dam

Summary

Gene Symbol: dam
Description: DNA adenine methyltransferase
Alias: ECK3374, JW3350
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutants
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    DNA Repair (Amst) 4:773-81. 2005
  2. pmc Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli
    Jennifer L Robbins-Manke
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Ave, 56 670, Cambridge, MA 02139, USA
    J Bacteriol 187:7027-37. 2005
  3. pmc N6-methyl-adenine: an epigenetic signal for DNA-protein interactions
    Didier Wion
    INSERM U318, CHU Michallon, Universite Joseph Fourier, 38043 Grenoble, France
    Nat Rev Microbiol 4:183-92. 2006
  4. pmc The eclipse period of Escherichia coli
    U von Freiesleben
    Department of Microbiology, The Technical University of Denmark, Building 301, DK 2800 Lyngby, Denmark
    EMBO J 19:6240-8. 2000
  5. ncbi Eclipse period without sequestration in Escherichia coli
    Jan Olsson
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    Mol Microbiol 44:1429-40. 2002
  6. ncbi Novel growth rate control of dam gene expression in Escherichia coli
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Mol Microbiol 12:631-8. 1994
  7. ncbi Characterization of Escherichia coli mutants with altered ploidy
    N J Trun
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Res Microbiol 142:195-200. 1991
  8. pmc Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR
    A Bergerat
    Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes de Saclay, Gif sur Yvette, France
    Proc Natl Acad Sci U S A 88:6394-7. 1991
  9. ncbi The gene for 2-phosphoglycolate phosphatase (gph) in Escherichia coli is located in the same operon as dam and at least five other diverse genes
    A Lyngstadaas
    Department of Cell Biology, Institute of Cancer Research, Montebello, Oslo, Norway
    Biochim Biophys Acta 1472:376-84. 1999
  10. pmc Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: mechanistic exploration of epigenetic gene regulation
    Adam J Pollak
    Department of Chemistry and Biochemistry University of California, Santa Barbara, California 93106 9510, USA
    J Biol Chem 287:22873-81. 2012

Research Grants

  1. DNA Mismatch and Double-Strand Break Repair
    Martin G Marinus; Fiscal Year: 2010

Scientific Experts

  • Anthony R Poteete
  • I Matic
  • Erik Boye
  • Martin Marinus
  • Didier Wion
  • Daghfous Douraid
  • A Jeltsch
  • A Lyngstadaas
  • Aswin Sai Narain Seshasayee
  • Kirsten Liebert
  • John R Horton
  • Norbert O Reich
  • Stephanie R Coffin
  • Sanjay Chahar
  • Xiaodong Cheng
  • Adam J Pollak
  • Daniela K Jacquelín
  • Jongchul Bang
  • Daniel J Ferullo
  • Stacey N Peterson
  • Svetlana Bulanenkova
  • Douraid Daghfous
  • Pierre Brézellec
  • Anders Løbner-Olesen
  • U von Freiesleben
  • Jennifer L Robbins-Manke
  • Anetta Nowosielska
  • Neda Mashhoon
  • Hanjing Yang
  • L J Rasmussen
  • Baljinder K Sohanpal
  • Jesse D McCool
  • Jennifer Wyrzykowski
  • Melissa A Calmann
  • Jan A Olsson
  • Nina Costantino
  • Andrea Hermann
  • Kurt Nordström
  • Santanu Dasgupta
  • Magdalena Gabig
  • Jason Correnti
  • Jan Olsson
  • Denise E Waldron
  • Sabine Urig
  • A Løbner-Olesen
  • D C Bell
  • T Onogi
  • R Vaisvila
  • W Haagmans
  • Sergey Ragozin
  • M Reddy
  • Hany Elsawy
  • Chin Ju Park
  • Carlos E Argaraña
  • S Kang
  • I R Henderson
  • Sung Hun Bae
  • José L Barra
  • Byong Seok Choi
  • Mariana A Martina
  • Joon Hwa Lee
  • Susan T Lovett
  • Lev Nikolaev
  • Eugene Snezhkov
  • Eugene Sverdlov
  • Marcella Orwick
  • Sophie Pasek
  • Miklos Bekes
  • Jean Luc Ferat
  • Ahmed Landoulsi
  • Marie Sophie Hiet
  • Abdlelwaheb Chatti
  • Brahim Marzouk
  • Mark Hoebeke
  • Zoran Z Zdraveski
  • Stanley Hattman
  • John M Essigmann
  • Ole Skovgaard
  • Maryam Lahooti
  • Martina Schlickenrieder
  • Joerg Eberhard
  • Michael Carroll
  • Sammia El-Labany
  • Amy Diep
  • Norbert Reich
  • Hilary A Sandler
  • Susan M Rosenberg
  • Jacqueline A Plumbridge
  • Ian C Blomfield
  • Jeffrey H Miller

Detail Information

Publications81

  1. ncbi Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutants
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    DNA Repair (Amst) 4:773-81. 2005
    Escherichia coli dam cells are more susceptible to the cytotoxic action of cisplatin than wildtype...
