insc

Summary

Gene Symbol: insc
Description: inscuteable
Alias: 25/17, CG11312, Dmel\CG11312, INSC, Ins, Insc, fam, l(2)05475, l(2)k12405, nem, CG11312 gene product from transcript CG11312-RA, CG11312-PA, fata morgana, insc-PA, inscrutable, inscutable, not enough muscles
Species: fruit fly

Top Publications

  1. ncbi inscuteable, a neural precursor gene of Drosophila, encodes a candidate for a cytoskeleton adaptor protein
    R Kraut
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Cologne, Germany
    Dev Biol 174:65-81. 1996
  2. ncbi Role of inscuteable in orienting asymmetric cell divisions in Drosophila
    R Kraut
    Institute of Molecular and Cell Biology, National University of Singapore
    Nature 383:50-5. 1996
  3. ncbi Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila
    C P Shen
    Howard Hughes Medical Institute and Department of Physiology, University of California at San Francisco, 94143 0724, USA
    Cell 90:449-58. 1997
  4. pmc Binary sibling neuronal cell fate decisions in the Drosophila embryonic central nervous system are nonstochastic and require inscuteable-mediated asymmetry of ganglion mother cells
    M Buescher
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Genes Dev 12:1858-70. 1998
  5. ncbi Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143 0725, USA
    Cell 95:225-35. 1998
  6. ncbi Asymmetric cell division: from A to Z
    N Hawkins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genes Dev 12:3625-38. 1998
  7. ncbi A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions
    M Tio
    Institute of Molecular and Cell Biology, National University of Singapore Campus, Singapore 117609
    J Cell Sci 112:1541-51. 1999
  8. ncbi Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
    A Wodarz
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, Germany
    Nature 402:544-7. 1999
  9. ncbi Bazooka recruits Inscuteable to orient asymmetric cell divisions in Drosophila neuroblasts
    M Schober
    Research Institute of Molecular Pathology, Vienna, Austria
    Nature 402:548-51. 1999
  10. ncbi Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous system
    J A Kaltschmidt
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 2:7-12. 2000

Research Grants

  1. REGULATION OF NEUROGENESIS IN THE DROSOPHILA CNS
    Krishna Bhat; Fiscal Year: 2002
  2. GENETIC ANALYSIS OF PREHAIR MORPHOGENESIS
    Paul Adler; Fiscal Year: 2005
  3. DROSOPHILA SENSORY NEURON DEVELOPMENT
    Volker Hartenstein; Fiscal Year: 2006
  4. Niemann Pick C disease in Drosophila
    Krishna Bhat; Fiscal Year: 2007
  5. DROSOPHILA SENSORY NEURON DEVELOPMENT
    Volker Hartenstein; Fiscal Year: 2007
  6. Patterning of the Primitive Heart Tube in Zebrafish
    Jau Nian Chen; Fiscal Year: 2010
  7. Genetic Control of Intestinal Stem Cells in the Drosophila Hindgut
    Volker Hartenstein; Fiscal Year: 2010
  8. REGIONAL PATTERNING IN THE VENTRAL TELENCEPHALON
    GORDON J FISHELL; Fiscal Year: 2010
  9. MOLECULAR GENETIC ANALYSIS OF ASYMMETRIC CELL DIVISIONS
    Chris Doe; Fiscal Year: 2002
  10. Organogenesis Screen Using Tissue-Specific Zebrafish
    Jau Nian Chen; Fiscal Year: 2005

Scientific Experts

Detail Information

Publications126 found, 100 shown here

  1. ncbi inscuteable, a neural precursor gene of Drosophila, encodes a candidate for a cytoskeleton adaptor protein
    R Kraut
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Cologne, Germany
    Dev Biol 174:65-81. 1996
    ..The inscuteable mutant phenotype, together with these other observations, suggests a possible role for the protein in cytoskeleton organization...
  2. ncbi Role of inscuteable in orienting asymmetric cell divisions in Drosophila
    R Kraut
    Institute of Molecular and Cell Biology, National University of Singapore
    Nature 383:50-5. 1996
    ..The Inscuteable protein localizes to the apical cell cortex before mitosis, suggesting that Inscuteable functions in establishing polarity for asymmetric cell division...
  3. ncbi Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila
    C P Shen
    Howard Hughes Medical Institute and Department of Physiology, University of California at San Francisco, 94143 0724, USA
    Cell 90:449-58. 1997
    ..Our results suggest that miranda functions downstream of inscuteable and works as an adapter that connects Prospero to the basal cell membrane during asymmetric cell division...
  4. pmc Binary sibling neuronal cell fate decisions in the Drosophila embryonic central nervous system are nonstochastic and require inscuteable-mediated asymmetry of ganglion mother cells
    M Buescher
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Genes Dev 12:1858-70. 1998
    ..The correct positioning of Numb and the proper orientation of division require Inscuteable (Insc). Loss of insc results in the generation of equivalent sibling cells...
