hfq

Summary

Gene Symbol: hfq
Description: global sRNA chaperone; HF-I, host factor for RNA phage Q beta replication
Alias: ECK4168, JW4130
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase...
  2. Vecerek B, Moll I, Afonyushkin T, Kaberdin V, Blasi U. Interaction of the RNA chaperone Hfq with mRNAs: direct and indirect roles of Hfq in iron metabolism of Escherichia coli. Mol Microbiol. 2003;50:897-909 pubmed
    The Escherichia coli Sm-like host factor I (Hfq) is thought to play direct and indirect roles in post-transcriptional regulation by targeting small regulatory RNAs and mRNAs...
  3. Guisbert E, Rhodius V, Ahuja N, Witkin E, Gross C. Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli. J Bacteriol. 2007;189:1963-73 pubmed
    b>Hfq, a chaperone for small noncoding RNAs, regulates many processes in Escherichia coli, including the sigma(S)-mediated general stress response...
  4. Thompson K, Rhodius V, Gottesman S. SigmaE regulates and is regulated by a small RNA in Escherichia coli. J Bacteriol. 2007;189:4243-56 pubmed
    RybB is a small, Hfq-binding noncoding RNA originally identified in a screen of conserved intergenic regions in Escherichia coli...
  5. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  6. Brescia C, Mikulecky P, Feig A, Sledjeski D. Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure. RNA. 2003;9:33-43 pubmed
    ..DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo...
  7. Udekwu K, Darfeuille F, Vogel J, Reimegård J, Holmqvist E, Wagner E. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes Dev. 2005;19:2355-66 pubmed
    ..Regulation of ompA (outer membrane protein A), previously attributed to Hfq/mRNA binding, is lost upon deletion of the micA gene, whereas overexpression of MicA inhibits the synthesis of OmpA...
  8. Wachi M, Takada A, Nagai K. Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant. Biochem Biophys Res Commun. 1999;264:525-9 pubmed
    We found that 82- and 76-kDa proteins in the outer-membrane fractions were overproduced in the hfq::cat mutant cells when grown in synthetic media...
  9. Muffler A, Traulsen D, Fischer D, Lange R, Hengge Aronis R. The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli. J Bacteriol. 1997;179:297-300 pubmed
    The hfq-encoded RNA-binding protein HF-I has long been known as a host factor for phage Qbeta RNA replication and has recently been shown to be essential for translation of rpoS, which encodes the sigmaS subunit of RNA polymerase...
  10. Morita T, Maki K, Aiba H. RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. Genes Dev. 2005;19:2176-86 pubmed
    b>Hfq-binding antisense small RNAs of Escherichia coli, SgrS and RyhB, mediate the destabilization of target mRNAs in an RNase E-dependent manner...

Detail Information

Publications62

  1. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase...
  2. Vecerek B, Moll I, Afonyushkin T, Kaberdin V, Blasi U. Interaction of the RNA chaperone Hfq with mRNAs: direct and indirect roles of Hfq in iron metabolism of Escherichia coli. Mol Microbiol. 2003;50:897-909 pubmed
    The Escherichia coli Sm-like host factor I (Hfq) is thought to play direct and indirect roles in post-transcriptional regulation by targeting small regulatory RNAs and mRNAs...
  3. Guisbert E, Rhodius V, Ahuja N, Witkin E, Gross C. Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli. J Bacteriol. 2007;189:1963-73 pubmed
    b>Hfq, a chaperone for small noncoding RNAs, regulates many processes in Escherichia coli, including the sigma(S)-mediated general stress response...
  4. Thompson K, Rhodius V, Gottesman S. SigmaE regulates and is regulated by a small RNA in Escherichia coli. J Bacteriol. 2007;189:4243-56 pubmed
    RybB is a small, Hfq-binding noncoding RNA originally identified in a screen of conserved intergenic regions in Escherichia coli...
  5. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  6. Brescia C, Mikulecky P, Feig A, Sledjeski D. Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure. RNA. 2003;9:33-43 pubmed
    ..DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo...
  7. Udekwu K, Darfeuille F, Vogel J, Reimegård J, Holmqvist E, Wagner E. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes Dev. 2005;19:2355-66 pubmed
    ..Regulation of ompA (outer membrane protein A), previously attributed to Hfq/mRNA binding, is lost upon deletion of the micA gene, whereas overexpression of MicA inhibits the synthesis of OmpA...
  8. Wachi M, Takada A, Nagai K. Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant. Biochem Biophys Res Commun. 1999;264:525-9 pubmed
    We found that 82- and 76-kDa proteins in the outer-membrane fractions were overproduced in the hfq::cat mutant cells when grown in synthetic media...
  9. Muffler A, Traulsen D, Fischer D, Lange R, Hengge Aronis R. The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli. J Bacteriol. 1997;179:297-300 pubmed
    The hfq-encoded RNA-binding protein HF-I has long been known as a host factor for phage Qbeta RNA replication and has recently been shown to be essential for translation of rpoS, which encodes the sigmaS subunit of RNA polymerase...
  10. Morita T, Maki K, Aiba H. RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. Genes Dev. 2005;19:2176-86 pubmed
    b>Hfq-binding antisense small RNAs of Escherichia coli, SgrS and RyhB, mediate the destabilization of target mRNAs in an RNase E-dependent manner...
  11. Mohanty B, Maples V, Kushner S. The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli. Mol Microbiol. 2004;54:905-20 pubmed
    ..Here we show that the inactivation of Hfq (hfq), an abundant RNA-binding protein, leads to the reduction in the ability of PAP I to add poly(A) tails at the ..
  12. Azam T, Hiraga S, Ishihama A. Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000;5:613-26 pubmed
    ..Here we show that the majority of Hfq is present in cytoplasm together with ribosomal proteins L7/L12 and RMF. The DNA-binding proteins of E...
  13. Mass E, Gottesman S. A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli. Proc Natl Acad Sci U S A. 2002;99:4620-5 pubmed publisher
    ..RyhB requires the RNA-binding protein, Hfq, for activity...
  14. Vytvytska O, Moll I, Kaberdin V, von Gabain A, Blasi U. Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes Dev. 2000;14:1109-18 pubmed
    ..Recently, host factor I (Hfq/HF1) was shown to be involved in the regulation of ompA mRNA stability under slow growth conditions...
  15. Le Derout J, Folichon M, Briani F, Deh G, R gnier P, Hajnsdorf E. Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs. Nucleic Acids Res. 2003;31:4017-23 pubmed
    ..and distribution of poly(A) tails at the 3'-end of rpsO transcripts in vivo in the presence and absence of Hfq, a host factor that in vitro stimulates the activity of PAP I, and found that Hfq affects all three parameters...
  16. Sauter C, Basquin J, Suck D. Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli. Nucleic Acids Res. 2003;31:4091-8 pubmed
    The Hfq protein was discovered in Escherichia coli in the early seventies as a host factor for the Qbeta phage RNA replication...
  17. Vytvytska O, Jakobsen J, Balcunaite G, Andersen J, Baccarini M, von Gabain A. Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability. Proc Natl Acad Sci U S A. 1998;95:14118-23 pubmed
    ..An RNA-binding protein with affinity for the 5' untranslated region ompA was purified and identified as Hfq, a host factor initially recognized for its function in phage Qbeta replication...
  18. Updegrove T, Wartell R. The influence of Escherichia coli Hfq mutations on RNA binding and sRNA•mRNA duplex formation in rpoS riboregulation. Biochim Biophys Acta. 2011;1809:532-40 pubmed publisher
    The Escherichia coli RNA binding protein Hfq plays an important role in regulating mRNA translation through its interactions with small non-coding RNAs (sRNAs) and specific mRNAs sites...
  19. Arluison V, Derreumaux P, Allemand F, Folichon M, Hajnsdorf E, R gnier P. Structural Modelling of the Sm-like Protein Hfq from Escherichia coli. J Mol Biol. 2002;320:705-12 pubmed
    The Hfq polypeptide of Escherichia coli is a nucleic acid-binding protein involved in the expression of many proteins...
  20. Antal M, Bordeau V, Douchin V, Felden B. A small bacterial RNA regulates a putative ABC transporter. J Biol Chem. 2005;280:7901-8 pubmed
    ..In vivo, RydC binds to the RNA-binding protein Hfq, and it is unstable when Hfq is absent...
  21. Moll I, Leitsch D, Steinhauser T, Bl si U. RNA chaperone activity of the Sm-like Hfq protein. EMBO Rep. 2003;4:284-9 pubmed publisher
    The Escherichia coli Sm-like host factor I (Hfq) protein is thought to function in post-transcriptional regulation by modulating the function of small regulatory RNAs. Hfq also interferes with ribosome binding on E...
  22. Kawamoto H, Morita T, Shimizu A, Inada T, Aiba H. Implication of membrane localization of target mRNA in the action of a small RNA: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli. Genes Dev. 2005;19:328-38 pubmed
    ..The stimulation of ptsG-crp mRNA degradation was completely eliminated when either the hfq or sgrS gene is inactivated...
