mira

Summary

Gene Symbol: mira
Description: miranda
Alias: CG12249, Dmel\CG12249, MIR, MIRA, Mir, Mira, Miranda, mir, CG12249 gene product from transcript CG12249-RB, CG12249-PA, CG12249-PB, CG12249-PC, mira-PA, mira-PB, mira-PC
Species: fruit fly

Top Publications

  1. pmc Miranda mediates asymmetric protein and RNA localization in the developing nervous system
    A J Schuldt
    Wellcome CRC Institute and Department of Genetics, Cambridge CB2 1QR, UK
    Genes Dev 12:1847-57. 1998
  2. pmc A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions
    Y Cai
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore
    EMBO J 20:1704-14. 2001
  3. ncbi The brain tumor gene negatively regulates neural progenitor cell proliferation in the larval central brain of Drosophila
    Bruno Bello
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Development 133:2639-48. 2006
  4. ncbi Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells
    Joerg Betschinger
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Cell 124:1241-53. 2006
  5. ncbi Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila
    J R Huynh
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, CB21QR, Cambridge, United Kingdom
    Curr Biol 11:901-6. 2001
  6. pmc The tumor suppressors Brat and Numb regulate transit-amplifying neuroblast lineages in Drosophila
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Dev Cell 14:535-46. 2008
  7. ncbi Brat is a Miranda cargo protein that promotes neuronal differentiation and inhibits neuroblast self-renewal
    Cheng Yu Lee
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, 97403, USA
    Dev Cell 10:441-9. 2006
  8. ncbi The Drosophila NuMA Homolog Mud regulates spindle orientation in asymmetric cell division
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Dev Cell 10:731-42. 2006
  9. ncbi A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila
    M Schaefer
    Research Institute of Molecular Pathology IMP, Vienna, A 1030, Austria
    Curr Biol 10:353-62. 2000
  10. pmc Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe
    Boris Egger
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Neural Dev 2:1. 2007

Research Grants

Scientific Experts

Detail Information

Publications127 found, 100 shown here

  1. pmc Miranda mediates asymmetric protein and RNA localization in the developing nervous system
    A J Schuldt
    Wellcome CRC Institute and Department of Genetics, Cambridge CB2 1QR, UK
    Genes Dev 12:1847-57. 1998
    ..During these divisions, the cell fate determinant Prospero is asymmetrically partitioned to the GMC by Miranda protein, which tethers it to the basal cortex of the dividing neuroblast...
  2. pmc A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions
    Y Cai
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore
    EMBO J 20:1704-14. 2001
    ....
  3. ncbi The brain tumor gene negatively regulates neural progenitor cell proliferation in the larval central brain of Drosophila
    Bruno Bello
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Development 133:2639-48. 2006
    ....
  4. ncbi Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells
    Joerg Betschinger
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Cell 124:1241-53. 2006
    ..Like Prospero, Brat binds and cosegregates with the adaptor protein Miranda. In larval neuroblasts, both Brat and Prospero are required to inhibit self-renewal in one of the two daughter ..
  5. ncbi Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila
    J R Huynh
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, CB21QR, Cambridge, United Kingdom
    Curr Biol 11:901-6. 2001
    ..elegans and Drosophila, the relationships between them are different, as the localization of PAR-1 does not require Bazooka or PAR-6 in Drosophila, as it does in the worm...
  6. pmc The tumor suppressors Brat and Numb regulate transit-amplifying neuroblast lineages in Drosophila
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Dev Cell 14:535-46. 2008
    ..Our data describe a transit-amplifying lineage in the Drosophila nervous system and suggest that different vulnerabilities in intermediate cell types can affect the outcome of tumor suppressor loss in stem cell lineages...
  7. ncbi Brat is a Miranda cargo protein that promotes neuronal differentiation and inhibits neuroblast self-renewal
    Cheng Yu Lee
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, 97403, USA
    Dev Cell 10:441-9. 2006
    ..report that the Brain tumor (Brat) translation repressor is partitioned into GMCs via direct interaction with the Miranda scaffolding protein...
  8. ncbi The Drosophila NuMA Homolog Mud regulates spindle orientation in asymmetric cell division
    Sarah K Bowman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Dev Cell 10:731-42. 2006
    ..Our data suggest a model in which asymmetrically localized Pins-G alphai complexes regulate spindle orientation by directly binding to Mud...
  9. ncbi A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila
    M Schaefer
    Research Institute of Molecular Pathology IMP, Vienna, A 1030, Austria
    Curr Biol 10:353-62. 2000
    ..How apically localised Inscuteable directs asymmetric cell divisions is unclear...
