James Crowe

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. doi request reprint Teaching a Clone to Walk, One Step at a Time
    James E Crowe
    Departments of Pediatrics, Pathology, Microbiology, and Immunology and Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA Electronic address
    Cell 166:1360-1. 2016
  2. doi request reprint Intracellular neutralization of a virus using a cell-penetrating molecular transporter
    Gopal Sapparapu
    The Vanderbilt Vaccine Center, Nashville, TN 37232, USA
    Nanomedicine (Lond) 9:1613-24. 2014
  3. pmc Influenza human monoclonal antibody 1F1 interacts with three major antigenic sites and residues mediating human receptor specificity in H1N1 viruses
    Tshidi Tsibane
    Department of Microbiology, Mount Sinai School of Medicine, New York City, New York, United States of America
    PLoS Pathog 8:e1003067. 2012
  4. pmc An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody
    Jens C Krause
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    MBio 2:e00345-10. 2011
  5. pmc Human peripheral blood antibodies with long HCDR3s are established primarily at original recombination using a limited subset of germline genes
    Bryan S Briney
    Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 7:e36750. 2012
  6. pmc Location and length distribution of somatic hypermutation-associated DNA insertions and deletions reveals regions of antibody structural plasticity
    B S Briney
    Departments of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232 0417, USA
    Genes Immun 13:523-9. 2012
  7. pmc Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins
    Fyza Y Shaikh
    Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 7:e40826. 2012
  8. pmc Respiratory syncytial virus regulates human microRNAs by using mechanisms involving beta interferon and NF-κB
    Natalie J Thornburg
    The Vanderbilt Vaccine Center, Nashville, Tennessee, USA
    MBio 3:. 2012
  9. pmc Human germline antibody gene segments encode polyspecific antibodies
    Jordan R Willis
    Center for Structural Biology, Vanderbilt University, Nashville, Tennessee, USA
    PLoS Comput Biol 9:e1003045. 2013
  10. pmc Human rotavirus VP6-specific antibodies mediate intracellular neutralization by binding to a quaternary structure in the transcriptional pore
    Mohammed S Aiyegbo
    Department of Pathology, Microbiology and Immunology, Vanderbilt Medical Center, Nashville, Tennessee, USA
    PLoS ONE 8:e61101. 2013

Research Grants

Collaborators

Detail Information

Publications75

  1. doi request reprint Teaching a Clone to Walk, One Step at a Time
    James E Crowe
    Departments of Pediatrics, Pathology, Microbiology, and Immunology and Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA Electronic address
    Cell 166:1360-1. 2016
    ....
  2. doi request reprint Intracellular neutralization of a virus using a cell-penetrating molecular transporter
    Gopal Sapparapu
    The Vanderbilt Vaccine Center, Nashville, TN 37232, USA
    Nanomedicine (Lond) 9:1613-24. 2014
    ..Once viruses enter cells, conventional antibodies cannot inhibit virus infection or replication. It is desirable to develop an efficient and nontoxic method for the introduction of virus-inhibiting antibodies into cells...
  3. pmc Influenza human monoclonal antibody 1F1 interacts with three major antigenic sites and residues mediating human receptor specificity in H1N1 viruses
    Tshidi Tsibane
    Department of Microbiology, Mount Sinai School of Medicine, New York City, New York, United States of America
    PLoS Pathog 8:e1003067. 2012
    ..Therefore, 1F1 heavy-chain interactions with conserved RBS residues likely contribute to its ability to inhibit divergent HAs...
  4. pmc An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody
    Jens C Krause
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    MBio 2:e00345-10. 2011
    ..Removal of the insertion restores the canonical structure of CDR H1 and CDR H2, but binding, neutralization activity, and in vivo activity were reduced markedly because of steric conflict of CDR H1 with the hemagglutinin antigen...
  5. pmc Human peripheral blood antibodies with long HCDR3s are established primarily at original recombination using a limited subset of germline genes
    Bryan S Briney
    Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 7:e36750. 2012
    ....
