minD

Summary

Gene Symbol: minD
Description: inhibitor of FtsZ ring polymerization; chromosome-membrane tethering protein; membrane ATPase of the MinCDEE system
Alias: ECK1163, JW1164, minB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Cell division in Escherichia coli minB mutants
    T Akerlund
    Department of Microbiology, Uppsala University, Sweden
    Mol Microbiol 6:2073-83. 1992
  2. pmc The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site
    P A de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06030
    EMBO J 10:4371-80. 1991
  3. ncbi A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli
    P A de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06032
    Cell 56:641-9. 1989
  4. ncbi The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli
    D M Raskin
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    Cell 91:685-94. 1997
  5. pmc Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site
    A L Marston
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Genes Dev 12:3419-30. 1998
  6. pmc Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
    D M Raskin
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4960, USA
    Proc Natl Acad Sci U S A 96:4971-6. 1999
  7. ncbi Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
    Z Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 34:82-90. 1999
  8. pmc Membrane redistribution of the Escherichia coli MinD protein induced by MinE
    S L Rowland
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 182:613-9. 2000
  9. pmc The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle
    X Fu
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 98:980-5. 2001
  10. pmc Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus
    I Hayashi
    Department of Structural Biology, Biomolecular Engineering Research Institute, 6 2 3 Furuedai, Suita City, Osaka 565 0874, Japan
    EMBO J 20:1819-28. 2001

Research Grants

  1. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
  2. BACTERIAL CELL DIVISION
    Lawrence Rothfield; Fiscal Year: 2002
  3. E COLI MIN PROTEINS
    Lawrence Rothfield; Fiscal Year: 2003

Scientific Experts

Detail Information

Publications104 found, 100 shown here

  1. ncbi Cell division in Escherichia coli minB mutants
    T Akerlund
    Department of Microbiology, Uppsala University, Sweden
    Mol Microbiol 6:2073-83. 1992
    In Escherichia coli minB mutants, cell division can take place at the cell poles as well as non-polarly in the cell...
  2. pmc The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site
    P A de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06030
    EMBO J 10:4371-80. 1991
    ..site-specific inactivation of potential division sites at the cell poles in a process that is mediated by the MinC, MinD and MinE proteins. During the normal division cycle MinD plays two roles...
  3. ncbi A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli
    P A de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06032
    Cell 56:641-9. 1989
    The E. coli minicell locus (minB) was shown to code for three gene products (MinC, MinD, and MinE) whose coordinate action is required for proper placement of the division spetum...
  4. ncbi The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli
    D M Raskin
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    Cell 91:685-94. 1997
    ..Formation of the MinE ring required MinD but was independent of MinC and continued in nondividing cells in which FtsZ function was inhibited...
  5. pmc Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site
    A L Marston
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Genes Dev 12:3419-30. 1998
    ..DivIVA then recruits MinD to the division sites preventing another division from taking place near the newly formed cell poles...
  6. pmc Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
    D M Raskin
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4960, USA
    Proc Natl Acad Sci U S A 96:4971-6. 1999
    ..of a general division inhibitor (MinC), a site-specific suppressor of division inhibition (MinE), and an ATPase (MinD) that is required for proper functioning of both MinC and MinE...
  7. ncbi Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
    Z Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 34:82-90. 1999
    ..This activity of min is achieved through the action of an inhibitor of division, MinC, that is activated by MinD and topologically regulated by MinE...
  8. pmc Membrane redistribution of the Escherichia coli MinD protein induced by MinE
    S L Rowland
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 182:613-9. 2000
    ..Selection of the correct division site at midcell is controlled by three proteins: MinC, MinD, and MinE. It has previously been shown (D. Raskin and P...
  9. pmc The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle
    X Fu
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 98:980-5. 2001
    ..Taken together with studies of the dynamic behavior of the MinD protein, the results suggest that the topological specificity of division site placement may not involve a ..
  10. pmc Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus
    I Hayashi
    Department of Structural Biology, Biomolecular Engineering Research Institute, 6 2 3 Furuedai, Suita City, Osaka 565 0874, Japan
    EMBO J 20:1819-28. 2001
    ..In Escherichia coli, selection of the correct mid-cell site is mediated by the MinC, MinD and MinE proteins...
