stau

Summary

Gene Symbol: stau
Description: staufen
Alias: CG5753, Dmel\CG5753, Dmstau, STAU, Stau, Stauf, dStau, CG5753 gene product from transcript CG5753-RA, CG5753-PA, CG5753-PB, CG5753-PC, stau-PA, stau-PB, stau-PC, stauffen
Species: fruit fly

Top Publications

  1. ncbi Overexpression of oskar directs ectopic activation of nanos and presumptive pole cell formation in Drosophila embryos
    J L Smith
    Department of Biological Sciences Stanford University, California 94305
    Cell 70:849-59. 1992
  2. ncbi Localization of vasa, a component of Drosophila polar granules, in maternal-effect mutants that alter embryonic anteroposterior polarity
    B Hay
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143
    Development 109:425-33. 1990
  3. ncbi Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres
    D Tautz
    Max Planck Institut fur Entwicklungsbiologie, Arbeitsgruppe Jäckle, Tubingen, FRG
    Nature 332:281-4. 1988
  4. ncbi Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner
    D Ferrandon
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Federal Republic of Germany
    Cell 79:1221-32. 1994
  5. ncbi Dimerization of the 3'UTR of bicoid mRNA involves a two-step mechanism
    C Wagner
    UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Descartes 67084, Strasbourg Cedex, France
    J Mol Biol 313:511-24. 2001
  6. ncbi Localization of bicoid mRNA in late oocytes is maintained by continual active transport
    Timothy T Weil
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Dev Cell 11:251-62. 2006
  7. ncbi Neuronal RNA granules: movers and makers
    Michael A Kiebler
    Center for Brain Research, Medical University of Vienna, A 1090 Vienna, Austria
    Neuron 51:685-90. 2006
  8. doi Membrane targeting of Bazooka/PAR-3 is mediated by direct binding to phosphoinositide lipids
    Michael P Krahn
    Abteilung Stammzellbiologie, DFG Research Center for Molecular Physiology of the Brain CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, Gottingen, Germany
    Curr Biol 20:636-42. 2010
  9. pmc Drosophila Ge-1 promotes P body formation and oskar mRNA localization
    Shih Jung Fan
    Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 6:e20612. 2011
  10. pmc RNA-RNA interaction is required for the formation of specific bicoid mRNA 3' UTR-STAUFEN ribonucleoprotein particles
    D Ferrandon
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    EMBO J 16:1751-8. 1997

Research Grants

  1. GENETIC ANALYSIS OF PREHAIR MORPHOGENESIS
    Paul Adler; Fiscal Year: 2005
  2. ASYMMETRIC CELL DIVISION IN C. ELEGANS EMBRYOS
    Kenneth Kemphues; Fiscal Year: 2005
  3. NEUROLOGICAL FUNCTION OF FRAGILE X GENE IN DROSOPHILA
    Kendal Broadie; Fiscal Year: 2006
  4. Synaptic Mechanisms in Drosophila Neurodegeneration
    Kendal Broadie; Fiscal Year: 2009
  5. GENETIC ANALYSIS OF SYNAPSE FORMATION AND FUNCTION
    Kendal Broadie; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications143 found, 100 shown here

  1. ncbi Overexpression of oskar directs ectopic activation of nanos and presumptive pole cell formation in Drosophila embryos
    J L Smith
    Department of Biological Sciences Stanford University, California 94305
    Cell 70:849-59. 1992
    ..Strikingly, formation of these ectopic pole cells is enhanced in nanos mutants. This observation may reflect competition between nanos and the germ cell determinant for a shared and limiting precursor...
  2. ncbi Localization of vasa, a component of Drosophila polar granules, in maternal-effect mutants that alter embryonic anteroposterior polarity
    B Hay
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143
    Development 109:425-33. 1990
    ....
  3. ncbi Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres
    D Tautz
    Max Planck Institut fur Entwicklungsbiologie, Arbeitsgruppe Jäckle, Tubingen, FRG
    Nature 332:281-4. 1988
    ....
  4. ncbi Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner
    D Ferrandon
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Federal Republic of Germany
    Cell 79:1221-32. 1994
    ..Since staufen is also transported with oskar (osk) mRNA during oogenesis, staufen associates specifically with both osk and bcd mRNAs to mediate their localizations, but at two distinct stages of development...
  5. ncbi Dimerization of the 3'UTR of bicoid mRNA involves a two-step mechanism
    C Wagner
    UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Descartes 67084, Strasbourg Cedex, France
    J Mol Biol 313:511-24. 2001
    ..This suggests that the stabilizing interactions that are formed during the second step of the dimerization process might represent crucial elements for Staufen recognition and localization...
