C E Suarez

Summary

Affiliation: Washington State University
Country: USA

Publications

  1. doi request reprint Integration of a transfected gene into the genome of Babesia bovis occurs by legitimate homologous recombination mechanisms
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164 6630, United States Electronic address
    Mol Biochem Parasitol 202:23-8. 2015
  2. pmc One Health approach to identify research needs in bovine and human babesioses: workshop report
    Adalberto A Perez de Leon
    Knipling Bushland U, S, Livestock Insects Research Laboratory, USDA ARS, Kerrville, TX, USA
    Parasit Vectors 3:36. 2010
  3. pmc Comparative genomic analysis and phylogenetic position of Theileria equi
    Lowell S Kappmeyer
    Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA 99164 7030, USA
    BMC Genomics 13:603. 2012
  4. doi request reprint Acute and persistent infection by a transfected Mo7 strain of Babesia bovis
    Carlos E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 704, United States
    Mol Biochem Parasitol 185:52-7. 2012
  5. pmc Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis
    Maria Mesplet
    Instituto de Patobiologia, Centro de Investigaciones en Ciencias Veterinarias y Agronomicas, Instituto Nacional de Tecnologia Agropecuaria, INTA Castelar, Argentina
    Parasit Vectors 3:113. 2010
  6. ncbi request reprint Intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in Babesia bovis
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Int J Parasitol 34:1177-84. 2004
  7. ncbi request reprint Characterization and gene expression of Babesia bovis elongation factor-1alpha
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Int J Parasitol 36:965-73. 2006
  8. ncbi request reprint Optimization of Babesia bovis transfection methods
    C E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Parassitologia 49:67-70. 2007
  9. ncbi request reprint Transient transfection of purified Babesia bovis merozoites
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Exp Parasitol 118:498-504. 2008
  10. doi request reprint Stable expression of a GFP-BSD fusion protein in Babesia bovis merozoites
    Carlos E Suarez
    Program in Vector Borne Diseases, Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164 7040, USA
    Int J Parasitol 39:289-97. 2009