  5. ncbi Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143 0725, USA
    Cell 95:225-35. 1998
    ..We propose that PON is one component of a multimolecular machinery that localizes Numb by responding to polarity cues conserved in neural precursors and epithelial cells...
  6. ncbi Asymmetric cell division: from A to Z
    N Hawkins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genes Dev 12:3625-38. 1998
  7. ncbi A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions
    M Tio
    Institute of Molecular and Cell Biology, National University of Singapore Campus, Singapore 117609
    J Cell Sci 112:1541-51. 1999
    ..Finally, we show that although inscuteable RNA is normally apically localised, RNA localisation is not required for protein localisation or any aspects of inscuteable function...
  8. ncbi Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
    A Wodarz
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, Germany
    Nature 402:544-7. 1999
    ..As Bazooka is also responsible for the maintenance of apical-basal polarity in epithelial tissues, it may be the missing link between epithelial polarity and neuroblast polarity...
  9. ncbi Bazooka recruits Inscuteable to orient asymmetric cell divisions in Drosophila neuroblasts
    M Schober
    Research Institute of Molecular Pathology, Vienna, Austria
    Nature 402:548-51. 1999
    ..Our results suggest that direct binding to Bazooka mediates the asymmetric localization of Inscuteable and connects the asymmetric division of neuroblasts to the axis of epithelial apical-basal polarity...
  10. ncbi Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous system
    J A Kaltschmidt
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 2:7-12. 2000
    ..This observation contradicts the widely held hypothesis that the cleavage furrow is always placed midway between the two centrosomes...
  11. ncbi Modes of protein movement that lead to the asymmetric localization of partner of Numb during Drosophila neuroblast division
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143, USA
    Mol Cell 4:883-91. 1999
    ..This movement can occur when myosin motor activity is inhibited. However, the restriction of Pon to the basal cortex requires both actomyosin and Inscuteable...
  12. ncbi Asymmetric division of Drosophila neural stem cells: a basis for neural diversity
    F Matsuzaki
    CREST, Japan Science and Technology Corporation, Department of Developmental Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Aoba ku, 980 8575, Japan tohoku ac jp
    Curr Opin Neurobiol 10:38-44. 2000
    ..Our understanding now extends to the molecular nature of the cell polarity that underlies asymmetric divisions. This polarity is conserved among neural stem cells, epithelial cells and fertilized eggs...
  13. ncbi A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila
    M Schaefer
    Research Institute of Molecular Pathology IMP, Vienna, A 1030, Austria
    Curr Biol 10:353-62. 2000
    ..How apically localised Inscuteable directs asymmetric cell divisions is unclear...
  14. ncbi Rapsynoid/partner of inscuteable controls asymmetric division of larval neuroblasts in Drosophila
    M L Parmentier
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    J Neurosci 20:RC84. 2000
    ..neuroblasts, the asymmetric localization of cell fate determinants is under the control of the protein Inscuteable (Insc), which is itself localized asymmetrically as an apical crescent...
  15. pmc Drosophila atypical protein kinase C associates with Bazooka and controls polarity of epithelia and neuroblasts
    A Wodarz
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, 40225 Dusseldorf, Germany
    J Cell Biol 150:1361-74. 2000
    ..This study is the first functional analysis of an atypical protein kinase C isoform using a loss-of-function allele in a genetically tractable organism...
  16. ncbi Role of cortical tumour-suppressor proteins in asymmetric division of Drosophila neuroblast
    T Ohshiro
    CREST, Japan Science and Technology Corporation, Sendai
    Nature 408:593-6. 2000
    ..Thus, Lgl and Dlg act in a common process that differentially mediates cortical protein targeting in mitotic neuroblasts, and that creates intrinsic differences between daughter cells...
  17. ncbi The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
    C Y Peng
    Howard Hughes Medical Institute, University of Oregon, Eugene 97403, USA
    Nature 408:596-600. 2000
    ..We conclude that Dlg and Lgl promote, and myosin II inhibits, actomyosin-dependent basal protein targeting in neuroblasts...
  18. ncbi Patterns of cell division and expression of asymmetric cell fate determinants in postembryonic neuroblast lineages of Drosophila
    J Ceron
    Instituto de Neurociencias, Universidad Miguel Hernandez y CSIC, Alicante, 03550, Spain
    Dev Biol 230:125-38. 2001
    ..vi) Prospero is transiently expressed in one of the two sibling GCs generated by the division of GMCs. The implications of these results on cell fate specification and differentiation of adult brain neurons are discussed...
  19. ncbi Asymmetric cell division: fly neuroblast meets worm zygote
    C Q Doe
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, Oregon 97403, USA
    Curr Opin Cell Biol 13:68-75. 2001
    ..Mammalian epithelia also use this complex to regulate apical/basal polarity. Recent results have allowed us to compare the mechanisms regulating asymmetric cell division in Drosophila neuroblasts and the C. elegans zygote...