  10. pmc Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe
    Boris Egger
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Neural Dev 2:1. 2007
    ..In some tissues cell fate appears to dictate the type of cell division, whereas in other tissues it is thought that stochastic variation in spindle position dictates subsequent sibling cell fate...
  11. ncbi Asymmetric cell division
    Y N Jan
    Howard Hughes Medical Institute, Department of Physiology, University of California at San Francisco, 94143 0725, USA
    Nature 392:775-8. 1998
    ....
  12. ncbi miranda localizes staufen and prospero asymmetrically in mitotic neuroblasts and epithelial cells in early Drosophila embryogenesis
    F Matsuzaki
    Department of Molecular Genetics, National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187, Japan
    Development 125:4089-98. 1998
    ..b>miranda is known to localize prospero protein to the basal cell cortex of neuroblasts while the staufen RNA-binding ..
  13. ncbi Bazooka recruits Inscuteable to orient asymmetric cell divisions in Drosophila neuroblasts
    M Schober
    Research Institute of Molecular Pathology, Vienna, Austria
    Nature 402:548-51. 1999
    ..Several proteins, including Numb and Miranda, segregate into the basal daughter cell and are needed for the determination of its correct cell fate...
  14. doi Temporal transcription factors and their targets schedule the end of neural proliferation in Drosophila
    Cédric Maurange
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Cell 133:891-902. 2008
    ..These studies identify the timing mechanism ending CNS proliferation and reveal how aging progenitors transduce bursts of transcription factors into long-lasting changes in cell proliferation and cell identity...
  15. ncbi Miranda directs Prospero to a daughter cell during Drosophila asymmetric divisions
    H Ikeshima-Kataoka
    Department of Molecular Genetics, National Institute of Neuroscience, Tokyo, Japan
    Nature 390:625-9. 1997
    ..Here we report the identification of a gene, miranda, which encodes a new protein that co-localizes with Prospero in mitotic neuroblasts, tethers Prospero to the ..
  16. ncbi Identification of Miranda protein domains regulating asymmetric cortical localization, cargo binding, and cortical release
    S Fuerstenberg
    Department of Cell and Structural Biology, University of Illinois, Urbana, Illinois, 61801, USA
    Mol Cell Neurosci 12:325-39. 1998
    ..Establishing neuroblast/GMC differences involves the asymmetric localization of proteins (Inscuteable, Miranda, Prospero, and Staufen) and RNA (prospero)...
  17. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
    ..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
  18. ncbi Asymmetric division of Drosophila neural progenitors
    William Chia
    MRC Centre for Developmental Neurobiology, King s College London, 4th Floor, New Hunts House, Guy s Hospital Campus, London, UK
    Curr Opin Genet Dev 12:459-64. 2002
    ..Recent findings also suggest possible mechanisms by which the processes of cell-cycle progression, neuronal lineage development and asymmetric divisions may be integrated...
  19. ncbi Heterotrimeric G proteins regulate daughter cell size asymmetry in Drosophila neuroblast divisions
    Naoyuki Fuse
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, Chuou ku, Kobe 650 0047, Japan
    Curr Biol 13:947-54. 2003
    ..Furthermore, the multiple equal cleavages of G beta mutant neuroblasts accompany neural defects; this finding suggests indispensable roles of eccentric division in assuring the stem cell properties of neuroblasts...
  20. ncbi Dare to be different: asymmetric cell division in Drosophila, C. elegans and vertebrates
    Jörg Betschinger
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 14:R674-85. 2004
    ..Here, we outline the steps and factors that are involved in this process in Drosophila and C. elegans and discuss their potential conservation in vertebrates...
  21. pmc Locomotion defects, together with Pins, regulates heterotrimeric G-protein signaling during Drosophila neuroblast asymmetric divisions
    Fengwei Yu
    Temasek Lifesciences Laboratory and Department of Biological Sciences, National University of Singapore
    Genes Dev 19:1341-53. 2005
    ..Thus, Loco can potentially regulate heterotrimeric G-protein signaling via two distinct modes of action during Drosophila neuroblast asymmetric divisions...
  22. ncbi Extrinsic cues orient the cell division axis in Drosophila embryonic neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, 97403, USA
    Development 133:529-36. 2006
    ..This ensures the proper coordination between neuroblast cell polarity and CNS tissue polarity...
  23. ncbi Drosophila Pins-binding protein Mud regulates spindle-polarity coupling and centrosome organization
    Yasushi Izumi
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, and CREST, Japan Science and Technology Corporation, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe 650 0047, Japan
    Nat Cell Biol 8:586-93. 2006
    ..We propose that Drosophila Mud, vertebrate NuMA and Caenorhabditis elegans Lin-5 (refs 5, 6) have conserved roles in the mechanism by which G-proteins regulate the mitotic spindle...
