dnaB

Summary

Gene Symbol: dnaB
Description: replicative DNA helicase
Alias: ECK4044, JW4012, groP, grpA, grpD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Harris D, Pollock S, Wood E, Goiffon R, Klingele A, Cabot E, et al. Directed evolution of ionizing radiation resistance in Escherichia coli. J Bacteriol. 2009;191:5240-52 pubmed publisher
    ..At least some evolutionary pathways to extreme radiation resistance are constrained by the temporally ordered appearance of specific alleles. ..
  2. Lu Y, Ratnakar P, Mohanty B, Bastia D. Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA. Proc Natl Acad Sci U S A. 1996;93:12902-7 pubmed
    The primase DnaG of Escherichia coli requires the participation of the replicative helicase DnaB for optimal synthesis of primer RNA for lagging strand replication...
  3. Donate L, Llorca O, Barcena M, Brown S, Dixon N, Carazo J. pH-controlled quaternary states of hexameric DnaB helicase. J Mol Biol. 2000;303:383-93 pubmed
    b>DnaB is the major helicase in the Escherichia coli replisome. It is a homohexameric enzyme that interacts with many other replisomal proteins and cofactors...
  4. Mitkova A, Khopde S, Biswas S. Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis. J Biol Chem. 2003;278:52253-61 pubmed
    ..synthesis of RNA primers in the lagging strand of the replication fork in Escherichia coli requires the replicative DnaB helicase and the DNA primase, the DnaG gene product...
  5. Wahle E, Lasken R, Kornberg A. The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties. J Biol Chem. 1989;264:2463-8 pubmed
    The complex formed between the dnaB and dnaC replication proteins of Escherichia coli is stabilized by ATP binding to dnaC. The dnaB6-dnaC6-ATP6 complex can be maintained without ATP hydrolysis at a concentration as low as 5 x 10(-10) M...
  6. Wickner S, Hurwitz J. Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro. Proc Natl Acad Sci U S A. 1975;72:921-5 pubmed
    Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro...
  7. Soni R, Mehra P, Mukhopadhyay G, Dhar S. Helicobacter pylori DnaB helicase can bypass Escherichia coli DnaC function in vivo. Biochem J. 2005;389:541-8 pubmed publisher
    In Escherichia coli, DnaC is essential for loading DnaB helicase at oriC (the origin of chromosomal DNA replication)...
  8. Dombroski A, Platt T. Structure of rho factor: an RNA-binding domain and a separate region with strong similarity to proven ATP-binding domains. Proc Natl Acad Sci U S A. 1988;85:2538-42 pubmed
    ..The implications of discrete domains for RNA and nucleotide binding are discussed in the context of requirements for specific interactions between RNA-binding and ATP-hydrolysis sites during transcription termination. ..
  9. Kleinsteuber S, Quinones A. Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner. Mol Gen Genet. 1995;248:695-702 pubmed
    The replicative DNA helicase encoded by the dnaB gene is essential for chromosomal DNA replication in Escherichia coli...
  10. Abe Y, Jo T, Matsuda Y, Matsunaga C, Katayama T, Ueda T. Structure and function of DnaA N-terminal domains: specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC. J Biol Chem. 2007;282:17816-27 pubmed
    ..The interaction of the DnaA N terminus with the DnaB helicase is crucial for the loading of DnaB onto the unwound region...

Detail Information

Publications62

  1. Harris D, Pollock S, Wood E, Goiffon R, Klingele A, Cabot E, et al. Directed evolution of ionizing radiation resistance in Escherichia coli. J Bacteriol. 2009;191:5240-52 pubmed publisher
    ..At least some evolutionary pathways to extreme radiation resistance are constrained by the temporally ordered appearance of specific alleles. ..
  2. Lu Y, Ratnakar P, Mohanty B, Bastia D. Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA. Proc Natl Acad Sci U S A. 1996;93:12902-7 pubmed
    The primase DnaG of Escherichia coli requires the participation of the replicative helicase DnaB for optimal synthesis of primer RNA for lagging strand replication...
