Scr

Summary

Gene Symbol: Scr
Description: Sex combs reduced
Alias: BG:DS07876.2, CG1030, DmScr, Dmel\CG1030, Msc, SCR, l(3)84Af, scr, CG1030 gene product from transcript CG1030-RB, CG1030-PA, CG1030-PB, Scr-PA, Scr-PB, multi sex combs, multiple sex comb, sex combs reduced, sex-combs reduced
Species: fruit fly

Top Publications

  1. ncbi Posterior migration of the salivary gland requires an intact visceral mesoderm and integrin function
    Pamela L Bradley
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Biol 257:249-62. 2003
  2. ncbi Trans-regulation of thoracic homeotic selector genes of the Antennapedia and bithorax complexes by the trithorax group genes: absent, small, and homeotic discs 1 and 2
    D Lajeunesse
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Mech Dev 53:123-39. 1995
  3. ncbi Homeobox genes and axial patterning
    W McGinnis
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    Cell 68:283-302. 1992
  4. ncbi Organogenesis in Drosophila melanogaster: embryonic salivary gland determination is controlled by homeotic and dorsoventral patterning genes
    S Panzer
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Development 114:49-57. 1992
  5. ncbi Homeotic genes have specific functional roles in the establishment of the Drosophila embryonic peripheral nervous system
    J G Heuer
    Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington 47405
    Development 115:35-47. 1992
  6. pmc A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster
    A M Pattatucci
    Howard Hughes Medical Institute, Indiana University, Bloomington 47405
    Genetics 129:423-41. 1991
  7. ncbi Expression of Abdominal-B homeoproteins in Drosophila embryos
    M Delorenzi
    Zoological Institute, University of Zurich, Switzerland
    Development 108:323-9. 1990
  8. ncbi Homeotic genes regulate the spatial expression of putative growth factors in the visceral mesoderm of Drosophila embryos
    R Reuter
    Howard Hughes Medical Institute, University of Colorado, Boulder 80309 0347
    Development 110:1031-40. 1990
  9. pmc Distribution of the Sex combs reduced gene products in Drosophila melanogaster
    J W Mahaffey
    Department of Biology, Indiana University, Bloomington 47405
    Genetics 117:51-60. 1987
  10. pmc Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila
    J A Kennison
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 85:8136-40. 1988

Scientific Experts

Detail Information

Publications126 found, 100 shown here

  1. ncbi Posterior migration of the salivary gland requires an intact visceral mesoderm and integrin function
    Pamela L Bradley
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Biol 257:249-62. 2003
    ..These findings suggest that salivary tube dimensions may be an intrinsic property of salivary gland cells...
  2. ncbi Trans-regulation of thoracic homeotic selector genes of the Antennapedia and bithorax complexes by the trithorax group genes: absent, small, and homeotic discs 1 and 2
    D Lajeunesse
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Mech Dev 53:123-39. 1995
    ..Hypomorphic ash1 mutations cause variegated expression of Antennapedia, Sex combs reduced, Ultrabithorax, and engrailed...
  3. ncbi Homeobox genes and axial patterning
    W McGinnis
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    Cell 68:283-302. 1992
  4. ncbi Organogenesis in Drosophila melanogaster: embryonic salivary gland determination is controlled by homeotic and dorsoventral patterning genes
    S Panzer
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Development 114:49-57. 1992
    ..epithelial cells derived from parasegment 2, is established by the positive action of the homeotic gene Sex combs reduced (Scr)...
  5. ncbi Homeotic genes have specific functional roles in the establishment of the Drosophila embryonic peripheral nervous system
    J G Heuer
    Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington 47405
    Development 115:35-47. 1992
    ..The PNS was examined in embryos homozygous for mutations in the homeotic genes Sex combs reduced (Scr), Antennapedia (Antp), Ultrabithorax (Ubx), abdominal-A (abd-A) and Abdominal-B (Abd-B) with antibodies ..
  6. pmc A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster
    A M Pattatucci
    Howard Hughes Medical Institute, Indiana University, Bloomington 47405
    Genetics 129:423-41. 1991
    We have undertaken a developmental genetic analysis of the homeotic gene Sex combs reduced (Scr) of Drosophila melanogaster by examining embryonic and adult phenotypes of mutations affecting Scr gene function...
  7. ncbi Expression of Abdominal-B homeoproteins in Drosophila embryos
    M Delorenzi
    Zoological Institute, University of Zurich, Switzerland
    Development 108:323-9. 1990
    ..Moreover, an antibody-positive Abd-B mutant suggests that these differ, in the case of m protein expression, to some extent in individual germ layers...
  8. ncbi Homeotic genes regulate the spatial expression of putative growth factors in the visceral mesoderm of Drosophila embryos
    R Reuter
    Howard Hughes Medical Institute, University of Colorado, Boulder 80309 0347
    Development 110:1031-40. 1990
    ..The two growth factor genes therefore are excellent candidates for target genes that are directly regulated by the homeotic genes...
