AGO2

Summary

Gene Symbol: AGO2
Description: argonaute 2, RISC catalytic component
Alias: CASC7, EIF2C2, LINC00980, PPD, Q10, protein argonaute-2, PAZ Piwi domain protein, argonaute RISC catalytic component 2, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 980, protein slicer
Species: human

Top Publications

  1. ncbi Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004
  2. doi Argonaute proteins couple chromatin silencing to alternative splicing
    Maya Ameyar-Zazoua
    Universite Paris Sud, Laboratoire Epigénétique et Cancer, Formation de Recherche en Evolution, Gif sur Yvette, France
    Nat Struct Mol Biol 19:998-1004. 2012
  3. pmc The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference
    Madeeha Aqil
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
    PLoS ONE 8:e74472. 2013
  4. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
  5. doi Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2
    Filipp Frank
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 0B1, Canada
    Nature 465:818-22. 2010
  6. pmc HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies
    Prabhjeet K Phalora
    Department of Infectious Diseases, Kings College London School of Medicine, Guy s Hospital, London, United Kingdom
    J Virol 86:11712-24. 2012
  7. pmc Hypomethylation of intragenic LINE-1 represses transcription in cancer cells through AGO2
    Chatchawit Aporntewan
    Department of Mathematics, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
    PLoS ONE 6:e17934. 2011
  8. pmc HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly
    Jonathan C Reed
    Department of Global Health, University of Washington, Seattle, WA 98102, USA
    J Cell Biol 198:439-56. 2012
  9. pmc Phosphorylation of human Argonaute proteins affects small RNA binding
    Sabine Rüdel
    Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nucleic Acids Res 39:2330-43. 2011
  10. doi Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer
    Michael Sand
    Department of Dermatology, Venereology and Allergology, Ruhr University Bochum, Bochum, Germany
    Mol Carcinog 51:916-22. 2012

Research Grants

  1. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2012
  2. CoEnzyme Q10 in Statin Myopathy
    PAUL DAVIS THOMPSON; Fiscal Year: 2010
  3. Catarina M Quinzii; Fiscal Year: 2014
  4. Biomarkers of Oxidative Stress in Parkinson's Disease
    M Flint Beal; Fiscal Year: 2010
  5. Jack D Keene; Fiscal Year: 2015
  6. Anti-oxidant treatment of muscle wasting and fatigue in tumor-bearing mice
    YVONNE YUMIKO CLARK; Fiscal Year: 2012
  7. FREE RADICAL LIPID PEROXIDATION DAMAGE
    ALOYS TAPPEL; Fiscal Year: 1991
  8. PREQUEL Study In PRE-manifest HD of CoQ10/UbiquinonE Leading to Preventive Trials
    Christopher A Ross; Fiscal Year: 2010
  9. Effects of Antioxidant Supplementation during Breast Cancer Adjuvant Therapy
    Heather Greenlee; Fiscal Year: 2013
  10. Coenzyme Q10 in Huntingtons Disease
    Merit E Cudkowicz; Fiscal Year: 2013

Detail Information

Publications231 found, 100 shown here

  1. ncbi Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004
    ..Thus, our evidence supports a model in which Argonaute contributes "Slicer" activity to RISC, providing the catalytic engine for RNAi...
  2. doi Argonaute proteins couple chromatin silencing to alternative splicing
    Maya Ameyar-Zazoua
    Universite Paris Sud, Laboratoire Epigénétique et Cancer, Formation de Recherche en Evolution, Gif sur Yvette, France
    Nat Struct Mol Biol 19:998-1004. 2012
    ..Here, we have immunopurified human Argonaute-1 and Argonaute-2 (AGO1 and AGO2) chromatin-embedded proteins and found them associated with chromatin modifiers and, notably, with splicing factors...
  3. pmc The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference
    Madeeha Aqil
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
    PLoS ONE 8:e74472. 2013
    ..The RNA interference (RNAi) pathway proteins Ago2 and GW182 localize to MVBs, suggesting these to be sites for assembly and turnover of the miRNA-induced silencing ..
  4. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
    ....
  5. doi Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2
    Filipp Frank
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 0B1, Canada
    Nature 465:818-22. 2010
    ..Here we report the crystal structure of a MID domain from a eukaryotic AGO protein, human AGO2. The structure, in complex with nucleoside monophosphates (AMP, CMP, GMP, and UMP) mimicking the 5' end of miRNAs, ..
  6. pmc HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies
    Prabhjeet K Phalora
    Department of Infectious Diseases, Kings College London School of Medicine, Guy s Hospital, London, United Kingdom
    J Virol 86:11712-24. 2012
    ..Localization to P bodies may therefore provide a means of sequestering APOBEC3 enzymatic activity away from cellular DNA or may be linked to as yet unidentified cellular functions...
  7. pmc Hypomethylation of intragenic LINE-1 represses transcription in cancer cells through AGO2
    Chatchawit Aporntewan
    Department of Mathematics, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
    PLoS ONE 6:e17934. 2011
    ..In contrast, many mRNAs derived from genes containing L1s are elevated in Argonaute 2 (AGO2 or EIF2C2)-depleted cells. Hypomethylated L1s increase L1 mRNA levels...
  8. pmc HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly
    Jonathan C Reed
    Department of Global Health, University of Washington, Seattle, WA 98102, USA
    J Cell Biol 198:439-56. 2012
    ..IEM) that these Gag-containing AIs also contain endogenous processing body (PB)-related proteins, including AGO2 and the ribonucleic acid (RNA) helicase DDX6...
  9. pmc Phosphorylation of human Argonaute proteins affects small RNA binding
    Sabine Rüdel
    Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nucleic Acids Res 39:2330-43. 2011
    ..Here we report a comprehensive analysis of Ago2 phosphorylation in human cells...
  10. doi Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer
    Michael Sand
    Department of Dermatology, Venereology and Allergology, Ruhr University Bochum, Bochum, Germany
    Mol Carcinog 51:916-22. 2012
    ..subunit DiGeorge syndrome critical region gene 8 (DGCR8) and the RISC components argonaute-1 (AGO1), argonaute-2 (AGO2), as well as double-stranded RNA-binding proteins PACT, TARBP1, and TARBP2 in epithelial skin cancer and its ..
  11. ncbi Identification of eight members of the Argonaute family in the human genome
    Takashi Sasaki
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo, 160 8582, Japan
    Genomics 82:323-30. 2003
    ..the PIWI subfamily (PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and PIWIL4/HIWI2) and the eIF2C/AGO subfamily (EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and EIF2C4/hAGO4)...
  12. ncbi An mRNA m7G cap binding-like motif within human Ago2 represses translation
    Marianthi Kiriakidou
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Cell 129:1141-51. 2007
    ..We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of ..