  6. pmc Location and length distribution of somatic hypermutation-associated DNA insertions and deletions reveals regions of antibody structural plasticity
    B S Briney
    Departments of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232 0417, USA
    Genes Immun 13:523-9. 2012
    ..Finally, through the analysis of both location and length distribution of SHA indels, we have determined regions of structural plasticity within the antibody protein...
  7. pmc Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins
    Fyza Y Shaikh
    Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 7:e40826. 2012
    ....
  8. pmc Respiratory syncytial virus regulates human microRNAs by using mechanisms involving beta interferon and NF-κB
    Natalie J Thornburg
    The Vanderbilt Vaccine Center, Nashville, Tennessee, USA
    MBio 3:. 2012
    ..RSV appears to manipulate host cell gene expression through regulation of expression of miRNAs related to the interferon response. The data suggest a new mechanism of virus-host cell interactions for paramyxoviruses...
  9. pmc Human germline antibody gene segments encode polyspecific antibodies
    Jordan R Willis
    Center for Structural Biology, Vanderbilt University, Nashville, Tennessee, USA
    PLoS Comput Biol 9:e1003045. 2013
    ..Computational design of antibodies capable of binding multiple antigens may allow the rational design of antibodies that retain polyspecificity for diverse epitope binding...
  10. pmc Human rotavirus VP6-specific antibodies mediate intracellular neutralization by binding to a quaternary structure in the transcriptional pore
    Mohammed S Aiyegbo
    Department of Pathology, Microbiology and Immunology, Vanderbilt Medical Center, Nashville, Tennessee, USA
    PLoS ONE 8:e61101. 2013
    ..This unique mucosal mechanism of viral neutralization, which is not apparent from conventional immunoassays, may contribute significantly to human immunity to RV...
  11. pmc The potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1C19 reveals a unique cross-reactive epitope on the bc loop of domain II of the envelope protein
    Scott A Smith
    Department of Medicine, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee, USA
    MBio 4:e00873-13. 2013
    ..Furthermore, the unique epitope revealed by 1C19 suggests a focus for rational vaccine design based on novel immunogens presenting cross-reactive neutralizing determinants...
  12. pmc A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface
    Guntur Fibriansah
    Program in Emerging Infectious Diseases, Duke NUS Graduate Medical School, Singapore City, Singapore
    EMBO Mol Med 6:358-71. 2014
    ..We also showed that HMAb 1F4 can neutralize DENV at different stages of viral entry in a cell type and receptor dependent manner. The structure reveals the mechanism by which this potent and specific antibody blocks viral infection. ..
  13. doi request reprint Universal flu vaccines: primum non nocere
    James E Crowe
    Vanderbilt Vaccine Center, Vanderbilt University, Nashville, TN 37232, USA
    Sci Transl Med 5:200fs34. 2013
    ..this issue). ..
  14. pmc Influenza virus resistance to human neutralizing antibodies
    James E Crowe
    Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA
    MBio 3:e00213-12. 2012
    ..The recent paper by O'Donnell et al. [C. D. O'Donnell et al., mBio 3(3):e00120-12, 2012] identifies a mechanism for antibody resistance that does not require escape from binding but rather achieves a greater efficiency in replication...
  15. pmc Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection
    Scott A Smith
    Department of Medicine, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee, USA
    J Virol 86:2665-75. 2012
    ..Such principles could be used in the rational design of vaccines that enhance the induction of neutralizing antibodies, while lowering the risk of dengue shock syndrome...
  16. pmc Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years
    Chin Fen Yang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA
    Virol J 6:138. 2009
    ..Human metapneumovirus (HMPV) is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion) gene sequences collected over a 20-year period...
  17. ncbi request reprint Respiratory syncytial virus vaccine development
    J E Crowe
    Department of Pediatrics, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232 2581, USA
    Vaccine 20:S32-7. 2001
    ..Numerous vaccine candidates are currently in early clinical trials. This paper reviews the significant obstacles to development of RSV vaccines, and the progress made to date...