  9. pmc Distribution of the Sex combs reduced gene products in Drosophila melanogaster
    J W Mahaffey
    Department of Biology, Indiana University, Bloomington 47405
    Genetics 117:51-60. 1987
    The spatial and temporal distribution of RNA and protein encoded by the homeotic Sex combs reduced (Scr) gene were examined during Drosophila development...
  10. pmc Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila
    J A Kennison
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 85:8136-40. 1988
    ..e., Sex combs reduced (Scr), Brista (Ba), trithorax (trx), Polycomb (Pc), Polycomblike (Pcl), and Sex comb on midleg (Scm)]...
  11. pmc Genes controlling segmental specification in the Drosophila thorax
    G Struhl
    Proc Natl Acad Sci U S A 79:7380-4. 1982
    The roles of three homeotic genes, Ubx+, Scr+, and Antp+, in the Drosophila thorax have been studied by determining the cellular phenotypes of mutations resulting in loss of gene function...
  12. ncbi Homeotic response elements are tightly linked to tissue-specific elements in a transcriptional enhancer of the teashirt gene
    A McCormick
    Department of Developmental Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, CA 94305 5427, USA
    Development 121:2799-812. 1995
    ..We propose that local interactions between homeotic proteins and other factors effect activation of targets in proper cell types...
  13. ncbi Specification of anteroposterior cell fates in Caenorhabditis elegans by Drosophila Hox proteins
    C P Hunter
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0554, USA
    Nature 377:229-32. 1995
    ..Together these findings suggest that factors important for target recognition by specific Hox proteins have been conserved throughout much of the animal kingdom...
  14. pmc Characterization of the cis-regulatory region of the Drosophila homeotic gene Sex combs reduced
    J G Gindhart
    Howard Hughes Medical Institute, Indiana University, Bloomington 47405, USA
    Genetics 139:781-95. 1995
    The Drosophila homeotic gene Sex combs reduced (Scr) controls the segmental identity of the labial and prothoracic segments in the embryo and adult...
  15. pmc Setting limits on homeotic gene function: restraint of Sex combs reduced activity by teashirt and other homeotic genes
    D J Andrew
    Department of Developmental Biology, Stanford University School of Medicine, CA 94305 5427
    EMBO J 13:1132-44. 1994
    ..We have identified genes that limit where the homeotic gene Sex combs reduced (Scr) can affect cell fates in the Drosophila embryo...
  16. ncbi The mouse Hox-1.3 gene is functionally equivalent to the Drosophila Sex combs reduced gene
    J J Zhao
    Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029 6574
    Genes Dev 7:343-54. 1993
    To test whether the mouse Hox-1.3 gene is a cognate of the Drosophila Sex combs reduced (Scr) gene, we inserted a hsp 70-Hox-1.3 fusion gene into the Drosophila genome...
  17. ncbi Tubulogenesis in Drosophila: a requirement for the trachealess gene product
    D D Isaac
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2196, USA
    Genes Dev 10:103-17. 1996
    ..trh expression in the salivary duct is controlled by the homeotic gene, Sex combs reduced (Scr), and by another salivary gland gene, fork head (fkh)...
  18. ncbi Extra specificity from extradenticle: the partnership between HOX and PBX/EXD homeodomain proteins
    R S Mann
    Department of Biochemistry and Molecular Biophysics, Columbia University, NY 10032, USA
    Trends Genet 12:258-62. 1996
    ....
  19. ncbi The role of HOM-C genes in segmental transformations: reexamination of the Drosophila Sex combs reduced embryonic phenotype
    J D Pederson
    Department of Genetics, North Carolina State University, Raleigh 27695 7614, USA
    Dev Biol 180:131-42. 1996
    ..Complex of Drosophila specify identity of the posterior head segments; the labial segment requires Sex combs reduced (Scr) for proper development, Deformed (Dfd) specifies maxillary and mandibular identity, and labial is ..
  20. ncbi The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle
    D J Andrew
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2196, USA
    Development 124:181-93. 1997
    ..The lateralizing phenotype of dCREB-A mutants reveals a much broader function for CREB proteins than previously thought...
  21. ncbi Muscles in the Drosophila second thoracic segment are patterned independently of autonomous homeotic gene function
    S Roy
    National Centre for Biological Sciences, Tata Institute of Fundamental Research, P O Box 1234, IISc Campus, Bangalore, 560012, India
    Curr Biol 7:222-7. 1997
    ..Here, we have examined the roles of homeotic genes in the patterning of the somatic muscles of the thoracic segments of Drosophila...
  22. ncbi Nuclear translocation of extradenticle requires homothorax, which encodes an extradenticle-related homeodomain protein
    G E Rieckhof
    Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Cell 91:171-83. 1997
    ..These data suggest a novel and evolutionarily conserved mechanism for regulating HOX activity in which a direct protein-protein interaction between EXD and HTH results in EXD's nuclear translocation...