  13. pmc Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2
    Aarti Jagannath
    Department of Physiology, Anatomy, and Genetics, University of Oxford, United Kingdom
    Mol Biol Cell 20:521-9. 2009
    ..Key effectors of microRNA (miRNA)-mediated RNA interference (RNAi), such as Argonaute-2 (Ago2), miRNAs, and their cognate mRNAs, are localized to these structures; however, the precise role that P-bodies and ..
  14. pmc Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma
    Yohei Horikawa
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 14:7956-62. 2008
    ..Compelling evidence has shown that miRNAs are involved in cancer initiation and progression. We hypothesized that genetic variations of the miRNA machinery genes could be associated with the risk of renal cell carcinoma...
  15. doi Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins
    Bingbing Wang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Struct Mol Biol 16:1259-66. 2009
    ..Using a minimal in vitro system, we show that bacterially expressed human AGO1 and AGO2 but not AGO3 and AGO4 possess strand-dissociating activity of microRNA (miRNA) duplexes...
  16. pmc Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies
    Michael J Wichroski
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Pathog 2:e41. 2006
    ..and eIF4E-T), translation suppression (RCK/p54), RNA interference-mediated post-transcriptional gene silencing (AGO2), and decapping of mRNA (DCP2)...
  17. ncbi Single-stranded antisense siRNAs guide target RNA cleavage in RNAi
    Javier Martinez
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077, Gottingen, Germany
    Cell 110:563-74. 2002
    ..that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with eIF2C1 and/or eIF2C2 (human GERp95) Argonaute proteins...
  18. pmc ATP-dependent human RISC assembly pathways
    Mayuko Yoda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Nat Struct Mol Biol 17:17-23. 2010
    ..All these features of human AGO proteins are highly reminiscent of fly Ago1 but not fly Ago2.
  19. pmc Argonaute proteins: potential biomarkers for human colon cancer
    Lan Li
    Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China
    BMC Cancer 10:38. 2010
    ..In this study, we investigated the expression of eight members of human Argonaute family in colon cancer and identified their potential roles in tumor development and progression...
  20. pmc Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation
    Joyce A Wilson
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada
    J Virol 85:2342-50. 2011
    ..Argonaute 2 (Ago2) is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed ..
  21. pmc Stabilization of hepatitis C virus RNA by an Ago2-miR-122 complex
    Tetsuro Shimakami
    Lineberger Comprehensive Cancer Center and Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7292, USA
    Proc Natl Acad Sci U S A 109:941-6. 2012
    ..Here we show that miR-122 binds HCV RNA in association with Ago2 and that this slows decay of the viral genome in infected cells...
  22. pmc The structure of human argonaute-2 in complex with miR-20a
    Elad Elkayam
    W M Keck Structural Biology Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 150:100-10. 2012
    ..Here, we report the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 Å resolution...
  23. pmc Highly complementary target RNAs promote release of guide RNAs from human Argonaute2
    Nabanita De
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92121, USA
    Mol Cell 50:344-55. 2013
    ..Here we show that the Argonaute2 (Ago2) guide RNA complex is extremely stable, with a half-life on the order of days...
  24. ncbi Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression
    R Koesters
    Institute of Clinical Pathology, Department of Pathology, University Hospital of Zurich, Schmelzbergstrasse 12, Zurich, 8091, Switzerland
    Genomics 61:210-8. 1999
    ..It is highly conserved during evolution, sharing about 90% identity with rabbit eIF2C and 70% identity with plant AGO1 at the amino acid level. These facts suggest that human EIF2C1 might play an important physiological role...
  25. ncbi The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 16:2733-42. 2002
  26. ncbi Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors
    Noboru Doi
    Department of Biophysics, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, 113 0033, Bunkyo ku, Tokyo, Japan
    Curr Biol 13:41-6. 2003
    ..Immunoprecipitation experiments suggest that, in human and mouse cells, complex formation occurs between Dicer and eIF2C1 or 2 and that the PIWI domain of eIF2C is essential for the formation of this complex...
  27. pmc Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer
    Nasser Tahbaz
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    EMBO Rep 5:189-94. 2004
    PAZ PIWI domain (PPD) proteins, together with the RNA cleavage products of Dicer, form ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs)...
  28. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..complexes from different human cell lines revealed that endonuclease activity is exclusively associated with Ago2. Exogenously introduced siRNAs also associate with Ago2 for guiding target RNA cleavage...
  29. ncbi Involvement of microRNA in AU-rich element-mediated mRNA instability
    Qing Jing
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 120:623-34. 2005
    ..An RNAi-based screen performed in Drosophila S2 cells has revealed that Dicer1, Argonaute1 (Ago1) and Ago2, components involved in microRNA (miRNA) processing and function, are required for the rapid decay of mRNA ..
  30. ncbi Purified Argonaute2 and an siRNA form recombinant human RISC
    Fabiola V Rivas
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, New York 11724, USA
    Nat Struct Mol Biol 12:340-9. 2005
    ..These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex...
  31. ncbi Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies
    George L Sen
    Department of Molecular Pharmacology, 269 Campus Drive, CCSR 4225A, Stanford University School of Medicine, Stanford, CA 94305, USA
    Nat Cell Biol 7:633-6. 2005
    ..In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function...
  32. pmc MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nat Cell Biol 7:719-23. 2005
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  33. pmc TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 436:740-4. 2005
    ..Biochemical analysis of TRBP-containing complexes revealed the association of Dicer-TRBP with Argonaute 2 (Ago2), the catalytic engine of RISC...
  34. ncbi Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes
    Christian Matranga
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell 123:607-20. 2005
    In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is the catalytic component of the RNAi enzyme complex, RISC...
  35. pmc Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity
    Bryan A Kraynack
    Isis Pharmaceuticals, Carlsbad Research Center, 1896 Rutherford Road, Carlsbad, CA 92008, USA
    RNA 12:163-76. 2006
    ..We further show, by using biotin-tagged siRNAs and affinity-tagged hAgo2/eIF2C2, that activity of siRNA duplexes containing full 2'-OMe substitutions in the sense strand is mediated by the RNA-..
  36. pmc A human, ATP-independent, RISC assembly machine fueled by pre-miRNA
    Elisavet Maniataki
    Department of Pathology, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Genes Dev 19:2979-90. 2005
    ..In humans, the catalytic engine of RISC is a ribonucleoprotein formed by the Argonaute2 (Ago2) protein and either miRNA (miRNP) or siRNA (siRNP)...
  37. pmc The role of PACT in the RNA silencing pathway
    Yoontae Lee
    Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul, Korea
    EMBO J 25:522-32. 2006
    ..Our study indicates that, unlike other RNase III type proteins, human Dicer may employ two different dsRBD-containing proteins that facilitate RISC assembly...
