CHIP

Summary

Gene Symbol: CHIP
Description: STIP1 homology and U-box containing protein 1
Alias: CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, RING-type E3 ubiquitin transferase CHIP, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase, antigen NY-CO-7, carboxy terminus of Hsp70-interacting protein, heat shock protein A binding protein 2 (c-terminal), serologically defined colon cancer antigen 7
Species: human

Top Publications

  1. ncbi Co-chaperone CHIP promotes aggregation of ataxin-1
    Jung Young Choi
    Protein Therapeutics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun dong, Yuseong Gu, Daejeon 305 333, South Korea
    Mol Cell Neurosci 34:69-79. 2007
  2. ncbi CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation
    Leonard Petrucelli
    Mayo Clinic, Jacksonville, FL 32224, USA
    Hum Mol Genet 13:703-14. 2004
  3. ncbi CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity
    Yuzuru Imai
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute, Saitama 351 0198, Japan
    Mol Cell 10:55-67. 2002
  4. pmc C-terminus of heat shock protein 70-interacting protein facilitates degradation of apoptosis signal-regulating kinase 1 and inhibits apoptosis signal-regulating kinase 1-dependent apoptosis
    Jae Ryoung Hwang
    Carolina Cardiovascular Biology Center and Department of Medicine, 8200 Medical Biomolecular Research Building, University of North Carolina, Chapel Hill, NC 27599, USA
    Cell Stress Chaperones 10:147-56. 2005
  5. pmc Identification of CHIP as a novel causative gene for autosomal recessive cerebellar ataxia
    Yuting Shi
    Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    PLoS ONE 8:e81884. 2013
  6. ncbi The chaperone-associated ubiquitin ligase CHIP is able to target p53 for proteasomal degradation
    Claudia Esser
    Institute for Cell Biology and Bonner Forum Biomedizin, Rheinische Friedrich Wilhelms University Bonn, Ulrich Haberland Str 61a, D 53121 Bonn, Germany
    J Biol Chem 280:27443-8. 2005
  7. ncbi In vivo evidence of CHIP up-regulation attenuating tau aggregation
    Naruhiko Sahara
    Laboratory for Alzheimer s Disease, RIKEN Brain Science Institute, Saitama, Japan
    J Neurochem 94:1254-63. 2005
  8. ncbi Accumulation of the authentic parkin substrate aminoacyl-tRNA synthetase cofactor, p38/JTV-1, leads to catecholaminergic cell death
    Han Seok Ko
    Institute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 25:7968-78. 2005
  9. ncbi CHIP (carboxyl terminus of Hsc70-interacting protein) promotes basal and geldanamycin-induced degradation of estrogen receptor-alpha
    Meiyun Fan
    Medical Sciences, Indiana University School of Medicine, 302 Jordan Hall, 1001 East 3rd Street, Bloomington, IN 47405 4401, USA
    Mol Endocrinol 19:2901-14. 2005
  10. pmc Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression
    Ssang Taek Lim
    Department of Reproductive Medicine, University of California San Diego, Moores Cancer Center, La Jolla, CA 92093, USA
    J Cell Biol 197:907-19. 2012

Detail Information

Publications268 found, 100 shown here

  1. ncbi Co-chaperone CHIP promotes aggregation of ataxin-1
    Jung Young Choi
    Protein Therapeutics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun dong, Yuseong Gu, Daejeon 305 333, South Korea
    Mol Cell Neurosci 34:69-79. 2007
    Recent studies demonstrated that co-chaperone/E3 ligase CHIP (C-terminus of hsp70-interacting protein) mediates the ubiquitylation and suppresses the aggregation of polyglutamine (polyQ) proteins, such as huntingtin or ataxin-3...
  2. ncbi CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation
    Leonard Petrucelli
    Mayo Clinic, Jacksonville, FL 32224, USA
    Hum Mol Genet 13:703-14. 2004
    ..g. tau and alpha-synuclein respectively). Here we report that CHIP, an ubiquitin ligase that interacts directly with Hsp70/90, induces ubiquitination of the microtubule associated ..
  3. ncbi CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity
    Yuzuru Imai
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute, Saitama 351 0198, Japan
    Mol Cell 10:55-67. 2002
    ..Here, we show that CHIP, Hsp70, Parkin, and Pael-R formed a complex in vitro and in vivo...
  4. pmc C-terminus of heat shock protein 70-interacting protein facilitates degradation of apoptosis signal-regulating kinase 1 and inhibits apoptosis signal-regulating kinase 1-dependent apoptosis
    Jae Ryoung Hwang
    Carolina Cardiovascular Biology Center and Department of Medicine, 8200 Medical Biomolecular Research Building, University of North Carolina, Chapel Hill, NC 27599, USA
    Cell Stress Chaperones 10:147-56. 2005
    ..Among the proteins that determine Hsp70 function, CHIP (C-terminus of Hsp70-interacting protein) is a cochaperone and ubiquitin ligase that interacts with Hsp70 through ..
  5. pmc Identification of CHIP as a novel causative gene for autosomal recessive cerebellar ataxia
    Yuting Shi
    Department of Neurology, Xiangya Hospital, Central South University, Changsha, China
    PLoS ONE 8:e81884. 2013
    ..493CT) in CHIP (NM_005861) in this family. Using Sanger sequencing, we also identified two compound heterozygous mutations (c...
  6. ncbi The chaperone-associated ubiquitin ligase CHIP is able to target p53 for proteasomal degradation
    Claudia Esser
    Institute for Cell Biology and Bonner Forum Biomedizin, Rheinische Friedrich Wilhelms University Bonn, Ulrich Haberland Str 61a, D 53121 Bonn, Germany
    J Biol Chem 280:27443-8. 2005
    ..Here we show that the chaperone-associated ubiquitin ligase CHIP is able to induce the proteasomal degradation of p53...
  7. ncbi In vivo evidence of CHIP up-regulation attenuating tau aggregation
    Naruhiko Sahara
    Laboratory for Alzheimer s Disease, RIKEN Brain Science Institute, Saitama, Japan
    J Neurochem 94:1254-63. 2005
    The carboxyl terminus of heat-shock cognate (Hsc)70-interacting protein (CHIP) is a ubiquitin E3 ligase that can collaborate with molecular chaperones to facilitate protein folding and prevent protein aggregation...
  8. ncbi Accumulation of the authentic parkin substrate aminoacyl-tRNA synthetase cofactor, p38/JTV-1, leads to catecholaminergic cell death
    Han Seok Ko
    Institute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 25:7968-78. 2005
    ..Moreover, these results have important implications for understanding the molecular mechanisms of neurodegeneration in PD...
  9. ncbi CHIP (carboxyl terminus of Hsc70-interacting protein) promotes basal and geldanamycin-induced degradation of estrogen receptor-alpha
    Meiyun Fan
    Medical Sciences, Indiana University School of Medicine, 302 Jordan Hall, 1001 East 3rd Street, Bloomington, IN 47405 4401, USA
    Mol Endocrinol 19:2901-14. 2005
    ..One potential mechanism involves CHIP, the carboxyl terminus of Hsc70-interacting protein, previously shown to target Hsp90-interacting proteins for ..
  10. pmc Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression
    Ssang Taek Lim
    Department of Reproductive Medicine, University of California San Diego, Moores Cancer Center, La Jolla, CA 92093, USA
    J Cell Biol 197:907-19. 2012
    ..1, ezrin, radixin, moesin homology) domain bound directly to GATA4 and enhanced its CHIP (C terminus of Hsp70-interacting protein) E3 ligase-dependent polyubiquitination and degradation...
  11. pmc Glucocorticoid-induced Leucine zipper 1 stimulates the epithelial sodium channel by regulating serum- and glucocorticoid-induced kinase 1 stability and subcellular localization
    Rama Soundararajan
    Division of Nephrology, Department of Medicine, University of California San Francisco, San Francisco, California 94143, USA
    J Biol Chem 285:39905-13. 2010
    ..decreases SGK1 localization to ER as well as its interaction with ER-associated E3 ubiquitin ligases, HRD1 and CHIP. GILZ1 inhibits SGK1 ubiquitinylation and subsequent proteasome-mediated degradation, thereby prolonging its half-..
  12. ncbi Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex
    Minghao Zhang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom
    Mol Cell 20:525-38. 2005
    b>CHIP is a dimeric U box E3 ubiquitin ligase that binds Hsp90 and/or Hsp70 via its TPR-domain, facilitating ubiquitylation of chaperone bound client proteins...
  13. pmc Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions
    C A Ballinger
    University of Texas Medical Branch, Division of Cardiology and Sealy Center for Molecular Cardiology, Galveston, Texas, USA
    Mol Cell Biol 19:4535-45. 1999
    ..proteins, we have identified a novel 35-kDa cytoplasmic protein, carboxyl terminus of Hsc70-interacting protein (CHIP). CHIP is highly expressed in adult striated muscle in vivo and is expressed broadly in vitro in tissue culture...
