DNApol-alpha180

Summary

Gene Symbol: DNApol-alpha180
Description: DNA polymerase alpha 180kD
Alias: 183 kDa pol alpha, AAB24152, CG6349, DNA Pol-alpha180, DNA polalpha, DNA polymerase alpha 180 kD, DNA-Polalpha, DNApol, DNApol-alpha, DNApola, DNApolalpha, DNApolalpha180, DmPolalpha, Dmel\CG6349, Dmpol alpha, E(mus304), POLA, POlalpha 180kDa, Pol, Pol alpha, Polalpha, dpolalphap180, pol alpha, polalpha, polalphap180, CG6349 gene product from transcript CG6349-RA, 180 kDa subunit, 180 kDa subunit of polymerase-primase, CG6349-PA, DNA pol 180, DNA polymerase 180K, DNA polymerase alpha, DNA polymerase alpha 148,000 alpha subunit, DNA polymerase alpha 180 kD, DNA polymerase alpha 180-kDa subunit, DNA polymerase alpha 182,000 alpha subunit, DNA polymerase-primase 180 kda subunit, DNA-polymerase alpha, DNApol-alpha180-PA, enhancer of mus304
Species: fruit fly

Top Publications

  1. pmc A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site
    J C Shen
    Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
    Nucleic Acids Res 20:5119-25. 1992
  2. ncbi Molecular characterisation of the gene for the 180 kDa subunit of the DNA polymerase-primase of Drosophila melanogaster
    S Melov
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    J Cell Sci 102:847-56. 1992
  3. ncbi Elongation of RNA-primed DNA templates by DNA polymerase alpha from Drosophila melanogaster embryos
    G Villani
    J Biol Chem 256:8202-7. 1981
  4. ncbi Novel 8-base pair sequence (Drosophila DNA replication-related element) and specific binding factor involved in the expression of Drosophila genes for DNA polymerase alpha and proliferating cell nuclear antigen
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    J Biol Chem 268:2092-9. 1993
  5. doi The Spartan ortholog maternal haploid is required for paternal chromosome integrity in the Drosophila zygote
    Laetitia Delabaere
    Centre de Génétique et de Physiologie Moléculaire et Cellulaire, CNRS UMR5534, Universite Claude Bernard Lyon 1, University of Lyon, Villeurbanne 69100, France
    Curr Biol 24:2281-7. 2014
  6. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
  7. pmc Sources and structures of mitotic crossovers that arise when BLM helicase is absent in Drosophila
    Matthew C LaFave
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599
    Genetics 196:107-18. 2014
  8. doi DNA polymerase α interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila
    Ritsuko Sahashi
    Department Basic Medical Sciences, St Georges University London, Cranmer Terrace, London SW17 0RE, UK Department of Applied Biology, Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Cell Sci 127:3066-78. 2014
  9. pmc A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes
    Hangnoh Lee
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, USA
    Mol Cell Biol 32:2110-20. 2012
  10. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012

