WENDY CATHERINE BROWN

Summary

Affiliation: Washington State University
Country: USA

Publications

  1. ncbi request reprint Stimulation of B lymphocytes, macrophages, and dendritic cells by protozoan DNA
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Microbes Infect 4:969-74. 2002
  2. doi request reprint Persistent Infections and Immunity in Ruminants to Arthropod-Borne Bacteria in the Family Anaplasmataceae
    Wendy C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 email
    Annu Rev Anim Biosci 4:177-97. 2016
  3. pmc Loss of Immunization-Induced Epitope-Specific CD4 T-Cell Response following Anaplasma marginale Infection Requires Presence of the T-Cell Epitope on the Pathogen and Is Not Associated with an Increase in Lymphocytes Expressing Known Regulatory Cell Phenot
    Wendy C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 22:742-53. 2015
  4. pmc Immunoreactive Coxiella burnetii Nine Mile proteins separated by 2D electrophoresis and identified by tandem mass spectrometry
    James R Deringer
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    Microbiology 157:526-42. 2011
  5. pmc Adaptive immunity to Anaplasma pathogens and immune dysregulation: implications for bacterial persistence
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States
    Comp Immunol Microbiol Infect Dis 35:241-52. 2012
  6. pmc CD4 T cell antigens from Staphylococcus aureus Newman strain identified following immunization with heat-killed bacteria
    Paulraj K Lawrence
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 19:477-89. 2012
  7. ncbi request reprint Bovine type 1 and type 2 responses
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Vet Immunol Immunopathol 63:45-55. 1998
  8. pmc Association and evidence for linked recognition of type IV secretion system proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
    Kaitlyn Morse
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Paul G Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA
    Infect Immun 80:215-27. 2012
  9. ncbi request reprint Prospects for recombinant vaccines against Babesia bovis and related parasites
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Parasite Immunol 28:315-27. 2006
  10. ncbi request reprint Immune control of Babesia bovis infection
    Wendy C Brown
    Program in Vector borne Disease, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Vet Parasitol 138:75-87. 2006

Collaborators

Detail Information

Publications65

  1. ncbi request reprint Stimulation of B lymphocytes, macrophages, and dendritic cells by protozoan DNA
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Microbes Infect 4:969-74. 2002
    ..This novel mechanism of protozoan parasite recognition by the innate immune system could facilitate recovery from acute infection or contribute to infection-related pathology...
  2. doi request reprint Persistent Infections and Immunity in Ruminants to Arthropod-Borne Bacteria in the Family Anaplasmataceae
    Wendy C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 email
    Annu Rev Anim Biosci 4:177-97. 2016
    ..This indicates highly adapted strategies of Anaplasma spp. to both suppress protective immune responses and evade those that do develop. However, conserved subdominant antigens are potential targets for immunization. ..
  3. pmc Loss of Immunization-Induced Epitope-Specific CD4 T-Cell Response following Anaplasma marginale Infection Requires Presence of the T-Cell Epitope on the Pathogen and Is Not Associated with an Increase in Lymphocytes Expressing Known Regulatory Cell Phenot
    Wendy C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 22:742-53. 2015
    ..As hypothesized, the induction of T-cell exhaustion occurred only following infection with A. marginale, which did not correlate with an increase in either CD4(+) CD25(+) FoxP3(+) T cells or any γδ T-cell subset examined. ..
  4. pmc Immunoreactive Coxiella burnetii Nine Mile proteins separated by 2D electrophoresis and identified by tandem mass spectrometry
    James R Deringer
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    Microbiology 157:526-42. 2011
    ..The antigens described here warrant further investigation to validate their potential use as subunit vaccine candidates...
  5. pmc Adaptive immunity to Anaplasma pathogens and immune dysregulation: implications for bacterial persistence
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States
    Comp Immunol Microbiol Infect Dis 35:241-52. 2012
    ..marginale to that observed in other models of chronic infection resulting in high antigen loads, including malaria, a disease caused by another intraerythrocytic pathogen...
  6. pmc CD4 T cell antigens from Staphylococcus aureus Newman strain identified following immunization with heat-killed bacteria
    Paulraj K Lawrence
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 19:477-89. 2012
    ..These may be useful for inclusion in a broadly protective multiantigen vaccine against MRSA or IMI...
