recA

Summary

Gene Symbol: recA
Description: DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Alias: ECK2694, JW2669, lexB, recH, rnmB, srf, tif, umuB, umuR, zab
Species: Escherichia coli str. K-12 substr. MG1655
Products:     recA

Top Publications

  1. Umezu K, Chi N, Kolodner R. Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein. Proc Natl Acad Sci U S A. 1993;90:3875-9 pubmed
    The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates...
  2. Sommer S, Coste G, Bailone A. Specific amino acid changes enhance the anti-recombination activity of the UmuD'C complex. Mol Microbiol. 2000;35:1443-53 pubmed
    ..the UmuD'C complex sensitizes recA+ bacteria to DNA damage, whereas it has no effect on bacteria expressing a RecA [UmuR] protein that overcomes recombination inhibition...
  3. Lusetti S, Voloshin O, Inman R, Camerini Otero R, Cox M. The DinI protein stabilizes RecA protein filaments. J Biol Chem. 2004;279:30037-46 pubmed
    When DinI is present at concentrations that are stoichiometric with those of RecA or somewhat greater, DinI has a substantial stabilizing effect on RecA filaments bound to DNA...
  4. Seigneur M, Ehrlich S, Michel B. RuvABC-dependent double-strand breaks in dnaBts mutants require recA. Mol Microbiol. 2000;38:565-74 pubmed
    ..that, in the rep mutant, RuvABC-mediated breakage occurs in the absence of the homologous recombination protein RecA. In contrast, in dnaBts mutants, most of the RuvABC-mediated breakage depends on the presence of RecA, which ..
  5. Schlacher K, Cox M, Woodgate R, Goodman M. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V. Nature. 2006;442:883-7 pubmed
    The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage...
  6. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, ..
  7. Bianco P, Tracy R, Kowalczykowski S. DNA strand exchange proteins: a biochemical and physical comparison. Front Biosci. 1998;3:D570-603 pubmed
    ..In Escherichia coli, the RecA protein is essential to recombination, and biochemical analysis demonstrates that it is responsible for the crucial ..
  8. Harris D, Pollock S, Wood E, Goiffon R, Klingele A, Cabot E, et al. Directed evolution of ionizing radiation resistance in Escherichia coli. J Bacteriol. 2009;191:5240-52 pubmed publisher
    ..Several mutations in the recA gene and a deletion of the e14 prophage both demonstrably contribute to and partially explain the new phenotype...
  9. Yoshimasu M, Aihara H, Ito Y, Rajesh S, Ishibe S, Mikawa T, et al. An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes. Nucleic Acids Res. 2003;31:1735-43 pubmed
    ..that include DNA damage-induced replication arrest, and is positively regulated by the co- protease activity of RecA. Escherichia coli DinI, a LexA-regulated SOS gene product, shuts off the initiation of the SOS response when ..
  10. Story R, Steitz T. Structure of the recA protein-ADP complex. Nature. 1992;355:374-6 pubmed
    The recA protein catalyses the ATP-driven homologous pairing and strand exchange of DNA molecules...

