Xiaowei Wang

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
    Athanasia Spandidos
    Center for Computational and Integrative Biology, Massachusetts General Hospital, MA, USA
    BMC Genomics 9:633. 2008
  2. ncbi request reprint Prediction of both conserved and nonconserved microRNA targets in animals
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioinformatics 24:325-32. 2008
  3. pmc miRDB: a microRNA target prediction and functional annotation database with a wiki interface
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    RNA 14:1012-7. 2008
  4. pmc A PCR-based platform for microRNA expression profiling studies
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri 63108, USA
    RNA 15:716-23. 2009
  5. pmc Selection of hyperfunctional siRNAs with improved potency and specificity
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63108, USA
    Nucleic Acids Res 37:e152. 2009
  6. pmc A novel RT-PCR method for quantification of human papillomavirus transcripts in archived tissues and its application in oropharyngeal cancer prognosis
    Ge Gao
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63108, USA
    Int J Cancer 132:882-90. 2013
  7. doi request reprint Verrucous carcinomas of the head and neck, including those with associated squamous cell carcinoma, lack transcriptionally active high-risk human papillomavirus
    Kalyani R Patel
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Pathol 44:2385-92. 2013
  8. pmc A microRNA expression signature for cervical cancer prognosis
    Xiaoxia Hu
    Departments of Radiation Oncology, Pathology and Immunology, and Obstetrics and Gynecology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 70:1441-8. 2010
  9. pmc Detection and significance of human papillomavirus, CDKN2A(p16) and CDKN1A(p21) expression in squamous cell carcinoma of the larynx
    Rebecca D Chernock
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mod Pathol 26:223-31. 2013
  10. pmc A microRNA expression signature for the prognosis of oropharyngeal squamous cell carcinoma
    Ge Gao
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri, USA
    Cancer 119:72-80. 2013