  7. ncbi request reprint Bovine type 1 and type 2 responses
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Vet Immunol Immunopathol 63:45-55. 1998
    ..In addition, we provide evidence that major regulatory cytokines, IL-4, IL-10, and IL-12, do not selectively exert their negative (IL-4 and IL-10) or positive (IL-12) effects on Th1-like cells...
  8. pmc Association and evidence for linked recognition of type IV secretion system proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
    Kaitlyn Morse
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Paul G Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA
    Infect Immun 80:215-27. 2012
    ..The immunogenicity and interactions of VirB9-1, VirB9-2, and VirB10 justify their testing as a linked protein vaccine against A. marginale...
  9. ncbi request reprint Prospects for recombinant vaccines against Babesia bovis and related parasites
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Parasite Immunol 28:315-27. 2006
    ..Development of successful non-living recombinant vaccines will depend on increased understanding of protective immune mechanisms and availability of parasite genomes...
  10. ncbi request reprint Immune control of Babesia bovis infection
    Wendy C Brown
    Program in Vector borne Disease, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Vet Parasitol 138:75-87. 2006
    ..The availability of an annotated B. bovis genome will, for the first time, enable identification of non-immunodominant proteins that may stimulate protective immunity...
  11. pmc CD4+ T lymphocytes from Anaplasma marginale major surface protein 2 (MSP2) vaccinees recognize naturally processed epitopes conserved in MSP3
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 72:3688-92. 2004
    ..This T-lymphocyte response to conserved and partially conserved epitopes may contribute to the immunodominance of MSP2 and MSP3...
  12. ncbi request reprint The hypervariable region of Anaplasma marginale major surface protein 2 (MSP2) contains multiple immunodominant CD4+ T lymphocyte epitopes that elicit variant-specific proliferative and IFN-gamma responses in MSP2 vaccinates
    Wendy C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    J Immunol 170:3790-8. 2003
    ..Antigenic variation in MSP2 Th cell and B cell epitopes may contribute to immune evasion that allows long-term persistence of A. marginale in the mammalian reservoir...
  13. pmc Major histocompatibility complex class II DR-restricted memory CD4(+) T lymphocytes recognize conserved immunodominant epitopes of Anaplasma marginale major surface protein 1a
    Wendy C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 70:5521-32. 2002
    ..The presence of multiple CD4(+) T-cell epitopes in the MSP1a HD, in addition to the neutralization-sensitive epitope, supports the testing of this immunogen for induction of protective immunity against A. marginale challenge...
  14. pmc CD4(+) T lymphocytes from calves immunized with Anaplasma marginale major surface protein 1 (MSP1), a heteromeric complex of MSP1a and MSP1b, preferentially recognize the MSP1a carboxyl terminus that is conserved among strains
    W C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 69:6853-62. 2001
    ..The data provide a basis for determining whether selected MSP1a CD4(+) T-lymphocyte epitopes and selected MSP1a and MSP1b B-lymphocyte epitopes presented on the same molecule can stimulate a protective immune response...
  15. ncbi request reprint Modulation of host immune responses by protozoal DNA
    W C Brown
    Washington State University, Department of Veterinary Microbiology and Pathology, Pullman 99164 7040, USA
    Vet Immunol Immunopathol 72:87-94. 1999
    ..These results also support the use of ISS as vaccine adjuvants to enhance Type 1 immune responses in cattle...
  16. ncbi request reprint Designing blood-stage vaccines against Babesia bovis and B. bigemina
    W C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA
    Parasitol Today 15:275-81. 1999
    ..In this article, Wendy Brown and Guy Palmer review strategies for identifying candidate vaccine antigens of B. bovis and B. bigemina and for priming immune responses to evoke strain crossprotective immunity...
  17. pmc Bovine CD4(+) T-lymphocyte clones specific for rhoptry-associated protein 1 of Babesia bigemina stimulate enhanced immunoglobulin G1 (IgG1) and IgG2 synthesis
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164, USA
    Infect Immun 67:155-64. 1999
    ..bigemina RAP-1 immune cattle and a biased IgG1 response in F. hepatica-immune cattle...
