DNApol-alpha73

Summary

Gene Symbol: DNApol-alpha73
Description: DNA polymerase alpha 73kD
Alias: CG5923, DNA pol-73, DNA polalpha, DNA polymerase alpha 73 kD, DNApol-alpha, DNApol-alpha72, DNApolalpha, Dmel\CG5923, Dmpol alpha, POLA, Pol, Polalpha, Polalpha 73kDa, pol alpha, polalpha, B subunit, CG5923-PC, DNA polymerase 73K, DNA polymerase alpha, DNA polymerase alpha primase 73 kda subunit, DNA-polymerase alpha, DNApol-alpha73-PC
Species: fruit fly

Top Publications

  1. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012
  2. pmc Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
  3. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
  4. pmc Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster
    T Sasaki
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Mol Cell Biol 19:547-55. 1999
  5. ncbi Phosphorylation and dephosphorylation of the B subunit of DNA polymerase alpha-primase complex in the early embryogenesis of Drosophila
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Machida, 194 8511, Japan
    Biochem Biophys Res Commun 254:372-7. 1999
  6. ncbi S phase and differential DNA replication during Drosophila oogenesis
    I Royzman
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Genes Cells 3:767-76. 1998
  7. ncbi Control of endoreduplication domains in the Drosophila gut by the knirps and knirps-related genes
    B Fuss
    Institut fur Zoologie, Universitat Bonn, Abteilung Entwicklungsbiologie, Romerstrasse 164, D 53117 Bonn, Germany
    Mech Dev 100:15-23. 2001
  8. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001
  9. ncbi The Drosophila maternal gene sésame is required for sperm chromatin remodeling at fertilization
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon, Villeurbanne, France
    Chromosoma 110:430-40. 2001
  10. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003

Scientific Experts

  • Y Mizushina
  • B Loppin
  • Osamu Suyari
  • Kengo Sakaguchi
  • K Kuroda
  • Ryo Takeuchi
  • Maxim V Frolov
  • M Yamaguchi
  • S Cotterill
  • Hiroyuki Ida
  • B Fuss
  • Masamitsu Yamaguchi
  • G Crevel
  • Hideki Yoshida
  • Mieko Kawai
  • P G Mitsis
  • T Sasaki
  • I Royzman
  • Masahiko Oshige
  • Kei ichi Takata
  • Gen Ishikawa
  • Tatsushi Ruike
  • Yoshihiro Kanai
  • Makiyo Uchida
  • Hiroshi Morioka
  • Kaori Shimanouchi
  • R Ueda
  • Eun Jeong Kwon
  • Erick J Morris
  • Dessislava Dimova
  • Nicholas J Dyson
  • Nam Sung Moon
  • Olivier Stevaux
  • V Leclerc
  • Y Takahashi
  • H Bates
  • C Lehmann
  • F Hirose
  • T Meissner
  • F Eckardt
  • H Huikeshoven
  • A Matsukage
  • M Hoch
  • R Bauer
  • T L Orr-Weaver
  • I R Lehman
  • T Sawado
  • T Shinomiya
  • R F Marton
  • R T Kamakaka
  • J Kobayashi
  • S Miyajima
  • J P Tassan
  • M F Goodman
  • A V Smith
  • H Cai
  • P Leopold
  • E A Nigg
  • P H O'Farrell
  • T Naruge
  • T Hachiya
  • K Tamai
  • Y Nishimoto
  • M Ikeda
  • C S Chiang
  • J C Shen
  • P Thommes
  • P D Kaufman
  • J T Kadonaga
  • B Stillman
  • L B Bloom
  • M K Dosanjh
  • J A King
  • B Singer
  • R Eritja
  • S C Kowalczykowski
  • S Creighton
  • P A Jones
  • P McLachlan
  • J M Essigmann

