rpoS

Summary

Gene Symbol: rpoS
Description: RNA polymerase, sigma S (sigma 38) factor
Alias: ECK2736, JW5437, abrD, appR, csi2, dpeB, katF, nur, otsX, sigS
Species: Escherichia coli str. K-12 substr. MG1655
Products:     rpoS

Top Publications

  1. Wise A, Brems R, Ramakrishnan V, Villarejo M. Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S. J Bacteriol. 1996;178:2785-93 pubmed
    ..Our data suggest that DNA sequences in the -35 region function as part of a discriminator mechanism to shift transcription between E sigma D and E sigma S. ..
  2. Bougdour A, Wickner S, Gottesman S. Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli. Genes Dev. 2006;20:884-97 pubmed
    ..Our results reveal a novel mechanism of regulation of sigma(S) stability through the regulation of RssB activity and identify IraP as a member of a new class of regulators, the anti-adaptor proteins. ..
  3. Chang Y, Wang A, Cronan J. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol Microbiol. 1994;11:1019-28 pubmed
    ..We report that PoxB activity is dependent on a functional rpoS(katF) gene which encodes a sigma factor required to transcribe a number of stationary-phase-induced genes...
  4. Guillemet M, Moreau P. Fur-dependent detoxification of organic acids by rpoS mutants during prolonged incubation under aerobic, phosphate starvation conditions. J Bacteriol. 2008;190:5567-75 pubmed publisher
    ..We show in this work that rpoS-null mutants incubated in the absence of any amino acid survived during prolonged incubation under aerobic, P(i) ..
  5. Dong T, Kirchhof M, Schellhorn H. RpoS regulation of gene expression during exponential growth of Escherichia coli K12. Mol Genet Genomics. 2008;279:267-77 pubmed
    b>RpoS is a major regulator of genes required for adaptation to stationary phase in E. coli...
  6. Typas A, Becker G, Hengge R. The molecular basis of selective promoter activation by the sigmaS subunit of RNA polymerase. Mol Microbiol. 2007;63:1296-306 pubmed
    ..how the principle sigma factor in stationary phase and in stressful conditions in Escherichia coli, sigmaS (RpoS), can specifically target its large regulon in vivo, although it is known to recognize the same core promoter ..
  7. Becker G, Hengge Aronis R. What makes an Escherichia coli promoter sigma(S) dependent? Role of the -13/-14 nucleotide promoter positions and region 2.5 of sigma(S). Mol Microbiol. 2001;39:1153-65 pubmed
    ..5 in sigmaS in transcription initiation. Moreover, we propose a consensus sequence for a sigmaS-selective promoter and discuss its emergence and functional properties from an evolutionary point of view. ..
  8. Flores N, Escalante A, de Anda R, Báez Viveros J, Merino E, Franco B, et al. New insights into the role of sigma factor RpoS as revealed in escherichia coli strains lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system. J Mol Microbiol Biotechnol. 2008;14:176-92 pubmed
    It has been demonstrated that about 10% of the Escherichia coli genes are under direct or indirect control of RpoS. Therefore, Weber et al...
  9. Weber H, Polen T, Heuveling J, Wendisch V, Hengge R. Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity. J Bacteriol. 2005;187:1591-603 pubmed
    The sigmaS (or RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli...
  10. Ferenci T. What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli?. Trends Microbiol. 2003;11:457-61 pubmed
    Pathogenic and commensal Escherichia coli isolates frequently contain defective alleles of the mutS and rpoS genes, located in a highly polymorphic segment of the chromosome...

