CYBB

Summary

Gene Symbol: CYBB
Description: cytochrome b-245 beta chain
Alias: AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX, cytochrome b-245 heavy chain, CGD91-phox, NADPH oxidase 2, cytochrome b(558) subunit beta, cytochrome b-245 beta polypeptide, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, neutrophil cytochrome b 91 kDa polypeptide, p22 phagocyte B-cytochrome, superoxide-generating NADPH oxidase heavy chain subunit
Species: human

Top Publications

  1. pmc Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex
    Chunyan Ma
    Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 287:18359-65. 2012
  2. doi Inhibition of nuclear factor erythroid 2-related factor 2 exacerbates HIV-1 gp120-induced oxidative and inflammatory response: role in HIV associated neurocognitive disorder
    Pichili Vijaya Bhaskar Reddy
    Department of Immunology, Institute of Neuroimmune Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA
    Neurochem Res 37:1697-706. 2012
  3. pmc Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis
    Johan Aurelius
    Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden
    Blood 119:5832-7. 2012
  4. pmc Broad suppression of NADPH oxidase activity exacerbates ischemia/reperfusion injury through inadvertent downregulation of hypoxia-inducible factor-1α and upregulation of peroxisome proliferator-activated receptor-α
    Shouji Matsushima
    Department of Cell Biology and Molecular Medicine, Cardiovascular Research Institute, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Circ Res 112:1135-49. 2013
  5. pmc Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta)-dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane
    Fredrick D Oakley
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 284:33255-64. 2009
  6. doi Involvement of RAGE, NADPH oxidase, and Ras/Raf-1 pathway in glycated LDL-induced expression of heat shock factor-1 and plasminogen activator inhibitor-1 in vascular endothelial cells
    Ganesh V Sangle
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P4
    Endocrinology 151:4455-66. 2010
  7. ncbi Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location
    B Royer-Pokora
    Nature 322:32-8. 1986
  8. ncbi Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production
    J D Pollock
    Department of Pediatrics, James Whitcomb Riley Hospital for Children, Indianapolis, Indiana, USA
    Nat Genet 9:202-9. 1995
  9. ncbi Expression and localization of NOX2 and NOX4 in primary human endothelial cells
    J D Van Buul
    Sanquin Research at CLB and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Antioxid Redox Signal 7:308-17. 2005
  10. pmc Nox2 and Rac1 regulate H2O2-dependent recruitment of TRAF6 to endosomal interleukin-1 receptor complexes
    Qiang Li
    Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA 52242, USA
    Mol Cell Biol 26:140-54. 2006

Research Grants

  1. Biosynthesis, structural organization, and function of NOX proteins
    WILLIAM NAUSEEF; Fiscal Year: 2007
  2. Redox regulation of phagocyte NOX2 in inflammation and aging
    Robert A Clark; Fiscal Year: 2013
  3. Taylor W Starnes; Fiscal Year: 2014
  4. Macrophage PPARg signaling, efferocytosis, and exaggerated inflammation in CGD
    Donna L Bratton; Fiscal Year: 2011
  5. Function of NADPH Oxidase(s) in T Lymphocytes
    Mark S Williams; Fiscal Year: 2010
  6. Novel Approach to Oral Gene Therapy for Chronic Granulomatous Disease
    Peter E Newburger; Fiscal Year: 2010
  7. Role of NADPH Oxidase in Regulating Inflammation
    Brahm H Segal; Fiscal Year: 2013
  8. The role of neutrophil extracellular traps and NADPH oxidase in SLE pathogenesis
    ALLISON MARIE CAMPBELL; Fiscal Year: 2012
  9. MOLECULAR MECHANISMS OF LEUKOCYTE ACTIVATION
    Richard Ye; Fiscal Year: 2009
  10. Richard D Ye; Fiscal Year: 2014

Detail Information

Publications305 found, 100 shown here

  1. pmc Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex
    Chunyan Ma
    Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 287:18359-65. 2012
    ..Slamf1 induces production of phosphatidylinositol 3-phosphate, which positively regulates the activity of the NOX2 enzyme and phagolysosomal maturation...