  38. pmc Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54
    Chia Ying Chu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 4:e210. 2006
    ..Both RISCs contain Argonaute2 (Ago2), which catalyzes target mRNA cleavage by siRISC and localizes to cytoplasmic mRNA processing bodies (P-bodies)...
  39. ncbi Involvement of AGO1 and AGO2 in mammalian transcriptional silencing
    Bethany A Janowski
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Nat Struct Mol Biol 13:787-92. 2006
    ..Here we show that the human homologs of Argonaute-1 (AGO1) and Argonaute-2 (AGO2) link the silencing pathways that target mRNA with pathways mediating recognition of DNA...
  40. pmc Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules
    Anthony K L Leung
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:18125-30. 2006
    ..Further, miRNAs are required for the Argonaute protein localization to SGs but not PBs. These quantitative kinetic data provide insights into miRNA-mediated repression...
  41. ncbi MicroRNA silencing through RISC recruitment of eIF6
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 447:823-8. 2007
    ..These results uncover an evolutionarily conserved function of the ribosome anti-association factor eIF6 in miRNA-mediated post-transcriptional silencing...
  42. pmc Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system
    Motoaki Wakiyama
    RIKEN Genomic Sciences Center, Protein Research Group, Tsurumi, Yokohama 230 0045, Japan
    Genes Dev 21:1857-62. 2007
    ..Our work suggests that let-7 miRNPs containing Argonaute and GW182 impair the synergistic enhancement of translation by the 5'-cap and 3'-poly(A) tail, resulting in translational repression...
  43. ncbi A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain
    Susanne Till
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Struct Mol Biol 14:897-903. 2007
    ..Proteins containing the conserved Ago hook may thus be important regulatory components of effector complexes in RNA interference...
  44. pmc In vitro reconstitution of the human RISC-loading complex
    Ian J Macrae
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:512-7. 2008
    ..In humans, Ago2 is loaded with miRNAs by the action of a specialized assembly called the RISC-loading complex (RLC), comprising the ..
  45. doi Disappearance of the angiogenic potential of endothelial cells caused by Argonaute2 knockdown
    Tomohiro Asai
    Department of Medical Biochemistry and Global COE, University of Shizuoka School of Pharmaceutical Sciences, 52 1 Yada, Suruga ku, Shizuoka 422 8526, Japan
    Biochem Biophys Res Commun 368:243-8. 2008
    Argonaute2 (Ago2), a component protein of RNA-induced silencing complex, plays a central role in RNA interference. We focused on the involvement of Ago2 in angiogenesis...
  46. doi Phosphorylation of Argonaute 2 at serine-387 facilitates its localization to processing bodies
    Yan Zeng
    Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA
    Biochem J 413:429-36. 2008
    ..To explore potential regulatory mechanisms for Ago proteins, we examined the phosphorylation of human Ago2. We identified serine-387 as the major Ago2 phosphorylation site in vivo...
  47. pmc Huntington's disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies
    Jeffrey N Savas
    Department of Microbiology and New York University Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 105:10820-5. 2008
    ..Colocalization studies demonstrated Htt and Ago2 to be present in P bodies, and depletion of Htt showed compromised RNA-mediated gene silencing...
  48. pmc Prolyl 4-hydroxylation regulates Argonaute 2 stability
    Hank H Qi
    Department of Pathology, Harvard Medical School, New Research Building 854, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 455:421-4. 2008
    Human Argonaute (Ago) proteins are essential components of the RNA-induced silencing complexes (RISCs). Argonaute 2 (Ago2) has a P-element-induced wimpy testis (PIWI) domain, which folds like RNase H and is responsible for target RNA ..
  49. pmc Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells
    Brian D Adams
    Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3505, USA
    Endocrinology 150:14-23. 2009
    ..Ago) 2 is the catalytic engine of mammalian RNA interference, but little is known concerning the regulation of Ago2 by cell-signaling pathways...
  50. ncbi [Antineovascular gene therapy by Ago2 knockdown]
    Kentaro Hatanaka
    Department of Medical Biochemistry, Graduate School of Pharmaceutical Sciences, University of Shizuoka, Shizouka, Japan
    Yakugaku Zasshi 128:1567-75. 2008
    ..In the present study, we optimized PCL for siRNA transfection and used it to determine the role of Argonaute2 (Ago2), a main constitution protein of RNA-induced silencing complex (RISC), on angiogenesis...
  51. pmc The human Ago2 MC region does not contain an eIF4E-like mRNA cap binding motif
    Lisa N Kinch
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9050, USA
    Biol Direct 4:2. 2009
    ..A recent report by Kiriakidou et al. 1 describes an MC sequence region identified in Ago2 that displays similarity to the cap-binding motif in translation initiation factor 4E (eIF4E)...
  52. doi Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs
    Lasse Weinmann
    Laboratory of RNA Biology and Center for Integrated Protein Science Munich, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell 136:496-507. 2009
    ..Furthermore, we detect Ago2 in the nucleus of HeLa cells, and knockdown of Imp8 reduces the nuclear Ago2 pool...
  53. pmc The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing
    Shang L Lian
    Department of Oral Biology, University of Florida, Gainesville, Florida 32610 0424, USA
    RNA 15:804-13. 2009
    ..b>Ago2 binds miRNA directly and is the core component of miRNA-induced silencing complex...
  54. pmc The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins
    Daniela Lazzaretti
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    RNA 15:1059-66. 2009
    ....
  55. pmc Hsp90 regulates the function of argonaute 2 and its recruitment to stress granules and P-bodies
    Justin M Pare
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Mol Biol Cell 20:3273-84. 2009
    ..Together, our data suggest that Hsp90 is a critical modulator of Argonaute function. Moreover, we propose that Ago2 and PACT form a complex that functions at the level of SGs.
  56. pmc Functional dissection of the human TNRC6 (GW182-related) family of proteins
    David Baillat
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell Biol 29:4144-55. 2009
    ..In contrast, the C-terminal domain containing the RNA recognition motif plays a critical role in the silencing mediated by the TNRC6B protein...
  57. pmc Cellular microRNA and P bodies modulate host-HIV-1 interactions
    Robin Nathans
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 34:696-709. 2009
    ..Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses...
  58. pmc Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation
    Marc R Fabian
    Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada
    Mol Cell 35:868-80. 2009
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  59. pmc HSP90 protein stabilizes unloaded argonaute complexes and microscopic P-bodies in human cells
    Michael Johnston
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 21:1462-9. 2010
    ..Our data suggests that HSP90 protein chaperones Argonautes before binding RNA and may facilitate efficient loading of small RNA...