  11. ncbi Topological regulation of cell division in E. coli. spatiotemporal oscillation of MinD requires stimulation of its ATPase by MinE and phospholipid
    Z Hu
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 66160, Kansas City, KS, USA
    Mol Cell 7:1337-43. 2001
    ..This positioning is achieved through a rapid oscillation of MinC from pole to pole, a process requiring MinD and MinE. However, the mechanistic basis for this oscillation is not known...
  12. pmc Pattern formation in Escherichia coli: a model for the pole-to-pole oscillations of Min proteins and the localization of the division site
    H Meinhardt
    Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 98:14202-7. 2001
    ..we show that Min protein dynamics can be described accurately by using the following assumptions: (i) the MinD ATPase self-assembles on the membrane and recruits both MinC, an inhibitor of Z ring formation, and MinE, a protein ..
  13. ncbi Dynamic compartmentalization of bacteria: accurate division in E. coli
    M Howard
    Department of Physics, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Phys Rev Lett 87:278102. 2001
    ..coli is controlled by the min system of proteins: MinC, MinD, and MinE. These proteins coherently oscillate from end to end of the bacterium...
  14. pmc A dynamic model for determining the middle of Escherichia coli
    Karsten Kruse
    Max Planck Institut für Strömungsforschung, D 37073 Gottingen, Germany
    Biophys J 82:618-27. 2002
    ..In Escherichia coli, this process depends crucially on the proteins MinC, MinD, and MinE...
  15. pmc Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE
    Zonglin Hu
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 99:6761-6. 2002
    ..of the division site in Escherichia coli is regulated by the min system and requires the rapid oscillation of MinD between the two halves of the cell under the control of MinE...
  16. pmc Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains
    Yu Ling Shih
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032, USA
    EMBO J 21:3347-57. 2002
    ..MinE assembles into a membrane-associated ring structure near midcell and directs the localization of MinD and MinC into a membrane- associated polar zone that undergoes a characteristic pole-to-pole oscillation cycle...
  17. ncbi Effects of the Min system on nucleoid segregation in Escherichia coli
    Thomas Akerlund
    Department of Bacteriology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden
    Microbiology 148:3213-22. 2002
    ..the mid-cell by a mechanism that involves the dynamic localization of all of its three constituent proteins, MinC, MinD and MinE. Both the Min system and the nucleoid regulate cell division negatively and strains of E...
  18. ncbi Conservation of dynamic localization among MinD and MinE orthologues: oscillation of Neisseria gonorrhoeae proteins in Escherichia coli
    Sandra Ramirez-Arcos
    Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada
    Mol Microbiol 46:493-504. 2002
    ..coli. Green fluorescent protein (GFP) fusions to gonococcal MinD and MinE localized dynamically in different E. coli backgrounds. GFP-MinDNg moved from pole to pole in rod-shaped E...
  19. pmc Membrane localization of MinD is mediated by a C-terminal motif that is conserved across eubacteria, archaea, and chloroplasts
    Tim H Szeto
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, 06032, USA
    Proc Natl Acad Sci U S A 99:15693-8. 2002
    b>MinD is a widely conserved ATPase that has been demonstrated to play a pivotal role in selection of the division site in eubacteria and chloroplasts...
  20. pmc Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE
    Kyoko Suefuji
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 99:16776-81. 2002
    Accurate positioning of the division septum at the equator of Escherichia coli cells requires a rapid oscillation of MinD ATPase between the polar halves of the cell membrane, together with the division inhibitor MinC, under MinE control...
  21. ncbi A conserved sequence at the C-terminus of MinD is required for binding to the membrane and targeting MinC to the septum
    Zonglin Hu
    Department of Microbiology, University of Kansas Medical Center, KS 66160, USA
    Mol Microbiol 47:345-55. 2003
    b>MinD is a key component of an oscillatory system that spatially regulates cell division in Escherichia coli...
  22. pmc ATP-dependent interactions between Escherichia coli Min proteins and the phospholipid membrane in vitro
    Laura L Lackner
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4960, USA
    J Bacteriol 185:735-49. 2003
    ..MinC dynamics involves a membrane association-dissociation cycle that is driven by the activities of the MinD ATPase and the MinE topological specificity factor, which themselves undergo coupled oscillatory localization ..
