mscL

Summary

Gene Symbol: mscL
Description: mechanosensitive channel protein, high conductance
Alias: ECK3277, JW3252, stkA, yhdC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
  2. ncbi Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli
    C C Häse
    Department of Pharmacology, University of Western Australia, Nedlands
    J Biol Chem 270:18329-34. 1995
  3. pmc Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Proc Natl Acad Sci U S A 93:11652-7. 1996
  4. pmc Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706, USA
    EMBO J 15:4798-805. 1996
  5. ncbi Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria
    P Blount
    Laboratory of Molecular Biology, Bock Labs, University of Wisconsin Madison 53706, USA
    Biol Cell 87:1-8. 1996
  6. ncbi Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Annu Rev Physiol 59:633-57. 1997
  7. pmc Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli
    C C Cruickshank
    Department of Pharmacology, University of Western Australia, Nedlands
    Biophys J 73:1925-31. 1997
  8. ncbi Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:32150-7. 1997
  9. ncbi Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
    P C Moe
    Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin Madison, 53706, USA
    Mol Microbiol 28:583-92. 1998
  10. ncbi Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel
    G Chang
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Science 282:2220-6. 1998

Research Grants

Scientific Experts

Detail Information

Publications115 found, 100 shown here

  1. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
    ..experiments with Escherichia coli envelope have revealed a mechanosensitive channel with very large conductance (MscL) and one with a smaller conductance (MscS) which may be important in osmoregulation...
  2. ncbi Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli
    C C Häse
    Department of Pharmacology, University of Western Australia, Nedlands
    J Biol Chem 270:18329-34. 1995
    The large mechanosensitive ion channel (MscL) of Escherichia coli was expressed on a plasmid encoding MscL as a fusion protein with glutathione S-transferase in an Escherichia coli strain containing a disruption in the chromosomal mscL ..
  3. pmc Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Proc Natl Acad Sci U S A 93:11652-7. 1996
    b>MscL is a channel that opens a large pore in the Escherichia coli cytoplasmic membrane in response to mechanical stress...
  4. pmc Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706, USA
    EMBO J 15:4798-805. 1996
    We have studied the membrane topology and multimeric structure of a mechanosensitive channel, MscL, which we previously isolated and cloned from Escherichia coli...
  5. ncbi Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria
    P Blount
    Laboratory of Molecular Biology, Bock Labs, University of Wisconsin Madison 53706, USA
    Biol Cell 87:1-8. 1996
    ..A gene called mscL, which was isolated from Escherichia coli, was the first gene shown to encode a mechanosensitive channel activity...
  6. ncbi Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Annu Rev Physiol 59:633-57. 1997
    ..responses are ubiquitous and diverse, the molecular bases of mechanosensation in most cases remain mysterious MscL, a mechanosensitive channel of large conductance of Escherichia coli and its bacterial homologues are the first and ..
  7. pmc Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli
    C C Cruickshank
    Department of Pharmacology, University of Western Australia, Nedlands
    Biophys J 73:1925-31. 1997
    The open channel diameter of Escherichia coli recombinant large-conductance mechanosensitive ion channels (MscL) was estimated using the model of Hille (Hille, B. 1968. Pharmacological modifications of the sodium channels of frog nerve. J...
  8. ncbi Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:32150-7. 1997
    b>MscL is a channel found in bacterial plasma membranes that opens a large pore in response to mechanical stress...
  9. ncbi Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
    P C Moe
    Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin Madison, 53706, USA
    Mol Microbiol 28:583-92. 1998
    b>mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge...
  10. ncbi Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel
    G Chang
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Science 282:2220-6. 1998
    ..The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate ..
  11. pmc Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL
    S I Sukharev
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    J Gen Physiol 113:525-40. 1999
    b>MscL is multimeric protein that forms a large conductance mechanosensitive channel in the inner membrane of Escherichia coli...
  12. pmc Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity
    N Levina
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK
    EMBO J 18:1730-7. 1999
    ..Electrophysiological measurements have identified multiple channels in Escherichia coli cells. One gene, mscL, encoding a large conductance channel has previously been described, but null mutants were without well-defined ..
