hfq

Summary

Gene Symbol: hfq
Description: global sRNA chaperone; HF-I, host factor for RNA phage Q beta replication
Alias: ECK4168, JW4130
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs
    Isabella Moll
    Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    RNA 9:1308-14. 2003
  2. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
  3. pmc Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs
    Peter J Mikulecky
    Department of Chemistry, Indiana University, 800 E Kirkwood Avenue, Bloomington, Indiana 47405, USA
    Nat Struct Mol Biol 11:1206-14. 2004
  4. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
  5. pmc The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation
    Marc Folichon
    UPR CNRS no 9073, conventionnée avec l Université Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 75005 Paris, France
    Nucleic Acids Res 31:7302-10. 2003
  6. ncbi Escherichia coli Hfq binds A18 and DsrA domain II with similar 2:1 Hfq6/RNA stoichiometry using different surface sites
    Xueguang Sun
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Biochemistry 45:4875-87. 2006
  7. ncbi Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030
    Mol Microbiol 13:35-49. 1994
  8. ncbi The bacterial Sm-like protein Hfq: a key player in RNA transactions
    Poul Valentin-Hansen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 51:1525-33. 2004
  9. ncbi Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 61:1013-22. 2006
  10. pmc Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli
    Eric Guisbert
    Department of Biochemistry, University of California at San Francisco, San Francisco, CA 94158, USA
    J Bacteriol 189:1963-73. 2007

Research Grants

  1. REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRA
    DARREN SLEDJESKI; Fiscal Year: 2002
  2. X-RAY STUDIES ON BACTERIAL MDR REGULATORS
    Richard Brennan; Fiscal Year: 2008

Scientific Experts

  • Vladimir R Kaberdin
  • RICHARD GERALD BRENNAN
  • Carin K Vanderpool
  • Klas I Udekwu
  • Veronique Arluison
  • Hiroji Aiba
  • R Gary Sawers
  • M B Garber
  • Regine Hengge
  • Marion Scheibe
  • A J Carpousis
  • Poul Valentin-Hansen
  • G Storz
  • M Wachi
  • R A Lease
  • Darren D Sledjeski
  • Shoshy Altuvia
  • Branislav Vecerek
  • Udo Blasi
  • Susan Gottesman
  • Isabella Moll
  • Teppei Morita
  • Marc Folichon
  • Eric Massé
  • Elisabeth Sonnleitner
  • Hiroshi Kawamoto
  • Eliane Hajnsdorf
  • Philippe Regnier
  • H C Tsui
  • Aixia Zhang
  • Bijoy K Mohanty
  • Véronique Douchin
  • Anders Aamann Rasmussen
  • Andrew L Feig
  • Taylor B Updegrove
  • Razika Hussein
  • Makhlouf Rabhi
  • Kyung Moon
  • D Schuppli
  • Peter J Mikulecky
  • Taras Afonyushkin
  • Cristin C Brescia
  • M E Winkler
  • Toby Soper
  • Yinghua Zhang
  • Sarah A Woodson
  • T A Azam
  • Valerie J Carabetta
  • Anne Marie Hansen
  • Ambre Jousselin
  • Todd M Link
  • F Repoila
  • Roger M Wartell
  • Renee Schroeder
  • Nikolai Windbichler
  • Anne Marie Holland
  • O Vytvytska
  • Toby J Soper
  • Thorleif Møller
  • Taewoo Lee
  • Ayako Takada
  • Sandra C Viegas
  • Karl M Thompson
  • Eric Guisbert
  • Virgil A Rhodius
  • Jörg Vogel
  • Brice Felden
  • Johannes H Urban
  • A Ishihama
  • Nadim Majdalani
  • Sidney R Kushner
  • Ashima K Sharma
  • Xueguang Sun
  • Katarzyna Ziolkowska
  • Jesper Johansen
  • Yong Zhang
  • A Takada
  • Maria Antal
  • Olivier Pellegrini
  • Thomas A Geissmann
  • Thomas Franch
  • Claude Sauter
  • Jacques Le Derout
  • A Muffler
  • Maxim V Sukhodolets
  • Karen M Wassarman
  • H Weber
  • S Gottesman
  • Regine Hengge-Aronis
  • I M Vassilieva

Detail Information

Publications104 found, 100 shown here

  1. pmc Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs
    Isabella Moll
    Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    RNA 9:1308-14. 2003
    The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase...