Collaborators

Detail Information

Publications28

  1. pmc A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
    Athanasia Spandidos
    Center for Computational and Integrative Biology, Massachusetts General Hospital, MA, USA
    BMC Genomics 9:633. 2008
    ....
  2. ncbi request reprint Prediction of both conserved and nonconserved microRNA targets in animals
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioinformatics 24:325-32. 2008
    ..Animal miRNAs have limited sequence complementarity to their gene targets, which makes it challenging to build target prediction models with high specificity...
  3. pmc miRDB: a microRNA target prediction and functional annotation database with a wiki interface
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    RNA 14:1012-7. 2008
    ..This is a new attempt to develop an interactive community-annotated miRNA functional catalog. All data stored in miRDB are freely accessible at http://mirdb.org...
  4. pmc A PCR-based platform for microRNA expression profiling studies
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri 63108, USA
    RNA 15:716-23. 2009
    ..The primer design algorithm has been extensively validated experimentally and has been shown to have robust performance for the measurement of miRNA expression in a variety of biological samples at a low cost...
  5. pmc Selection of hyperfunctional siRNAs with improved potency and specificity
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63108, USA
    Nucleic Acids Res 37:e152. 2009
    ..The siRNA design web server is available at http://www5.appliedbiosystems.com/tools/siDesign/...
  6. pmc A novel RT-PCR method for quantification of human papillomavirus transcripts in archived tissues and its application in oropharyngeal cancer prognosis
    Ge Gao
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63108, USA
    Int J Cancer 132:882-90. 2013
    ..Thus, p16 mRNA or protein expression signature is a sensitive and specific surrogate marker for HPV transcriptional activity (all genotypes combined)...
  7. doi request reprint Verrucous carcinomas of the head and neck, including those with associated squamous cell carcinoma, lack transcriptionally active high-risk human papillomavirus
    Kalyani R Patel
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Pathol 44:2385-92. 2013
    ..These findings argue that verrucous carcinoma and its related squamous cell carcinomas are not HPV-driven tumors. ..
  8. pmc A microRNA expression signature for cervical cancer prognosis
    Xiaoxia Hu
    Departments of Radiation Oncology, Pathology and Immunology, and Obstetrics and Gynecology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 70:1441-8. 2010
    ..In particular, miR-200a is likely to affect the metastatic potential of cervical cancer cells by coordinate suppression of multiple genes controlling cell motility...
  9. pmc Detection and significance of human papillomavirus, CDKN2A(p16) and CDKN1A(p21) expression in squamous cell carcinoma of the larynx
    Rebecca D Chernock
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mod Pathol 26:223-31. 2013
    ..Because of this, CDKN2A(p16) should not be used as a definitive surrogate marker of HPV-driven tumors in the larynx...
  10. pmc A microRNA expression signature for the prognosis of oropharyngeal squamous cell carcinoma
    Ge Gao
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri, USA
    Cancer 119:72-80. 2013
    ..One important goal is to identify those patients who are likely to fail standard therapy and who could potentially benefit from alternative or targeted treatments...
  11. pmc Prognostic microRNA signatures derived from The Cancer Genome Atlas for head and neck squamous cell carcinomas
    Nathan Wong
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri
    Cancer Med 5:1619-28. 2016
    ..This demonstrates the power of using TCGA as a potential resource to develop prognostic tools for improving individualized patient care. ..
  12. pmc Activation of miR-9 by human papillomavirus in cervical cancer
    Weijun Liu
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri
    Oncotarget 5:11620-30. 2014
    ..Thus, our work helps to understand the molecular mechanisms as well as identify potential therapeutic targets for cervical cancer and other HPV-induced cancers. ..
  13. doi request reprint Computational prediction of microRNA targets
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO, USA
    Methods Mol Biol 667:283-95. 2010
    ..In addition, a wiki editing interface has been established to allow anyone with Internet access to make contributions on miRNA functional annotation. All data stored in miRDB are freely accessible at http://www.mirdb.org...
  14. pmc Pseudohypoxia induced by miR-126 deactivation promotes migration and therapeutic resistance in renal cell carcinoma
    Weijun Liu
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO, USA
    Cancer Lett 394:65-75. 2017
    ..Finally, the clinical relevance of miR-126 modulated gene regulation in ccRCC has been confirmed with profiling data from The Cancer Genome Atlas...
  15. doi request reprint Histologic Typing in Oropharyngeal Squamous Cell Carcinoma: A 4-Year Prospective Practice Study With p16 and High-Risk HPV mRNA Testing Correlation
    Dikson Dibe Gondim
    Departments of Pathology and Immunology Radiation Oncology Otolaryngology Head and Neck Surgery, Washington University School of Medicine, St Louis, MO Departments of Pathology, Microbiology, and Immunology Otolaryngology, Vanderbilt University Medical Center, Nashville, TN
    Am J Surg Pathol 40:1117-24. 2016
    ..In summary, strictly defined nonkeratinizing OPSCC (which constitutes ∼55% of all tumors) essentially implies positivity for both p16 and transcriptionally active high-risk HPV. ..
  16. pmc PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park Ave, Saint Louis, MO 63108, USA
    Nucleic Acids Res 40:D1144-9. 2012
    ..The primers and all experimental validation data can be freely accessed from the PrimerBank website, http://pga.mgh.harvard.edu/primerbank/...
  17. pmc Composition of seed sequence is a major determinant of microRNA targeting patterns
    Xiaowei Wang
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63108, USA
    Bioinformatics 30:1377-83. 2014
    ....
  18. pmc Transcriptionally-active human papillomavirus is consistently retained in the distant metastases of primary oropharyngeal carcinomas
    Mitra Mehrad
    Department of Pathology and Immunology, Washington University in St Louis, School of Medicine, Campus Box 8118, 660 S Euclid Ave, St Louis, MO, 63110, USA
    Head Neck Pathol 8:157-63. 2014
    ....
  19. doi request reprint Partial p16 staining in oropharyngeal squamous cell carcinoma: extent and pattern correlate with human papillomavirus RNA status
    James S Lewis
    Department of Pathology and Immunology, Washington University, St Louis, MO 63110, USA
    Mod Pathol 25:1212-20. 2012
    ..These results support that greater than 75% p16 staining or, alternatively, >50% staining combined with >25% confluent areas, are suitable cutoffs for defining positivity...
  20. doi request reprint A miR-200 microRNA cluster as prognostic marker in advanced ovarian cancer
    Xiaoxia Hu
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO 63110, USA
    Gynecol Oncol 114:457-64. 2009
    ..A reliable method that could predict disease outcome would improve care of patients with this disease. The main aim of this study is to identify novel prognostic biomarkers for advanced ovarian cancer...
  21. doi request reprint High E6 Gene Expression Predicts for Distant Metastasis and Poor Survival in Patients With HPV-Positive Oropharyngeal Squamous Cell Carcinoma
    Shariq S Khwaja
    Department of Radiation Oncology, Washington University School of Medicine, St Louis, Missouri
    Int J Radiat Oncol Biol Phys 95:1132-41. 2016
    ..However, approximately 10% of patients will experience distant recurrence even with standard-of-care treatment. Here, we sought to identify novel biomarkers to better risk-stratify HPV-positive patients with OPSCC...
  22. pmc WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
    Nathan Wong
    Department of Biomedical Engineering, Washington University, St Louis, MO, 63130, USA
    Genome Biol 16:218. 2015
    ..These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR ( http://crispr.wustl.edu ). ..
  23. pmc Cardiac lipin 1 expression is regulated by the peroxisome proliferator activated receptor γ coactivator 1α/estrogen related receptor axis
    Mayurranjan S Mitra
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Cell Cardiol 51:120-8. 2011
    ..Collectively, these data suggest that lipin 1 is the principal lipin protein in the myocardium and is regulated in response to physiologic and pathologic stimuli that impact cardiac metabolism...
  24. pmc Systematic identification of microRNA functions by combining target prediction and expression profiling
    Xiaowei Wang
    Ambion, Inc, 2130 Woodward Street, Austin, TX 78744, USA
    Nucleic Acids Res 34:1646-52. 2006
    ..Our high-throughput approach can be generalized to globally identify the targets and functions of other miRNAs...
  25. ncbi request reprint Selection of oligonucleotide probes for protein coding sequences
    Xiaowei Wang
    Department of Molecular Biology, 50 Blossom Street, Massachusetts General Hospital, Boston 02114, USA
    Bioinformatics 19:796-802. 2003
    ..We describe the constraints in oligo design that are imposed by this strategy, as well as a software tool that allows the strategy to be applied broadly...
  26. doi request reprint Salinicoccus halodurans sp. nov., a moderate halophile from saline soil in China
    Xiaowei Wang
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, PR China
    Int J Syst Evol Microbiol 58:1537-41. 2008
    ..nov. is proposed. The type strain is W24(T) (=CGMCC 1.6501(T)=DSM 19336(T))...
  27. ncbi request reprint The let-7 microRNA represses cell proliferation pathways in human cells
    Charles D Johnson
    Asuragen, Inc, Texas, USA
    Cancer Res 67:7713-22. 2007
    ..This work reveals the let-7 microRNA to be a master regulator of cell proliferation pathways...
  28. ncbi request reprint A sequence-oriented comparison of gene expression measurements across different hybridization-based technologies
    Winston Patrick Kuo
    Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Ave, Boston, Massachusetts 02115, USA
    Nat Biotechnol 24:832-40. 2006
    ..We demonstrate that, after stringent preprocessing, commercial arrays were more consistent than in-house arrays, and by most measures, one-dye platforms were more consistent than two-dye platforms...