  14. pmc Ubiquitin-proteasome degradation of serum- and glucocorticoid-regulated kinase-1 (SGK-1) is mediated by the chaperone-dependent E3 ligase CHIP
    Larissa Belova
    Department of Medicine and Committee on Cancer Biology, University of Chicago, Chicago, IL 60637, USA
    Biochem J 400:235-44. 2006
    ..In the present study we report that SGK-1 forms a complex with the stress-associated E3 ligase CHIP [C-terminus of Hsc (heat-shock cognate protein) 70-interacting protein]; CHIP is required for both the ubiquitin ..
  15. ncbi Parkin interacts with LIM Kinase 1 and reduces its cofilin-phosphorylation activity via ubiquitination
    Meng K Lim
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Tokyo, Japan
    Exp Cell Res 313:2858-74. 2007
    ..LIMK1 also interacted with previously known parkin interactors Hsp70 and CHIP. Parkin enhanced LIMK1-ubiquitination in the human neuroblastoma-derived BE(2)-M17 cell line, but not in the human ..
  16. pmc Novel role of C terminus of Hsc70-interacting protein (CHIP) ubiquitin ligase on inhibiting cardiac apoptosis and dysfunction via regulating ERK5-mediated degradation of inducible cAMP early repressor
    Chang Hoon Woo
    Aab Cardiovascular Research Institute, University of Rochester, Rochester, New York 14642, USA
    FASEB J 24:4917-28. 2010
    ..A cardioprotective effect of ubiquitin (Ub) ligase of the C terminus of Hsc70-interacting protein (CHIP) on myocytes has been reported...
  17. doi Akt phosphorylation at Thr308 and Ser473 is required for CHIP-mediated ubiquitination of the kinase
    Chih Hao Su
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan
    Cell Signal 23:1824-30. 2011
    ..We show here that Thr308 and Ser473 phosphorylations prompt Akt to enter the CHIP-mediated ubiquitin-proteasome pathway...
  18. doi DNA-binding regulates site-specific ubiquitination of IRF-1
    Vivien Landré
    Cell Signalling Unit, Edinburgh Cancer Research Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Rd South, Edinburgh EH4 2XR, UK
    Biochem J 449:707-17. 2013
    ..Ubiquitin modification of full-length IRF-1 by E3 ligases such as CHIP [C-terminus of the Hsc (heat-shock cognate) 70-interacting protein] and MDM2 (murine double minute 2), which dock ..
  19. pmc The chaperone-dependent ubiquitin ligase CHIP targets HIF-1α for degradation in the presence of methylglyoxal
    Carla Figueira Bento
    Center of Ophthalmology and Vision Sciences COCV, Institute for Biomedical Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    PLoS ONE 5:e15062. 2010
    ..Moreover, we identified CHIP (Carboxy terminus of Hsp70-Interacting Protein) as the E3 ligase that ubiquitinated HIF-1α in the presence of MGO...
  20. pmc Chaperone-dependent E3 ubiquitin ligase CHIP mediates a degradative pathway for c-ErbB2/Neu
    Wanping Xu
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 99:12847-52. 2002
    ..We now show that the chaperone-binding ubiquitin ligase CHIP efficiently ubiquitinates and down-regulates ErbB2...
  21. ncbi CHIP interacts with heat shock factor 1 during heat stress
    Soo A Kim
    Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk dong, Dong Gu, Gwangju 501 759, Republic of Korea
    FEBS Lett 579:6559-63. 2005
    ..In this study, we identified the interaction of CHIP (carboxyl terminus of the heat shock cognate protein 70-interacting protein) with the N-terminus of HSF1...
  22. pmc Menin missense mutants associated with multiple endocrine neoplasia type 1 are rapidly degraded via the ubiquitin-proteasome pathway
    Hiroko Yaguchi
    Tumor Endocrinology Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Mol Cell Biol 24:6569-80. 2004
    ..wild-type menin, interact both with the molecular chaperone Hsp70 and with the Hsp70-associated ubiquitin ligase CHIP, and the overexpression of CHIP promotes the ubiquitination of the menin mutants in vivo...
  23. ncbi Association of DJ-1 and parkin mediated by pathogenic DJ-1 mutations and oxidative stress
    Darren J Moore
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Mol Genet 14:71-84. 2005
    ..The interaction of parkin with L166P DJ-1 may involve a larger protein complex that contains CHIP and Hsp70, perhaps accounting for the lack of parkin-mediated ubiquitination...
  24. doi beta-Arrestins facilitate ubiquitin-dependent degradation of apoptosis signal-regulating kinase 1 (ASK1) and attenuate H2O2-induced apoptosis
    Zhengping Zhang
    Jiangsu Province Key Laboratory for Molecular and Medicine Biotechnology, College of Life Science, Nanjing Normal University, No 1 Wenyuan Road, Nanjing, Jiangsu, PR China
    Cell Signal 21:1195-206. 2009
    ..Structurally, C-terminal domain of ASK1 is essential for beta-Arrestins and ASK1 association. We also found that CHIP is required for beta-Arrestins-induced ASK1 degradation, which suggested that beta-Arrestins function as a scaffold ..
  25. ncbi CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation
    J Jiang
    Program in Molecular Cardiology and Lineberger Comprehensive Cancer Center, The Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599 7075, USA
    J Biol Chem 276:42938-44. 2001
    ..b>CHIP (carboxyl terminus of Hsc70-interacting protein) is such a cofactor that interacts with Hsc70 and, in general, ..
  26. ncbi Cooperation of a ubiquitin domain protein and an E3 ubiquitin ligase during chaperone/proteasome coupling
    J Demand
    Abteilung für Molekulare Zellbiologie, Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
    Curr Biol 11:1569-77. 2001
    ..Molecular mechanisms underlying the cooperation of molecular chaperones with the degradation machinery remain largely enigmatic so far...
  27. pmc CHIP activates HSF1 and confers protection against apoptosis and cellular stress
    Qian Dai
    Carolina Cardiovascular Biology Center, and Department of Medicine, University of North Carolina, Chapel Hill, NC, USA
    EMBO J 22:5446-58. 2003
    ..We demonstrate that the dual function co-chaperone/ubiquitin ligase CHIP (C-terminus of Hsp70-interacting protein) regulates activation of the stress-chaperone response through induced ..
  28. ncbi CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival
    Hideki Shimura
    Department of Neurology, Harvard Medical School and Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 279:4869-76. 2004
    ..Hsc70, and its phosphorylation is a recognition requirement for the addition of ubiquitin (Ub) by the E3 Ub ligase CHIP (carboxyl terminus of the Hsc70-interacting protein) and the E2 conjugating enzyme UbcH5B...
  29. pmc CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription
    Linyu Li
    Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology and School of Medicine, Tsinghua University, Beijing 100084, People s Republic of China
    Mol Cell Biol 24:856-64. 2004
    ..the regulation of SMAD-mediated signaling, by which the Smad1 protein level is controlled through expression of the CHIP protein. CHIP is a U-box-dependent E3 ubiquitin ligase, previously identified as a cochaperon protein...
  30. ncbi An androgen receptor NH2-terminal conserved motif interacts with the COOH terminus of the Hsp70-interacting protein (CHIP)
    Bin He
    Laboratories for Reproductive Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 279:30643-53. 2004
    ..was suggested from a yeast two-hybrid screen that identified the COOH terminus of the Hsp70-interacting protein (CHIP) as a binding partner...
  31. pmc The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator
    Simon Alberti
    Institut für Zellbiologie and Bonner Forum Biomedizin, Rheinische Friedrich Wilhelms Universitat Bonn, D 53121 Bonn, Germany
    Mol Biol Cell 15:4003-10. 2004
    The CHIP ubiquitin ligase turns molecular chaperones into protein degradation factors...
  32. ncbi U-box protein carboxyl terminus of Hsc70-interacting protein (CHIP) mediates poly-ubiquitylation preferentially on four-repeat Tau and is involved in neurodegeneration of tauopathy
    Shigetsugu Hatakeyama
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Fukuoka, Japan
    J Neurochem 91:299-307. 2004
    ..Here, we show that carboxyl terminus of Hsc70-interacting protein (CHIP), a U-box protein, recognizes the microtubule-binding repeat region of tau and preferentially ubiquitylates four-..
  33. pmc Notch-induced E2A degradation requires CHIP and Hsc70 as novel facilitators of ubiquitination
    Zhong Huang
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE 13th St, Oklahoma City, OK 73104, USA
    Mol Cell Biol 24:8951-62. 2004
    ..we conducted a yeast two-hybrid screen and identified the carboxyl terminus of Hsc70-interacting protein (CHIP) as an E47 binding protein...
  34. pmc Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly
    Michael S Y Huen
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Cell Biol 28:6104-12. 2008
    ..We propose that the RNF8 RING domain selects and loads a subset of UBC13 molecules, distinct from those that exist as heterodimers, onto sites of double-strand breaks, which facilitates the amplification of DNA damage signals...