  23. ncbi Effects of phospholipid composition on MinD-membrane interactions in vitro and in vivo
    Eugenia Mileykovskaya
    Department of Biochemistry, The University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 278:22193-8. 2003
    The peripheral membrane ATPase MinD is a component of the Min system responsible for correct placement of the division site in Escherichia coli cells...
  24. ncbi Pattern formation inside bacteria: fluctuations due to the low copy number of proteins
    Martin Howard
    Instituut Lorentz, Leiden University, P O Box 9506, 2300 RA Leiden, The Netherlands
    Phys Rev Lett 90:128102. 2003
    ..We find that, for some parameter regions, the protein concentrations are low enough that fluctuations are essential for the generation of patterns. We also examine the role of fluctuations in constraining protein concentration levels...
  25. pmc Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles
    Yu Ling Shih
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Proc Natl Acad Sci U S A 100:7865-70. 2003
    ..coli and that structures of this type can undergo dynamic changes that play important roles in division site placement and possibly other aspects of the life of the cell...
  26. pmc Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160 7420, USA
    J Bacteriol 185:4326-35. 2003
    b>MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix...
  27. ncbi The MinD membrane targeting sequence is a transplantable lipid-binding helix
    Tim H Szeto
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Biol Chem 278:40050-6. 2003
    b>MinD is a ubiquitous ATPase that plays a crucial role in selection of the division site in eubacteria, chloroplasts, and probably also Archaea...
  28. pmc Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones
    Kerwyn Casey Huang
    Department of Physics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 12 111, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:12724-8. 2003
    ..oscillatory structures produced by the Min proteins, including a ring of MinE protein, compact polar zones of MinD, and zebra-striped oscillations in filamentous cells, remain unexplained...
  29. pmc FtsZ exhibits rapid movement and oscillation waves in helix-like patterns in Escherichia coli
    Swapna Thanedar
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA
    Curr Biol 14:1167-73. 2004
    ..The MreB helix was not required for the rapid movement of FtsZ or the oscillation of MinD. The results suggest that FtsZ not only forms the Z ring but also is part of a highly dynamic, potentially helical ..
  30. pmc Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins
    David E Anderson
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 186:5775-81. 2004
    ..In B. subtilis, only 30 to 35% of the FtsZ protein was in the Z ring, from which we conclude that a Z ring only 2 or 3 protofilaments thick can function for cell division...
  31. pmc Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 187:629-38. 2005
    The MinD ATPase is critical to the oscillation of the Min proteins, which limits formation of the Z ring to midcell...
  32. pmc A polymerization-depolymerization model that accurately generates the self-sustained oscillatory system involved in bacterial division site placement
    Donald A Drew
    Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180 3590, USA
    Proc Natl Acad Sci U S A 102:6114-8. 2005
    ..bacteria involves a unique spatial oscillatory system in which membrane-associated structures composed of the MinC, MinD and MinE proteins oscillate rapidly between the two cell poles...
  33. ncbi The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation
    Yu Ling Shih
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Mol Microbiol 58:917-28. 2005
    ..Thus, the two bacterial cytoskeletal-like systems act independently on different aspects of cell polarization...
  34. ncbi A stochastic model of Min oscillations in Escherichia coli and Min protein segregation during cell division
    Filipe Tostevin
    Department of Mathematics, Imperial College London, London SW7 2AZ, UK
    Phys Biol 3:1-12. 2005
    ..We present a one-dimensional stochastic model of these oscillations which incorporates membrane polymerization of MinD into linear chains...
  35. pmc Temperature dependence of MinD oscillation in Escherichia coli: running hot and fast
    Ahmed Touhami
    Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia B3H 3J5, Canada
    J Bacteriol 188:7661-7. 2006
    We observed that the oscillation period of MinD within rod-like and filamentous cells of Escherichia coli varied by a factor of 4 in the temperature range from 20 degrees C to 40 degrees C...
  36. pmc An experimentalist's guide to computational modelling of the Min system
    Karsten Kruse
    Max Planck Institut fur Physik komplexer Systeme, Nothnitzer Str 38, D 01187 Dresden, Germany
    Mol Microbiol 63:1279-84. 2007
    ..We also review the effects of fluctuations caused by low cellular concentration of Min proteins, and describe how stochastic effects may potentially influence Min protein dynamics...