  18. pmc Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches
    James E Crowe
    Vanderbilt Vaccine Center, Vanderbilt University School of Medicine, Nashville, TN 37232 2905, USA
    Vaccine 27:G47-51. 2009
    ..These studies also reveal evidence for lifelong persistence of immunity to some influenza viruses...
  19. ncbi request reprint Genetic and structural determinants of virus neutralizing antibodies
    J E Crowe
    Department of Pediatrics and Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    Immunol Res 23:135-45. 2001
    ....
  20. ncbi request reprint Influence of maternal antibodies on neonatal immunization against respiratory viruses
    J E Crowe
    Departments of Pediatrics and Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
    Clin Infect Dis 33:1720-7. 2001
    ..This article explores the current knowledge regarding the effect of maternal Abs on respiratory virus and measles virus immunization, and it reviews the current approaches to overcoming Ab-mediated immunosuppression...
  21. ncbi request reprint Immunology of viral respiratory tract infection in infancy
    James E Crowe
    Department of Pediatrics, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232 2581, USA
    Paediatr Respir Rev 4:112-9. 2003
    ....
  22. ncbi request reprint Human metapneumovirus as a major cause of human respiratory tract disease
    James E Crowe
    Departments of Pediatrics and Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
    Pediatr Infect Dis J 23:S215-21. 2004
    ..The virus, first isolated in 2001, is a clear cause of lower respiratory tract disease in both the very young and the frail elderly. The virus causes acute wheezing in children or, less commonly, croup or pneumonia...
  23. pmc Human metapneumovirus fusion protein vaccines that are immunogenic and protective in cotton rats
    Gabriella Cseke
    Department of Chemistry, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Ave South, Nashville, TN 37232 2581, USA
    J Virol 81:698-707. 2007
    ..We conclude that sequence-optimized hMPV F protein protects against hMPV infection when delivered as either a DNA or a protein vaccine in cotton rats...
  24. ncbi request reprint Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Immunol Methods 275:223-37. 2003
    ....
  25. pmc An alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats
    Hoyin Mok
    Departments of Pediatrics, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Virol 82:11410-8. 2008
    ..Aberrant cytokine gene expression was not detected in these animals. Together, these results represent an important first step toward the use of VRPs encoding hMPV F proteins as a prophylactic vaccine for hMPV...
  26. ncbi request reprint Immunodominance of the VH1-46 antibody gene segment in the primary repertoire of human rotavirus-specific B cells is reduced in the memory compartment through somatic mutation of nondominant clones
    Cuixia Tian
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2905, USA
    J Immunol 180:3279-88. 2008
    ..Increased diversity in the VH gene repertoire of the two memory B cell groups derived from broader usage of VH gene segments, increased junctional diversity that was introduced by differential TdT activities, and somatic hypermutation...
  27. pmc The pediatric burden of human coronaviruses evaluated for twenty years
    H Keipp Talbot
    Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
    Pediatr Infect Dis J 28:682-7. 2009
    ..The epidemiology of human coronaviruses (HCoVs) has not been established using reverse transcription polymerase chain reaction techniques in a specimen collection that spans decades...
  28. ncbi request reprint CD154 regulates primate humoral immunity to influenza
    James E Crowe
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA
    Am J Transplant 3:680-8. 2003
    ..Furthermore, the data suggest that viral Ag stimulation of primates in the absence of CD154 stimulation may have a tolerizing effect on that Ag...
  29. ncbi request reprint Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Immunol 171:4680-8. 2003
    ..These data suggest that residual fetal bias of the B cell repertoire is not a limiting determinant of the quality of Ab responses to viruses of infants beyond the neonatal period...
  30. pmc Population-based incidence of human metapneumovirus infection among hospitalized children
    John V Williams
    Department of Pediatrics, Vanderbilt University Schoolof Medicine, and Monroe Carell Jr Children s Hospital at Vanderbilt, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 201:1890-8. 2010
    ..Human metapneumovirus (HMPV) is a leading cause of acute respiratory illness (ARI) in children. Population-based incidence rates and comprehensive clinical characterizations of disease have not been established...
