DNApol-alpha50

Summary

Gene Symbol: DNApol-alpha50
Description: DNA polymerase alpha 50kD
Alias: CG7108, DNA polalpha, DNApol-alpha, DNApolalpha, DNApolalpha-50, DNApolalpha50, Dmel\CG7108, Dmpol alpha, Dpri50, POLA, PRI1, Pol, Polalpha, Polalpha 50kDa, anon-WO0140519.257, dnapol alpha 50, pol alpha, polalpha, polalpha50, CG7108 gene product from transcript CG7108-RA, CG7108-PA, DNA polymerase 50K, DNA polymerase alpha, DNA polymerase alpha 46,000 gamma subunit, DNA polymerase alpha 50 kda primase subunit, DNA polymerase alpha 50,000 gamma subunit, DNA-polymerase alpha, DNApol-alpha50-PA, p50 subunit of DNA polymerase alpha
Species: fruit fly

Top Publications

  1. pmc Genome-Wide Analysis of Drosophila RBf2 Protein Highlights the Diversity of RB Family Targets and Possible Role in Regulation of Ribosome Biosynthesis
    Yiliang Wei
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1319
    G3 (Bethesda) 5:1503-15. 2015
  2. pmc Evidence for autoregulation and cell signaling pathway regulation from genome-wide binding of the Drosophila retinoblastoma protein
    Pankaj Acharya
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824 1319, USA
    G3 (Bethesda) 2:1459-72. 2012
  3. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
  4. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012
  5. pmc Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
  6. ncbi Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster
    P G Mitsis
    Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
    Methods Enzymol 262:62-77. 1995
  7. ncbi The Drosophila melanogaster UMP-CMP kinase cDNA encodes an N-terminal mitochondrial import signal
    Sophie Curbo
    Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, S 14186 Stockholm, Sweden
    Biochem Biophys Res Commun 311:440-5. 2003
  8. ncbi Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi, Hyogo 651 2180, Japan
    Biochim Biophys Acta 1648:55-61. 2003
  9. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003
  10. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001

Scientific Experts

  • Y Mizushina
  • Sophie Curbo
  • Yiliang Wei
  • R William Henry
  • David N Arnosti
  • Pankaj Acharya
  • Osamu Suyari
  • Kengo Sakaguchi
  • Ryo Takeuchi
  • Maxim V Frolov
  • Rima Mouawad
  • Bartek Wilczynski
  • Shamba S Mondal
  • Mieko Kawai
  • Nicolas Negre
  • Kevin P White
  • Hideki Yoshida
  • John Johnston
  • G Crevel
  • Masamitsu Yamaguchi
  • Hiroyuki Ida
  • P G Mitsis
  • S Cotterill
  • I Royzman
  • Gen Ishikawa
  • Hiroshi Morioka
  • Kaori Shimanouchi
  • Masahiko Oshige
  • Kei ichi Takata
  • Tatsushi Ruike
  • Yoshihiro Kanai
  • Makiyo Uchida
  • Eun Jeong Kwon
  • Erick J Morris
  • Nicholas J Dyson
  • Dessislava Dimova
  • Olivier Stevaux
  • Nam Sung Moon
  • V Leclerc
  • H Huikeshoven
  • H Bates
  • I R Lehman
  • R F Marton
  • R T Kamakaka
  • T L Orr-Weaver
  • C J Bakkenist
  • H Cai
  • E A Nigg
  • P H O'Farrell
  • M F Goodman
  • P Leopold
  • J P Tassan
  • C S Chiang
  • P Thommes
  • P D Kaufman
  • J C Shen
  • J T Kadonaga
  • B Stillman
  • L B Bloom
  • B Singer
  • R Eritja
  • M K Dosanjh
  • S C Kowalczykowski
  • G Villani
  • L S Kaguni
  • P A Jones
  • S Creighton
  • R C Conaway
  • J M Essigmann
  • J M Rossignol
  • B Sauer