  2. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    b>Hfq-binding antisense small RNAs of Escherichia coli, SgrS and RyhB, mediate the destabilization of target mRNAs in an RNase E-dependent manner...
  3. pmc Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs
    Peter J Mikulecky
    Department of Chemistry, Indiana University, 800 E Kirkwood Avenue, Bloomington, Indiana 47405, USA
    Nat Struct Mol Biol 11:1206-14. 2004
    The bacterial Sm-like protein Hfq facilitates RNA-RNA interactions involved in post-transcriptional regulation of the stress response. Specifically, Hfq helps pair noncoding RNAs (ncRNAs) with complementary regions of target mRNAs...
  4. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ..RyhB requires the RNA-binding protein, Hfq, for activity...
  5. pmc The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation
    Marc Folichon
    UPR CNRS no 9073, conventionnée avec l Université Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 75005 Paris, France
    Nucleic Acids Res 31:7302-10. 2003
    The Hfq protein, which shares sequence and structural homology with the Sm and Lsm proteins, binds to various RNAs, primarily recognizing AU-rich single-stranded regions...
  6. ncbi Escherichia coli Hfq binds A18 and DsrA domain II with similar 2:1 Hfq6/RNA stoichiometry using different surface sites
    Xueguang Sun
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Biochemistry 45:4875-87. 2006
    b>Hfq is a RNA-binding protein in Escherichia coli that plays an essential role in post-transcriptional regulation of mRNAs by facilitating pairing of noncoding RNAs (ncRNAs) to mRNA target sites. Recent work has provided evidence that E...
  7. ncbi Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030
    Mol Microbiol 13:35-49. 1994
    The region immediately downstream from the miaA tRNA modification gene at 94.8 min contains the hfq gene and the hflA region, which are important in the bacteriophage Q beta and lambda life cycles...
  8. ncbi The bacterial Sm-like protein Hfq: a key player in RNA transactions
    Poul Valentin-Hansen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 51:1525-33. 2004
    The conserved RNA-binding protein Hfq, originally discovered in Escherichia coli as a host factor for Qbeta replicase, has emerged as a pleiotropic regulator that modulates the stability or the translation of an increasing number of ..
  9. ncbi Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 61:1013-22. 2006
    SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress...
  10. pmc Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli
    Eric Guisbert
    Department of Biochemistry, University of California at San Francisco, San Francisco, CA 94158, USA
    J Bacteriol 189:1963-73. 2007
    b>Hfq, a chaperone for small noncoding RNAs, regulates many processes in Escherichia coli, including the sigma(S)-mediated general stress response...
  11. ncbi The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer
    Veronique Arluison
    Institut de Biologie Physico Chimique CNRS UPR 9073 conventionnée avec l université Paris 7, Paris, France
    Eur J Biochem 271:1258-65. 2004
    The Hfq (Host factor 1) polypeptide is a nucleic acid binding protein involved in the synthesis of many polypeptides. Hfq particularly affects the translation and the stability of several RNAs...
  12. pmc Characterization of the role of ribonucleases in Salmonella small RNA decay
    Sandra C Viegas
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
    Nucleic Acids Res 35:7651-64. 2007
    ..The stability of CsrB and CsrC seemed to be independent of the RNA chaperone, Hfq, whereas the decay of SraL and MicA was Hfq-dependent...
  13. doi On the facultative requirement of the bacterial RNA chaperone, Hfq
    Ambre Jousselin
    Universite de Rennes I, Inserm U835, UPRES EA2311, Biochimie Pharmaceutique, 35043 Rennes, France
    Trends Microbiol 17:399-405. 2009
    The pleiotropic post-transcriptional regulator Hfq is an RNA chaperone that facilitates pairing interactions between small regulatory RNAs (sRNAs) and their mRNA targets in several bacteria...
  14. pmc The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli
    A Muffler
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 179:297-300. 1997
    The hfq-encoded RNA-binding protein HF-I has long been known as a host factor for phage Qbeta RNA replication and has recently been shown to be essential for translation of rpoS, which encodes the sigmaS subunit of RNA polymerase...
  15. pmc Hfq is necessary for regulation by the untranslated RNA DsrA
    D D Sledjeski
    Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614, USA
    J Bacteriol 183:1997-2005. 2001
    ..b>Hfq is an E. coli protein necessary for the in vitro and in vivo replication of the RNA phage Qbeta...