  20. pmc A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions
    Y Cai
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore
    EMBO J 20:1704-14. 2001
    ....
  21. ncbi The Partner of Inscuteable/Discs-large complex is required to establish planar polarity during asymmetric cell division in Drosophila
    Y Bellaiche
    Ecole Normale Superieure, UMR 8544, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Cell 106:355-66. 2001
    ..Finally, Baz and the Dlg/Pins complex are required for the asymmetric localization of Numb. Thus, the Dlg/Pins complex responds to Fz signaling to establish planar asymmetry in the pI cell...
  22. ncbi Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
    S Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Curr Opin Cell Biol 13:641-8. 2001
    ..Recent studies on the role of this PAR-aPKC complex in epithelial cell polarization provide new insights into the molecular basis of epithelial junctional formation and cell polarity...
  23. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
    ..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
  24. pmc Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein
    Fengwei Yu
    Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    Mol Cell Biol 22:4230-40. 2002
    ..A complex of apically localized proteins, including Inscuteable (Insc), Partner of Inscuteable (Pins), Bazooka (Baz), DmPar-6, DaPKC, and G alpha i, is required to mediate and ..
  25. ncbi Asymmetric division of Drosophila neural progenitors
    William Chia
    MRC Centre for Developmental Neurobiology, King s College London, 4th Floor, New Hunts House, Guy s Hospital Campus, London, UK
    Curr Opin Genet Dev 12:459-64. 2002
    ..Recent findings also suggest possible mechanisms by which the processes of cell-cycle progression, neuronal lineage development and asymmetric divisions may be integrated...
  26. pmc Inscuteable-independent apicobasally oriented asymmetric divisions in the Drosophila embryonic CNS
    Priyadarshini Rath
    Developmental Neurobiology Laboratory, Institute of Molecular and Cell Biology, Sinagapore, UK
    EMBO Rep 3:660-5. 2002
    ..We conclude that inscuteable is not required for all apicobasally oriented asymmetric divisions and that, in some cellular contexts, bazooka can mediate apicobasal asymmetric divisions without inscuteable...
  27. ncbi Asymmetric segregation of Numb: a mechanism for neural specification from Drosophila to mammals
    Michel Cayouette
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, University College London, Gower Street, London WC1E 6BT, UK
    Nat Neurosci 5:1265-9. 2002
    ..Here, we review these and other findings that suggest an important role for the asymmetric segregation of Numb proteins in vertebrate neural development...
  28. ncbi Control of cell polarity and mitotic spindle positioning in animal cells
    Julie Ahringer
    Wellcome Trust Cancer Research UK Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Curr Opin Cell Biol 15:73-81. 2003
    ..Microtubules and conserved PAR proteins are essential mediators of cell polarity, and mitotic spindle positioning depends on heterotrimeric G protein signalling and the microtubule motor protein dynein...
  29. ncbi Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry
    Roger Albertson
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon 1254, Eugene, OR 97403, USA
    Nat Cell Biol 5:166-70. 2003
    ..We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in Drosophila neuroblasts...
  30. ncbi The Drosophila myosin VI Jaguar is required for basal protein targeting and correct spindle orientation in mitotic neuroblasts
    Claudia Petritsch
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Cell 4:273-81. 2003
    ..Our studies demonstrate that a class VI myosin is necessary for basal protein targeting and spindle orientation in neuroblasts...
  31. pmc Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
    Melissa M Rolls
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, OR 97403, USA
    J Cell Biol 163:1089-98. 2003
    ....
  32. ncbi Dare to be different: asymmetric cell division in Drosophila, C. elegans and vertebrates
    Jörg Betschinger
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 14:R674-85. 2004
    ..Here, we outline the steps and factors that are involved in this process in Drosophila and C. elegans and discuss their potential conservation in vertebrates...
  33. pmc Locomotion defects, together with Pins, regulates heterotrimeric G-protein signaling during Drosophila neuroblast asymmetric divisions
    Fengwei Yu
    Temasek Lifesciences Laboratory and Department of Biological Sciences, National University of Singapore
    Genes Dev 19:1341-53. 2005
    ..Thus, Loco can potentially regulate heterotrimeric G-protein signaling via two distinct modes of action during Drosophila neuroblast asymmetric divisions...
  34. ncbi Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system
    Gregory Emery
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Cell 122:763-73. 2005
    ..Our results suggest that cells can regulate signaling pathways and influence their developmental fate by inhibiting the formation of individual endocytic compartments...
  35. ncbi Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
    Hongyan Wang
    Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
    Nat Cell Biol 7:1091-8. 2005
    ..In addition, Gbetagamma acts to restrict Galphai (and GoLoco proteins) to the apical cortex, where Galphai (and Pins) can mediate asymmetric spindle geometry...