  6. ncbi Localization of bicoid mRNA in late oocytes is maintained by continual active transport
    Timothy T Weil
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Dev Cell 11:251-62. 2006
    ..Furthermore, our results indicate that association of bicoid with the anterior oocyte cortex is dynamic and support a model for maintenance of bicoid localization by continual active transport on microtubules...
  7. ncbi Neuronal RNA granules: movers and makers
    Michael A Kiebler
    Center for Brain Research, Medical University of Vienna, A 1090 Vienna, Austria
    Neuron 51:685-90. 2006
    ..We focus on three classes of RNA granules that include transport RNPs, stress granules, and P bodies and discuss their potential functions in RNA localization, microRNA-mediated translational regulation, and mRNA degradation...
  8. doi Membrane targeting of Bazooka/PAR-3 is mediated by direct binding to phosphoinositide lipids
    Michael P Krahn
    Abteilung Stammzellbiologie, DFG Research Center for Molecular Physiology of the Brain CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, Gottingen, Germany
    Curr Biol 20:636-42. 2010
    ..Our findings reveal a mechanism for the coupling of plasma membrane polarity and cortical polarity...
  9. pmc Drosophila Ge-1 promotes P body formation and oskar mRNA localization
    Shih Jung Fan
    Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 6:e20612. 2011
    ..Our findings suggest an important role of dGe-1 in optimization of the osk mRNA localization process required for patterning the Drosophila embryo...
  10. pmc RNA-RNA interaction is required for the formation of specific bicoid mRNA 3' UTR-STAUFEN ribonucleoprotein particles
    D Ferrandon
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    EMBO J 16:1751-8. 1997
    ..Staufen protein (STAU) is required in a late step of the localization to anchor the bcd mRNA in the anterior cytoplasm...
  11. ncbi Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila
    Shovon I Ashraf
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 124:191-205. 2006
    ..Therefore, we propose that degradative control of the RISC pathway underlies the pattern of synaptic protein synthesis associated with a stable memory...
  12. doi Myosin-V regulates oskar mRNA localization in the Drosophila oocyte
    Jana Krauss
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, 72076 Tubingen, Germany
    Curr Biol 19:1058-63. 2009
    ..Our findings reveal that a balance of microtubule- and actin-based motor activities regulates oskar mRNA localization in the Drosophila oocyte...
  13. pmc Bazooka is required for polarisation of the Drosophila anterior-posterior axis
    Hélène Doerflinger
    The Gurdon Institute and Department of Genetics, University of Cambridge, Cambridge, UK
    Development 137:1765-73. 2010
    ..Since non-phosphorylatable Par-1 is epistatic to uninhibitable Baz, Par-1 seems to function downstream of the other PAR proteins to polarize the oocyte microtubule cytoskeleton...
  14. pmc Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufen
    A Gatignol
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    Mol Cell Biol 13:2193-202. 1993
    ..We suggest that these proteins are related by virtue of sharing a common RNA-binding moiety...
  15. ncbi Translational control of oskar generates short OSK, the isoform that induces pole plasma assembly
    F H Markussen
    Differentiation Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Development 121:3723-32. 1995
    ..Finally, we show that when oskar RNA is localized, accumulation of Oskar protein requires the functions of vasa and tudor, as well as oskar itself, suggesting a positive feedback mechanism in the induction of pole plasm by oskar...
  16. ncbi Regulated synthesis, transport and assembly of the Drosophila germ plasm
    C Rongo
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Trends Genet 12:102-9. 1996
    ..These results imply that the limiting steps in the assembly of the germ plasm are localization of the OSK RNA and regulated synthesis of the OSK protein, encoded by oskar, which are components of the germ plasm...
  17. ncbi Drosophila par-1 is required for oocyte differentiation and microtubule organization
    D N Cox
    Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California San Francisco, San Francisco, CA 94143 0725, USA
    Curr Biol 11:75-87. 2001
    ....
  18. pmc Par-1 and Tau regulate the anterior-posterior gradient of microtubules in Drosophila oocytes
    Ai Guo Tian
    Department of Biological Science, Florida State University, Tallahassee, FL 32306 4370, USA
    Dev Biol 327:458-64. 2009
    ....
  19. ncbi Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte
    D St Johnston
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, FRG
    Development 107:13-9. 1989
    ..During a final phase that must occur between stage 12 of oogenesis and egg deposition, the RNA becomes localized to a spherical region that occupies a slightly dorsal position at the anterior pole.(ABSTRACT TRUNCATED AT 250 WORDS)..