  16. ncbi Structural Modelling of the Sm-like Protein Hfq from Escherichia coli
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073, 13 rue P et M Curie, 75005 Paris, France
    J Mol Biol 320:705-12. 2002
    The Hfq polypeptide of Escherichia coli is a nucleic acid-binding protein involved in the expression of many proteins...
  17. ncbi A small bacterial RNA regulates a putative ABC transporter
    Maria Antal
    Biochimie Pharmaceutique, Universite de Rennes I, UPRES Jeune Equipe 2311, ESPRI INSERM, 2 Avenue du Professeur Leon Bernard, 35043 Rennes, France
    J Biol Chem 280:7901-8. 2005
    ..In vivo, RydC binds to the RNA-binding protein Hfq, and it is unstable when Hfq is absent...
  18. ncbi Three-dimensional structures of fibrillar Sm proteins: Hfq and other Sm-like proteins
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073 conventionnee avec l université Paris 7, 13 rue P et M Curie, 75005 Paris, France
    J Mol Biol 356:86-96. 2006
    b>Hfq is a nucleic acid-binding protein that functions as a global regulator of gene expression by virtue of its interactions with several small, non-coding RNA species...
  19. ncbi Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins
    Jesper Johansen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
    J Mol Biol 364:1-8. 2006
    ..Here, we show that two Hfq-binding small RNAs, MicA and RybB, are under positive control of sigmaE...
  20. pmc The C-terminal domain of Escherichia coli Hfq is required for regulation
    Branislav Vecerek
    Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    Nucleic Acids Res 36:133-43. 2008
    The Escherichia coli RNA chaperone Hfq is involved in riboregulation of target mRNAs by small trans-encoded non-coding (ncRNAs)...
  21. ncbi The host factor required for RNA phage Qbeta RNA replication in vitro. Intracellular location, quantitation, and purification by polyadenylate-cellulose chromatography
    G G Carmichael
    J Biol Chem 250:3607-612. 1975
    ....
  22. pmc Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta
    M Kajitani
    Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan
    J Bacteriol 176:531-4. 1994
    The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome...
  23. ncbi The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli
    A Muffler
    Department of Biology, University of Konstanz, Germany
    Genes Dev 10:1143-51. 1996
    ..Here, we identify the hfq-encoded RNA-binding protein HF-I, which has been known previously only as a host factor for the replication of ..
  24. pmc Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma32-specific promoters during heat shock
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030 1501, USA
    J Bacteriol 178:5719-31. 1996
    The amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of Escherichia coli contains genes that are important for diverse cellular functions, including DNA mismatch repair (mutL), tRNA modification (miaA), pleiotropic regulation (hfq), and ..
  25. ncbi DNA binding properties of the hfq gene product of Escherichia coli
    A Takada
    Department of Bioengineering, Tokyo Institute of Technology, Midori ku, Yokohama, Japan
    Biochem Biophys Res Commun 236:576-9. 1997
    ..N-terminal amino acid residues of the 15-kDa protein was identical with the host factor I (HF-I) encoded by the hfq gene at 95 min on the Escherichia coli chromosome map, which is known to be required for bacteriophage Qbeta ..
  26. pmc Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030 1501, USA
    J Bacteriol 179:7476-87. 1997
    ..we tested whether the amounts of MutS, MutL, and MutH were regulated by two global regulators, RpoS (sigma38) and Hfq (HF-I [putative RNA chaperone]), which are involved in stationary-phase transition...
  27. pmc The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
    A Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:6061-8. 1998
    ..This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I)...
  28. pmc Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability
    O Vytvytska
    Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Dr Bohr Gasse 9, A 1030 Vienna, Austria
    Proc Natl Acad Sci U S A 95:14118-23. 1998
    ..An RNA-binding protein with affinity for the 5' untranslated region ompA was purified and identified as Hfq, a host factor initially recognized for its function in phage Qbeta replication...
  29. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  30. ncbi Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant
    M Wachi
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    Biochem Biophys Res Commun 264:525-9. 1999
    We found that 82- and 76-kDa proteins in the outer-membrane fractions were overproduced in the hfq::cat mutant cells when grown in synthetic media...
  31. ncbi Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Biol Chem 274:33105-13. 1999
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  32. pmc Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I
    E Hajnsdorf
    Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 97:1501-5. 2000
    ..Here, we show that host factor Hfq is also involved in poly(A) tail metabolism...