  13. ncbi Channel gate! Tension, leak and disclosure
    A F Batiza
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Structure 7:R99-103. 1999
    The crystal structure of a bacterial MscL shows how this homopentameric channel protein is held tightly shut to prevent leakage whilst at rest...
  14. ncbi 'Feeling the pressure': structural insights into a gated mechanosensitive channel
    R H Spencer
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Opin Struct Biol 9:448-54. 1999
    The structure determination of the large-conductance mechanosensitive channel (MscL) from Mycobacterium tuberculosis has revealed the architecture of the first full-length, gated pentameric ion channel...
  15. ncbi Comparing and contrasting Escherichia coli and Mycobacterium tuberculosis mechanosensitive channels (MscL). New gain of function mutations in the loop region
    J A Maurer
    Divisions of Chemistry and Chemical Engineering and Biology, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 275:22238-44. 2000
    Sequence analysis of 35 putative MscL homologues was used to develop an optimal alignment for Escherichia coli and Mycobacterium tuberculosis MscL and to place these homologues into sequence subfamilies...
  16. ncbi The gating mechanism of the large mechanosensitive channel MscL
    S Sukharev
    Department of Biology, University of Maryland, College Park 20742, USA
    Nature 409:720-4. 2001
    The mechanosensitive channel of large conductance, MscL, is a ubiquitous membrane-embedded valve involved in turgor regulation in bacteria...
  17. pmc Structural determinants of MscL gating studied by molecular dynamics simulations
    J Gullingsrud
    Beckman Institute, Department of Physics, University of Illinois, 405 N Mathews Avenue, Urbana, Illinois 61801, USA
    Biophys J 80:2074-81. 2001
    The mechanosensitive channel of large conductance (MscL) in prokaryotes plays a crucial role in exocytosis as well as in the response to osmotic downshock. The channel can be gated by tension in the membrane bilayer...
  18. pmc Chemically charging the pore constriction opens the mechanosensitive channel MscL
    K Yoshimura
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 0033, Japan
    Biophys J 80:2198-206. 2001
    b>MscL is a bacterial mechanosensitive channel that protects the cell from osmotic downshock...
  19. pmc Structural models of the MscL gating mechanism
    S Sukharev
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    Biophys J 81:917-36. 2001
    Three-dimensional structural models of the mechanosensitive channel of large conductance, MscL, from the bacteria Mycobacterium tuberculosis and Escherichia coli were developed for closed, intermediate, and open conformations...
  20. ncbi A large iris-like expansion of a mechanosensitive channel protein induced by membrane tension
    Monica Betanzos
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    Nat Struct Biol 9:704-10. 2002
    b>MscL, a bacterial mechanosensitive channel of large conductance, is the first structurally characterized mechanosensor protein. Molecular models of its gating mechanisms are tested here...
  21. ncbi Open channel structure of MscL and the gating mechanism of mechanosensitive channels
    Eduardo Perozo
    Department of Molecular Physiology and Biological Physics, and Center for Structural Biology, University of Virginia, Charlottesville, Virginia 22906, USA
    Nature 418:942-8. 2002
    ..the structural rearrangements that underlie these events in the large prokaryotic mechanosensitive channel (MscL) using electron paramagnetic resonance spectroscopy and site-directed spin labelling...
  22. pmc Two families of mechanosensitive channel proteins
    Christopher D Pivetti
    Division of Biology, University of California San Diego, La Jolla, California 92093 0116, USA
    Microbiol Mol Biol Rev 67:66-85, table of contents. 2003
    ..Two families of ubiquitous MS channels are recognized, and these have been designated the MscL and MscS families...
  23. ncbi Prokaryotic mechanosensitive channels
    Pavel Strop
    California Institute of Technology, Pasadena, California 91125, USA
    Adv Protein Chem 63:177-209. 2003
  24. pmc A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS
    Neil R Stokes
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 100:15959-64. 2003
    The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase...
  25. pmc Purification and functional reconstitution of N- and C-halves of the MscL channel
    Kyu Ho Park
    Unité Mixte de Recherche Centre National de la Recherche Scientifique 8619, Universite Paris Sud, 91405, Orsay Cedex, France
    Biophys J 86:2129-36. 2004
    b>MscL is a mechanosensitive channel gated by membrane tension in the lipid bilayer alone. Its structure, known from x-ray crystallography, indicates that it is a homopentamer...