  23. ncbi Control of antennal versus leg development in Drosophila
    F Casares
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Nature 392:723-6. 1998
    ..Taken together, these data indicate that hth is an antennal selector gene, and that Antp promotes leg development by repressing hth and consequently nuclear Exd...
  24. pmc Activity regulation of a Hox protein and a role for the homeodomain in inhibiting transcriptional activation
    X Li
    Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    EMBO J 18:198-211. 1999
    ..The regulation of Hox transcriptional activation functions may underlie the different functional specificities of proteins belonging to this developmental patterning family...
  25. ncbi Structure of a DNA-bound Ultrabithorax-Extradenticle homeodomain complex
    J M Passner
    Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nature 397:714-9. 1999
    ..Together, these protein-DNA and protein-protein interactions define the general principles by which homeotic proteins interact with Extradenticle (or Pbx1) to affect development along the anterior-posterior axis of animals...
  26. pmc WRS-85D: A tryptophanyl-tRNA synthetase expressed to high levels in the developing Drosophila salivary gland
    P Seshaiah
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2196, USA
    Mol Biol Cell 10:1595-608. 1999
    ..WRS-85D expression is dependent on the homeotic gene Sex combs reduced (Scr)...
  27. pmc The control of trunk Hox specificity and activity by Extradenticle
    H D Ryoo
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Genes Dev 13:1704-16. 1999
    ..a 37-bp element (fkh[250]) derived from the fork head (fkh) gene, a natural target of the Hox gene Sex combs reduced (Scr)...
  28. ncbi Regulation and function of Scr, exd, and hth in the Drosophila salivary gland
    K D Henderson
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205 2196, USA
    Dev Biol 217:362-74. 2000
    Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr)...
  29. pmc Phosphorylation status of the SCR homeodomain determines its functional activity: essential role for protein phosphatase 2A,B'
    M Berry
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:2946-57. 2000
    b>Sex combs reduced (SCR) is a Drosophila Hox protein that determines the identity of the labial and prothoracic segments...
  30. pmc Transvection and silencing of the Scr homeotic gene of Drosophila melanogaster
    Jeffrey W Southworth
    Section on Drosophila Gene Regulation, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2785, USA
    Genetics 161:733-46. 2002
    The Sex combs reduced (Scr) gene specifies the identities of the labial and first thoracic segments in Drosophila melanogaster...
  31. pmc Tube morphogenesis: closure, but many openings remain
    W James Nelson
    Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
    Trends Cell Biol 13:615-21. 2003
    ..This review provides a summary of these pathways based upon articles published in the Tube Morphogenesis Series in Trends in Cell Biology...
  32. pmc MicroRNA targets in Drosophila
    Anton J Enright
    Computational Biology Center, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Genome Biol 5:R1. 2003
    ..Application to the D. melanogaster, Drosophila pseudoobscura and Anopheles gambiae genomes identifies several hundred target genes potentially regulated by one or more known miRNAs...
  33. ncbi Function of the Trithorax-like gene during Drosophila development
    Fernando Bejarano
    Centro de Biologia Molecular, CSIC UAM, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Dev Biol 268:327-41. 2004
    ..We also suggest that GAGA factor may not have a dual activator/repressor function. Rather, Trithorax-like mutations may produce dual loss of activation and loss of repression effects...
  34. ncbi Analysis of Drosophila proboscipedia mutant alleles
    I Tayyab
    Department of Biology, University of Western Ontario, London, Canada
    Genome 47:600-9. 2004
    ..missense alleles suggests that the PB HD is required for maxillary palp and proboscis development and pb - Sex combs reduced (Scr) genetic interaction...
  35. ncbi Multiplex detection of RNA expression in Drosophila embryos
    Dave Kosman
    Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA
    Science 305:846. 2004
    ....
  36. pmc Principles of microRNA-target recognition
    Julius Brennecke
    European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 3:e85. 2005
    ....
  37. ncbi Recognition of distinct target sites by a unique Labial/Extradenticle/Homothorax complex
    Andreas Ebner
    Biozentrum der Universitat Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Development 132:1591-600. 2005
    ..Thus Lab, and most probably other Hox proteins, selects different DNA sequences in regulating downstream target genes. These observations have implications with regard to the current model of Hox-binding selectivity...
  38. ncbi Evolutionarily conserved domains required for activation and repression functions of the Drosophila Hox protein Ultrabithorax
    Ella Tour
    Section in Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Development 132:5271-81. 2005
    ..at the N termini of many Hox proteins, and is conserved in the activation domain of another Hox protein, Sex combs reduced. These results suggest that the N-terminal region containing the SSYF motif has been conserved in many Hox ..
  39. ncbi Differential Delta expression underlies the diversity of sensory organ patterns among the legs of the Drosophila adult
    Stuti Shroff
    Department of Biological Sciences, University of Illinois at Chicago, 900 S Ashland Avenue, Chicago, IL 60607, USA
    Mech Dev 124:43-58. 2007
    ..Our findings suggest that the Hox genes, Sex combs reduced (Scr) and Ultrabithorax (Ubx), establish differential expression of the proneural gene achaete (ac) by ..