  24. pmc Polo inhibits progenitor self-renewal and regulates Numb asymmetry by phosphorylating Pon
    Hongyan Wang
    Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore 117604
    Nature 449:96-100. 2007
    ..Our results reveal a biochemical link between the cell cycle and the asymmetric protein localization machinery, and indicate that Polo can inhibit progenitor self-renewal by regulating the localization and function of Numb...
  25. ncbi Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila
    C P Shen
    Howard Hughes Medical Institute and Department of Physiology, University of California at San Francisco, 94143 0724, USA
    Cell 90:449-58. 1997
    ..We have isolated a novel gene, miranda, based on the ability of its gene product to interact with the Prospero asymmetric localization domain...
  26. ncbi Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143 0725, USA
    Cell 95:225-35. 1998
    ..We propose that PON is one component of a multimolecular machinery that localizes Numb by responding to polarity cues conserved in neural precursors and epithelial cells...
  27. ncbi Asymmetric cell division: from A to Z
    N Hawkins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genes Dev 12:3625-38. 1998
  28. ncbi Asymmetric division of Drosophila neural stem cells: a basis for neural diversity
    F Matsuzaki
    CREST, Japan Science and Technology Corporation, Department of Developmental Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Aoba ku, 980 8575, Japan tohoku ac jp
    Curr Opin Neurobiol 10:38-44. 2000
    ..Our understanding now extends to the molecular nature of the cell polarity that underlies asymmetric divisions. This polarity is conserved among neural stem cells, epithelial cells and fertilized eggs...
  29. ncbi Rapsynoid/partner of inscuteable controls asymmetric division of larval neuroblasts in Drosophila
    M L Parmentier
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    J Neurosci 20:RC84. 2000
    ..Our data show that Raps is a novel protein involved in the control of asymmetric divisions of neuroblasts...
  30. ncbi Role of cortical tumour-suppressor proteins in asymmetric division of Drosophila neuroblast
    T Ohshiro
    CREST, Japan Science and Technology Corporation, Sendai
    Nature 408:593-6. 2000
    ..Thus, Lgl and Dlg act in a common process that differentially mediates cortical protein targeting in mitotic neuroblasts, and that creates intrinsic differences between daughter cells...
  31. ncbi The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
    C Y Peng
    Howard Hughes Medical Institute, University of Oregon, Eugene 97403, USA
    Nature 408:596-600. 2000
    ..We conclude that Dlg and Lgl promote, and myosin II inhibits, actomyosin-dependent basal protein targeting in neuroblasts...
  32. ncbi Patterns of cell division and expression of asymmetric cell fate determinants in postembryonic neuroblast lineages of Drosophila
    J Ceron
    Instituto de Neurociencias, Universidad Miguel Hernandez y CSIC, Alicante, 03550, Spain
    Dev Biol 230:125-38. 2001
    ..v) Prospero does not colocalize with Miranda in the cortical crescent of mitotic Nbs...
  33. ncbi Asymmetric cell division: fly neuroblast meets worm zygote
    C Q Doe
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, Oregon 97403, USA
    Curr Opin Cell Biol 13:68-75. 2001
    ..Mammalian epithelia also use this complex to regulate apical/basal polarity. Recent results have allowed us to compare the mechanisms regulating asymmetric cell division in Drosophila neuroblasts and the C. elegans zygote...
  34. ncbi The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts
    M G Giansanti
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, Italy
    Development 128:1137-45. 2001
    ..During neuroblast division, several cell fate determinants, such as Miranda, Prospero and Numb, are preferentially segregated into the GMC, ensuring its correct developmental fate...
  35. ncbi Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts
    Y Hong
    Howard Hughes Medical Institute, Department of Physiology and Biochemistry, University of California San Francisco, San Francisco, California 94143 0725, USA
    Nature 414:634-8. 2001
    ..However, for the subset of ectodermal cells that delaminate and form neuroblasts, their polarity requires the function of Bazooka, but not of Stardust or Crumbs...
  36. ncbi Miranda, a protein involved in neuroblast asymmetric division, is associated with embryonic centrosomes of Drosophila melanogaster
    Cristiana Mollinari
    Laboratoire des protéines du Cytosquelette, Institut de Biologie Structurale, Grenoble, France
    Biol Cell 94:1-13. 2002
    b>Miranda, a highly coiled-coil protein, has been shown to be one of the key components necessary for the differential segregation of fate-determining factors during neurogenesis in Drosophila embryos...