  3. Donate L, Llorca O, Barcena M, Brown S, Dixon N, Carazo J. pH-controlled quaternary states of hexameric DnaB helicase. J Mol Biol. 2000;303:383-93 pubmed
    b>DnaB is the major helicase in the Escherichia coli replisome. It is a homohexameric enzyme that interacts with many other replisomal proteins and cofactors...
  4. Mitkova A, Khopde S, Biswas S. Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis. J Biol Chem. 2003;278:52253-61 pubmed
    ..synthesis of RNA primers in the lagging strand of the replication fork in Escherichia coli requires the replicative DnaB helicase and the DNA primase, the DnaG gene product...
  5. Wahle E, Lasken R, Kornberg A. The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties. J Biol Chem. 1989;264:2463-8 pubmed
    The complex formed between the dnaB and dnaC replication proteins of Escherichia coli is stabilized by ATP binding to dnaC. The dnaB6-dnaC6-ATP6 complex can be maintained without ATP hydrolysis at a concentration as low as 5 x 10(-10) M...
  6. Wickner S, Hurwitz J. Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro. Proc Natl Acad Sci U S A. 1975;72:921-5 pubmed
    Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro...
  7. Soni R, Mehra P, Mukhopadhyay G, Dhar S. Helicobacter pylori DnaB helicase can bypass Escherichia coli DnaC function in vivo. Biochem J. 2005;389:541-8 pubmed publisher
    In Escherichia coli, DnaC is essential for loading DnaB helicase at oriC (the origin of chromosomal DNA replication)...
  8. Dombroski A, Platt T. Structure of rho factor: an RNA-binding domain and a separate region with strong similarity to proven ATP-binding domains. Proc Natl Acad Sci U S A. 1988;85:2538-42 pubmed
    ..The implications of discrete domains for RNA and nucleotide binding are discussed in the context of requirements for specific interactions between RNA-binding and ATP-hydrolysis sites during transcription termination. ..
  9. Kleinsteuber S, Quinones A. Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner. Mol Gen Genet. 1995;248:695-702 pubmed
    The replicative DNA helicase encoded by the dnaB gene is essential for chromosomal DNA replication in Escherichia coli...
  10. Abe Y, Jo T, Matsuda Y, Matsunaga C, Katayama T, Ueda T. Structure and function of DnaA N-terminal domains: specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC. J Biol Chem. 2007;282:17816-27 pubmed
    ..The interaction of the DnaA N terminus with the DnaB helicase is crucial for the loading of DnaB onto the unwound region...
  11. Georgopoulos C. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc Natl Acad Sci U S A. 1971;68:2977-81 pubmed
    ..This suggests that the groN mutation produces a structural change in the bacterial RNA polymerase such that it can no longer interact properly with the phage N product. ..
  12. Dodson M, Echols H, Wickner S, Alfano C, Mensa Wilmot K, Gomes B, et al. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction. Proc Natl Acad Sci U S A. 1986;83:7638-42 pubmed
    ..Second, lambda P and host DnaB proteins interact with the O-some to generate a larger complex that includes additional DNA from an A + T-rich ..
  13. Carr K, Kaguni J. Escherichia coli DnaA protein loads a single DnaB helicase at a DnaA box hairpin. J Biol Chem. 2002;277:39815-22 pubmed
    ..replication fork movement from the Escherichia coli replication origin (oriC) is the replicative helicase, DnaB. At oriC, two and only two helicase molecules are loaded, one for each replication fork...
  14. Biswas S, Biswas Fiss E. Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex. Biochemistry. 2006;45:11505-13 pubmed
    b>DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli...
  15. Bujalowski W, Klonowska M, Jezewska M. Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. J Biol Chem. 1994;269:31350-8 pubmed
    The oligomeric structure of the Escherichia coli primary replicative helicase DnaB protein in relation to the functions of the enzyme and the energetics of its stability has been characterized...
  16. Bujalowski W, Jezewska M. Kinetic mechanism of nucleotide cofactor binding to Escherichia coli replicative helicase DnaB protein. stopped-flow kinetic studies using fluorescent, ribose-, and base-modified nucleotide analogues. Biochemistry. 2000;39:2106-22 pubmed
    The kinetic mechanism of binding nucleotide cofactors to the Escherichia coli primary replicative helicase DnaB protein has been studied, using the fluorescence stopped-flow technique...
  17. Sutton M, Carr K, Vicente M, Kaguni J. Escherichia coli DnaA protein. The N-terminal domain and loading of DnaB helicase at the E. coli chromosomal origin. J Biol Chem. 1998;273:34255-62 pubmed
    ..A step that follows is the binding of DnaB helicase at oriC so that it is properly positioned at each replication fork...
  18. San Martin C, Radermacher M, Wolpensinger B, Engel A, Miles C, Dixon N, et al. Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC. Structure. 1998;6:501-9 pubmed
    ..b>DnaB is the major replicative DNA helicase in Escherichia coli and has been used as a model system for studying the ..
  19. Zhao X, Malik M, Chan N, Drlica Wagner A, Wang J, Li X, et al. Lethal action of quinolones against a temperature-sensitive dnaB replication mutant of Escherichia coli. Antimicrob Agents Chemother. 2006;50:362-4 pubmed
    Inhibition of DNA replication in an Escherichia coli dnaB-22 mutant failed to block quinolone-mediated lethality...
  20. Seitz H, Weigel C, Messer W. The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli. Mol Microbiol. 2000;37:1270-9 pubmed
    The initiation of chromosome replication in Escherichia coli requires the recruitment of the replicative helicase DnaB from the DnaBC complex to the unwound region within the replication origin oriC, supported by the oriC-bound initiator ..
  21. Miller C, Cohen S. Separate roles of Escherichia coli replication proteins in synthesis and partitioning of pSC101 plasmid DNA. J Bacteriol. 1999;181:7552-7 pubmed
    ..replication proteins DnaA, which is required to initiate replication of both the chromosome and plasmid pSC101, and DnaB, the helicase that unwinds strands during DNA replication, have effects on plasmid partitioning that are distinct ..
  22. Fang L, Davey M, O Donnell M. Replisome assembly at oriC, the replication origin of E. coli, reveals an explanation for initiation sites outside an origin. Mol Cell. 1999;4:541-53 pubmed
    ..coli origin, oriC. We show that two hexamers of DnaB assemble onto the opposing strands of the resulting bubble, expanding it further, yet helicase action is not ..
  23. Hiasa H, Marians K. Initiation of bidirectional replication at the chromosomal origin is directed by the interaction between helicase and primase. J Biol Chem. 1999;274:27244-8 pubmed
    ....
  24. Jezewska M, Rajendran S, Bujalowski W. Functional and structural heterogeneity of the DNA binding site of the Escherichia coli primary replicative helicase DnaB protein. J Biol Chem. 1998;273:9058-69 pubmed
    The structure-function relationship within the DNA binding site of the Escherichia coli replicative helicase DnaB protein was studied using nuclease digestion, quantitative fluorescence titration, centrifugation, and fluorescence energy ..
  25. Paetz W, Nass G. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12. Eur J Biochem. 1973;35:331-7 pubmed
  26. Arai K, Kornberg A. Mechanism of dnaB protein action. II. ATP hydrolysis by dnaB protein dependent on single- or double-stranded DNA. J Biol Chem. 1981;256:5253-9 pubmed
  27. Cherepanov P, Wackernagel W. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene. 1995;158:9-14 pubmed
    ..This system was applied in the construction of an E. coli endA deletion mutation which can be transduced by P1 to the genetic background of interest using TcR as a marker. The transductant can then be freed of the TcR if required. ..
  28. Carr K, Kaguni J. Stoichiometry of DnaA and DnaB protein in initiation at the Escherichia coli chromosomal origin. J Biol Chem. 2001;276:44919-25 pubmed
    ..The prepriming complex then forms, involving the binding of DnaB helicase at oriC so that it is properly positioned at each replication fork...
