dnaB

Summary

Gene Symbol: dnaB
Description: replicative DNA helicase
Alias: ECK4044, JW4012, groP, grpA, grpD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Bound Lac repressor protein differentially inhibits the unwinding reactions catalyzed by DNA helicases
    J E Yancey-Wrona
    Department of Biology, University of North Carolina, Chapel Hill 27599
    Nucleic Acids Res 20:6713-21. 1992
  2. ncbi The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties
    E Wahle
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 264:2463-8. 1989
  3. ncbi The Escherichia coli dnaB replication protein is a DNA helicase
    J H Lebowitz
    J Biol Chem 261:4738-48. 1986
  4. ncbi Structure and function of Escherichia coli DnaB protein: role of the N-terminal domain in helicase activity
    S B Biswas
    Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201, USA
    Biochemistry 33:11307-14. 1994
  5. pmc Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA
    Y B Lu
    Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:12902-7. 1996
  6. ncbi Mechanism of DnaB helicase of Escherichia coli: structural domains involved in ATP hydrolysis, DNA binding, and oligomerization
    E E Biswas
    Department of Molecular Biology, SOM, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA
    Biochemistry 38:10919-28. 1999
  7. ncbi pH-controlled quaternary states of hexameric DnaB helicase
    L E Donate
    Campus de Cantoblanco, Centro Nacional de Biotecnologia CSIC, Madrid, 28049, Spain
    J Mol Biol 303:383-93. 2000
  8. ncbi Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 278:52253-61. 2003
  9. pmc Helicobacter pylori DnaB helicase can bypass Escherichia coli DnaC function in vivo
    Rajesh K Soni
    Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India
    Biochem J 389:541-8. 2005
  10. pmc Directed evolution of ionizing radiation resistance in Escherichia coli
    Dennis R Harris
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53575, USA
    J Bacteriol 191:5240-52. 2009

Research Grants

  1. MECHANISM OF DNA-PROTEIN INTERACTION IN DNA REPLICATION
    Subhasis Biswas; Fiscal Year: 2002
  2. MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS
    Deepak Bastia; Fiscal Year: 2008
  3. MECHANISM OF TERMINATION OF DNA REPLICATION
    Deepak Bastia; Fiscal Year: 2010
  4. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
  5. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010

Scientific Experts

  • W M BUJALOWSKI
  • SUSAN LOVETT
  • Hideo Ikeda
  • P Soultanas
  • Deepak Bastia
  • Mark A Griep
  • Daniel L Kaplan
  • Subhasis Biswas
  • Eiko Akaboshi
  • Cameron Neylon
  • Anasuya Roychowdhury
  • Maria J Jezewska
  • Roberto Galletto
  • Jan Gert Brüning
  • M J Jezewska
  • Ernesto Arias-Palomo
  • Kathryn L Molt
  • Tara Kashav
  • Dennis R Harris
  • Michal R Szymanski
  • E E Biswas
  • Nathan A Tanner
  • Nicholas E Dixon
  • Christian J Rudolph
  • Suman Kumar Dhar
  • Ryan C Heller
  • Stephen J Watt
  • Yoshito Abe
  • Xilin Zhao
  • Nathalie Michel-Reydellet
  • Rajesh K Soni
  • Roderick A Slavcev
  • Julian A Tanner
  • O K Kaboev
  • N E Dixon
  • Barbara Kedzierska
  • Peter McGlynn
  • Stephen Flowers
  • Atanaska V Mitkova
  • Kamila Katarzyna Myka
  • Joseph A DeVito
  • Christoph Weigel
  • Iris V Hood
  • James M Berger
  • Valerie L O'Shea
  • K Hanada
  • K J Marians
  • S K Johnson
  • H G Dallmann
  • H Seitz
  • J Marszalek
  • L E Donate
  • Elizabeth A Wood
  • Sarit A Klugman
  • Hao Li
  • Wolfram Saenger
  • Michael C Popelars
  • Eric L Cabot
  • Lukas B Bane
  • Thomas J Albert
  • Steve V Pollock
  • John R Battista
  • Jason E Norton
  • Wendy Schackwitz
  • Reece J Goiffon
  • Vincent A Sutera
  • Michael M Cox
  • Azat Gabdoulkhakov
  • Julie Eggington
  • Joel Martin
  • Len A Pennacchio
  • Audrey J Klingele
  • TIMOTHY J DURFEE
  • Ramgopal Nitharwal
  • Nicole T Perna
  • Lindsay L Hamilton
  • Kathryn K Moore
  • S E Brown
  • Samudrala Gourinath
  • S Arif Abdulrehman
  • Christina M Middle
  • Samir M Hamdan
  • Antoine M van Oijen
  • L Fang
  • Karin V Loscha
  • Slobodan Jergic
  • J M Jones
  • Patrick M Schaeffer
  • J M Carazo
  • J M Kaguni

Detail Information

Publications108 found, 100 shown here

  1. pmc Bound Lac repressor protein differentially inhibits the unwinding reactions catalyzed by DNA helicases
    J E Yancey-Wrona
    Department of Biology, University of North Carolina, Chapel Hill 27599
    Nucleic Acids Res 20:6713-21. 1992
    ..The helicase reactions catalyzed by E. coli DnaB protein and helicase IV were substantially inhibited by the presence of bound protein...