  37. ncbi Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Annu Rev Biochem 76:539-62. 2007
    ..Additional gradients of negative regulators of FtsZ assembly are used by E. coli and other bacteria to achieve spatial control of Z-ring assembly...
  38. pmc Reconstitution of contractile FtsZ rings in liposomes
    Masaki Osawa
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 3709, USA
    Science 320:792-4. 2008
    ..Brighter Z rings produced visible constrictions in the liposome, suggesting that FtsZ itself can assemble the Z ring and generate a force. No other proteins were needed for assembly and force generation...
  39. doi Spatial regulators for bacterial cell division self-organize into surface waves in vitro
    Martin Loose
    Biotechnologisches Zentrum der Technischen Universität Dresden, Tatzberg 47 51, 01307 Dresden, Germany
    Science 320:789-92. 2008
    ..We present a reaction-diffusion model of the MinD and MinE dynamics that accounts for our experimental observations and also captures the in vivo oscillations.
  40. pmc Changes in the Min oscillation pattern before and after cell birth
    Jennifer R Juarez
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, TX 77030, USA
    J Bacteriol 192:4134-42. 2010
    The Min system regulates the positioning of the cell division site in many bacteria. In Escherichia coli, MinD migrates rapidly from one cell pole to the other...
  41. pmc Determination of the structure of the MinD-ATP complex reveals the orientation of MinD on the membrane and the relative location of the binding sites for MinE and MinC
    Wei Wu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 79:1515-28. 2011
    ..b>MinD binds to vesicles in the presence of ATP and can recruit MinC or MinE...
  42. pmc MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli
    D M Raskin
    Department of Molecular Biology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106 4960, USA
    J Bacteriol 181:6419-24. 1999
    ..MinC activity requires that of MinD, and the MinE peptide provides topological specificity by suppressing MinC-MinD-mediated division inhibition ..
  43. pmc Dynamic localization cycle of the cell division regulator MinE in Escherichia coli
    C A Hale
    Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4960, USA
    EMBO J 20:1563-72. 2001
    ..b>MinD and MinE regulate MinC activity by modulating its cellular location in a unique fashion...
  44. pmc Exploring intracellular space: function of the Min system in round-shaped Escherichia coli
    Brian D Corbin
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    EMBO J 21:1998-2008. 2002
    ..In round cells with a long axis, such as those undergoing cell division, green fluorescent protein (GFP) fusions to MinD almost always oscillated parallel to the long axis...
  45. pmc Division accuracy in a stochastic model of Min oscillations in Escherichia coli
    Rex A Kerr
    Computational Neurobiology Laboratory and Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 103:347-52. 2006
    Accurate cell division in Escherichia coli requires the Min proteins MinC, MinD, and MinE as well as the presence of nucleoids...
  46. pmc A factor that positively regulates cell division by activating transcription of the major cluster of essential cell division genes of Escherichia coli
    X D Wang
    Department of Microbiology, University of Connecticut Health Center, Farmington 06032
    EMBO J 10:3363-72. 1991
    ..sdiA gene, which was isolated on the basis of its ability to suppress the division inhibitory effect of the MinC/MinD division inhibitor...
  47. pmc Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems
    P A de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06032
    Proc Natl Acad Sci U S A 87:1129-33. 1990
    ..septation is mediated by the gene products of the minicell locus (the minB operon) that includes three genes, minC, minD, and minE...
  48. pmc The switch I and II regions of MinD are required for binding and activating MinC
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 186:1546-55. 2004
    b>MinD and MinC cooperate to form an efficient inhibitor of Z-ring formation that is spatially regulated by MinE. MinD activates MinC by recruiting it to the membrane and targeting it to a septal component...
  49. pmc Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli
    Felipe O Bendezú
    Case Western Reserve University, School of Medicine, Department of Molecular Biology and Microbiology, Cleveland, OH 44106, USA
    J Bacteriol 190:1792-811. 2008
    ..Both FtsZ and MinD readily assembled on intracytoplasmic membrane surfaces, and we propose that this contributes significantly to the ..
  50. pmc Subcellular Min oscillations as a single-cell reporter of the action of polycations, protamine, and gentamicin on Escherichia coli
    Benjamin P B Downing
    Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia, Canada
    PLoS ONE 4:e7285. 2009
    In Escherichia coli, MinD-GFP fusion proteins show rapid pole to pole oscillations. The objective was to investigate the effects of extracellular cations on the subcellular oscillation of cytoplasmic MinD within Escherichia coli.