  36. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
    ..These results identify a role for microtubules in polarizing the neuroblast cortex, a fundamental step for generating cell diversity through asymmetric cell division...
  37. ncbi Extrinsic cues orient the cell division axis in Drosophila embryonic neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, 97403, USA
    Development 133:529-36. 2006
    ..This ensures the proper coordination between neuroblast cell polarity and CNS tissue polarity...
  38. ncbi Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells
    Joerg Betschinger
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Cell 124:1241-53. 2006
    ..Similar defects are seen in lethal giant larvae (lgl) mutants where Brat and Prospero are not asymmetric. We have identified a molecular mechanism that may control self-renewal and prevent tumor formation in other stem cells as well...
  39. ncbi Drosophila Pins-binding protein Mud regulates spindle-polarity coupling and centrosome organization
    Yasushi Izumi
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, and CREST, Japan Science and Technology Corporation, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe 650 0047, Japan
    Nat Cell Biol 8:586-93. 2006
    ..We propose that Drosophila Mud, vertebrate NuMA and Caenorhabditis elegans Lin-5 (refs 5, 6) have conserved roles in the mechanism by which G-proteins regulate the mitotic spindle...
  40. pmc Drosophila homologs of mammalian TNF/TNFR-related molecules regulate segregation of Miranda/Prospero in neuroblasts
    Huashan Wang
    Drosophila Neurobiology Lab, Institute of Molecular and Cell Biology, Proteos Building, 61 Biopolis Drive, Singapore 138673, Singapore
    EMBO J 25:5783-93. 2006
    ..The Mira/Pros telophase rescue seen in inscuteable mutant NBs requires DTRAF1. Our data suggest that DTRAF1 binds to Baz and acts downstream of Egr in the Mira/Pros telophase rescue pathway...
  41. pmc Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe
    Boris Egger
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Neural Dev 2:1. 2007
    ..In some tissues cell fate appears to dictate the type of cell division, whereas in other tissues it is thought that stochastic variation in spindle position dictates subsequent sibling cell fate...
  42. pmc Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts
    Rick W Nipper
    Institute of Molecular Biology, Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 104:14306-11. 2007
    ..We conclude that Pins has multiple activity states that generate cortical polarity and link it with mitotic spindle orientation...
  43. pmc The tumor suppressors Brat and Numb regulate transit-amplifying neuroblast lineages in Drosophila
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Dev Cell 14:535-46. 2008
    ..Our data describe a transit-amplifying lineage in the Drosophila nervous system and suggest that different vulnerabilities in intermediate cell types can affect the outcome of tumor suppressor loss in stem cell lineages...
  44. doi The PDZ protein Canoe regulates the asymmetric division of Drosophila neuroblasts and muscle progenitors
    Stephan Speicher
    Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Científicas University Miguel Hernandez, Sant Joan d Alacant, 03550 Alicante, Spain
    Curr Biol 18:831-7. 2008
    ..Here, we show that Cno functions as a new effector of the apical proteins Inscuteable (Insc)-Partner of Inscuteable (Pins)-Galphai during the asymmetric division of Drosophila neuroblasts (NBs)...
  45. pmc Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization
    Frederik Wirtz-Peitz
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 135:161-73. 2008
    ..Our data reveal a molecular mechanism for the asymmetric localization of Numb and show how cell polarity can be coupled to cell-cycle progression...
  46. pmc Protein phosphatase 4 mediates localization of the Miranda complex during Drosophila neuroblast asymmetric divisions
    Rita Sousa-Nunes
    Department of Biological Sciences, Temasek Life Sciences Laboratory, National University of Singapore, Singapore
    Genes Dev 23:359-72. 2009
    ..Our findings suggest that Flfl may target PP4 to the MIra protein complex to facilitate dephosphorylation step(s) crucial for its cortical association/asymmetric localization...
  47. doi On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages
    Gerald Udolph
    Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore
    Dev Biol 336:156-68. 2009
    ..g., Notch (N), Delta (Dl), and kuzbanian (kuz) and a crucial regulator of asymmetric cell division, inscuteable (insc) throughout lineage progression of 4 neuroblasts (NB1-1, MP2, NB4-2, and NB7-1)...
  48. ncbi Asymmetric division and polarity of neuroepithelial cells
    W B Huttner
    Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany
    Curr Opin Neurobiol 7:29-39. 1997
    ....
  49. ncbi Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions
    P Li
    Developmental Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore
    Cell 90:437-47. 1997
    ..Stau, as one component of this complex, is required only for RNA localization. Hence staufen also acts zygotically, downstream of inscuteable, to effect aspects of neuroblast asymmetry...