  2. ncbi The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties
    E Wahle
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 264:2463-8. 1989
    The complex formed between the dnaB and dnaC replication proteins of Escherichia coli is stabilized by ATP binding to dnaC. The dnaB6-dnaC6-ATP6 complex can be maintained without ATP hydrolysis at a concentration as low as 5 x 10(-10) M...
  3. ncbi The Escherichia coli dnaB replication protein is a DNA helicase
    J H Lebowitz
    J Biol Chem 261:4738-48. 1986
    Genetic and biochemical analyses indicate that the Escherichia coli dnaB replication protein functions in the propagation of replication forks in the bacterial chromosome...
  4. ncbi Structure and function of Escherichia coli DnaB protein: role of the N-terminal domain in helicase activity
    S B Biswas
    Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201, USA
    Biochemistry 33:11307-14. 1994
    We have analyzed the contributions of specific domains of DnaB helicase to its quaternary structure and multienzyme activities. Highly purified tryptic fragments containing various domains of DnaB helicase were prepared...
  5. pmc Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA
    Y B Lu
    Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:12902-7. 1996
    The primase DnaG of Escherichia coli requires the participation of the replicative helicase DnaB for optimal synthesis of primer RNA for lagging strand replication...
  6. ncbi Mechanism of DnaB helicase of Escherichia coli: structural domains involved in ATP hydrolysis, DNA binding, and oligomerization
    E E Biswas
    Department of Molecular Biology, SOM, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA
    Biochemistry 38:10919-28. 1999
    We describe the delineation of three distinct structural domains of the DnaB helicase of Escherichia coli: domain alpha, amino acid residues (aa) 1-156; domain beta, aa 157-302; and domain gamma, aa 303-471...
  7. ncbi pH-controlled quaternary states of hexameric DnaB helicase
    L E Donate
    Campus de Cantoblanco, Centro Nacional de Biotecnologia CSIC, Madrid, 28049, Spain
    J Mol Biol 303:383-93. 2000
    b>DnaB is the major helicase in the Escherichia coli replisome. It is a homohexameric enzyme that interacts with many other replisomal proteins and cofactors...
  8. ncbi Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 278:52253-61. 2003
    ..synthesis of RNA primers in the lagging strand of the replication fork in Escherichia coli requires the replicative DnaB helicase and the DNA primase, the DnaG gene product...
  9. pmc Helicobacter pylori DnaB helicase can bypass Escherichia coli DnaC function in vivo
    Rajesh K Soni
    Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India
    Biochem J 389:541-8. 2005
    In Escherichia coli, DnaC is essential for loading DnaB helicase at oriC (the origin of chromosomal DNA replication)...
  10. pmc Directed evolution of ionizing radiation resistance in Escherichia coli
    Dennis R Harris
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53575, USA
    J Bacteriol 191:5240-52. 2009
    ..At least some evolutionary pathways to extreme radiation resistance are constrained by the temporally ordered appearance of specific alleles...
  11. pmc Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro
    S Wickner
    Proc Natl Acad Sci U S A 72:921-5. 1975
    Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro...
  12. ncbi Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12
    H Saito
    J Mol Biol 113:1-25. 1977
  13. ncbi Escherichia coli Hfr-DNA degradation in endonuclease I-deficient minicells
    G G Khachatourians
    Biochim Biophys Acta 561:294-300. 1979
    ..These experiments suggest that the presence of the endI- mutation in minicell-producing strain chi1268 leads to a greater loss in M2 of Hfr DNA conjugally transferred into the minicells...
  14. pmc Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12
    K Hasunuma
    J Bacteriol 137:1095-9. 1979
    Escherichia coli strains with mutations in genes dnaB, dnaC, and dnaG were tested for their capacity to replicate pSC101 deoxyribonucleic acid (DNA) at a nonpermissive temperature...
  15. pmc Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids
    S E Conrad
    Nucleic Acids Res 6:3289-304. 1979
    ..In addition, we find that proteins encoded by dnaB, dnaC, dnaG, dnaI, dnaP and polC (DNA polymerase III), are required for in vitro plasmid synthesis...
  16. pmc Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli
    M A McIntosh
    J Bacteriol 122:592-8. 1975
    ....
  17. ncbi Fine balance in the regulation of DnaB helicase by DnaC protein in replication in Escherichia coli
    G C Allen
    Department of Biochemistry, Stanford University School of Medicine, California 94305
    J Biol Chem 266:22096-101. 1991
    ..In the initiation of replication of a minichromosome at its origin, DnaC delivers the DnaB helicase from a DnaB.DnaC complex to the future replication fork and then departs...
  18. pmc Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction
    M Dodson
    Proc Natl Acad Sci U S A 83:7638-42. 1986
    ..Second, lambda P and host DnaB proteins interact with the O-some to generate a larger complex that includes additional DNA from an A + T-rich ..