  51. pmc MinCD cell division proteins form alternating copolymeric cytomotive filaments
    Debnath Ghosal
    Structural Studies, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK
    Nat Commun 5:5341. 2014
    ..Its positioning is regulated by the oscillation of MinCDE proteins. MinC is activated by MinD through an unknown mechanism and prevents Z-ring assembly anywhere but midcell...
  52. pmc MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli
    Lars D Renner
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 287:38835-44. 2012
    The Min proteins (MinC, MinD, and MinE) form a pole-to-pole oscillator that controls the spatial assembly of the division machinery in Escherichia coli cells...
  53. pmc Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods
    A Jaffe
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Paris, France
    J Bacteriol 170:3094-101. 1988
    The Escherichia coli minB mutant originally isolated is known to septate at cell poles to form spherical anucleate minicells...
  54. pmc Genetic basis of minicell formation in Escherichia coli K-12
    E Davie
    J Bacteriol 158:1202-3. 1984
    ..experiments failed to confirm the presence of a " minA " gene in Escherichia coli K-12, leading to the conclusion that mutation at a single locus, the minB locus, is sufficient to cause minicell production in this species.
  55. pmc The min locus, which confers topological specificity to cell division, is not involved in its coupling with nucleoid separation
    M Dassain
    Laboratoire de Microbiologie et de Genetique Moleculaire du Centre National de la Recherche Scientifique, Toulouse, France
    J Bacteriol 176:6143-5. 1994
    ..We suggest that the retarded nucleoid segregation observed in min mutants is the result of this coupling in cells with a perturbed pattern of nonpolar divisions...
  56. ncbi Branched Escherichia coli cells
    T Akerlund
    Department of Microbiology, Uppsala University, Sweden
    Mol Microbiol 10:849-58. 1993
    ..These were found in strains in which chromosome replication or nucleoid segregation was disturbed, e.g. in minB mutants, intR1 strains, and in strains exhibiting stable DNA replication...
  57. ncbi The minCD locus of Bacillus subtilis lacks the minE determinant that provides topological specificity to cell division
    S Lee
    Department of Food Science and Technology, University of California, Davis 95616
    Mol Microbiol 7:601-10. 1993
    ..subtilis counterpart to the Escherichia coli minicell operon minB, which governs correct placement of the division septum...
  58. pmc MinCD-independent inhibition of cell division by a protein that fuses MalE to the topological specificity factor MinE
    S Pichoff
    Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Toulouse, France
    J Bacteriol 179:4616-9. 1997
    ..We also report that high-level synthesis of MalE disturbs nucleoid partitioning...
  59. ncbi FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization
    X C Yu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 32:315-26. 1999
    ..A model is proposed in which both the inhibitory effect of the nucleoid and the regulation by MinCDE ensure that cells divide precisely at the midpoint...
  60. ncbi Bacterial division: Finding the dividing line
    S M Sullivan
    Department of Biology, University of Michigan, Ann Arbor 48109, USA
    Curr Biol 10:R249-52. 2000
    ..Recent work on the bacterium Escherichia coli has shown that correct placement of the cell division site at the midcell position occurs by a combination of selection against potential polar sites and selection of the midcell site...
  61. ncbi A chromosomal locus encoding a phosphoserine phosphatase- and a truncated MinD-like protein affects differentiation in Streptomyces azureus ATCC14921
    T Nishiyama
    Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
    FEMS Microbiol Lett 190:133-9. 2000
    ..The deduced products were somewhat similar to phosphoserine phosphatase-like protein and the N-terminal region of MinD-like protein, respectively...
  62. ncbi FtsZ rings in mukB mutants with or without the Min system
    X C Yu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston 77030, USA
    Biochimie 83:125-9. 2001
    ..These results provide further evidence that the negative topological effect of nucleoids in cells lacking MukB is partially but not totally suppressed, and that the absence of the Min system allows more promiscuous Z ring formation...
