003
    ..Because similar silencer-promoter interactions are also predicted in other virus genera, this type of RNA-based regulatory mechanism may represent a widely utilized strategy for modulating replication...
  51. ncbi request reprint Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 314:315-25. 2003
    ..In summary, development of yeast as a host for replication of TBSV DI RNA will facilitate studies on the roles of viral and host proteins in replication/recombination...
  52. doi request reprint Multiple roles of viral replication proteins in plant RNA virus replication
    Peter D Nagy
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Methods Mol Biol 451:55-68. 2008
    ....
  53. pmc Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination
    Elena Serviene
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    J Virol 80:1231-41. 2006
    ..Overall, this and previous works firmly establish that a set of essential and nonessential host genes could affect TBSV recombination and evolution...
  54. pmc Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication
    Saulius Serva
    Department of Plant Pathology, University of Kentucky, Lexington, 40546 0312, USA
    J Virol 80:2162-9. 2006
    ....
  55. pmc Suppression of viral RNA recombination by a host exoribonuclease
    Chi Ping Cheng
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, Kentucky 40546, USA
    J Virol 80:2631-40. 2006
    ..Altogether, this paper establishes that host ribonucleases and host-mediated viral RNA turnover play major roles in RNA virus recombination and evolution...
  56. pmc Identification of essential host factors affecting tombusvirus RNA replication based on the yeast Tet promoters Hughes Collection
    Yi Jiang
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    J Virol 80:7394-404. 2006
    ..Sci. USA 102:7326-7331, 2005) led to the identification of 126 host genes (out of approximately 5,600 genes that represent approximately 95% of all the known and predicted yeast genes) that affected the accumulation of tombusvirus RNA...
  57. ncbi request reprint Exploiting alternative subcellular location for replication: tombusvirus replication switches to the endoplasmic reticulum in the absence of peroxisomes
    Magdalena Jonczyk
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546 0312, USA
    Virology 362:320-30. 2007
    ..This suggests that RNA viruses might have remarkable flexibility for using various host membranes for their replication...
  58. ncbi request reprint Expression of the Arabidopsis Xrn4p 5'-3' exoribonuclease facilitates degradation of tombusvirus RNA and promotes rapid emergence of viral variants in plants
    Chi Ping Cheng
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    Virology 368:238-48. 2007
    ..benthamiana protoplasts, whereas one CNV variant caused novel symptoms and moved systemically in N. benthamiana plants. Altogether, this paper establishes that a single plant gene can contribute to the emergence of novel viral variants...
  59. ncbi request reprint Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins
    Hannah M Jaag
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    Virology 368:388-404. 2007
    ..We propose a model that the concentration (molecular crowding) of replication proteins within the viral replicase is a factor affecting viral replication and recombination...
  60. doi request reprint Tomato bushy stunt virus co-opts the RNA-binding function of a host metabolic enzyme for viral genomic RNA synthesis
    Robert Yung Liang Wang
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Cell Host Microbe 3:178-87. 2008
    ..Downregulation of GAPDH in a natural plant host decreased TBSV genomic RNA accumulation. Thus, TBSV co-opts the RNA-binding function of a metabolic protein, helping convert the host cell into a viral factory...
  61. ncbi request reprint Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly
    K S Rajendran
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 345:270-9. 2006
    ....
  62. doi request reprint Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus
    K S Rajendran
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Methods Mol Biol 451:267-77. 2008
    ..SPR-based biosensor BIAcore X was used to carry out TBSV replicase protein interaction studies...
  63. doi request reprint Genome-wide screens for identification of host factors in viral replication
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Methods Mol Biol 451:615-24. 2008
    ..Here, we describe procedures, which facilitate high-throughput analysis of tombusvirus replication in yeast...
  64. pmc Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast
    Judit Pogany
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    J Virol 82:5967-80. 2008
    ....
  65. ncbi request reprint Inhibition of in vitro RNA binding and replicase activity by phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus
    Jozsef Stork
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 343:79-92. 2005
    ..Thus, phosphorylation of p33 might help in switching the role of the viral RNA from replication to other processes, such as viral RNA encapsidation and cell-to-cell movement in infected hosts...
  66. pmc Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein
    Zhenghe Li
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    J Virol 82:6911-26. 2008
    ..In addition, we have shown that p33 becomes ubiquitinated in vivo. We propose that ubiquitination of p33 likely alters its function or affects the recruitment of host factors during TBSV replication...
  67. doi request reprint The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins
    Kunj B Pathak
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 379:294-305. 2008
    ..These data support the model that Pex19p is a cellular transporter for localization of p33 replication protein to the host peroxisomal membranes...
  68. ncbi request reprint Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication
    Natalia Shapka
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 343:65-78. 2005
    ....
  69. pmc Genome-wide screen identifies host genes affecting viral RNA recombination
    Elena Serviene
    Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY 40546, USA
    Proc Natl Acad Sci U S A 102:10545-50. 2005
    ..Overall, our results demonstrate that a set of host genes have a major effect on RNA virus recombination and evolution...

Research Grants4

  1. Dissecting the mechanism of viral RNA recombination
    Peter Nagy; Fiscal Year: 2004
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and safer vaccines. ..
  2. Host factors involved in viral RNA recombination
    Peter Nagy; Fiscal Year: 2006
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and to develop safer vaccines. ..
  3. Role of an essential RNA chaperone in virus replication
    Peter Nagy; Fiscal Year: 2006
    ....
  4. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2008
    ..The tractable in vitro and in vivo TBSV system developed by the investigator could prove beneficial to studies of other, less amenable RNA viruses. [unreadable] [unreadable] [unreadable]..