Detail Information

Publications62

  1. Umezu K, Chi N, Kolodner R. Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein. Proc Natl Acad Sci U S A. 1993;90:3875-9 pubmed
    The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates...
  2. Sommer S, Coste G, Bailone A. Specific amino acid changes enhance the anti-recombination activity of the UmuD'C complex. Mol Microbiol. 2000;35:1443-53 pubmed
    ..the UmuD'C complex sensitizes recA+ bacteria to DNA damage, whereas it has no effect on bacteria expressing a RecA [UmuR] protein that overcomes recombination inhibition...
  3. Lusetti S, Voloshin O, Inman R, Camerini Otero R, Cox M. The DinI protein stabilizes RecA protein filaments. J Biol Chem. 2004;279:30037-46 pubmed
    When DinI is present at concentrations that are stoichiometric with those of RecA or somewhat greater, DinI has a substantial stabilizing effect on RecA filaments bound to DNA...
  4. Seigneur M, Ehrlich S, Michel B. RuvABC-dependent double-strand breaks in dnaBts mutants require recA. Mol Microbiol. 2000;38:565-74 pubmed
    ..that, in the rep mutant, RuvABC-mediated breakage occurs in the absence of the homologous recombination protein RecA. In contrast, in dnaBts mutants, most of the RuvABC-mediated breakage depends on the presence of RecA, which ..
  5. Schlacher K, Cox M, Woodgate R, Goodman M. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V. Nature. 2006;442:883-7 pubmed
    The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage...
  6. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, ..
  7. Bianco P, Tracy R, Kowalczykowski S. DNA strand exchange proteins: a biochemical and physical comparison. Front Biosci. 1998;3:D570-603 pubmed
    ..In Escherichia coli, the RecA protein is essential to recombination, and biochemical analysis demonstrates that it is responsible for the crucial ..
  8. Harris D, Pollock S, Wood E, Goiffon R, Klingele A, Cabot E, et al. Directed evolution of ionizing radiation resistance in Escherichia coli. J Bacteriol. 2009;191:5240-52 pubmed publisher
    ..Several mutations in the recA gene and a deletion of the e14 prophage both demonstrably contribute to and partially explain the new phenotype...
  9. Yoshimasu M, Aihara H, Ito Y, Rajesh S, Ishibe S, Mikawa T, et al. An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes. Nucleic Acids Res. 2003;31:1735-43 pubmed
    ..that include DNA damage-induced replication arrest, and is positively regulated by the co- protease activity of RecA. Escherichia coli DinI, a LexA-regulated SOS gene product, shuts off the initiation of the SOS response when ..
  10. Story R, Steitz T. Structure of the recA protein-ADP complex. Nature. 1992;355:374-6 pubmed
    The recA protein catalyses the ATP-driven homologous pairing and strand exchange of DNA molecules...
  11. McCool J, Long E, Petrosino J, Sandler H, Rosenberg S, Sandler S. Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy. Mol Microbiol. 2004;53:1343-57 pubmed
    ..It is shown that the fluorescence in sulAp-gfp cells is regulated by RecA and LexA. This system was then used to distinguish between the two models for several mutants...
  12. Fujii S, Gasser V, Fuchs R. The biochemical requirements of DNA polymerase V-mediated translesion synthesis revisited. J Mol Biol. 2004;341:405-17 pubmed
    ..In addition, RecA protein and the replication processivity factor, the beta-clamp, were genetically found to be essential co-factors ..
  13. Rangarajan S, Woodgate R, Goodman M. Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins. Mol Microbiol. 2002;43:617-28 pubmed
    ..we have analysed the time dependence of replication restart in strains carrying a combination of mutations in lexA, recA, polB (pol II), umuDC (pol V), priA, dnaC, recF, recO or recR...
  14. Schlacher K, Leslie K, Wyman C, Woodgate R, Cox M, Goodman M. DNA polymerase V and RecA protein, a minimal mutasome. Mol Cell. 2005;17:561-72 pubmed
    ..TLS requires DNA polymerase V (UmuD'2C) and RecA. Here, we show that pol V and RecA interact by two distinct mechanisms...
  15. Ramirez B, Voloshin O, Camerini Otero R, Bax A. Solution structure of DinI provides insight into its mode of RecA inactivation. Protein Sci. 2000;9:2161-9 pubmed publisher
    The Escherichia coli RecA protein triggers both DNA repair and mutagenesis in a process known as the SOS response. The 81-residue E. coli protein DinI inhibits activity of RecA in vivo...
  16. Arnold D, Handa N, Kobayashi I, Kowalczykowski S. A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi. J Mol Biol. 2000;300:469-79 pubmed
    ..This is manifest by both a greater modification of nuclease activity and a higher stimulation of RecA protein-mediated joint molecule formation at chi* than at chi...
  17. Pennington J, Rosenberg S. Spontaneous DNA breakage in single living Escherichia coli cells. Nat Genet. 2007;39:797-802 pubmed
    ..Finally, FACS demonstrated two main cell fates after spontaneous DNA damage: viability with or without resumption of proliferation. ..