  50. ncbi Identification of Miranda protein domains regulating asymmetric cortical localization, cargo binding, and cortical release
    S Fuerstenberg
    Department of Cell and Structural Biology, University of Illinois, Urbana, Illinois, 61801, USA
    Mol Cell Neurosci 12:325-39. 1998
    ..Finally, we define a C-terminal domain of Staufen sufficient for Miranda-dependent cortical localization in neuroblasts...
  51. ncbi Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization
    F Yu
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Cell 100:399-409. 2000
    Asymmetric localization is a prerequisite for inscuteable (insc) to function in coordinating and mediating asymmetric cell divisions in Drosophila...
  52. ncbi DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
    M Petronczki
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Nat Cell Biol 3:43-9. 2001
    ....
  53. ncbi Adherens junctions inhibit asymmetric division in the Drosophila epithelium
    B Lu
    Howard Hughes Medical Institute and Department of Physiology, University of California at San Francisco, 94143 0725, USA
    Nature 409:522-5. 2001
    ..Our results indicate that neuroepithelial cells have all the necessary components to execute asymmetric division, but that this pathway is normally overridden by the planar polarity cue provided by adherens junctions...
  54. ncbi cdc2 links the Drosophila cell cycle and asymmetric division machineries
    M Tio
    Institute of Molecular and Cell Biology, Singapore
    Nature 409:1063-7. 2001
    ..Our findings link cdc2 with asymmetric divisions, and explain why the asymmetric localization of molecules like Inscuteable show cell-cycle dependence...
  55. ncbi Apical complex genes control mitotic spindle geometry and relative size of daughter cells in Drosophila neuroblast and pI asymmetric divisions
    Yu Cai
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore
    Cell 112:51-62. 2003
    ..In sensory organ precursors, Bazooka/DaPKC and Pins/G alpha i localize to opposite sides of the cortex and function in opposition to generate a symmetric spindle...
  56. pmc Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila
    C P Shen
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0725 USA
    Genes Dev 12:1837-46. 1998
    ..Our results suggest that Miranda directs the basal cortical localization of multiple molecules, including Staufen and prospero RNA, in mitotic neuroblasts in an actin-dependent manner...
  57. ncbi Cell polarity: the PARty expands
    C Q Doe
    Institute of Molecular Biology and the Institute of Neuroscience, HHMI, University of Oregon, Eugene, Oregon 97403, USA
    Nat Cell Biol 3:E7-9. 2001
    ..This common cell-polarity mechanism is used in cell-specific ways, as highlighted by the recent finding that at least two different types of asymmetric division are observed in Drosophila neural precursors...
  58. ncbi Asymmetric cell division: microtubule dynamics and spindle asymmetry
    Julia A Kaltschmidt
    Wellcome Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    J Cell Sci 115:2257-64. 2002
    ..In this commentary we discuss recent findings on how the mitotic spindle is positioned and on cleavage site induction and place them in the context of cell size asymmetry in different model organisms...
  59. ncbi Drosophila pod-1 crosslinks both actin and microtubules and controls the targeting of axons
    Michael E Rothenberg
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, 533 Parnassus Avenue, San Francisco, CA 94143, USA
    Neuron 39:779-91. 2003
    ..Taken together, these results reveal novel activities for pod-1 and show that proper levels of Dpod1, an actin/MT crosslinker, must be maintained in the growth cone for correct axon guidance...
  60. ncbi Mother-daughter precursor cell fate transformation after Cdc2 down-regulation in the Drosophila bristle lineage
    Pierre Fichelson
    UMR 7622, CNRS Universite Paris VI, 9, quai St Bernard, 75252 Paris Cedex 05, France
    Dev Biol 276:367-77. 2004
    ..These transformations in cell identity suggest that, although synchronized, cell cycle and fate determination are independent phenomena in the bristle lineage...
  61. ncbi Epithelial differentiation in Drosophila
    U Tepass
    Department of Zoology, University of Toronto, Ontario, Canada
    Bioessays 19:673-82. 1997
    ..The combination of embryological and genetic/molecular tools in Drosophila will help us to elucidate the complex events that determine epithelial cell structure and how they relate to morphogenesis and other developmental processes...
  62. ncbi Asymmetric cell division
    Y N Jan
    Howard Hughes Medical Institute, Department of Physiology, University of California at San Francisco, 94143 0725, USA
    Nature 392:775-8. 1998
    ....
  63. pmc Mapping of Drosophila mutations using site-specific male recombination
    B Chen
    Department of Pharmacology, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794 8651, USA
    Genetics 149:157-63. 1998
    ..These experiments demonstrate that P-element-induced site-specific male recombination is an efficient and general method to map Drosophila autosomal mutations...
  64. ncbi Asymmetry and cell fate in the Drosophila embryonic CNS
    S Fuerstenberg
    Department of Cell and Structural Biology, University of Illinois, Urbana 61821, USA
    Int J Dev Biol 42:379-83. 1998
    ....