  40. ncbi The rise and fall of Hox gene clusters
    Denis Duboule
    National Research Centre Frontiers in Genetics, Department of Zoology and Animal Biology, University of Geneva, Sciences III, Switzerland
    Development 134:2549-60. 2007
    ..By integrating these parameters, general conclusions emerge that can help solve the aforementioned dilemma...
  41. pmc Comparing active and repressed expression states of genes controlled by the Polycomb/Trithorax group proteins
    Christian Beisel
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 104:16615-20. 2007
    ..Besides its presumptive function in recruiting PcG complexes to their site of action, our results now uncover that Pho plays an additional role in the repression of already induced genes...
  42. pmc Functional specificity of a Hox protein mediated by the recognition of minor groove structure
    Rohit Joshi
    Department of Biochemistry and Molecular Biophysics, Columbia University, HHSC 1104, New York, NY 10032, USA
    Cell 131:530-43. 2007
    ..Our results suggest that these residues, which are conserved in a paralog-specific manner, confer specificity by recognizing a sequence-dependent DNA structure instead of directly reading a specific DNA sequence...
  43. pmc Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    Alexander Stark
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
    Genome Res 17:1865-79. 2007
    ..For mir-10 in particular, both arms show abundant processing, and both show highly conserved target sites in Hox genes, suggesting a possible cooperation of the two arms, and their role as a master Hox regulator...
  44. doi How Drosophila change their combs: the Hox gene Sex combs reduced and sex comb variation among Sophophora species
    Neel B Randsholt
    CNRS, Centre de Genetique Moleculaire, UPR 2167, Gif sur Yvette F 91190, France
    Evol Dev 10:121-33. 2008
    ..All contribute to spatial regulation of the Hox gene Sex combs reduced (Scr), which is crucial for comb formation...
  45. doi The YPWM motif links Antennapedia to the basal transcriptional machinery
    Frederic Prince
    Biozentrum, University of Basel, Klingelberstrasse 70, CH 4056 Basel, Switzerland
    Development 135:1669-79. 2008
    ..BIP2 is a TATA-binding protein associated factor (also known as dTAFII3) that links ANTP to the basal transcriptional machinery...
  46. pmc Analysis of the sequence and phenotype of Drosophila Sex combs reduced alleles reveals potential functions of conserved protein motifs of the Sex combs reduced protein
    Lovesha Sivanantharajah
    Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada
    Genetics 182:191-203. 2009
    The Drosophila Hox gene, Sex combs reduced (Scr), is required for patterning the larval and adult, labial and prothoracic segments. Fifteen Scr alleles were sequenced and the phenotypes analyzed in detail...
  47. doi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..Polycomb repression appears to be a critical function of Sxc/Ogt in Drosophila and may be mediated by the glycosylation of Polyhomeotic...
  48. pmc Visualization of individual Scr mRNAs during Drosophila embryogenesis yields evidence for transcriptional bursting
    Adam Pare
    Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Curr Biol 19:2037-42. 2009
    ..We use this method to characterize transcription at the endogenous locus of the Hox gene Sex combs reduced (Scr), by comparing a stably expressing group of cells to a group that only transiently expresses the gene...
  49. pmc Function and specificity of synthetic Hox transcription factors in vivo
    Dimitrios K Papadopoulos
    Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 107:4087-92. 2010
    ..Here we show that synthetic Sex combs reduced (Scr) genes that encode the Scr C terminus containing the homedomain (HD) and YPWM motif (Scr-HD) are ..
  50. pmc The H3K27me3 demethylase dUTX is a suppressor of Notch- and Rb-dependent tumors in Drosophila
    Hans Martin Herz
    M D Anderson Cancer Center, Department of Biochemistry and Molecular Biology, Graduate Program in Genes and Development, 1515 Holcombe Boulevard, Unit 1000, Houston, TX 77030, USA
    Mol Cell Biol 30:2485-97. 2010
    ..In summary, these data suggest that dUTX is a suppressor of Notch- and Rbf-dependent tumors in Drosophila melanogaster and may provide a model for UTX-dependent tumorigenesis in humans...
  51. pmc Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements
    Juli D Uhl
    Division of Developmental Biology, Cincinnati Children s Hospital, 3333 Burnet Ave, MLC 7007, Cincinnati, OH 45229, USA
    Dev Biol 343:154-66. 2010
    ..Based upon these findings, we suggest a general set of guidelines to serve as a basis for designing bioinformatics algorithms aimed at identifying Hox regulatory elements using the wealth of recently sequenced genomes...
  52. pmc Dissecting the functional specificities of two Hox proteins
    Rohit Joshi
    Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, New York 10032, USA
    Genes Dev 24:1533-45. 2010
    ..For the Drosophila Hox protein Sex combs reduced (Scr), Exd has been shown to position a normally unstructured portion of Scr so that two basic amino acid ..