  33. ncbi Post-transcriptional control by global regulators of gene expression in bacteria
    T Nogueira
    Institut de Biologie Physico Chimique, Paris, 75005, France
    Curr Opin Microbiol 3:154-8. 2000
    ..This is the case for Hfq (HF-1), which is involved in the regulation of an increasing number of genes in Escherichia coli, and CsrA (RsmA) ..
  34. pmc Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding
    O Vytvytska
    Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Vienna, Austria
    Genes Dev 14:1109-18. 2000
    ..Recently, host factor I (Hfq/HF1) was shown to be involved in the regulation of ompA mRNA stability under slow growth conditions...
  35. ncbi Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Genes Cells 5:613-26. 2000
    ..The intracellular localization of these proteins in E. coli could be predicted from these data, but no attempt has been made thus far to directly observe the intracellular distribution of the DNA-binding proteins...
  36. ncbi The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 9:11-22. 2002
    The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhlA and rpoS mRNA translation...
  37. ncbi Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction
    Thorleif Møller
    Department of Biochemistry and Molecular Biology, University of Southern Denmark Odense, Campusvej 55, DK 5230 M, Odense, Denmark
    Mol Cell 9:23-30. 2002
    The bacterial Hfq protein modulates the stability or the translation of mRNAs and has recently been shown to interact with small regulatory RNAs in E. coli...
  38. ncbi Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa
    Elisabeth Sonnleitner
    Institute of Microbiology and Genetics, Vienna Biocentre, Dr Bohrgasse 9, 1030 Vienna, Austria
    Microbiology 148:883-91. 2002
    The 102 aa Hfq protein of Escherichia coli (Hfq(Ec)) was first described as a host factor required for phage Qbeta replication. More recently, Hfq was shown to affect the stability of several E...
  39. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    In prokaryotes, Hfq regulates translation by modulating the structure of numerous RNA molecules by binding preferentially to A/U-rich sequences...
  40. pmc Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase
    Regine Hengge-Aronis
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Microbiol Mol Biol Rev 66:373-95, table of contents. 2002
    ..rpoS translation is controlled by several proteins (Hfq and HU) and small regulatory RNAs that probably affect the secondary structure of rpoS mRNA...
  41. pmc Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure
    Cristin C Brescia
    Department of Microbiology and Immunology, Medical College of Ohio, Toledo 43614, USA
    RNA 9:33-43. 2003
    ..DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo...
  42. pmc RNA chaperone activity of the Sm-like Hfq protein
    Isabella Moll
    Institute of Microbiology and Genetics, Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    EMBO Rep 4:284-9. 2003
    The Escherichia coli Sm-like host factor I (Hfq) protein is thought to function in post-transcriptional regulation by modulating the function of small regulatory RNAs. Hfq also interferes with ribosome binding on E...
  43. ncbi Regulatory roles for small RNAs in bacteria
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
    Curr Opin Microbiol 6:120-4. 2003
    ..These include many that require the RNA-binding protein Hfq for their activity; most of these RNAs act by pairing with their target mRNAs...
  44. ncbi Small non-coding RNAs, co-ordinators of adaptation processes in Escherichia coli: the RpoS paradigm
    F Repoila
    UMR960 INRA ENVT, Laboratoire de Microbiologie Moléculaire, 23 Chemin des Capelles, 31076 Toulouse Cedex, France
    Mol Microbiol 48:855-61. 2003
    ..Environmental cues affect the synthesis and stability of specific sRNAs, resulting in specific sRNA-dependent translational control...
  45. ncbi Interaction of Escherichia coli RNA polymerase with the ribosomal protein S1 and the Sm-like ATPase Hfq
    Maxim V Sukhodolets
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 42:8022-34. 2003
    We report evidence that ribosomal protein S1 and nucleic acid-binding protein Hfq copurify in molar ratios with RNA polymerase (RNAP)...
  46. pmc Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs
    Jacques Le Derout
    UPR 9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Nucleic Acids Res 31:4017-23. 2003
    ..and distribution of poly(A) tails at the 3'-end of rpsO transcripts in vivo in the presence and absence of Hfq, a host factor that in vitro stimulates the activity of PAP I, and found that Hfq affects all three parameters...
  47. pmc Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli
    Claude Sauter
    European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 31:4091-8. 2003
    The Hfq protein was discovered in Escherichia coli in the early seventies as a host factor for the Qbeta phage RNA replication...
  48. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    ..RyhB requires the RNA chaperone Hfq. In the absence of Hfq, RyhB is unstable, even when general transcription is inhibited; degradation is dependent ..