  31. ncbi request reprint Natural evolution of a human virus-specific antibody gene repertoire by somatic hypermutation requires both hotspot-directed and randomly-directed processes
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Hum Immunol 66:666-76. 2005
    ..These findings suggest that virus-specific antibodies require both hotspot and randomly-directed processes...
  32. pmc Coronavirus infection and hospitalizations for acute respiratory illness in young children
    H Keipp B Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Med Virol 81:853-6. 2009
    ..2 (95% CI 4.3, 17.6), 4.2 (95% CI 1.9, 6.9), and 0 (95% CI 0, 3.7) in children aged <6 months, 6-23 months, and 24-59 months, respectively. Coronaviruses were identified in a modest number of hospitalized children...
  33. pmc Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation
    Aaron Derdowski
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Gen Virol 89:2698-708. 2008
    ..This work showed that HMPV N and P proteins provide the minimal viral requirements for HMPV inclusion body formation, which may be a distinguishing characteristic of members of the subfamily Pneumovirinae...
  34. ncbi request reprint Functional maturation of the human antibody response to rotavirus
    Nicole L Kallewaard
    Departments of Microbiology and Immunology, Vanderbilt University, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol 180:3980-9. 2008
    ..These structure-function studies suggest a molecular basis for the poor quality of Abs made in infancy following virus infection or immunization...
  35. pmc Human metapneumovirus infection plays an etiologic role in acute asthma exacerbations requiring hospitalization in adults
    John V Williams
    Departments of Pediatric, Vanderbilt University Medical Center, Nashville, TN 37232 8300, USA
    J Infect Dis 192:1149-53. 2005
    ..9-312]; P=.03). None of the patients with hMPV infection at hospitalization tested positive at follow-up, strongly suggesting that hMPV plays a direct etiologic role in acute asthma exacerbations...
  36. ncbi request reprint Rotavirus-specific CD5+ B cells in young children exhibit a distinct antibody repertoire compared with CD5- B cells
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, and Monroe Carell Jr Children s Hospital at Vanderbilt, Division of Neonatology, Nashville, TN 37232 9544, USA
    Hum Immunol 67:33-42. 2006
    ....
  37. pmc The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Infect Dis 193:387-95. 2006
    ..The role that human metapneumovirus (hMPV) plays in the etiology of upper respiratory tract infections (URIs) in children over a period of many years has not been evaluated previously...
  38. pmc Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells
    Cuixia Tian
    Department of Pediatrics, Vanderbilt University Medical Center, 21st Avenue South, Nashville, TN 37232, USA
    Mol Immunol 44:2173-83. 2007
    ..These data suggest distinct functional mechanisms underlying selection of this unique subset of un-class-switched memory B cells...
  39. pmc The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Virol 79:10944-51. 2005
    ..This model will allow further in vivo studies of hMPV pathogenesis and evaluation of vaccine candidates...
  40. pmc A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 192:1061-5. 2005
    ..Nine patients with hMPV developed LRI; 3 of these patients died. hMPV is a common cause of respiratory infections in adults with hematologic malignancies, with associated morbidity and mortality...
  41. pmc A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo
    John V Williams
    Pediatric Infectious Diseases, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232
    J Virol 81:8315-24. 2007
    ..There was a dose-response relationship between the dose of DS7 and virus titer. Human Fab DS7 may have prophylactic or therapeutic potential against severe hMPV infection...
  42. pmc Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivors
    Xiaocong Yu
    Departments of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nature 455:532-6. 2008
    ....
  43. ncbi request reprint VH1-46 is the dominant immunoglobulin heavy chain gene segment in rotavirus-specific memory B cells expressing the intestinal homing receptor alpha4beta7
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol 174:3454-60. 2005
    ..Interestingly, RV-specific intestinal homing IgD-/alpha4beta7+ B cells using the VH1-46 gene segment were not mutated, in contrast to systemic RV-specific IgD- B cells...