  37. ncbi Drosophila APC2 and APC1 have overlapping roles in the larval brain despite their distinct intracellular localizations
    Kathryn Akong
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Dev Biol 250:71-90. 2002
    ..Our experiments suggest that this does not result from misregulation of Wg signaling, and thus may involve the cytoskeletal or adhesive roles of APC proteins...
  38. ncbi Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry
    Roger Albertson
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon 1254, Eugene, OR 97403, USA
    Nat Cell Biol 5:166-70. 2003
    ..We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in Drosophila neuroblasts...
  39. ncbi The Drosophila myosin VI Jaguar is required for basal protein targeting and correct spindle orientation in mitotic neuroblasts
    Claudia Petritsch
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Cell 4:273-81. 2003
    ..spindle orientation and basal localization of the cell fate determinants and their adapter proteins such as Miranda. Here we report that Miranda localization requires the unconventional myosin VI Jaguar (Jar)...
  40. ncbi The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
    Jörg Betschinger
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Nature 422:326-30. 2003
    ..apically and directs localization of the cell fate determinants Prospero and Numb and the adaptor proteins Miranda and Pon to the basal cell cortex, to ensure their segregation into the basal daughter cell...
  41. pmc Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
    Melissa M Rolls
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, OR 97403, USA
    J Cell Biol 163:1089-98. 2003
    ..Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large ..
  42. ncbi Drosophila nonmuscle myosin II promotes the asymmetric segregation of cell fate determinants by cortical exclusion rather than active transport
    Claudia S Barros
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Dev Cell 5:829-40. 2003
    ..Therefore, the movement of myosin II to the contractile ring not only initiates cytokinesis but also completes the partitioning of cell fate determinants from the neuroblast to its daughter...
  43. pmc Differential functions of G protein and Baz-aPKC signaling pathways in Drosophila neuroblast asymmetric division
    Yasushi Izumi
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe 650 0047, Japan
    J Cell Biol 164:729-38. 2004
    ..apical components, Bazooka-atypical PKC-Par6-Inscuteable, still remain polarized and responsible for asymmetric Miranda localization, suggesting their dominant role in localizing cell-fate determinants...
  44. ncbi Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling
    Walter von Stein
    Abteilung Stammzellbiologie, CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    Development 132:1675-86. 2005
    ..Pten mutant embryos also show severe defects during cellularization. Our data provide evidence for a link between the PAR/aPKC complex, the actin cytoskeleton and PI3-kinase signaling mediated by PTEN...
  45. ncbi Drosophila Grainyhead specifies late programmes of neural proliferation by regulating the mitotic activity and Hox-dependent apoptosis of neuroblasts
    Caterina Cenci
    Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 132:3835-45. 2005
    ..This study shows how a factor specific to late-stage neural progenitors can regulate the time at which neural proliferation stops, and identifies mechanisms linking it to the Hox axial patterning system...
  46. ncbi Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster
    Emmanuel Caussinus
    Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Genet 37:1125-9. 2005
    ..We found that larval brain tissue carrying neuroblasts with mutations in raps (also called pins), mira, numb or pros grew to more than 100 times their initial size, invading other tissues and killing the hosts in 2 ..
  47. ncbi Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
    Hongyan Wang
    Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
    Nat Cell Biol 7:1091-8. 2005
    ..In addition, Gbetagamma acts to restrict Galphai (and GoLoco proteins) to the apical cortex, where Galphai (and Pins) can mediate asymmetric spindle geometry...
  48. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
    ..These results identify a role for microtubules in polarizing the neuroblast cortex, a fundamental step for generating cell diversity through asymmetric cell division...
  49. ncbi Connecting cancer to the asymmetric division of stem cells
    Andreas Wodarz
    Department of Stem Cell Biology, DFG Research Center for Molecular Physiology of the Brain CMPB, University of Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    Cell 124:1121-3. 2006
    ..This work suggests a mechanism by which loss of polarity in stem cells may lead to tumorigenesis...
  50. ncbi A primary cell culture of Drosophila postembryonic larval neuroblasts to study cell cycle and asymmetric division
    Julian Ceron
    Instituto de Neurociencias, CSIC and Universidad Miguel Hernandez, San Juan, E 03550 Alicante, Spain
    Eur J Cell Biol 85:567-75. 2006
    ..experiments allowed us to conclude that localization of asymmetric cell division markers such as Inscuteable, Miranda, Prospero and Numb is cell autonomous...
  51. pmc Novel roles for APC family members and Wingless/Wnt signaling during Drosophila brain development
    Melissa A Hayden
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Biol 305:358-76. 2007
    ..Finally, we propose a model suggesting that Wg signaling regulates fine scale cell fates along the anterior-posterior axis, in part by creating an adhesion gradient and consider possible alternate explanations for our observations...