  65. ncbi Asymmetric cell division: lessons from flies and worms
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143 0725, USA
    Curr Opin Genet Dev 8:392-9. 1998
    ..elegans and Frizzled signaling in Drosophila reveals additional mechanisms for modulating cellular polarity and suggests that there are some similarities between the two systems...
  66. ncbi Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization
    J A Knoblich
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco 94143 0725, USA
    Curr Biol 9:155-8. 1999
    ....
  67. ncbi Genetics of heart development
    J N Chen
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Trends Genet 16:383-8. 2000
    ..Mutational analysis in mice and, as particular focus of this review, in zebrafish, reveals the additional genes brought into play to fashion these evolutionarily 'new' organotypic components...
  68. ncbi Two types of asymmetric divisions in the Drosophila sensory organ precursor cell lineage
    F Roegiers
    Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, California 94143 0725, USA
    Nat Cell Biol 3:58-67. 2001
    ..On the basis of these observations, we propose that two distinct mechanisms for controlling asymmetric cell divisions occur within the same lineage in the developing peripheral nervous system in Drosophila...
  69. ncbi Spindles cotton on to junctions, APC and EB1
    M Bienz
    Nat Cell Biol 3:E67-8. 2001
  70. ncbi PAR-1 is required for the maintenance of oocyte fate in Drosophila
    J R Huynh
    The Wellcome CRC Institute, University of Cambridge, Tennis Court Rd, Cambridge CB2 1QR, UK
    Development 128:1201-9. 2001
    ..Finally, we show that PAR-1 localises on the fusome, and provides a link between the asymmetry of the fusome and the selection of the oocyte...
  71. ncbi Asymmetric cell division: plane but not simple
    P N Adler
    University of Virginia, Department of Biology and Cancer Center, Charlottesville, Virginia 22903, USA
    Curr Biol 11:R233-6. 2001
    ..The orientation of these divisions is under the control of the Frizzled pathway that generates planar polarity in a number of cell types...
  72. ncbi The COE--Collier/Olf1/EBF--transcription factors: structural conservation and diversity of developmental functions
    L Dubois
    Centre de Biologie du Developpement, UMR 5547, CNRS Université Paul Sabatier, 118 route de Narbonne 31062 Toulouse cedex 04, France
    Mech Dev 108:3-12. 2001
    ..We also discuss the existence of an evolutionarily conserved pathway linking Notch signalling and COE regulatory functions in various developmental decisions...
  73. ncbi Asymmetric Prospero localization is required to generate mixed neuronal/glial lineages in the Drosophila CNS
    M R Freeman
    HHMI, Institute of Neuroscience, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Development 128:4103-12. 2001
    ....
  74. ncbi Slit signaling promotes the terminal asymmetric division of neural precursor cells in the Drosophila CNS
    B Mehta
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Development 128:3161-8. 2001
    ..Consistent with this, over-expression of these POU genes in a late GMC-1 causes mis-localization of Insc and symmetric division of GMC-1 to generate two RP2s...
  75. ncbi The Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila
    S I Ashraf
    Program in Molecular Medicine and Department of Cell Biology, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Development 128:4757-67. 2001
    ..These results suggest that the Snail family of proteins control both asymmetry and cell division of neuroblasts by activating, probably indirectly, the expression of inscuteable and string...
  76. ncbi Heterotrimeric G proteins: new tricks for an old dog
    Bernhard Hampoelz
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Cell 119:453-6. 2004
    ..2004), and Current Biology (Couwenbergs et al., 2004) show that this function is conserved in vertebrates and--like the classical pathway--involves cycling of G proteins between GDP and GTP bound conformations...
  77. ncbi Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling
    Walter von Stein
    Abteilung Stammzellbiologie, CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    Development 132:1675-86. 2005
    ..Pten mutant embryos also show severe defects during cellularization. Our data provide evidence for a link between the PAR/aPKC complex, the actin cytoskeleton and PI3-kinase signaling mediated by PTEN...
  78. ncbi Drosophila Ric-8 is essential for plasma-membrane localization of heterotrimeric G proteins
    Bernhard Hampoelz
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Nat Cell Biol 7:1099-105. 2005
    ..Our results indicate a model in which both receptor-dependent and receptor-independent G-protein functions are executed at the plasma membrane and require the Ric-8 protein...
  79. ncbi Playing ping pong with pins: cortical and microtubule-induced polarity
    Julie Ahringer
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1184-6. 2005
    ..In this issue of Cell, it is turned around and shown that the transfer of polarity information between the cortex and the spindle is not just one way. In Drosophila neuroblasts, the spindle also has polarizing activity on the cortex...
  80. pmc A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions
    Cathy Slack
    Temasek Lifesciences Laboratory, The National University of Singapore, 117604, Singapore
    BMC Genet 7:33. 2006
    ....