  18. pmc DNA and a CpG oligonucleotide derived from Babesia bovis are mitogenic for bovine B cells
    W C Brown
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 66:5423-32. 1998
    ..These ISS may be involved in host immune modulation during protozoal infection and may be useful as vaccine adjuvants...
  19. ncbi request reprint A novel 20-kilodalton protein conserved in Babesia bovis and B. bigemina stimulates memory CD4(+) T lymphocyte responses in B. bovis-immune cattle
    W C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, PO Box 647040, Pullman, WA 99164 7040, USA
    Mol Biochem Parasitol 118:97-109. 2001
    ..bovis strains and produced IFN-gamma. BLAST analysis revealed significant similarity of the Bbo20 and Bbg20 amino acid sequences with the hsp20/alpha-crystallin family...
  20. pmc The repertoire of Anaplasma marginale antigens recognized by CD4(+) T-lymphocyte clones from protectively immunized cattle is diverse and includes major surface protein 2 (MSP-2) and MSP-3
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 66:5414-22. 1998
    ..Our results provide a rationale for identifying Th cell epitopes conserved among different strains of A. marginale for inclusion in a nucleic acid or recombinant protein vaccine...
  21. pmc CD4(+) T-lymphocyte and immunoglobulin G2 responses in calves immunized with Anaplasma marginale outer membranes and protected against homologous challenge
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 66:5406-13. 1998
    ....
  22. pmc Helper T-cell epitopes encoded by the Babesia bigemina rap-1 gene family in the constant and variant domains are conserved among parasite strains
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Infect Immun 66:1561-9. 1998
    ..The immunogenic and highly conserved nature of these T-cell epitopes and their ability to stimulate functionally relevant Th cells that express gamma interferon support their inclusion in a vaccine...
  23. ncbi request reprint Immunodominant T-cell antigens and epitopes of Babesia bovis and Babesia bigemina
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164, USA
    Ann Trop Med Parasitol 92:473-82. 1998
    ..bigemina is the subject of the present review...
  24. ncbi request reprint Molecular approaches to elucidating innate and acquired immune responses to Babesia bovis, a protozoan parasite that causes persistent infection
    W C Brown
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Vet Parasitol 101:233-48. 2001
    ....
  25. ncbi request reprint Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale
    Jeffrey R Abbott
    Program in Vector borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164, USA
    J Immunol 174:6702-15. 2005
    ..marginale organisms. These results suggest that loss of memory T cell responses following A. marginale infection is due to a mechanism other than induction of T regulatory cells, such as peripheral deletion of MSP2-specific T cells...
  26. pmc Stimulation of T-helper cell gamma interferon and immunoglobulin G responses specific for Babesia bovis rhoptry-associated protein 1 (RAP-1) or a RAP-1 protein lacking the carboxy-terminal repeat region is insufficient to provide protective immunity again
    Junzo Norimine
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 71:5021-32. 2003
    ..We conclude that successful priming of calves with recombinant RAP-1 and adjuvants that elicit strong Th1 cell and IgG responses is insufficient to protect calves against virulent B. bovis challenge...
  27. pmc Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 75:2333-42. 2007
    ..marginale and related pathogens...
  28. ncbi request reprint CpG ODN 2006 and IL-12 are comparable for priming Th1 lymphocyte and IgG responses in cattle immunized with a rickettsial outer membrane protein in alum
    Yan Zhang
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164 7040, USA
    Vaccine 21:3307-18. 2003
    ..No differences in IL-10 mRNA expression were detected among the groups. These results indicate that CpG ODN 2006 is an effective vaccine adjuvant for stimulating both antibody and IFN-gamma mediated cellular immune responses in cattle...
  29. pmc Conservation of Babesia bovis small heat shock protein (Hsp20) among strains and definition of T helper cell epitopes recognized by cattle with diverse major histocompatibility complex class II haplotypes
    Junzo Norimine
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 72:1096-106. 2004
    ....
  30. pmc Identification of vaccine candidate peptides in the NcSRS2 surface protein of Neospora caninum by using CD4+ cytotoxic T lymphocytes and gamma interferon-secreting T lymphocytes of infected holstein cattle
    Lauren M Staska
    Department of Veterinary Microbiology and Pathology, Bustad Hall, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 73:1321-9. 2005
    ..These findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences or peptides for induction of NcSRS2 peptide-specific CTL and IFN-gamma-secreting T lymphocytes in cattle with varied MHC genotypes...