  52. pmc Cdc42 acts downstream of Bazooka to regulate neuroblast polarity through Par-6 aPKC
    Scott X Atwood
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
    J Cell Sci 120:3200-6. 2007
    ..We conclude that Cdc42 regulates aPKC localization and activity downstream of Bazooka, thereby directing neuroblast cell polarity and asymmetric cell division...
  53. pmc Amplification of neural stem cell proliferation by intermediate progenitor cells in Drosophila brain development
    Bruno C Bello
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Neural Dev 3:5. 2008
    ..Here we investigate whether specific neural stem cell-like neuroblasts in the brain of Drosophila might also amplify neuronal proliferation by generating symmetrically dividing intermediate progenitors...
  54. doi Drosophila SPD-2 is an essential centriole component required for PCM recruitment and astral-microtubule nucleation
    Maria Grazia Giansanti
    Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le A Moro 5, 00185 Roma, Italy
    Curr Biol 18:303-9. 2008
    ..These mutations also disrupt proper Miranda localization in dividing NBs, as previously observed in mutants lacking astral MTs [8-10]...
  55. pmc Identification of Drosophila type II neuroblast lineages containing transit amplifying ganglion mother cells
    Jason Q Boone
    Institute of Neuroscience and Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Dev Neurobiol 68:1185-95. 2008
    ..Type II lineages allow more neurons to be produced faster than Type I lineages, which may be advantageous in a rapidly developing organism like Drosophila...
  56. pmc Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression
    Chiswili Chabu
    Institute of Neuroscience, Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
    Development 135:2739-46. 2008
    ..We conclude that Dap160 positively regulates aPKC activity and localization to promote neuroblast cell polarity and cell cycle progression...
  57. doi Centrosome dysfunction in Drosophila neural stem cells causes tumors that are not due to genome instability
    Elisabeth Castellanos
    Cell Division Group, IRB Barcelona, PCB, c Baldiri Reixac 10 12, 08028 Barcelona, Spain
    Curr Biol 18:1209-14. 2008
    ..We propose that such tumors might be caused by impaired asymmetric division of neural stem cells [4]. These results show that centrosome loss, far from being innocuous, is a potentially dangerous condition in flies...
  58. pmc Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization
    Frederik Wirtz-Peitz
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 135:161-73. 2008
    ..Our data reveal a molecular mechanism for the asymmetric localization of Numb and show how cell polarity can be coupled to cell-cycle progression...
  59. doi Neuroblast entry into quiescence is regulated intrinsically by the combined action of spatial Hox proteins and temporal identity factors
    Takuya Tsuji
    Center for Frontier Research, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Development 135:3859-69. 2008
    ....
  60. doi A conserved nuclear receptor, Tailless, is required for efficient proliferation and prolonged maintenance of mushroom body progenitors in the Drosophila brain
    Mitsuhiko Kurusu
    Structural Biology Center, National Institute of Genetics, and Department of Genetics, The Graduate University for Advanced Studies, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Dev Biol 326:224-36. 2009
    ..These results as a whole uncover a distinct regulatory mechanism of self-renewal and differentiation of the MB progenitors that is different from the mechanisms found in other progenitors...
  61. pmc Protein phosphatase 4 mediates localization of the Miranda complex during Drosophila neuroblast asymmetric divisions
    Rita Sousa-Nunes
    Department of Biological Sciences, Temasek Life Sciences Laboratory, National University of Singapore, Singapore
    Genes Dev 23:359-72. 2009
    ..Phosphatase 4 (PP4) regulatory subunit PP4R3/Falafel (Flfl) as a key mediator specific for the localization of Miranda (Mira) and associated cell fate determinants during both interphase and mitosis...
  62. pmc Twins/PP2A regulates aPKC to control neuroblast cell polarity and self-renewal
    Chiswili Chabu
    Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
    Dev Biol 330:399-405. 2009
    ..Cortical aPKC excludes cortical localization of Miranda and its cargo proteins Prospero and Brain tumor, resulting in their partitioning into the differentiating, ..
  63. pmc aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division
    Scott X Atwood
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
    Curr Biol 19:723-9. 2009
    ..apically restricted aPKC is required for segregation of neuronal differentiation factors such as Numb and Miranda to the basal cortical domain...