Collaborators

Detail Information

Publications40

  1. doi request reprint Integration of a transfected gene into the genome of Babesia bovis occurs by legitimate homologous recombination mechanisms
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164 6630, United States Electronic address
    Mol Biochem Parasitol 202:23-8. 2015
    ..Use of legitimate homologous recombination mechanisms in transfected B. bovis supports the future use of transfection methods for developing efficient gene function assignment experiments using gene knockout techniques. ..
  2. pmc One Health approach to identify research needs in bovine and human babesioses: workshop report
    Adalberto A Perez de Leon
    Knipling Bushland U, S, Livestock Insects Research Laboratory, USDA ARS, Kerrville, TX, USA
    Parasit Vectors 3:36. 2010
    ..abstract:..
  3. pmc Comparative genomic analysis and phylogenetic position of Theileria equi
    Lowell S Kappmeyer
    Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA 99164 7030, USA
    BMC Genomics 13:603. 2012
    ..equi relative to sequenced apicomplexan parasites...
  4. doi request reprint Acute and persistent infection by a transfected Mo7 strain of Babesia bovis
    Carlos E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 704, United States
    Mol Biochem Parasitol 185:52-7. 2012
    ..Together, the data demonstrates that exogenous B. bovis transgenes can be expressed and remain stable throughout acute and persistent infection in calves...
  5. pmc Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis
    Maria Mesplet
    Instituto de Patobiologia, Centro de Investigaciones en Ciencias Veterinarias y Agronomicas, Instituto Nacional de Tecnologia Agropecuaria, INTA Castelar, Argentina
    Parasit Vectors 3:113. 2010
    ..abstract:..
  6. ncbi request reprint Intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in Babesia bovis
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Int J Parasitol 34:1177-84. 2004
    ..These results are the first to demonstrate exogenous gene expression in B. bovis after transfection, and to confirm that the B. bovisrap-1 IG region can promote extra-chromosomal gene expression in vivo...
  7. ncbi request reprint Characterization and gene expression of Babesia bovis elongation factor-1alpha
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Int J Parasitol 36:965-73. 2006
    ..Interestingly, in contrast to other related intra-erythrocytic apicomplexans, the ef-1alpha locus of B. bovis contains a 160 bp intron in the 5' untranslated region...
  8. ncbi request reprint Optimization of Babesia bovis transfection methods
    C E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Parassitologia 49:67-70. 2007
    ..This information will facilitate further development of efficient stable transfection systems...
  9. ncbi request reprint Transient transfection of purified Babesia bovis merozoites
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Exp Parasitol 118:498-504. 2008
    ..bovis merozoites using either nucleofection or conventional electroporation. However, nucleofection is significantly more efficient when transfecting either circular or restriction digested DNA in the 2-10 microg range...
  10. doi request reprint Stable expression of a GFP-BSD fusion protein in Babesia bovis merozoites
    Carlos E Suarez
    Program in Vector Borne Diseases, Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164 7040, USA
    Int J Parasitol 39:289-97. 2009
    ..bovis, one of the three major tick-borne parasites that limit global livestock production...
  11. doi request reprint Transfection systems for Babesia bovis: a review of methods for the transient and stable expression of exogenous genes
    Carlos E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Vet Parasitol 167:205-15. 2010
    ..bovis strains. Further development of these transfection systems will facilitate functional analysis of B. bovis genes and will improve our understanding of the biology of and immunological response to this parasite...
  12. doi request reprint Emerging perspectives in the research of bovine babesiosis and anaplasmosis
    Carlos E Suarez
    Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164 6630, USA
    Vet Parasitol 180:109-25. 2011
    ....
  13. ncbi request reprint Organization, transcription, and expression of rhoptry associated protein genes in the Babesia bigemina rap-1 locus
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Mol Biochem Parasitol 127:101-12. 2003
    ..Immunoblot analysis of merozoites revealed the expected RAP-1a expression but failed to detect expressed RAP-1b and RAP-1c, indicating that expression of the rap-1 genes is regulated both at the transcriptional and translational levels...
  14. ncbi request reprint Structure, sequence, and transcriptional analysis of the Babesia bovis rap-1 multigene locus
    C E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Mol Biochem Parasitol 93:215-24. 1998
    ..bigemina versus B. bovis are reflected in the marked structural and antigenic polymorphism in the B. bigemina RAP-1 molecules as compared with the essentially monomorphic RAP-1 in B. bovis...
  15. ncbi request reprint The use of monoclonal antibodies with colloidal gold-labeled probes in postembedding immunoelectron microscopy
    C E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Mol Biotechnol 7:145-51. 1997
    ..In this article, we discuss critical technical aspects that usually result in improved resolution when the procedure is used to identify structure in diverse prokaryotic and eukaryotic cells...
  16. ncbi request reprint Sequence and functional analysis of the intergenic regions separating babesial rhoptry-associated protein-1 (rap-1) genes
    C E Suarez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164 7040, USA
    Exp Parasitol 90:189-94. 1998
    ..The presence of conserved regions 5' to each rap-1 gene copy and among other babesial rap-1 IG regions and the in vitro promoter function in the 5' --> 3' orientation support a role for the IG region in rap-1 gene regulation...
  17. pmc Characterization of allelic variation in the Babesia bovis merozoite surface antigen 1 (MSA-1) locus and identification of a cross-reactive inhibition-sensitive MSA-1 epitope
    C E Suarez
    Program in Vector Borne Diseases, Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Washington 99164, USA
    Infect Immun 68:6865-70. 2000
    ....
  18. ncbi request reprint A novel 20-kilodalton protein conserved in Babesia bovis and B. bigemina stimulates memory CD4(+) T lymphocyte responses in B. bovis-immune cattle
    W C Brown
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, PO Box 647040, Pullman, WA 99164 7040, USA
    Mol Biochem Parasitol 118:97-109. 2001
    ..bovis strains and produced IFN-gamma. BLAST analysis revealed significant similarity of the Bbo20 and Bbg20 amino acid sequences with the hsp20/alpha-crystallin family...
  19. pmc DNA and a CpG oligonucleotide derived from Babesia bovis are mitogenic for bovine B cells
    W C Brown
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 66:5423-32. 1998
    ..These ISS may be involved in host immune modulation during protozoal infection and may be useful as vaccine adjuvants...
  20. pmc DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide
    L K Shoda
    Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 69:2162-71. 2001
    ..The recognition of protozoal DNA by B lymphocytes and macrophages may provide an important innate defense mechanism to control parasite replication and promote persistent infection...
  21. ncbi request reprint Genetic variation in the dimorphic regions of RAP-1 genes and rap-1 loci of Babesia bigemina
    I Hotzel
