Shaohu Sheng

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint External nickel inhibits epithelial sodium channel by binding to histidine residues within the extracellular domains of alpha and gamma subunits and reducing channel open probability
    Shaohu Sheng
    Renal Electrolyte Division, The Department of Medicine, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 277:50098-111. 2002
  2. ncbi request reprint Epithelial Na+ channels are fully activated by furin- and prostasin-dependent release of an inhibitory peptide from the gamma-subunit
    James B Bruns
    Renal Electrolyte Division, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:6153-60. 2007
  3. pmc Extracellular allosteric regulatory subdomain within the gamma subunit of the epithelial Na+ channel
    Katie L Winarski
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:26088-96. 2010
  4. ncbi request reprint Functional role of extracellular loop cysteine residues of the epithelial Na+ channel in Na+ self-inhibition
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 282:20180-90. 2007
  5. ncbi request reprint Extracellular histidine residues crucial for Na+ self-inhibition of epithelial Na+ channels
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:9743-9. 2004
  6. ncbi request reprint Extracellular Zn2+ activates epithelial Na+ channels by eliminating Na+ self-inhibition
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:31687-96. 2004
  7. ncbi request reprint Side chain orientation of residues lining the selectivity filter of epithelial Na+ channels
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:8513-22. 2005
  8. ncbi request reprint On the interaction between amiloride and its putative alpha-subunit epithelial Na+ channel binding site
    Ossama B Kashlan
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:26206-15. 2005
  9. ncbi request reprint Mutations in the pore region modify epithelial sodium channel gating by shear stress
    Marcelo D Carattino
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, PA 15231, USA
    J Biol Chem 280:4393-401. 2005
  10. pmc Novel determinants of epithelial sodium channel gating within extracellular thumb domains
    Ahmad B Maarouf
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, PA 15261, USA
    J Biol Chem 284:7756-65. 2009