  19. ncbi The specificity of Escherichia coli endonuclease I
    S D Ehrlich
    Eur J Biochem 40:149-53. 1973
  20. ncbi Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyl/transferase
    V A Lightner
    J Biol Chem 255:9413-20. 1980
    ..in a second locus involved in phospholipid biosynthesis, dgk (diglyceride kinase), and one of them also bears the dnaB structural gene. Diglyceride kinase activity is also overproduced in these strains...
  21. ncbi Cloning of the dnaB gene of Escherichia coli: the dnaB gene of groPB534 and groPB612 and the replication of phage lambda
    E Gunther
    Mol Gen Genet 193:225-30. 1984
    ..The resulting recombinant plasmids contained the genes uvrA, groP (B534 or B612), and lexA...
  22. ncbi Structural and functional studies of the dnaB protein using limited proteolysis. Characterization of domains for DNA-dependent ATP hydrolysis and for protein association in the primosome
    N Nakayama
    J Biol Chem 259:88-96. 1984
    Two separable structural domains were identified in the Escherichia coli dnaB protein (Mr = 52,000) by partial proteolytic cleavage under nondenaturing conditions...
  23. ncbi Interactions of Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA. The nucleic acid does not wrap around the protein hexamer
    W Bujalowski
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 1053, USA
    Biochemistry 34:8513-9. 1995
    The interactions of the Escherichia coli primary replicative helicase DnaB protein with single-stranded (ss) DNA have been studied using the thermodynamically rigorous fluorescence titration technique, which allowed us to obtain absolute ..
  24. pmc Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein
    W Bujalowski
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555 0653
    J Biol Chem 269:31350-8. 1994
    The oligomeric structure of the Escherichia coli primary replicative helicase DnaB protein in relation to the functions of the enzyme and the energetics of its stability has been characterized...
  25. ncbi Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli
    A Skokotas
    Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104
    J Biol Chem 269:20446-55. 1994
    ..4100-fold lower affinity for Ter sites than wild type Tus and was unable to halt DNA replication in vivo or inhibit DnaB-catalyzed strand displacement in an in vitro helicase assay...
  26. ncbi Replication origin of the broad host range plasmid RK2. Positioning of various motifs is critical for initiation of replication
    K S Doran
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 273:8447-53. 1998
    ..altered origins were analyzed for replication activity in vivo and in vitro, for localized strand opening and for DnaB helicase mediated unwinding...
  27. ncbi Functional and structural heterogeneity of the DNA binding site of the Escherichia coli primary replicative helicase DnaB protein
    M J Jezewska
    Department of Human Biological Chemistry and Genetics and the Sealy Center for Structural Biology, University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1053, USA
    J Biol Chem 273:9058-69. 1998
    The structure-function relationship within the DNA binding site of the Escherichia coli replicative helicase DnaB protein was studied using nuclease digestion, quantitative fluorescence titration, centrifugation, and fluorescence energy ..
  28. ncbi Duplex opening by primosome protein PriA for replisome assembly on a recombination intermediate
    J M Jones
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, 3900 Reservoir Rd NW, Washington, DC, 20007, USA
    J Mol Biol 289:503-16. 1999
    PriA and other primosome assembly proteins of Escherichia coli recruit the major replicative helicase DnaB for replisome assembly during bacteriophage Mu transposition and replication...
  29. ncbi NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer
    J Weigelt
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Structure 7:681-90. 1999
    b>DnaB is the primary replicative helicase in Escherichia coli. Native DnaB is a hexamer of identical subunits, each consisting of a larger C-terminal domain and a smaller N-terminal domain...
  30. ncbi Initiation of bidirectional replication at the chromosomal origin is directed by the interaction between helicase and primase
    H Hiasa
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 274:27244-8. 1999
    ....
  31. pmc Role of DnaB helicase in UV-induced illegitimate recombination in Escherichia coli
    K Hanada
    The Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    J Bacteriol 183:4964-9. 2001
    ..of DNA replication in UV-induced illegitimate recombination, we examined the effect of temperature-sensitive dnaB mutations on illegitimate recombination and found that the frequency of illegitimate recombination was reduced by ..
  32. ncbi An array of target-specific screening strains for antibacterial discovery
    Joseph A DeVito
    Department of Antimicrobial Research, Bristol Myers Squibb Company, Wilmington, DE 19880, USA
    Nat Biotechnol 20:478-83. 2002
    ..As an example of the target specificity of the approach, compounds identified in the whole-cell screen for MurA inhibitors were also found to block the biochemical function of the target when tested in vitro...
  33. ncbi Conformational dynamics of DnaB helicase upon DNA and nucleotide binding: analysis by intrinsic tryptophan fluorescence quenching
    Stephen Flowers
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 42:1910-21. 2003
    b>DnaB helicase of E. coli unwinds duplex DNA in the replication fork using the energy of ATP hydrolysis...
