Guido Zampighi

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc The three-dimensional distribution of αA-crystalline in rat lenses and its possible relation to transparency
    Guido A Zampighi
    Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America
    PLoS ONE 6:e23753. 2011
  2. pmc Conical electron tomography of a chemical synapse: polyhedral cages dock vesicles to the active zone
    Guido A Zampighi
    Department of Neurobiology, Jules Stein Eye Research Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90095, USA
    J Neurosci 28:4151-60. 2008
  3. pmc Conical electron tomography of a chemical synapse: vesicles docked to the active zone are hemi-fused
    G A Zampighi
    Departments of Neurobiology, Physiology, and Jules Stein Eye Research Institute, University of California at Los Angeles School of Medicine, Los Angeles, California, USA
    Biophys J 91:2910-8. 2006
  4. ncbi request reprint Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels
    Guido A Zampighi
    Department of Neurobiology and Jules Stein Eye Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
    Invest Ophthalmol Vis Sci 46:3247-55. 2005
  5. ncbi request reprint Conical tomography II: A method for the study of cellular organelles in thin sections
    G A Zampighi
    Department of Neurobiology, David Geffen School of Medicine, University California Los Angeles, USA
    J Struct Biol 151:263-74. 2005
  6. ncbi request reprint Structure of functional single AQP0 channels in phospholipid membranes
    Guido A Zampighi
    Department of Neurobiology, UCLA School of Medicine, Los Angeles, CA 90095 1763, USA
    J Mol Biol 325:201-10. 2003
  7. ncbi request reprint Micro-domains of AQP0 in lens equatorial fibers
    Guido A Zampighi
    Department of Neurobiology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Exp Eye Res 75:505-19. 2002
  8. ncbi request reprint Epithelial organization of the mammalian lens
    G A Zampighi
    Departments of Neurobiology and Physiology, School of Medicine, Los Angeles, CA 90095, USA
    Exp Eye Res 71:415-35. 2000
  9. pmc Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes
    G A Zampighi
    Department of Neurobiology, University of California, Los Angeles, School of Medicine, Los Angeles, California 90095, USA
    J Gen Physiol 113:507-24. 1999
  10. ncbi request reprint Inhibition of gap junction hemichannels by chloride channel blockers
    S Eskandari
    Department of Physiology, School of Medicine, University of California, LosAngeles 90095 1751, USA
    J Membr Biol 185:93-102. 2002

Research Grants

  1. TECNIA 12 BioTWIN Transmission Electron Microscope
    Guido Zampighi; Fiscal Year: 2003
  2. A Method To Study Membrane Protein Structure
    Guido Zampighi; Fiscal Year: 2003

Detail Information

Publications23

  1. pmc The three-dimensional distribution of αA-crystalline in rat lenses and its possible relation to transparency
    Guido A Zampighi
    Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America
    PLoS ONE 6:e23753. 2011
    ..It thus seems likely that lost or gain of long-range order determines the 3D-structure of the fiber cell and possible also cataract formation...
  2. pmc Conical electron tomography of a chemical synapse: polyhedral cages dock vesicles to the active zone
    Guido A Zampighi
    Department of Neurobiology, Jules Stein Eye Research Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90095, USA
    J Neurosci 28:4151-60. 2008
    ....
  3. pmc Conical electron tomography of a chemical synapse: vesicles docked to the active zone are hemi-fused
    G A Zampighi
    Departments of Neurobiology, Physiology, and Jules Stein Eye Research Institute, University of California at Los Angeles School of Medicine, Los Angeles, California, USA
    Biophys J 91:2910-8. 2006
    ..In conclusion, our observations in cortical synapses strengthen the hypothesis that hemi-fusion is a stable intermediary that precedes full fusion and release...
  4. ncbi request reprint Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels
    Guido A Zampighi
    Department of Neurobiology and Jules Stein Eye Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
    Invest Ophthalmol Vis Sci 46:3247-55. 2005
    ..The current study was conducted to test the hypothesis that regulation of lens coupling by activation of protein kinase Cgamma (PKCgamma) affects the number of channels composed of Cx46, Cx50, or both connexins...
  5. ncbi request reprint Conical tomography II: A method for the study of cellular organelles in thin sections
    G A Zampighi
    Department of Neurobiology, David Geffen School of Medicine, University California Los Angeles, USA
    J Struct Biol 151:263-74. 2005
    ..We concluded that conical electron tomography of thin-sectioned specimens provides a powerful experimental approach for studying thin-sectioned neuronal organelles at resolution levels of approximately 4 nm...
  6. ncbi request reprint Structure of functional single AQP0 channels in phospholipid membranes
    Guido A Zampighi
    Department of Neurobiology, UCLA School of Medicine, Los Angeles, CA 90095 1763, USA
    J Mol Biol 325:201-10. 2003
    ..We suggest that the discrepancy might reflect differences in the location of the amino and carboxyl termini in the crystal and in the phospholipid bilayer...
  7. ncbi request reprint Micro-domains of AQP0 in lens equatorial fibers
    Guido A Zampighi
    Department of Neurobiology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Exp Eye Res 75:505-19. 2002
    ..We concluded that the ability of AQP0 to arrange itself in micro-domains conferred functional properties that might contribute to the maintenance of lens transparency and homeostasis...
  8. ncbi request reprint Epithelial organization of the mammalian lens
    G A Zampighi
    Departments of Neurobiology and Physiology, School of Medicine, Los Angeles, CA 90095, USA
    Exp Eye Res 71:415-35. 2000
    ....
  9. pmc Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes
    G A Zampighi
    Department of Neurobiology, University of California, Los Angeles, School of Medicine, Los Angeles, California 90095, USA
    J Gen Physiol 113:507-24. 1999
    ..These exocytotic and endocytotic rates suggest that the entire plasma membrane of the oocyte is replaced in approximately 24 h...
  10. ncbi request reprint Inhibition of gap junction hemichannels by chloride channel blockers
    S Eskandari
    Department of Physiology, School of Medicine, University of California, LosAngeles 90095 1751, USA
    J Membr Biol 185:93-102. 2002
    ....
  11. ncbi request reprint Five transmembrane helices form the sugar pathway through the Na+/glucose cotransporter
    M Panayotova-Heiermann
    Department of Physiology, UCLA Medical Center, Los Angeles, California 90095 1751, USA
    J Biol Chem 272:20324-7. 1997
    ....
  12. pmc Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy
    S Eskandari
    Department of Physiology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1751, USA
    Proc Natl Acad Sci U S A 95:11235-40. 1998
    ....
  13. ncbi request reprint Regulation of Na+/glucose cotransporters
    E M Wright
    Department of Physiology, UCLA School of Medicine 90095 1751, USA
    J Exp Biol 200:287-93. 1997
    ....
  14. ncbi request reprint Number of subunits comprising the epithelial sodium channel
    S Eskandari
    Department of Physiology, UCLA School of Medicine, Los Angeles, California 90095 1751, USA
    J Biol Chem 274:27281-6. 1999
    ..These data suggest that functional ENaC consists of eight or nine subunits of which a minimum of two are gamma subunits...
  15. pmc Pentameric assembly of a neuronal glutamate transporter
    S Eskandari
    Departments of Physiology and Neurobiology, University of California at Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles, CA 90095 1751, USA
    Proc Natl Acad Sci U S A 97:8641-6. 2000
    ..The pentameric structure of EAAT3 offers new insights into its function as both a glutamate transporter and a glutamate-gated chloride channel...
  16. ncbi request reprint Polyhedral protein cages encase synaptic vesicles and participate in their attachment to the active zone
    G A Zampighi
    Department of Neurobiology, UCLA School of Medicine 90095 1763, USA
    J Struct Biol 119:347-59. 1997
    ..Therefore, current models of transmitter release should take into account the substantial heterogeneity of the vesicle population and the binding of vesicular cages to the membrane of the active zone...
  17. ncbi request reprint A novel calcium-independent peripheral membrane-bound form of annexin B12
    Balachandra G Hegde
    Department of Biochemistry and Molecular Biology, Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    Biochemistry 45:934-42. 2006
    ..0 but none at pH 5.3. Together, these data raise the possibility that the peripheral-bound form of annexin B12 could act as a kinetic intermediate in the formation of the transmembrane form of the protein...
  18. pmc The structure of the cytoplasm of lens fibers as determined by conical tomography
    C Schietroma
    Department of Neurobiology, UCLA School of Medicine, Los Angeles, CA, USA
    Exp Eye Res 88:566-74. 2009
    ..Loss of either function likely would play a significant role in cataract formation...
  19. ncbi request reprint Protein kinase C-gamma activation in the early streptozotocin diabetic rat lens
    Dingbo Lin
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    Curr Eye Res 32:523-32. 2007
    ..The purpose of this study is to demonstrate the early activation of the protein kinase C-gamma (PKC-gamma) pathway in the streptozotocin (STZ)-induced diabetic rat lens...
  20. ncbi request reprint PKCgamma knockout mouse lenses are more susceptible to oxidative stress damage
    Dingbo Lin
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    J Exp Biol 209:4371-8. 2006
    ..The absence of this regulatory mechanism in the PKCgamma knockout mice may cause those lenses to have increased susceptibility to oxidative damage...
  21. pmc Nerve growth factor signals via preexisting TrkA receptor oligomers
    Paul S Mischel
    Department of Pathology and Laboratory Medicine, UCLA School of Medicine, University of California, Los Angeles, California 90095 1732 USA
    Biophys J 83:968-76. 2002
    ..These results indicate that in Xenopus oocytes, NGF activates signaling via pre-formed TrkA receptor oligomers...
  22. ncbi request reprint Coupled sodium/glucose cotransport by SGLT1 requires a negative charge at position 454
    Ana Diez-Sampedro
    Department of Physiology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Los Angeles, California 90095 1751, USA
    Biochemistry 43:13175-84. 2004
    ..A positive charge on residue 454, in contrast, uncoupled Na(+) and sugar transport, indicating the importance of the negative charge in the coordination of the cotransport mechanism...
  23. ncbi request reprint Distribution of connexin50 channels and hemichannels in lens fibers: a structural approach
    Guido A Zampighi
    Department of Neurobiology, David Geffen School of Medicine, Los Angeles, CA, USA
    Cell Commun Adhes 10:265-70. 2003
    ..Therefore, in lens fibers, Cx50 hemichannels are inserted via exocytosis and are rapidly assembled into channels assembled in gap junction plaques...

Research Grants2

  1. TECNIA 12 BioTWIN Transmission Electron Microscope
    Guido Zampighi; Fiscal Year: 2003
    ..abstract_text> ..
  2. A Method To Study Membrane Protein Structure
    Guido Zampighi; Fiscal Year: 2003
    ....