  20. ncbi Staufen: a common component of mRNA transport in oocytes and neurons?
    F Roegiers
    Howard Hughes Medical Institute, Dept of Physiology and Biochemistry, University of California, San Francisco 94143 0725, USA
    Trends Cell Biol 10:220-4. 2000
    ..The Staufen granules colocalize with ribonuclear particles that transport mRNA to the dendrites. These findings might provide clues to a mechanism of mRNA transport conserved in mammalian neurons and Drosophila oogenesis...
  21. ncbi Par-1 regulates stability of the posterior determinant Oskar by phosphorylation
    Veit Riechmann
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 10 2209, D 69012 Heidelberg, Germany
    Nat Cell Biol 4:337-42. 2002
    ..We show in cell-free extracts that Osk protein is intrinsically unstable and that it is stabilized after phosphorylation by Par-1. Our data indicate that posteriorly localized Par-1 regulates posterior patterning by stabilizing Osk...
  22. ncbi Salvador-warts-hippo signaling promotes Drosophila posterior follicle cell maturation downstream of notch
    Cedric Polesello
    Apoptosis and Proliferation Control Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2 3PX, United Kingdom
    Curr Biol 17:1864-70. 2007
    ..The PCP members of the SWH network are not involved in this process, indicating that signaling upstream of Hpo varies according to developmental context...
  23. pmc Excess protein synthesis in Drosophila fragile X mutants impairs long-term memory
    Francois V Bolduc
    Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Neurosci 11:1143-5. 2008
    ..Occlusion of long-term memory formation in Fragile X mutants could be rescued by protein synthesis inhibitors, suggesting that excess baseline protein synthesis could negatively affect cognition...
  24. ncbi The intracellular localization of messenger RNAs
    D St Johnston
    Wellcome Trust Cancer Research Campaign Institute, University of Cambridge, England
    Cell 81:161-70. 1995
    ..Indeed, there are already several examples where the direct linkage between translational control and localization has been demonstrated, and these are discussed in the accompanying review by Curtis et al. (1995)...
  25. ncbi The mago nashi locus encodes an essential product required for germ plasm assembly in Drosophila
    P A Newmark
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309
    Development 120:1303-13. 1994
    ..These results demonstrate that mago nashi encodes an essential product necessary for the localization of germ plasm components to the posterior pole of the oocyte...
  26. pmc Miranda mediates asymmetric protein and RNA localization in the developing nervous system
    A J Schuldt
    Wellcome CRC Institute and Department of Genetics, Cambridge CB2 1QR, UK
    Genes Dev 12:1847-57. 1998
    ..Thus Miranda, which localizes Prospero protein, also localizes prospero RNA through its interaction with Staufen protein...
  27. ncbi Role of Notch pathway in terminal follicle cell differentiation during Drosophila oogenesis
    M Keller Larkin
    Department of Biochemistry, J 581 Health Science Building, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Dev Genes Evol 209:301-11. 1999
    ....
  28. ncbi In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway
    B J Cha
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Cell 106:35-46. 2001
    ..We propose that microtubule-dependent Exuperantia-bicoid mRNA complex formation in the nurse cell cytoplasm allows anterior-specific transport on a grossly nonpolar oocyte microtubule network...
  29. ncbi RNA processing: splicing and the cytoplasmic localisation of mRNA
    Isabel M Palacios
    Wellcome CRC Institute, Department of Genetics, University of Cambridge, Tennis Court Road, CB2 1QR, Cambridge, UK
    Curr Biol 12:R50-2. 2002
    ..The new findings suggest that recruitment of the Mago Nashi and Y14 proteins upon splicing of oskar mRNA is an essential step in the localisation of the RNA to the posterior pole of the Drosophila oocyte...
  30. ncbi Barentsz, a new component of the Staufen-containing ribonucleoprotein particles in mammalian cells, interacts with Staufen in an RNA-dependent manner
    Paolo Macchi
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    J Neurosci 23:5778-88. 2003
    ..Together, our data suggest that the mRNA transport machinery is conserved during evolution, and that mammalian Btz is an additional component of the dendritic RNPs in hippocampal neurons...
  31. ncbi Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling
    Walter von Stein
    Abteilung Stammzellbiologie, CMPB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    Development 132:1675-86. 2005
    ..Pten mutant embryos also show severe defects during cellularization. Our data provide evidence for a link between the PAR/aPKC complex, the actin cytoskeleton and PI3-kinase signaling mediated by PTEN...