  34. ncbi Expanding the physiological role of the hexameric DnaB helicase
    Wlodzimierz Bujalowski
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, 77555 1053, USA
    Trends Biochem Sci 28:116-8. 2003
    The hexameric DnaB protein is the Escherichia coli primary replicative helicase...
  35. ncbi Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase: the effect of the 3' arm and the stability of the dsDNA on the unwinding activity of the Escherichia coli DnaB helicase
    Roberto Galletto
    Department of Human Biological Chemistry and Genetics, the Sealy Center for Structural Biology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, 77555 1053, USA
    J Mol Biol 343:101-14. 2004
    ..double-stranded DNA (dsDNA) part, on the kinetics of the dsDNA unwinding by the Escherichia coli hexameric helicase DnaB protein has been examined under single turnover conditions using the rapid quench-flow technique...
  36. pmc Replication fork stalling and cell cycle arrest in UV-irradiated Escherichia coli
    Christian J Rudolph
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
    Genes Dev 21:668-81. 2007
    ..All DNA synthesis after UV depends on DnaC, implying that replication restart of blocked forks requires DnaB loading and possibly the entire assembly of new replisomes...
  37. ncbi Non-replicative helicases at the replication fork
    Ryan C Heller
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    DNA Repair (Amst) 6:945-52. 2007
    ..Restart systems operate to restore the 5'-->3' replicative helicase, DnaB, to the lagging-strand template...
  38. pmc A role for nonessential domain II of initiator protein, DnaA, in replication control
    Kathryn L Molt
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 183:39-49. 2009
    ..DnaA binds to multiple sequences at the origin of replication, oriC, unwinding the DNA and promoting the binding of DnaB helicase...
  39. pmc Interactions of the Escherichia coli DnaB-DnaC protein complex with nucleotide cofactors. 1. Allosteric conformational transitions of the complex
    Anasuya Roychowdhury
    Department of Biochemistry and Molecular Biology, The Sealy Center for Structural Biology and Molecular Biophysics, Sealy Center for Cancer Cell Biology, The University of Texas Medical Branch at Galveston, Texas 77555 1053, USA
    Biochemistry 48:6712-29. 2009
    Interactions of nucleotide cofactors with both protein components of the Escherichia coli DnaB helicase complex with the replication factor, the DnaC protein, have been examined using MANT-nucleotide analogues...
  40. pmc The bacterial DnaC helicase loader is a DnaB ring breaker
    Ernesto Arias-Palomo
    Department of Molecular and Cell Biology, California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 153:438-48. 2013
    ..coli DnaB⋅DnaC helicase/loader complex...
  41. pmc Overexpression of the Replicative Helicase in Escherichia coli Inhibits Replication Initiation and Replication Fork Reloading
    Jan Gert Brüning
    Department of Biology, University of York, Wentworth Way, York YO10 5DD, United Kingdom
    J Mol Biol 428:1068-79. 2016
    ..The Escherichia coli replicative helicase DnaB and the helicase loader DnaC form a DnaB6-DnaC6 complex that is required for loading DnaB onto single-stranded DNA...
  42. ncbi The release of endonuclease I from Escherichia coli by a new cold shock procedure
    H N Ananthaswamy
    Biochem Biophys Res Commun 76:289-98. 1976
  43. pmc Release of periplasmic enzymes from Escherichia coli by penicillin-ethylenediaminetetraacetate treatment
    H N Ananthaswamy
    J Bacteriol 131:710-2. 1977
    ..However, incubation of the penicillin-induced spheroplasts in a medium containing ethylenediaminetetraacetic acid caused the release of endonuclease I and cyclic phosphodiesterase but not of beta-galactosidase...
  44. ncbi The dnaB gene product of Escherichia coli. I. Purification, homogeneity, and physical properties
    L J Reha-Krantz
    J Biol Chem 253:4043-50. 1978
    The dnaB gene product was purified to homogeneity and its physical properties were characterized. Purification was aided by the use of the Escherichia coli strain. MV12/28, which overproduced the dnaB gene product 10-fold (Wickner, S. H...
  45. ncbi The Escherichia coli dnaB protein is required for initiation of chromosomal DNA replication
    F K Fujimura
    Cold Spring Harb Symp Quant Biol 43:559-62. 1979
  46. ncbi Escherichia coli DNA helicases: mechanisms of DNA unwinding
    T M Lohman
    Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110 1093
    Mol Microbiol 6:5-14. 1992
    ..coli Rep, RecBCD and phage T7 gene 4 helicases...
  47. ncbi Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. I. Multiple effectors act to modulate Okazaki fragment size
    C A Wu
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 267:4030-44. 1992
    ..Here we demonstrate that some enzymes, such as the DnaB helicase, remain associated continuously with the fork while others, such as the primase, must be recruited from ..
  48. ncbi Physical interactions between bacteriophage and Escherichia coli proteins required for initiation of lambda DNA replication
    K Liberek
    Department of Molecular Biology, University of Gdansk, Poland
    J Biol Chem 265:3022-9. 1990
    ..The lambda P initiator protein binds to both lambda O and the host-coded dnaB helicase, giving rise to an ori lambda DNA.lambda O.lambda P.dnaB structure...