  35. doi CHIP functions an E3 ubiquitin ligase of Runx1
    Yu Shang
    School of Medicine, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Biochem Biophys Res Commun 386:242-6. 2009
    ..Improper expression and mutations in Runx1 are frequently implicated in human leukemia. Here, we report that CHIP, the carboxyl terminus of Hsc70-interacting protein, also named Stub1, physically interacts with Runx1 through the ..
  36. pmc Co-chaperone CHIP stabilizes aggregate-prone malin, a ubiquitin ligase mutated in Lafora disease
    Sudheendra N R Rao
    Cellular and Molecular Neuroscience Laboratory, National Brain Research Centre, Manesar, Gurgaon 122 050, India
    J Biol Chem 285:1404-13. 2010
    ..Finally, we demonstrate that the co-chaperone carboxyl terminus of the Hsc70-interacting protein (CHIP) stabilizes malin by modulating the activity of Hsp70...
  37. pmc Hsp70 and CHIP selectively mediate ubiquitination and degradation of hypoxia-inducible factor (HIF)-1alpha but Not HIF-2alpha
    Weibo Luo
    From the Vascular Program, Institute for Cell Engineering
    J Biol Chem 285:3651-63. 2010
    ..Here, we identify Hsp70 and CHIP (carboxyl terminus of Hsc70-interacting protein) as HIF-1alpha-interacting proteins...
  38. pmc A caspase cleaved form of tau is preferentially degraded through the autophagy pathway
    Philip J Dolan
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 285:21978-87. 2010
    ....
  39. pmc CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response
    Takeshi Maruyama
    Department of Medical Pharmaceutics, Laboratory of Cell Signaling, Japan Science and Technology Corporation, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 29:2501-14. 2010
    ..Moreover, we identified the E3 ubiquitin ligase carboxyl terminus of Hsc70-interacting protein (CHIP) as a binding partner of MEKK2...
  40. pmc Redox regulation of the stability of the SUMO protease SENP3 via interactions with CHIP and Hsp90
    Shan Yan
    Department of Biochemistry and Cell Biology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, PR China
    EMBO J 29:3773-86. 2010
    ..heat shock protein 90 (Hsp90) and the co-chaperone/ubiquitin ligase carboxyl terminus of Hsc70-interacting protein (CHIP) control the turnover of client proteins...
  41. pmc CHIP promotes human telomerase reverse transcriptase degradation and negatively regulates telomerase activity
    Ji Hoon Lee
    Departments of Biology and Biomedical Science, World Class University Program of Graduate School, Yonsei University, Seoul 20 749, Korea
    J Biol Chem 285:42033-45. 2010
    ..Here, we show that CHIP (C terminus of Hsc70-interacting protein) physically associates with hTERT in the cytoplasm and regulates the ..
  42. pmc Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP
    K Matthew Scaglione
    Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 43:599-612. 2011
    ..Here we describe a series of molecular events that coordinately regulate CHIP, a neuroprotective E3 implicated in protein quality control...
  43. doi C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balances
    P Muller
    Masaryk Memorial Cancer Institute, Brno, Czech Republic
    Oncogene 32:3101-10. 2013
    ..The mechanisms regulating these opposite activities involve Hsp binding to co-chaperones including CHIP and HOP at their C-termini...
  44. doi Inhibition of histone deacetylase activity suppresses IFN-γ induction of tripartite motif 22 via CHIP-mediated proteasomal degradation of IRF-1
    Bo Gao
    Department of Immunology, Institute for Immunobiology, Shanghai Medical College of Fudan University, Shanghai 200032, People s Republic of China
    J Immunol 191:464-71. 2013
    ..This study may provide novel insight into the role of HDAC activity in the transcriptional control of IFN-stimulated gene induction...
  45. doi CHIP/Stub1 functions as a tumor suppressor and represses NF-κB-mediated signaling in colorectal cancer
    Yangmeng Wang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, National Engineering Laboratory for Anti tumor Therapeutics, Tsinghua University, Beijing 100084, China
    Carcinogenesis 35:983-91. 2014
    The carboxyl terminus of Hsc70-interacting protein (CHIP, also named Stub1), a U-box containing E3 ubiquitin ligase, is involved in degradation of certain oncogenic proteins...
  46. ncbi The co-chaperone CHIP regulates protein triage decisions mediated by heat-shock proteins
    P Connell
    Division of Cardiology, Galveston, Texas, University of Texas Medical Branch, Galveston, Texas, USA
    Nat Cell Biol 3:93-6. 2001
    ..We previously identified CHIP (carboxyl terminus of Hsc70-interacting protein) to be an interaction partner of Hsc70 (ref. 4)...
  47. ncbi The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation
    G C Meacham
    Department of Cell Biology and Cystic Fibrosis Center, University of Alabama Medical Center, 1918 University Boulevard, Birmingham, Alabama 35294 0005, USA
    Nat Cell Biol 3:100-5. 2001
    ..b>CHIP is a cytosolic U-box protein that interacts with Hsc70 through a set of tetratricorepeat motifs...
  48. pmc CHIP is a chaperone-dependent E3 ligase that ubiquitylates unfolded protein
    S Murata
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, and CREST, Japan Science and Technology Corporation JST, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    EMBO Rep 2:1133-8. 2001
    ..Here, we report that the C-terminus of Hsc70-interacting protein (CHIP) with a U-box domain is an E3 ubiquitin-ligase collaborating with molecular chaperones Hsp90 and Hsc70...
  49. ncbi ErbB2 degradation mediated by the co-chaperone protein CHIP
    Pengcheng Zhou
    Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:13829-37. 2003
    ..Here, we show that ErbB2 serves as an in vitro substrate for the Hsp70/Hsp90-associated U-box ubiquitin ligase CHIP. Overexpression of wild type CHIP, but not its U-box mutant H260Q, induced ubiquitinylation and reduction in both ..
  50. ncbi CHIP promotes proteasomal degradation of familial ALS-linked mutant SOD1 by ubiquitinating Hsp/Hsc70
    Makoto Urushitani
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute, Wako, Saitama, Japan
    J Neurochem 90:231-44. 2004
    ..heat-shock protein (Hsp) or heat-shock cognate (Hsc)70, and the carboxyl terminus of the Hsc70-interacting protein (CHIP), are involved in proteasomal degradation of mutant SOD1...
  51. ncbi Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase
    Hwei Ming Peng
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Biol Chem 279:52970-7. 2004
    ..In the current study, we have examined whether CHIP, a chaperone-dependent E3 ubiquitin-protein isopeptide ligase that is known to ubiquitylate other hsp90-chaperoned ..
  52. pmc Ligand-dependent switching of ubiquitin-proteasome pathways for estrogen receptor
    Yukiyo Tateishi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan
    EMBO J 23:4813-23. 2004
    ..unliganded ERalpha and identified a protein complex containing the carboxyl terminus of Hsc70-interacting protein (CHIP). CHIP preferentially bound to misfolded ERalpha and ubiquitinated it to induce degradation...
  53. ncbi CHIP controls the sensitivity of transforming growth factor-beta signaling by modulating the basal level of Smad3 through ubiquitin-mediated degradation
    Hong Xin
    Department of Biological Sciences and Biotechnology, and Institute of Biomedicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 280:20842-50. 2005
    ..Here, we report evidence for a novel mechanism of regulating TGF-beta sensitivity that depends on the role of CHIP (carboxyl terminus of Hsc70-interacting protein) in regulating the basal level of Smad3 via the ubiquitin-dependent ..
  54. ncbi The co-chaperone carboxyl terminus of Hsp70-interacting protein (CHIP) mediates alpha-synuclein degradation decisions between proteasomal and lysosomal pathways
    Youngah Shin
    Alzheimer Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:23727-34. 2005
    ..In the current study, we investigate the role of the carboxyl terminus of Hsp70-interacting protein (CHIP) in alpha-synuclein aggregation...
  55. ncbi HSJ1 is a neuronal shuttling factor for the sorting of chaperone clients to the proteasome
    Britta Westhoff
    Institute for Cell Biology, Rheinische Friedrich Wilhelms University Bonn, Ulrich Haberland Strasse 61a, D 53121 Bonn, Germany
    Curr Biol 15:1058-64. 2005
    ..In this way, HSJ1 isoforms participate in ER-associated degradation (ERAD) and protect neurons against cytotoxic protein aggregation...
  56. ncbi Regulation of the cytoplasmic quality control protein degradation pathway by BAG2
    Qian Dai
    Carolina Cardiovascular Biology Center and the Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599 7126, USA
    J Biol Chem 280:38673-81. 2005
    ..b>CHIP (carboxyl terminus of Hsp70-interacting protein) is an Hsp70-associated ubiquitin ligase that participates in this ..