  64. doi PP2A antagonizes phosphorylation of Bazooka by PAR-1 to control apical-basal polarity in dividing embryonic neuroblasts
    Michael P Krahn
    Abteilung Stammzellbiologie, DFG Research Center for Molecular Physiology of the Brain, Georg August Universitat Gottingen, Gottingen, Germany
    Dev Cell 16:901-8. 2009
    ..Overexpression of PAR-1 or Baz, or mutation of 14-3-3 proteins that bind phosphorylated Baz, causes essentially the same phenotype, indicating that the balance of PAR-1 and PP2A effects on Baz phosphorylation determines NB polarity...
  65. doi Apical/basal spindle orientation is required for neuroblast homeostasis and neuronal differentiation in Drosophila
    Clemens Cabernard
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon 1254, Eugene OR 97403, USA
    Dev Cell 17:134-41. 2009
    ..We conclude that the ratio of apical/basal determinants specifies neuroblast/GMC identity, and that apical/basal spindle orientation is required for neuroblast homeostasis and neuronal differentiation...
  66. pmc Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells
    Christopher A Johnston
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell 138:1150-63. 2009
    ..This "induced cortical polarity" assay is suitable for rapid identification of the proteins, domains, and amino acids regulating spindle orientation or cell polarity...
  67. doi Kinase-activity-independent functions of atypical protein kinase C in Drosophila
    Soya Kim
    Department of Stem Cell Biology, DFG Research Center for Molecular Physiology of the Brain, Georg August University Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    J Cell Sci 122:3759-71. 2009
    ....
  68. doi Cell cycle independent role of Cyclin E during neural cell fate specification in Drosophila is mediated by its regulation of Prospero function
    Christian Berger
    Institute for Genetics, University of Mainz, D 55099 Mainz, Germany
    Dev Biol 337:415-24. 2010
    ..e. asymmetric cell division of NB6-4t. Furthermore our data imply that CycE is required for the maintenance of stem cell identity of most other neuroblasts...
  69. doi dFezf/Earmuff maintains the restricted developmental potential of intermediate neural progenitors in Drosophila
    Mo Weng
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA
    Dev Cell 18:126-35. 2010
    ..We conclude that Erm dependence functionally distinguishes intermediate neural progenitors from neuroblasts in the Drosophila larval brain, balancing neurogenesis with stem cell maintenance...
  70. doi Membrane targeting of Bazooka/PAR-3 is mediated by direct binding to phosphoinositide lipids
    Michael P Krahn
    Abteilung Stammzellbiologie, DFG Research Center for Molecular Physiology of the Brain CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, Gottingen, Germany
    Curr Biol 20:636-42. 2010
    ..Our findings reveal a mechanism for the coupling of plasma membrane polarity and cortical polarity...
  71. pmc A spindle-independent cleavage furrow positioning pathway
    Clemens Cabernard
    Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Nature 467:91-4. 2010
    ..This spindle-independent cleavage furrow mechanism may be relevant to other highly polarized mitotic cells, such as mammalian neural progenitors...
  72. doi Interplay between the transcription factor Zif and aPKC regulates neuroblast polarity and self-renewal
    Kai Chen Chang
    Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604
    Dev Cell 19:778-85. 2010
    ..Thus, reciprocal repression between Zif and aPKC act as a critical regulatory mechanism for establishing cell polarity and controlling neuroblast self-renewal...
  73. doi Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development
    Wei Wang
    School of Life Sciences, Tsinghua University, Beijing, China
    Dev Biol 350:414-28. 2011
    ....
  74. pmc Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6
    Javier Morante
    Center for Developmental Genetics, Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA
    Development 138:687-93. 2011
    ..These defects in cell migration among medulla cortex cells can be rescued by removing DE-Cadherin. Thus, eyeless links neurogenesis and neuronal migration...
  75. doi The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila
    Beatriz P San-Juán
    Centro de Biología Molecular Severo Ochoa Consejo Superior Investigaciones Científicas, Universidad Autonoma de Madrid, Nicolas Cabrera 1, 28049, Madrid, Spain
    Dev Biol 352:70-82. 2011
    ..We show that in addition to dpn, Notch signaling must be regulating other genes during this process that act redundantly with dpn...
  76. pmc Dronc caspase exerts a non-apoptotic function to restrain phospho-Numb-induced ectopic neuroblast formation in Drosophila
    Yingshi Ouyang
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Development 138:2185-96. 2011
    ..Reduction of Dronc activity facilitates ENF induced by phospho-Numb. Our findings uncover a molecular mechanism that regulates Numb activity and suggest a novel role for Dronc caspase in regulating neural stem cell homeostasis...
  77. pmc Functional genomics identifies neural stem cell sub-type expression profiles and genes regulating neuroblast homeostasis
    Travis D Carney
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
    Dev Biol 361:137-46. 2012
    ..These genes are excellent candidates for regulating neural progenitor self-renewal in Drosophila and mammals...