  2. doi Inhibition of nuclear factor erythroid 2-related factor 2 exacerbates HIV-1 gp120-induced oxidative and inflammatory response: role in HIV associated neurocognitive disorder
    Pichili Vijaya Bhaskar Reddy
    Department of Immunology, Institute of Neuroimmune Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA
    Neurochem Res 37:1697-706. 2012
    ..Our results indicate that when astrocytes are exposed to gp120 there is an increase in the expression of NOX2, a subunit of NADPH oxidase, and also an upregulated expression of nuclear factor kappa B, tumor necrosis factor-α ..
  3. pmc Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis
    Johan Aurelius
    Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden
    Blood 119:5832-7. 2012
    ..Our data are suggestive of a novel mechanism by which monocytic AML cells evade cell-mediated immunity...
  4. pmc Broad suppression of NADPH oxidase activity exacerbates ischemia/reperfusion injury through inadvertent downregulation of hypoxia-inducible factor-1α and upregulation of peroxisome proliferator-activated receptor-α
    Shouji Matsushima
    Department of Cell Biology and Molecular Medicine, Cardiovascular Research Institute, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Circ Res 112:1135-49. 2013
    ..The isoform-specific contribution of Nox2 and Nox4 to ischemia/reperfusion (I/R) injury is poorly understood.
  5. pmc Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta)-dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane
    Fredrick D Oakley
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 284:33255-64. 2009
    ..receptor (IL-1R1) following ligand binding and the formation of redox-active signaling endosomes that contain Nox2 (also called redoxosomes)...
  6. doi Involvement of RAGE, NADPH oxidase, and Ras/Raf-1 pathway in glycated LDL-induced expression of heat shock factor-1 and plasminogen activator inhibitor-1 in vascular endothelial cells
    Ganesh V Sangle
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P4
    Endocrinology 151:4455-66. 2010
    ..Small interference RNA for p22(phox) prevented glyLDL-induced expression of NOX2, HSF1, and PAI-1 in EC...
  7. ncbi Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location
    B Royer-Pokora
    Nature 322:32-8. 1986
    ..The nucleotide sequence of complementary DNA clones predicts a polypeptide of at least 468 amino acids with no homology to proteins described previously...
  8. ncbi Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production
    J D Pollock
    Department of Pediatrics, James Whitcomb Riley Hospital for Children, Indianapolis, Indiana, USA
    Nat Genet 9:202-9. 1995
    Chronic granulomatous disease (CGD) is a recessive disorder characterized by a defective phagocyte respiratory burst oxidase, life-threatening pyogenic infections and inflammatory granulomas...
  9. ncbi Expression and localization of NOX2 and NOX4 in primary human endothelial cells
    J D Van Buul
    Sanquin Research at CLB and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Antioxid Redox Signal 7:308-17. 2005
    ..Here we show that HUVECs express mRNAs for NOX2 as well as NOX4 mRNA, but not for NOX1 or NOX3...
  10. pmc Nox2 and Rac1 regulate H2O2-dependent recruitment of TRAF6 to endosomal interleukin-1 receptor complexes
    Qiang Li
    Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA 52242, USA
    Mol Cell Biol 26:140-54. 2006
    ..We have found that Nox2-derived ROS influence the formation of an active interleukin-1 (IL-1) receptor complex in the endosomal compartment ..
  11. ncbi NOX2 and NOX4 mediate proliferative response in endothelial cells
    Andreas Petry
    Experimental Pediatric Cardiology, Department of Pediatric Cardiology and Congenital Heart Diseases, German Heart Center Munich at the Technical University Munich, Munich, Germany
    Antioxid Redox Signal 8:1473-84. 2006
    ..In neutrophils, ROS are generated by a NADPH oxidase containing p22phox and NOX2. NADPH oxidases are also major sources of vascular ROS...
  12. ncbi Endothelial Nox2 overexpression potentiates vascular oxidative stress and hemodynamic response to angiotensin II: studies in endothelial-targeted Nox2 transgenic mice
    Jennifer K Bendall
    Department of Cardiovascular Medicine, University of Oxford, John Radcliffe Hospital, OX39DU, UK
    Circ Res 100:1016-25. 2007
    ..questions using transgenic mice with endothelial-targeted overexpression of the catalytic subunit of NADPH oxidase, Nox2. Aortas of Nox2 transgenic (Nox2-Tg) mice had increased total Nox2 mRNA and protein levels compared with wild-type ..