  53. pmc Functional synthetic Antennapedia genes and the dual roles of YPWM motif and linker size in transcriptional activation and repression
    Dimitrios K Papadopoulos
    Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 108:11959-64. 2011
    ....
  54. pmc Competition for cofactor-dependent DNA binding underlies Hox phenotypic suppression
    Barbara Noro
    Department of Neuroscience, College of Physicians and Surgeons, Columbia University, New York 10032, USA
    Genes Dev 25:2327-32. 2011
    ..Together, these results suggest that Hox-specific motifs endow posterior Hox proteins with the ability to dominate over more anterior ones via a cofactor-dependent DNA-binding mechanism...
  55. pmc Cofactor binding evokes latent differences in DNA binding specificity between Hox proteins
    Matthew Slattery
    Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168 th Street, HHSC 1104, New York, NY 10032, USA
    Cell 147:1270-82. 2011
    ..Together, these data suggest that emergent DNA recognition properties revealed by interactions with cofactors contribute to transcription factor specificities in vivo...
  56. pmc Variable motif utilization in homeotic selector (Hox)-cofactor complex formation controls specificity
    Katherine M Lelli
    Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:21122-7. 2011
    ..We find that Sex combs reduced (Scr) uses a simple interaction mechanism, where a single tryptophan-containing motif is necessary for Exd-..
  57. doi Dimer formation via the homeodomain is required for function and specificity of Sex combs reduced in Drosophila
    Dimitrios K Papadopoulos
    Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland
    Dev Biol 367:78-89. 2012
    ..We have unexpectedly found that the Drosophila Sex combs reduced (Scr) protein dimerizes in vivo via the homeodomain, whereas its closest relative, Antennapedia (Antp), does ..
  58. doi A histone mutant reproduces the phenotype caused by loss of histone-modifying factor Polycomb
    Ana Raquel Pengelly
    Chromatin and Chromosome Biology Research Group, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Science 339:698-9. 2013
    ..Taken together, these analyses demonstrate that H3-K27 is the crucial physiological substrate that PRC2 modifies for Polycomb repression...
  59. pmc The homeotic gene Sex combs reduced of Drosophila melanogaster is differentially regulated in the embryonic and imaginal stages of development
    A M Pattatucci
    Howard Hughes Medical Institute, Indiana University, Bloomington 47405
    Genetics 129:443-61. 1991
    The Sex combs reduced (Scr) locus is unique among the genes contained within the Antennapedia complex (ANT-C) of Drosophila melanogaster in that it directs functions that are required for both cephalic and thoracic development in the ..
  60. ncbi Why are Hox genes clustered?
    R S Mann
    Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Bioessays 19:661-4. 1997
    ..Moreover, characterizing the activity of one of these mouse enhancers in Drosophila illustrates that despite many similarities, not all Hox clusters are built in the same way...
  61. ncbi Tarsus determination in Drosophila melanogaster
    Anthony Percival-Smith
    Department of Biology, University of Western Ontario, London, Canada
    Genome 48:712-21. 2005
    ..whether Antennapedia (ANTP) cell autonomously or noncell autonomously determines tarsus identity and whether Sex combs reduced (SCR) is the HOX protein required for normal tarsus determination...
  62. pmc DSP1, an HMG-like protein, is involved in the regulation of homeotic genes
    M Decoville
    Centre de Biophysique Moleculaire, CNRS UPR 4301, conventionnée avec l Université d Orléans, 45071 Orleans, France
    Genetics 157:237-44. 2001
    ..causes homeotic transformations resembling those associated with loss of function in the homeotic genes Sex combs reduced (Scr), Ultrabithorax (Ubx), and Abdominal-B...
  63. ncbi DSP1, a Drosophila HMG protein, is involved in spatiotemporal expression of the homoeotic gene Sex combs reduced
    Aurélien Rappailles
    Centre de Biophysique Moleculaire, CNRS UPR 4301, conventionnée avec l Université d Orléans, rue C Sadron, 45071 Orleans, France
    Biol Cell 97:779-85. 2005
    ..The homoeotic gene Scr (Sex combs reduced) contained in the Antennapedia complex specifies segmental identity of the labial and prothoracic segments ..
  64. ncbi Sex-specific expression of a HOX gene associated with rapid morphological evolution
    Olga Barmina
    Section of Evolution and Ecology, University of California Davis, One Shields Ave, Davis, CA 95616, USA
    Dev Biol 311:277-86. 2007
    ..Here, we show that sex-specific regulation of the HOX gene Sex combs reduced (Scr) is associated with the origin and evolution of the Drosophila sex comb - a novel and rapidly ..
  65. pmc Functional characterization of transcription factor motifs using cross-species comparison across large evolutionary distances
    Jaebum Kim
    Department of Computer Science, University of Illinois at Urbana Champaign, Urbana, Illinois, United States of America
    PLoS Comput Biol 6:e1000652. 2010
    ..We also apply the framework to find motifs associated with socially regulated gene sets in the honeybee, Apis mellifera, using comparisons with Nasonia, a solitary species, to identify honeybee-specific associations...