  2. pmc Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli
    Jennifer L Robbins-Manke
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Ave, 56 670, Cambridge, MA 02139, USA
    J Bacteriol 187:7027-37. 2005
    DNA adenine methylation by DNA adenine methyltransferase (Dam) in Escherichia coli plays an important role in processes such as DNA replication initiation, gene expression regulation, and mismatch repair. In addition, E...
  3. pmc N6-methyl-adenine: an epigenetic signal for DNA-protein interactions
    Didier Wion
    INSERM U318, CHU Michallon, Universite Joseph Fourier, 38043 Grenoble, France
    Nat Rev Microbiol 4:183-92. 2006
    ..In gamma-proteobacteria, Dam methylation regulates chromosome replication, nucleoid segregation, DNA repair, transposition of insertion elements ..
  4. pmc The eclipse period of Escherichia coli
    U von Freiesleben
    Department of Microbiology, The Technical University of Denmark, Building 301, DK 2800 Lyngby, Denmark
    EMBO J 19:6240-8. 2000
    ..An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation...
  5. ncbi Eclipse period without sequestration in Escherichia coli
    Jan Olsson
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    Mol Microbiol 44:1429-40. 2002
    ..For strains with seqA, dam and damseqA mutants, the length of the eclipse period was 0.16, 0.40 and 0.32 generation times respectively...
  6. ncbi Novel growth rate control of dam gene expression in Escherichia coli
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Mol Microbiol 12:631-8. 1994
    Transcription of the dam gene in Escherichia coli is growth rate regulated by a mechanism distinct from that used for ribosomal RNA gene promoters...
  7. ncbi Characterization of Escherichia coli mutants with altered ploidy
    N J Trun
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Res Microbiol 142:195-200. 1991
    ..these cell cycle mutants, we have coupled them with defects in recA, to test for induction of the SOS response, and dam, to determine if methylation is required for mbr function...
  8. pmc Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR
    A Bergerat
    Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes de Saclay, Gif sur Yvette, France
    Proc Natl Acad Sci U S A 88:6394-7. 1991
    ..The methylation reaction was also studied in the presence of a competitive inhibitor and this showed two successive stages involved in the methylation and two sites of binding for S-adenosylmethionine...
  9. ncbi The gene for 2-phosphoglycolate phosphatase (gph) in Escherichia coli is located in the same operon as dam and at least five other diverse genes
    A Lyngstadaas
    Department of Cell Biology, Institute of Cancer Research, Montebello, Oslo, Norway
    Biochim Biophys Acta 1472:376-84. 1999
    Downstream of the dam gene in the Escherichia coli genome the following three genes are located: first rpe, then a gene encoding a 27 kDa protein and finally trpS...
  10. pmc Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: mechanistic exploration of epigenetic gene regulation
    Adam J Pollak
    Department of Chemistry and Biochemistry University of California, Santa Barbara, California 93106 9510, USA
    J Biol Chem 287:22873-81. 2012
    The methylation of adenine in palindromic 5'-GATC-3' sites by Escherichia coli Dam supports diverse roles, including the essential regulation of virulence genes in several human pathogens...
  11. pmc Very-short-patch repair in Escherichia coli requires the dam adenine methylase
    D C Bell
    Biology Department, Concordia University, Montreal, Quebec H3G 1M8, Canada
    J Bacteriol 183:3631-5. 2001
    Strains of Escherichia coli which lack the dam-encoded adenine methylase are mutators due to a reduction in the efficiency of postreplication mismatch repair...