  49. ncbi Interaction of the RNA chaperone Hfq with mRNAs: direct and indirect roles of Hfq in iron metabolism of Escherichia coli
    Branislav Vecerek
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University Department at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    Mol Microbiol 50:897-909. 2003
    The Escherichia coli Sm-like host factor I (Hfq) is thought to play direct and indirect roles in post-transcriptional regulation by targeting small regulatory RNAs and mRNAs...
  50. ncbi Global analysis of small RNA and mRNA targets of Hfq
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Development, Bethesda MD 20892, USA
    Mol Microbiol 50:1111-24. 2003
    b>Hfq, a bacterial member of the Sm family of RNA-binding proteins, is required for the action of many small regulatory RNAs that act by basepairing with target mRNAs. Hfq binds this family of small RNAs efficiently...
  51. pmc Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator
    Thomas A Geissmann
    Institut Jacques Monod, CNRS Universités Paris 6 et Paris 7, Paris, France
    EMBO J 23:396-405. 2004
    The Sm-like protein Hfq is involved in post-transcriptional regulation by small, noncoding RNAs in Escherichia coli that act by base pairing...
  52. ncbi Controlling mRNA stability and translation with small, noncoding RNAs
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    Curr Opin Microbiol 7:140-4. 2004
    ..Basepairing between these regulatory RNAs and their target mRNAs requires the Sm-like Hfq protein which most likely functions as an RNA chaperone to increase RNA unfolding or local target RNA concentration...
  53. ncbi Functional effects of variants of the RNA chaperone Hfq
    Elisabeth Sonnleitner
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9 4, 1030 Vienna, Austria
    Biochem Biophys Res Commun 323:1017-23. 2004
    The ring-shaped RNA chaperone Hfq has recently received much attention owing to its multiple roles in RNA metabolism...
  54. ncbi The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 54:905-20. 2004
    ..Here we show that the inactivation of Hfq (hfq), an abundant RNA-binding protein, leads to the reduction in the ability of PAP I to add poly(A) tails at the ..
  55. ncbi Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system
    Carin K Vanderpool
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 54:1076-89. 2004
    ..a small non-coding RNA in Escherichia coli that was identified by its ability to bind tightly to the RNA chaperone Hfq. This study reports the role of RyaA in mediating the cellular response to glucose-specific phosphoenolypyruvate ..
  56. pmc Implication of membrane localization of target mRNA in the action of a small RNA: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:328-38. 2005
    ..The stimulation of ptsG-crp mRNA degradation was completely eliminated when either the hfq or sgrS gene is inactivated...
  57. ncbi Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails
    Marc Folichon
    UPR CNRS 9073, conventionnée avec l Université Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    FEBS J 272:454-63. 2005
    The bacterial Lsm protein, host factor I (Hfq), is an RNA chaperone involved in many types of RNA transactions such as replication and stability, control of small RNA activity and polyadenylation...
  58. pmc Translational autocontrol of the Escherichia coli hfq RNA chaperone gene
    Branislav Vecerek
    Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Austria
    RNA 11:976-84. 2005
    The conserved bacterial RNA chaperone Hfq has been shown to play an important role in post-transcriptional regulation. Here, we demonstrate that Hfq synthesis is autoregulated at the translational level...
  59. pmc Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
    Genes Dev 19:2355-66. 2005
    ..Regulation of ompA (outer membrane protein A), previously attributed to Hfq/mRNA binding, is lost upon deletion of the micA gene, whereas overexpression of MicA inhibits the synthesis of OmpA...
  60. ncbi Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control
    Anders Aamann Rasmussen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 58:1421-9. 2005
    ..b>Hfq binds to this region, is essential for controlling the stability and has been suggested to directly regulate ompA ..
  61. pmc Hfq variant with altered RNA binding functions
    Katarzyna Ziolkowska
    Régulation de l Expression Génétique chez les Microorganismes, UPR CNRS no 9073 conventionnée avec l Université Paris 7, Denis Diderot, Paris, France
    Nucleic Acids Res 34:709-20. 2006
    The interaction between Hfq and RNA is central to multiple regulatory processes...
  62. ncbi Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli
    Véronique Douchin
    Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
    J Biol Chem 281:12253-9. 2006
    ..The suppressor encodes a novel small RNA, RseX. Its activity required the RNA-binding protein Hfq. We used the property that small RNAs are often involved in RNA-RNA interactions to capture RseX putative partners; ..