  29. Heller R, Marians K. Non-replicative helicases at the replication fork. DNA Repair (Amst). 2007;6:945-52 pubmed
    ..Restart systems operate to restore the 5'-->3' replicative helicase, DnaB, to the lagging-strand template...
  30. Yang S, Yu X, VanLoock M, Jezewska M, Bujalowski W, Egelman E. Flexibility of the rings: structural asymmetry in the DnaB hexameric helicase. J Mol Biol. 2002;321:839-49 pubmed
    b>DnaB is the primary replicative helicase in Escherichia coli and the hexameric DnaB ring has previously been shown to exist in two states in the presence of nucleotides...
  31. Chintakayala K, Larson M, Grainger W, Scott D, Griep M, Hinrichs S, et al. Domain swapping reveals that the C- and N-terminal domains of DnaG and DnaB, respectively, are functional homologues. Mol Microbiol. 2007;63:1629-39 pubmed
    The bacterial primase (DnaG)-helicase (DnaB) interaction is mediated by the C-terminal domain of DnaG (p16) and a linker that joins the N- and C-terminal domains (p17 and p33 respectively) of DnaB...
  32. Scherzinger E, Haring V, Lurz R, Otto S. Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteins. Nucleic Acids Res. 1991;19:1203-11 pubmed
    ..The bacterial RNA polymerase, the initiation protein DnaA, and the primosomal proteins DnaB, DnaC, DnaG and DnaT are not required...
  33. Seigneur M, Ehrlich S, Michel B. RuvABC-dependent double-strand breaks in dnaBts mutants require recA. Mol Microbiol. 2000;38:565-74 pubmed
    ..for the accessory replicative helicase Rep or deficient for the main Escherichia coli replicative helicase DnaB. We show that, in the rep mutant, RuvABC-mediated breakage occurs in the absence of the homologous recombination ..
  34. Yu X, Jezewska M, Bujalowski W, Egelman E. The hexameric E. coli DnaB helicase can exist in different Quaternary states. J Mol Biol. 1996;259:7-14 pubmed
    The DnaB protein is the primary replicative helicase in Escherichia coli, and the active form of the protein is a hexamer...
  35. Galletto R, Jezewska M, Bujalowski W. Interactions of the Escherichia coli DnaB helicase hexamer with the replication factor the DnaC protein. Effect of nucleotide cofactors and the ssDNA on protein-protein interactions and the topology of the complex. J Mol Biol. 2003;329:441-65 pubmed
    Quantitative studies of interactions between the Escherichia coli replication factor DnaC protein and the DnaB helicase have been performed using sedimentation velocity and fluorescence energy transfer techniques...
  36. Wechsler J, Nüsslein V, Otto B, Klein A, Bonhoeffer F, Herrmann R, et al. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication. J Bacteriol. 1973;113:1381-8 pubmed
    ..This classification is congruent with that obtained by genetic mapping by using cotransduction frequencies with selected markers in P1 transduction analysis. ..
  37. Nakayama N, Arai N, Kaziro Y, Arai K. Structural and functional studies of the dnaB protein using limited proteolysis. Characterization of domains for DNA-dependent ATP hydrolysis and for protein association in the primosome. J Biol Chem. 1984;259:88-96 pubmed
    Two separable structural domains were identified in the Escherichia coli dnaB protein (Mr = 52,000) by partial proteolytic cleavage under nondenaturing conditions...
  38. Skokotas A, Wrobleski M, Hill T. Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli. J Biol Chem. 1994;269:20446-55 pubmed
    ..4100-fold lower affinity for Ter sites than wild type Tus and was unable to halt DNA replication in vivo or inhibit DnaB-catalyzed strand displacement in an in vitro helicase assay...
  39. Skarstad K, Wold S. The speed of the Escherichia coli fork in vivo depends on the DnaB:DnaC ratio. Mol Microbiol. 1995;17:825-31 pubmed
    The DnaC protein is required for loading the DnaB helicase at oriC. Thus DnaC promotes the formation of the pre-replication complex, but must leave the complex in order for the DnaB protein to function as a helicase...