  66. doi Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex
    Swarnava Roy
    NIDDK Metabolic Diseases Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Genet Genomics 285:113-23. 2011
    ..A control gene, Dref, was not affected. A full understanding of the regulation of ANT-C genes during development will have to take the role of BEAF into account...
  67. ncbi In vivo analysis of the helix-turn-helix motif of the fushi tarazu homeo domain of Drosophila melanogaster
    K Furukubo-Tokunaga
    Department of Cell Biology, University of Basel, Swtizerland
    Genes Dev 6:1082-96. 1992
    ..We started out by testing the function of chimeric ftz proteins containing either a part of the Sex combs reduced (Scr) or the muscle segment homeobox (msh) HDs...
  68. pmc Genetic analysis of the enhancer of zeste locus and its role in gene regulation in Drosophila melanogaster
    R S Jones
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138 2097
    Genetics 126:185-99. 1990
    ..We propose that the E(z) loss-of-function eye color and homeotic phenotypes may both be due to gene derepression, and that the E(z)+ product may be a general repressing factor required for both examples of negative gene regulation...
  69. ncbi What determines the specificity of action of Drosophila homeodomain proteins?
    S Hayashi
    National Institute of Genetics, Shizuoka ken, Japan
    Cell 63:883-94. 1990
  70. ncbi A new Drosophila homeo box gene is expressed in mesodermal precursor cells of distinct muscles during embryogenesis
    C Dohrmann
    Max Planck Institut fur Entwicklungsbiologie, Abteilung III Genetik, Tubingen, Germany
    Genes Dev 4:2098-111. 1990
    ..In addition to the mesoderm, S59 is expressed in a subset of neuronal cells of the CNS and their precursors and also in cells of a small region of the midgut...
  71. ncbi Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network
    G Frigerio
    Cell 47:735-46. 1986
    ..The observed linking of gene sets through combinations of homologies coding for protein domains is consistent with a general network concept of gene action...
  72. pmc Polycomblike: a gene that appears to be required for the normal expression of the bithorax and antennapedia gene complexes of Drosophila melanogaster
    I M Duncan
    Genetics 102:49-70. 1982
    ....
  73. pmc Identification of homeotic target genes in Drosophila melanogaster including nervy, a proto-oncogene homologue
    P G Feinstein
    Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Genetics 140:573-86. 1995
    ..These results illustrate potentially general rules about the homeotic control of target gene expression and suggest that subtractive hybridization can be used to isolate interesting homeotic target genes...
  74. pmc Discovery and genetic definition of the Drosophila Antennapedia complex
    R Denell
    Division of Biology, Kansas State University, Manhattan 66506 4901
    Genetics 138:549-52. 1994
  75. ncbi Homeotic genes and positional signalling in the Drosophila viscera
    M Bienz
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Trends Genet 10:22-6. 1994
    ..The homeotic genes that are expressed in the viscera control morphogenesis and differentiation of the larval midgut...
  76. pmc Genomic regions required for morphogenesis of the Drosophila embryonic midgut
    D Bilder
    Department of Developmental Biology, Stanford University School of Medicine, California 94305 5427, USA
    Genetics 141:1087-100. 1995
    ..Another genomic region is required to activate transcription of the homeotic genes Antp and Scr specifically in visceral mesoderm...
  77. ncbi Nuclear organization and gene expression: homologous pairing and long-range interactions
    S Henikoff
    Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA
    Curr Opin Cell Biol 9:388-95. 1997
    ..Recent work has revealed that such influences are widespread in eukaryotes and that chromosome architecture is likely to be of fundamental importance for nuclear structure and function...
  78. ncbi Involvement of the Bombyx Scr gene in development of the embryonic silk gland
    H Kokubo
    National Institute for Basic Biology, Myodaiji cho, Okazaki, Japan
    Dev Biol 186:46-57. 1997
    ..that encode the homeodomain and its flanking regions identical to the corresponding regions of Deformed and Sex combs reduced. Using in situ hybridization and immunohistochemistry, we analyzed the expression patterns of these genes ..
  79. ncbi A sequence conserved in vertebrate Hox gene introns functions as an enhancer regulated by posterior homeotic genes in Drosophila imaginal discs
    L P Keegan
    Department of Cell Biology, Basel University Biozentrum, Switzerland
    Mech Dev 63:145-57. 1997
    ..Testing a reporter gene construct with mutated binding sites in mouse embryos shows that HB1 is also active in the expression domains of posterior Hox genes in the mouse neural tube...
  80. ncbi cDNA sequence and expression pattern of the Drosophila melanogaster PAPS synthetase gene: a new salivary gland marker
    D Jullien
    Laboratoire de Biologie Moleculaire Eucaryote, C N R S U P R 9006, Toulouse, France
    Mech Dev 68:179-86. 1997
    ..We discuss the possibility that the pattern of expression of the DmPAPSS gene might reflect a major role for sulfation in mucus biosynthesis at the end of Drosophila embryogenesis...