  32. ncbi Drosophila anterior-posterior polarity requires actin-dependent PAR-1 recruitment to the oocyte posterior
    Hélène Doerflinger
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Curr Biol 16:1090-5. 2006
    ..Our results therefore identify a molecular parallel between axis formation in Drosophila and C. elegans and make Drosophila PAR-1 N1 the earliest known marker for the polarization of the oocyte...
  33. pmc Dystroglycan down-regulation links EGF receptor signaling and anterior-posterior polarity formation in the Drosophila oocyte
    John S Poulton
    Department of Biological Science, Florida State University, Tallahassee, FL 32306 4370, USA
    Proc Natl Acad Sci U S A 103:12775-80. 2006
    ..Our data indicate that Dystroglycan links EGFR-induced repression of the anterior follicle cell fate and anterior-posterior polarity formation in the oocyte...
  34. pmc Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte
    Cuiyun Geng
    Institute for Cellular and Molecular Biology, Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712 0159, USA
    Mol Cell Biol 26:9508-16. 2006
    ..The opposing effects of reduced and elevated Imp activity on gurken mRNA expression indicate a role in gurken mRNA regulation...
  35. pmc Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary
    Sarah Mische
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 18:2254-63. 2007
    ..We speculate that after delivery to the oocyte, RNP complexes may disassemble and be remodeled with appropriate accessory factors to ensure proper localization...
  36. doi Lethal(2)giant larvae is required in the follicle cells for formation of the initial AP asymmetry and the oocyte polarity during Drosophila oogenesis
    Qi Li
    Laboratory of Developmental Genetics, Bio X Life Science Research Center and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
    Cell Res 18:372-84. 2008
    ..Thus, we provide the first demonstration that lgl is implicated in the formation of the initial AP asymmetry and the patterning of the AP and DV axes in the oocyte by acting in the specification of a subset of somatic follicle cells...
  37. pmc The hippo pathway promotes Notch signaling in regulation of cell differentiation, proliferation, and oocyte polarity
    Jianzhong Yu
    Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America
    PLoS ONE 3:e1761. 2008
    ....
  38. pmc A conserved double-stranded RNA-binding domain
    D St Johnston
    Wellcome Cancer Research Campaign Institute, Cambridge, United Kingdom
    Proc Natl Acad Sci U S A 89:10979-83. 1992
    ..On the basis of the binding studies and computer analysis, we have derived a consensus sequence that defines a 65- to 68-amino acid dsRNA-binding domain...
  39. ncbi Staufen, a gene required to localize maternal RNAs in the Drosophila egg
    D St Johnston
    Max Planck Institut für Entwicklungsbiologie Abteilung III Genetik, Tubingen, Federal Republic of Germany
    Cell 66:51-63. 1991
    ..By the time the egg is laid, staufen protein is also concentrated at the anterior pole, in the same region as bicoid RNA...
  40. ncbi oskar mRNA is localized to the posterior pole of the Drosophila oocyte
    J Kim-Ha
    Department of Biological Science, Stanford University, California 94305 5020
    Cell 66:23-35. 1991
    ..In addition, we find that nonsense oskar mutations disrupt osk mRNA localization, while missense oskar mutations do not...
  41. ncbi Oskar organizes the germ plasm and directs localization of the posterior determinant nanos
    A Ephrussi
    Whitehead Institute for Biomedical Research Howard Hughes Medical Institute, Cambridge, Massachusetts 02142
    Cell 66:37-50. 1991
    ..We propose that the pole plasm is assembled stepwise and that continued interaction among its components is required for germ cell determination...
  42. ncbi Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development
    P F Lasko
    Department of Genetics, University of Cambridge, UK
    Genes Dev 4:905-21. 1990
    ..These results are discussed with respect to the multiple functions of the vasa gene...
  43. ncbi Germline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila
    T Schupbach
    Dev Biol 113:443-8. 1986
    ....
  44. ncbi Localization of oskar RNA regulates oskar translation and requires Oskar protein
    C Rongo
    Whitehead Institute for Biomedical Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02142, USA
    Development 121:2737-46. 1995
    ..We propose that initially localization of oskar RNA permits translation into Oskar protein and that subsequently Oskar protein regulates its own RNA localization through a positive feedback mechanism...
  45. ncbi Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization
    M Erdelyi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nature 377:524-7. 1995
    ..Here we show that mutations in tropomyosin II (TmII) virtually abolish oskar RNA localization to the posterior pole, suggesting an involvement of the actin network in oskar RNA localization...
  46. ncbi Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential
    J Kim-Ha
    Department of Biological Sciences, Stanford University, California 94305 5020, USA
    Cell 81:403-12. 1995
    ..Addition of BREs to a heterologous mRNA renders it sensitive to translational repression in the ovary...