  18. Jiang Q, Karata K, Woodgate R, Cox M, Goodman M. The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Nature. 2009;460:359-63 pubmed publisher
    DNA-damage-induced SOS mutations arise when Escherichia coli DNA polymerase (pol) V, activated by a RecA nucleoprotein filament (RecA*), catalyses translesion DNA synthesis...
  19. Courcelle J, Hanawalt P. RecA-dependent recovery of arrested DNA replication forks. Annu Rev Genet. 2003;37:611-46 pubmed
    ..The RecA protein of Escherichia coli is essential for survival in this situation: It maintains the integrity of the arrested ..
  20. Maul R, Sutton M. Roles of the Escherichia coli RecA protein and the global SOS response in effecting DNA polymerase selection in vivo. J Bacteriol. 2005;187:7607-18 pubmed
    ..In contrast, chronically activated RecA protein, expressed from the recA730 allele, is lethal to the dnaN159 strain, and this lethality is suppressed by ..
  21. Godoy V, Jarosz D, Simon S, Abyzov A, Ilyin V, Walker G. UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB. Mol Cell. 2007;28:1058-70 pubmed
    ..We show that the DNA damage-inducible proteins UmuD(2) and RecA act in concert to modulate this mutagenic activity...
  22. Ragone S, Maman J, Furnham N, Pellegrini L. Structural basis for inhibition of homologous recombination by the RecX protein. EMBO J. 2008;27:2259-69 pubmed publisher
    The RecA/RAD51 nucleoprotein filament is central to the reaction of homologous recombination (HR). Filament activity must be tightly regulated in vivo as unrestrained HR can cause genomic instability...
  23. Morimatsu K, Kowalczykowski S. RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair. Mol Cell. 2003;11:1337-47 pubmed
    ..Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby ..
  24. Gill R, Valdes J, Bentley W. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab Eng. 2000;2:178-89 pubmed
    ..increased upregulation of heat shock (ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage life cycle (ftsH, recA) genes...
  25. Story R, Weber I, Steitz T. The structure of the E. coli recA protein monomer and polymer. Nature. 1992;355:318-25 pubmed
    The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single- and double-stranded DNA...
  26. Zaitsev E, Kowalczykowski S. Essential monomer-monomer contacts define the minimal length for the N-terminus of RecA protein. Mol Microbiol. 1998;29:1317-8 pubmed
  27. Drees J, Lusetti S, Chitteni Pattu S, Inman R, Cox M. A RecA filament capping mechanism for RecX protein. Mol Cell. 2004;15:789-98 pubmed
    The RecX protein is a potent inhibitor of RecA protein activities. RecX functions by specifically blocking the extension of RecA filaments. In vitro, this leads to a net disassembly of RecA protein from circular single-stranded DNA...
  28. Veaute X, Delmas S, Selva M, Jeusset J, Le Cam E, Matic I, et al. UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli. EMBO J. 2005;24:180-9 pubmed
    ..In addition to RecFOR, we provide evidence that RecA contributes to toxicity in the rep uvrD mutant...
  29. Bork J, Cox M, Inman R. The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA. EMBO J. 2001;20:7313-22 pubmed
    ..The RecOR-facilitated binding of RecA protein to single-stranded DNA (ssDNA) that is bound by single-stranded DNA-binding protein (SSB) is much faster if ..
  30. Brendel V, Brocchieri L, Sandler S, Clark A, Karlin S. Evolutionary comparisons of RecA-like proteins across all major kingdoms of living organisms. J Mol Evol. 1997;44:528-41 pubmed
    Protein sequences with similarities to Escherichia coli RecA were compared across the major kingdoms of eubacteria, archaebacteria, and eukaryotes...
  31. Venkatesh R, Ganesh N, Guhan N, Reddy M, Chandrasekhar T, Muniyappa K. RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination. Proc Natl Acad Sci U S A. 2002;99:12091-6 pubmed
    In many eubacteria, coexpression of recX with recA is essential for attenuation of the deleterious effects of recA overexpression; however, the molecular mechanism has remained enigmatic...
  32. Lusetti S, Hobbs M, Stohl E, Chitteni Pattu S, Inman R, Seifert H, et al. The RecF protein antagonizes RecX function via direct interaction. Mol Cell. 2006;21:41-50 pubmed
    The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX...
  33. Michel B, Ehrlich S, Uzest M. DNA double-strand breaks caused by replication arrest. EMBO J. 1997;16:430-8 pubmed
    ..In wild-type E. coli, linear DNA was detected in the absence of RecBC or of both RecA and RecD. This reveals the existence of a significant amount of spontaneous DSBs...
  34. Yasuda T, Morimatsu K, Horii T, Nagata T, Ohmori H. Inhibition of Escherichia coli RecA coprotease activities by DinI. EMBO J. 1998;17:3207-16 pubmed
    ..The initial step, self-cleavage of the LexA repressor, is promoted by the RecA protein which is activated upon binding to single-stranded DNA...