  26. pmc Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses
    Chien Sung Chiang
    Department of Biology, University of Maryland College Park, Maryland 20742, USA
    Biophys J 86:2846-61. 2004
    ..Despite increasing evidence for the open-state conformation of MscL, the energetic description of its complex gating remains incomplete...
  27. ncbi Mechanosensitive channels: what can we learn from 'simple' model systems?
    Sergei Sukharev
    Biology Department, University of Maryland, College Park, MD 20742, USA
    Trends Neurosci 27:345-51. 2004
    ..Here, the conformational transition driven by membrane tension in the bacterial channel MscL is discussed...
  28. pmc Gating transitions in bacterial ion channels measured at 3 microns resolution
    George Shapovalov
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Gen Physiol 124:151-61. 2004
    ..Two examples, the Escherichia coli mechanosensitive ion channels Ec-MscS and Ec-MscL, pass currents of 125-300 pA...
  29. pmc Membrane-protein interactions in mechanosensitive channels
    Paul Wiggins
    Department of Physics, California Institute of Technology, Pasadena, California 91106, USA
    Biophys J 88:880-902. 2005
    ..conformation and function with specific reference to the case of the mechanosensitive channel of large conductance (MscL)...
  30. pmc Conformational changes involved in MscL channel gating measured using FRET spectroscopy
    Ben Corry
    School of Biomedical, Biomolecular and Chemical Science, The University of Western Australia, Crawley, Australia
    Biophys J 89:L49-51. 2005
    ..The diameter of the protein is found to increase by 16 A upon channel activation...
  31. pmc Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL
    Geert van den Bogaart
    Biochemistry Department, Groningen Biomolecular Science and Biotechnology Institute and Materials Science Centre, University of Groningen, Groningen, The Netherlands
    Biophys J 92:1233-40. 2007
    The mechanosensitive channel protein of large conductance, MscL, from Escherichia coli has been implicated in protein efflux, but the passage of proteins through the channel has never been demonstrated...
  32. pmc Structure of a tetrameric MscL in an expanded intermediate state
    Zhenfeng Liu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nature 461:120-4. 2009
    ..In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances...
  33. pmc OCAM: a new tool for studying the oligomeric diversity of MscL channels
    Chris S Gandhi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Protein Sci 20:313-26. 2011
    ..We have applied OCAM to study the mechanosensitive channel of large conductance (MscL) and find that these proteins can exist in multiple oligomeric states ranging from tetramers up to possible ..
  34. pmc Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS
    Takeshi Nomura
    Molecular Cardiology and Biophysics Divison Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales 2010, Australia
    Proc Natl Acad Sci U S A 109:8770-5. 2012
    Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock...
  35. pmc Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
    Biophys J 65:177-83. 1993
    ....
  36. ncbi Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity
    C C Häse
    Department of Pharmacology, University of Western Australia, Nedlands WA 6907, Australia
    J Membr Biol 157:17-25. 1997
    In the search for the essential functional domains of the large mechanosensitive ion channel (MscL) of E. coli, we have cloned several mutants of the mscL gene into a glutathione S-transferase fusion protein expression system...
  37. pmc One face of a transmembrane helix is crucial in mechanosensitive channel gating
    X Ou
    Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:11471-5. 1998
    b>MscL is a mechanosensitive channel in bacteria that responds directly to membrane tension by opening a large conductance pore...
  38. ncbi Release of thioredoxin via the mechanosensitive channel MscL during osmotic downshock of Escherichia coli cells
    B Ajouz
    Laboratoire des Biomembranes, ERS CNRS 571, Bat 430, Université Paris Sud 91405 Orsay Cedex, France
    J Biol Chem 273:26670-4. 1998
    Escherichia coli cells possess several mechanosensitive ion channels but only MscL, the channel with the highest conductance, which is activated at the highest membrane tension, has been cloned...
  39. ncbi Stoichiometry of the large conductance bacterial mechanosensitive channel of E. coli. A biochemical study
    S I Sukharev
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Membr Biol 171:183-93. 1999
    b>MscL, a 15 kDa transmembrane protein, is the only component involved in the formation of a 3 nS channel in the inner membrane of Escherichia coli that opens in response to mechanical or osmotic stress...