  60. pmc High-risk myeloma is associated with global elevation of miRNAs and overexpression of EIF2C2/AGO2
    Yiming Zhou
    Donna D and Donald M Lambert Laboratory for Myeloma Genetics, Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Proc Natl Acad Sci U S A 107:7904-9. 2010
    ..01) and proliferation index (P < 0.05). Increased expression of EIF2C2/AGO2, a master regulator of the maturation and function of miRNAs and a component of the 70-gene mRNA risk model, ..
  61. pmc Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma
    Jason D Arroyo
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Proc Natl Acad Sci U S A 108:5003-8. 2011
    ..Further characterization revealed that Argonaute2 (Ago2), the key effector protein of miRNA-mediated silencing, was present in human plasma and eluted with plasma miRNAs ..
  62. doi Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and thereby abrogation of nonsense-mediated mRNA decay require the cap-associating activity of Ago2
    Junho Choe
    School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
    FEBS Lett 585:2682-7. 2011
    ..Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is ..
  63. pmc Retroviral GAG proteins recruit AGO2 on viral RNAs without affecting RNA accumulation and translation
    Manuella Bouttier
    Institut de Génétique Moléculaire de Montpellier UMR 5535 CNRS, 1919 Route de Mende, F 34293 Montpellier cedex 5, France
    Nucleic Acids Res 40:775-86. 2012
    ..of cellular translation, this recognition is thought to tether the RNAi machinery, in particular Argonaute 2 (AGO2) on viral messengers and eventually to modulate virus replication...
  64. pmc Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2
    Connie Wu
    Graduate Program in Biochemistry, Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111, USA
    Mol Cell Biol 31:4760-74. 2011
    ..I collagen prolyl-4-hydroxylase [C-P4H(I)], which leads to prolyl-hydroxylation and accumulation of Argonaute2 (Ago2), a critical component of the RNA-induced silencing complex (RISC)...
  65. doi Senescence is an endogenous trigger for microRNA-directed transcriptional gene silencing in human cells
    Moussa Benhamed
    Institut Pasteur, Nuclear Organisation and Oncogenesis Unit, Department of Cell Biology and Infection, F 75015 Paris, France
    Nat Cell Biol 14:266-75. 2012
    ..Here, we demonstrate that AGO2, RB1 and microRNAs (miRNAs), as exemplified here by let-7, physically and functionally interact to repress RB1/E2F-..
  66. pmc Quantitative functions of Argonaute proteins in mammalian development
    Dongmei Wang
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Genes Dev 26:693-704. 2012
    ..Although Ago2 is the only one with the slicer activity, it is not clear whether the slicer activity is a universally critical ..
  67. pmc The crystal structure of human Argonaute2
    Nicole T Schirle
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 336:1037-40. 2012
    ..The 2.3 angstrom resolution crystal structure of human Argonaute2 (Ago2) reveals a bilobed molecule with a central cleft for binding guide and target RNAs...
  68. pmc Slicing-independent RISC activation requires the argonaute PAZ domain
    Shuo Gu
    Department of Pediatrics, Stanford University, Stanford, CA 94305, USA
    Curr Biol 22:1536-42. 2012
    ....
  69. pmc Argonaute 2 complexes selectively protect the circulating microRNAs in cell-secreted microvesicles
    Limin Li
    Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
    PLoS ONE 7:e46957. 2012
    ..Here we show that Argonaute 2 (Ago2) complexes and microvesicles (MVs) provide specific and non-specific protection for miRNA in cell-secreted MVs, ..
  70. pmc Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets
    Shane R Horman
    Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA
    Mol Cell 50:356-67. 2013
    ..Here we report that the proto-oncogene Akt-3/PKBγ (Akt3) phosphorylates Argonaute 2 (Ago2) at S387, which downregulates cleavage and upregulates translational repression of endogenous microRNA (miRNA)-..
  71. pmc EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2
    Jia Shen
    Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Nature 497:383-7. 2013
    ..of specific tumour-suppressor-like miRNAs in response to hypoxic stress through phosphorylation of argonaute 2 (AGO2) at Tyr 393...
  72. doi In silico molecular docking analysis of the human Argonaute 2 PAZ domain reveals insights into RNA interference
    Mahmoud Kandeel
    Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelshiekh University, Kafrelshiekh, Egypt
    J Comput Aided Mol Des 27:605-14. 2013
    ..of small interfering RNA by PAZ in a computational study based on the structure of Drosophila Argonaute 2 (Ago2) PAZ domain. We have now reanalyzed these data within the view of the new available structures from human Argonauts...
  73. pmc Structural features of Argonaute-GW182 protein interactions
    Janina Pfaff
    Biochemistry Center Regensburg, Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany
    Proc Natl Acad Sci U S A 110:E3770-9. 2013
    ..Using cross-linking followed by mass spectrometry, we map the GW protein interactions with Ago2, which allows for structural modeling of Ago-GW182 interaction...
  74. pmc Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells
    Vera Huang
    Department of Urology and Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America
    PLoS Genet 9:e1003821. 2013
    ..Our findings reveal the first landscape of human Ago1-chromosomal interactions, which may play a role in the oncogenic transcriptional program of cancer cells. ..
  75. pmc Ago2 facilitates Rad51 recruitment and DNA double-strand break repair by homologous recombination
    Min Gao
    1 Laboratory of Genome Variations and Precision Biomedicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
    Cell Res 24:532-41. 2014
    ..is restricted to repair by homologous recombination (HR) and that it specifically relies on the effector protein Ago2 in mammalian cells...
  76. pmc miRNP:mRNA association in polyribosomes in a human neuronal cell line
    Peter T Nelson
    Department of Pathology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    RNA 10:387-94. 2004
    ..Here we describe that in a human neuronal cell line, the miRNP proteins eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes...
  77. pmc Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells
    Julia Höck
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    EMBO Rep 8:1052-60. 2007
    ..Here, we analysed Ago1- and Ago2-containing protein complexes in human cells...
  78. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..Our biochemical analysis revealed that Ago2 is present in a pre-miRNA processing complex that is able to transfer the miRNA into a target-mRNA cleaving complex...
  79. ncbi RNA helicase A interacts with RISC in human cells and functions in RISC loading
    G Brett Robb
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 26:523-37. 2007
    ..Minimally, the action of RISC requires the endonucleolytic slicer activity of Argonaute2 (Ago2) directed to RNA targets whose sequences are complementary to RISC-incorporated small RNA...
  80. pmc Adrenal hypoplasia congenita with hypogonadotropic hypogonadism: evidence that DAX-1 mutations lead to combined hypothalmic and pituitary defects in gonadotropin production
    R L Habiby
    Division of Endocrinology, Metabolism, and Molecular Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Clin Invest 98:1055-62. 1996
    ..follicle-stimulating hormone (FSH), and free-alpha-subunit (FAS) levels were determined during 24 h of frequent (q10 min) venous sampling. In patient MH, baseline LH levels were low, but FAS levels were within the normal range...