  57. pmc BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP
    Verena Arndt
    Institute for Cell Biology, Rheinische Friedrich Wilhelms University Bonn, D 53121 Bonn, Germany
    Mol Biol Cell 16:5891-900. 2005
    ..The cochaperone CHIP is of central importance on this pathway, because it acts as a chaperone-associated ubiquitin ligase...
  58. pmc CHIP-mediated stress recovery by sequential ubiquitination of substrates and Hsp70
    Shu Bing Qian
    Carolina Cardiovascular Biology Center, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Nature 440:551-5. 2006
    ..b>CHIP (carboxy terminus of Hsp70-binding protein), a dual-function co-chaperone/ubiquitin ligase, targets a broad range ..
  59. ncbi A novel route for F-box protein-mediated ubiquitination links CHIP to glycoprotein quality control
    Rick F Nelson
    Medical Scientist Training Program, Graduate Program in Neuroscience, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 281:20242-51. 2006
    ..ubiquitination of glycoproteins discarded from the endoplasmic reticulum, binds the co-chaperone/ubiquitin ligase CHIP (C terminus of Hsc-70-interacting protein) through a unique N-terminal PEST domain in Fbx2...
  60. ncbi CHIP protects from the neurotoxicity of expanded and wild-type ataxin-1 and promotes their ubiquitination and degradation
    Ismael Al-Ramahi
    Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:26714-24. 2006
    b>CHIP (C terminus of Hsc-70 interacting protein) is an E3 ligase that links the protein folding machinery with the ubiquitin-proteasome system and has been implicated in disorders characterized by protein misfolding and aggregation...
  61. ncbi Dorfin-CHIP chimeric proteins potently ubiquitylate and degrade familial ALS-related mutant SOD1 proteins and reduce their cellular toxicity
    Shinsuke Ishigaki
    Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya 466 8500, Japan
    Neurobiol Dis 25:331-41. 2007
    ..hydrophobic portion of Dorfin and the U-box domain of the carboxyl terminus of Hsc70-interacting protein (CHIP), which has strong E3 activity through the U-box domain...
  62. ncbi Curcumin-induced degradation of ErbB2: A role for the E3 ubiquitin ligase CHIP and the Michael reaction acceptor activity of curcumin
    YunJin Jung
    The Research Institute for Drug Development, College of Pharmacy, Pusan National University, Busan, 609 735, Korea
    Biochim Biophys Acta 1773:383-90. 2007
    ..Curcumin increased association of endogenous and ectopically expressed CHIP, a chaperone-dependent ubiquitin ligase, with ErbB2...
  63. pmc The high-affinity HSP90-CHIP complex recognizes and selectively degrades phosphorylated tau client proteins
    Chad A Dickey
    Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida, USA
    J Clin Invest 117:648-58. 2007
    ..A critical mediator of this mechanism was carboxy terminus of Hsp70-interacting protein (CHIP), a tau ubiquitin ligase...
  64. ncbi Certain pairs of ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s) synthesize nondegradable forked ubiquitin chains containing all possible isopeptide linkages
    Hyoung Tae Kim
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:17375-86. 2007
    ..purified E3s and Ub-conjugating enzymes (E2s), we found, using Ub mutants and mass spectrometry, that the U-box E3, CHIP, and Ring finger E3s, MuRF1 and Mdm2, with the E2, UbcH5, form a novel type of Ub chain that contains all seven ..
  65. ncbi Chaperone functions of the E3 ubiquitin ligase CHIP
    Meredith F N Rosser
    Department of Cell and Developmental Biology, University of North Carolina Chapel Hill School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:22267-77. 2007
    The carboxyl terminus of the Hsc70-interacting protein (CHIP) is an Hsp70 co-chaperone as well as an E3 ubiquitin ligase that protects cells from proteotoxic stress...
  66. ncbi Chaperone-dependent E3 ligase CHIP ubiquitinates and mediates proteasomal degradation of soluble guanylyl cyclase
    Tian Xia
    Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912 2500, USA
    Am J Physiol Heart Circ Physiol 293:H3080-7. 2007
    ..In this study, we have investigated whether COOH terminus of heat shock protein 70-interacting protein (CHIP), a co-chaperone molecule that is involved in protein folding but is also a chaperone-dependent ubiquitin E3 ligase,..
  67. ncbi Differential ubiquitination of Smad1 mediated by CHIP: implications in the regulation of the bone morphogenetic protein signaling pathway
    Ren Feng Li
    Department of Biological Sciences and Biotechnology, State Key Lab of Biomembranes and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    J Mol Biol 374:777-90. 2007
    ..and K269) of Smad1 are ubiquitin acceptor sites mediated by the carboxyl terminus of Hsc70-interacting protein (CHIP)...
  68. ncbi Two different classes of E2 ubiquitin-conjugating enzymes are required for the mono-ubiquitination of proteins and elongation by polyubiquitin chains with a specific topology
    Mark Windheim
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 409:723-9. 2008
    ..For this reason, chain topology must be tightly controlled. Using the U-box E3 ligase CHIP [C-terminus of the Hsc (heat-shock cognate) 70-interacting protein] and the RING E3 ligase TRAF6 (tumour-necrosis-..
  69. doi CHIP-mediated degradation and DNA damage-dependent stabilization regulate base excision repair proteins
    Jason L Parsons
    Medical Research Council Radiation Oncology and Biology Unit, University of Oxford, Oxfordshire OX1 3QU, UK
    Mol Cell 29:477-87. 2008
    ..on damaged DNA and that proteins not involved in a repair complex are ubiquitylated by the E3 ubiquitin ligase CHIP and subsequently rapidly degraded...
  70. pmc CHIP deficiency decreases longevity, with accelerated aging phenotypes accompanied by altered protein quality control
    Jin Na Min
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, North Carolina 27599 7126, USA
    Mol Cell Biol 28:4018-25. 2008
    ..The carboxyl terminus of Hsp70-interacting protein (CHIP), a ubiquitin ligase/cochaperone, participates in protein quality control by targeting a broad range of chaperone ..
  71. pmc CHIP targets toxic alpha-Synuclein oligomers for degradation
    Julie E Tetzlaff
    Department of Neurology, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Charlestown, MA 02129, USA
    J Biol Chem 283:17962-8. 2008
    ..Here, we studied the effect of a potent molecular chaperone, CHIP (carboxyl terminus of Hsp70-interacting protein), on alphaSyn oligomerization using a novel bimolecular ..
  72. pmc CHIP deletion reveals functional redundancy of E3 ligases in promoting degradation of both signaling proteins and expanded glutamine proteins
    Yoshihiro Morishima
    Department of Pharmacology, The University of Michigan Medical School, 3510 MSRB I, 1150 W Medical Center Dr, Ann Arbor, MI 48109, USA
    Hum Mol Genet 17:3942-52. 2008
    b>CHIP (carboxy terminus of Hsc70-interacting protein) an E3 ubiquitin ligase that binds to Hsp70 and Hsp90, promotes degradation of several Hsp90-regulated signaling proteins and disease-causing proteins containing expanded glutamine ..
  73. pmc A critical role for CHIP in the aggresome pathway
    Youbao Sha
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 29:116-28. 2009
    ..b>CHIP (carboxy terminus of Hsp70-interacting protein) is a highly conserved protein that has been shown to mediate ..
  74. pmc CHIP regulates leucine-rich repeat kinase-2 ubiquitination, degradation, and toxicity
    Han Seok Ko
    Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:2897-902. 2009
    ..Here we show that the carboxyl terminus of HSP70-interacting protein (CHIP) binds, ubiquitinates, and promotes the ubiquitin proteasomal degradation of LRRK2...
  75. doi The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways
    Masashi Kajiro
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Nat Cell Biol 11:312-9. 2009
    b>CHIP is a U-box-type ubiquitin ligase that induces ubiquitylation and degradation of its substrates, which include several oncogenic proteins. The relationship between CHIP and tumour progression, however, has not been elucidated...
  76. pmc Regulation of LRRK2 stability by the E3 ubiquitin ligase CHIP
    Xiaodong Ding
    Department of Neurology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS ONE 4:e5949. 2009
    ..Here we show that the E3 ubiquitin ligase CHIP physically associates with LRRK2 and regulates the cellular abundance of LRRK2...
  77. pmc Identification of residues on Hsp70 and Hsp90 ubiquitinated by the cochaperone CHIP
    Lenka Kundrat
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Mol Biol 395:587-94. 2010
    ..The ubiquitin ligase C-terminus of Hsc70 interacting protein (CHIP) has been shown in vitro and in vivo to associate with Hsp70 and Hsp90 and ubiquitinate them, thus targeting them ..
  78. doi Promotion of CHIP-mediated p53 degradation protects the heart from ischemic injury
    Atsuhiko T Naito
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba, Japan
    Circ Res 106:1692-702. 2010
    ..Tumor suppressor protein p53 accumulates in the myocardium after myocardial infarction, causes apoptosis of cardiomyocytes, and plays an important role in the progression into heart failure...