  40. pmc Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity
    K Yoshimura
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 77:1960-72. 1999
    Mechanosensitive channel large (MscL) encodes the large conductance mechanosensitive channel of the Escherichia coli inner membrane that protects bacteria from lysis upon osmotic shock...
  41. ncbi Contributions of the different extramembranous domains of the mechanosensitive ion channel MscL to its response to membrane tension
    B Ajouz
    Laboratoire des Biomembranes, Unite Mixte de Recherche CNRS 8619, Batiment 430, Université Paris Sud 91405 Orsay Cedex France
    J Biol Chem 275:1015-22. 2000
    b>MscL is a mechanosensitive channel that is gated by tension in the membrane bilayer alone...
  42. ncbi Mechanosensitive channels in prokaryotes
    B Martinac
    Department of Pharmacology, University of Western Australia, Nedlands, Australia
    Cell Physiol Biochem 11:61-76. 2001
    ..Cloning of MscL and MscS, the MS channels of Large and Small conductance found in Bacteria and elucidation of the 3D crystal ..
  43. pmc Site-directed spin-labeling analysis of reconstituted Mscl in the closed state
    E Perozo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22906, USA
    J Gen Physiol 118:193-206. 2001
    The mechanosensitive channel from Escherichia coli (Eco-MscL) responds to membrane lateral tension by opening a large, water-filled pore that serves as an osmotic safety valve...
  44. ncbi The alpha-helix and the organization and gating of channels
    Robert H Spencer
    Department of Pharmacology, Merck Research Laboratories, West Point, PA 19486
    Annu Rev Biophys Biomol Struct 31:207-33. 2002
    ..and other alpha-helical membrane proteins have been determined recently, including the KcsA potassium channel, the MscL mechanosensitive channel, and the AQP1 and GlpF members of the aquaporin family...
  45. ncbi Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis
    Kuniyuki Okada
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9040, USA
    J Biol Chem 277:27682-8. 2002
    MscS and MscL are mechanosensitive channels found in bacterial plasma membranes that open large pores in response to membrane tension. These channels function to alleviate excess cell turgor invoked by rapid osmotic downshock...
  46. ncbi Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating
    Eduardo Perozo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22906, USA
    Nat Struct Biol 9:696-703. 2002
    ..Structural changes in the Escherichia coli large MS channel (MscL) were studied under nominally zero transbilayer pressures using both patch clamp and EPR spectroscopic approaches...
  47. pmc On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL
    Andriy Anishkin
    Department of Biology, University of Maryland College Park, 20742, USA
    J Gen Physiol 121:227-44. 2003
    COOH-terminal (S3) domains are conserved within the MscL family of bacterial mechanosensitive channels, but their function remains unclear...
  48. pmc Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis
    Daniel Clayton
    Gryphon Therapeutics, 600 Gateway Boulevard, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 101:4764-9. 2004
    ..generate multimilligram quantities of the mechanosensitive channel of large conductance from Escherichia coli (Ec-MscL) and Mycobacterium tuberculosis (Tb-MscL)...
  49. ncbi The role of the periplasmic loop residue glutamine 65 for MscL mechanosensitivity
    I Jung Tsai
    School of Medicine and Pharmacology, University of Western Australia, QE II Medical Center, Nedlands, WA 6009, Australia
    Eur Biophys J 34:403-12. 2005
    The periplasmic loop of MscL, the mechanosensitive channel of large conductance, acts as a spring resisting the opening of the channel...
  50. ncbi Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli
    I T Arkin
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Biochim Biophys Acta 1369:131-40. 1998
    In this report, we present structural studies on the large conductance mechanosensitive ion channel (MscL) from E. coli in detergent micelles and lipid vesicles...
  51. pmc Electromechanical coupling model of gating the large mechanosensitive ion channel (MscL) of Escherichia coli by mechanical force
    L Gu
    Department of Pharmacology, University of Western Australia, Nedlands, Australia
    Biophys J 74:2889-902. 1998
    ..theoretical electromechanical coupling (EMC) model of gating of the large-conductance mechanosensitive ion channel (MscL)...