  47. ncbi Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte
    I Clark
    Howard Hughes Medical Institute, University of California at San Francisco 94143 0724
    Curr Biol 4:289-300. 1994
    ..The mechanism by which these two early components accumulate at the posterior is not known. We have investigated whether directed transport along microtubules could be used to accomplish this localization...
  48. ncbi Role of oocyte position in establishment of anterior-posterior polarity in Drosophila
    A González-Reyes
    Wellcome CRC Institute, University of Cambridge, England
    Science 266:639-42. 1994
    ..Moreover, the generation of AP asymmetry requires signaling from the germ line to the soma and back again...
  49. ncbi Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities
    L Liang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Development 120:1201-11. 1994
    ..Posterior localization of vasa protein depends upon the functions of four genes: capu, spir, osk and stau. We have found that localization of vasa to the perinuclear nuage is abolished in most vas alleles, but is ..
  50. ncbi Multiple RNA regulatory elements mediate distinct steps in localization of oskar mRNA
    J Kim-Ha
    Department of Biological Sciences, Stanford University, CA 94305
    Development 119:169-78. 1993
    ....
  51. ncbi Profilin is required for posterior patterning of the Drosophila oocyte
    L Manseau
    Department of Molecular and Cellular Biology, Universiity of Arizona, Tucson, AZ 85721, USA
    Development 122:2109-16. 1996
    ..This, together with the similarity of mutant phenotypes, suggests that profilin and CAPPUCCINO may interact during development...
  52. ncbi Oskar protein interaction with Vasa represents an essential step in polar granule assembly
    W Breitwieser
    Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Genes Dev 10:2179-88. 1996
    ..These results suggest that the Oskar-Vasa interaction constitutes an initial step in polar granule assembly. In addition, we discuss the possible biological role of the Oskar-Staufen interaction...
  53. ncbi Cytoplasmic flows localize injected oskar RNA in Drosophila oocytes
    J B Glotzer
    Programme in Developmental Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, D 69117, Germany
    Curr Biol 7:326-37. 1997
    ..The mechanisms by which osk RNA is localized are unknown...
  54. ncbi Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions
    P Li
    Developmental Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore
    Cell 90:437-47. 1997
    ..b>Stau, as one component of this complex, is required only for RNA localization...
  55. ncbi Staufen-dependent localization of prospero mRNA contributes to neuroblast daughter-cell fate
    J Broadus
    Department of Cell and Structural Biology, Howard Hughes Medical Institute, University of Illinois, Urbana 61801, USA
    Nature 391:792-5. 1998
    ..The prospero (pros) mRNA and the RNA-binding protein Staufen (Stau) are asymmetrically localized in mitotic neuroblasts and are specifically partitioned into the GMC, as is Pros ..
  56. pmc Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila
    C P Shen
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0725 USA
    Genes Dev 12:1837-46. 1998
    ..Our results suggest that Miranda directs the basal cortical localization of multiple molecules, including Staufen and prospero RNA, in mitotic neuroblasts in an actin-dependent manner...
  57. ncbi RNA sorting in Drosophila oocytes and embryos
    P Lasko
    Departments of Biology and Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 1B1
    FASEB J 13:421-33. 1999
    ..Prospects for filling gaps in our knowledge about the mechanisms of localizing RNAs and the importance of RNA sorting in regulating gene expression are also explored...
  58. ncbi Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
    A Wodarz
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, Germany
    Nature 402:544-7. 1999
    ..As Bazooka is also responsible for the maintenance of apical-basal polarity in epithelial tissues, it may be the missing link between epithelial polarity and neuroblast polarity...
  59. pmc RNA recognition by a Staufen double-stranded RNA-binding domain
    A Ramos
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH
    EMBO J 19:997-1009. 2000
    ..In addition, helix alpha1 interacts with the single-stranded loop that caps the RNA helix. Interactions between helix alpha1 and single-stranded RNA may be important determinants of the specificity of dsRBD proteins...
  60. pmc Distinct roles of two conserved Staufen domains in oskar mRNA localization and translation
    D R Micklem
    Wellcome CRC Institute, Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 19:1366-77. 2000
    ..Since dsRBD5 has been shown to direct the actin-dependent localization of prospero mRNA, distinct domains of Staufen mediate microtubule- and actin-based mRNA transport...
  61. ncbi The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole
    J M Shulman
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, United Kingdom
    Cell 101:377-88. 2000
    ..These results identify a molecular parallel between anterior-posterior polarization in Drosophila and C. elegans...