Detail Information

Publications21

  1. pmc Genome-Wide Analysis of Drosophila RBf2 Protein Highlights the Diversity of RB Family Targets and Possible Role in Regulation of Ribosome Biosynthesis
    Yiliang Wei
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1319
    G3 (Bethesda) 5:1503-15. 2015
    ..The presence of unique RBf2 targets suggest that evolutionary appearance of this corepressor represents the acquisition of potentially novel roles in gene regulation for the RB family. ..
  2. pmc Evidence for autoregulation and cell signaling pathway regulation from genome-wide binding of the Drosophila retinoblastoma protein
    Pankaj Acharya
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824 1319, USA
    G3 (Bethesda) 2:1459-72. 2012
    ..Many of these targeted genes are bound by Rbf1 homologs in human cells, indicating that a conserved role of RB proteins may be to adjust the set point of interlinked signaling networks essential for growth and development...
  3. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
    ..A striking result was that while primers were extended past an abasic lesion by HIV-1 RT in both direct and misalignment modes, avian myeloblastosis virus RT failed to catalyze significant extension by either mode...
  4. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012
    ..carries well-conserved six DNA polymerase subdomains and five 3'→5' exonuclease motifs as observed with Polε in other species...
  5. pmc Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
    ..Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta)...
  6. ncbi Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster
    P G Mitsis
    Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
    Methods Enzymol 262:62-77. 1995
  7. ncbi The Drosophila melanogaster UMP-CMP kinase cDNA encodes an N-terminal mitochondrial import signal
    Sophie Curbo
    Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, S 14186 Stockholm, Sweden
    Biochem Biophys Res Commun 311:440-5. 2003
    ..This is the first time a mitochondrial pyrimidine nucleoside monophosphate kinase has been cloned from any organism and we discuss the implication of this finding for deoxyribonucleoside salvage in both Drosophila and other organisms...
  8. ncbi Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi, Hyogo 651 2180, Japan
    Biochim Biophys Acta 1648:55-61. 2003
    ..In this paper, we report that this compound could selectively inhibit the activities of DNA polymerases (pol. alpha, beta, gamma, delta and epsilon ) only from species in the deuterostome branch in the animal kingdom, like ..
  9. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression...
  10. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001
    ..protein (DNA polymerase interacting tpr containing protein of 47 kDa) through its interaction with the DNA polymerase alpha. This protein is a predominantly nuclear protein, which forms a tight and stoichiometric interaction with ..
  11. ncbi S phase and differential DNA replication during Drosophila oogenesis
    I Royzman
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Genes Cells 3:767-76. 1998
    ..Cyclin E and E2F are needed for this differential DNA replication during Drosophila oogenesis...
  12. pmc Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II
    V Leclerc
    URA 671 Centre National de la Recherche Scientifique, Villefranche sur Mer, France
    Mol Biol Cell 7:505-13. 1996
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function...
  13. pmc A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site
    J C Shen
    Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
    Nucleic Acids Res 20:5119-25. 1992
    ..mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity...
  14. ncbi A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA
    P G Mitsis
    Department of Biochemistry, Beckman Center, Stanford University School of Medicine, California 94305
    Biochemistry 32:5257-66. 1993
    ..D-SSB stimulates the extent and processivity of DNA synthesis of its cognate DNA polymerase alpha. On the basis of these properties, we conclude that D-SSB is the Drosophila cognate of the human and yeast ..
  15. ncbi Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster
    R F Marton
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    FEBS Lett 342:139-44. 1994
    ..We also demonstrate that dRP-A can stimulate the activity of its homologous DNA polymerase alpha in excess of 20 fold...
  16. pmc Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro
    R T Kamakaka
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0347
    Mol Cell Biol 14:5114-22. 1994
    ..Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction...
  17. ncbi The 50-kDa primase subunit of Drosophila melanogaster DNA polymerase alpha. Molecular characterization of the gene and functional analysis of the overexpressed protein
    C J Bakkenist
    Marie Curie Research Institute, Oxted, Surrey, United Kingdom
    J Biol Chem 269:26759-66. 1994
    The gene encoding the 50-kDa subunit of Drosophila melanogaster DNA polymerase alpha has been cloned...
  18. ncbi DNA polymerase alpha from Drosophila melanogaster embryos. Subunit structure
    G Villani
    J Biol Chem 255:9479-83. 1980
    The homogeneous DNA polymerase alpha from early embryos of Drosophila melanogaster contains four polypeptides designated alpha, beta, gamma, and delta, with molecular weights of 148,000, 58,000, 46,000, and 43,000, respectively (Banks, G...
  19. pmc Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster
    L S Kaguni
    Proc Natl Acad Sci U S A 80:2221-5. 1983
    A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000...
  20. ncbi Site-directed mutagenesis for quantitation of base-base interactions at defined sites
    B Singer
    Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Mutat Res 233:45-51. 1990
    ..All enzymes led to approximately the same frequency of transitions. It is postulated that the mutation frequency at a given site is primarily a function of the structure of the sequence around the target site...
  21. ncbi Comparative efficiency of forming m4T.G versus m4T.A base pairs at a unique site by use of Escherichia coli DNA polymerase I (Klenow fragment) and Drosophila melanogaster polymerase alpha-primase complex
    M K Dosanjh
    Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Biochemistry 29:4698-703. 1990
    ..coli DNA polymerase I (Klenow fragment, Kf) and Drosophila melanogaster polymerase alpha-primase complex (pol alpha) were used. On the basis of the Vmax/Km ratios, the pairing of m4T.G was preferred over that of both m4T.A and T...