  52. ncbi Generation and evaluation of a large mutational library from the Escherichia coli mechanosensitive channel of large conductance, MscL: implications for channel gating and evolutionary design
    Joshua A Maurer
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 278:21076-82. 2003
    Random mutagenesis of the mechanosensitive channel of large conductance (MscL) from Escherichia coli coupled with a high-throughput functional screen has provided new insights into channel structure and function...
  53. ncbi Structure and mechanism in prokaryotic mechanosensitive channels
    Eduardo Perozo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Curr Opin Struct Biol 13:432-42. 2003
    ..The X-ray crystal structures of MscL and MscS offer a unique opportunity to identify the types of protein motions associated with the opening and ..
  54. pmc Gating of MscL studied by steered molecular dynamics
    Justin Gullingsrud
    Department of Physics and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 85:2087-99. 2003
    Steered molecular dynamics simulations of the mechanosensitive channel of large conductance, MscL, were used to investigate how forces arising from membrane tension induce gating of the channel...
  55. ncbi Mechanosensitive channels of bacteria and archaea share a common ancestral origin
    Anna Kloda
    Department of Pharmacology, QEII Medical Center, The University of Western Australia, Crawley
    Eur Biophys J 31:14-25. 2002
    ..Methanococcus jannaschii by using the TM1 transmembrane domain of the bacterial MS channel of large conductance, MscL, as a genetic probe to search the archaeal genomic database for MS channel homologues...
  56. pmc Molecular dynamics study of MscL interactions with a curved lipid bilayer
    Grischa R Meyer
    School of Medicine and Pharmacology, QEII Medical Centre, University of Western Australia, Crawley WA 6009, Australia
    Biophys J 91:1630-7. 2006
    ..mechanism is found in bacteria in the form of the mechanosensitive membrane channel of large conductance, MscL. This channel has been studied extensively using a variety of methods at a functional and structural level...
  57. ncbi On-resin assembly of a linkerless lanthanide(III)-based luminescence label and its application to the total synthesis of site-specifically labeled mechanosensitive channels
    Christian F W Becker
    Gryphon Therapeutics, 600 Gateway Boulevard, South San Francisco, California 94080, USA
    Bioconjug Chem 15:1118-24. 2004
    ..of this label into a chemically synthesized, full-length mechanosensitive channel of large conductance (MscL) of E...
  58. pmc Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL
    C Berrier
    Laboratoire des Biomembranes, UMR CNRS 8619, Universite Paris Sud, 91405 Orsay Cedex, France
    J Bacteriol 182:248-51. 2000
    ..We have shown previously that deletion of mscL, the gene coding for the mechanosensitive channel of the plasma membrane with the highest conductance, prevents the ..
  59. pmc Gating the bacterial mechanosensitive channel MscL invivo
    Ann Finney Batiza
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 99:5643-8. 2002
    YggB and MscL are the major mechanosensitive channels in Escherichia coli, and each can rescue the double knockout mutant from osmotic downshock...
  60. pmc Analyses of circular dichroism spectra of membrane proteins
    B A Wallace
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Protein Sci 12:875-84. 2003
    ..These results have important consequences for studies that seek to use the existing soluble protein reference databases for the analyses of membrane proteins...
  61. ncbi Channels in microbes: so many holes to fill
    Ching Kung
    Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, WI, USA
    Mol Microbiol 53:373-80. 2004
    ..The intellectual and technical schisms between 'neuro' and 'micro' biology must be bridged before we know how we became so smart, and whether microbes are just as smart...
  62. ncbi Lipid-mediated light activation of a mechanosensitive channel of large conductance
    Joost H A Folgering
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Langmuir 20:6985-7. 2004
    ..For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-..
  63. pmc Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:1040-50. 2005
    ..In addition, we identified a few small RNAs that are expressed opposite protein-coding genes and could base pair with 5' or 3' ends of the mRNAs with perfect complementarity...