  13. pmc Nox2-derived radicals contribute to neurovascular and behavioral dysfunction in mice overexpressing the amyloid precursor protein
    Laibaik Park
    Division of Neurobiology, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:1347-52. 2008
    ..We found that 12- to 15-month-old Tg2576 mice lacking the catalytic subunit Nox2 of NADPH oxidase do not develop oxidative stress, cerebrovascular dysfunction, or behavioral deficits...
  14. doi Nox4 and nox2 NADPH oxidases mediate distinct cellular redox signaling responses to agonist stimulation
    Narayana Anilkumar
    Department of Cardiology, The James Black Centre, King s College London, 125 Coldharbour Lane, London SE59NU, UK
    Arterioscler Thromb Vasc Biol 28:1347-54. 2008
    The NADPH oxidase isoforms Nox2 and Nox4 are coexpressed in many cell types and are implicated in agonist-stimulated redox-sensitive signal transduction...
  15. doi NADPH oxidase isoform selective regulation of endothelial cell proliferation and survival
    Hitesh Peshavariya
    Bernard O Brien Institute of Microsurgery, The University of Melbourne, Fitzroy, Victoria, Australia
    Naunyn Schmiedebergs Arch Pharmacol 380:193-204. 2009
    ..In the present study, we investigated the roles of the ROS-generating Nox4- and Nox2-containing reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases in proliferation of human ..
  16. doi Hereditary deficiency of gp91(phox) is associated with enhanced arterial dilatation: results of a multicenter study
    Francesco Violi
    Department of Experimental Medicine, Divisione I Clinica Medica, University of Rome La Sapienza, Rome, Italy
    Circulation 120:1616-22. 2009
    ..NADPH oxidase is believed to modulate arterial tone, but its role in humans is still unclear. The objective of this study was to evaluate whether NADPH oxidase is involved in flow-mediated arterial dilation (FMD)...
  17. doi Atorvastatin inhibits gp91phox circulating levels in patients with hypercholesterolemia
    Pasquale Pignatelli
    Department of Experimental Medicine, Divisione I Clinica Medica, Sapienza University, Rome, Italy
    Arterioscler Thromb Vasc Biol 30:360-7. 2010
    ..01), that paralleled that of isoprostanes (-37%, P<0.01) and cholesterol (-25%, P<0.01). The diet-allocated group showed only a weak reduction of cholesterol...
  18. pmc Hematologically important mutations: X-linked chronic granulomatous disease (third update)
    Dirk Roos
    Sanquin Research, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Plesmanlaan 125, 1066 CX, Amsterdam, The Netherlands
    Blood Cells Mol Dis 45:246-65. 2010
    ..leukocyte NADPH oxidase is composed of five subunits, of which the enzymatic component is gp91-phox, also called Nox2. This protein is encoded by the CYBB gene on the X chromosome...
  19. doi Inherited human gp91phox deficiency is associated with impaired isoprostane formation and platelet dysfunction
    Pasquale Pignatelli
    Department of Internal Medicine and Medical Specialties, I Clinica Medica, University of Rome La Sapienza, Viale del Policlinico 155, Rome, Italy
    Arterioscler Thromb Vasc Biol 31:423-34. 2011
    ..We hypothesized that NADPH is implicated in isoprostane formation and platelet activation...
  20. doi Nox2-based NADPH oxidase mediates HIV-1 Tat-induced up-regulation of VCAM-1/ICAM-1 and subsequent monocyte adhesion in human astrocytes
    Ha Yong Song
    Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200 702, Republic of Korea
    Free Radic Biol Med 50:576-84. 2011
    ..b>Nox2 knockdown using small interfering RNA (siRNA) inhibited HIV-1 Tat-induced up-regulation of adhesion molecules and ..
  21. doi Obesity and hypercholesterolemia are associated with NOX2 generated oxidative stress and arterial dysfunction
    Lorenzo Loffredo
    I Clinica Medica, Sapienza University, Rome, Italy
    J Pediatr 161:1004-9. 2012
    To analyze the interplay among oxidative stress, NOX2, the catalytic core of nicotinamide-adenine dinucleotide phosphate oxidase, and endothelial dysfunction in children with obesity and/or hypercholesterolemia.
  22. doi Does NADPH oxidase deficiency cause artery dilatation in humans?