  78. pmc klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division
    Qi Xiao
    Department of Cell and Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Development 139:2670-80. 2012
    ..These results led us to propose that multiple mechanisms converge to exert precise control of klu and distinguish a progenitor cell from its sibling stem cell during asymmetric neuroblast division...
  79. pmc The Osa-containing SWI/SNF chromatin-remodeling complex regulates stem cell commitment in the adult Drosophila intestine
    Xiankun Zeng
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    Development 140:3532-40. 2013
    ..Our data uncover a unique mechanism whereby the commitment of stem cells to discrete lineages is coordinately regulated by chromatin-remodeling factors. ..
  80. pmc Epithelial polarity and spindle orientation: intersecting pathways
    Dan T Bergstralh
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Philos Trans R Soc Lond B Biol Sci 368:20130291. 2013
    ..In this review, we examine several factors implicated in both processes, namely Canoe, Bazooka, aPKC and Discs large, and consider the implications of this work on how the spindle is oriented during epithelial cell divisions. ..
  81. ncbi Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization
    F Yu
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Cell 100:399-409. 2000
    ....
  82. ncbi DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
    M Petronczki
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Nat Cell Biol 3:43-9. 2001
    ..in DmPAR-6 mutants, asymmetric cell divisions in neuroblasts are misorientated, and the proteins Numb and Miranda do not segregate correctly into the basal daughter cell...
  83. ncbi cdc2 links the Drosophila cell cycle and asymmetric division machineries
    M Tio
    Institute of Molecular and Cell Biology, Singapore
    Nature 409:1063-7. 2001
    ..Our findings link cdc2 with asymmetric divisions, and explain why the asymmetric localization of molecules like Inscuteable show cell-cycle dependence...
  84. ncbi Drosophila pod-1 crosslinks both actin and microtubules and controls the targeting of axons
    Michael E Rothenberg
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, 533 Parnassus Avenue, San Francisco, CA 94143, USA
    Neuron 39:779-91. 2003
    ..Taken together, these results reveal novel activities for pod-1 and show that proper levels of Dpod1, an actin/MT crosslinker, must be maintained in the growth cone for correct axon guidance...
  85. ncbi Scribble protein domain mapping reveals a multistep localization mechanism and domains necessary for establishing cortical polarity
    Roger Albertson
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon 1254, Eugene, OR 97403, USA
    J Cell Sci 117:6061-70. 2004
    ..In addition, we show that the LRR domain is sufficient to target Miranda protein to the neuroblast cortex, but that LRR+PDZ will exclude Miranda from the cortex...
  86. ncbi Lgl, Pins and aPKC regulate neuroblast self-renewal versus differentiation
    Cheng Yu Lee
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon 1254, Eugene, Oregon 97403, USA
    Nature 439:594-8. 2006
    ..We conclude that cortical aPKC kinase activity is a potent inducer of neuroblast self-renewal...
  87. ncbi Polycomb group genes are required for neural stem cell survival in postembryonic neurogenesis of Drosophila
    Bruno Bello
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Development 134:1091-9. 2007
    ..Moreover, together with data on mammalian PcG genes, they imply that repression of aberrant reactivation of Hox genes may be a general and evolutionarily conserved role for PcG genes in CNS development...
  88. pmc Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila
    C P Shen
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0725 USA
    Genes Dev 12:1837-46. 1998
    ..The localization of Prospero requires the function of inscuteable and miranda, whereas prospero RNA localization requires inscuteable and staufen function...
  89. ncbi Cell polarity: the PARty expands
    C Q Doe
    Institute of Molecular Biology and the Institute of Neuroscience, HHMI, University of Oregon, Eugene, Oregon 97403, USA
    Nat Cell Biol 3:E7-9. 2001
    ..This common cell-polarity mechanism is used in cell-specific ways, as highlighted by the recent finding that at least two different types of asymmetric division are observed in Drosophila neural precursors...
  90. ncbi Asymmetric cell division: lessons from flies and worms
    B Lu
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143 0725, USA
    Curr Opin Genet Dev 8:392-9. 1998
    ..elegans and Frizzled signaling in Drosophila reveals additional mechanisms for modulating cellular polarity and suggests that there are some similarities between the two systems...
  91. ncbi Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization
    J A Knoblich
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco 94143 0725, USA
    Curr Biol 9:155-8. 1999
    ..the mitotic spindle and for the asymmetric segregation of the proteins Numb [3] [4] [5], Prospero [5] [6] [7] and Miranda [8] [9] into the basal daughter cell...