  79. pmc Balance between folding and degradation for Hsp90-dependent client proteins: a key role for CHIP
    Lenka Kundrat
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Biochemistry 49:7428-38. 2010
    ..The central players are the molecular chaperones Hsp70 and Hsp90, the cochaperone HOP, and ubiquitin ligase, CHIP. Hsp70 and Hsp90 bind to HOP, thus forming a ternary folding complex whereas the binding of CHIP to the chaperones ..
  80. pmc Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP
    Vikram Narayan
    CRUK Interferon and Cell Signalling Group, Cell Signalling Unit, Institute of Genetics and Molecular Medicine, Crewe Road South, University of Edinburgh, Edinburgh EH4 2XR, United Kingdom
    J Biol Chem 286:607-19. 2011
    ..Whereas CHIP (C terminus of Hsc70-interacting protein), appears to chaperone IRF-1 in unstressed cells, formation of a stable ..
  81. doi CHIP-dependent p53 regulation occurs specifically during cellular senescence
    Christina Sisoula
    National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, Athens 11635, Greece
    Free Radic Biol Med 50:157-65. 2011
    ..However, other E3 ligases have been identified, such as the chaperone-associated ligase CHIP, although their precise function regarding p53 degradation remains elusive...
  82. pmc E3 ligase STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer disease patients
    Peter Tsvetkov
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 286:8839-45. 2011
    ..We show here that NQO1 protein level is regulated by the E3 ligase STUB1/CHIP (C terminus of Hsc70-interacting protein)...
  83. doi Gambogic acid-induced degradation of mutant p53 is mediated by proteasome and related to CHIP
    Jia Wang
    Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Jiangsu, China
    J Cell Biochem 112:509-19. 2011
    ..p53 was ubiquitinated and it was chaperones related ubiquitin ligase carboxy terminus of Hsp70-interacting protein (CHIP) rather than MDM2 involved in the degradation of mutant p53...
  84. doi Carboxyl terminus of Hsp70-interacting protein (CHIP) contributes to human glioma oncogenesis
    Tao Xu
    Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, China
    Cancer Sci 102:959-66. 2011
    ..Growing evidence has shown that E3 ubiquitin ligases can promote tumorgenesis of glioma. CHIP is an E3 ubiquitin ligase that can induce ubiquitylation and degradation of many tumor-related proteins, and it has ..
  85. pmc Control of AIF-mediated cell death by antagonistic functions of CHIP ubiquitin E3 ligase and USP2 deubiquitinating enzyme
    K H Oh
    School of Biological Sciences, Seoul National University, Seoul, Korea
    Cell Death Differ 18:1326-36. 2011
    ..Here, we demonstrated that USP2 and CHIP contribute to the control of tAIF stability...
  86. doi The C-terminus of Hsp70-interacting protein promotes Met receptor degradation
    Kang Won Jang
    Infection Signaling Network Research Center, Chungnam National University, Daejeon, South Korea
    J Thorac Oncol 6:679-87. 2011
    ..Previous studies have shown that Met receptor levels are governed in part by Cbl-mediated ubiquitination and degradation, and the uncoupling of Met from this pathway promotes its transforming activity...
  87. pmc Molecular mechanism of the negative regulation of Smad1/5 protein by carboxyl terminus of Hsc70-interacting protein (CHIP)
    Le Wang
    MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China
    J Biol Chem 286:15883-94. 2011
    ..The C terminus of Hsc70-interacting protein (CHIP) serves as an E3 ubiquitin ligase to mediate the degradation of Smad proteins and many other signaling proteins...
  88. pmc Functional inactivation of endogenous MDM2 and CHIP by HSP90 causes aberrant stabilization of mutant p53 in human cancer cells
    Dun Li
    Department of Pathology, Stony Brook University, Stony Brook, NY 11794 8691, USA
    Mol Cancer Res 9:577-88. 2011
    ..p53 cancer cells, we show that in mutant p53 cancer cells, this HSP90 interaction blocks the endogenous MDM2 and CHIP (carboxy-terminus of Hsp70-interacting protein) E3 ligase activity...
  89. pmc E2 conjugating enzyme selectivity and requirements for function of the E3 ubiquitin ligase CHIP
    Sarah E Soss
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 286:21277-86. 2011
    ..b>CHIP (carboxyl terminus of Hsc70-interacting protein) is a U-box E3 ligase that serves as a co-chaperone to heat shock ..
  90. doi Ubiquitin ligase CHIP induces TRAF2 proteasomal degradation and NF-κB inactivation to regulate breast cancer cell invasion
    Kang Won Jang
    Graduate Program in Science for Aging and Yonsei Research Institute of Aging Science, Yonsei University, Seoul 120 749, Korea
    J Cell Biochem 112:3612-20. 2011
    ..The carboxyl terminus of Hsc70-interacting protein (CHIP) is a U-box-type ubiquitin ligase that induces ubiquitination and proteasomal degradation of its substrate proteins...
  91. doi miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression
    Junwei Guo
    State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Tsinghua University, Beijing, China
    J Bone Miner Res 27:1607-18. 2012
    ..We previously reported that C terminus of Hsc70-interacting protein/STIP1 homology and U-Box containing protein 1 (CHIP/STUB1) negatively regulated osteoblast differentiation through promoting Runx2 protein degradation...
  92. pmc The chaperone-assisted E3 ligase C terminus of Hsc70-interacting protein (CHIP) targets PTEN for proteasomal degradation
    Syed Feroj Ahmed
    Signal Tranduction in Cancer and Stem Cells Laboratory, Council of Scientific and Industrial Research CSIR Indian Institute of Chemical Biology IICB, 4 Raja S C Mullick Road, Kolkata, 700032, West Bengal, India
    J Biol Chem 287:15996-6006. 2012
    ..Nedd 4-1, XIAP, and WWP2 have been shown to maintain PTEN turnover. Here, we report that CHIP, the chaperone-associated E3 ligase, induces ubiquitination and regulates the proteasomal turnover of PTEN...
  93. doi CHIP functions as a novel suppressor of tumour angiogenesis with prognostic significance in human gastric cancer
    Shouyu Wang
    Department of Molecular Cell Biology and Toxicology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Centre, School of Public Health, Nanjing Medical University, Nanjing 210029, People s Republic of China
    Gut 62:496-508. 2013
    b>CHIP (carboxy terminus of Hsc70 interacting protein) is an E3 ubiquitin ligase that can induce ubiquitination and degradation of several tumour related proteins, and acts as a suppressor of tumour metastasis...
  94. doi The ubiquitin ligase CHIP regulates c-Myc stability and transcriptional activity
    I Paul
    Laboratory of Signal transduction in cancer and Stem cells, Indian Institute of Chemical Biology IICB, Council of Scientific and Industrial Research CSIR, Kolkata, India
    Oncogene 32:1284-95. 2013
    ..Here, we report a new E3 ligase for c-Myc, the carboxyl terminus of Hsc70-interacting protein or CHIP, which is a chaperone-associated Ubox-containing E3 ligase...
  95. doi Alterations of the Hsp70/Hsp90 chaperone and the HOP/CHIP co-chaperone system in cancer
    Eva Růcková
    Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, 656 53, Czech Republic
    Cell Mol Biol Lett 17:446-58. 2012
    ..cochaperones; therefore we investigated the expression of Hsp70 and Hsp90 and their specific co-chaperones HOP and CHIP in cancer cell lines and primary cancers...
  96. pmc Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis
    Anna Plechanovova
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nature 489:115-20. 2012
    ..This arrangement is primed for catalysis as it can deprotonate the incoming substrate lysine residue and stabilize the consequent tetrahedral transition-state intermediate...
  97. pmc Androgen receptor degradation by the E3 ligase CHIP modulates mitotic arrest in prostate cancer cells
    S Sarkar
    Department of Radiation Oncology, University of Virginia Health Sciences Center, University of Virginia School of Medicine, Charlottesville, VA, USA
    Oncogene 33:26-33. 2014
    ..estrogen metabolite, induces mitotic arrest in prostate cancer cells involving activation of the E3 ligase CHIP (C-terminus of Hsp70-interacting protein) and degradation of the AR...
  98. pmc Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein)
    Seiko Shimamoto
    Department of Signal Transduction Sciences, Kagawa University Faculty of Medicine, 1750 1 Ikenobe, Miki cho, Kita gun, Kagawa, Japan
    J Biol Chem 288:7158-68. 2013
    The U-box E3 ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein) binds Hsp90 and/or Hsp70 via its tetratricopeptide repeat (TPR), facilitating ubiquitination of the chaperone-bound client proteins...
  99. doi Metastatic lymph node CHIP expression is a potential prognostic marker for resected esophageal squamous cell carcinoma patients
    Jing Wen
    State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat Sen University, Guangzhou, People s Republic of China
    Ann Surg Oncol 20:1668-75. 2013
    C-terminal Hsp-interacting protein (CHIP) is an HSP70 and HSP90 interacting co-chaperone and an E3 ubiquitin ligase. Previous studies have reported the role of CHIP in cancer progression by targeting protein degradation...