    Lorenzo Loffredo
    Divisione I Clinica Medica, Department of Internal Medicine and Medical Specialities, University of Rome La Sapienza, Rome, Italy
    Antioxid Redox Signal 18:1491-6. 2013
    ..The objective of this study was to compare the role of p47 and gp91phox (NOX2) on artery dilatation...
  23. doi Reduced atherosclerotic burden in subjects with genetically determined low oxidative stress
    Francesco Violi
    Clinica Medica I, Sapienza University of Rome, Rome, Italy
    Arterioscler Thromb Vasc Biol 33:406-12. 2013
    ..We hypothesized that a reduced activity of NADPH oxidase might be linked to a reduced atherosclerotic burden...
  24. pmc Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II
    Pham My Chan Dang
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:4262-5. 2002
    ....
  25. ncbi Leu505 of Nox2 is crucial for optimal p67phox-dependent activation of the flavocytochrome b558 during phagocytic NADPH oxidase assembly
    Xing Jun Li
    Groupe de Recherche et d Etude du Processus Inflammatoire, Universite Joseph Fourier, Laboratoire d Enzymologie, Centre Hospitalier Universitaire, Grenoble CHU 38043, Cedex 9, France
    J Leukoc Biol 81:238-49. 2007
    The role of Leu505 of Nox2 on the NADPH oxidase activation process was investigated. An X-CGD PLB-985 cell line expressing the Leu505Arg Nox2 mutant was obtained, exactly mimicking the phenotype of a previously published X91+-CGD case...
  26. doi iPLA2, a novel determinant in Ca2+- and phosphorylation-dependent S100A8/A9 regulated NOX2 activity
    Véronique Schenten
    Life Sciences Research Unit, University of Luxembourg, 162A Avenue de la Faïencerie, L 1511 Luxembourg
    Biochim Biophys Acta 1803:840-7. 2010
    The neutrophil NADPH oxidase (NOX2) is a key enzyme responsible for host defense against invading pathogens, via the production of reactive oxygen species...
  27. doi Akt/Nox2/NF-κB signaling pathway is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
    Hong Sheng Zhang
    Beijing University of Technology, District of Chaoyang, China
    Arch Biochem Biophys 505:266-72. 2011
    ..caused increases in ROS formation that were prevented by both pharmacologic NADPH oxidase inhibitors and by siRNA Nox2, but not by other inhibitors of pro-oxidant enzymes or siRNA Nox4...
  28. ncbi HIV-1 Tat activates dual Nox pathways leading to independent activation of ERK and JNK MAP kinases
    Ru Feng Wu
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 282:37412-9. 2007
    ..oxidase inhibitor diphenylene iodonium blocked ERK phosphorylation, but specific interference with the canonical Nox2 oxidase had no effect on ERK...
  29. pmc X-linked chronic granulomatous disease secondary to skewed X chromosome inactivation in a female with a novel CYBB mutation and late presentation
    Eric M Lewis
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Clin Immunol 129:372-80. 2008
    ..We found a null mutation in the CYBB gene (c.252insAG) predicting an aberrant gp91(phox) protein (p.Cys85fsX23) in the heterozygous state...
  30. doi Determination of the deletion breakpoints in two patients with contiguous gene syndrome encompassing CYBB gene
    Masafumi Yamada
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, North 15 West 7, Kita ku Sapporo 060 8638, Japan
    Eur J Med Genet 53:383-8. 2010
    X-linked chronic granulomatous disease is a primary immunodeficiency caused by mutations in CYBB. Although large deletions involving CYBB are known to cause contiguous gene syndrome (CGS), only a few patients have been studied precisely ..
  31. doi Coupling of 6-phosphogluconate dehydrogenase with NADPH oxidase in neutrophils: Nox2 activity regulation by NADPH availability
    Athan Baillet
    Groupe de Recherche et d Etude du Processus Inflammatoire GREPI, Centre National de la Recherche Scientifique CNRS, Université Joseph Fourier UJF, Grenoble, France
    FASEB J 25:2333-43. 2011
    ..Interestingly, the Michaelis constant (K(m)) of Nox2 for NADPH increases in 6PGDH-depleted cells...