  81. pmc Functional and regulatory interactions between Hox and extradenticle genes
    N Azpiazu
    Centro de Biologia Molecular Centro Superior de Investigaciones Cientificas Universidad Autońoma de Madrid, Madrid, Spain
    Genes Dev 12:261-73. 1998
    ..As the Hoxd10 gene has the same properties as Drosophila BX-C genes, we suggest that the control mechanism of subcellular distribution of Exd found in Drosophila probably operates in other organisms as well...
  82. ncbi Polycomb and trithorax group proteins mediate the function of a chromatin insulator
    T I Gerasimova
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Cell 92:511-21. 1998
    ..The results suggest a model in which PcG and TrxG proteins regulate insulator function by establishing higher order domains of chromatin organization required for the assembly of functional insulators at the nuclear matrix...
  83. ncbi Regulation of the Sex combs reduced gene in Drosophila
    J A Kennison
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2785, USA
    Ann N Y Acad Sci 842:28-35. 1998
    The Sex combs reduced gene of the Antennapedia complex specifics the identities of the anterior thoracic and posterior head segments, including the primordium of the larval salivary gland...
  84. ncbi trithorax and the regulation of homeotic gene expression in Drosophila: a historical perspective
    P W Ingham
    Developmental Genetics Programme, The Krebs Institute, University of Sheffield, United Kingdom
    Int J Dev Biol 42:423-9. 1998
    ....
  85. ncbi The Drosophila Pax gene eye gone is required for embryonic salivary duct development
    N A Jones
    Division of Genetics, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Development 125:4163-74. 1998
    ..After the initial establishment of the salivary primordium by Sex combs reduced, fork head excludes eye gone expression from the pregland cells so that its salivary expression is ..
  86. ncbi Hox genes differentially regulate Serrate to generate segment-specific structures
    E L Wiellette
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
    Development 126:1985-95. 1999
    ..However, Serrate does not require rhomboid function in the maxillary segment, suggesting that the Hox-Serrate pathway to segment-specific morphogenesis can be linked to more than one downstream function...
  87. ncbi Selector genes and limb identity in arthropods and vertebrates
    S D Weatherbee
    Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA
    Cell 97:283-6. 1999
  88. pmc The lawc gene is a new member of the trithorax-group that affects the function of the gypsy insulator of Drosophila
    I D Zorin
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genetics 152:1045-55. 1999
    ..These results suggest that the lawc protein is involved in regulating the higher-order organization of chromatin...
  89. ncbi A common mechanism for antenna-to-Leg transformation in Drosophila: suppression of homothorax transcription by four HOM-C genes
    L C Yao
    Institute of Genetics, Department of Life Science, National Yang Ming University, Shipai, Taipei, 112, Taiwan, Republic of China
    Dev Biol 211:268-76. 1999
    ..Here we show that the Scr, Antp, Ubx, and abd-A HOM-C genes all exert their effects through a common mechanism: suppressing the transcription ..
  90. ncbi Organ shape in the Drosophila salivary gland is controlled by regulated, sequential internalization of the primordia
    M M Myat
    Department of Cell Biology and Anatomy, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205 2196, USA
    Development 127:679-91. 2000
    ..We propose that fas is required for hkb-dependent signaling events that coordinate internalization...
  91. pmc Molecular basis for the inhibition of Drosophila eye development by Antennapedia
    S Plaza
    Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    EMBO J 20:802-11. 2001
    ..Using both in vitro and in vivo experiments, we demonstrate that this inhibitory mechanism involves direct protein-protein interactions between the DNA-binding domains of EY and ANTP, resulting in mutual inhibition...
  92. ncbi The domino gene of Drosophila encodes novel members of the SWI2/SNF2 family of DNA-dependent ATPases, which contribute to the silencing of homeotic genes
    M L Ruhf
    UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, France
    Development 128:1429-41. 2001
    ..Altogether, the data lead us to propose that domino acts as a repressor by interfering with chromatin structure. This activity is likely to be performed as a subunit of a chromatin-remodeling complex...
  93. ncbi The ground state of the ventral appendage in Drosophila
    F Casares
    Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168 Street, New York, NY 10032, USA
    Science 293:1477-80. 2001
    ..These results raise the possibility that, during evolution, leglike appendages could have developed without selector gene activity...
  94. ncbi The Drosophila proboscis is specified by two Hox genes, proboscipedia and Sex combs reduced, via repression of leg and antennal appendage genes
    A Abzhanov
    Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Development 128:2803-14. 2001
    ..Here we demonstrate that the homeotic gene products Proboscipedia (Pb) and Sex combs reduced (Scr) regulate the limb genes in the labial disc to give rise to a unique type of appendage, the proboscis...