Research Grants

  1. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2002

Scientific Experts

  • Y Mizushina
  • Irwin Davidson
  • PETER BURGERS
  • M H Brodsky
  • Osamu Suyari
  • F Hirose
  • M Yamaguchi
  • Laetitia Delabaere
  • Matthew C LaFave
  • Ritsuko Sahashi
  • Masamitsu Yamaguchi
  • Hangnoh Lee
  • Justin A Gosnell
  • Jeff Sekelsky
  • A Matsukage
  • Kengo Sakaguchi
  • Sabrina J Kugler
  • Jeannine R Larocque
  • K Kuroda
  • Joogyung Hyun
  • Ryo Takeuchi
  • Maxim V Frolov
  • Julie K Holsclaw
  • Mario Martinez Saura
  • Gilles Crevel
  • Lewis J Overton
  • Béatrice Horard
  • Sabrina L Andersen
  • Guillermo A Orsi
  • Kathryn P Kohl
  • Laure Sapey-Triomphe
  • Sue Cotterill
  • Pierre Couble
  • Jaroslaw Pasko
  • Benjamin Loppin
  • Eric P Stoffregen
  • Rika Nagai
  • G Crevel
  • Hideki Yoshida
  • Dessislava K Dimova
  • Jagdip Gill
  • Hiroyuki Ida
  • Linda Ragusano
  • G Bosco
  • Ana Martinez
  • Mieko Kawai
  • Tim W Christensen
  • P G Mitsis
  • T L Orr-Weaver
  • T Sasaki
  • Y Nishimoto
  • T Shinomiya
  • Anja C Nagel
  • S Cotterill
  • Diana L Dougherty
  • Sumreen K Hussain
  • I Royzman
  • Dirk Bohmann
  • Heinrich Jasper
  • I R Lehman
  • Tatsushi Ruike
  • Kaori Shimanouchi
  • Yoshihiro Kanai
  • Gen Ishikawa
  • Makiyo Uchida
  • Hiroshi Morioka
  • Kei ichi Takata
  • Masahiko Oshige
  • Nicholas J Dyson
  • Dessislava Dimova
  • Nam Sung Moon
  • Erick J Morris
  • Olivier Stevaux
  • Eun Jeong Kwon
  • V Leclerc
  • T Hachiya
  • W Du
  • H Bates
  • M Ikeda
  • H Huikeshoven
  • V M Peck
  • T Sawado
  • A Omori
  • R F Marton
  • R T Kamakaka
  • Y Hayashi
  • M F Goodman
  • E A Nigg
  • P H O'Farrell
  • J P Tassan