  40. Den Blaauwen T, Aarsman M, Wheeler L, Nanninga N. Pre-replication assembly of E. coli replisome components. Mol Microbiol. 2006;62:695-708 pubmed
    The localization of SeqA, thymidylate synthase, DnaB (helicase) and the DNA polymerase components alpha and tau, has been studied by immunofluorescence microscopy. The origin has been labelled through GFP-LacI bound near oriC...
  41. Michel Reydellet N, Woodrow K, Swartz J. Increasing PCR fragment stability and protein yields in a cell-free system with genetically modified Escherichia coli extracts. J Mol Microbiol Biotechnol. 2005;9:26-34 pubmed
    ..With this system, it was possible to rapidly express a range of cytoplasmic and periplasmic proteins. ..
  42. Kaplan D, O Donnell M. Twin DNA pumps of a hexameric helicase provide power to simultaneously melt two duplexes. Mol Cell. 2004;15:453-65 pubmed
    b>DnaB is the primary replicative helicase in Escherichia coli. We show here that DnaB can unwind two duplex arms simultaneously for an extended distance provided that two protein rings are positioned on opposite sides of the duplex arms...
  43. Biswas E, Chen P, Biswas S. Modulation of enzymatic activities of Escherichia coli DnaB helicase by single-stranded DNA-binding proteins. Nucleic Acids Res. 2002;30:2809-16 pubmed
    The modulation of enzymatic activities of Escherichia coli DnaB helicase by homologous and heterologous single-stranded DNA-binding proteins (SSBs) and its DNA substrates were analyzed...
  44. Tanner N, Hamdan S, Jergic S, Loscha K, Schaeffer P, Dixon N, et al. Single-molecule studies of fork dynamics in Escherichia coli DNA replication. Nat Struct Mol Biol. 2008;15:170-6 pubmed publisher
    ..When coupled to the replicative helicase DnaB, Pol III mediates leading-strand synthesis with a processivity of 10...
  45. Akaboshi E. Recognition of sequence-directed structure of the ssDNA backbone by nucleases. Biochem Biophys Res Commun. 1999;256:532-6 pubmed
    ..The digestion patterns were examined in relation to previous results of biochemical and crystallographic studies on dsDNA, and showed broad agreement. Endonuclease I cleaved ssDNA at sites corresponding to bent sites in dsDNA. ..
  46. Bull H, Hayes S. The grpD55 locus of Escherichia coli appears to be an allele of dnaB. Mol Gen Genet. 1996;252:755-60 pubmed
    ..The mutation was found to cotransduce with malF3089:: Tn10 (at approximately 91.5 min) and a dnaB-expressing plasmid was able to complement fully the grpD55 defect in lambda replication...
  47. Soultanas P. The bacterial helicase-primase interaction: a common structural/functional module. Structure. 2005;13:839-44 pubmed
    ..An attractive structural model with direct biological significance for the function of this complex and also for the development of new antibacterial compounds is examined. ..
  48. Galletto R, Jezewska M, Bujalowski W. Unzipping mechanism of the double-stranded DNA unwinding by a hexameric helicase: quantitative analysis of the rate of the dsDNA unwinding, processivity and kinetic step-size of the Escherichia coli DnaB helicase using rapid quench-flow method. J Mol Biol. 2004;343:83-99 pubmed
    Kinetics of the double-stranded (ds) DNA unwinding by the Escherichia coli replicative helicase DnaB protein has been examined under single-turnover conditions using the chemical quench-flow technique...
  49. Weigel C, Seitz H. Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC. Mol Microbiol. 2002;46:1149-56 pubmed
    We analysed the enzymatic activity (strand dis-placement) of the Escherichia coli DnaB helicase on a mirror-image pair of oligonucleotide-based substrates mimicking the unwound replication origin oriC...
  50. Kaplan D, Bastia D. Mechanisms of polar arrest of a replication fork. Mol Microbiol. 2009;72:279-85 pubmed publisher
    ..In yeast, terminator sequences are present in rDNA non-transcribed spacers and a region immediately preceding the mating type switch locus Mat1, and the mechanism of polar arrest at these regions is beginning to be elucidated. ..