  31. pmc Rapid deletion of antigen-specific CD4+ T cells following infection represents a strategy of immune evasion and persistence for Anaplasma marginale
    Sushan Han
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    J Immunol 181:7759-69. 2008
    ..Thus, infection of cattle with A. marginale leads to the rapid loss of Ag-specific T cells and immunologic memory, which may be a strategy for this pathogen to modulate the immune response and persist...
  32. pmc Immunodominant epitopes in Babesia bovis rhoptry-associated protein 1 that elicit memory CD4(+)-T-lymphocyte responses in B. bovis-immune individuals are located in the amino-terminal domain
    Junzo Norimine
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology Animal Disease Research Unit, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 70:2039-48. 2002
    ..The presence of T-helper-cell epitopes in the NT domain of RAP-1, which is highly conserved among otherwise antigenically different strains of B. bovis, supports the inclusion of this region in vaccine constructs to be tested in cattle...
  33. pmc Physical linkage of naturally complexed bacterial outer membrane proteins enhances immunogenicity
    Henriette Macmillan
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    Infect Immun 76:1223-9. 2008
    ..Understanding how the naturally occurring intermolecular interactions between OMPs influence the immune response may lead to more effective vaccine design...
  34. pmc Anaplasma marginale major surface protein 2 CD4+-T-cell epitopes are evenly distributed in conserved and hypervariable regions (HVR), whereas linear B-cell epitopes are predominantly located in the HVR
    Jeffrey R Abbott
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    Infect Immun 72:7360-6. 2004
    ..The pattern of recognition of epitope clusters by T cells and of HVR epitopes by B cells is consistent with the influence of protein structure on epitope recognition...
  35. doi request reprint High-throughput identification of T-lymphocyte antigens from Anaplasma marginale expressed using in vitro transcription and translation
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States
    J Immunol Methods 332:129-41. 2008
    ..This method allows rapid expression and identification of T-lymphocyte antigens for any pathogen for which the genome sequence is available...
  36. pmc DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals
    Waithaka Mwangi
    Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Clin Vaccine Immunol 14:304-11. 2007
    ..These results demonstrate that DNA vaccines targeting key intercellular and intracellular events significantly enhance priming and expansion and support the feasibility of single-dose DNA immunization in outbred populations...
  37. pmc Maintenance of antibody to pathogen epitopes generated by segmental gene conversion is highly dynamic during long-term persistent infection
    Yan Zhuang
    Program in Vector Borne Diseases and Immunology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 75:5185-90. 2007
    ..The results unify the genetic potential of segmental gene conversion with escape from antibody recognition and identify immunological effects of variant mosaic structure...
  38. ncbi request reprint A novel 78-kDa fatty acyl-CoA synthetase (ACS1) of Babesia bovis stimulates memory CD4+ T lymphocyte responses in B. bovis-immune cattle
    Junzo Norimine
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Mol Biochem Parasitol 147:20-9. 2006
    ..However, immune sera from cattle recovered from B. bovis infection did not react with ACS1, indicating that epitopes may be conformationally dependent...
  39. pmc Identification of novel antigenic proteins in a complex Anaplasma marginale outer membrane immunogen by mass spectrometry and genomic mapping
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 73:8109-18. 2005
    ..The identification of these novel antigenic proteins markedly expands current understanding of the composition of the protective immunogen and provides new candidates for vaccine development...
  40. pmc Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine
    Eric L Sutten
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 78:1314-25. 2010
    ..The discovery of novel immunogenic T4SS proteins recognized by outbred individuals with common MHC haplotypes further justifies evaluating the T4SS as a potential vaccine candidate for pathogenic bacteria...
  41. pmc Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge
    Darrilyn G Fraser
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Clin Diagn Lab Immunol 12:983-93. 2005
    ..Thus, lipopeptide-induced proliferative responses and enhanced Th and CTL responses early after virus challenge were unable to control challenge virus load and clinical disease...