  49. ncbi The dnaB-dnaC replication protein complex of Escherichia coli. II. Role of the complex in mobilizing dnaB functions
    E Wahle
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 264:2469-75. 1989
    The dnaC protein of Escherichia coli, by forming a complex with the dnaB protein, facilitates the interactions with single-stranded DNA that enable dnaB to perform its ATPase, helicase, and priming functions...
  50. pmc Escherichia coli replication termination protein impedes the action of helicases
    E H Lee
    Department of Biochemistry, Stanford University, CA 94305 5307
    Proc Natl Acad Sci U S A 86:9104-8. 1989
    ..The target for blockage within the replication complex can now be ascribed to the inability of dnaB helicase to separate the duplex strands when it encounters ter bound by TBP...
  51. ncbi The dnaB protein of Escherichia coli: mechanism of nucleotide binding, hydrolysis, and modulation by dnaC protein
    E E Biswas
    Biochemistry 25:7368-74. 1986
    The mechanism of nucleotide binding and hydrolysis by dnaB protein and dnaB X dnaC protein complex has been studied by using fluorescent nucleotide analogues...
  52. ncbi Revised sequence of the nusA gene of Escherichia coli and identification of nusA11 (ts) and nusA1 mutations which cause changes in a hydrophobic amino acid cluster
    M Saito
    Mol Gen Genet 205:380-2. 1986
    ..In the course of these experiments, several mistakes in the published nusA nucleotide sequence were found. These errors are revised in this article. The molecular weight of NusA is accordingly revised to 54,430...
  53. ncbi Helicase action of dnaB protein during replication from the Escherichia coli chromosomal origin in vitro
    T A Baker
    J Biol Chem 262:6877-85. 1987
    ..From a complex containing proteins dnaA, dnaB, and dnaC bound at oriC, the dnaB helicase moves in both directions to unwind the duplex...
  54. ncbi Isolation and characterization of a mutant of Escherichia coli K12 synthesizing DNA polymerase I and endonuclease I constitutively
    S I Ahmad
    J Gen Microbiol 117:419-22. 1980
    ..The mutant strain apparently repairs its damaged DNA more efficiently than wild-type E. coli K12 strains and, to do so, constitutively produces 35 times more DNA polymerase I and 12 times more endonuclease I than the wild-type strain...
  55. ncbi Mechanism of dnaB protein action. I. Crystallization and properties of dnaB protein, an essential replication protein in Escherichia coli
    K Arai
    J Biol Chem 256:5247-52. 1981
    Purification and crystallization of dnaB protein from Escherichia coli was performed on a large scale by a simple procedure. From 1.5 kg of cells, 520 mg of dnaB protein were obtained in a 58% yield with a purity greater than 99%. The E...
  56. ncbi Host components required for the replication of the resistance plasmid R124 and a copy mutant derivative
    J J Pritchard
    Z Allg Mikrobiol 20:129-40. 1980
    ..replication of R124, and a copy mutant derivative of it, was measured with respect to dependence on the host DnaA, DnaB, DnaC, DnaE, DnaG, and PolA gene products...
  57. ncbi Involvement of host cell gene products in conversion of bacteriophage S13 single-stranded DNa to duplex replicative form DNA in vitro
    K Watabe
    J Biochem 90:779-83. 1981
    ..Using extracts from some thermosensitive mutants of E. coli defective in DNA replication, we found that dnaB and dnaC gene products are involved in the conversion stage of single-stranded DNA to duplex DNA in vitro.
  58. pmc High frequency of genetic duplications in the dnaB region of the Escherichia coli K12 chromosome
    R A Sclafani
    Genetics 98:677-89. 1981
    The region that includes the dnaB locus on the E. coli K12 chromosome was shown to be duplicated at high frequency in cell populations. The duplications were shown to be arranged in tandem and segregated at various frequencies...
  59. ncbi Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner
    S Kleinsteuber
    Institut fur Genetik, Martin Luther Universitat Halle Wittenberg, Halle Saale, Germany
    Mol Gen Genet 248:695-702. 1995
    The replicative DNA helicase encoded by the dnaB gene is essential for chromosomal DNA replication in Escherichia coli...
  60. pmc Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454
    D Saluja
    Department of Biochemistry, New York University Medical Center, New York 10016
    J Bacteriol 177:1104-11. 1995
    By use of PCR, the dnaB genes from the classical temperature-sensitive dnaB mutants PC8 (dnaB8), RS162 (dnaB252), CR34/454 (dnaB454), HfrH165/70 (dnaB70), and CR34/43 (dnaB43) were isolated...
  61. ncbi Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
    P P Cherepanov
    Universitat Oldenburg, Germany
    Gene 158:9-14. 1995
    ..This system was applied in the construction of an E. coli endA deletion mutation which can be transduced by P1 to the genetic background of interest using TcR as a marker. The transductant can then be freed of the TcR if required...