  12. ncbi Competitive interaction of the OxyR DNA-binding protein and the Dam methylase at the antigen 43 gene regulatory region in Escherichia coli
    Denise E Waldron
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Republic of Ireland
    Mol Microbiol 44:509-20. 2002
    ..agn43 transcription start site in a region of DNA that encompasses three 5'-GATC-3' sequences that are subject to Dam-mediated DNA methylation...
  13. pmc Limiting DNA replication to once and only once
    E Boye
    Department of Cell Biology, Institute for Cancer Research, Montebello, Oslo, Norway
    EMBO Rep 1:479-83. 2000
    ..All cells have developed mechanisms for origin inactivation, but those of other bacteria and eukaryotic cells are clearly distinct from those of E. coli. Possible differences and similarities are discussed...
  14. ncbi The Escherichia coli dam DNA methyltransferase modifies DNA in a highly processive reaction
    Sabine Urig
    Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Mol Biol 319:1085-96. 2002
    The Escherichia coli dam adenine-N6 methyltransferase modifies DNA at GATC sequences. It is involved in post-replicative mismatch repair, control of DNA replication and gene regulation. We show that E...
  15. pmc The Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions
    Jennifer Wyrzykowski
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 185:1701-4. 2003
    ..In addition, we show that a dam mutant has an increased sensitivity to hydrogen peroxide treatment that can be suppressed by mutations that ..
  16. pmc Regulated expression of the Escherichia coli dam gene
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 185:5012-4. 2003
    Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site...
  17. pmc Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants
    Nina Costantino
    Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Building 539, PO Box B, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 100:15748-53. 2003
    ..Our results show that Beta is the only bacteriophage function required for this level of recombination and suggest that Beta directs the ssDNA to the replication fork as it passes the target sequence...
  18. pmc Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants
    T Onogi
    Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862 0976, Japan
    J Bacteriol 182:5898-901. 2000
    ..We show here that the associated muk null mutations cause hypersensitivity to novobiocin. Null mutation of either dam or seqA suppressed partially the temperature-sensitive lethality but failed to suppress the anucleate cell ..
  19. pmc Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli
    M Reddy
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Bacteriol 182:1978-86. 2000
    ..are divisible into two categories, depending upon whether they did (uup, ssb, polA, and topA) or did not (mutHLS, dam, and uvrD) also increase precise excision frequency of the mini-Tn10 derivatives...
  20. ncbi Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach
    Hanjing Yang
    Department of Microbiology, Immunology and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA
    Mol Microbiol 53:283-95. 2004
    ..known to cause mutator effects under overexpression conditions: error-prone polymerase IV (DinB), DNA methylase (Dam) and sigma S factor (RpoS)...
  21. ncbi Stopped-flow and mutational analysis of base flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase
    Kirsten Liebert
    School of Engineering and Science, International University Bremen, Campus Ring 1, 28759 Bremen, Germany
    J Mol Biol 341:443-54. 2004
    ..using 2-aminopurine as a probe to detect base flipping, we show here that base flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase (MTase) is a biphasic process: target base flipping is very fast (k(flip)>240 ..
  22. ncbi Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12
    M G Marinus
    Mutat Res 28:15-26. 1975
    The dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-meA) residues in the DNA of E. coli K12 or phage lambda. The DNA of phage fd appears to be devoid of 6-meA when propagated on dam-3 bacteria...
  23. ncbi Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E. coli Dam methylase
    Svetlana Bulanenkova
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
    Genetica 130:83-92. 2007
    Using transient expression of the E. coli Dam methylase gene and analysis of the distribution of methylated GATC sites, we studied the distribution of open chromatin regions within a 140 kb long human genome segment in HEK-293 cells...
  24. doi Changing the DNA recognition specificity of the EcoDam DNA-(adenine-N6)-methyltransferase by directed evolution
    Sanjay Chahar
    School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28725 Bremen, Germany
    J Mol Biol 395:79-88. 2010
    ..We conclude that the new methyltransferases are fully functional in vivo and in vitro but show a new target-site specificity...