  62. ncbi A Drosophila melanogaster homologue of Caenorhabditis elegans par-1 acts at an early step in embryonic-axis formation
    P Tomancak
    Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Cell Biol 2:458-60. 2000
  63. pmc A function for kinesin I in the posterior transport of oskar mRNA and Staufen protein
    R P Brendza
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Science 289:2120-2. 2000
    ..Thus, a complex containing oskar mRNA and Staufen may be transported along microtubules to the posterior pole by kinesin I...
  64. pmc Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis
    H Lopez-Schier
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Cambridge CB2 1QR, UK
    Genes Dev 15:1393-405. 2001
    ..The germ line therefore regulates the development of the follicle cells through two complementary signaling pathways: Gurken signals twice to control spatial patterning, whereas Delta signals twice to exert temporal control...
  65. ncbi Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila
    J R Huynh
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, CB21QR, Cambridge, United Kingdom
    Curr Biol 11:901-6. 2001
    ..elegans and Drosophila, the relationships between them are different, as the localization of PAR-1 does not require Bazooka or PAR-6 in Drosophila, as it does in the worm...
  66. ncbi Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport
    O Hachet
    Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 11:1666-74. 2001
    ..It was recently shown that human Y14, a nuclear protein that associates with mRNAs upon splicing and shuttles to the cytoplasm, interacts with MAGOH, the human homolog of Mago-nashi...
  67. ncbi Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis
    S L Bullock
    Developmental Genetics Laboratory, Imperial Cancer Research Fund, London, UK
    Nature 414:611-6. 2001
    ..We propose that Egl and BicD are core components of a selective dynein motor complex that drives transcript localization in a variety of tissues...
  68. ncbi Oskar anchoring restricts pole plasm formation to the posterior of the Drosophila oocyte
    Nathalie F Vanzo
    Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1 69117 Heidelberg, Germany
    Development 129:3705-14. 2002
    ..This study reveals cortical anchoring of the posterior determinant Oskar as a crucial step in pole plasm assembly and restriction, required for proper development of Drosophila melanogaster...
  69. ncbi Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte
    Isabel M Palacios
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, UK
    Development 129:5473-85. 2002
    ..Thus, the Kinesin heavy chain can function independently of the light chain in the oocyte, indicating that it associates with its cargoes by a novel mechanism...
  70. ncbi Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation
    Richard Benton
    The Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, CB2 1QR, Cambridge, United Kingdom
    Dev Cell 3:659-71. 2002
    ..The C. elegans 14-3-3 protein, PAR-5, is also required for A-P polarization, suggesting that this is a conserved mechanism by which PAR-1 establishes cellular asymmetries...
  71. ncbi The nuclear connection in RNA transport and localization
    Kim L Farina
    Dept of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Trends Cell Biol 12:466-72. 2002
    ..These proteins might link nuclear events, such as RNA splicing, with the subsequent cytoplasmic localization of specific transcripts...
  72. ncbi Orb and a long poly(A) tail are required for efficient oskar translation at the posterior pole of the Drosophila oocyte
    Stefania Castagnetti
    Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 130:835-43. 2003
    ..We conclude that Orb-mediated cytoplasmic polyadenylation stimulates oskar translation to achieve the high levels of Oskar protein necessary for posterior patterning and germline differentiation...
  73. ncbi A Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila
    Isabel L Torres
    The Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Dev Cell 5:547-58. 2003
    ..The anterior-posterior axis is therefore established by a relay mechanism, which propagates polarity from one cyst to the next...
  74. ncbi Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells
    Richard Benton
    The Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, CB2 1QR Cambridge, United Kingdom
    Cell 115:691-704. 2003
    ..Thus, antagonism of Bazooka by PAR-1/14-3-3 may represent a general mechanism for establishing complementary cortical domains in polarized cells...
  75. ncbi The Drosophila hnRNPA/B homolog, Hrp48, is specifically required for a distinct step in osk mRNA localization
    Jean René Huynh
    The Wellcome Trust Cancer Research UK, Gurdon Institute and Department of Genetics, Cambridge University, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Dev Cell 6:625-35. 2004
    ..This suggests a new step in the localization pathway, which may correspond to the assembly of Staufen/oskar mRNA transport particles...
  76. ncbi Hrp48, a Drosophila hnRNPA/B homolog, binds and regulates translation of oskar mRNA
    Tamaki Yano
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Dev Cell 6:637-48. 2004
    ..Our data also show that Hrp48, which binds to the 5' and 3' regions of oskar mRNA, plays an important role in restricting Oskar activity to the posterior of the oocyte, by repressing oskar mRNA translation during transport...