  62. ncbi Analysis of the endA mutation of Escherichia coli K12 strains: JM103 behaves like endA+ wild-type strains
    S Wnendt
    Gruenenthal GmbH, Aachen, FRG
    Biotechniques 17:270, 272. 1994
  63. ncbi The speed of the Escherichia coli fork in vivo depends on the DnaB:DnaC ratio
    K Skarstad
    Department of Biophysics, Institute for Cancer Research, Oslo, Norway
    Mol Microbiol 17:825-31. 1995
    The DnaC protein is required for loading the DnaB helicase at oriC. Thus DnaC promotes the formation of the pre-replication complex, but must leave the complex in order for the DnaB protein to function as a helicase...
  64. ncbi The hexameric E. coli DnaB helicase can exist in different Quaternary states
    X Yu
    Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis 55455, USA
    J Mol Biol 259:7-14. 1996
    The DnaB protein is the primary replicative helicase in Escherichia coli, and the active form of the protein is a hexamer...
  65. ncbi Binding of Escherichia coli primary replicative helicase DnaB protein to single-stranded DNA. Long-range allosteric conformational changes within the protein hexamer
    M J Jezewska
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 1053, USA
    Biochemistry 35:2129-45. 1996
    Quantitative analyses of the interactions of the Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA have been performed using the thermodynamically rigorous fluorescence titration technique...
  66. pmc DNA double-strand breaks caused by replication arrest
    B Michel
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    EMBO J 16:430-8. 1997
    ..Two E. coli replicative helicases were tested, Rep, which is essential in recBC mutants, and DnaB. The proportion of linear DNA increased up to 50% upon shift of rep recBTS recCTS cells to restrictive temperature...
  67. pmc Cryptic single-stranded-DNA binding activities of the phage lambda P and Escherichia coli DnaC replication initiation proteins facilitate the transfer of E. coli DnaB helicase onto DNA
    B A Learn
    Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:1154-9. 1997
    The bacteriophage lambda P and Escherichia coli DnaC proteins are known to recruit the bacterial DnaB replicative helicase to initiator complexes assembled at the phage and bacterial origins, respectively...
  68. ncbi Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC
    C San Martin
    Centro Nacional de Biotecnologia, Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    Structure 6:501-9. 1998
    ..b>DnaB is the major replicative DNA helicase in Escherichia coli and has been used as a model system for studying the ..
  69. ncbi Escherichia coli DnaA protein. The N-terminal domain and loading of DnaB helicase at the E. coli chromosomal origin
    M D Sutton
    Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824 1319, USA
    J Biol Chem 273:34255-62. 1998
    ..A step that follows is the binding of DnaB helicase at oriC so that it is properly positioned at each replication fork...
  70. ncbi Recognition of sequence-directed structure of the ssDNA backbone by nucleases
    E Akaboshi
    Institute for Molecular and Cellular Biology, Osaka University, 1 3 Yamadaoka, Suita 565, Japan
    Biochem Biophys Res Commun 256:532-6. 1999
    ..The digestion patterns were examined in relation to previous results of biochemical and crystallographic studies on dsDNA, and showed broad agreement. Endonuclease I cleaved ssDNA at sites corresponding to bent sites in dsDNA...
  71. ncbi Mechanism of DNA binding by the DnaB helicase of Escherichia coli: analysis of the roles of domain gamma in DNA binding
    E E Biswas
    Department of Molecular Biology, SOM, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA
    Biochemistry 38:10929-39. 1999
    We have analyzed the mechanism of single-stranded DNA (ssDNA) binding mediated by the C-terminal domain gamma of the DnaB helicase of Escherichia coli...
  72. ncbi Replisome assembly at oriC, the replication origin of E. coli, reveals an explanation for initiation sites outside an origin
    L Fang
    Microbiology Department, Joan and Sanford I Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Mol Cell 4:541-53. 1999
    ..coli origin, oriC. We show that two hexamers of DnaB assemble onto the opposing strands of the resulting bubble, expanding it further, yet helicase action is not ..
  73. ncbi DnaB helicase stimulates primer synthesis activity on short oligonucleotide templates
    S K Johnson
    Department of Chemistry, Center for Biotechnology, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0304, USA
    Biochemistry 39:736-44. 2000
    b>DnaB helicase stimulated the second-order RNA primer synthesis activity of primase by over 5000-fold on DNA templates that were 23 nucleotides long...
  74. ncbi Characterization of the unique C terminus of the Escherichia coli tau DnaX protein. Monomeric C-tau binds alpha AND DnaB and can partially replace tau in reconstituted replication forks
    H G Dallmann
    Department of Biochemistry and Molecular Genetics and Molecular Biology Program, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 275:15512-9. 2000
    A contact between the dimeric tau subunit within the DNA polymerase III holoenzyme and the DnaB helicase is required for replication fork propagation at physiologically-relevant rates (Kim, S., Dallmann, H. G., McHenry, C. S...
  75. ncbi The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli
    H Seitz
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, D 14195 Berlin Dahlem, Germany
    Mol Microbiol 37:1270-9. 2000
    The initiation of chromosome replication in Escherichia coli requires the recruitment of the replicative helicase DnaB from the DnaBC complex to the unwound region within the replication origin oriC, supported by the oriC-bound initiator ..