  25. pmc Escherichia coli DNA adenine methyltransferase: the structural basis of processive catalysis and indirect read-out
    Stephanie R Coffin
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106 9510, USA
    J Biol Chem 284:18390-400. 2009
    ..This study describes the structural means by which an enzyme that does not completely enclose its substrate has evolved to achieve processive catalysis, and how interactions with DNA flanking the recognition site alter this processivity...
  26. pmc Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA
    BMC Mol Biol 10:14. 2009
    ..To explore the mechanism of expansion, a 7 kbp duplication in the chromosome containing a leaky mutant version of the lac operon was constructed, and its expansion into an amplified array was studied...
  27. doi Structural and dynamics study of DNA dodecamer duplexes that contain un-, hemi-, or fully methylated GATC sites
    Jongchul Bang
    Department of Chemistry and National Creative Research Initiative Center, KAIST, 373 1, Guseong Dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    J Am Chem Soc 130:17688-96. 2008
    ..The two G.C base pairs of the hemimethylated GATC duplex displayed a faster base-pair opening rate and required less energy for the base-pair opening reaction than did those of the fully methylated one...
  28. pmc The stringent response and cell cycle arrest in Escherichia coli
    Daniel J Ferullo
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA
    PLoS Genet 4:e1000300. 2008
    ..Arrest of replication and inhibition of colony formation by ppGpp accumulation is relieved in seqA and dam mutants, although other aspects of the stringent response appear to be intact...
  29. pmc Modulation of Escherichia coli DNA methyltransferase activity by biologically derived GATC-flanking sequences
    Stephanie R Coffin
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106 9510, USA
    J Biol Chem 283:20106-16. 2008
    ....
  30. ncbi Plasmid expression of mutS, -L and/or -H gene in Escherichia coli dam cells results in strains that display reduced mutation frequency
    Daniela K Jacquelín
    Centro de Investigaciones en Quimica Biologica de Cordoba, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, X5000HUA, Cordoba, Argentina
    Mutat Res 637:197-204. 2008
    ..Unpaired double-strand breaks on a fraction of the replication errors occurring in dam cells presumably cause cell death, selectively eliminating these putative mutants from the population...
  31. pmc An assessment of the role of DNA adenine methyltransferase on gene expression regulation in E coli
    Aswin Sai Narain Seshasayee
    Genomics and Regulatory Systems Group, EMBL European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    PLoS ONE 2:e273. 2007
    ..In gamma-proteobacteria, Dam is a stand-alone enzyme that methylates GATC sites, which are non-randomly distributed in the genome...
  32. ncbi Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy
    Jesse D McCool
    Department of Microbiology, Morrill Science Center IV N203, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Mol Microbiol 53:1343-57. 2004
    ..The patterns displayed by priA, dnaT, recG, uvrD, dam, ftsK, rnhA, polA and xerC mutants were explained best by the TPM while only lexA (def), lexA3 (ind-) and recA ..
  33. ncbi DomainSieve: a protein domain-based screen that led to the identification of dam-associated genes with potential link to DNA maintenance
    Pierre Brézellec
    Laboratoire Statistique et Génome du CNRS, Tour Evry 2, 523, Place des Terrasses de l Agora, 91034 Evry Cedex, France
    Bioinformatics 22:1935-41. 2006
    The Dam methyltransferase (DamMT) activity, broadly distributed in association with restriction endonucleases, as part of the restriction-modification defense systems, has evolved to become intimately associated with essential biological ..
  34. ncbi Phospholipid changes in seqA and dam mutants of Escherichia coli
    Douraid Daghfous
    Laboratoire de Biochimie et de Biologie Moleculaire, Faculte des Sciences de Bizerte, 7021 Zarzouna, Tunisie
    C R Biol 329:271-6. 2006
    SeqA and Dam proteins were known to be responsible for regulating the initiation of replication and to affect the expression of many genes and metabolisms...
  35. pmc Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Mol Biol 358:559-70. 2006
    The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine...
  36. pmc Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA
    Ivan Matic
    INSERM U571, Faculte de Medecine, Universite Paris V, 156 rue Vaugirard, 75730 Paris Cedex 15, France
    J Bacteriol 188:339-42. 2006
    ..initiated by the incorporation of the base analog 2-aminopurine kills DNA-methylation-deficient Escherichia coli dam cells by DNA double-strand breakage...