  32. ncbi Nonhomologous recombination between the cytochrome b558 heavy chain gene (CYBB) and LINE-1 causes an X-linked chronic granulomatous disease
    A Kumatori
    Institute of Tropical Medicine, Nagasaki University, Nagasaki, 852 8523, Japan
    Genomics 53:123-8. 1998
    ..junction of a chronic granulomatous disease patient with a 25-kb deletion extending to the 5' two-thirds of CYBB. The 3' breakpoint of the deletion exists in exon 7 of CYBB...
  33. ncbi Analysis of human phagocyte flavocytochrome b(558) by mass spectrometry
    Ross M Taylor
    Departments of Microbiology and Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    J Biol Chem 281:37045-56. 2006
    ....
  34. doi Involvement of NADPH oxidase in oxidized LDL-induced upregulation of heat shock factor-1 and plasminogen activator inhibitor-1 in vascular endothelial cells
    Ruozhi Zhao
    Diabetes Research Group, University of Manitoba, 835 715 McDermot Ave, Winnipeg, Manitoba R3E 3P4, Canada
    Am J Physiol Endocrinol Metab 297:E104-11. 2009
    ..RNA (siRNA) for p22(phox), an essential subunit of NOX, prevented oxidized LDL-induced expression of NOX2, HSF1, and PAI-1 in EC. HSF1 siRNA inhibited oxidized LDL-induced expression of PAI-1 and HSF1, but not NOX2, in EC...
  35. ncbi A splice-supporting intronic mutation in the last bp position of a cryptic exon within intron 6 of the CYBB gene induces its incorporation into the mRNA causing chronic granulomatous disease (CGD)
    Andreas Rump
    Institute of Human and Clinical Genetics, University Clinic Carl Gustav Carus, Dresden, Germany
    Gene 371:174-81. 2006
    ..due to a splice-supporting mutation in the last position of a cryptic exon towards the middle of intron 6 of the CYBB (gp91-phox) gene...
  36. ncbi [An autopsy case of chronic granulomatous disease diagnosed by biopsy]
    H Gonda
    Department of Respiratory Medicine and Allergy, Toyohashi Municipal Hospital
    Kansenshogaku Zasshi 71:668-71. 1997
    ..Autopsy demonstrated pigmented lipid histiocytes characteristic of CGD. These characteristic pigmented cells were distributed in the spleen, liver, lymph nodes and in the small intestine...
  37. pmc Regulation of NADPH oxidase activity in phagocytes: relationship between FAD/NADPH binding and oxidase complex assembly
    Franck Debeurme
    Chronic Granulomatous Disease Diagnosis and Research Center, University Hospital Grenoble, TheREx TIMC Imag UMR CNRS 5525, Universite Joseph Fourier, 38043 Grenoble Cedex 9
    J Biol Chem 285:33197-208. 2010
    ..disease (X(+)-CGD) variants are natural mutants characterized by defective NADPH oxidase activity but with normal Nox2 expression...
  38. ncbi Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers
    M de Boer
    Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam
    Blood 83:531-6. 1994
    Chronic granulomatous disease (CGD) is characterized by the failure of phagocytic leukocytes to kill certain bacteria and fungi...
  39. pmc Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    BMC Evol Biol 7:178. 2007
    ..The prototypical subunit-regulated Nox, Nox2, is the membrane-associated catalytic subunit of the phagocyte NADPH-oxidase...
  40. doi Pharmacological and genetic inhibition of NADPH oxidase does not reduce brain damage in different models of perinatal brain injury in newborn mice
    Christina Doverhag
    Department of Physiology and Neuroscience, Perinatal Center, Sahlgrenska Academy, Goteborg University, Sweden
    Neurobiol Dis 31:133-44. 2008
    ..The ROS-generating enzyme NADPH oxidase (Nox2) is present in inflammatory cells and contributes to brain injury in adult animal models.
  41. pmc Role of NADPH oxidase in retinal vascular inflammation
    Mohamed Al-Shabrawey
    Oral Biology and Anatomy, School of Dentistry, Medical College of Georgia, Augusta, Georgia 30912, USA
    Invest Ophthalmol Vis Sci 49:3239-44. 2008
    ..ROS, VEGF expression, and vascular permeability are accompanied by increases in the NADPH oxidase catalytic subunit NOX2 within the retinal vessels...