  63. pmc Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 103:4858-63. 2006
    Stress-induced Hfq-binding small RNAs of Escherichia coli, SgrS and RyhB, down-regulate the expression of target mRNAs through base-pairing. These small RNAs form ribonucleoprotein complexes with Hfq and RNase E...
  64. ncbi Induction of expression of hfq by DksA is essential for Shigella flexneri virulence
    Ashima K Sharma
    Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, TX 78712, USA
    Mol Microbiol 62:469-79. 2006
    b>Hfq plays an important role in cellular physiology by regulating the expression of several genes. Hfq synthesis in Escherichia coli is subject to auto-repression at translational level...
  65. pmc Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073 conventionnee avec l université Paris 7, 13 rue P et M Curie, 75005 Paris, France
    Nucleic Acids Res 35:999-1006. 2007
    b>Hfq protein is vital for the function of many non-coding small (s)RNAs in bacteria but the mechanism by which Hfq facilitates the function of sRNA is still debated...
  66. pmc Sigma E controls biogenesis of the antisense RNA MicA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, S 75124 Uppsala, Sweden
    Nucleic Acids Res 35:1279-88. 2007
    ..This regulation is Hfq-dependent and occurs by MicA-dependent translational inhibition which facilitates mRNA decay...
  67. ncbi Mechanism of RNA silencing by Hfq-binding small RNAs
    Hiroji Aiba
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Curr Opin Microbiol 10:134-9. 2007
    ..small RNAs SgrS and RyhB in Escherichia coli form a specific ribonucleoprotein complex with RNAse E and Hfq resulting in translation inhibition, RNAse E-dependent degradation of target mRNAs...
  68. ncbi Hfq structure, function and ligand binding
    Richard G Brennan
    Department of Biochemistry and Molecular Biology, Unit 1000 University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard Houston, TX 77030 4009, USA
    Curr Opin Microbiol 10:125-33. 2007
    Recent studies on Hfq have provided a deeper understanding of the multiple functions of this pleiotropic post-transcriptional regulator. Insights into the mechanism of Hfq action have come from a variety of approaches...
  69. pmc SigmaE regulates and is regulated by a small RNA in Escherichia coli
    Karl M Thompson
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 189:4243-56. 2007
    RybB is a small, Hfq-binding noncoding RNA originally identified in a screen of conserved intergenic regions in Escherichia coli...
  70. ncbi Identification of bacterial small non-coding RNAs: experimental approaches
    Shoshy Altuvia
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Curr Opin Microbiol 10:257-61. 2007
    ..Consequently, unique sRNAs such as those that are species-specific, sRNA genes that are transcribed under unique conditions or genes located on the antisense strand of protein-encoding genes, were probably missed...
  71. pmc Hfq stimulates the activity of the CCA-adding enzyme
    Marion Scheibe
    Institute for Biochemistry, University of Leipzig, Brüderstr, 34, 04103 Leipzig, Germany
    BMC Mol Biol 8:92. 2007
    The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP)...
  72. pmc The RNA binding protein Hfq interacts specifically with tRNAs
    Taewoo Lee
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    RNA 14:514-23. 2008
    b>Hfq is an RNA binding protein that has been studied extensively for its role in the biology of small noncoding RNAs (ncRNAs) in bacteria, where it facilitates post-transcriptional gene regulation during stress responses...
  73. ncbi Isolation of small RNA-binding proteins from E. coli: evidence for frequent interaction of RNAs with RNA polymerase
    Nikolai Windbichler
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 5:30-40. 2008
    ..We consistently obtained RNA polymerase betasubunit, host factor Hfq and ribosomal protein S1 as sRNA-binding proteins in addition to several other factors...
  74. pmc The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA
    Toby J Soper
    Program in Cellular, Molecular, Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, Maryland 21218 2685, USA
    RNA 14:1907-17. 2008
    ..regulate the response of bacteria to environmental stress in conjunction with the Sm-like RNA binding protein Hfq. DsrA sRNA stimulates translation of the RpoS stress response factor in Escherichia coli by base-pairing with the 5'..
  75. pmc Structure of Escherichia coli Hfq bound to polyriboadenylate RNA
    Todd M Link
    Department of Biochemistry and Molecular Biology, University of Texas, MD Anderson Cancer Center, Houston, TX 77030 4009, USA
    Proc Natl Acad Sci U S A 106:19292-7. 2009
    b>Hfq is a small, highly abundant hexameric protein that is found in many bacteria and plays a critical role in mRNA expression and RNA stability...