  37. pmc Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 121:349-61. 2005
    ..These structures illustrate the transition in enzyme-DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts...
  38. pmc Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12
    Baljinder K Sohanpal
    Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    Proc Natl Acad Sci U S A 101:16322-7. 2004
    ..The NanR- and NagC-binding sites lie adjacent to deoxyadenosine methylase (Dam) methylation sites (5'-GATC) that are protected from modification, and the two regulators are shown to be required ..
  39. ncbi Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics
    Neda Mashhoon
    EpiGenX Pharmaceuticals, Pacific Technology Center, Santa Barbara, California 93111, USA
    J Biol Chem 279:52075-81. 2004
    ..Our studies provide a basis for further structural and functional analysis of this important enzyme and for the identification of inhibitors for potential therapeutic applications...
  40. ncbi Characterization of three genes in the dam-containing operon of Escherichia coli
    A Lyngstadaas
    Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway
    Mol Gen Genet 247:546-54. 1995
    The dam-containing operon in Escherichia coli is located at 74 min on the chromosomal map and contains the genes aroK, aroB, a gene called urf74.3, dam and trpS...
  41. pmc The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli
    A Bergerat
    Departement de Biologie, Centre d Etudes Nucleaires de Saclay, Gif sur Yvette, France
    Nucleic Acids Res 18:4369-75. 1990
    ..coli strongly decreases when the temperature is lowered. This has allowed us to study the binding of Dam methylase on 14 bp DNA fragments at 0 degrees C by gel retardation in the presence of Ado-Met, but without ..
  42. ncbi The Escherichia coli dam gene is expressed as a distal gene of a new operon
    P Jonczyk
    Department of Biology, University of California, San Diego, La Jolla 92093
    Mol Gen Genet 217:85-96. 1989
    DNA containing the Escherichia coli dam gene and sequences upstream from this gene were cloned from the Clarke-Carbon plasmids pLC29-47 and pLC13-42...
  43. ncbi The effect of dam methylation on the expression of glnS in E. coli
    J Plumbridge
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511
    Biochimie 69:539-41. 1987
    The wild type glnS promoter contains a dam methylation site. In dam strains, the expression of glnS is enhanced 2.6-fold. A mutated form of the promoter has been isolated in which the dam methylation site is lost...
  44. pmc GATC sequence and mismatch repair in Escherichia coli
    F Laengle-Rouault
    EMBO J 5:2009-13. 1986
    ..The presence of GATC sequence(s) in the substrate appears to be required for full mismatch repair activity and not only for its strand specificity according to the GATC methylation state...
  45. pmc Cloning and characterization of the gene for Escherichia coli tryptophanyl-transfer ribonucleic acid synthetase
    C V Hall
    J Bacteriol 148:941-9. 1981
    ..We also describe the construction of an inphase trpS-lacZ gene fusion that is under the control of the trpS promoter and encodes a hybrid protein with beta-galactosidase activity...
  46. pmc The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene
    J E Brooks
    Nucleic Acids Res 11:837-51. 1983
    The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1...
  47. ncbi Phase variation of Ag43 in Escherichia coli: Dam-dependent methylation abrogates OxyR binding and OxyR-mediated repression of transcription
    W Haagmans
    Department of Microbiology, University of Pennsylvania, 202A Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Mol Microbiol 35:877-87. 2000
    ..protein Antigen43 (Ag43) of Escherichia coli requires the DNA-methylating enzyme deoxyadenosine methyltransferase (Dam) and the global regulator OxyR...
  48. ncbi Expression of the Escherichia coli dam gene
    A Løbner-Olesen
    Department of Microbiology, Technical University of Denmark, Lyngby, Copenhagen
    Mol Microbiol 6:1841-51. 1992
    The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3...
  49. ncbi Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12
    B R McGraw
    Mol Gen Genet 178:309-15. 1980
    Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered...
  50. ncbi Growth-rate-dependent transcription initiation from the dam P2 promoter
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655, USA
    Gene 157:213-5. 1995
    Transcription of the dam gene in Escherichia coli is dependent on growth rate...
  51. pmc Quantitation of Dam methyltransferase in Escherichia coli
    E Boye
    Department of Biophysics, Institute for Cancer Research, Oslo, Norway
    J Bacteriol 174:1682-5. 1992
    An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E...