Detail Information

Publications52

  1. pmc A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site
    J C Shen
    Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
    Nucleic Acids Res 20:5119-25. 1992
    ..mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity...
  2. ncbi Molecular characterisation of the gene for the 180 kDa subunit of the DNA polymerase-primase of Drosophila melanogaster
    S Melov
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    J Cell Sci 102:847-56. 1992
    We have cloned and sequenced the gene for the 180 kDa subunit of the a polymerase from Drosophila melanogaster. The protein shows high similarity to the 180 kDa subunits from other species...
  3. ncbi Elongation of RNA-primed DNA templates by DNA polymerase alpha from Drosophila melanogaster embryos
    G Villani
    J Biol Chem 256:8202-7. 1981
  4. ncbi Novel 8-base pair sequence (Drosophila DNA replication-related element) and specific binding factor involved in the expression of Drosophila genes for DNA polymerase alpha and proliferating cell nuclear antigen
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    J Biol Chem 268:2092-9. 1993
    ..DNA replication-related element (DRE)), are required for the high expression of Drosophila genes for DNA polymerase alpha and the proliferating cell nuclear antigen (PCNA) (an auxiliary protein for DNA polymerase delta)...
  5. doi The Spartan ortholog maternal haploid is required for paternal chromosome integrity in the Drosophila zygote
    Laetitia Delabaere
    Centre de Génétique et de Physiologie Moléculaire et Cellulaire, CNRS UMR5534, Universite Claude Bernard Lyon 1, University of Lyon, Villeurbanne 69100, France
    Curr Biol 24:2281-7. 2014
    ....
  6. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
    ..A striking result was that while primers were extended past an abasic lesion by HIV-1 RT in both direct and misalignment modes, avian myeloblastosis virus RT failed to catalyze significant extension by either mode...
  7. pmc Sources and structures of mitotic crossovers that arise when BLM helicase is absent in Drosophila
    Matthew C LaFave
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599
    Genetics 196:107-18. 2014
    ..These findings provide important new insights into sources and structures of mitotic crossovers and functions of BLM helicase. ..
  8. doi DNA polymerase α interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila
    Ritsuko Sahashi
    Department Basic Medical Sciences, St Georges University London, Cranmer Terrace, London SW17 0RE, UK Department of Applied Biology, Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Cell Sci 127:3066-78. 2014
    ....
  9. pmc A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes
    Hangnoh Lee
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, USA
    Mol Cell Biol 32:2110-20. 2012
    ..First, it is required for the stability of the repressive dE2F2/RBF complexes at their promoters during S phase. Second, we find that dREAM is indispensable for both transcriptional repression mechanisms employed at these genes...
  10. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012
    ..carries well-conserved six DNA polymerase subdomains and five 3'→5' exonuclease motifs as observed with Polε in other species...
  11. pmc Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression
    Justin A Gosnell
    Department of Biology, East Carolina University, Greenville, NC 27858, USA
    BMC Mol Biol 12:13. 2011
    ..As such, understanding the role of Ctf4 within the context of a multicellular organism will be integral to our understanding of its potential role in developmental and disease processes...
  12. pmc Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
    ..Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta)...
  13. ncbi Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster
    P G Mitsis
    Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
    Methods Enzymol 262:62-77. 1995
  14. pmc Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II
    V Leclerc
    URA 671 Centre National de la Recherche Scientifique, Villefranche sur Mer, France
    Mol Biol Cell 7:505-13. 1996
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function...
  15. ncbi Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies
    M Yamaguchi
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Biol Cell 85:147-55. 1995
    ..Using these antibodies together with those for DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), we examined expression patterns and sub-cellular ..
  16. pmc Distinct roles of E2F recognition sites as positive or negative elements in regulation of the DNA polymerase alpha 180 kDa catalytic subunit gene promoter during Drosophila development
    M Yamaguchi
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464, Japan
    Nucleic Acids Res 25:3847-54. 1997
    ..GST)-E2F and GST-DP fusion proteins were found to cooperate in binding to the three E2F sites in the DNA polymerase alpha gene promoter in vitro...
  17. pmc Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster
    T Sasaki
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Mol Cell Biol 19:547-55. 1999
    ..In the DNA polymerase alpha gene (DNApolalpha) locus, where an initiation region, oriDalpha, had been identified in cultured Kc cells, ..
  18. ncbi S phase and differential DNA replication during Drosophila oogenesis
    I Royzman
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Genes Cells 3:767-76. 1998
    ..Cyclin E and E2F are needed for this differential DNA replication during Drosophila oogenesis...
  19. pmc Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, 464 8681, Japan
    Mol Cell Biol 19:6020-8. 1999
    ..of the N-terminal fragment in salivary gland cells reduced the contents of mRNAs for the 180-kDa subunit of DNA polymerase alpha and for dE2F and the extent of DNA endoreplication...