  42. ncbi request reprint The CD4+ T cell immunodominant Anaplasma marginale major surface protein 2 stimulates gammadelta T cell clones that express unique T cell receptors
    Kevin K Lahmers
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Leukoc Biol 77:199-208. 2005
    ..Together, these data indicate that WC1+ gammadelta T cells recognize A. marginale MSP2 through the TCR and contribute to the immunodominant response to this protein...
  43. pmc Expression of Anaplasma marginale major surface protein 2 operon-associated proteins during mammalian and arthropod infection
    CHRISTIANE V LOHR
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 70:6005-12. 2002
    ....
  44. pmc The Babesia bovis merozoite surface antigen 2 locus contains four tandemly arranged and expressed genes encoding immunologically distinct proteins
    Monica Florin-Christensen
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 70:3566-75. 2002
    ..The results support the evolution of the msa-2 locus through at least two gene duplications, with selection for multiple related but antigenically distinct merozoite surface proteins...
  45. pmc The immunization-induced antibody response to the Anaplasma marginale major surface protein 2 and its association with protective immunity
    Susan M Noh
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, WA 99164 7030, USA
    Vaccine 28:3741-7. 2010
    ....
  46. pmc Composition of the surface proteome of Anaplasma marginale and its role in protective immunity induced by outer membrane immunization
    Susan M Noh
    Animal Disease Research Unit, Agriculture Research Service, U S Department of Agriculture, Pullman, WA 99164 7030, USA
    Infect Immun 76:2219-26. 2008
    ..Furthermore, the data support that remodeling of the surface proteome accompanies the transition between mammalian and arthropod hosts and identify novel targets for blocking transmission...
  47. doi request reprint CD8(+)/perforin(+)/WC1(-) gammadelta T cells, not CD8(+) alphabeta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever
    Danielle D Nelson
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA
    Vet Immunol Immunopathol 136:284-91. 2010
    ..Results of the present study support the previous suggestions that MCF is fundamentally a disease of immune dysregulation...
  48. pmc Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa
    Kelly A Brayton
    Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    PLoS Pathog 3:1401-13. 2007
    ..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
  49. pmc Differential expression and sequence conservation of the Anaplasma marginale msp2 gene superfamily outer membrane proteins
    Susan M Noh
    Program in Vector borne Diseas, Department of Microbiology and Pathology, Washington State University, Pullman, WA 99164 6630, USA
    Infect Immun 74:3471-9. 2006
    ..Maries strain and Florida strain genomes...
  50. pmc Characterization of a phenotypically unique population of CD13+ dendritic cells resident in the spleen
    Yan Zhuang
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164 7040, USA
    Clin Vaccine Immunol 13:1064-9. 2006
    ..Upon cytokine-induced maturation, splenic DCs both efficiently present antigen in the stimulation of allogeneic lymphocyte proliferation and recall antigen-specific responses...
  51. ncbi request reprint Quantitation of Anaplasma marginale major surface protein (MSP)1a and MSP2 epitope-specific CD4+ T lymphocytes using bovine DRB3*1101 and DRB3*1201 tetramers
    Junzo Norimine
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Immunogenetics 58:726-39. 2006
    ..This novel technology should be useful to track the fate of antigen-specific CD4+ T-cell responses in cattle after immunization or infection with persistent pathogens, such as A. marginale, that modulate the host immune response...
  52. pmc Analysis of the Anaplasma marginale major surface protein 1 complex protein composition by tandem mass spectrometry
    Henriette Macmillan
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Bacteriol 188:4983-91. 2006
    ..The msp1beta sequences were highly conserved throughout infection. However, liquid chromatography-tandem mass spectrometry analysis identified only a single MSP1b protein, MSP1b1, within the MSP1 complex...
  53. ncbi request reprint Bovine splenic NK cells synthesize IFN-gamma in response to IL-12-containing supernatants from Babesia bovis-exposed monocyte cultures
    Will L Goff
    Animal Disease Research Unit, USDA ARS, Pullman, Washington, USA
    Parasite Immunol 28:221-8. 2006
    ..Finally, innate IFN-gamma production was induced in splenic NK cells in the presence of supernatants from B. bovis merozoite-exposed monocytes in an IL-12 pathway-dependent manner...