  52. ncbi Identification of a weak promoter for the dam gene of Escherichia coli
    T H Wu
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Biochim Biophys Acta 1131:47-52. 1992
    ..identify a weak promoter located about 70 nucleotides before the start site of translation of the Escherichia coli dam gene which encodes a DNA methyltransferase...
  53. ncbi On the substrate specificity of DNA methyltransferases. adenine-N6 DNA methyltransferases also modify cytosine residues at position N4
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Biol Chem 274:19538-44. 1999
    ..We show here that the M.EcoRV, M.EcoRI, and Escherichia coli dam methyltransferases as well as the N- and C-terminal domains of the M...
  54. pmc Repair of heteroduplex DNA molecules with multibase loops in Escherichia coli
    M Carraway
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 175:3972-80. 1993
    ..The repair of both the mismatch and the loops was directed by the state of dam methylation of the DNA chains and was dependent on the product of the mutS gene...
  55. pmc The role of the preserved sequences of Dam methylase
    J B Guyot
    Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes de Saclay, Gif sur Yvette, France
    Nucleic Acids Res 21:3183-90. 1993
    We have undertaken a site directed mutational analysis of two of the preserved regions in the amino acid sequence of Dam methylase in order to characterize their role...
  56. ncbi Both an altered DNA structure and cellular proteins are involved in protecting a triplex forming an oligopurine-rich sequence from Dam methylation in E. coli
    J Klysik
    Institute of Biosciences and Technology, Texas A and M University, Texas Medical Center, Houston 77030, USA
    Biochem Genet 34:165-78. 1996
    When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA...
  57. pmc Overexpression of the wild-type gene coding for Escherichia coli DNA adenine methylase (dam)
    V U Nwosu
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Biochem J 283:745-50. 1992
    The gene coding for Escherichia coli dam methylase was isolated from a dam+ K12 strain by the PCR method. The gene was subcloned into an overexpression vector under the control of the strong lambda PL promoter...
  58. pmc Chromosome partitioning in Escherichia coli in the absence of dam-directed methylation
    D Vinella
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universite Paris, France
    J Bacteriol 174:2388-90. 1992
    Escherichia coli dam mutants, lacking the GATC DNA methylase, do not produce anucleate cells at high frequencies, suggesting that hemimethylation of the chromosome origin of replication, oriC, is not essential for correct chromosome ..
  59. pmc Gene transcription and chromosome replication in Escherichia coli
    P Zhou
    Department of Biological Sciences, Florida Institute of Technology, Melbourne 32901, USA
    J Bacteriol 179:163-9. 1997
    ..Levels of transcripts from four of the genes, dam, nrdA, mukB, and seqA, were reduced at a certain stage during chromosome replication...
  60. pmc The major phase-variable outer membrane protein of Escherichia coli structurally resembles the immunoglobulin A1 protease class of exported protein and is regulated by a novel mechanism involving Dam and oxyR
    I R Henderson
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland
    J Bacteriol 181:2132-41. 1999
    ..Evidence strongly suggests that phase variation is regulated by both deoxyadenosine methylase (Dam) and by OxyR...
  61. ncbi Interaction of SeqA and Dam methylase on the hemimethylated origin of Escherichia coli chromosomal DNA replication
    S Kang
    Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 274:11463-8. 1999
    ..SeqA protein to hemimethylated oriC, the origin of Escherichia coli chromosomal replication, delays methylation by Dam methylase...
  62. ncbi The cell surface protein Ag43 facilitates phage infection of Escherichia coli in the presence of bile salts and carbohydrates
    Magdalena Gabig
    Department of Molecular Biology, University of Gdansk, Kładki 24, 80 822 Gdansk, Poland
    Microbiology 148:1533-42. 2002
    ....
  63. ncbi Eclipse-synchrony relationship in Escherichia coli strains with mutations affecting sequestration, initiation of replication and superhelicity of the bacterial chromosome
    Jan A Olsson
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    J Mol Biol 334:919-31. 2003
    ..is also believed to be responsible for initiation synchrony, since inactivation of either the seqA or the dam gene abolishes synchrony while drastically reducing the eclipse...