Detail Information

Publications24

  1. doi Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle
    Osamu Suyari
    Department of Applied Biology, Kyoto Institute of Technology, Sakyo ku, Kyoto 606 8585, Japan
    Gene 495:104-14. 2012
    ..carries well-conserved six DNA polymerase subdomains and five 3'→5' exonuclease motifs as observed with Polε in other species...
  2. pmc Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
    ..Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta)...
  3. ncbi Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase
    H Cai
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340
    J Biol Chem 268:23567-72. 1993
    ..A striking result was that while primers were extended past an abasic lesion by HIV-1 RT in both direct and misalignment modes, avian myeloblastosis virus RT failed to catalyze significant extension by either mode...
  4. pmc Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster
    T Sasaki
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Mol Cell Biol 19:547-55. 1999
    ..In the DNA polymerase alpha gene (DNApolalpha) locus, where an initiation region, oriDalpha, had been identified in cultured Kc cells, ..
  5. ncbi Phosphorylation and dephosphorylation of the B subunit of DNA polymerase alpha-primase complex in the early embryogenesis of Drosophila
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Machida, 194 8511, Japan
    Biochem Biophys Res Commun 254:372-7. 1999
    In the early embryos of Drosophila, the B subunit of the DNA polymerase alpha-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS-polyacrylamide gel electrophoresis...
  6. ncbi S phase and differential DNA replication during Drosophila oogenesis
    I Royzman
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Genes Cells 3:767-76. 1998
    ..Cyclin E and E2F are needed for this differential DNA replication during Drosophila oogenesis...
  7. ncbi Control of endoreduplication domains in the Drosophila gut by the knirps and knirps-related genes
    B Fuss
    Institut fur Zoologie, Universitat Bonn, Abteilung Entwicklungsbiologie, Romerstrasse 164, D 53117 Bonn, Germany
    Mech Dev 100:15-23. 2001
    ..Our results provide a novel link between morphogen-dependent positional information and the spatio-temporal regulation of cell cycle activity in the gut...
  8. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001
    ..protein (DNA polymerase interacting tpr containing protein of 47 kDa) through its interaction with the DNA polymerase alpha. This protein is a predominantly nuclear protein, which forms a tight and stoichiometric interaction with ..
  9. ncbi The Drosophila maternal gene sésame is required for sperm chromatin remodeling at fertilization
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon, Villeurbanne, France
    Chromosoma 110:430-40. 2001
    ..The abnormal chromatin organization of the male pronucleus does not prevent the formation of a male pronuclear envelope, which breaks down and reassembles in synchrony with maternally derived nuclei present in the same cytoplasm...
  10. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression...
  11. ncbi Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi, Hyogo 651 2180, Japan
    Biochim Biophys Acta 1648:55-61. 2003
    ..In this paper, we report that this compound could selectively inhibit the activities of DNA polymerases (pol. alpha, beta, gamma, delta and epsilon ) only from species in the deuterostome branch in the animal kingdom, like ..
  12. ncbi Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies
    M Yamaguchi
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Biol Cell 85:147-55. 1995
    ..Using these antibodies together with those for DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), we examined expression patterns and sub-cellular ..
  13. pmc Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II
    V Leclerc
    URA 671 Centre National de la Recherche Scientifique, Villefranche sur Mer, France
    Mol Biol Cell 7:505-13. 1996
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function...
  14. ncbi DNA replication-related elements cooperate to enhance promoter activity of the drosophila DNA polymerase alpha 73-kDa subunit gene
    Y Takahashi
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 271:14541-7. 1996
    An analysis was carried out on the promoter region of the Drosophila DNA polymerase alpha 73-kDa subunit gene and the factor(s) activating the promoter...
  15. pmc Cloning of the gene for the 73 kD subunit of the DNA polymerase alpha primase of Drosophila melanogaster
    S Cotterill
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Nucleic Acids Res 20:4325-30. 1992
    We have isolated both cDNA and genomic clones for the 73 kDa subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Analysis of these clones has identified an open reading frame of 1959 bases coding for a protein of 72...
  16. ncbi Comparative efficiency of forming m4T.G versus m4T.A base pairs at a unique site by use of Escherichia coli DNA polymerase I (Klenow fragment) and Drosophila melanogaster polymerase alpha-primase complex
    M K Dosanjh
    Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Biochemistry 29:4698-703. 1990
    ..coli DNA polymerase I (Klenow fragment, Kf) and Drosophila melanogaster polymerase alpha-primase complex (pol alpha) were used. On the basis of the Vmax/Km ratios, the pairing of m4T.G was preferred over that of both m4T.A and T...
  17. ncbi Site-directed mutagenesis for quantitation of base-base interactions at defined sites
    B Singer
    Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Mutat Res 233:45-51. 1990
    ..All enzymes led to approximately the same frequency of transitions. It is postulated that the mutation frequency at a given site is primarily a function of the structure of the sequence around the target site...
  18. ncbi A 130 kDa polypeptide immunologically related to the 180 kDa catalytic subunit of DNA polymerase alpha-primase complex is detected in early embryos of Drosophila
    K Kuroda
    Mitsubishi Kasei Institute of Life Sciences, Tokyo
    J Biochem 117:809-18. 1995
    An immunocytochemical method using specific antibodies was employed to detect DNA polymerase alpha-primase complex in Drosophila melanogaster embryos during the first 13 nuclear division cycles...
  19. pmc Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro
    R T Kamakaka
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0347
    Mol Cell Biol 14:5114-22. 1994
    ..Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction...
  20. ncbi Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster
    R F Marton
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    FEBS Lett 342:139-44. 1994
    ..We also demonstrate that dRP-A can stimulate the activity of its homologous DNA polymerase alpha in excess of 20 fold...
  21. pmc Identification of genomic regions required for DNA replication during Drosophila embryogenesis
    A V Smith
    Whitehead Institute, Massachusetts Institute of Technology, Cambridge 02142
    Genetics 135:817-29. 1993
    ..Additionally, our analysis identified a gene, pimples, that is required for the proper completion of mitosis in the post-blastoderm divisions of the embryo...
  22. ncbi A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA
    P G Mitsis
    Department of Biochemistry, Beckman Center, Stanford University School of Medicine, California 94305
    Biochemistry 32:5257-66. 1993
    ..D-SSB stimulates the extent and processivity of DNA synthesis of its cognate DNA polymerase alpha. On the basis of these properties, we conclude that D-SSB is the Drosophila cognate of the human and yeast ..
  23. ncbi Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster
    P G Mitsis
    Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
    Methods Enzymol 262:62-77. 1995
  24. pmc A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site
    J C Shen
    Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
    Nucleic Acids Res 20:5119-25. 1992
    ..mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity...