connexin 45 6

Summary

Gene Symbol: connexin 45 6
Description: gap junction protein, alpha 8, 50kDa
Alias: Cx45.6, Cx50, connexin 45.6, connexin 50, connexin-45.6, gap junction alpha-8 protein
Species: chicken

Top Publications

  1. pmc Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation
    Ke Wang
    State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China
    J Biol Chem 287:15786-97. 2012
  2. pmc Phosphorylation of connexin 50 by protein kinase A enhances gap junction and hemichannel function
    Jialu Liu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229, USA
    J Biol Chem 286:16914-28. 2011
  3. pmc Aquaporin 0 enhances gap junction coupling via its cell adhesion function and interaction with connexin 50
    Jialu Liu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Cell Sci 124:198-206. 2011
  4. pmc Amino acid residue Val362 plays a critical role in maintaining the structure of C terminus of connexin 50 and in lens epithelial-fiber differentiation
    Qian Shi
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    J Biol Chem 285:18415-22. 2010
  5. pmc Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner
    Eric A Banks
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA
    J Cell Sci 122:378-88. 2009
  6. pmc Lens fiber connexin turnover and caspase-3-mediated cleavage are regulated alternately by phosphorylation
    Xinye Yin
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas, USA
    Cell Commun Adhes 15:1-11. 2008
  7. ncbi Promotion of lens epithelial-fiber differentiation by the C-terminus of connexin 45.6 a role independent of gap junction communication
    Eric A Banks
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    J Cell Sci 120:3602-12. 2007
  8. pmc Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels
    Lixian Dong
    Department of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Physiol 576:787-99. 2006
  9. ncbi Developmental regulation of the direct interaction between the intracellular loop of connexin 45.6 and the C terminus of major intrinsic protein (aquaporin-0)
    Xun Sean Yu
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    J Biol Chem 280:22081-90. 2005
  10. ncbi Interaction of major intrinsic protein (aquaporin-0) with fiber connexins in lens development
    X Sean Yu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Cell Sci 117:871-80. 2004

Detail Information

Publications14

  1. pmc Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation
    Ke Wang
    State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China
    J Biol Chem 287:15786-97. 2012
    ..We have shown previously that Cx50 is likely to be cleaved by caspase-3 like protease during chick lens development...
  2. pmc Phosphorylation of connexin 50 by protein kinase A enhances gap junction and hemichannel function
    Jialu Liu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229, USA
    J Biol Chem 286:16914-28. 2011
    ..Here, we showed by mass spectrometry that Ser-395 in the C terminus of chicken Cx50 was phosphorylated in the lens. Ser-395 is located within a PKA consensus site...
  3. pmc Aquaporin 0 enhances gap junction coupling via its cell adhesion function and interaction with connexin 50
    Jialu Liu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Cell Sci 124:198-206. 2011
    Both connexin 50 (Cx50) and aquaporin 0 (AQP0) have important roles in lens development and homeostasis, and their mutations are associated with human congenital cataracts. We have previously shown that Cx50 directly interacts with AQP0...
  4. pmc Amino acid residue Val362 plays a critical role in maintaining the structure of C terminus of connexin 50 and in lens epithelial-fiber differentiation
    Qian Shi
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    J Biol Chem 285:18415-22. 2010
    ..Here, we show that deletion of the PEST motif at the C terminus (CT) domain of Cx50 attenuates the stimulatory effect of Cx50 on lens fiber differentiation...
  5. pmc Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner
    Eric A Banks
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA
    J Cell Sci 122:378-88. 2009
    ..When expressed in paired Xenopus oocytes, human (h) and chicken (ch) Cx50 E48K mutants showed no electrical coupling...
  6. pmc Lens fiber connexin turnover and caspase-3-mediated cleavage are regulated alternately by phosphorylation
    Xinye Yin
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas, USA
    Cell Commun Adhes 15:1-11. 2008
    ..6 by caspase-3. These findings provide further insights into regulatory mechanism of the specific phosphorylation of connexins in the lens...
  7. ncbi Promotion of lens epithelial-fiber differentiation by the C-terminus of connexin 45.6 a role independent of gap junction communication
    Eric A Banks
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    J Cell Sci 120:3602-12. 2007
    ..Here, we took advantage of naturally occurring loss-of-gap-junction function mutations of Cx50 (ortholog of chick Cx45.6) and generated the corresponding site mutants in Cx45.6: Cx45.6(D47A) and Cx45.6(P88S)...
  8. pmc Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels
    Lixian Dong
    Department of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Physiol 576:787-99. 2006
    Previous studies have shown that gap junctional channels formed from the lens connexins Cx50 (or its chicken orthologue, Cx45.6) and Cx43 exhibit marked differences in transjunctional voltage gating and unitary conductance...
  9. ncbi Developmental regulation of the direct interaction between the intracellular loop of connexin 45.6 and the C terminus of major intrinsic protein (aquaporin-0)
    Xun Sean Yu
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    J Biol Chem 280:22081-90. 2005
    ..Finally this interaction had no impact on Cx45.6-mediated intercellular communication, suggesting that the Cx45.6-MIP(AQP0) interaction plays a novel unidentified role in lens fibers...
  10. ncbi Interaction of major intrinsic protein (aquaporin-0) with fiber connexins in lens development
    X Sean Yu
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Cell Sci 117:871-80. 2004
    ....
  11. ncbi The development-associated cleavage of lens connexin 45.6 by caspase-3-like protease is regulated by casein kinase II-mediated phosphorylation
    X Yin
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    J Biol Chem 276:34567-72. 2001
    ..Finally, caspase-3-mediated cleavage can be regulated by casein kinase II-mediated phosphorylation, suggesting that Cx45.6 turnover and specific cleavage by caspase-3-like protease is alternatively modulated...
  12. ncbi Casein kinase II phosphorylates lens connexin 45.6 and is involved in its degradation
    X Yin
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78284 7760, USA
    J Biol Chem 275:6850-6. 2000
    Connexin (Cx) 45.6, an avian counterpart of rodent Cx50, is phosphorylated in vivo, but the sites and function of the phosphorylation have not been elucidated...
  13. ncbi Characterization of terminally differentiated cell state by categorizing cDNA clones derived from chicken lens fibers
    K Sawada
    Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan
    Int J Dev Biol 40:531-5. 1996
    ..The mRNA composition in the chicken lens fiber cells indicated rather simple organization of mRNA species of this cell type, and gave scope to the possibility of full description of differentiated lens fiber cells at gene activity level...
  14. pmc Molecular cloning and functional characterization of chick lens fiber connexin 45.6
    J X Jiang
    Department of Cell Biology, Neurobiology Harvard Medical School, Boston 02115
    Mol Biol Cell 5:363-73. 1994
    ..6 and ChCx56 were abundant in lens fibers. Treatment of lens membranes with alkaline phosphatase resulted in electrophoretic mobility shifts, demonstrating that both ChCx45.6 and ChCx56 were phosphoproteins in vivo...