  81. ncbi Non-random chromosomal rearrangements in pancreatic cancer cell lines identified by spectral karyotyping
    V Sirivatanauksorn
    Imperial Cancer Research Fund Molecular Oncology Unit, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom
    Int J Cancer 91:350-8. 2001
    ..recurrent structural aberrations, all of them unbalanced: most frequently i(5)(p10), del(11)(q23), i(12)(p10), i(1)(q10), del(7)(q22) and del(10)(p11)...
  82. ncbi Relationships between body temperature, thermal conductance, Q10 and energy metabolism during daily torpor and hibernation in rodents
    G K Snyder
    Department of Environmental, Population and Organismic Biology, University of Colorado, Boulder 80309
    J Comp Physiol B 159:667-75. 1990
    ..We suggest that the controversy has been confused by using Q10 to evaluate the relationship of M and TB in endotherms.(ABSTRACT TRUNCATED AT 250 WORDS)
  83. ncbi [Benign infantile mitochondrial myopathy caused by reversible cytochrome c oxidase deficiency]
    H Wada
    Department of Pediatrics, Kobe University School of Medicine
    No To Hattatsu 28:443-7. 1996
    ..She received carnitine and coenzyme Q10 supplementation from the age of 3 months and abnormal blood lactate values declined to near normal values during ..
  84. ncbi Reduction of metabolism during hibernation and daily torpor in mammals and birds: temperature effect or physiological inhibition?
    F Geiser
    Department of Zoology, University of Washington, Seattle 98195
    J Comp Physiol B 158:25-37. 1988
    ..the reduction in energy metabolism during torpor in endotherms is strictly a physical effect of temperature (Q10) or whether it involves an additional metabolic inhibition...
  85. ncbi Suppression of the hydrazine-induced formation of megamitochondria in the rat liver by coenzyme Q10
    K Adachi
    Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Japan
    Toxicol Pathol 23:667-76. 1995
    The effects of coenzyme Q10 (CoQ10) on the hydrazine-induced changes in the structure of mitochondria and those in antioxidant systems of the liver were investigated using rats as experimental animals...
  86. ncbi [Birth defects incidence in children from single and twin pregnancies in the Czech Republic--current data]
    A Sipek
    Oddĕlení lékarské genetiky, Fakultní Thomayerova nemocnice, Praha
    Ceska Gynekol 74:369-82. 2009
    ..An assessment of total numbers and relative incidences of birth defects in births according to Tenth Revision of International Classification of Diseases (ICD-10)...
  87. ncbi Temperature dependency of cupular mechanics and hair cell frequency selectivity in the fish canal lateral line organ
    J Esther
    Department of Neurobiophysics, Graduate School of Behavioural and Cognitive Neurosciences, University of Groningen, The Netherlands
    J Comp Physiol A 186:949-56. 2000
    ..Cut-off frequencies of hair cell frequency selectivity were found to depend on temperature with a Q10 of 1.75, ranging from 116 Hz (4 degrees C) to 290 Hz (20 degrees C), as established in the ruffe...
  88. ncbi Ubiquinone (coenzyme q10) and mitochondria in oxidative stress of parkinson's disease
    M Ebadi
    Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota School of Medicine and Health Sciences, Grand Forks, N Dak 58203 2817, USA
    Biol Signals Recept 10:224-53. 2001
    ..complexes located in the mitochondrial inner membrane that contain flavins (FMN, FAD), quinoid compounds (coenzyme Q10, CoQ10) and transition metal compounds (iron-sulfur clusters, hemes, protein-bound copper)...
  89. pmc Seasonal variation in CO2 efflux of stems and branches of Norway spruce trees
    Manuel Acosta
    Laboratory of Plants Ecological Physiology, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Porici 3b, 603 00 Brno, The Czech Republic
    Ann Bot 101:469-77. 2008
    ..in order (1) to evaluate the influence of temperature on woody tissue CO2 efflux with special focus on variation in Q10 (change in respiration rate resulting from a 10 degrees C increase in temperature) within and between seasons, and (..
  90. ncbi Reduction of metabolic rate and thermoregulation during daily torpor
    X Song
    Department of Zoology, University of New England, Armidale, NSW, Australia
    J Comp Physiol B 165:291-7. 1995
    ..74). The Q10 for metabolic rate was between 2 and 3 over the body temperature range of 16 to 32 degrees C...
  91. ncbi Neurochemical and thermal control of surfactant secretion by alveolar type II cells isolated from the marsupial, Sminthopsis crassicaudata
    C J Ormond
    Department of Environmental Biology, University of Adelaide, Australia
    J Comp Physiol B 171:223-30. 2001
    ..change in secretory rate between 37 degrees C and 18 degrees C was less than expected if due to temperature alone (Q10= 1.4)...
  92. ncbi The latency of the response of Limulus photoreceptors to inositol trisphosphate lacks the calcium-sensitivity of that to light
    R Payne
    Department of Zoology, University of Maryland, College Park 20742
    J Comp Physiol A 170:311-6. 1992
    ..Reducing the temperature lengthened the latency of the response to light (Q10 approximately 3.2 between 7 and 22 degrees C) more than that to inositol trisphosphate (Q10 approximately 2.3)...
  93. ncbi Impaired plasma antioxidative defense and increased nontransferrin-bound iron during high-dose chemotherapy and radiochemotherapy preceding bone marrow transplantation
    M Dürken
    Department of Pediatric Hematology and Oncology, University of Hamburg, Hamburg, Germany
    Free Radic Biol Med 28:887-94. 2000
    ..TRAP) and single plasma antioxidants (uric acid, sulfhydryl groups, alpha-tocopherol, ubiquinone-10/total coenzyme-Q10 ratio, ascorbate, and bilirubin) every 12 h during high-dose chemotherapy and radiochemotherapy preceding bone ..
  94. ncbi Temperature and the temporal resolving power of fly photoreceptors
    B Tatler
    Department of Zoology, University of Cambridge, UK
    J Comp Physiol A 186:399-407. 2000
    ..When light adapted, the corner frequency increases from 53 Hz to 119 Hz with a Q10 of 1.9, and when dark adapted from 8 Hz to 32 Hz with a Q10 of 3.0...
  95. ncbi Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain
    Andreas Lingel
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 426:465-9. 2003
    ..The Argonaute 2 protein (Ago2) is a critical component of RISC...
  96. ncbi In vivo and in situ rhizosphere respiration in Acer saccharum and Betula alleghaniensis seedlings grown in contrasting light regimes
    Sylvain Delagrange
    Groupe de Recherche en Ecologie Forestière inter universitaire, Universite du Quebec a Montreal, C P 8888, Succ Centre Ville, Montreal, Quebec, Canada, H3P 3P8
    Tree Physiol 26:925-34. 2006
    ..specific rhizosphere respiration, temperature corrections were made with either species-specific coefficients (Q10) based on the observed change in respiration rate between 15 and 21 degrees C or an arbitrarily assigned Q10 of 2...