Detail Information

Publications62

  1. Wise A, Brems R, Ramakrishnan V, Villarejo M. Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S. J Bacteriol. 1996;178:2785-93 pubmed
    ..Our data suggest that DNA sequences in the -35 region function as part of a discriminator mechanism to shift transcription between E sigma D and E sigma S. ..
  2. Bougdour A, Wickner S, Gottesman S. Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli. Genes Dev. 2006;20:884-97 pubmed
    ..Our results reveal a novel mechanism of regulation of sigma(S) stability through the regulation of RssB activity and identify IraP as a member of a new class of regulators, the anti-adaptor proteins. ..
  3. Chang Y, Wang A, Cronan J. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol Microbiol. 1994;11:1019-28 pubmed
    ..We report that PoxB activity is dependent on a functional rpoS(katF) gene which encodes a sigma factor required to transcribe a number of stationary-phase-induced genes...
  4. Guillemet M, Moreau P. Fur-dependent detoxification of organic acids by rpoS mutants during prolonged incubation under aerobic, phosphate starvation conditions. J Bacteriol. 2008;190:5567-75 pubmed publisher
    ..We show in this work that rpoS-null mutants incubated in the absence of any amino acid survived during prolonged incubation under aerobic, P(i) ..
  5. Dong T, Kirchhof M, Schellhorn H. RpoS regulation of gene expression during exponential growth of Escherichia coli K12. Mol Genet Genomics. 2008;279:267-77 pubmed
    b>RpoS is a major regulator of genes required for adaptation to stationary phase in E. coli...
  6. Typas A, Becker G, Hengge R. The molecular basis of selective promoter activation by the sigmaS subunit of RNA polymerase. Mol Microbiol. 2007;63:1296-306 pubmed
    ..how the principle sigma factor in stationary phase and in stressful conditions in Escherichia coli, sigmaS (RpoS), can specifically target its large regulon in vivo, although it is known to recognize the same core promoter ..
  7. Becker G, Hengge Aronis R. What makes an Escherichia coli promoter sigma(S) dependent? Role of the -13/-14 nucleotide promoter positions and region 2.5 of sigma(S). Mol Microbiol. 2001;39:1153-65 pubmed
    ..5 in sigmaS in transcription initiation. Moreover, we propose a consensus sequence for a sigmaS-selective promoter and discuss its emergence and functional properties from an evolutionary point of view. ..
  8. Flores N, Escalante A, de Anda R, Báez Viveros J, Merino E, Franco B, et al. New insights into the role of sigma factor RpoS as revealed in escherichia coli strains lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system. J Mol Microbiol Biotechnol. 2008;14:176-92 pubmed
    It has been demonstrated that about 10% of the Escherichia coli genes are under direct or indirect control of RpoS. Therefore, Weber et al...
  9. Weber H, Polen T, Heuveling J, Wendisch V, Hengge R. Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity. J Bacteriol. 2005;187:1591-603 pubmed
    The sigmaS (or RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli...
  10. Ferenci T. What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli?. Trends Microbiol. 2003;11:457-61 pubmed
    Pathogenic and commensal Escherichia coli isolates frequently contain defective alleles of the mutS and rpoS genes, located in a highly polymorphic segment of the chromosome...
  11. Ruiz N, Silhavy T. Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion. J Bacteriol. 2003;185:5984-92 pubmed
    Regulation of the sigma factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether Escherichia coli turns on or off the stationary-phase response...
  12. Repoila F, Majdalani N, Gottesman S. Small non-coding RNAs, co-ordinators of adaptation processes in Escherichia coli: the RpoS paradigm. Mol Microbiol. 2003;48:855-61 pubmed
    ..This suggests that they play a role in cell adaptation. In this review, we use the regulation of RpoS (sigma38) translation as a paradigm of sRNA-mediated response to environmental conditions; rpoS is currently the ..
  13. Farewell A, Kvint K, Nystrom T. Negative regulation by RpoS: a case of sigma factor competition. Mol Microbiol. 1998;29:1039-51 pubmed
    A mutation in the Escherichia coli gene encoding the stationary phase-inducible sigma factor (sigmaS, RpoS) not only abolishes transcription of some genes in stationary phase, but also causes superinduction of other stationary phase-..
  14. Rajkumari K, Gowrishankar J. An N-terminally truncated RpoS (sigma(S)) protein in Escherichia coli is active in vivo and exhibits normal environmental regulation even in the absence of rpoS transcriptional and translational control signals. J Bacteriol. 2002;184:3167-75 pubmed
    b>RpoS (sigma(S)) in Escherichia coli is a stationary-phase-specific primary sigma factor of RNA polymerase which is 330 amino acids long and belongs to the eubacterial sigma(70) family of proteins. Conserved domain 1...
  15. Jishage M, Kvint K, Shingler V, Nystrom T. Regulation of sigma factor competition by the alarmone ppGpp. Genes Dev. 2002;16:1260-70 pubmed
    ..Thus, the stringent response encompasses a mechanism that alters the relative competitiveness of sigma factors in accordance with cellular demands during physiological stress. ..
  16. Becker G, Klauck E, Hengge Aronis R. Regulation of RpoS proteolysis in Escherichia coli: the response regulator RssB is a recognition factor that interacts with the turnover element in RpoS. Proc Natl Acad Sci U S A. 1999;96:6439-44 pubmed
    The degradation of the RpoS (sigmaS) subunit of RNA polymerase in Escherichia coli is a prime example of regulated proteolysis in prokaryotes...
  17. Merrikh H, Ferrazzoli A, Lovett S. Growth phase and (p)ppGpp control of IraD, a regulator of RpoS stability, in Escherichia coli. J Bacteriol. 2009;191:7436-46 pubmed publisher
    The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase...
  18. Resch A, Afonyushkin T, Lombo T, McDowall K, Blasi U, Kaberdin V. Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5'-leader. RNA. 2008;14:454-9 pubmed publisher
    The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigmaS, includes translational activation by the noncoding RNA DsrA...
  19. Igarashi K, Kashiwagi K. Polyamine Modulon in Escherichia coli: genes involved in the stimulation of cell growth by polyamines. J Biochem. 2006;139:11-6 pubmed
    ..protein (OppA), which is important for nutrient supply, adenylate cyclase (Cya), RNA polymerase sigma(38) subunit (RpoS), transcription factor of iron transport operon (FecI), and transcription factor of growth-related genes including ..
  20. Merrikh H, Ferrazzoli A, Bougdour A, Olivier Mason A, Lovett S. A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS. Proc Natl Acad Sci U S A. 2009;106:611-6 pubmed publisher
    ..are consistent with the function of IraD to promote accumulation of the alternative transcription sigma factor, RpoS, by binding to the adaptor RssB protein that targets RpoS for degradation...
  21. Bougdour A, Cunning C, Baptiste P, Elliott T, Gottesman S. Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors. Mol Microbiol. 2008;68:298-313 pubmed
    ..Our results reveal that multiple anti-adaptor proteins allow the regulation of sigmaS stability through the regulation of RssB activity under a variety of stress conditions...
  22. Majdalani N, Hernandez D, Gottesman S. Regulation and mode of action of the second small RNA activator of RpoS translation, RprA. Mol Microbiol. 2002;46:813-26 pubmed
    Translation of the stationary phase sigma factor RpoS is stimulated by at least two small RNAs, DsrA and RprA. DsrA disrupts an inhibitory secondary structure in the rpoS leader mRNA by pairing with the upstream RNA...
  23. Hengge Aronis R. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiol Mol Biol Rev. 2002;66:373-95, table of contents pubmed
    The sigma(S) (RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli and related bacteria...
  24. Vijayakumar S, Kirchhof M, Patten C, Schellhorn H. RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis. J Bacteriol. 2004;186:8499-507 pubmed
    b>RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized...
  25. Lacour S, Landini P. SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences. J Bacteriol. 2004;186:7186-95 pubmed
    The sigma(S) subunit of RNA polymerase, the product of the rpoS gene, controls the expression of genes responding to starvation and cellular stresses...
  26. Barth E, Gora K, Gebendorfer K, Settele F, Jakob U, Winter J. Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli. Microbiology. 2009;155:1680-9 pubmed publisher
    ..Downregulation of CRP in turn causes the derepression of rpoS, encoding the alternative sigma factor sigma(S), which activates the general stress response in E. coli...
  27. Gill R, Valdes J, Bentley W. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab Eng. 2000;2:178-89 pubmed
    ..ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage life cycle (ftsH, recA) genes...
  28. Subbarayan P, Sarkar M. A comparative study of variation in codon 33 of the rpoS gene in Escherichia coli K12 stocks: implications for the synthesis of sigma(s). Mol Genet Genomics. 2004;270:533-8 pubmed publisher
    The Escherichia coli rpoS gene encodes an RNA polymerase sigma factor (sigma S or sigma(S)) required for the expression of stationary-phase genes. In the first published rpoS sequence from E. coli K-12 codon 33 is given as CAG...
  29. Moreau P, Gerard F, Lutz N, Cozzone P. Non-growing Escherichia coli cells starved for glucose or phosphate use different mechanisms to survive oxidative stress. Mol Microbiol. 2001;39:1048-60 pubmed
    ..in an OxyR-dependent manner in response to H2O2 challenge, we found that the inactivation of oxyR and both oxyR and rpoS genes had little effect on the viability of Pi-starved cells...
  30. Schweder T, Lee K, Lomovskaya O, Matin A. Regulation of Escherichia coli starvation sigma factor (sigma s) by ClpXP protease. J Bacteriol. 1996;178:470-6 pubmed
    ....
  31. Zhou Y, Gottesman S. Modes of regulation of RpoS by H-NS. J Bacteriol. 2006;188:7022-5 pubmed
    Regulated degradation of RpoS requires RssB and ClpXP protease...
  32. Ishihama A. Protein-protein communication within the transcription apparatus. J Bacteriol. 1993;175:2483-9 pubmed
  33. Takayanagi Y, Tanaka K, Takahashi H. Structure of the 5' upstream region and the regulation of the rpoS gene of Escherichia coli. Mol Gen Genet. 1994;243:525-31 pubmed
    The nucleotide sequence of the 5' upstream region of the Escherichia coli rpoS gene was determined and analyzed...
  34. Bordes P, Conter A, Morales V, Bouvier J, Kolb A, Gutierrez C. DNA supercoiling contributes to disconnect sigmaS accumulation from sigmaS-dependent transcription in Escherichia coli. Mol Microbiol. 2003;48:561-71 pubmed
    ....
  35. Kolodkin Gal I, Engelberg Kulka H. The stationary-phase sigma factor sigma(S) is responsible for the resistance of Escherichia coli stationary-phase cells to mazEF-mediated cell death. J Bacteriol. 2009;191:3177-82 pubmed publisher
    ..Deletion of rpoS, the gene encoding sigma(S) from the bacterial chromosome, permitted mazEF-mediated cell death during stationary ..
  36. Ferrières L, Thompson A, Clarke D. Elevated levels of sigma S inhibit biofilm formation in Escherichia coli: a role for the Rcs phosphorelay. Microbiology. 2009;155:3544-53 pubmed publisher
    ..Therefore, the Rcs phosphorelay is involved in maintaining appropriate levels of sigma(S) during biofilm formation in E. coli. ..
  37. Rosenthal A, Hu M, Gralla J. Osmolyte-induced transcription: -35 region elements and recognition by sigma38 (rpoS). Mol Microbiol. 2006;59:1052-61 pubmed
    ..The ensuing transcriptional activation is mediated by unknown elements at sigma38 (rpoS)-dependent promoters...
  38. Moorthy S, Mahadevan S. Differential spectrum of mutations that activate the Escherichia coli bgl operon in an rpoS genetic background. J Bacteriol. 2002;184:4033-8 pubmed
    ..The rpoS-encoded sigmaS, the stationary-phase sigma factor, is involved in both physiological as well as genetic changes ..
  39. Muffler A, Fischer D, Hengge Aronis R. The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli. Genes Dev. 1996;10:1143-51 pubmed
    The rpoS-encoded sigma(S) subunit of RNA polymerase in Escherichia coli is a global regulatory factor involved in several stress responses...
  40. Joo D, Nolte A, Calendar R, Zhou Y, Jin D. Multiple regions on the Escherichia coli heat shock transcription factor sigma32 determine core RNA polymerase binding specificity. J Bacteriol. 1998;180:1095-102 pubmed
    ..2, and the other was in a region conserved only among heat shock sigma factors. These data suggest that there is more than one core RNAP binding region in sigma32 and that differences in contact sites probably exist among sigma factors. ..
  41. Kim E, Shin M, Rhee J, Choy H. Factors influencing preferential utilization of RNA polymerase containing sigma-38 in stationary-phase gene expression in Escherichia coli. J Microbiol. 2004;42:103-10 pubmed
    ..Thus, the preferential expression of stationary-phase genes by Esigma38 is unlikely the consequence of selective inhibition of Esigma70 by 6S RNA. ..
  42. Colland F, Fujita N, Kotlarz D, Bown J, Meares C, Ishihama A, et al. Positioning of sigma(S), the stationary phase sigma factor, in Escherichia coli RNA polymerase-promoter open complexes. EMBO J. 1999;18:4049-59 pubmed
    ..2 reacts only weakly with the -35 promoter element. This provides a structural basis for the minor role of the -35 sequence in sigma(S)-dependent promoter recognition. ..