  63. ncbi The MinD protein from the hyperthermophilic archaeon Pyrococcus horikoshii: crystallization and preliminary X-ray analysis
    N Sakai
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    Acta Crystallogr D Biol Crystallogr 57:896-7. 2001
    b>MinD is one of the proteins regulating cell division. MinD from Escherichia coli has been designated as a type of motor protein which has an ATPase activity...
  64. ncbi Co-ordinate regulation of the Escherichia coli cell cycle or The cloud of unknowing
    W D Donachie
    Department of Molecular Biology, University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh, UK
    Mol Microbiol 40:779-85. 2001
    ..A discussion of some aspects of the regulation of chromosome replication, segregation and cell division in Escherichia coli...
  65. pmc Division site selection protein DivIVA of Bacillus subtilis has a second distinct function in chromosome segregation during sporulation
    H B Thomaides
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Genes Dev 15:1662-73. 2001
    ..DivIVA therefore appears to be a bifunctional protein with distinct roles in division-site selection and chromosome segregation...
  66. ncbi Polar explorers: membrane proteins that determine division site placement
    L I Rothfield
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Cell 106:13-6. 2001
  67. ncbi The bacterial ParA-ParB partitioning proteins
    C Bignell
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Biotechnol 91:1-34. 2001
    ..In Bacillus subtilis ParA oscillates from end to end of the cell as does MinD of E. coli, a relative of the ParA family...
  68. pmc Escherichia coli division inhibitor MinCD blocks septation by preventing Z-ring formation
    S Pichoff
    Department of Microbiology, University of Kansas Medical Center, Kansas City 66160, USA
    J Bacteriol 183:6630-5. 2001
    ..These results are consistent with FtsZ as the target of MinCD and confirm that this inhibitor prevents Z-ring assembly...
  69. ncbi Dynamic proteins in bacteria
    S Autret
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    Dev Cell 1:10-1. 2001
    The MinCDE system regulates the position of the division plane in rod-shaped bacteria. New results from Escherichia coli provide insight into how this operates by showing that MinE stimulates the ATPase activity of MinD.
  70. ncbi Spatial regulation of cytokinesis in bacteria
    W Margolin
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Curr Opin Microbiol 4:647-52. 2001
    ..This review summarizes and integrates these insights...
  71. ncbi Expression of Neisseria gonorrhoeae cell division genes ftsZ, ftsE and minD is influenced by environmental conditions
    S Ramirez-Arcos
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ontario, Canada
    Res Microbiol 152:781-91. 2001
    The activity of the promoter regions of the cell division genes ftsZ, ftsE, minC, minD and minE from Neisseria gonorrhoeae (Ng) was studied under different environmental conditions using lacZ translational fusions...
  72. pmc Targeting of (D)MinC/MinD and (D)MinC/DicB complexes to septal rings in Escherichia coli suggests a multistep mechanism for MinC-mediated destruction of nascent FtsZ rings
    Jay E Johnson
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4960, USA
    J Bacteriol 184:2951-62. 2002
    ..whereas the C-terminal domain ((D)MinC) is required for dimerization as well as for interaction with the MinD protein. MinD oscillates between the membrane of both cell halves in a MinE-dependent fashion...
  73. pmc Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli
    Lu Yan Ma
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:4948-55. 2003
    Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum...
  74. ncbi Pattern formation within Escherichia coli: diffusion, membrane attachment, and self-interaction of MinD molecules
    Rahul V Kulkarni
    NEC Laboratories America, Inc, Princeton, New Jersey 08540, USA
    Phys Rev Lett 93:228103. 2004
    ..We provide a model for the polar localization of MinD based only on diffusion, a delay for nucleotide exchange, and different rates of attachment to the bare membrane ..
  75. ncbi A conserved polar region in the cell division site determinant MinD is required for responding to MinE-induced oscillation but not for localization within coiled arrays
    Jason Szeto
    Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, K1H 8M5, Canada
    Res Microbiol 156:17-29. 2005
    A region in the cell division site determinant MinD required for stimulation by MinE and which determines MinD topological specificity along coil-like structures has been identified...
  76. pmc Noise-induced Min phenotypes in E. coli
    David Fange
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden
    PLoS Comput Biol 2:e80. 2006
    The spatiotemporal oscillations of the Escherichia coli proteins MinD and MinE direct cell division to the region between the chromosomes...