  42. doi NADPH oxidase activity is higher in cerebral versus systemic arteries of four animal species: role of Nox2
    Alyson A Miller
    Dept of Pharmacology, Monash Univ, Clayton, Melbourne, Victoria 3800, Australia
    Am J Physiol Heart Circ Physiol 296:H220-5. 2009
    ..e., mouse, rabbit, and pig. Second, using Nox2-deficient mice, we evaluated the involvement of Nox2-containing NADPH oxidases in any such regional differences...
  43. doi Priming of the neutrophil NADPH oxidase activation: role of p47phox phosphorylation and NOX2 mobilization to the plasma membrane
    Jamel El-Benna
    INSERM, U773, CRB3, Paris 75018, France
    Semin Immunopathol 30:279-89. 2008
    ..This multicomponent enzyme system is composed of two trans-membrane proteins (p22phox and gp91phox/NOX2, which form the cytochrome b558), three cytosolic proteins (p47phox, p67phox, p40phox) and a GTPase (Rac1 or ..
  44. ncbi Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking"
    Iris Dahan
    Julius Friedrich Cohnheim Minerva Center for Phagocyte Research and the Ela Kodesz Institute of Host Defense against Infectious Diseases, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 277:8421-32. 2002
    ..A revised membrane topology model of p22(phox) is proposed, the core of which is the presence of a functionally important cytosolic loop (residues 51-91)...
  45. pmc Reactive oxygen species regulate neutrophil recruitment and survival in pneumococcal pneumonia
    Helen M Marriott
    Section of Infection and Inflammation, School of Medicine and Biomedical Sciences, University of Sheffield, LU107, Royal Hallamshire Hospital, Glossop Road, Sheffield, S10 2JF, UK
    Am J Respir Crit Care Med 177:887-95. 2008
    ..The relative importance of each role remains poorly defined in community-acquired pneumonia...
  46. ncbi gp91phox-containing NADPH oxidase mediates endothelial dysfunction in renovascular hypertension
    O Jung
    Institut für Kardiovaskuläre Physiologie, Klinikum der J W Goethe Universitat, Frankfurt am Main, Germany
    Circulation 109:1795-801. 2004
    ..We hypothesized that the endothelial gp91phox-containing NADPH oxidase is predominant in generating the O2- to scavenge endothelial NO and thus is responsible ..
  47. ncbi Characteristics of NADPH oxidase genes (Nox2, p22, p47, and p67) and Nox4 gene expressed in blood cells of juvenile Ciona intestinalis
    Yuuki Inoue
    Laboratory of Fish Pathology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252 8510, Japan
    Immunogenetics 57:520-34. 2005
    To illuminate the origins of NADPH oxidase (Nox), we identified cDNA clones encoding Nox2, Nox4, p22 phagocyte oxidase (phox), p47phox, and p67phox in a chordate phylogenetically distant to the vertebrates, the sea squirt Ciona ..
  48. pmc NADPH oxidase inhibition improves neurological outcomes in surgically-induced brain injury
    Wendy Lo
    Department of Physiology, Loma Linda University and Loma Linda University School of Medicine, Loma Linda, CA 92350, USA
    Neurosci Lett 414:228-32. 2007
    ..oxidase, an important reactive oxygen species producing enzyme, is involved in SBI using transgenic mice lacking gp91phox subunit of NADPH oxidase (gp91phox KO) and apocynin, a specific inhibitor of NADPH oxidase...
  49. ncbi Gp91phox contributes to NADPH oxidase activity in aortic fibroblasts but not smooth muscle cells
    Ali H Chamseddine
    Department of Internal Medicine and Free Radical and Radiation Biology Program, University of Iowa, Iowa City, IA 52242, USA
    Am J Physiol Heart Circ Physiol 285:H2284-9. 2003
    ..We examined the functional expression of gp91phox on NADPH oxidase activity in vascular smooth muscle cells (SMC) and fibroblasts (FB)...
  50. ncbi New insights into the membrane topology of the phagocyte NADPH oxidase: characterization of an anti-gp91-phox conformational monoclonal antibody
    Yannick Campion
    GREPI EA 2938, Lab Enzymologie DBPC, CHU Grenoble, BP 217, 38043 Grenoble Cedex 9, France
    Biochimie 89:1145-58. 2007
    ..Moreover, the antibody 13B6 may be conformationally sensitive and used as a probe for identifying the active NADPH oxidase complex in vivo...