  81. ncbi Asymmetric localisation of Miranda and its cargo proteins during neuroblast division requires the anaphase-promoting complex/cyclosome
    Cathy Slack
    MRC Centre for Developmental Neurobiology, New Hunt s House, King s College London, Guy s Campus, London SE1 1UL, UK
    Development 134:3781-7. 2007
    ..Our results demonstrate that APC/C activity and ubiquitylation of Miranda are required for the asymmetric localisation of Miranda and its cargo proteins to the NB cortex...
  82. doi The Drosophila wing hearts originate from pericardial cells and are essential for wing maturation
    Markus Tögel
    Department of Biology, University of Osnabruck, Zoology Developmental Biology, Barbarastrasse 11, D 49069 Osnabruck, Germany
    Dev Biol 318:29-37. 2008
    ..This indicates that wing hearts are required for proper wing morphogenesis and functionality...
  83. ncbi Asymmetric cell division and neurogenesis
    C Q Doe
    Department of Cell and Structural Biology, University of Illinois, Urbana, Illinois 61801, USA
    Curr Opin Genet Dev 6:562-6. 1996
    ..New insights into how cell diversity is generated during neurogenesis have been obtained and the means for rapid progress in this field now exist...
  84. ncbi Neuroblasts: a model for the asymmetric division of stem cells
    H Lin
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Trends Genet 13:33-9. 1997
    ..These findings are illuminating in understanding the general mechanism of stem cell division...
  85. ncbi Pre-empting the arrival of a dark lord
    Y N Jan
    Nature 389:665. 1997
  86. ncbi Somatic mesoderm differentiation and the development of a subset of pericardial cells depend on the not enough muscles (nem) locus, which contains the inscuteable gene and the intron located gene, skittles
    S Knirr
    Fachbereich Biologie, Zoologie Entwicklungsbiologie, Philipps University Marburg, Germany
    Mech Dev 67:69-81. 1997
    b>Not enough muscles (nem) mutants of Drosophila reveal defects in the development of embryonic muscles, a subset of pericardial cells, the CNS and derivatives of the PNS (Burchard, S., Paululat, A., Hinz, U. and Renkawitz-Pohl, R...
  87. ncbi Asymmetic division: dynastic intricacies of neuroblast division
    J A Campos-Ortega
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Germany
    Curr Biol 7:R726-8. 1997
    ..The proteins encoded by the genes inscuteable, staufen and miranda are involved in the localisation of Prospero...
  88. ncbi Extrinsic cues, intrinsic cues and microfilaments regulate asymmetric protein localization in Drosophila neuroblasts
    J Broadus
    Department of Cell and Structural Biology, Howard Hughes Medical Institute, University of Illinois, Urbana 61801, USA
    Curr Biol 7:827-35. 1997
    ..Here we use in vitro culture of neuroblasts to investigate the role of intrinsic and extrinsic cues and the cytoskeleton in asymmetric localization of Inscuteable, Prospero and Staufen proteins...
  89. ncbi Asymmetric segregation of the Drosophila numb protein during mitosis: facts and speculations
    J A Knoblich
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco 94143 0724, USA
    Cold Spring Harb Symp Quant Biol 62:71-7. 1997
  90. ncbi On the roles of inscuteable in asymmetric cell divisions in Drosophila
    W Chia
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Cold Spring Harb Symp Quant Biol 62:79-87. 1997
    Three processes requiring insc, protein localization, RNA localization, and mitotic spindle orientation, appear to be tightly correlated but nevertheless independent...
  91. ncbi Neural stem cells: from fly to vertebrates
    C Q Doe
    Department of Cell and Structural Biology, HHMI, University of Illinois, Urbana 61821, USA
    J Neurobiol 36:111-27. 1998
    ..It has become increasingly clear that vertebrates and Drosophila share many fundamental mechanisms of neurogenesis, validating a comparative approach...
  92. ncbi Requirement for the Drosophila COE transcription factor Collier in formation of an embryonic muscle: transcriptional response to notch signalling
    M Crozatier
    Centre de Biologie du Developpement, UMR 5547 CNRS UPS, France
    Development 126:1495-504. 1999
    ....
  93. ncbi Control of muscle fibre and motoneuron diversification
    S M Hughes
    MRC Muscle and Cell Motility Unit Developmental Biology Research Centre The Randall Institute King s College London 26 29 Drury Lane London WC2B 5RL UK
    Curr Opin Neurobiol 9:54-64. 1999
    ..We propose a model in which four classes of decision control the patterning of both motoneurons and muscles...
  94. ncbi Jumeaux, a novel Drosophila winged-helix family protein, is required for generating asymmetric sibling neuronal cell fates
    P Y Cheah
    Institute of Molecular and Cell Biology, National University of Singapore Campus, Singapore 117609
    Development 127:3325-35. 2000
    ..During the division of the first born GMC from the NB4-2 lineage, GMC4-2a, Inscuteable (Insc) is localised to the apical cortex, Pon/Numb is localised to the basal cortex and two daughters with distinct ..
