Genomes and Genes
Gene Symbol: lacI
Description: lactose-inducible lac operon transcriptional repressor
Alias: ECK0342, JW0336
Species: Escherichia coli str. K-12 substr. MG1655
Publications115 found, 100 shown here
- The lac repressorMitchell Lewis
School of Medicine, University of Pennsylvania, 813 Stellar Chance Building, Philadelphia, PA 19104 6059, USA
C R Biol 328:521-48. 2005..The structures of lac repressor, bound to its operator and inducer, have also been invaluable for interpreting a plethora of biochemical and genetic data...
- Inferring the in vivo looping properties of DNALeonor Saiz
Integrative Biological Modeling Laboratory, Computational Biology Program, Memorial Sloan Kettering Cancer Center, 307 East 63rd Street, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:17642-5. 2005..These unexpected results can confer to the physical properties of DNA a more prominent role at shaping the properties of gene regulation than previously thought...
- Analysis of in-vivo LacR-mediated gene repression based on the mechanics of DNA loopingYongli Zhang
Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America
PLoS ONE 1:e136. 2006....
- lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchangesJ Sartorius
Institut für Genetik der Universität zu Köln, FRG
EMBO J 8:1265-70. 1989..Mutations of residues 1 and 2 may be combined with a mutation of residue 6. The resulting mutant protein binds specifically to an operator variant with three symmetric exchanges in base pairs 4, 5 and 6...
- Mutants that make more lac repressorB Müller-Hill
Proc Natl Acad Sci U S A 59:1259-64. 1968
- Crystal structure of the lactose operon repressor and its complexes with DNA and inducerM Lewis
Johnson Research Foundation, University of Pennsylvania, Philadelphia 19104, USA
Science 271:1247-54. 1996..Its key component is the lac repressor, a product of the lacI gene...
- The function of auxiliary operatorsB Müller-Hill
Institut fur Genetik, Universitat zu Koln, Germany
Mol Microbiol 29:13-8. 1998..For this purpose, a strategy is used over and over again. It is called increase in local concentration. How Escherichia coli uses this strategy efficiently is discussed...
- Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domainCharalampos G Kalodimos
Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, NL 3584 CH Utrecht, The Netherlands
EMBO J 21:2866-76. 2002....
- Feedback regulation in the lactose operon: a mathematical modeling study and comparison with experimental dataNecmettin Yildirim
Centre for Nonlinear Dynamics, McGill University, Montreal, Quebec, Canada H4X 2C1
Biophys J 84:2841-51. 2003....
- Multistability in the lactose utilization network of Escherichia coliErtugrul M Ozbudak
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nature 427:737-40. 2004..The phase diagram thus serves as a sensitive probe of molecular interactions and as a powerful tool for rational network design...
- Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressorRoberto Kopke Salinas
Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands
Chembiochem 6:1628-37. 2005....
- A bottom-up approach to gene regulationNicholas J Guido
Department of Biomedical Engineering, Bioinformatics Program, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
Nature 439:856-60. 2006..This work shows that the properties of regulatory subsystems can be used to predict the behaviour of larger, more complex regulatory networks, and that this bottom-up approach can provide insights into gene regulation...
- In silico evolved lac operons exhibit bistability for artificial inducers, but not for lactoseM J A van Hoek
Theoretical Biology Bioinformatics Group, Utrecht University, Utrecht, The Netherlands
Biophys J 91:2833-43. 2006..Nevertheless, for engineering purposes, this operon can be used as a bistable switch with artificial inducers...
- The effect of stochasticity on the lac operon: an evolutionary perspectiveMilan van Hoek
Theoretical Biology Bioinformatics Group, Utrecht University, Utrecht, The Netherlands
PLoS Comput Biol 3:e111. 2007..Moreover, we demonstrate that in silico evolution in a quantitative model, by mutating the parameters of interest, is a promising way to unravel the functional properties of biological systems...
- Bistable behavior in a model of the lac operon in Escherichia coli with variable growth rateM Santillan
Unidad Monterrey, Centro de Investigacion y Estudios Avanzados del IPN, Monterrey, Mexico
Biophys J 94:2065-81. 2008..The results are discussed in regard to the bistable behavior of the lactose operon. Special attention is paid to the effect that a variable growth rate has on the system dynamics...
- Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexesJulija Romanuka
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands
J Mol Biol 390:478-89. 2009....
- SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operonT Abo
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
EMBO J 19:3762-9. 2000..COLI: cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation...
- Modeling network dynamics: the lac operon, a case studyJose M G Vilar
The Rockefeller University, 1230 York Avenue, Box 34, New York, NY 10021, USA
J Cell Biol 161:471-6. 2003..We integrate three different levels of description (molecular, cellular, and that of cell population) into a single model, which seems to capture many experimental aspects of the system...
- Modeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformationDavid Swigon
Departments of Chemistry and Chemical Biology, Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA
Proc Natl Acad Sci U S A 103:9879-84. 2006....
- The mechanism of CAP-lac repressor binding cooperativity at the E. coli lactose promoterK M Vossen
Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey 17033, USA
J Mol Biol 255:44-54. 1996..reduced by the removal of DNA located upstream of the CAP binding site or by substitution of the dimeric lacI-18 mutant repressor for the wild-type tetrameric protein...
- 5'-Terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sitesD A Steege
Proc Natl Acad Sci U S A 74:4163-7. 1977..Third, the formation of stable secondary structures predicted for the untranslated I mRNA beyond chain-terminating nonsense mutations may prevent ribosome access to some potential reinitiation sites...
- A model of the lac repressor-operator complex based on physical and genetic dataB Kisters-Woike
Institut fur Genetik, Universitat zu Koln, Federal Republic of Germany
Eur J Biochem 198:411-9. 1991....
- Cloning and characterization of several dominant-negative and tight-binding mutants of lac repressorJ L Betz
Gene 42:283-92. 1986Using both general recombination and molecular cloning techniques, 13I-, I-d and Itb missense mutations in the lacI gene were transferred from F'lacIq episomes to ColE1 derivative plasmids...
- Catabolite repression of the lac operon. The contribution f trascriptional repressionM D Yudkin
Biochem J 114:307-11. 1969..It is also concluded that the extent of translational repression varies between strains...
- The nature of lactose operator constitive mutationsT F Smith
J Mol Biol 59:273-305. 1971
- Escherichia coli lac repressor-lac operator interaction and the influence of allosteric effectorsN Horton
Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA
J Mol Biol 265:1-7. 1997..Conversely, the anti-inducer orthonitrophenylfucoside (ONPF) which stabilizes the lac repressor-lac operator complex increases the binding affinity, particularly to the right half of the operator...
- Toggles and oscillators: new genetic circuit designsE M Judd
Department of Applied Physics, Stanford University
Bioessays 22:507-9. 2000..Further study of the observed behaviors not predicted by the model would lead to new insight into the properties of genetic networks. BioEssays 22:507-509, 2000...
- Structure and flexibility adaptation in nonspecific and specific protein-DNA complexesCharalampos G Kalodimos
Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands
Science 305:386-9. 2004..The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site...
- Single-molecule spectroscopic determination of lac repressor-DNA loop conformationMichael A Morgan
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA
Biophys J 89:2588-96. 2005The Escherichia coli lactose repressor protein (LacI) provides a classic model for understanding protein-induced DNA looping. LacI has a C-terminal four-helix bundle tetramerization domain that may act as a flexible hinge...
- Structure and function of negative feedback loops at the interface of genetic and metabolic networksSandeep Krishna
Center for Models of Life, Niels Bohr Institute, Blegdamsvej 17, 2100 Copenhagen, Denmark
Nucleic Acids Res 34:2455-62. 2006..We use three examples, the bet, mer and lac systems in Escherichia coli, to illustrate the behaviour of such feedback loops...
- The lac repressor displays facilitated diffusion in living cellsPetter Hammar
Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
Science 336:1595-8. 2012..Furthermore, the repressor frequently (>90%) slides over its natural lacO(1) operator several times before binding. This suggests a trade-off between rapid search on nonspecific sequences and fast binding at the specific sequence...
- The interaction of the recognition helix of lac repressor with lac operatorN Lehming
Institut für Genetik der Universität zu Köln, FRG
EMBO J 6:3145-53. 1987..The other plasmid carries the gene coding for the repressor, in our case a semisynthetic lacI gene of which parts can be exchanged in a cassette-like manner...
- Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activityT Platt
J Biol Chem 248:110-21. 1973
- Mutational inversion of control of the lactose operon of Escherichia coliG L Myers
J Mol Biol 58:1-28. 1971
- Genetic control of catabolite repression of the lac operon in Escherichia coliW F Loomis
Biochem Biophys Res Commun 20:230-4. 1965
- Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80J R Beckwith
J Mol Biol 19:254-65. 1966
- Fine-tuning function: correlation of hinge domain interactions with functional distinctions between LacI and PurRLiskin Swint-Kruse
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
Protein Sci 11:778-94. 2002b>LacI and PurR are highly homologous proteins. Their functional units are homodimers, with an N-terminal DNA binding domain that comprises the helix-turn-helix (HTH), N-linker, and hinge regions from both monomers...
- RNA polymerase structure and function at lac operonSergei Borukhov
Department of Cell Biology, UMDNJ SOM at Stratford, Stratford, NJ 08084, USA
C R Biol 328:576-87. 2005..by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ..
- What is life? The paradigm of DNA and protein cooperation at high local concentrationsBenno Müller-Hill
Institute of Genetics, University of Cologne, Zülpicher Str 47, D 50674 Cologne, Germany
Mol Microbiol 60:253-5. 2006..The examples of Lac and Lambda repressors of Escherichia coli are presented and discussed here as useful paradigms of mechanisms for achieving high local concentrations of interacting protein protomers...
- Predicting stochastic gene expression dynamics in single cellsJerome T Mettetal
Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 103:7304-9. 2006..Our results provide a proof of principle for the possibility of faithfully predicting dynamic population distributions from deterministic models supplemented by a stochastic component that captures the major noise sources...
- Mutational spectrum of the lacI gene in Escherichia coli K12 induced by low-energy ion beamMing Li Tang
Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, PR China
Mutat Res 602:163-9. 2006The mutational spectrum of the genomic lacI gene induced by low-energy nitrogen ion irradiation in wild type Escherichia coli strain W3110 were compared with the spontaneous and the vacuum controls...
- The diffusive influx and carrier efflux have a strong effect on the bistability of the lac operon in Escherichia coliJason T Noel
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611 6005, USA
J Theor Biol 256:14-28. 2009..3) The simulations are in good agreement with the data for isopropyl thiogalactoside (IPTG), but somewhat discrepant with respect to the data for thiomethyl galactoside (TMG). We discuss the potential sources of the discrepancy...
- Radiation damage to a DNA-binding protein. Combined circular dichroism and molecular dynamics simulation analysisS Mazier
Centre de Biophysique Moleculaire, CNRS, Orleans, France
Radiat Res 170:604-12. 2008..This conclusion should hold for all proteins containing radiosensitive amino acids in their DNA-binding site...
- Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectorsMartin Kawe
Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Microb Cell Fact 8:8. 2009..Because deletion is so facile, it might impact on high-throughput protein production, e.g. for structural genomics, where not every expression parameter will be monitored...
- Mimicking the evolution of a thermally stable monomeric four-helix bundle by fusion of four identical single-helix peptidesSatoshi Akanuma
Department of Molecular Biology, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
J Biochem 147:371-9. 2010..Moreover, our thermally stable single-chain four-helix bundle protein may provide a robust scaffold for development of new biomaterials...
- Exploring the sequence-function relationship in transcriptional regulation by the lac O1 operatorTuhin S Maity
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
FEBS J 279:2534-43. 2012..We investigated the behavior of the Escherichia coli Lac repressor (LacI) protein with a large set of lac O(1) operator variants. The 114 variants examined contained a mean of 2...
- Pseudoreversion of lactose operator-constitutive mutantsW I Norwood
J Bacteriol 130:100-6. 1977....
- Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase systemM H Saier
J Bacteriol 133:1358-67. 1978....
- Analysis of the regulatory mechanisms controlling the synthesis of the hexitol transport systems in Escherichia coli K12J Lengeler
Mol Gen Genet 164:163-9. 1978..The influence of these regulatory mechanisms in diauxie experiments and their importance for the differentiation of the three operons during evolution from apparently one common ancestor operon will be discussed...
- Mutant lac repressors with new specificities hint at rules for protein--DNA recognitionN Lehming
Institut fur Genetik, Universitat zu Koln, FRG
EMBO J 9:615-21. 1990..Rules of recognition can be derived for 16 symmetric operators. They also apply to the gal repressor and possibly to other bacterial repressors...
- Missense mutation in the lacI gene of Escherichia coli. Inferences on the structure of the repressor proteinA J Gordon
Biology Department, York University, Toronto, Ontario, Canada
J Mol Biol 200:239-51. 1988..We have been using the lacI gene-repressor protein system to study the mutational specificity of spontaneous and induced mutation...