  77. pmc A multistranded polymer model explains MinDE dynamics in E. coli cell division
    Eric N Cytrynbaum
    Department of Mathematics, University of British Columbia, Vancouver, British Columbia, Canada
    Biophys J 93:1134-50. 2007
    ..These proteins undergo a periodic pole-to-pole oscillation that involves polymerization and ATPase activity of MinD under the controlling influence of MinE...
  78. doi Mesoscale modeling technique for studying the dynamics oscillation of Min protein: pattern formation analysis with lattice Boltzmann method
    Somchai Sriyab
    Department of Mathematics, Faculty of Science, Mahidol University, Bangkok, Thailand
    Comput Biol Med 39:412-24. 2009
    ..The oscillations involve MinC, MinD and MinE proteins, which are required for proper placement of the division septum in the middle of a bacterial cell...
  79. pmc Differences in MinC/MinD sensitivity between polar and internal Z rings in Escherichia coli
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 193:367-76. 2011
    ..In the current model for the Min system, the MinC/MinD division inhibitory complex is evenly distributed on the membrane and can disrupt Z rings anywhere in the cell; ..
  80. ncbi The Escherichia coli minB mutation resembles gyrB in defective nucleoid segregation and decreased negative supercoiling of plasmids
    E Mulder
    Department of Molecular Cell Biology, University of Amsterdam, The Netherlands
    Mol Gen Genet 221:87-93. 1990
    Nucleoid segregation in the Escherichia coli minB mutant and in cells that over-produce minB gene products appeared defective as measured from fluorescence micrographs...
  81. pmc Interaction between the min locus and ftsZ
    E Bi
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 172:5610-6. 1990
    In Escherichia coli, distinct but similar minicell phenotypes resulting from mutation at the minB locus and increased expression of ftsZ suggested a possible interaction between these genes...
  82. pmc Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene product
    C Labie
    Centre de Recherches de Biochimie et de Génétique cellulaires du Centre National de la Recherche Scientifique, Toulouse, France
    J Bacteriol 172:5852-5. 1990
    ..All the mutations were found to lie in minC or minD, confirming the requirement of both gene products in the process of inhibition of polar sites...
  83. ncbi Isolation of a Francisella tularensis mutant that is sensitive to serum and oxidative killing and is avirulent in mice: correlation with the loss of MinD homologue expression
    L S Anthony
    Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
    FEMS Microbiol Lett 124:157-65. 1994
    ..DNA sequence analysis at the KmR insertion site suggested that the F. tularensis homologue of minD had been interrupted. Western immunoblot analysis confirmed the presence of a MinD homologue in F...
  84. pmc MinCD proteins control the septation process during sporulation of Bacillus subtilis
    I Barak
    Institute of Molecular Biology, Slovak Academy of Sciences, 841 51 Bratislava, Slovak Republic
    J Bacteriol 180:5327-33. 1998
    ..The divIVB locus contains five open reading frames (ORFs). The last two ORFs (minCD) are homologous to minC and minD of Escherichia coli but a minE homolog is lacking in B. subtilis...
  85. ncbi [Progressing molecular cell biology in bacteria: chromosome partitioning and cell division]
    H Niki
    Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan
    Tanpakushitsu Kakusan Koso 45:153-63. 2000
  86. pmc Analysis of MinC reveals two independent domains involved in interaction with MinD and FtsZ
    Z Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    J Bacteriol 182:3965-71. 2000
    ..The C-terminal domain interacts with MinD, and expression in wild-type cells as a MalE fusion disrupts min function, resulting in a minicell phenotype...
  87. ncbi FtsZ ring formation without subsequent cell division after replication runout in Escherichia coli
    B Gullbrand
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Sweden
    Mol Microbiol 36:1349-59. 2000
    ..This finding correlates well with the previously observed oscillatory movement of MinC and MinD in short and long cells.
  88. ncbi How does a bacterium find its middle?
    D RayChaudhuri
    Nat Struct Biol 7:997-9. 2000
  89. pmc Cytokinesis in bacteria
    Jeffery Errington
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Microbiol Mol Biol Rev 67:52-65, table of contents. 2003
    ..The functions of most of these proteins are unclear with the exception of at least one penicillin-binding protein, which catalyzes a key step in cell wall synthesis in the division septum...