  100. pmc The ubiquitin ligase Stub1 negatively modulates regulatory T cell suppressive activity by promoting degradation of the transcription factor Foxp3
    Zuojia Chen
    Key Laboratory of Molecular Virology and Immunology, Unit of Molecular Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China
    Immunity 39:272-85. 2013
    ....
  101. pmc Endoplasmic reticulum protein quality control is determined by cooperative interactions between Hsp/c70 protein and the CHIP E3 ligase
    Yoshihiro Matsumura
    From the Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239 and
    J Biol Chem 288:31069-79. 2013
    The C terminus of Hsp70 interacting protein (CHIP) E3 ligase functions as a key regulator of protein quality control by binding the C-terminal (M/I)EEVD peptide motif of Hsp/c70(90) with its N-terminal tetratricopeptide repeat (TPR) ..

Research Grants95

  1. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  2. Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development
    JOSE MANUEL MORACHIS; Fiscal Year: 2010
    ..This proposed project combines cutting edge lab-on-a-chip technology (microfluidics, optics, microacoustics) with expertise in research applications in cancer and other ..
  3. Materials and Strategies for Lab-on-a-Chip
    Sonia Grego; Fiscal Year: 2007
    ..by applicant): [unreadable] The goal of this symposium is to discuss material and engineering aspects of lab-on-a-chip devices and the application of microfluidics to materials synthesis...
  4. Simulation Tool to Rapidly Design Optimize and Prototype Microfluidic Devices
    Yi Wang; Fiscal Year: 2010
    ..Current chip design methods rely primarily on trial-and-error experiments or employ prohibitively expensive high-fidelity ..
  5. Integrated Microfluidic Devices for 18F Labeled PET Probes in Cancer Imaging
    Hsian Rong Tseng; Fiscal Year: 2010
    ..Initially, we will develop three separate microfluidic functional modules in parallel, including: (i) An off-chip module for concentration of [18F]fluoride, (ii) the core-a set of digitally controlled microfluidic circuits for ..
  6. Passive Flow Control for Integrated Genomic Analysis Microchips
    MATTHEW RICHARD BEGLEY; Fiscal Year: 2013
    ..to develop a passive flow control paradigm for microfluidic devices, which will reduce the need for external (off-chip) hardware and facilitate the development of widely distributable hand-held bioanalytical devices...
  7. Point Detection of Pathogens in Oral Samples via UPT
    Daniel Malamud; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): We are developing a portable, credit-card sized microfluidic lab-on-a-chip for rapid point-of-care detection of multiple bacterial and viral targets using oral fluids...
  8. Microchip to Detect Influenza Infection and Type in Nasopharyngeal Swabs
    Catherine M Klapperich; Fiscal Year: 2010
    ..We have developed a plastic lab-on-a-chip platform for sample preparation, amplification and detection of viral RNA in human samples using microfluidic ..
  9. High-Throughput Plastic Microfabrication Technologies for Smart Lab-on-a-Chips
    ANIRUDDHA PUNTAMBEKAR; Fiscal Year: 2009
    ..effort is the development of reliable, high-throughput microfabrication techniques for production of lab-on-a-chip for Point-of-Care Testing (POCT) applications...
  10. Xiujun Li; Fiscal Year: 2015
    ..capture zones on microfluidic devices allow for the detection of these three different species of bacteria on chip simultaneously and specifically, based on DNA hybridization with specific DNA capture probes...
  11. Neurovascular Unit on a Chip: Chemical Communication, Drug and Toxin Responses
    JOHN PETER WIKSWO; Fiscal Year: 2013
    ..An integrated approach will provide technologies of widespread applicability and reveal new mechanistic and region-specific insights into how the brain receives, modifies, and is affected by drugs, neurotropic agents and disease. ..
  12. Steven S An; Fiscal Year: 2015
    ..structural analysis, we will implement the high-throughput custom-built microfluidic devices that allow the in- chip design for culturing live cells...
  13. Disposable Microfluidic Devices for Point of Care Diagnostics
    Catherine M Klapperich; Fiscal Year: 2010
    ..The chip described here will be able to perform these tasks in a matter of minutes to hours as opposed to days...
  14. Erwin Berthier; Fiscal Year: 2014
    ..Lastly, a wax-based sealing mechanism will be integrated into the SLIDE to enable on-chip reverse transcription, which stabilizes viral RNA into DNA, and sealing for transport...
  15. A Microfluidic Device for Label-free HIV Virion Count at Point of Care Settings
    Xuanhong Cheng; Fiscal Year: 2010
    ..In Aim 2, we also plan to integrate all the viral processing components from Aim 1 into a single microfluidic chip and test the collective performance of these components interfaced with the pressure sensor using virus-containing ..
  16. ADAM THOMAS WOOLLEY; Fiscal Year: 2016
    ..Aim 2 will involve developing PTB biomarker analysis microdevices that use pressure actuation of on-chip valves and pumps to carry out integrated sample preparation operations...
  17. Integrated Platform for In Vitro Culturing of Cells and Tissues
    Colleen K Van Pelt; Fiscal Year: 2013
    ..platform will offer an interconnect solution with the potential of becoming the standard for "cells-on-a-chip" applications...
  18. Microchips of Protein Kinase Substrates
    XIAOCHUAN contact ZHOU; Fiscal Year: 2010
    ..a robust, quantitative, comprehensive, flexible, high throughput, cost effective and scalable PK profiling peptide chip platform...
  19. Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures
    Aydogan Ozcan; Fiscal Year: 2009
    ..a set of applications that would highly benefit from miniaturized high resolution microscopes that are ideally on chip. For this purpose, the proposed research plan aims to develop a new form of lens-free, compact digital microscope ..
  20. Microbead INtegrated DNA Sequencer (MINDS) System
    Stevan Jovanovich; Fiscal Year: 2007
    ..produce fluorescently labeled sequencing fragments that are efficiently captured concentrated and purified using on-chip affinity capture. The fragments are then separated and sized on a proven microfabricated uCAE sequencer...
  21. RONALD JAY KOENIG; Fiscal Year: 2014
    ..In the first Specific Aim, chromatin immunoprecipitation - deep sequencing (ChIP-Seq) will be used to identify PPFP binding sites on a genome wide level...
  22. SATB1-dependent epigenetic regulation of leukemia initiating cells
    Britta Will; Fiscal Year: 2011
    ..We will utilize global chromatin immunoprecipitation analysis (ChIP-seq) in combination with gene expression and DNA methylation pattern analysis...
  23. Vasiliki Poulaki; Fiscal Year: 2016
    ..analysis after siRNA- mediated silencing of these transcription factors, and chromatin immunoprecipitation (ChIP)- Sequencing (ChIP-Seq) analysis, we will determine their transcriptional target genes...
  24. Rugang Zhang; Fiscal Year: 2016
    ..We performed whole-genome chromatin immunoprecipitation (ChIP) followed by next generation sequencing analysis and gene expression profiling...
  25. Analysis of genome wide transcriptional control in yeast
    Vishwanath R Iyer; Fiscal Year: 2013
    ..responses that they are shown to be required for, using chromatin immunoprecipitation combined with microarrays (ChIP-chip)...
  26. A Center for Zebrafish Chromatin and Epigenetics
    David A Jones; Fiscal Year: 2012
    ..We will combine chromatin immunoprecipitation (ChIP) with high-density genome tiling arrays (to examine chromatin), and also perform gene expression profiling...
  27. Dynamic Usage of Network Motifs in Retinal Development and Diseases
    Jiang Qian; Fiscal Year: 2012
    ..we will integrate several large-scale datasets, including chromatin immunoprecipitation coupled with microarray (ChIP-chip) and with sequencing (ChIP-seq) and gene expression profiling in different conditions...
  28. Rulang Jiang; Fiscal Year: 2014
    ..Recent advances in massively parallel sequencing technologies, in combination with chromatin immunoprecipitation (ChIP-seq) and gene expression profiling approaches, have enabled comprehensive analyses of genome-wide transcriptional ..
  29. Multiplexed Onchip Nanomagnetic Immunoassay for TOxin Reporting (MONITOR)
    John Collins; Fiscal Year: 2012
    ..Onchip Nanomagnetic Immunoassay for Toxin Reporting (MONITOR) Micro/Nano engineering techniques such as lab-on-a-chip technologies and nanoparticulates sensors are emerging as the next revolution tools for integrated high sensitive ..
  30. HIGH SENSITIVITY, ULTRA-LOW BACKGROUND DNA CHIPS
    Mary Anne Watt; Fiscal Year: 2002
    ..The ideal DNA chip must be easy to use, allow high density target DNA attachment, display extremely high signal to noise ratios, be ..