  76. pmc Translational activation of rpoS mRNA by the non-coding RNA DsrA and Hfq does not require ribosome binding
    Branislav Vecerek
    Max F Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    Nucleic Acids Res 38:1284-93. 2010
    ..the stationary phase sigma-factor, sigma(S), involves the small regulatory RNA (sRNA) DsrA and the RNA chaperone Hfq. The Hfq-mediated DsrA-rpoS interaction relieves an intramolecular secondary structure that impedes ribosome access ..
  77. pmc Positive regulation by small RNAs and the role of Hfq
    Toby Soper
    Johns Hopkins University, Program in Cell, Molecular, Developmental Biology and Biophysics, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:9602-7. 2010
    ..of gene expression by pairing with mRNAs; in Escherichia coli, this regulation often requires the RNA chaperone Hfq. Three small regulatory RNAs (sRNAs), DsrA, RprA, and ArcZ, positively regulate translation of the sigma factor ..
  78. pmc Disruption of small RNA signaling caused by competition for Hfq
    Razika Hussein
    Department of Integrative Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:1110-5. 2011
    ..At the center of this large sRNA regulatory network is the Hfq protein...
  79. pmc The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators
    Makhlouf Rabhi
    Centre de Biophysique Moleculaire, CNRS UPR4301, rue Charles Sadron, Orleans cedex 2, France
    EMBO J 30:2805-16. 2011
    ..Here, we show that the general post-transcriptional regulatory protein Hfq associates with Rho to regulate Rho function...
  80. doi The influence of Escherichia coli Hfq mutations on RNA binding and sRNA•mRNA duplex formation in rpoS riboregulation
    Taylor B Updegrove
    School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Biochim Biophys Acta 1809:532-40. 2011
    The Escherichia coli RNA binding protein Hfq plays an important role in regulating mRNA translation through its interactions with small non-coding RNAs (sRNAs) and specific mRNAs sites...
  81. doi Competition among Hfq-binding small RNAs in Escherichia coli
    Kyung Moon
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Microbiol 82:1545-62. 2011
    ..RNAs (sRNAs) regulate translation and mRNA stability by pairing with target mRNAs, dependent upon the RNA chaperone Hfq. Hfq, related to the Lsm/Sm families of splicing proteins, binds the sRNAs and stabilizes them in vivo and ..
  82. pmc Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations
    D M Connolly
    Department of Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611
    J Bacteriol 173:1711-21. 1991
    ..Together, these observations support the idea that the ms2i6A-37 tRNA modification acts as a physiological switch that modulates spontaneous mutation frequency and other metabolic functions...
  83. pmc Identification and sequence determination of the host factor gene for bacteriophage Q beta
    M Kajitani
    Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan
    Nucleic Acids Res 19:1063-6. 1991
    ..The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and ..
  84. ncbi The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030
    Mol Microbiol 11:189-202. 1994
    ..Together, these results suggest that E. coli contains a second amidase possibly involved in cell-wall hydrolysis, septation, or recycling, and that transcription of this amidase is directly linked to a gene central for DNA repair...
  85. ncbi Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030, USA
    Biochimie 76:1168-77. 1994
    The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (..
  86. pmc The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain
    J A Noble
    Cetus Corporation, Emeryville, CA 94608
    Proc Natl Acad Sci U S A 90:10866-70. 1993
    ..We hypothesize that HflK and HflC constitute a distinct membrane-bound protease whose activity may be modulated by HflX GTPase...
  87. ncbi Strongly reduced phage Qbeta replication, but normal phage MS2 replication in an Escherichia coli K12 mutant with inactivated Qbeta host factor (hfq) gene
    Q Su
    Institut für Molekularbiologie Abt I, Universitat Zurich, Zurich, 8093, Switzerland
    Virology 227:211-4. 1997
    RNA phage production was observed in Escherichia coli hfq mutant strains transformed with plasmids containing genomic cDNA of phages Qbeta or MS2...
  88. ncbi Negative regulatory role of the Escherichia coli hfq gene in cell division
    A Takada
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    Biochem Biophys Res Commun 266:579-83. 1999
    We found that the hfq::cat mutant strain produced minicells at high frequency...