Collaborators

Detail Information

Publications24

  1. ncbi request reprint External nickel inhibits epithelial sodium channel by binding to histidine residues within the extracellular domains of alpha and gamma subunits and reducing channel open probability
    Shaohu Sheng
    Renal Electrolyte Division, The Department of Medicine, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 277:50098-111. 2002
    ..Our results suggest that external Ni(2+) reduces ENaC open probability by binding to a site consisting of alphaHis(282) and gammaHis(239) and that these histidine residues may participate in ENaC gating...
  2. ncbi request reprint Epithelial Na+ channels are fully activated by furin- and prostasin-dependent release of an inhibitory peptide from the gamma-subunit
    James B Bruns
    Renal Electrolyte Division, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:6153-60. 2007
    ..Our results suggest that multiple proteases cleave ENaC gamma subunits to fully activate the channel...
  3. pmc Extracellular allosteric regulatory subdomain within the gamma subunit of the epithelial Na+ channel
    Katie L Winarski
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:26088-96. 2010
    ..We conclude that gammaHis(239) functions within an extracellular allosteric regulatory subdomain of the gamma subunit that has an important role in conferring the response of the channel to external Na(+)...
  4. ncbi request reprint Functional role of extracellular loop cysteine residues of the epithelial Na+ channel in Na+ self-inhibition
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 282:20180-90. 2007
    ....
  5. ncbi request reprint Extracellular histidine residues crucial for Na+ self-inhibition of epithelial Na+ channels
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:9743-9. 2004
    ..These results suggest that these two histidine residues within the extracellular loops are crucial structural determinants for Na(+) self-inhibition...
  6. ncbi request reprint Extracellular Zn2+ activates epithelial Na+ channels by eliminating Na+ self-inhibition
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:31687-96. 2004
    ..Our results suggest that external Zn(2+) activates ENaC by relieving the channel from Na(+) self-inhibition, and that external Ni(2+) mimics or masks Na(+) self-inhibition...
  7. ncbi request reprint Side chain orientation of residues lining the selectivity filter of epithelial Na+ channels
    Shaohu Sheng
    Renal Electrolyte Division, Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:8513-22. 2005
    ....
  8. ncbi request reprint On the interaction between amiloride and its putative alpha-subunit epithelial Na+ channel binding site
    Ossama B Kashlan
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:26206-15. 2005
    ....
  9. ncbi request reprint Mutations in the pore region modify epithelial sodium channel gating by shear stress
    Marcelo D Carattino
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, PA 15231, USA
    J Biol Chem 280:4393-401. 2005
    ....
  10. pmc Novel determinants of epithelial sodium channel gating within extracellular thumb domains
    Ahmad B Maarouf
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, PA 15261, USA
    J Biol Chem 284:7756-65. 2009
    ..Our results demonstrate that alphaGly(481) and gammaMet(438) are functional determinants of Na(+) self-inhibition and of ENaC gating and suggest that the thumb domain contributes to the channel gating machinery...
  11. ncbi request reprint The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit
    Marcelo D Carattino
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:18901-7. 2006
    ..Our results suggest that proteolysis of the alpha subunit activates ENaC by disassociating an inhibitory domain (alphaAsp-206-Arg-231) from its effector site within the channel complex...
  12. ncbi request reprint Furin cleavage activates the epithelial Na+ channel by relieving Na+ self-inhibition
    Shaohu Sheng
    Dept of Medicine, Univ of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Am J Physiol Renal Physiol 290:F1488-96. 2006
    ..Moreover, we demonstrate for the first time a clear relationship between ENaC P(o) and extracellular [Na+], supporting the notion that Na+ self-inhibition reflects a P(o) reduction due to high extracellular [Na+]...
  13. ncbi request reprint Multiple epithelial Na+ channel domains participate in subunit assembly
    James B Bruns
    Department of Medicine, University of Pittsburgh, 15261, USA
    Am J Physiol Renal Physiol 285:F600-9. 2003
    ..These data suggest that multiple sites within the alpha-subunit participate in subunit-subunit interactions that are required for proper assembly of the heterooligomeric ENaC complex...
  14. pmc Base of the thumb domain modulates epithelial sodium channel gating
    Shujie Shi
    Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 286:14753-61. 2011
    ....
  15. pmc Gain-of-function variant of the human epithelial sodium channel
    Jingxin Chen
    Dept of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Am J Physiol Renal Physiol 304:F207-13. 2013
    ..We conclude that γL511Q is a gain-of-function human ENaC variant. Our results suggest that γL511Q enhances ENaC activity by increasing channel open probability and dampens channel regulation by extracellular Na(+) and proteases...
  16. ncbi request reprint Epithelial Na+ channels are activated by laminar shear stress
    Marcelo D Carattino
    Renal Electrolyte Division, Departments of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:4120-6. 2004
    ..Our results suggest that shear stress activates ENaC by modifying the gating properties of the channel...
  17. ncbi request reprint Functional polymorphism in the carboxyl terminus of the alpha-subunit of the human epithelial sodium channel
    Frederick F Samaha
    Department of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    J Biol Chem 279:23900-7. 2004
    ..Our results suggest that halphaT663A is a functional polymorphism that affects human ENaC surface expression...
  18. pmc Deletion of α-subunit exon 11 of the epithelial Na+ channel reveals a regulatory module
    Jingxin Chen
    Renal Electrolyte Div, Univ of Pittsburgh, 3550 Terrace St, Pittsburgh, PA 15261
    Am J Physiol Renal Physiol 306:F561-7. 2014
    ..Our results suggest that exon 11 within the three human ENaC genes encodes structurally homologous yet functionally diverse domains and that exon 11 in the α-subunit encodes a module that regulates channel gating. ..
  19. pmc Probing the structural basis of Zn2+ regulation of the epithelial Na+ channel
    Jingxin Chen
    Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 287:35589-98. 2012
    ....
  20. pmc External Cu2+ inhibits human epithelial Na+ channels by binding at a subunit interface of extracellular domains
    Jingxin Chen
    Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 286:27436-46. 2011
    ..We conclude that extracellular Cu(2+) is a potent inhibitor of human ENaC and binds to multiple sites within the extracellular domains including a subunit interface...
  21. pmc Human epithelial Na+ channel missense variants identified in the GenSalt study alter channel activity
    Evan C Ray
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania
    Am J Physiol Renal Physiol 311:F908-F914. 2016
    ..This study identifies novel functional human ENaC variants and demonstrates that some variants alter channel cell surface expression and/or Na+ self-inhibition...
  22. pmc Blood pressure and amiloride-sensitive sodium channels in vascular and renal cells
    David G Warnock
    Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 34294 0007, USA
    Nat Rev Nephrol 10:146-57. 2014
    ....
  23. ncbi request reprint Asymmetric organization of the pore region of the epithelial sodium channel
    Jinqing Li
    Department of Physiology, University of Pennsylvania, Philadelphia 19104, USA
    J Biol Chem 278:13867-74. 2003
    ..Our results, placed in context with previous observations, suggest that pore regions from the three ENaC subunits have an asymmetric organization...
  24. ncbi request reprint Biophysical approach to determine the subunit stoichiometry of the epithelial sodium channel using the Xenopus laevis oocyte expression system
    Farhad Kosari
    Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA
    Methods Mol Biol 337:53-63. 2006
    ..Details related to the expression of wild type and mutant ENaCs in Xenopus oocytes and the examination of blocker sensitivity by two-electrode voltage clamp, as well as analysis of data are provided...