  31. Yun Fai Chris Lau; Fiscal Year: 2014
    ..of a TSPY-AR transcription complex using advanced chromatin immunoprecipitation and promoter tiling microarray (ChIP-Chip) approach...
  32. Integrated Microfluidic Platform for Point-of-Care Serodiagnostics
    Xiaowu Liang; Fiscal Year: 2013
    ..Lee) to develop a point-of-care diagnostic system consisting of a cost-effective disposable microfluidic plastic chip and a portable analyzer that can be driven by a mobile computing device for rapid serological evaluation of ..
  33. A Nanofluidic Nanoplasmonic Platform for Multiplexing Detection of Cancer Biomark
    Jianjun Wei; Fiscal Year: 2010
    ..interfaces with microfluidic channels, making it amenable to highly parallel throughput screening in a lab-on-chip device...
  34. Vitamin D Hormone Signaling in Bone Mineral Homeostasis
    Mark R Haussler; Fiscal Year: 2010
    ..Methodology will include genomics and chromatin immunoprecipitation (ChIP) assays to scan for candidate VDREs, plus DNA gel mobility shift, real time PCR and promoter dissection to identify ..
  35. Balakuntalam S Kasinath; Fiscal Year: 2016
    ..Chromatin immunoprecipitation (ChIP) assays will be performed to study GSK 3beta regulation of binding of Groucho, TCF/LEF1 to the fibronectin promoter...
  36. Regulation of Airway Mucin Gene Expression by Epigenetic Mechanism
    Reen Wu; Fiscal Year: 2013
    ..Chromatin immunoprecipitation (ChIP) approaches with anti-methylated deoxy-cytosine and various anti-modified histone antibodies will be used to assess ..
  37. Regulation of the Vibrio cholerae intestinal exit plan
    ANISIA J SILVA BENITEZ; Fiscal Year: 2010
    ..this hypothesis by integrating a variety of approaches such as bacterial genetics, chromatin immunoprecipitation (ChIP), ChiP-sequencing, confocal microscopy and animal models...
  38. John W Wiley; Fiscal Year: 2016
    ..regulatory CpG methylation sites at the promoters of stress response genes and chromatin immunoprecipitation (ChIP) analysis of relevant histone modification targets...
  39. Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate Cancer
    BARRY MATTHEW MARKAVERICH; Fiscal Year: 2013
    ..Chromatin immunoprecipitation (ChIP) studies revealed that L reduced the acetylation state of histone H4 (at Lys 5, 8, 12, 16) associated with the PLK1 ..
  40. ROBERT MICHAEL VAN DAM; Fiscal Year: 2015
    ..This proof-of-concept chip will be further developed in this application into a robust, automated, compact system for routinely concentrating ..
  41. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..Techniques to be utilized include chromatin immunoprecipitation experiments followed by array hybridization (ChIP-chip) or whole genome sequencing (ChIP-seq), high throughput DNase I hypersensitive site mapping, and functional ..
  42. Epigenetic Analysis of iPS Cell Models of Aging and Alzheimer's Disease
    Bruce A Yankner; Fiscal Year: 2010
    ..be performed using microarrays and by applying recent innovations in deep sequencing chromatin immunoprecipitation (ChIP- seq)...
  43. Cellular and Molecular Regulation of Thrombopoiesis
    Ramesh A Shivdasani; Fiscal Year: 2012
    ..We have combined chromatin immunoprecipitation with hybridization to tiled genome arrays (ChIP-chip) to identify the complete complement of genes that NF-E2 may regulate in MKs...
  44. Regulation of Bile Acid Synthesis by Nuclear Receptors
    John Y L Chiang; Fiscal Year: 2010
    ..HepG2 and primary human hepatocytes will be used for chromatin immunoprecipitation (ChIP), co-IP and mammalian two-hybrid assays to study DNA-protein and protein-protein interaction...
  45. Peter B Dervan; Fiscal Year: 2014
    ..scale;most notably through the coupling of chromatin immunoprecipitation to massively parallel sequencing (ChIP-Seq)...
  46. Mechanisms of Early Growth Response Factor 1 (Egr-1) Induction by HSV-1 Lytic Inf
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2010
    ..Chromatin Immunoprecipitation (ChIP) assays demonstrated that NFkB and cAMP response element binding protein (CREB) was induced by HSV-1 infection and ..
  47. Paul M Lieberman; Fiscal Year: 2016
    ..with the host-chromosome, we used chromatin immunoprecipitation combined with next generation DNA sequencing (ChIP-Seq) and functional genomic methods to identify and characterize EBNA1 binding sites in the human genome...
  48. Erythropoietin in Non-Erythroid Cells: Function and Regulation
    Josef T Prchal; Fiscal Year: 2013
    ..We have studied the mechanism of HIF regulation of Epo transcription using chromatin immunoprecipitation (ChIP) and have found multiple HREs of the Epo gene...
  49. Michel G Bergeron; Fiscal Year: 2014
    ..their already existing diagnostic technologies into a single Microfluidic Centripetal Device (MCD), or Lab-on-a-Chip, for the detection of VRTIs (VRTI-MCD)...
  50. AKAP Regulation of PKA Targeting in the Heart
    Meredith Bond; Fiscal Year: 2009
    ..In (new) Specific Aim 4.1, we will use Chromatin Immunoprecipitation (ChIP) followed by high throughput sequencing (ChIP-seq) to: (i) identify genes whose upstream regulatory sequence binds ..
  51. Arsenic, Histone Modification, and Transcription
    LYNN SHELDON; Fiscal Year: 2009
    ..virus (MMTV) promoter in the presence and absence of sodium arsenite in vivo by chromatin immunoprecipitation (ChIP) analysis (Specific Aim I)...
  52. Instacortisol: A realtime and continuous assessment of cortisol in ISF
    MANJU GROVER VENUGOPAL; Fiscal Year: 2012
    ..A virtual evaluation of cortisol sensor chip and its integration into a compact easy to use design will be carried out in parallel...
  53. A Cis-regulatory Model for Neural Border Induction
    Daniel Meulemans Medeiros; Fiscal Year: 2013
    ..The proposed work will test this model using mRNA injections, mutagenesis, and chromatin immunoprecipitation (ChIP)...
  54. Excessive Copper Levels Disrupt Hepatic Nuclear Receptor Function
    Clavia Ruth Wooton-Kee; Fiscal Year: 2011
    ..utilize the Atp7b-/- mouse to determine nuclear receptor DNA-binding activity via chromatin immunoprecipitation (ChIP) assays and to measure parameters of liver function, such as bile flow, biliary secretion of copper, bile acids, ..
  55. Peter L Freddolino; Fiscal Year: 2014
    ..IPODHR is superficially similar to chromatin immunoprecipitation (ChIP) experiments, but instead of isolating a single protein (and any DNA bound to it), IPODHR isolates all protein-DNA ..
  56. PROMOTER SPECIFICITY OF TRANSCRIPTION FACTORS
    David J Stillman; Fiscal Year: 2010
    ..Chromatin immunoprecipitation (ChIP) experiments show that there are two distinct mechanisms operative to prevent these factors from activating genes...
  57. Maha Abdellatif; Fiscal Year: 2016
    ..More significantly, chromatin immunoprecipitation-deep sequencing analysis (ChIP-Seq) reveals that supplementing cardiac myocytes with miR-1 suppresses de novo pol II recruitment on a subset of ..
  58. Ultraminiaturized microfluidics-based drug toxicity screening platform using iPSC
    Zhizhong Yin; Fiscal Year: 2013
    ..proof-of-principle that iPSC-derived hepatocytes maintain their viability and functionality in our microfluidic chip and display relevant drug responses...
  59. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..Second, we will perform ChIP and quantitative PCR on chromatin from lymphoid cells to determine if there are locus, stage or lineage-specific ..
  60. Amit Berson; Fiscal Year: 2015
    ..Second, using a recently developed in- vivo, cell type-specific chromatin immunoprecipitation and deep-sequencing (ChIP-Seq) technique, chromatin landscape alterations induced by TDP-43 will be characterized...
  61. Genomic actions of progesterone receptors in human myometrial cells
    Sam Mesiano; Fiscal Year: 2010
    ..to which PR- A and PR-B bind in hTERT-HMA/B cells will be identified by nPR-chromatin immunoprecipitation (ChIP) followed by microarray-based genome-wide DNA location analysis (ChIP-on-chip)...
  62. DEVELOPMENT OF SPLIT DAMID AS AN ALTERNATIVE METHODOLOGY TO CHROMATIN IMMUNOPRECI
    Raphael Kopan; Fiscal Year: 2010
    ..since the traditional methods used to identify the immediate targets of a transcription factor (chromatin IP or ChIP) are expensive, labor-intensive and technically demanding...
  63. Bogi Andersen; Fiscal Year: 2016
    ..We will use chromatin immunoprecipitation sequencing (ChIP-seq) and chromosome conformation capture (3C) assays to test the hypothesis that BMAL1 directly and indirectly ..