  76. ncbi DnaB drives DNA branch migration and dislodges proteins while encircling two DNA strands
    Daniel L Kaplan
    Laboratory of DNA Replication, Rockefeller University, New York, NY 10021, USA
    Mol Cell 10:647-57. 2002
    b>DnaB is a ring-shaped, hexameric helicase that unwinds the E. coli DNA replication fork while encircling one DNA strand. This report demonstrates that DnaB can also encircle both DNA strands and then actively translocate along the duplex...
  77. ncbi Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC
    Christoph Weigel
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 46:1149-56. 2002
    We analysed the enzymatic activity (strand dis-placement) of the Escherichia coli DnaB helicase on a mirror-image pair of oligonucleotide-based substrates mimicking the unwound replication origin oriC...
  78. ncbi Toxicity of the bacteriophage lambda cII gene product to Escherichia coli arises from inhibition of host cell DNA replication
    Barbara Kedzierska
    Department of Molecular Biology, University of Gdansk, Kladki 24, 80 822 Gdansk, Poland
    Virology 313:622-8. 2003
    ..coli dnaB and dnaC genes...
  79. ncbi Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase: quantitative analysis of the rate of the dsDNA unwinding, processivity and kinetic step-size of the Escherichia coli DnaB helicase using rapid quench-flow method
    Roberto Galletto
    Department of Human Biological Chemistry and Genetics, the Sealy Center for Structural Biology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, 77555 1053, USA
    J Mol Biol 343:83-99. 2004
    Kinetics of the double-stranded (ds) DNA unwinding by the Escherichia coli replicative helicase DnaB protein has been examined under single-turnover conditions using the chemical quench-flow technique...
  80. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..that RecQ plays a role in the suppression of RecEmediated illegitimate recombination, with the participation of DnaB, Fis, Exol, and H-NS...
  81. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..that RecQ plays a role in the suppression of RecE-mediated illegitimate recombination, with the participation of DnaB, Fis, ExoI, and H-NS...
  82. pmc Identification and characterization of a novel allele of Escherichia coli dnaB helicase that compromises the stability of plasmid P1
    Roderick A Slavcev
    Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8
    J Bacteriol 187:1227-37. 2005
    ..coli chromosomal mutations that influenced P1 stability and identified a novel temperature-sensitive allele of the dnaB helicase gene (dnaB277) that replaces serine 277 with a leucine residue (DnaB S277L)...
  83. ncbi The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus
    Julian A Tanner
    Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong, China
    Chem Biol 12:303-11. 2005
    ..Kinetics of inhibition are consistent with bananin inhibiting an intracellular process or processes involved in SCV replication...
  84. pmc Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex
    Cameron Neylon
    School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom
    Microbiol Mol Biol Rev 69:501-26. 2005
    ..The Tus-Ter complex acts by blocking the action of the replicative DnaB helicase, but details of the mechanism are uncertain...
  85. ncbi Increasing PCR fragment stability and protein yields in a cell-free system with genetically modified Escherichia coli extracts
    Nathalie Michel-Reydellet
    Department of Chemical Engineering, Stanford University, Stanford, Calif 94305 5025, USA
    J Mol Microbiol Biotechnol 9:26-34. 2005
    ..With this system, it was possible to rapidly express a range of cytoplasmic and periplasmic proteins...
  86. pmc Lethal action of quinolones against a temperature-sensitive dnaB replication mutant of Escherichia coli
    Xilin Zhao
    Public Health Research Institute, 225 Warren St, Newark, New Jersey 07103, USA
    Antimicrob Agents Chemother 50:362-4. 2006
    Inhibition of DNA replication in an Escherichia coli dnaB-22 mutant failed to block quinolone-mediated lethality...
  87. ncbi Structure and function of DnaA N-terminal domains: specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC
    Yoshito Abe
    Department of Immunology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 282:17816-27. 2007
    ..The interaction of the DnaA N terminus with the DnaB helicase is crucial for the loading of DnaB onto the unwound region...
  88. ncbi Myricetin inhibits Escherichia coli DnaB helicase but not primase
    Mark A Griep
    Department of Chemistry, University of Nebraska Lincoln, 614 Hamilton Hall, Lincoln, NE 68588 0304, USA
    Bioorg Med Chem 15:7203-8. 2007
    Primase and DnaB helicase play central roles during DNA replication initiation and elongation...
  89. pmc Single-molecule studies of fork dynamics in Escherichia coli DNA replication
    Nathan A Tanner
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:170-6. 2008
    ..When coupled to the replicative helicase DnaB, Pol III mediates leading-strand synthesis with a processivity of 10...
  90. pmc Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase
    Deepak Bastia
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29528, USA
    Proc Natl Acad Sci U S A 105:12831-6. 2008
    ..arrest at sequence-specific replication termini (Ter) by antagonizing DNA unwinding by the replicative helicase DnaB. Here, we report that Tus is also a polar antitranslocase...