  20. pmc mus304 encodes a novel DNA damage checkpoint protein required during Drosophila development
    M H Brodsky
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 USA
    Genes Dev 14:666-78. 2000
    ..Similar mechanisms may account for the puzzling array of symptoms observed in humans with mutations in the ATM tumor suppressor gene...
  21. ncbi Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 271:3930-7. 1996
    ..The antibodies inhibited specifically the transcription of DNA polymerase alpha promoter in vitro...
  22. ncbi DNA replication control through interaction of E2F-RB and the origin recognition complex
    G Bosco
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 3:289-95. 2001
    ..Our results indicate that dE2F1 and Rbf function together at replication origins to limit DNA replication through interactions with DmORC...
  23. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001
    ..protein (DNA polymerase interacting tpr containing protein of 47 kDa) through its interaction with the DNA polymerase alpha. This protein is a predominantly nuclear protein, which forms a tight and stoichiometric interaction with ..
  24. ncbi Eukaryotic DNA polymerases: proposal for a revised nomenclature
    P M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:43487-90. 2001
  25. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression...
  26. ncbi Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi, Hyogo 651 2180, Japan
    Biochim Biophys Acta 1648:55-61. 2003
    ..In this paper, we report that this compound could selectively inhibit the activities of DNA polymerases (pol. alpha, beta, gamma, delta and epsilon ) only from species in the deuterostome branch in the animal kingdom, like ..
  27. ncbi The genetics of TBP and TBP-related factors
    Irwin Davidson
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 1 rue Laurent Fries, 67404 Illkirch Cedex, France
    Trends Biochem Sci 28:391-8. 2003
  28. pmc DREF is required for efficient growth and cell cycle progression in Drosophila imaginal discs
    Joogyung Hyun
    Department of Biomedical Genetics, The Aab Institute of Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 633, Rochester, NY 14642, USA
    Mol Cell Biol 25:5590-8. 2005
    ..These data establish DREF as a global regulator of transcriptional programs that mediate cell proliferation and organ growth during animal development...
  29. pmc Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects
    Jeannine R Larocque
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genetics 176:1441-51. 2007
    ..We found that E(mus304) is a mutation in DNApol-alpha180, which encodes the catalytic subunit of DNA polymerase alpha. We did not find any defects resulting from reducing Polalpha by itself...
  30. pmc putzig is required for cell proliferation and regulates notch activity in Drosophila
    Sabrina J Kugler
    Institute of Genetics 240, University of Hohenheim, 70599 Stuttgart, Germany
    Mol Biol Cell 18:3733-40. 2007
    ..Our findings uncover different modes of operation of pzg during imaginal development of Drosophila, and they provide a novel mechanism of Notch regulation...
  31. ncbi DNA polymerase alpha from Drosophila melanogaster embryos. Subunit structure
    G Villani
    J Biol Chem 255:9479-83. 1980
    The homogeneous DNA polymerase alpha from early embryos of Drosophila melanogaster contains four polypeptides designated alpha, beta, gamma, and delta, with molecular weights of 148,000, 58,000, 46,000, and 43,000, respectively (Banks, G...
  32. pmc Delta-type DNA polymerase characterized from Drosophila melanogaster embryos
    V M Peck
    Department of Radiation Oncology, Arizona Cancer Center, University of Arizona, Tucson 85724
    Nucleic Acids Res 20:5779-84. 1992
    ..Two types of DNA polymerase, DNA polymerase delta and DNA polymerase alpha, were distinguished by: 1. copurification of DNA primase or 3'-5'exonuclease activities; 2...
  33. ncbi Interaction of Drosophila DNA polymerase alpha holoenzyme with synthetic template-primers containing mismatched primer bases or propanodeoxyguanosine adducts at various positions in template and primer regions
    S J Weiss
    Department of Pharmacological Sciences, State University of New York, Stony Brook 11794 8651
    J Biol Chem 267:18520-6. 1992
    We studied recognition and binding of synthetic template-primers by Drosophila DNA polymerase alpha (pol alpha) holoenzyme...
  34. ncbi Distribution of DNA polymerase alpha during nuclear division cycles in Drosophila melanogaster embryo
    M Yamaguchi
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Cell Struct Funct 17:105-12. 1992
    An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization...
  35. pmc Structure and expression during development of Drosophila melanogaster gene for DNA polymerase alpha
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Research Institute, Nagoya, Japan
    Nucleic Acids Res 19:4991-8. 1991
    The Drosophila melanogaster gene and cDNA which span the entire open reading frame for DNA polymerase alpha, were cloned, and their nucleotide sequences were determined. The gene consists of 6 exons separated by 5 short introns...
  36. ncbi Comparative efficiency of forming m4T.G versus m4T.A base pairs at a unique site by use of Escherichia coli DNA polymerase I (Klenow fragment) and Drosophila melanogaster polymerase alpha-primase complex
    M K Dosanjh
    Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Biochemistry 29:4698-703. 1990
    ..coli DNA polymerase I (Klenow fragment, Kf) and Drosophila melanogaster polymerase alpha-primase complex (pol alpha) were used. On the basis of the Vmax/Km ratios, the pairing of m4T.G was preferred over that of both m4T.A and T...
  37. ncbi Immunoaffinity purification and properties of Drosophila melanogaster DNA polymerase alpha-primase complex