  95. ncbi Functional equivalence of Hox gene products in the specification of the tritocerebrum during embryonic brain development of Drosophila
    F Hirth
    Institute of Zoology, Biocenter Pharmacenter, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Development 128:4781-8. 2001
    ..This suggests that in embryonic brain development, differences in Hox gene action rely mainly on cis-acting regulatory elements and not on Hox protein specificity...
  96. ncbi The evolution of HOM-C homeoboxes in the Dipteran family Drosophilidae
    R DeSalle
    Division of Invertebrates, American Museum of Natural History, 79th Street at Central Park West, New York, NY 10024, USA
    Insect Mol Biol 12:345-51. 2003
    ..These new homeoboxes are from the labial, proboscipedia, Deformed, Sex combs reduced, fushi tarazu, Antennapedia, Ultrabithorax, abdominal-A and Abdominal-B genes...
  97. pmc Drosophila signal peptide peptidase is an essential protease for larval development
    David J Casso
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143, USA
    Genetics 170:139-48. 2005
    ..Flies bearing mutations in conserved residues or carrying deficiencies for the Spp gene had defective tracheae and died as larvae...
  98. pmc A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities
    Tetyana Klymenko
    Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Genes Dev 20:1110-22. 2006
    ..We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state...
  99. ncbi The specification of a highly derived arthropod appendage, the Drosophila labial palps, requires the joint action of selectors and signaling pathways
    Laurent Joulia
    Centre de Biologie du Developpement, CNRS, 118 route de Narbonne, 31062 Toulouse Cedex 04, France
    Dev Genes Evol 216:431-42. 2006
    ..We have examined the roles of the Hox selector genes proboscipedia (pb) and Sex combs reduced (Scr), and the antennal selectors homothorax (hth) and spineless (ss) in labial specification, by tissue-..
  100. ncbi Missing link in the evolution of Hox clusters
    Soichi Ogishima
    Department of Bioinformatics, Graduate School of Tokyo Medical and Dental University Yushima 1 5 45, Tokyo 113 8510, Japan
    Gene 387:21-30. 2007
    ..Our findings suggested that motif gains/diversifications led to the explosive diversity of the bilaterian body plan...
  101. pmc Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control
    Soda Balla Diop
    Institut de Biologie du Developpement de Marseille Luminy, CNRS UMR6216 Université de la Méditerranée, Parc Scientifique de Luminy, Case 907, 13288 Marseille Cedex 9, France
    EMBO Rep 9:260-6. 2008
    ..Furthermore, the enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output...

Research Grants11

  1. DISSECTION OF ONCOGENIC PATHWAYS USING DROSOPHILA
    Richard Mann; Fiscal Year: 2004
    ..The use of Drosophila genetics is proposed to help make connections between genetic functions that cannot be easily identified in mammalian experimental systems. ..
  2. Integrated Approcahes to Find Hox-regulated DNA Elements
    Richard Mann; Fiscal Year: 2008
    ..Once these methods are worked out, they will be applicable to a wide variety of problems in biology. [unreadable] [unreadable]..
  3. SYNAPSE STRUCTURE AND DEVELOPMENT
    W Nelson; Fiscal Year: 2001
    ..This research will aid in the prevention of psychiatric illness and the development of therapies for afflicted individuals. ..
  4. TRANSCRIPTIONAL COACTIVATORS IN ENDOCRINE REGULATION
    Richard Goodman; Fiscal Year: 2003
    ..Finally, we will characterize the ability of the "CREB binding domain" of CBP to interact with other transcription factors activated or inhibited by Ser/Thr phosphorylation. ..
  5. Cytoskeleton Coordination in Neuronal Morphogenesis
    W Nelson; Fiscal Year: 2005
    ....
  6. Genetic Transfer in the Evolution of Infectious Disease
    Rob DeSalle; Fiscal Year: 2005
    ....
  7. Quantitative genomics of sexual dimorphism
    Sergey Nuzhdin; Fiscal Year: 2005
    ..We expect our results to have a profound impact on our understanding of the evolution of sexual dimorphism, and the role that sexual differentiation plays in the generation of phenotypic diversity. ..
  8. RECEPTORS AND SIGNALING IN NEUROENDOCRINE SYSTEMS
    Richard Goodman; Fiscal Year: 2006
    ..Core laboratories for cell culture, protein chemistry, and administration are designed to allow the proposed studies to be performed in an efficient and cost-effective manner. ..
  9. Regulation of Cell Migration by the APC-Microtubule Complex
    William Nelson; Fiscal Year: 2009
    ..abstract_text> ..
  10. Identification of gene targets for morphine and other drugs of abuse
    Richard Goodman; Fiscal Year: 2009
    ..The concept that CREB signaling induces mediators of translational arrest has profound implications for the understanding of drug action. ..
  11. Regulation of MeCP2 by CREB-induced microRNAs
    Richard H Goodman; Fiscal Year: 2010
    ..The studies described in this proposal address a completely novel mechanism of gene regulation that has implications for the understanding of MeCP2 biology and neural development in general. ..