  89. pmc Identification of novel small RNAs using comparative genomics and microarrays
    K M Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 15:1637-51. 2001
    ..We discovered that many of these small RNAs interact with the RNA-binding protein Hfq, pointing to a global role of the Hfq protein in facilitating small RNA function...
  90. ncbi Cloning, purification, and crystallization of a bacterial gene expression regulator--Hfq protein from Escherichia coli
    I M Vassilieva
    Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia
    Biochemistry (Mosc) 67:1293-7. 2002
    Thermostable RNA-binding protein Hfq (also denoted HF1) is a multifunctional expression regulator of many bacterial genes...
  91. pmc Expression of fnr is constrained by an upstream IS5 insertion in certain Escherichia coli K-12 strains
    R Gary Sawers
    Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
    J Bacteriol 187:2609-17. 2005
    ..Remarkably, in strains bearing is5F the presence of Hfq prevents IS5-dependent transcriptional silencing of fnr expression...
  92. ncbi Identifying Hfq-binding small RNA targets in Escherichia coli
    Yong Zhang
    Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Biochem Biophys Res Commun 343:950-5. 2006
    The Hfq-binding small RNAs (sRNAs) have recently drawn much attention as regulators of translation in Escherichia coli...
  93. ncbi RNase E is required for induction of the glutamate-dependent acid resistance system in Escherichia coli
    Ayako Takada
    Department of Bioengineering, Tokyo Institute of Technology, Japan
    Biosci Biotechnol Biochem 71:158-64. 2007
    ..It is known that RNase E activity is somehow regulated by an RNA-binding protein Hfq, at least in some cases...
  94. ncbi Physiological consequences of small RNA-mediated regulation of glucose-phosphate stress
    Carin K Vanderpool
    Department of Microbiology, University of Illinois at Urbana Champaign B213, Chemical and Life Sciences Laboratories CLSL, MC 110 Urbana, IL 61801, USA
    Curr Opin Microbiol 10:146-51. 2007
    ..coli. The effect of SgrS on ptsG mRNA occurs through a base-pairing mechanism facilitated by the RNA chaperone Hfq. Other host factors required for the regulation by SgrS include the endonuclease RNase E and components of the RNA ..
  95. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  96. pmc Sm-like protein Hfq: location of the ATP-binding site and the effect of ATP on Hfq-- RNA complexes
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073 conventionnee avec l université Paris 7, 75005 Paris, France
    Protein Sci 16:1830-41. 2007
    ..Our earlier studies of the Sm-like Escherichia coli protein Hfq provided the first evidence indicating that Hfq is an ATP-binding protein...
  97. doi Evidence for extracellular control of RpoS proteolysis in Escherichia coli
    Anne Marie Holland
    Department of Microbiology and Immunology, Atlanta, GA, USA
    FEMS Microbiol Lett 286:50-9. 2008
    ..the RpoS'-'LacZ translational fusion in dsrA, rprA or dsrA/rprA mutant backgrounds, but did not activate in an hfq mutant background...
  98. pmc Hfq affects the expression of the LEE pathogenicity island in enterohaemorrhagic Escherichia coli
    Anne Marie Hansen
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Mol Microbiol 73:446-65. 2009
    ..The LEE is subject to a complex regulatory network primarily targeting ler transcription. The RNA chaperone Hfq, implicated in post-transcriptional regulation, is an important virulence factor in many bacterial pathogens...
  99. ncbi Evidences of Hfq associates with tryptophanase and affects extracellular indole levels
    Yinghua Zhang
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Acta Biochim Biophys Sin (Shanghai) 41:709-17. 2009
    In this study, we observed a novel property of Escherichia coli Hfq protein: it possibly influenced extracellular indole levels...
  100. pmc The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli
    Valerie J Carabetta
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 191:6812-21. 2009
    ..Here we show that null mutations in pcnB, encoding poly(A) polymerase I (PAP I), and in hfq, encoding the RNA chaperone Hfq, suppress this toxicity...

Research Grants2

  1. REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRA
    DARREN SLEDJESKI; Fiscal Year: 2002
    ..2) To characterize the role of HF-I (hfq) an RNA binding protein that is essential for DsrA activity in vivo...
  2. X-RAY STUDIES ON BACTERIAL MDR REGULATORS
    Richard Brennan; Fiscal Year: 2008
    ..These data will be used in future structure-based drug design of novel drugs against pathogenic bacteria, including S. aureus, N. gonorrhoeae and potentially B. anthracis. ..