  23. Zhang A, Altuvia S, Tiwari A, Argaman L, Hengge Aronis R, Storz G. The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. EMBO J. 1998;17:6061-8 pubmed
    ..This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I)...
  24. Kajitani M, Kato A, Wada A, Inokuchi Y, Ishihama A. Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta. J Bacteriol. 1994;176:531-4 pubmed
    The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome...
  25. Scheibe M, Bonin S, Hajnsdorf E, Betat H, Mörl M. Hfq stimulates the activity of the CCA-adding enzyme. BMC Mol Biol. 2007;8:92 pubmed
    The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP)...
  26. Hajnsdorf E, Regnier P. Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I. Proc Natl Acad Sci U S A. 2000;97:1501-5 pubmed
    ..Here, we show that host factor Hfq is also involved in poly(A) tail metabolism...
  27. Hengge Aronis R. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiol Mol Biol Rev. 2002;66:373-95, table of contents pubmed
    ..rpoS translation is controlled by several proteins (Hfq and HU) and small regulatory RNAs that probably affect the secondary structure of rpoS mRNA...
  28. Sledjeski D, Whitman C, Zhang A. Hfq is necessary for regulation by the untranslated RNA DsrA. J Bacteriol. 2001;183:1997-2005 pubmed
    ..b>Hfq is an E. coli protein necessary for the in vitro and in vivo replication of the RNA phage Qbeta...
  29. Geissmann T, Touati D. Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. EMBO J. 2004;23:396-405 pubmed
    The Sm-like protein Hfq is involved in post-transcriptional regulation by small, noncoding RNAs in Escherichia coli that act by base pairing...
  30. M ller T, Franch T, H jrup P, Keene D, B chinger H, Brennan R, et al. Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction. Mol Cell. 2002;9:23-30 pubmed
    The bacterial Hfq protein modulates the stability or the translation of mRNAs and has recently been shown to interact with small regulatory RNAs in E. coli...
  31. Mass E, Escorcia F, Gottesman S. Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli. Genes Dev. 2003;17:2374-83 pubmed publisher
    ..RyhB requires the RNA chaperone Hfq. In the absence of Hfq, RyhB is unstable, even when general transcription is inhibited; degradation is dependent ..
  32. Soper T, Woodson S. The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA. RNA. 2008;14:1907-17 pubmed publisher
    ..regulate the response of bacteria to environmental stress in conjunction with the Sm-like RNA binding protein Hfq. DsrA sRNA stimulates translation of the RpoS stress response factor in Escherichia coli by base-pairing with the 5'..
  33. Johansen J, Rasmussen A, Overgaard M, Valentin Hansen P. Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins. J Mol Biol. 2006;364:1-8 pubmed
    ..Here, we show that two Hfq-binding small RNAs, MicA and RybB, are under positive control of sigmaE...
  34. Rasmussen A, Eriksen M, Gilany K, Udesen C, Franch T, Petersen C, et al. Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Mol Microbiol. 2005;58:1421-9 pubmed
    ..b>Hfq binds to this region, is essential for controlling the stability and has been suggested to directly regulate ompA ..
  35. Zhang A, Wassarman K, Ortega J, Steven A, Storz G. The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs. Mol Cell. 2002;9:11-22 pubmed
    The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhlA and rpoS mRNA translation...
  36. Folichon M, Allemand F, Regnier P, Hajnsdorf E. Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails. FEBS J. 2005;272:454-63 pubmed
    The bacterial Lsm protein, host factor I (Hfq), is an RNA chaperone involved in many types of RNA transactions such as replication and stability, control of small RNA activity and polyadenylation...
  37. Sonnleitner E, Moll I, Bl si U. Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa. Microbiology. 2002;148:883-91 pubmed publisher
    The 102 aa Hfq protein of Escherichia coli (Hfq(Ec)) was first described as a host factor required for phage Qbeta replication. More recently, Hfq was shown to affect the stability of several E...
  38. Takada A, Wachi M, Kaidow A, Takamura M, Nagai K. DNA binding properties of the hfq gene product of Escherichia coli. Biochem Biophys Res Commun. 1997;236:576-9 pubmed
    ..N-terminal amino acid residues of the 15-kDa protein was identical with the host factor I (HF-I) encoded by the hfq gene at 95 min on the Escherichia coli chromosome map, which is known to be required for bacteriophage Qbeta ..
  39. Sharma A, Payne S. Induction of expression of hfq by DksA is essential for Shigella flexneri virulence. Mol Microbiol. 2006;62:469-79 pubmed
    b>Hfq plays an important role in cellular physiology by regulating the expression of several genes. Hfq synthesis in Escherichia coli is subject to auto-repression at translational level...
  40. Tsui H, Leung H, Winkler M. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol Microbiol. 1994;13:35-49 pubmed
    The region immediately downstream from the miaA tRNA modification gene at 94.8 min contains the hfq gene and the hflA region, which are important in the bacteriophage Q beta and lambda life cycles...
  41. Muffler A, Fischer D, Hengge Aronis R. The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli. Genes Dev. 1996;10:1143-51 pubmed
    ..Here, we identify the hfq-encoded RNA-binding protein HF-I, which has been known previously only as a host factor for the replication of ..
  42. Vecerek B, Beich Frandsen M, Resch A, Blasi U. Translational activation of rpoS mRNA by the non-coding RNA DsrA and Hfq does not require ribosome binding. Nucleic Acids Res. 2010;38:1284-93 pubmed publisher
    ..the stationary phase sigma-factor, sigma(S), involves the small regulatory RNA (sRNA) DsrA and the RNA chaperone Hfq. The Hfq-mediated DsrA-rpoS interaction relieves an intramolecular secondary structure that impedes ribosome access ..
  43. Mikulecky P, Kaw M, Brescia C, Takach J, Sledjeski D, Feig A. Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs. Nat Struct Mol Biol. 2004;11:1206-14 pubmed
    The bacterial Sm-like protein Hfq facilitates RNA-RNA interactions involved in post-transcriptional regulation of the stress response. Specifically, Hfq helps pair noncoding RNAs (ncRNAs) with complementary regions of target mRNAs...