  54. ncbi request reprint Genomic and proteomic approaches to vaccine candidate identification for Anaplasma marginale
    Kelly A Brayton
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99163 7040, USA
    Expert Rev Vaccines 5:95-101. 2006
    ..These data were mapped back to the annotated A. marginale genome and have identified several new outer membrane proteins that are vaccine candidates...
  55. ncbi request reprint Identification and phylogenetic analysis of 15 MHC class II DRB1 beta1 expressed alleles in a ewe-lamb flock
    Lynn M Herrmann
    Animal Disease Research Unit, USDA ARS, 3003 ADBF, Washington State University, Pullman, 99164 6630, USA
    Immunogenetics 57:855-63. 2005
    ..Phylogenetic analysis of the 15 Ovar-DRB1 beta1 cDNA sequences revealed that DRB1*0206 had a strong phylogenetic relationship to DRB1*0203, and DRB1*0353 had a strong phylogenetic relationship to DRB1*0303...
  56. ncbi request reprint Intrahaplotype and interhaplotype pairing of bovine leukocyte antigen DQA and DQB molecules generate functional DQ molecules important for priming CD4(+) T-lymphocyte responses
    Junzo Norimine
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Immunogenetics 57:750-62. 2005
    ..Functional BoLA-DQ molecules are generated by both intrahaplotype and interhaplotype pairing of A and B chains and play a similar role to BoLA-DR in priming helper T-cell responses to important pathogens...
  57. ncbi request reprint Enhancement of antigen acquisition by dendritic cells and MHC class II-restricted epitope presentation to CD4+ T cells using VP22 DNA vaccine vectors that promote intercellular spreading following initial transfection
    Waithaka Mwangi
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Leukoc Biol 78:401-11. 2005
    ....
  58. pmc Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle
    Will L Goff
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 6630, USA
    Clin Diagn Lab Immunol 10:38-43. 2003
    ..3% concordance). The assay appears to have the characteristics necessary for an epidemiologic and disease surveillance tool...
  59. ncbi request reprint DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred anim
    Waithaka Mwangi
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    J Immunol 169:3837-46. 2002
    ..These results support use of these growth factors in DNA vaccination and specifically indicate their applicability for vaccine testing in outbred animals...
  60. pmc Selective in vivo depletion of CD4(+) T lymphocytes with anti-CD4 monoclonal antibody during acute infection of calves with Anaplasma marginale
    Reginald A Valdez
    Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Pullman, Washington 99163, USA
    Clin Diagn Lab Immunol 9:417-24. 2002
    ..This was in contrast to the prediction that significant depletion of CD4(+) T lymphocytes would abrogate resistance to acute infection...
  61. pmc Identification of broadly recognized, T helper 1 lymphocyte epitopes in an equine lentivirus
    Darrilyn G Fraser
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Immunology 105:295-305. 2002
    ..Finally, CD4(+) T lymphocytes were required for proliferation responses, as shown by assays using CD4- versus CD8-depleted PBMC...
  62. ncbi request reprint Comparative gene expression by WC1+ gammadelta and CD4+ alphabeta T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ gammadelta T cells
    Kevin K Lahmers
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Leukoc Biol 80:939-52. 2006
    ..It is important that many of these genes were also expressed at higher levels in polyclonal WC1(+) gammadelta T cells when compared with CD4(+) alphabeta T cells selected from peripheral blood...
  63. doi request reprint Molecular cloning of CD18 of bison, deer and elk, and comparison with that of other ruminants and non-ruminants
    Sudarvili Shanthalingam
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Vet Immunol Immunopathol 136:163-9. 2010
    ..Phylogenetic analysis based on amino acid sequences showed that CD18 of bison is most closely related to that of cattle while CD18 of deer and elk are more closely related to each other...
  64. pmc The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion
    Tanya LeRoith
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 74:3663-7. 2006
    ..The results indicate that the MSA-1 HVR is involved in erythrocyte invasion and suggest that selection of MSA-1 variants may be driven by invasion-blocking antibodies...
  65. pmc Anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory CD4+ T lymphocyte response but sustained high IgG titers
    Sushan Han
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Clin Vaccine Immunol 17:1881-90. 2010
    ..marginale infection. The failure to establish a strong memory T cell response during A. marginale infection likely contributes to bacterial persistence...