  92. ncbi Asymmetric cell division: plane but not simple
    P N Adler
    University of Virginia, Department of Biology and Cancer Center, Charlottesville, Virginia 22903, USA
    Curr Biol 11:R233-6. 2001
    ..The orientation of these divisions is under the control of the Frizzled pathway that generates planar polarity in a number of cell types...
  93. ncbi The Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila
    S I Ashraf
    Program in Molecular Medicine and Department of Cell Biology, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Development 128:4757-67. 2001
    ..is significantly lowered, while the expression of other genes that participate in asymmetric division, including miranda, staufen and prospero, appears normal...
  94. ncbi Myosins motor Miranda
    Daniel P Kiehart
    Developmental, Cell and Molecular Biology Group, Department of Biology, Duke University, Durham, NC 27708, USA
    Mol Cell 12:1346-7. 2003
    ..New evidence shows that myosin motors drive the spatial segregation of cell fate determinants during asymmetric cell division. How they do so remains a mystery...
  95. ncbi Drosophila Ric-8 is essential for plasma-membrane localization of heterotrimeric G proteins
    Bernhard Hampoelz
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Nat Cell Biol 7:1099-105. 2005
    ..Our results indicate a model in which both receptor-dependent and receptor-independent G-protein functions are executed at the plasma membrane and require the Ric-8 protein...
  96. ncbi Playing ping pong with pins: cortical and microtubule-induced polarity
    Julie Ahringer
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1184-6. 2005
    ..In this issue of Cell, it is turned around and shown that the transfer of polarity information between the cortex and the spindle is not just one way. In Drosophila neuroblasts, the spindle also has polarizing activity on the cortex...
  97. pmc A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions
    Cathy Slack
    Temasek Lifesciences Laboratory, The National University of Singapore, 117604, Singapore
    BMC Genet 7:33. 2006
    ..The cell fate determinant Prospero and its adapter protein Miranda are asymmetrically localized to the basal cortex of the dividing neuroblast and segregated into the GMC upon ..
  98. ncbi Expression of Drosophila MAGE gene encoding a necdin homologous protein in postembryonic neurogenesis
    Isao Nishimura
    Laboratory of Regulation of Neuronal Development, Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Gene Expr Patterns 7:244-51. 2007
    ..MAGE was also expressed in postmitotic neurons including mushroom body neurons and retinal photoreceptors in adulthood. These results indicate that MAGE expression lasts throughout the postembryonic neurogenesis in Drosophila...
  99. ncbi Asymmetric localisation of Miranda and its cargo proteins during neuroblast division requires the anaphase-promoting complex/cyclosome
    Cathy Slack
    MRC Centre for Developmental Neurobiology, New Hunt s House, King s College London, Guy s Campus, London SE1 1UL, UK
    Development 134:3781-7. 2007
    ..Attenuation of APC/C activity disrupts the asymmetric localisation of the adapter protein Miranda and its associated cargo proteins Staufen, Prospero and Brat, but not other components of the asymmetric division ..
  100. pmc Regulation of glia number in Drosophila by Rap/Fzr, an activator of the anaphase-promoting complex, and Loco, an RGS protein
    Margarita E Kaplow
    Department of Biology, City College and The Graduate Center, City University of New York, New York 10031, USA
    Genetics 178:2003-16. 2008
    ..We propose that Rap/Fzr targets Loco for ubiquitination, thereby regulating glial differentiation in the developing nervous system...
  101. pmc Putting the model to the test: are APC proteins essential for neuronal polarity, axon outgrowth, and axon targeting?
    Nasser M Rusan
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 183:203-12. 2008
    ..These data suggest that proposed cytoskeletal roles for APCs in mammals should be reassessed using loss of function tools...

Research Grants4

  1. GENETIC ANALYSIS OF PREHAIR MORPHOGENESIS
    Paul Adler; Fiscal Year: 2005
    ....
  2. MOLECULAR GENETIC ANALYSIS OF ASYMMETRIC CELL DIVISIONS
    Chris Doe; Fiscal Year: 2002
    ..2) To characterize miranda, a gene encoding a novel coiled-coil protein required for the asymmetric localization of Prospero in neuroblasts...
  3. MECHANISMS OF NEURAL PATTERNING IN MAMMALIAN FOREBRAIN
    Gordon Fishell; Fiscal Year: 2007
    ..abstract_text> ..
  4. REGIONAL PATTERNING IN THE VENTRAL TELENCEPHALON
    GORDON J FISHELL; Fiscal Year: 2010
    ..If we can understand how this population is generated, we will take the first step towards assessing whether we can use gene or cell therapies to ameliorate the devasting consequences associated with cortical interneuron dysfunction. ..