  64. Liang Tang; Fiscal Year: 2016
    ..metal oxide semiconductor) image sensor and nanohole array to develop an innovative, fully operational lab-on-a-chip biosensor...
  65. Epigenetic programming of brain development by choline nutrition
    Jan Krzysztof Blusztajn; Fiscal Year: 2011
    ..methylated DNA immunoprecipitation (MeDIP) followed by microarray analysis and 3) chromatin immunoprecipitation (ChIP) with antibodies against different methylated epitopes on specific lysine residues of histone 3 (e.g...
  66. RAYMOND EDWARD SOCCIO; Fiscal Year: 2016
    ..Our initial studies used chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq) to identify the genome-wide binding sites, or cistrome, for PPAR in cultured human adipocytes...
  67. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  68. SUSAN KANDARIAN; Fiscal Year: 2015
    ..This goal will entail measurement of global gene expression in conjunction with chromatin immunoprecipitation (ChIP) using transcription factor antibodies followed by massively parallel sequencing of the immunoprecipitated DNA ..
  69. Ramesh A Shivdasani; Fiscal Year: 2014
    ..We have used whole-genome chromatin immunoprecipitation (ChIP) to identify, with high confidence, regions of CDX2 occupancy in colonic epithelial cells...
  70. JORG BUNGERT; Fiscal Year: 2016
    ..will be characterized using a novel in vitro assembly assay as well as using chromatin immunoprecipitation (ChIP) in intact cells (Specific Aim 2)...
  71. The Role of the Cochaperone/E3 Ubiquitin Ligase CHIP in Nuclear Stress Response
    SARAH MARIE RONNEBAUM; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): CHIP is a protein with cochaperone and E3 ubiquitin ligase activities that mediates the cellular stress response by directing misfolded proteins for refolding or degradation...
  72. Molecular analysis of bipolar and schizophrenia candidate genes
    Herbert M Lachman; Fiscal Year: 2012
    ..we successfully implemented in the initial grant period was to use a chromatin immunoprecipitation based method - ChIP-chip - as a screening tool to identify novel regulatory elements in SZ and BD candidate genes...
  73. High-Throughput Low Cost Production of Chromatin-Immunoprecipitation-Grade Antib
    KEITH ELLIOTT KROPP; Fiscal Year: 2010
    ..Chromatin immunoprecipitation (ChIP) coupled with microarray (ChIP-chip) or sequencing (ChIP- seq) is a widely used technique for understanding the ..
  74. GATA Factor Regulation of Vascular Endothelium
    EMERY BRESNICK; Fiscal Year: 2009
    ..Chromatin immunoprecipitation (ChIP) and ChIP coupled to microarray chip (ChIP-chip) assays will be conducted to determine which GATA factor(s) occupy ..
  75. Genome-wide epigenetic control of circadian metabolism by heme receptor Rev-erb
    Mitchell A Lazar; Fiscal Year: 2010
    ..We will test this by genome-wide location analysis using chromatin immunoprecipitation (ChIP) followed by sequencing of the isolated DNA (ChIP-seq)...
  76. Hybridization of Promoter DNA Targets in Chromatin to Discover Regulatory Protein
    LINDA B MC GOWN; Fiscal Year: 2010
    Chromatin immunoprecipitation (ChIP) is a powerful tool for investigating the mechanisms through which nuclear proteins influence gene regulation...
  77. Chromatin Pathology in Cerebral Cortex of Schizophrenics
    Schahram Akbarian; Fiscal Year: 2006
    ..Chromatin immunoprecipitation assays (ChIP), in combination with DNA microarrays for promoter and other 5'regulatory gene sequences (chip-on-ChlP), are a ..
  78. Enhancing ENCODE Through a Transcription Factor Tagging Approach to ChIP-seq
    Kevin P White; Fiscal Year: 2011
    ..chromosome (BAC) recombineering to epitope tag 40 transcription factors per year for chromatin immunoprecipitation (ChIP) followed by sequencing to map binding sites genome wide...
  79. Development of a universal tagging method for genome wide ChIP analyses
    ZHENGHE contact WANG; Fiscal Year: 2010
    The techniques of chromatin immunoprecipitation coupled with DNA microarray (ChIP-chip) and sequencing (ChIP-seq) have gained widespread popularity among the greater scientific community...
  80. Microfluidic-microtiter interface for rapid large-scale screenings of C. elegans
    Adela Ben Yakar; Fiscal Year: 2010
    ..has finally enabled us to severe axons of this small worm with high precision, the microfluidic immobilization chip has enabled rapid trapping of worms for surgery without using any anesthetics, reducing the time required to ..
  81. Genomic analysis of pediatric SIRS and septic shock
    Hector Wong; Fiscal Year: 2009
    ..develop a point-of-care platform for measuring serum zinc concentrations in children with septic shock, the "zinc chip." The rationale for development of the zinc chip stems directly from data generated through the translational ..
  82. Nanoliter Lab-on-a-chip for Protein Crystallization
    Vamsee Pamula; Fiscal Year: 2007
    ..unreadable] [unreadable] In phase I, we have successfully demonstrated a protein crystallization lab-on-a-chip using 25 nanoliter protein droplets of 3 proteins viz., lysozyme, glucose isomerase, and proteinase K...
  83. Telemetric Implantable Intraocular Pressure Sensor Syste
    LAWRENCE WAN; Fiscal Year: 2003
    ..An original MEMS lOP sensor chip will be designed and fabricated which will employ a capacitive resonance and resistance effect, and the interface ..
  84. High Throughput Genome Sequencer for Organ Building
    Richard Maas; Fiscal Year: 2009
    ..The Genome Analyzer II requested is ideally suited to support the extensive SAGE and ChIP-Seq analyses now commencing as part of this Consortium effort...
  85. Lab-on-a-chip for multiplexed newborn screening of lysosomal storage disorders
    Vamsee Pamula; Fiscal Year: 2007
    ..microfluidic manipulation of nanoliter-sized droplets of enzymatic reagents and sample for dispensing from on-chip reservoirs, high speed transport, mixing, splitting and dilution, and absorbance, fluorescence, and luminescence ..
  86. Development of a disposable shippable chip for high throughput ion channel screen
    JASON POULOS; Fiscal Year: 2009
    ..Our ultimate goal is to create an inexpensive, disposable chip containing arrays of lipid membranes to enable low cost high throughput screening of ion channels...
  87. A Low Cost Microarray for Population-Scale AIDS Risk Analysis: The AIDS Chip
    Michael E Hogan; Fiscal Year: 2010
    ..technology that we have invented to develop a specialized, very low cost microarray test, referred to as the "AIDS-Chip," which will perform very-high-resolution HLA-B testing as a single, simple, inexpensive, compact microarray test...
  88. Nuclear Receptor Networks in Human Disease
    Frances M Sladek; Fiscal Year: 2010
    ..Whereas recent advances in whole genome expression profiling and genome-wide location analysis (ChIP-chip, ChIP-seq) have allowed us to begin to define the complete transcriptome for some of these factors, much more ..
  89. GnRH-Regulated Transcriptome in the Gonadotrope
    Djurdjica Coss; Fiscal Year: 2010
    ..the complement of direct target genes, in addition to gonadotropin subunits, of these immediate early genes by ChIP-chip, which will allow us to delineate the transcriptional regulatory network...
  90. AFM Sensors to Detect Biological Warfare Agents
    Curtis Mosher; Fiscal Year: 2002
    ..BioForce's NanoArray chip technology is based on affinity capture of pathogens using solid phase antibody arrays and Atomic Force Microscopy (..
  91. ANALYSIS OF CHROMATIN REMODELING IN MAMMARY GLAND DEVELOPMENT USING TILING ARRAYS
    Monique Rijnkels; Fiscal Year: 2009
    ..A global approach to this problem is now possible with the recent development of ChIP-chip, a new technology combining Chromatin Immuno-precipitation (ChIP) and high-throughput oligonucleotide-tiling ..
  92. Molecular and Cellular Basis of Oncogene Addiction
    Dean W Felsher; Fiscal Year: 2010
    ..are frequently accompanied by permanent changes in the ability of MYC binding to promoter loci, as shown by ChIP. We performed a preliminary ChIP-on-chip analysis for MYC and interrogated changes in binding of other ..
  93. ON-CHIP DNA PURIFICATION/PCR FOR CANCER DIAGNOSTICS
    James Landers; Fiscal Year: 2000
    ..methodological "bottleneck" can be solved, in part, by capillary electrophoresis (CE) and more comprehensively by "chip electrophoresis"...
  94. INFLUENCE OF AGING ON MITOCHONDRIAL GENE EXPRESSION FOLLOWING TRAUMA-HEMORRHAGE
    Raghavan Raju; Fiscal Year: 2009
    ..gene expression in the heart, which can be determined in an experimental model using a mitochondrial gene chip. The objective of this proposal is to develop and validate a rodent mitochondrial gene chip and use it to determine ..