  64. ncbi Visualisation of the mechanosensitive channel of large conductance in bacteria using confocal microscopy
    Christel Norman
    School of Medicine and Pharmacology, QEII Medical Centre, The University of Western Australia, Crawley, WA 6009, Australia
    Eur Biophys J 34:396-402. 2005
    The mechanosensitive channel of large conductance (MscL) plays an important role in the survival of bacterial cells to hypo-osmotic shock...
  65. ncbi A light-actuated nanovalve derived from a channel protein
    Armagan Kocer
    BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, Netherlands
    Science 309:755-8. 2005
    ..The valve consists of a channel protein, the mechanosensitive channel of large conductance (MscL) from Escherichia coli, modified by attachment of synthetic compounds that undergo light-induced charge separation ..
  66. pmc Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristics
    Joost H A Folgering
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Protein Sci 14:2947-54. 2005
    ..making single substitutions per channel and to determine the quaternary structure of the mechanosensitive channel MscL from Escherichia coli, covalent oligomers (monomer to hexamer) were engineered by gene fusion; up to six copies of ..
  67. pmc C-terminal charged cluster of MscL, RKKEE, functions as a pH sensor
    Anna Kloda
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Biophys J 90:1992-8. 2006
    ..The RKKEE cluster of charged residues located within the cytoplasmic helix of the bacterial mechanosensitive channel, MscL, is essential for the channel function...
  68. pmc Disulfide trapping the mechanosensitive channel MscL into a gating-transition state
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 92:1224-32. 2007
    The mechanosensitive channel of large conductance, MscL, serves as a biological emergency release valve protecting bacteria from acute osmotic downshock, and is to date the best characterized mechanosensitive channel...
  69. pmc Anionic phospholipids affect the rate and extent of flux through the mechanosensitive channel of large conductance MscL
    Andrew M Powl
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, UK
    Biochemistry 47:4317-28. 2008
    The mechanosensitive channel of large conductance MscL from Escherichia coli has been reconstituted into sealed vesicles, and the effects of lipid structure on the flux of the fluorescent molecule calcein through the open channel have ..
  70. pmc Gating mechanisms of mechanosensitive channels of large conductance, II: systematic study of conformational transitions
    Yuye Tang
    Nanomechanics Research Center, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, New York 10027, USA
    Biophys J 95:581-96. 2008
    ..In Part II, the gating pathways of Escherichia coli-MscL channels under various basic deformation modes are simulated...
  71. pmc An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating
    Yuezhou Li
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    FASEB J 23:2197-204. 2009
    ..tractable nature, a bacterial mechanosensitive channel that serves as an in vivo osmotic "emergency release valve," MscL, has become a paradigm of how a mechanosensitive channel can sense and respond to membrane tension...
  72. pmc Manipulating the permeation of charged compounds through the MscL nanovalve
    Li Min Yang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    FASEB J 25:428-34. 2011
    b>MscL is a bacterial mechanosensor that serves as a biological emergency release valve, releasing cytoplasmic solutes to the environment on osmotic downshock...
  73. ncbi MscL: a mechanosensitive channel in Escherichia coli
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Soc Gen Physiol Ser 51:133-41. 1996
  74. ncbi Cross-linking studies and membrane localization and assembly of radiolabelled large mechanosensitive ion channel (MscL) of Escherichia coli
    C C Häse
    Department of Pharmacology, University of Western Australia, Nedlands, Australia
    Biochem Biophys Res Commun 232:777-82. 1997
    The gene encoding the large conductance mechanosensitive ion channel (MscL) of Escherichia coli and several deletion mutants of mscL were cloned under the control of the T7 RNA polymerase promoter...
  75. ncbi Mechanosensitive channels of bacteria
    P Blount
    Department of Physiology, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Methods Enzymol 294:458-82. 1999
  76. ncbi Managing hypoosmotic stress: aquaporins and mechanosensitive channels in Escherichia coli
    I R Booth
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, UK
    Curr Opin Microbiol 2:166-9. 1999
    ..coli cell membrane is well documented. The recent identification of genes that inactivate the MscL and MscS channels has established their role in releasing the pressure built up by hypoosmotic shock...
  77. ncbi Mechanosensitive channels in bacteria as membrane tension reporters
    S Sukharev
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    FASEB J 13:S55-61. 1999
    The purpose of this short review is to discuss recent data on the molecular structure and mechanism of gating of MscL, a mechanosensitive channel of large conductance from Escherichia coli...