  97. ncbi [Birth defects in the Czech Republic in 1994--2007]
    A Sipek
    Oddĕlení lékarské genetiky, Fakultní Thomayerova nemocnice, Praha
    Ceska Gynekol 74:31-44. 2009
    ..An assessment of total numbers and relative incidences of birth defects in births according to Tenth Revision of International Classification of Diseases (ICD-10)...
  98. ncbi Central auditory neurophysiology of a sound-producing fish: the mesencephalon of Pollimyrus isidori (Mormyridae)
    J D Crawford
    Parmly Hearing Institute, Loyola University, Chicago, IL 60626
    J Comp Physiol A 172:139-52. 1993
    ..BSs were in the range of 0 to -35 dB (re. 1.0 dyne/cm2). Many of the neurons were tuned (Q10 dB: 2-6), and had BFs in the range of 100-300 Hz where the animal's sounds have their peak energy...
  99. ncbi Effects of simvastatin on blood lipids, vitamin E, coenzyme Q10 levels and left ventricular function in humans
    D M Colquhoun
    Greenslopes Private Hospital and The Wesley Hospital, Brisbane, Australia
    Eur J Clin Invest 35:251-8. 2005
    As statin therapy has been reported to reduce antioxidants such as vitamin E and coenzyme Q10 and there are indications that this reduction may cause impairment of left ventricular function (LVF), we studied the influence of simvastatin ..
  100. doi Enhancement of shortening velocity, power, and acto-myosin crossbridge (CB) kinetics following long-term treatment with propionyl-L-carnitine, coenzyme Q10, and omega-3 fatty acids in BIO TO-2 cardiomyopathic Syrian hamsters papillary muscle
    Romina Vargiu
    Department of Science Applied to Biosystems, Section of Physiology and Human Nutrition, University of Cagliari, Italy
    Biofactors 36:229-39. 2010
    ....
  101. ncbi Effect of coenzyme Q10 supplementation on mitochondrial function after myocardial ischemia reperfusion
    Juan A Crestanello
    Division of Cardiothoracic Surgery, University of Maryland Medical System, Baltimore, Maryland, USA
    J Surg Res 102:221-8. 2002
    Coenzyme Q10 (CoQ10) protects myocardium from ischemia-reperfusion (IR) injury as evidenced by improved recovery of mechanical function, ATP, and phosphocreatine during reperfusion...

Research Grants65

  1. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2012
    Coenzyme Q10 (CoQ10) is a small lipophillic molecule composed of a benzoquinone ring and a hydrophobic isoprenoid tail and is present in virtually all cell membranes...
  2. CoEnzyme Q10 in Statin Myopathy
    PAUL DAVIS THOMPSON; Fiscal Year: 2010
    ..synthesis early in the mevalonate pathway thereby reducing other end products including ubiquinone or Coenzyme Q10 (CoQ10)...
  3. Catarina M Quinzii; Fiscal Year: 2014
    DESCRIPTION (Provided by Applicant): Coenzyme Q10 (CoQ10) is a small lipophilic molecule composed of a benzoquinone ring and a hydrophobic isoprenoid tail which is present in virtually all cell membranes...
  4. Biomarkers of Oxidative Stress in Parkinson's Disease
    M Flint Beal; Fiscal Year: 2010
    ..We are presently carrying out a phase III clinical trial of the antioxidant nutritional supplement coenzyme Q10 (CoQ10) in PD...
  5. Jack D Keene; Fiscal Year: 2015
    ..quantify dynamic changes in RNAs associated with HuR (early response gene mRNAs), TIAR (stress granule RNAs) and AGO2/RISC (processing "P" body RNAs)...
  6. Anti-oxidant treatment of muscle wasting and fatigue in tumor-bearing mice
    YVONNE YUMIKO CLARK; Fiscal Year: 2012
    ..Coenzyme Q10 (coQ10) is a naturally occurring anti-oxidant in mitochondria of cells that is depleted during the inflammatory ..
  7. FREE RADICAL LIPID PEROXIDATION DAMAGE
    ALOYS TAPPEL; Fiscal Year: 1991
    ..for which antioxygenic capacity has not been adequately determined, including beta-carotene, uric acid, coenzyme Q10, and (+)-catechin. 5...
  8. PREQUEL Study In PRE-manifest HD of CoQ10/UbiquinonE Leading to Preventive Trials
    Christopher A Ross; Fiscal Year: 2010
    ..CoEnzyme Q10 (CoQ) has emerged as one of the leading therapeutic candidates for neuroprotection in manifest HD, with strong near-..
  9. Effects of Antioxidant Supplementation during Breast Cancer Adjuvant Therapy
    Heather Greenlee; Fiscal Year: 2013
    ..For example, coenzyme Q10 (C0QIO), a lipid soluble antioxidant, has been hypothesized to decrease doxorubicin-induced cardiotoxicity...
  10. Coenzyme Q10 in Huntingtons Disease
    Merit E Cudkowicz; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The study objective is to determine the efficacy of coenzyme Q10 (CoQ) in Huntington's disease (HD)...
  11. Michael P McDermott; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): The study objective is to determine the efficacy of coenzyme Q10 (CoQ) in Huntington's disease (HD)...
  12. microRNA Uncoupling of Protein and Transcript Expression in Liver Regeneration
    CLIFFORD JOHN STEER; Fiscal Year: 2010
    ..This will be done by specific knockdown of critical components of Dicer in mina processing and Ago2 in the RNA Induced Silencing Complex (RISC)...
  13. Tatsuya Kobayashi; Fiscal Year: 2016
    ..and differentiation, first we will experimentally identify miRNA-regulated transcripts in chondrocytes by profiling Ago2- associated RNAs...
  14. Daniel Cifuentes; Fiscal Year: 2014
    ..a novel processing pathway where miRNA maturation skips the Dicer step and instead enters into an alternative Ago2-dependent pathway. This finding challenges a long-held assumption that Dicer is essential for miRNA maturation...
  15. Cardiac specific RNA Helicase is critical for normal cardiomyocyte proliferation
    RONALD LEE NEPPL; Fiscal Year: 2012
    ....
  16. Circadian regulation of microRNA biogenesis and function
    Peter W Vanderklish; Fiscal Year: 2012
    ..2) "Antagomirs" and reporter constructs will be used to determine whether regulation of Drosha, Dicer and Ago2 by RBM3 involves direct effects on their translation, or feedback mechanism involving miRNAs that are regulated by ..
  17. Phillip A Sharp; Fiscal Year: 2016
    ..First, we will further investigate the nature of factors targeting Argonaute 2 (Ago2) to specific RNA sequences in Dicer null cells...