  64. doi Competitive Lrp and Dam assembly at the pap regulatory region: implications for mechanisms of epigenetic regulation
    Stacey N Peterson
    Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106, USA
    J Mol Biol 383:92-105. 2008
    Escherichia coli DNA adenine methyltransferase (Dam) and Leucine-responsive regulatory protein (Lrp) are key regulators of the pap operon, which codes for the pilus proteins necessary for uropathogenic E. coli cellular adhesion...
  65. ncbi High-level expression of a semisynthetic dam gene in Escherichia coli
    K H Hülsmann
    Institut fur Kristallographie, Freie Universitat Berlin, F R G
    Gene 98:83-8. 1991
    We constructed a semisynthetic gene encoding a DNA-adenine-methyltransferase (Dam) that codes for the same amino acid sequence as the wild type (wt) Escherichia coli dam gene...
  66. ncbi Dam methylation: coordinating cellular processes
    Anders Løbner-Olesen
    Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    Curr Opin Microbiol 8:154-60. 2005
    ..DamMT has also been implicated as a virulence factor in bacterial pathogenesis. The origin and phylogeny of DamMT, based on sequenced genomes, has been deduced...
  67. ncbi Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle
    Kirsten Liebert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, 28759 Bremen, Germany
    J Biol Chem 282:22848-55. 2007
    ..This suggests that the hexapeptide couples specific DNA binding (Lys(9)), AdoMet binding (Trp(10)), and insertion of the flipped target base into the active site pocket (Lys(14))...
  68. ncbi dam methylation and the initiation of DNA replication on oriC plasmids
    A Landoulsi
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universite Paris VII, France
    Mol Gen Genet 216:217-23. 1989
    ..coli chromosome (oriC) has been shown to be inefficient as a template for DNA synthesis in vitro when isolated from dam mutants...
  69. pmc Sequence and substrate specificity of isolated DNA methylases from Escherichia coli C
    S Urieli-Shoval
    J Bacteriol 153:274-80. 1983
    ..of Escherichia coli C, the mec (designates DNA-cytosine-methylase gene, which is also designated dcm) and dam gene products, were physically separated by DEAE-cellulose column chromatography...
  70. pmc Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene
    J A Arraj
    J Bacteriol 153:562-5. 1983
    A recombinant plasmid, pMQ3, carrying the dam gene of Escherichia coli K-12, was constructed and transformed into dam+ and dam- strains...
  71. ncbi The dam and dcm strains of Escherichia coli--a review
    B R Palmer
    Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand
    Gene 143:1-12. 1994
    ..of strains deficient in the methylation of adenine and cytosine residues in DNA by the methyltransferases (MTases) Dam and Dcm has allowed the study of the role of these enzymes in the biology of Escherichia coli...
  72. pmc Analysis of the genetic requirements for viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants
    K R Peterson
    Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721
    J Bacteriol 175:7505-8. 1993
    RecBCD protein, necessary for Escherichia coli dam mutant viability, is directly required for DNA repair...
  73. ncbi Dam-dependent phase variation of Ag43 in Escherichia coli is altered in a seqA mutant
    Jason Correnti
    202A Johnson Pavilion, Department of Microbiology, School of Medicine, University of Pennsylvania, 3610 Hamilton Walk, Philadelphia 19104 6076, USA
    Mol Microbiol 44:521-32. 2002
    ..Transcription of agn43 occurs (ON phase) when three Dam target sequences in the agn43 regulatory region are methylated, which prevents the repressor OxyR from binding...
  74. ncbi The LipB protein is a negative regulator of dam gene expression in Escherichia coli
    R Vaisvila
    New England Biolabs, Beverly MA 01915 5599, USA
    Biochim Biophys Acta 1494:43-53. 2000
    Transcription initiation of the major promoter (P2) of the Escherichia coli dam gene increases with growth rate...
  75. pmc Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutant
    M G Marinus
    Department of Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 182:463-8. 2000
    Double mutants of Escherichia coli dam (DNA adenine methyltransferase) strains with ruvA, ruvB, or ruvC could not be constructed, whereas dam derivatives with recD, recF, recJ, and recR were viable...

Research Grants1

  1. DNA Mismatch and Double-Strand Break Repair
    Martin G Marinus; Fiscal Year: 2010
    ..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..