  78. pmc Structure and function of the bacterial mechanosensitive channel of large conductance
    A J Oakley
    Department of Pharmacology, Crystallography Center, University of Western Australia, Nedlands, Australia
    Protein Sci 8:1915-21. 1999
    ..In Escherichia coli and other bacteria, this function is carried out by a number of proteins including MscL, the mechanosensitive channel of large conductance. MscL is the best characterized of all mechanosensitive channels...
  79. ncbi A mechanosensitive channel protein and its gene in E. coli
    P Blount
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Gravit Space Biol Bull 10:43-7. 1997
    ..We have discovered in E. coli the activity of a mechanosensitive ion channel of large conductance (MscL), and have successfully isolated the corresponding protein and gene (Sukharev et al. 1994a)...
  80. pmc Conformational pathways in the gating of Escherichia coli mechanosensitive channel
    Yifei Kong
    Graduate Program of Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, BCM 125, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:5999-6004. 2002
    ..Moreover, the intermediate open states of mechanosensitive channels are not symmetric, i.e., the opening does not follow iris-like motion, which sharply contrasts to the potassium channel KcsA...
  81. ncbi Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli
    Debra McLaggan
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, UK
    Mol Microbiol 43:521-36. 2002
    ..In Escherichia coli, there are multiple mechanosensitive channels that have been characterized genetically: MscL, YggB and KefA...
  82. ncbi Retaining your identity under stress
    Frederick Sachs
    Nat Struct Biol 9:636-7. 2002
  83. pmc Investigating lipid composition effects on the mechanosensitive channel of large conductance (MscL) using molecular dynamics simulations
    Donald E Elmore
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biophys J 85:1512-24. 2003
    ..experimental work has shown that the functional properties of the mechanosensitive channel of large conductance (MscL) are affected by variations in lipid composition...
  84. ncbi Dynamical properties of the MscL of Escherichia coli: a normal mode analysis
    H Valadié
    Equipe de Bioinformatique Genomique et Moleculaire, EMI03 46, Universite Paris 7, 2, place Jussieu, case 7113, 75251 Cedex 05, Paris, France
    J Mol Biol 332:657-74. 2003
    The mechanosensitive channel (MscL) is an integral membrane protein which gates in response to membrane tension...
  85. pmc Analytic models for mechanotransduction: gating a mechanosensitive channel
    Paul Wiggins
    Division of Physics, Mathematics, and Astronomy, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125 9500, USA
    Proc Natl Acad Sci U S A 101:4071-6. 2004
    ..for the forces and free energy generated by bilayer deformation reveal a compelling and intuitive model for MscL channel gating analogous to the nucleation of a second phase...
  86. pmc Cysteine scanning of MscL transmembrane domains reveals residues critical for mechanosensitive channel gating
    Gal Levin
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 86:2862-70. 2004
    The mechanosensitive channel of large conductance (MscL), a bacterial channel, is perhaps the best characterized mechanosensitive protein...
  87. ncbi Assessment of potential stimuli for mechano-dependent gating of MscL: effects of pressure, tension, and lipid headgroups
    Paul Moe
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9040, USA
    Biochemistry 44:12239-44. 2005
    b>MscL is a mechanosensitive channel of large conductance that serves as an "emergency relief valve", protecting bacteria from acute hypoosmotic stress...
  88. pmc A finite element framework for studying the mechanical response of macromolecules: application to the gating of the mechanosensitive channel MscL
    Yuye Tang
    Department of Civil Engineering and Engineering Mechanics, Nanomechanics Research Center, Columbia University, New York, New York, USA
    Biophys J 91:1248-63. 2006
    The gating pathways of mechanosensitive channels of large conductance (MscL) in two bacteria (Mycobacterium tuberculosis and Escherichia coli) are studied using the finite element method...
  89. ncbi The mechanosensitive channel protein MscL is targeted by the SRP to the novel YidC membrane insertion pathway of Escherichia coli
    Sandra J Facey
    Institute of Microbiology, University of Hohenheim, 70599 Stuttgart, Germany
    J Mol Biol 365:995-1004. 2007
    The mechanosensitive channel MscL in the inner membrane of Escherichia coli is a homopentameric complex involved in homeostasis when cells are exposed to hypo-osmotic conditions. The E...