  44. pmc Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children
    John V Williams
    Division of Infectious Diseases, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    N Engl J Med 350:443-50. 2004
    ..We sought to determine the role of human metapneumovirus in lower respiratory tract illness in previously healthy infants and children...
  45. pmc Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses
    Jens C Krause
    Departments of Pediatrics and of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Virol 84:3127-30. 2010
    ..These antibodies exhibited high potency against the 2009 virus in vitro, and one exerted a marked therapeutic effect in vivo...
  46. pmc An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies
    Xiaocong Yu
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol Methods 336:142-51. 2008
    ..In conclusion, we have developed an efficient and routine approach for the generation of human hybridomas secreting functional human virus-specific monoclonal antibodies...
  47. pmc Venezuelan equine encephalitis virus replicon particles encoding respiratory syncytial virus surface glycoproteins induce protective mucosal responses in mice and cotton rats
    Hoyin Mok
    Department of Pediatrics, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Virol 81:13710-22. 2007
    ..These results represent an important first step toward the use of VRPs encoding RSV proteins as a prophylactic vaccine for RSV...
  48. pmc Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV
    Mark D Hicar
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2905, USA
    J Acquir Immune Defic Syndr 54:223-35. 2010
    ..These data demonstrate that Gag-Env pseudovirions recapitulate CD4 and coreceptor binding pocket antigenic structures and can facilitate identification of B-cell clones that secrete neutralizing antibodies...
  49. pmc The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations
    John V Williams
    Department of Pediatrics, Nashville, TN 37232, United States
    Mol Immunol 47:407-14. 2009
    ..These biased features of the RSV-specific repertoire likely contribute to the poor functional Ab response in very young infants...
  50. pmc Identification of a novel human leucocyte antigen-A*01-restricted cytotoxic T-lymphocyte epitope in the respiratory syncytial virus fusion protein
    Michael T Rock
    Departments of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37205, USA
    Immunology 108:474-80. 2003
    ..An HLA-A*01-restricted CTL epitope detected in three healthy adult subjects is characterized. This is the first RSV-specific memory CTL response identified in the fusion protein of RSV...
  51. pmc Examination of a fusogenic hexameric core from human metapneumovirus and identification of a potent synthetic peptide inhibitor from the heptad repeat 1 region
    Scott A Miller
    Vanderbilt University, Department of Chemistry, Station B 351822, Nashville, TN 37235 1822, USA
    J Virol 81:141-9. 2007
    ..Our studies provide evidence that hMPV also uses a coiled-coil structure as a major player in the fusion process. Additionally, viral HR-1 peptide sequences may need further investigation as potent fusion inhibitors...
  52. ncbi request reprint Transcriptional control of activation-induced cytidine deaminase and error-prone DNA polymerases is functionally mature in the B cells of infants at birth
    Amber L Bowen
    Department of Microbiology and Immunology, Vanderbilt University Medical Center, 1161 21st Avenue South, Nashville, TN 37232 2905, USA
    Hum Immunol 67:43-6. 2006
    ..These data suggest that the ability to upregulate gene transcription of enzymes mediating SHM is not a limiting determinant of the functional quality of infant antibody responses...
  53. pmc Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells
    Cuixia Tian
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2905, USA
    Immunology 119:54-62. 2006
    ..These studies suggest a possible mechanism underlying the limited magnitude and durability of neonatal antibody responses...
  54. pmc Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication
    Michael E Lindquist
    Departments of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2905, USA
    J Virol 84:12274-84. 2010
    ..These results suggest that RSV induces a host stress granule response and preferentially replicates in host cells that have committed to a stress response...
  55. ncbi request reprint Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes
    Elizabeth L Bentzen
    Department of Chemistry, Vanderbilt University, Station B 351822, Nashville, Tennessee 37235 1822, USA
    Nano Lett 5:591-5. 2005
    ..The implications of these results are that QDs may provide a method for early, rapid detection of viral infection and open the door for future studies of the intricate spatial features cell trafficking of viral proteins...