- How lac repressor binds to DNAK Adler
Nature 237:322-7. 1972
- Mutations partially inactivating the lactose repressor of Escherichia coliB Shineberg
J Bacteriol 119:500-7. 1974..An explanation is proposed for the apparent sensitivity of this repressor function to partial inactivation as the result of amino acid substitutions...
- Sequential transcription and translation in the lactose operon of Escherichia coliA Kepes
Biochim Biophys Acta 138:107-23. 1967
- Relationships between the regulation of the lactose and galactose operons of Escherichia coliB Williams
J Bacteriol 97:769-75. 1969..For this operon, the differential rate of enzyme synthesis is set by the relative intracellular concentrations of inducer (fucose) and repressor (isopropylthiogalactoside)...
- Re-initiation of polypeptide synthesis and polarity in the lac operon of Escherichia coliA Newton
J Mol Biol 41:329-39. 1969
- Synthesis, utilization and degradation of lactose operon mRNA in Escherichia coliL Leive
J Mol Biol 24:247-59. 1967
- Lac repressorB Müller-Hill
Angew Chem Int Ed Engl 10:160-72. 1971
- Sequential transcription of the genes of the lactose operon and its regulation by protein synthesisD H Alpers
J Biol Chem 241:4434-43. 1966
- Regulation of the lac operon. Recent studies on the regulation of lactose metabolism in Escherichia coli support the operon modelJ R Beckwith
Science 156:597-604. 1967
- Synthesis of enzymes of the lactose operon during diauxic growth of Escherichia coliW Epstein
Biochem Biophys Res Commun 24:588-92. 1966
- A mutant Ebg enzyme that converts lactose into an inducer of the lac operonS J Rolseth
J Bacteriol 142:1036-9. 1980..We report here the isolation of a mutant Ebg beta-galactosidase which is capable of converting lactose into an inducer of the lac operon...
- Transgalactosylation activity of ebg beta-galactosidase synthesizes allolactose from lactoseB G Hall
J Bacteriol 150:132-40. 1982..The evolutionary implications of this new function are discussed...
- Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequenceP Markiewicz
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024
J Mol Biol 240:421-33. 1994..have been used to insert, in series, 12-13 amino acids at positions specified by an amber (UAG) codon in the lacI mRNA...
- Backbone and side chain dynamics of lac repressor headpiece (1-56) and its complex with DNAM Slijper
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands
Biochemistry 36:249-54. 1997..These results suggest that the mobility of the regions within lac HP56 important for complexation, i.e., the loop and the DNA-contacting side chains, is essential for a good fit onto the counterparts of the target DNA...
- Hinge-helix formation and DNA bending in various lac repressor-operator complexesC A Spronk
Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
EMBO J 18:6472-80. 1999....
- Structure of a variant of lac repressor with increased thermostability and decreased affinity for operatorC E Bell
The Johnson Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, PA 19102 6059, USA
J Mol Biol 313:99-109. 2001....
- Perturbation from a distance: mutations that alter LacI function through long-range effectsLiskin Swint-Kruse
W M Keck Center for Computational Biology, Rice University, 6100 South Main Street, Houston, Texas 77005, USA
Biochemistry 42:14004-16. 2003Allosteric modification of ligand binding is central to LacI transcription control. Recently, the conformational change between LacI operator- and inducer-bound states was simulated with targeted molecular dynamics (TMD) [Flynn, T. C...
- Structural basis for cooperative DNA binding by CAP and lac repressorAlexander Balaeff
Center for Biophysics and Computational Biology and Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Structure 12:123-32. 2004..Using the multiscale approach, we build an all-atom model of the ternary complex that suggests a series of further experimental investigations...
- Specificity of spontaneous mutations induced in mutA mutator cellsSergey Balashov
Department of Microbiology and Molecular Genetics, International Center for Public Health, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, NJ 07101 1709, USA
Mutat Res 548:9-18. 2004..Here, we have determined the specificity of forward mutations occurring in the lacI gene in mutA cells as well as in wild type cells...
- Biochemistry. Completing the view of transcriptional regulationPeter H von Hippel
Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
Science 305:350-2. 2004
- Adaptive point mutation and adaptive amplification pathways in the Escherichia coli Lac system: stress responses producing genetic changeSusan M Rosenberg
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, BCM S809A Mail Stop BCM225, Houston, TX 77030 3411, USA
J Bacteriol 186:4838-43. 2004
- Rebuttal: adaptive point mutation (Rosenberg and Hastings)John R Roth
University of California Davis, Microbiology Section, One Shields Ave, Davis, CA 95616, USA
J Bacteriol 186:4844. 2004
- Activity of Lac repressor anchored to the Escherichia coli inner membraneBoris Görke
Institut fur Biologie III, Universität Freiburg Schänzlestrasse 1, D 79104 Freiburg, Germany
Nucleic Acids Res 33:2504-11. 2005..Other mechanisms, like induction of a conformational change or masking of binding domains are required additionally...
- Combinatorial transcriptional control of the lactose operon of Escherichia coliThomas Kuhlman
Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093 0374, USA
Proc Natl Acad Sci U S A 104:6043-8. 2007..Specifically, our analysis indicates that the sensitivity of the inducer response results from LacR-mediated DNA looping, which is significantly enhanced by CRP...
- Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium compositionPaul G Blommel
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Biotechnol Prog 23:585-98. 2007..coli is based on diauxic growth resulting from dynamic function of lac operon regulatory elements (lacO and LacI) in mixtures of glucose, glycerol, and lactose...
- Probing transcription factor dynamics at the single-molecule level in a living cellJohan Elf
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 316:1191-4. 2007..The methods and findings can be generalized to other nucleic acid binding proteins...
- Integration host factor alters LacI-induced DNA loopingChiara Zurla
Department of Physics, Emory University, Atlanta, GA 30322, USA
Biophys Chem 128:245-52. 2007..coli. In single molecule experiments, we show that both specific bending and non-specific compaction alter LacI-mediated looping of DNA...
- Effects of nucleoid proteins on DNA repression loop formation in Escherichia coliNicole A Becker
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905, USA
Nucleic Acids Res 35:3988-4000. 2007..These results suggest that host nucleoid proteins can directly facilitate or inhibit DNA looping in bacteria...
- Dynamic properties of a delayed protein cross talk modelSvetoslav Nikolov
Institute of Mechanics and Biomechanics BAS, Acad G Bonchev Str, Bl 4, 1113 Sofia, Bulgaria
Biosystems 91:51-68. 2008....
- Modelling gene expression control using P systems: The Lac Operon, a case studyFrancisco Jose Romero-Campero
Research Group on Natural Computing, Department of Computer Science and Artificial Intelligence, University of Sevilla, Avda Reina Mercedes s n, 41012 Sevilla, Spain
Biosystems 91:438-57. 2008..The well known gene regulation system in the Lac Operon in Escherichia coli will be modelled as a case study to benchmark our approach...
- Substitutions at auxiliary operator O3 enhance repression by nitrate-responsive regulator NarL at synthetic lac control regions in Escherichia coli K-12Valley Stewart
Section of Microbiology, University of California, One Shields Avenue, Davis CA 95616 8665, USA
J Bacteriol 190:428-33. 2008..The results support the hypothesis that DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interactions...
- Stochastic simulations of the repressilator circuitAdiel Loinger
Racah Institute of Physics, The Hebrew University, Jerusalem 91904, Israel
Phys Rev E Stat Nonlin Soft Matter Phys 76:051917. 2007..We also find that subtle features such as cooperative binding and bound-repressor degradation strongly affect the existence and properties of the oscillations...
- Quantitative approaches to the study of bistability in the lac operon of Escherichia coliMoises Santillan
Centro de Investigacion y Estudios Avanzados del IPN, Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Apodaca, NL, Mexico
J R Soc Interface 5:S29-39. 2008..A review of the literature in which this bistable phenomenon has been studied from a mathematical modelling viewpoint is then given. We conclude with some brief remarks...
- Allostery in the LacI/GalR family: variations on a themeLiskin Swint-Kruse
Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, 66160, United States
Curr Opin Microbiol 12:129-37. 2009The lactose repressor protein (LacI) was among the very first genetic regulatory proteins discovered, and more than 1000 members of the bacterial LacI/GalR family are now identified...
- Flexibility in the inducer binding region is crucial for allostery in the Escherichia coli lactose repressorJia Xu
Department of Biochemistry and Cell Biology, Rice University, 6100 South Main Street, Houston, Texas 77005, USA
Biochemistry 48:4988-98. 2009Lactose repressor protein (LacI) utilizes an allosteric mechanism to regulate transcription in Escherichia coli, and the transition between inducer- and operator-bound states has been simulated by targeted molecular dynamics (TMD)...
- Radiation-induced tetramer-to-dimer transition of Escherichia coli lactose repressorS Goffinont
Centre de Biophysique Moleculaire, CNRS, Orleans, France
Biochem Biophys Res Commun 386:300-4. 2009..The splitting of the tetramer into dimers can result from the oxidation of the leucine residues of the tetramerization domain...
- Adaptive prediction of environmental changes by microorganismsAmir Mitchell
Department of Molecular Genetics, Weizmann Institute of Science Rehovot 76100, Israel
Nature 460:220-4. 2009..Our work indicates that environmental anticipation is an adaptive trait that was repeatedly selected for during evolution and thus may be ubiquitous in biology...
- First-principles calculation of DNA looping in tethered particle experimentsKevin B Towles
Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA
Phys Biol 6:025001. 2009We calculate the probability of DNA loop formation mediated by regulatory proteins such as Lac repressor (LacI), using a mathematical model of DNA elasticity...
- High local concentration: a fundamental strategy of lifeStefan Oehler
Biomedical Sciences Research Center Alexander Fleming, 34 Fleming Street, GR 16672 Vari, Greece
J Mol Biol 395:242-53. 2010..Local increase in concentration leads to cooperative or competitive interactions between molecules. It is an important principle of life...
- A frameshift mutation at the junction of an IS1 insertion within lacZ restores beta-galactosidase activity via formation of an active lacZ-IS1 fusion proteinM H Malamy
J Mol Biol 181:551-5. 1985..The IS1 element in MS319 is a new member of the iso-IS1 family, which we designate IS1T...
- lac Up-promoter mutants with increased homology to the consensus promoter sequenceW Mandecki
J Bacteriol 164:1353-5. 1985..All mutants exhibited higher activity than did the wild-type promoter...
- Effects of heterologous ribosomal binding sites on the transcription and translation of the lacZ gene of Escherichia coliA C Looman
Gene 37:145-54. 1985..No strict correlation could be found between the level of lac mRNA and beta-gal production. Per molecule of lac mRNA, translation appears to be most efficient when the homologous lacZ initiation signal is present...
- Genetic and sequencing studies of the specificity of transposition into the lac region of E. coliJ H Miller
Cold Spring Harb Symp Quant Biol 45:243-57. 1981
- Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacingD R Russell
Gene 20:231-43. 1982
- Footprinting with MPE.Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNAM W Van Dyke
Cold Spring Harb Symp Quant Biol 47:347-53. 1983
- A perfectly symmetric lac operator binds the lac repressor very tightlyJ R Sadler
Proc Natl Acad Sci U S A 80:6785-9. 1983..The natural operator is apparently "flawed" by "incorrect" base pairs in the right operator half and by an "incorrect" spacing between the operator halves with respect to maximal repressor binding...
- Variants of a cloned synthetic lactose operator. I. A palindromic dimer lactose operator derived from one stand of the cloned 40-base pair operatorJ L Betz
Gene 13:1-12. 1981..Each of the conformations of this DNA fragment have been purified and all are active in binding lactose repressor in vitro...
- MOLECULAR INTERACTION IN T4 DNA REPLICATION COMPLEXPETER VON HIPPEL; Fiscal Year: 2003..abstract_text> ..
- Structure & Function of Prokaryotic Transcript CleavageSergei Borukhov; Fiscal Year: 2005..abstract_text> ..
- Molecular mechanism of DNA-polymerase choice in vivoSusan Rosenberg; Fiscal Year: 2007..coli DNA polymerases, and functions in DNA polymerase choice in vivo: a critical regulatory decision for cell survival of environmental damage, and genomic health. [unreadable] [unreadable] [unreadable]..
- DNA REPAIR IN DRUG RESISTANCE MUTATIONSusan Rosenberg; Fiscal Year: 2003....
- BIOSYNTHESIS OF VITAMIN B12 AND ANAEROBIC METABOLISMJohn Roth; Fiscal Year: 2004..coli. Understanding this metabolism may contribute to understanding the natural lifestyle of Salmonellae. ..
- Mechanisms of Adaptive AmplificationPhilip J Hastings; Fiscal Year: 2010....
- GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLIValley J Stewart; Fiscal Year: 2010..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..