  91. doi Mechanisms of polar arrest of a replication fork
    Daniel L Kaplan
    Vanderbilt University Department of Biological Sciences, VU Station B, Nashville, TN 372352, USA
    Mol Microbiol 72:279-85. 2009
    ..In yeast, terminator sequences are present in rDNA non-transcribed spacers and a region immediately preceding the mating type switch locus Mat1, and the mechanism of polar arrest at these regions is beginning to be elucidated...
  92. pmc Mechanism of NTP hydrolysis by the Escherichia coli primary replicative helicase DnaB protein. 2. Nucleotide and nucleic acid specificities
    Anasuya Roychowdhury
    Department of Biochemistry and Molecular Biology, The Sealy Center for Structural Biology and Molecular Biophysics, Sealy Center for Cancer Cell Biology, The University of Texas Medical Branch at Galveston, Texas 77555 1053, USA
    Biochemistry 48:6730-46. 2009
    The kinetic mechanism of NTP binding and hydrolysis by the Escherichia coli replicative helicase, the DnaB protein, in the absence and presence of the single-stranded DNA (ssDNA), has been quantitatively examined using the rapid quench-..
  93. pmc Three-dimensional structure of N-terminal domain of DnaB helicase and helicase-primase interactions in Helicobacter pylori
    Tara Kashav
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    PLoS ONE 4:e7515. 2009
    Replication initiation is a crucial step in genome duplication and homohexameric DnaB helicase plays a central role in the replication initiation process by unwinding the duplex DNA and interacting with several other proteins during the ..
  94. ncbi Regulation of dnaB function in DNA replication in Escherichia coli by dnaC and lambda P gene products
    S B Biswas
    J Biol Chem 262:7831-8. 1987
    The dnaB protein of Escherichia coli, a multifunctional DNA-dependent ribonucleotide triphosphatase and dATPase, cross-links to ATP on ultraviolet irradiation under conditions that support rNTPase and dATPase activities of dnaB protein...
  95. ncbi Complete enzymatic replication of plasmids containing the origin of the Escherichia coli chromosome
    B E Funnell
    J Biol Chem 261:5616-24. 1986
    ..the origin of the Escherichia coli chromosome, oriC, formation of an active initiation complex consisting of dnaA, dnaB, dnaC, and HU proteins, requires a supercoiled DNA template...
  96. ncbi Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli
    N Nakayama
    J Biol Chem 259:97-101. 1984
    We have determined the nucleotide sequence of the dnaB gene and the primary structure of the dnaB protein of Escherichia coli (Arai, K., Yasuda, S., and Kornberg, A. (1981) J. Biol. Chem. 256, 5247-5252)...
  97. ncbi DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli
    J Marszalek
    Department of Biochemistry, Michigan State University, East Lansing 48824 1319
    J Biol Chem 269:4883-90. 1994
    ..On the analysis of this process, the antibody appears to interfere with the physical interaction between DnaA and DnaB protein in the DnaB.DnaC complex. Cross-linking studies confirm that DnaA and DnaB proteins interact directly...
  98. ncbi Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli DnaB Helicase? Fluorescence energy transfer studies
    M J Jezewska
    Department of Human Biological Chemistry and Genetics and the Sealy Center for Structural Biology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1053, USA
    J Biol Chem 273:10515-29. 1998
    The structure of the complex of the Escherichia coli primary replicative helicase DnaB protein with single-stranded (ss) DNA and replication fork substrates has been examined using the fluorescence energy transfer method...
  99. ncbi Effect of protein stabilization on charge state distribution in positive- and negative-ion electrospray ionization mass spectra
    Stephen J Watt
    Chemistry Department, University of Wollongong, New South Wales, Australia
    J Am Soc Mass Spectrom 18:1605-11. 2007
    ..The N-terminal domain of the Escherichia coli DnaB protein was cyclized by in vivo formation of an amide bond between its N- and C-termini...

Research Grants5

  1. MECHANISM OF DNA-PROTEIN INTERACTION IN DNA REPLICATION
    Subhasis Biswas; Fiscal Year: 2002
    ..A new DNA helicase which is uniquely homologous to DnaB helicase and SV40 T antigen helicase has been identified...
  2. MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS
    Deepak Bastia; Fiscal Year: 2008
    ..Our long term goal is to extend crystallography further by co-crystallizing ternary complexes of peptides of DnaB, DnaA, DnaG that interact with p -iteron DNA complex with the objective of further extending our knowledge of ..
  3. MECHANISM OF TERMINATION OF DNA REPLICATION
    Deepak Bastia; Fiscal Year: 2010
    ..Finally, we wish to uncover the mode of action of the Swi1 and Swi3 proteins to determine if these act as "antisweepases". ..
  4. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
    ..We will extend genetic analysis of radC to determine what role it may play in repair and recombination. ..
  5. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010
    ..LAY DESCRIPTION: We will study a protein present in all bacteria that appears to control their growth. This will provide important missing information about how bacteria divide and may provide a target for antibiotics. ..