  90. pmc Penetration of lipid chains into transmembrane surfaces of membrane proteins: studies with MscL
    Joanne Carney
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, United Kingdom
    Biophys J 92:3556-63. 2007
    ..lipid-facing sites in the first transmembrane alpha-helix (M1) of the mechanosensitive channel of large-conductance MscL; lipid-facing residues at the N-terminal end of M1 are buried below the transmembrane surface of the protein...
  91. ncbi Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane
    Martin Andersson
    Department of Chemistry, University of Florida, Gainesville, FL, USA
    Biosens Bioelectron 23:919-23. 2008
    ..Here, we have for the first time analyzed the large conducting (MscL) channel in a supported membrane using only an external electrical field...
  92. pmc Confirming the revised C-terminal domain of the MscL crystal structure
    Joshua A Maurer
    Department of Chemistry, Washington University, St Louis, Missouri 63130, USA
    Biophys J 94:4662-7. 2008
    The structure of the C-terminal domain of the mechanosensitive channel of large conductance (MscL) has generated significant controversy...
  93. pmc On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface
    Irene Iscla
    Department of Physiology, University of Texas, Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    Biophys J 95:2283-91. 2008
    The mechanosensitive channel of large conductance, MscL, serves as a biological emergency release valve protecting bacteria from acute osmotic downshock and is to date the best characterized mechanosensitive channel...
  94. pmc Mechanosensitive channels: insights from continuum-based simulations
    Yuye Tang
    Department of Civil Engineering and Engineering Mechanics, Nanomechanics Research Center, Columbia University, New York, NY 10027, USA
    Cell Biochem Biophys 52:1-18. 2008
    ..and gating behaviors of a prototypical mechanosensitive channel, the mechanosensitive channel of large conductance (MscL) in bacteria Escherichia coli (E...
  95. doi Importance of direct interactions with lipids for the function of the mechanosensitive channel MscL
    Andrew M Powl
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 47:12175-84. 2008
    We have studied the effects of lipid structure on the function of the mechanosensitive channel of large conductance (MscL) from Escherichia coli to determine whether effects follow from direct interaction between the lipids and protein or ..
  96. ncbi NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport
    A Schlosser
    Abteilung Mikrobiologie, Universitat Osnabruck, Germany
    Mol Microbiol 9:533-43. 1993
    ..Strong u.v. illumination at 252 nm led to cross-linking of NAD+ or NADH, but not of ATP to the isolated TrkA protein...
  97. ncbi Mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyticus, by osmotic downshock
    Y Nakamaru
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 444:170-2. 1999
    ..We introduced an mscL gene into Vibrio alginolyticus, and the mechanosensitive channel with large conductance functions was found to ..
  98. pmc Osmosensing by bacteria: signals and membrane-based sensors
    J M Wood
    Department of Microbiology and Guelph Waterloo Centre for Graduate Work in Chemistry, University of Guelph, Guelph, Ontario, Canada N1G
    Microbiol Mol Biol Rev 63:230-62. 1999
    ..The osmoprotectant transporter ProP and channel MscL act as osmosensors after purification and reconstitution in proteoliposomes...
  99. ncbi Bacterial mechanosensitive channels: integrating physiology, structure and function
    P Blount
    Dept of Physiology, University of Texas Southwestern Medical Centre, Dallas, TX 75235 9040, USA
    Trends Microbiol 7:420-4. 1999
    ..Mutagenesis of one of these channels, combined with structural information derived from X-ray crystallography, has given the first clues of how a mechanosensitive channel senses and responds to membrane tension...

Research Grants2

  1. Functional Cycle of a Mechanosensitive Channel
    Sergei Sukharev; Fiscal Year: 2009
    ..The work, when accomplished, will move us closer toward understanding the mechanisms of the growing families of sensory channels. ..
  2. Roles of Hydration and Lipids in Mechanosensitive Channel Gating
    Sergei Sukharev; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): MscL, a prokaryotic osmolyte release valve is the best understood mechanosensitive channel...