  18. MicroRNA networks in synaptic plasticity
    Richard H Goodman; Fiscal Year: 2010
    ..miR132 and miR134 by immunopurifying RNA-induced silencing complexes (RISC) containing an epitope-tagged version of Ago2. A few targets of these microRNAs have been identified using various prediction algorithms, but most remain to be ..
  19. Elucidating Nuclear Argonaute Function
    Timothy J Kelly; Fiscal Year: 2013
    ..Of the four mammalian-encoded Ago proteins, Ago2 plays a central role in both miRNA and siRNA mediated mRNA regulation and has been suggested to bind to chromatin ..
  20. Carl D Novina; Fiscal Year: 2015
    ..To identify miRNA- repressed mRNAs affected by eIF4E perturbation, we will perform microarray analysis of Ago2 immunopreciptiates...
  21. Biochemical Studies of Drosophila RNA-induced Silencing Complex
    Qinghua Liu; Fiscal Year: 2010
    ..A minimal RISC can form between recombinant Ago2, the ribonuclease of RISC, and single stranded siRNA, but not with double-stranded (ds) siRNA...
  22. Role of Huntington's Disease Protein in Post-Transcriptional Gene Silencing
    Naoko Tanese; Fiscal Year: 2013
    ..The association of Htt with Ago2 and the localization of Htt to P-bodies provide new and compelling evidence connecting Htt with the post-..
  23. Combinatorial microRNA regulation of cardiac transcription factors
    Richard H Goodman; Fiscal Year: 2013
    ..To elucidate the mechanism underlying the interdependency of miR-1 and miR-133a binding, we will target Ago2, a core component of the RNA induced silencing complex (RISC), to the mutated Hand2 miR-1 or miR-133a MREs to ..
  24. FMRP-regulated association of specific miRNAs with RISC
    BART RUSSELL ANDERSON; Fiscal Year: 2013
    ..FMRP target, GluA1 mRNA;and 2) Assess the extent to which Fmr1-/- alters the association of the RISC protein Ago2 with specific miRNAs at the synapse...
  25. IAN JOHN MACRAE; Fiscal Year: 2016
    ..proposed research will provide high- resolution structures and functional analyses of full-length human Argonaute2 (Ago2)...
  26. Stefania Nicoli; Fiscal Year: 2014
    ..In parallel, I will generate an endothelial cell-specific transgenic line expressing myc tagged Argonaute 2 (Ago2)...
  27. Richard H Goodman; Fiscal Year: 2016
    ..2) Determine whether heparin-binding (Hb)-EGF and the CDK inhibitor, p21, two miR-132 targets identified in Ago2-immunopurification assays, contribute to miR-132 effects on neuronal survival, morphology, and function...
  28. Talat Alp Ikizler; Fiscal Year: 2016
    ..antagonist (IL-1ra), Fish Oil (EPA+DHA), Vitamin E (gamma-tocopherol), alpha lipoic acid (ALA) and CoEnyzme Q10 in studies ranging from 14 patients to 300 patients...
  29. Darrin V Bann; Fiscal Year: 2015
    ..are formed at discrete sites in the cytoplasm that associate with host mRNA processing factors Yb1, Mov10, and Ago2. We envision that the MMTV RNA might be trafficking to these sites to avoid translation machinery, instead being ..
  30. Cell-based Genomic Analysis in Mouse Models of Rett Syndrome
    Z Josh Huang; Fiscal Year: 2010
    ..cNIP by Cre/loxP-regulated expression of epitope-tagged polyA- binding protein (PABP, for mRNA) or argonaute2 (AGO2, for miRNA) in specific cell types...
  31. Regulation of the Drosophila Fragile X Protein by siRNA Pathway Components
    Thomas A Jongens; Fiscal Year: 2010
    ..Loss of the core members of this pathway (AGO2, R2D2 and Dicer-2) leads to significant upregulation of dFMR1 protein levels in the germline and nervous system ..
  32. Guang Peng; Fiscal Year: 2015
    ..will lay the foundation for my independent research career, is to understand the novel nuclear function of human Ago2, a core protein in RNA interference pathways, in HR repair and genome maintenance...
  33. Understanding and Targeting the DNA Repair Network in Cancer
    Guang Peng; Fiscal Year: 2012
    ..will lay the foundation for my independent research career, is to understand the novel nuclear function of human Ago2, a core protein in RNA interference pathways, in HR repair and genome maintenance...
  34. Benefit of coenzyme Q10 in patients on statins
    Giuseppe Caso; Fiscal Year: 2010
    ..may, in part, be a consequence of a mitochondrial deficit resulting from the inhibition of synthesis of coenzyme Q10 (coQ10), an essential cofactor for mitochondrial electron transport systems, which shares the same biosynthetic ..
  35. Effect of CoQ10 on Mild-to-Moderate Muscle Symptoms of Statin Users.
    Catherine Buettner; Fiscal Year: 2013
    ..Some evidence suggests that use of a dietary supplement, coenzyme Q10, may improve muscle symptoms in statin users...
  36. RNAi as a modulator of arbovirus vector competence in transgenic Aedes aegypti
    ALEXANDER WILLIAM EDUARD FRANZ; Fiscal Year: 2010
    ..aegypti when they are not controlled by the mosquito's RNAi response. The proposed research will help to reveal the impact of RNAi as an antiviral defense mechanism in the midgut of Ae. aegypti. ..
  37. Continuation of NET-PD Trials at the University of Kansas Medical Center
    Rajesh Pahwa; Fiscal Year: 2013
    ..As part of this initiative 2 futility trials examining creatine, minocycline, co-enzyme Q10 and GPI-1485 were conducted as a unique approach for PD studies, to quickly identify potentially neuroprotective ..
  38. Peter A Beal; Fiscal Year: 2016
    ..First, we will identify modifications to the guid stand 3'end that modulate affinity for the Ago2 PAZ domain...
  39. Enabling Xenopus oocytes and embryos to perform RNAi
    Michael D Sheets; Fiscal Year: 2013
    ..Recently, we discovered that Xenopus oocytes and early embryos lack functional Ago2, the nuclease containing protein that is guided to targeted mRNAs by siRNAs, which would explain why these cells ..
  40. Silencing Suppression Mechanism of a Herpes Simplex Virus Virion Component
    NIL CELEBI CHERUKURI; Fiscal Year: 2009
    ..with known components of the RNA-induced silencing complex (RISC), including TRBP (a PACT homolog), Argonaute 2 (Ago2), and Dicer...
  41. Effects of Coenzyme Q10 in Parkinson Disease-Phase 3
    M Flint Beal; Fiscal Year: 2010
    ..II trial, our group demonstrated that treatment of patients with early, untreated PD with high dosages of coenzyme Q10 (300, 600 and 1200 mg/d) was safe and well tolerated and that there was a positive trend for treatment to slow the ..