  56. pmc Amphiregulin-deficient mice develop spasmolytic polypeptide expressing metaplasia and intestinal metaplasia
    Ki Taek Nam
    Nashville Department of Veterans Affairs Medical Center, Nashville, Tennessee 37232 0443, USA
    Gastroenterology 136:1288-96. 2009
    ..We previously determined that loss of amphiregulin (AR) promotes SPEM induced by acute oxyntic atrophy. We have now examined whether SPEM in the AR-/- mouse predisposes the stomach to gastric neoplasia...
  57. pmc A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction
    Martin L Moore
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Virol 83:4185-94. 2009
    ..We identified the F protein of RSV line 19 as a factor that plays a role in pulmonary mucin expression in the setting of RSV infection...
  58. pmc Tissue-specific regulation of CD8+ T-lymphocyte immunodominance in respiratory syncytial virus infection
    Sujin Lee
    Department of Pediatrics, Vanderbilt University Medical Center, T 2220 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232 2905, USA
    J Virol 81:2349-58. 2007
    ..These studies will facilitate investigation of the regulation of immunodominance of RSV-specific CTL epitopes...
  59. pmc A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin
    Jens C Krause
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Virol 85:10905-8. 2011
    ..This antibody exposes a new mechanism underlying broad immunity against H1N1 influenza viruses and identifies a conserved epitope that might be incorporated into engineered H1 virus vaccines...
  60. pmc Homing in on acute graft vs. host disease: tissue-specific T regulatory and Th17 cells
    Brian G Engelhardt
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
    Curr Top Microbiol Immunol 341:121-46. 2010
    ..tumor effect while minimizing the potentially fatal side effects of aGVHD. A better understanding of Tregs and their tissue specificity should lead to improvement in the success of HSCT...
  61. pmc The transmembrane domain of the respiratory syncytial virus F protein is an orientation-independent apical plasma membrane sorting sequence
    Sean C Brock
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Virol 79:12528-35. 2005
    ....
  62. pmc Effect of zinc salts on respiratory syncytial virus replication
    Rahaman O Suara
    Department of Pediatric, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Antimicrob Agents Chemother 48:783-90. 2004
    ..The results of this study suggest that zinc mediates antiviral activity on RSV by altering the ability of the cell to support RSV replication...
  63. ncbi request reprint Vaccination success rate and reaction profile with diluted and undiluted smallpox vaccine: a randomized controlled trial
    Thomas R Talbot
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA s
    JAMA 292:1205-12. 2004
    ....
  64. ncbi request reprint Smallpox vaccination does not elevate systemic levels of prothrombotic proteins associated with ischemic cardiac events
    Julia F Shaklee
    Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Infect Dis 191:724-30. 2005
    ....
  65. ncbi request reprint Differential regulation of granzyme and perforin in effector and memory T cells following smallpox immunization
    Michael T Rock
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol 174:3757-64. 2005
    ..Together, these data suggest an important role for effector CD8+ T cells in controlling poxvirus infection, and have implications for our understanding of human CD8+ T cell differentiation...
  66. ncbi request reprint Prevalence of reovirus-specific antibodies in young children in Nashville, Tennessee
    Jennifer H Tai
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 191:1221-4. 2005
    ..We observed an age-dependent increase in reovirus-specific antibodies in children 1 year of age and older, peaking at 50.0% in children 5-6 years of age. These findings suggest that reovirus infections are common during early childhood...
  67. pmc Emerging studies of human HIV-specific antibody repertoires
    Mark D Hicar
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Vaccine 28:B18-23. 2010
    ..The rapid isolation of large panels of novel human neutralizing antibodies promises to reveal new insights into the fundamental principles underlying antibody-mediated neutralization of HIV...