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Mol Biochem Parasitol 90:479-89. 1997
    ..Collectively, the data suggest that gene conversion and unequal recombination events contribute to overall rap-1 sequence conservation among gene variants and strains but may also generate new rap-1 variants...
  22. pmc Helper T-cell epitopes encoded by the Babesia bigemina rap-1 gene family in the constant and variant domains are conserved among parasite strains
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Infect Immun 66:1561-9. 1998
    ..The immunogenic and highly conserved nature of these T-cell epitopes and their ability to stimulate functionally relevant Th cells that express gamma interferon support their inclusion in a vaccine...
  23. ncbi request reprint Immunodominant T-cell antigens and epitopes of Babesia bovis and Babesia bigemina
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164, USA
    Ann Trop Med Parasitol 92:473-82. 1998
    ..bigemina is the subject of the present review...
  24. ncbi request reprint Modulation of host immune responses by protozoal DNA
    W C Brown
    Washington State University, Department of Veterinary Microbiology and Pathology, Pullman 99164 7040, USA
    Vet Immunol Immunopathol 72:87-94. 1999
    ..These results also support the use of ISS as vaccine adjuvants to enhance Type 1 immune responses in cattle...
  25. ncbi request reprint Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis
    J Florin-Christensen
    Department of Veterinary Microbiology and Pathology, Washington State University WSU, Pullman 99164 7040, USA
    Mol Biochem Parasitol 106:147-56. 2000
    ..The striking differences in the contents of phosphatidylcholine between host erythrocytes and the parasite suggests that it may be a useful target for both chemotherapy and immunoprophylaxis against bovine babesiosis...
  26. ncbi request reprint Prospects for recombinant vaccines against Babesia bovis and related parasites
    W C Brown
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Parasite Immunol 28:315-27. 2006
    ..Development of successful non-living recombinant vaccines will depend on increased understanding of protective immune mechanisms and availability of parasite genomes...
  27. pmc In vivo binding of immunoglobulin M to the surfaces of Babesia bigemina-infected erythrocytes
    I E Echaide
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 66:2922-7. 1998
    ..The common feature of IgM binding to the parasitized erythrocyte surface among otherwise genetically and antigenically distinct B. bigemina strains is suggestive of an advantageous role in parasite survival in vivo...
  28. ncbi request reprint Babesia bigemina: isolation and characterization of merozoite rhoptries
    R Z Machado
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040
    Exp Parasitol 77:315-25. 1993
    ....
  29. doi request reprint Babesia bovis: transcriptional analysis of rRNA gene unit expression
    Jacob M Laughery
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Exp Parasitol 123:45-50. 2009
    ..Collectively, the data indicate that differential transcription of rRNA occurs in B.bovis, depending on the life stage of the parasite and on the environment...
  30. pmc A unique phospholipid organization in bovine erythrocyte membranes
    J Florin-Christensen
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA
    Proc Natl Acad Sci U S A 98:7736-41. 2001
    ....
  31. pmc The Babesia bovis merozoite surface antigen 2 locus contains four tandemly arranged and expressed genes encoding immunologically distinct proteins
    Monica Florin-Christensen
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 70:3566-75. 2002
    ..The results support the evolution of the msa-2 locus through at least two gene duplications, with selection for multiple related but antigenically distinct merozoite surface proteins...
  32. pmc Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa
    Kelly A Brayton
    Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    PLoS Pathog 3:1401-13. 2007
    ..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
  33. pmc Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle
    Will L Goff
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 6630, USA
    Clin Diagn Lab Immunol 10:38-43. 2003
    ..3% concordance). The assay appears to have the characteristics necessary for an epidemiologic and disease surveillance tool...
  34. pmc Validation of a competitive enzyme-linked immunosorbent assay for detection of antibodies against Babesia bovis
    Will L Goff
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 6630, USA
    Clin Vaccine Immunol 13:1212-6. 2006
    ..Analysis of variance of the resulting optical densities and a test of homogeneity indicated no significant difference among the laboratories. Overall, the cELISA appears to have the attributes necessary for international application...
  35. ncbi request reprint Neospora caninum: antibodies directed against tachyzoite surface protein NcSRS2 inhibit parasite attachment and invasion of placental trophoblasts in vitro
    Gary J Haldorson
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Exp Parasitol 112:172-8. 2006
    ..2.4.4 and monospecific serum. The findings provide rationale to investigate a role for antibodies to NcSRS2 in prevention of N. caninum transplacental transmission in vivo...
  36. ncbi request reprint Fewer PrPc myeloid-based cells in sheep with the prion-resistant genotype
    Lynn M Herrmann
    Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, USA
    Neuroreport 17:125-9. 2006
    ..05). One possibility for the lack of PrP(Sc) accumulation in brains and lymph nodes of scrapie-exposed sheep with 171RR PRNP genetics is because of fewer PrPc-positive myeloid-derived cells available for conversion of PrPc to PrP(Sc)...
  37. pmc Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle
    Will L Goff
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 6630, USA
    Clin Vaccine Immunol 15:1316-21. 2008
    ..8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application...
  38. ncbi request reprint Immunostimulatory CpG-modified plasmid DNA enhances IL-12, TNF-alpha, and NO production by bovine macrophages
    L K Shoda
    Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, Washington 99164-7040, USA
    J Leukoc Biol 70:103-12. 2001
    ..Engineered CpG-pDNA or CpG-oligodeoxynucleotides should be useful as vaccines or adjuvants to promote the enhanced type 1 responses important for protection against intracellular pathogens...
  39. pmc Immunodominant epitopes in Babesia bovis rhoptry-associated protein 1 that elicit memory CD4(+)-T-lymphocyte responses in B. bovis-immune individuals are located in the amino-terminal domain
    Junzo Norimine
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology Animal Disease Research Unit, Washington State University, Pullman, Washington 99164, USA
    Infect Immun 70:2039-48. 2002
    ..The presence of T-helper-cell epitopes in the NT domain of RAP-1, which is highly conserved among otherwise antigenically different strains of B. bovis, supports the inclusion of this region in vaccine constructs to be tested in cattle...
  40. pmc Identification of vaccine candidate peptides in the NcSRS2 surface protein of Neospora caninum by using CD4+ cytotoxic T lymphocytes and gamma interferon-secreting T lymphocytes of infected holstein cattle
    Lauren M Staska
    Department of Veterinary Microbiology and Pathology, Bustad Hall, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 73:1321-9. 2005
    ..These findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences or peptides for induction of NcSRS2 peptide-specific CTL and IFN-gamma-secreting T lymphocytes in cattle with varied MHC genotypes...