  42. Neuroprotection in Parkinson Disease: Clinical Center
    Rodger Elble; Fiscal Year: 2013
    ..SIUSM was a productive site in the initial two futility studies of creatine, coenzyme Q10, minocycline, and GPI 1485 and is a high-enrolling site for the ongoing Phase 3 randomized, double-blinded and ..
  43. COLLABORATIVE STUDY OF 1,3 BUTADIENE BIOMARKERS
    Thomas Smith; Fiscal Year: 2002
    ..05) to detect a q10 percent difference in slopes among subgroups...
  44. Mitochondrial Metabiolites to Treat Parkinson's Disease
    Bruce Ames; Fiscal Year: 2005
    ..There is evidence that some mitochondrial metabolites may protect against PD. For example, coenzyme Q10 (CoQ) has been shown to protect against PD in clinical trials in humans; and ALCAR has been shown to protect ..
  45. Microarrays for Alzheimer's Disease Mouse Models
    P Reddy; Fiscal Year: 2003
    ..mice and age-matched wild-type mice with mitochondrial antioxidants such as lipoic acid, melatonin and co-enzyme Q10 to determine whether mitochondrial antioxidants can rescue cognitive deficits, reduce mRNA expression, and ..
  46. Mitochondrial Nutrients Protect RPE from Oxidant Damage
    Bruce Ames; Fiscal Year: 2006
    ..contributes to the retinal degeneration observed in AMD and that compounds such as alpha-lipoic acid, coenzyme Q10, and acetyl-L-carnitine, which protect mitochondria against oxidative insult, may prevent or lessen oxidant induced ..
  47. Julie H Carter; Fiscal Year: 2014
    ..Study 2 (FS-TOO) which evaluated the therapeutic potential of four compounds (creatine, minocycline, coenzyme Q10 (CoQ10), and GPI-1485) [5, 6]...
  48. COENZYME Q10 AND REMACEMIDE--EVALUATION IN HD
    Karl Kieburtz; Fiscal Year: 2000
    ..Interventions that buttress mitochondrial energy metabolism and scavenge free radicals, such as co-enzyme Q10, or that block NMDA receptor activation, such as remacemide hydrochloride have been shown to attenuate these HD-..
  49. COENZYME Q10 IN EARLY PARKINSONS DISEASE
    Clifford Shults; Fiscal Year: 2000
    ..Coenzyme Q10 (CoQ10) is the electron acceptor for complex I and a potent antioxidant...
  50. Characterization of TRBP-containing complexes
    Ramin Shiekhattar; Fiscal Year: 2009
    ..the detailed functional analysis of the association of Dicer/TRBP complex with the effector complex containing the Ago2 subunit and delineates the role of individual proteins in the functioning of the posttranscriptional gene silencing...
  51. Mentored Patient Oriented Research Career Development
    Diana Escolar; Fiscal Year: 2005
    ..Aim 2 is to conduct a double-blind, placebo- controlled clinical trial of coenzyme Q10 (CoQ10) in patients with DMD to assess its effects on: a) muscle strength, measured by QMT, compound Medical ..
  52. Coordination and Statistics for CoQ10 in PD in Phase 3
    David Oakes; Fiscal Year: 2009
    ..will provide Coordination, Data Management and Statistical support for the conduct of a clinical trial of Coenzyme Q10 in early Parkinson's disease to be led by Dr...
  53. HUNTINGTON PROTEIN AND THE HUNTINGTON'S DISEASE MUTATION
    Steven Hersch; Fiscal Year: 2003
    ..We have preliminary evidence that coenzyme Q10, an antioxidant, and creatine, an energy buffer, can extend survival in transgenic models of Huntington's disease...
  54. Cardiac Hypertrophy Due to Oxidative Stress Insulin
    Thunder Jalili; Fiscal Year: 2006
    ..Finally we shall determine if a dietary approach using supplemented antioxidant minerals and Coenzyme Q10 can be equally successful in reducing cardiac oxidative stress, PKC activation, and hypertrophy...
  55. Clinical Trial of High Dose CoQ10 in ALS-QALS-=STAT
    John Thompson; Fiscal Year: 2007
    ..QALS, a two-stage, phase II, randomized, placebo-controlled, double-blind, multi-center clinical trial of Coenzyme Q10 against placebo (target enrollment 185 patients at 18 clinical sites)...
  56. Interactions of Coenzyme Q10 with plasma tocopherols and their role in breast can
    Robert Cooney; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): Coenzyme Q10 is of critical importance to mitochondrial function and its deficiency is associated with impaired ATP synthesis, apoptosis, and immune function...
  57. Regulation of Novel Mitochondrial Uncoupling Proteins
    Keith Garlid; Fiscal Year: 2004
    ..of the hypothesis that fatty acid-induced proton transport by UCP2 exhibits an absolute requirement for coenzyme Q10. Aim 3 will utilize sensitive and precise measurements of oxygen consumption to detect UCP2 activity in Beta-cell ..
  58. CLINICAL RESEARCH CENTER FOR NEUROMUSCULAR DISEASE
    Salvatore DiMauro; Fiscal Year: 2007
    ....
  59. PINEAL OPIOID RECEPTORS & ANALGESIC ACTION OF MELATONIN
    Manuchair Ebadi; Fiscal Year: 2002
    ..Therefore, future research must synthesize highly efficacious melatonin analogues capable of providing maximum analgesia and hopefully being devoid of addiction liability now associated with currently available narcotics. ..
  60. UBIQUINONE AND MITOCHONDRIAL OXIDATIVE DISORDERS OF AGIN
    Manuchair Ebadi; Fiscal Year: 2003
    ..A decline in aerobic metabolism has been observed in aging tissues. Ubiquinone(coenzyme Q10), in addition to its function as an electron and proton carrier in mitochondrial electron transport coupled to ATP ..
  61. Metal-Mediated Routes to Biaryls
    Bruce Lipshutz; Fiscal Year: 2007
    ..A particular, albeit representative, application of a novel substituted cyclo-NOBIN will be studied for selected asymmetric aldol reactions. ..
  62. MITOCHONDRIAL DNA ANALYSIS IN CYCLIC VOMITING SYNDROME
    Richard Boles; Fiscal Year: 2002
    ..An extensive amount of clinical and laboratory data will be collected in all patients, allowing for the comparison of CVS sufferers with and without mtDNA mutations. ..
  63. Development of New Therapies for Huntington's Disease
    M Beal; Fiscal Year: 2008
    ..We believe that these studies will provide critical pre-clinical data to determine the agents, which have the most promise for use as neuroprotective agents in clinical drug trials in HD patients. ..