  77. ncbi Localization-dependent oskar protein accumulation; control after the initiation of translation
    Arie Koen Braat
    Institute for Cellular and Molecular Biology, Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712, USA
    Dev Cell 7:125-31. 2004
    ..Thus, the mechanisms that prevent accumulation of Oskar protein until it can be secured at the posterior pole of the oocyte include regulated degradation or inhibition of translational elongation...
  78. pmc A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP
    Trent P Munro
    The Gurdon Institute, University of Cambridge, Cambridge CB2 1QR, England, UK
    J Cell Biol 172:577-88. 2006
    ..Our findings establish a parallel requirement for IBEs in the regulation of localized maternal mRNAs in D. melanogaster and X. laevis...
  79. ncbi Drosophila decapping protein 1, dDcp1, is a component of the oskar mRNP complex and directs its posterior localization in the oocyte
    Ming Der Lin
    Institute of Molecular and Cellular Biology, College of Life Science, National Taiwan University, Number 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan
    Dev Cell 10:601-13. 2006
    ..Thus, as well as being a general factor required for mRNA decay, dDcp1 is an essential component of the osk mRNP localization complex...
  80. ncbi A translation-independent role of oskar RNA in early Drosophila oogenesis
    Andreas Jenny
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 133:2827-33. 2006
    ..Our analysis thus reveals an unexpected role for oskar RNA during early oogenesis, independent of Oskar protein. These findings indicate that oskar RNA acts as a scaffold or regulatory RNA essential for development of the oocyte...
  81. pmc Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies
    Scott A Barbee
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    Neuron 52:997-1009. 2006
    ..These results argue for a conserved mechanism of translational control critical to neuronal function and open up new experimental avenues for understanding the regulation of mRNA function within neurons...
  82. pmc bicoid RNA localization requires specific binding of an endosomal sorting complex
    Uwe Irion
    Wellcome Trust Cancer Research UK Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 445:554-8. 2007
    ..This function of ESCRT-II as an RNA-binding complex is conserved in vertebrates and may clarify some of its roles that are independent of endosomal sorting...
  83. pmc An oskar-dependent positive feedback loop maintains the polarity of the Drosophila oocyte
    Vitaly Zimyanin
    Max Planck Institute of Molecular Cell Biology and Genetics, 108 Pfotenhauerstrasse, 01307 Dresden, Germany
    Curr Biol 17:353-9. 2007
    ....
  84. ncbi Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis
    Jean Baptiste Coutelis
    Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Development 134:1419-30. 2007
    ..These findings highlight the central role of vesicular trafficking in the establishment of polarity and in determinant localization in Drosophila...
  85. ncbi Rab6 and the secretory pathway affect oocyte polarity in Drosophila
    Jens Januschke
    Institut Jacques Monod, Unite Mixte de Recherche 7592, CNRS, Universités Paris 6 et Paris 7, 2 place Jussieu, F 75251, Paris Cedex 05, France
    Development 134:3419-25. 2007
    ..Our results point to a possible connection between Rab protein-mediated secretion, organization of the cytoskeleton and mRNA transport...
  86. pmc The salvador-warts-hippo pathway is required for epithelial proliferation and axis specification in Drosophila
    Carine Meignin
    Wellcome Trust Centre for Cell Biology, Kings Buildings, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Curr Biol 17:1871-8. 2007
    ..This work highlights a novel connection between cell proliferation, cell growth, and axis specification in egg chambers...
  87. doi The endocytic pathway acts downstream of Oskar in Drosophila germ plasm assembly
    Tsubasa Tanaka
    Laboratory for Germline Development, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Development 135:1107-17. 2008
    ..We propose that Osk stimulates endosomal cycling, which in turn promotes F-actin reorganization to anchor the pole plasm components to the oocyte cortex...
  88. doi Drosophila processing bodies in oogenesis
    Ming Der Lin
    Institute of Molecular and Cellular Biology, College of Life Science, National Taiwan University, Taipei, Taiwan
    Dev Biol 322:276-88. 2008
    ..This also suggests that a regulated conversion occurs between maternal RNA granules and P-bodies from oogenesis to embryogenesis...
  89. pmc In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization
    Vitaly L Zimyanin
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, CB2 1QN Cambridge, UK
    Cell 134:843-53. 2008
    ..We also show that each component of the oskar mRNA complex plays a distinct role in particle formation and transport...
  90. doi Drosophila ensconsin promotes productive recruitment of Kinesin-1 to microtubules
    Hsin Ho Sung
    Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore
    Dev Cell 15:866-76. 2008
    ..Furthermore, spatial control of motor recruitment can provide additional regulatory control in Par-1 and microtubule-dependent cell polarity...