  95. ncbi Mechanism of glia-neuron cell-fate switch in the Drosophila thoracic neuroblast 6-4 lineage
    Y Akiyama-Oda
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Development 127:3513-22. 2000
    ..Furthermore, we showed that outside the NB6-4T lineage most glial cells appeared independently of Pros...
  96. ncbi Cell polarity: Scaffold proteins par excellence
    D P Brazil
    Friedrich Miescher Institute, Basel, Switzerland
    Curr Biol 10:R592-4. 2000
    ..Recent studies have identified one of these proteins, Par6, as a key regulator of cell polarity and transformation via its interactions with small GTPases and atypical forms of protein kinase C...
  97. ncbi Tumour-suppressor genes prosper during asymmetric division
    S Greaves
    Nat Cell Biol 3:E11. 2001
  98. ncbi Lineage, cell polarity and inscuteable function in the peripheral nervous system of the Drosophila embryo
    V Orgogozo
    Ecole Normale Superieure, UMR 8544 46, rue d Ulm, 75230 Paris Cedex 05, France
    Development 128:631-43. 2001
    ..This study establishes for the first time the function of Inscuteable in the PNS, and provides the basis for studying the mechanisms controlling planar and apical-basal cell polarities in the embryonic sensory organ lineages...
  99. ncbi Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila
    J R Huynh
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, CB21QR, Cambridge, United Kingdom
    Curr Biol 11:901-6. 2001
    ..elegans and Drosophila, the relationships between them are different, as the localization of PAR-1 does not require Bazooka or PAR-6 in Drosophila, as it does in the worm...
  100. ncbi G protein signaling and asymmetric cell division
    E Knust
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Cell 107:125-8. 2001
    ..Receptor-independent activation of heterotrimeric G proteins by the Drosophila GoLoco protein Partner of Inscuteable seems to represent a novel mechanism to control these events...
  101. ncbi Inscuteable-dependent apical localization of the microtubule-binding protein Cornetto suggests a role in asymmetric cell division
    S Bulgheresi
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    J Cell Sci 114:3655-62. 2001
    ....

Research Grants11

  1. REGULATION OF NEUROGENESIS IN THE DROSOPHILA CNS
    Krishna Bhat; Fiscal Year: 2002
    ..These studies will help understand pathways that govern the lineage elaboration in the Drosophila CNS as well as in other eukaryotic organisms including humans. ..
  2. GENETIC ANALYSIS OF PREHAIR MORPHOGENESIS
    Paul Adler; Fiscal Year: 2005
    ....
  3. DROSOPHILA SENSORY NEURON DEVELOPMENT
    Volker Hartenstein; Fiscal Year: 2006
    ..We expect that the results of these proposed aims will apply to neural development widely (in particular to vertebrates) because the high degree of conservation between morphogenetic processes shaping animal brains. ..
  4. Niemann Pick C disease in Drosophila
    Krishna Bhat; Fiscal Year: 2007
    ..This work will provide insight into the role of these proteins in neurodegenerative diseases and development. ..
  5. DROSOPHILA SENSORY NEURON DEVELOPMENT
    Volker Hartenstein; Fiscal Year: 2007
    ..abstract_text> ..
  6. Patterning of the Primitive Heart Tube in Zebrafish
    Jau Nian Chen; Fiscal Year: 2010
    ..The combination of cellular, molecular and genetic studies proposed in this project will provide new and in-depth insight into mechanisms of primitive heart tube morphogenesis. ..
  7. Genetic Control of Intestinal Stem Cells in the Drosophila Hindgut
    Volker Hartenstein; Fiscal Year: 2010
    ..abstract_text> ..
  8. REGIONAL PATTERNING IN THE VENTRAL TELENCEPHALON
    GORDON J FISHELL; Fiscal Year: 2010
    ..If we can understand how this population is generated, we will take the first step towards assessing whether we can use gene or cell therapies to ameliorate the devasting consequences associated with cortical interneuron dysfunction. ..
  9. MOLECULAR GENETIC ANALYSIS OF ASYMMETRIC CELL DIVISIONS
    Chris Doe; Fiscal Year: 2002
    ..We will do RNA and protein localization studies; our collaborator, Dr. G. Oliver will generate and assay the gene knock-out mice. ..
  10. Organogenesis Screen Using Tissue-Specific Zebrafish
    Jau Nian Chen; Fiscal Year: 2005
    ..Mutations identified from this screen will provide entry points to dissect genetic and molecular pathways involved in vertebrate organogenesis. ..
  11. MECHANISMS OF NEURAL PATTERNING IN MAMMALIAN FOREBRAIN
    Gordon Fishell; Fiscal Year: 2007
    ..abstract_text> ..