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo
    J Biochem 108:926-33. 1990
    Two hybrid cell lines (DM88-5E12 and DM88-4C9) secreting monoclonal antibodies against DNA polymerase alpha-primase complex from Drosophila melanogaster Kc cells were established by immunizing mice with the complex partially purified by a ..
  38. ncbi Purification and properties of an accessory protein for DNA polymerase alpha/primase
    M Goulian
    Department of Medicine, University of California, San Diego, La Jolla 92093 0613
    J Biol Chem 265:13221-30. 1990
    A protein that stimulates DNA polymerase alpha/primase many-fold on unprimed poly(dT) was purified to homogeneity from extracts of cultured mouse cells...
  39. ncbi Site-directed mutagenesis for quantitation of base-base interactions at defined sites
    B Singer
    Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Mutat Res 233:45-51. 1990
    ..All enzymes led to approximately the same frequency of transitions. It is postulated that the mutation frequency at a given site is primarily a function of the structure of the sequence around the target site...
  40. pmc Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster
    L S Kaguni
    Proc Natl Acad Sci U S A 80:2221-5. 1983
    A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000...
  41. ncbi Association of DNA primase with the beta/gamma subunits of DNA polymerase alpha from Drosophila melanogaster embryos
    L S Kaguni
    J Biol Chem 258:9037-9. 1983
    The DNA polymerase and primase activities of the intact DNA polymerase alpha from early embryos of Drosophila melanogaster co-sediment in native glycerol gradients. However, the activities are separated in glycerol gradients containing 2...
  42. ncbi A high molecular weight DNA polymerase from Drosophila melanogaster embryos. Purification, structure, and partial characterization
    G R Banks
    J Biol Chem 254:9886-92. 1979
    ..It was inhibited by low concentrations of N-ethylmaleimide and Aphidicolon. Thus the DNA polymerase of D. melanogaster resembles most closely the alpha-DNA polymerases that have been purified from mammalian cells...
  43. ncbi Immunological comparison of purified DNA polymerase alpha from embryos of Drosophila melanogaster with forms of the enzyme present in vivo
    B Sauer
    J Biol Chem 257:12394-8. 1982
    Specific antisera to purified DNA polymerase alpha from embryos of Drosophila melanogaster and to two of the four constituent subunits (alpha, beta, gamma, and delta) were prepared...
  44. ncbi A 130 kDa polypeptide immunologically related to the 180 kDa catalytic subunit of DNA polymerase alpha-primase complex is detected in early embryos of Drosophila
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo
    J Biochem 117:809-18. 1995
    An immunocytochemical method using specific antibodies was employed to detect DNA polymerase alpha-primase complex in Drosophila melanogaster embryos during the first 13 nuclear division cycles...
  45. ncbi Repression of regulatory factor for Drosophila DNA replication-related gene promoters by zerknüllt homeodomain protein
    F Hirose
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    J Biol Chem 269:2937-42. 1994
    ..to +45 nucleotide positions with respect to the major transcription initiation site) of the Drosophila DNA polymerase alpha gene was repressed by cotransfection into Drosophila Kc cells with a zerknüllt (zen)-expressing plasmid as ..
  46. pmc Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone
    T Shinomiya
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Mol Cell Biol 14:7394-403. 1994
    ..The initiation region was first localized downstream of the DNA polymerase alpha gene by determining direction of replication forks with the neutral/alkaline 2D gel method...
  47. pmc Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro
    R T Kamakaka
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0347
    Mol Cell Biol 14:5114-22. 1994
    ..Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction...
  48. ncbi Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster
    R F Marton
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    FEBS Lett 342:139-44. 1994
    ..We also demonstrate that dRP-A can stimulate the activity of its homologous DNA polymerase alpha in excess of 20 fold...
  49. ncbi A DNA polymerase alpha-associated 56 kDa protein kinase
    V M Peck
    Department of Radiation Oncology, University of Arizona Health Sciences Center, University of Arizona, Tucson 85724
    Biochem Biophys Res Commun 190:325-31. 1993
    ..We report that Drosophila embryo DNA polymerase alpha complex, isolated by immunological techniques, contains a protein kinase activity...
  50. ncbi Identification of the specific labile sites in the 180 kDa catalytic polypeptide of the Drosophila melanogaster DNA polymerase alpha-primase complex
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo
    J Biochem 113:126-8. 1993
    The immunoaffinity-purified DNA polymerase alpha-primase complex from Drosophila melanogaster Kc cells contains three high molecular weight polypeptides besides the 180 kDa catalytic polypeptide...
  51. ncbi A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA
    P G Mitsis
    Department of Biochemistry, Beckman Center, Stanford University School of Medicine, California 94305
    Biochemistry 32:5257-66. 1993
    ..D-SSB stimulates the extent and processivity of DNA synthesis of its cognate DNA polymerase alpha. On the basis of these properties, we conclude that D-SSB is the Drosophila cognate of the human and yeast ..

Research Grants1

  1. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2002
    The main goal of the research is to study the structure and function of DNA polymerase delta (Pol-delta), the principal DNA polymerase in the cell for DNA replication and for DNA repair...