  68. pmc Prospective study of the incidence, clinical features, and outcome of symptomatic upper and lower respiratory tract infections by respiratory viruses in adult recipients of hematopoietic stem cell transplants for hematologic malignancies
    Rodrigo Martino
    Department of Clinical Hematology, Hospital de la Santa Creu i Sant Pau, and Autonomous University of Barcelona, Spain
    Biol Blood Marrow Transplant 11:781-96. 2005
    ..In addition, human metapneumovirus should be added to the potentially serious causes of RV infections in HSCT...
  69. ncbi request reprint Identification of an H-2D(b)-restricted CD8+ cytotoxic T lymphocyte epitope in the matrix protein of respiratory syncytial virus
    John A Rutigliano
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 2502, 40 Convent Drive, MSC 3017, Bethesda, MD 20892 3017, USA
    Virology 337:335-43. 2005
    ..Furthermore, identification of this H-2b-restricted CTL epitope provides access to genetically modified H-2b mice for more detailed studies of CTL mechanisms in RSV infection...
  70. pmc Human metapneumovirus infection in children hospitalized for wheezing
    John V Williams
    J Allergy Clin Immunol 115:1311-2. 2005
  71. pmc Respiratory syncytial virus nucleoprotein-specific cytotoxic T-cell epitopes in a South African population of diverse HLA types are conserved in circulating field strains
    Marietjie Venter
    National Institute for Communicable Diseases, Sandringham, South Africa
    J Virol 77:7319-29. 2003
    ..This work suggests that escape from CTL activity is not common for this acute respiratory infection...
  72. pmc Genetic basis for adverse events after smallpox vaccination
    David M Reif
    Computational Genetics Laboratory, Dartmouth Medical School, Lebanon, New Hampshire, USA
    J Infect Dis 198:16-22. 2008
    ....
  73. ncbi request reprint Genetic predisposition for adverse events after vaccination
    James E Crowe
    J Infect Dis 196:176-7. 2007

Research Grants10

  1. ONTOGENY OF RSV-SPECIFIC B CELL RESPONSES IN HUMANS
    James Crowe; Fiscal Year: 2001
    ..The data from this work will direct decisions about the best vaccine strategy to use against RSV in neonates, and will delineate basic mechanisms of the ontogeny of B-cell repertoire diversity in humans. ..
  2. HUMAN MONOCLONAL ANTIBODIES THAT NEUTRALIZE VACCINIA
    James Crowe; Fiscal Year: 2002
    ..In summary, this work will yield a safe and effective immunotherapeutic strategy for treatment of vaccinia complications. ..
  3. High Throughput Flow Cytometry Sorter
    James Crowe; Fiscal Year: 2004
    ..The requested instrument will meet all of these demonstrated needs. ..
  4. Immunomodulation on the Nanoscale (RMI)
    James Crowe; Fiscal Year: 2004
    ..Immunology and infectious diseases. 2. Materials science, especially nanoparticles, nanowires, and nanofluidics. 3. Instrumented platforms for cellular imaging and control. 4. Biocomputing. ..
  5. Novel methods for generation of human B cell hybridomas
    James Crowe; Fiscal Year: 2005
    ..abstract_text> ..
  6. Human T Cells Responses to RSV
    James Crowe; Fiscal Year: 2006
    ..abstract_text> ..
  7. Cell-Mediated Immune Responses to Vaccinia Viruses
    James Crowe; Fiscal Year: 2006
    ..We will examine many of these questions by taking advantage of new technology that allows for examination of T cell responses at the single cell level, and in an immunodominant epitope specific manner. [unreadable] [unreadable]..
  8. Human Antibody Responses to Rotavirus
    James Crowe; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  9. Alphavirus-based Vaccine for Prevention of MPV Infection
    James Crowe; Fiscal Year: 2008
    ..The consortium submitting this application brings together an experienced investigator group with expertise in pneumovirus and alphavirus virology, immunology, and vaccine development. ..
  10. Respiratory Syncytial Virus Nanoparticle Vaccines
    James Crowe; Fiscal Year: 2008
    ..The development of a new technology for vaccination using nanoparticles also could have wide impact, since such a vaccine technology might be useful in developing vaccines against other infections. [unreadable] [unreadable] [unreadable]..