  91. pmc Dynamic organization and plasticity of sponge bodies
    Mark J Snee
    Institute for Cellular and Molecular Biology, Section of Molecular, Cell, and Developmental Biology, The University of Texas at Austin, Austin, Texas, USA
    Dev Dyn 238:918-30. 2009
    ..Based on these and other results we propose a model for the relationship between P bodies and the various cytoplasmic bodies containing P body proteins in the Drosophila ovary...
  92. pmc Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility
    Philippe Loiseau
    The Zoology Department, University of Cambridge, Cambridge, UK
    Development 137:2763-72. 2010
    ..This work suggests that the role of PAT1 in cargo transport in the cell is linked to PAT1 function as a positive regulator of Kinesin motility...
  93. pmc A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte
    Richard M Parton
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England, UK
    J Cell Biol 194:121-35. 2011
    ..Our findings explain the biased random posterior cargo movements in the oocyte that establish the germline and posterior...
  94. pmc Dimerization of oskar 3' UTRs promotes hitchhiking for RNA localization in the Drosophila oocyte
    Helena Jambor
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 17:2049-57. 2011
    ..Our analysis provides insight into the molecular basis of RNA hitchhiking, whereby localization-incompetent RNA molecules can become locally enriched in the cytoplasm, by virtue of their association with transport-competent RNAs...
  95. doi A Cdc42-regulated actin cytoskeleton mediates Drosophila oocyte polarization
    Andrea Leibfried
    Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Development 140:362-71. 2013
    ..This most likely allows for the robustness in symmetry breaking in the cell...
  96. pmc Oskar is targeted for degradation by the sequential action of Par-1, GSK-3, and the SCF⁻Slimb ubiquitin ligase
    Eurico Morais-de-Sá
    The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Dev Cell 26:303-14. 2013
    ..These results reveal that Par-1 controls the timing of pole plasm assembly by promoting the localization of oskar mRNA but inhibiting the accumulation of Short Oskar protein...
  97. ncbi Induction of germ cell formation by oskar
    A Ephrussi
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Nature 358:387-92. 1992
    ..Of the eight genes necessary for germ cell formation at the posterior, only three, oskar, vasa and tudor, are essential at an ectopic site...
  98. ncbi A gradient of JAK pathway activity patterns the anterior-posterior axis of the follicular epithelium
    Rongwen Xi
    University of Kentucky, Department of Biology, 101 Morgan Building, Lexington, KY 40506, USA
    Dev Cell 4:167-77. 2003
    ..We propose that Upd secreted from the polar cells may act as a morphogen to stimulate A/P-derived follicular fates through JAK pathway activation...
  99. ncbi Role of gap genes in early Drosophila development
    U Gaul
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Federal Republic of Germany
    Adv Genet 27:239-75. 1990
  100. ncbi Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos
    J W Raff
    Department of Biochemistry, The University of Dundee, Scotland
    Development 110:1249-61. 1990
    ..The distribution pattern of the transcript at the posterior pole throughout embryogenesis and in a variety of mutant embryos suggests that this component is associated with polar granules...
  101. pmc Female sterile mutations on the second chromosome of Drosophila melanogaster. I. Maternal effect mutations
    T Schupbach
    Biology Department, Princeton University, New Jersey 08544
    Genetics 121:101-17. 1989
    ....

Research Grants5

  1. GENETIC ANALYSIS OF PREHAIR MORPHOGENESIS
    Paul Adler; Fiscal Year: 2005
    ....
  2. ASYMMETRIC CELL DIVISION IN C. ELEGANS EMBRYOS
    Kenneth Kemphues; Fiscal Year: 2005
    ....
  3. NEUROLOGICAL FUNCTION OF FRAGILE X GENE IN DROSOPHILA
    Kendal Broadie; Fiscal Year: 2006
    ..Specific Aim 3 will search for novel dFXR interacting genes with a genetic enhancer / suppressor screening and microarray technology. This application therefore complements and extends the parallel studies on mammalian systems. ..
  4. Synaptic Mechanisms in Drosophila Neurodegeneration
    Kendal Broadie; Fiscal Year: 2009
    ..abstract_text> ..
  5. GENETIC ANALYSIS OF SYNAPSE FORMATION AND FUNCTION
    Kendal Broadie; Fiscal Year: 2010
    ..These aims will include confocal and electron microscopy imaging, synaptic electrophysiological recording and extensive molecular genetic studies. We will continue screens for synaptogenesis mutants. ..