  90. ncbi MinD and role of the deviant Walker A motif, dimerization and membrane binding in oscillation
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 48:295-303. 2003
    The ATPase activity of MinD is required for it to oscillate between the ends of the cell and spatially regulate cell division in Escherichia coli...
  91. ncbi A hypothesis to explain division site selection in Escherichia coli by combining nucleoid occlusion and Min
    Vic Norris
    Assemblages Moléculaires Modélisation et Imagerie SIMS, FRE CNRS 2829, Faculte des Sciences and Techniques, Universite de Rouen, 76821 Mont Saint Aignan, France
    FEBS Lett 561:3-10. 2004
    ..of proteins into membrane (transertion), can structure the cytoplasmic membrane into phospholipid domains, (2) the MinD protein can convert vesicles into tubes and (3) a variety of membranous structures can be observed at mid-cell...
  92. pmc ZipA is required for targeting of DMinC/DicB, but not DMinC/MinD, complexes to septal ring assemblies in Escherichia coli
    Jay E Johnson
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4960, USA
    J Bacteriol 186:2418-29. 2004
    ..FtsZ assembly, while the C-terminal domain ((D)MinC) mediates both dimerization and complex formation with either MinD or DicB...
  93. pmc Conserved glycines in the C terminus of MinC proteins are implicated in their functionality as cell division inhibitors
    S Ramirez-Arcos
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    J Bacteriol 186:2841-55. 2004
    ..Each mutant was evaluated for its ability to arrest cell division and to interact with itself and MinD. In contrast to overexpression of wild-type MinC, overexpression of mutant proteins in E...
  94. ncbi Microbiology. Dynamic instability of a bacterial engine
    Jakob Møller-Jensen
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    Science 306:987-9. 2004
  95. ncbi Spatial control of bacterial division-site placement
    Lawrence Rothfield
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Rev Microbiol 3:959-68. 2005
    ..Here, we review the latest findings on these two systems...
  96. ncbi Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy
    G Meacci
    Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
    Phys Biol 3:255-63. 2006
    In the bacterium Escherichia coli, selection of the division site involves pole-to-pole oscillations of the proteins MinD and MinE...
  97. pmc Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding
    Shirley Mazor
    Department of Life Sciences, Ben Gurion University of the Negev, P O B 653, Beer Sheva 84105, Israel
    Biochim Biophys Acta 1778:2505-11. 2008
    b>MinD, a well-conserved bacterial amphitropic protein involved in spatial regulation of cell division, has a typical feature of reversible binding to the membrane...
  98. doi Modelling of processes governing subcellular localisation of MinD in Escherichia coli
    X Xu
    Virginia Polytechnic and State University, Department of Physics, Blacksburg, VA 24061, USA
    IET Syst Biol 2:285-92. 2008
    ..these models use varying assumptions to do so leading to different mechanisms for precise polar localisation of MinD zones...
  99. pmc The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 72:410-24. 2009
    ..MinC is a cell division inhibitor whose activity is spatially regulated by MinD and MinE...
  100. pmc Intra- and intercellular fluctuations in Min-protein dynamics decrease with cell length
    Elisabeth Fischer-Friedrich
    Max Planck Institute for the Physics of Complex Systems, Nothnitzer Strasse 38, 01187 Dresden, Germany
    Proc Natl Acad Sci U S A 107:6134-9. 2010
    ..As the pattern becomes more regular, cell-to-cell variability also lessens, indicating cell length-dependent regulation of Min-protein activity...
  101. ncbi The Bacillus subtilis DivIVA protein targets to the division septum and controls the site specificity of cell division
    D H Edwards
    Sir William Dunn School of Pathology, University of Oxford, UK
    Mol Microbiol 24:905-15. 1997
    ..A DivIVA-GFP fusion targets to new and used sites of cell division, consistent with it having a direct role in topological specification...

Research Grants3

  1. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
    ..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..
  2. BACTERIAL CELL DIVISION
    Lawrence Rothfield; Fiscal Year: 2002
    ..4. To determine whether the residual division sites that are present at the cell poles play a role in the generation of future division sites within the cell. ..
  3. E COLI MIN PROTEINS
    Lawrence Rothfield; Fiscal Year: 2003
    ..Since the products of the three min genes, minC, minD and minE, are responsible for this site-selection process, we will attempt to define the mechanism of action of ..