  51. ncbi Implication of cytosolic phospholipase A2 (cPLA2) in the regulation of human synoviocyte NADPH oxidase (Nox2) activity
    Camille Chenevier-Gobeaux
    Laboratoire de Biochimie A, Hopital Cochin, Assistance Publique Hopitaux de Paris AP HP, 27 rue du Faubourg Saint Jacques, 75679 Paris Cedex 14, France
    Life Sci 81:1050-8. 2007
    NADPH oxidase Nox2 is involved in the production of superoxide by rheumatoid synovial cells, constitutively and after pro-inflammatory cytokine treatment...
  52. ncbi Ischemic stroke injury is reduced in mice lacking a functional NADPH oxidase
    C E Walder
    Department of Cardiovascular Research, Genentech Inc, South San Francisco, Calif, USA
    Stroke 28:2252-8. 1997
    ..Using mutant mice (X-CGD) with a dysfunctional phagocytic NADPH oxidase, we investigated the role of this superoxide-generating enzyme as a ..
  53. pmc H2S-donating sildenafil (ACS6) inhibits superoxide formation and gp91phox expression in arterial endothelial cells: role of protein kinases A and G
    S Muzaffar
    Department of Cardiac Surgery, Bristol Royal Infirmary, Bristol Heart Institute, University of Bristol, Bristol, UK
    Br J Pharmacol 155:984-94. 2008
    ....
  54. ncbi Chronic granulomatous disease in pediatric patients: 25 years of experience
    P Soler-Palacín
    Immunology Unit, Vall d Hebron University Hospital, Barcelona, Spain
    Allergol Immunopathol (Madr) 35:83-9. 2007
    Chronic granulomatous disease (CGD) is an uncommon primary immune deficiency (affecting 1/200,000 newborn infants) caused by a defect in phagocyte production of oxygen metabolites, and resulting in bacterial infections produced by ..
  55. pmc Genes differentially expressed in conidia and hyphae of Aspergillus fumigatus upon exposure to human neutrophils
    Janyce A Sugui
    Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 3:e2655. 2008
    ..fumigatus in response to neutrophils from healthy donors as well as from those with chronic granulomatous disease (CGD) which are defective in the production of reactive oxygen species.
  56. pmc O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase
    S L Archer
    Department of Medicine, Division of Cardiology, University of Alberta, Edmonton, Canada T69 2B7
    Proc Natl Acad Sci U S A 96:7944-9. 1999
    ..In wild-type lungs, gp91 phox and p22 phox subunits are present (relative expression: macrophages > airways and veins > PASMCs)...
  57. ncbi Role of NADPH oxidase in the mechanism of lung neutrophil sequestration and microvessel injury induced by Gram-negative sepsis: studies in p47phox-/- and gp91phox-/- mice
    Xiao Pei Gao
    Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL 60612, USA
    J Immunol 168:3974-82. 2002
    ....
  58. ncbi Direct interaction of the novel Nox proteins with p22phox is required for the formation of a functionally active NADPH oxidase
    Rashmi K Ambasta
    Institut für Kardiovaskuläre Physiologie, Klinikum der J W Goethe Universitat, D 60596 Frankfurt am Main, Germany
    J Biol Chem 279:45935-41. 2004
    Nox1 and Nox4, homologues of the leukocyte NADPH oxidase subunit Nox2 (gp91phox) mediate superoxide anion formation in various cell types. However, their interactions with other components of the NADPH oxidase are poorly defined...
  59. ncbi Aldosterone mediates angiotensin II-induced interstitial cardiac fibrosis via a Nox2-containing NADPH oxidase
    Sofian Johar
    King s College London, Cardiovascular Division, London, UK
    FASEB J 20:1546-8. 2006
    ..We investigated the potential role of a Nox2-containing NADPH oxidase in aldosterone-induced fibrosis and the involvement of this mechanism in AngII-induced ..
  60. doi A region N-terminal to the tandem SH3 domain of p47phox plays a crucial role in the activation of the phagocyte NADPH oxidase
    Masahiko Taura
    Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem J 419:329-38. 2009
    ..crucial for host defence; its catalytic core is the membrane-integrated protein gp91phox [also known as Nox2 (NADPH oxidase 2)], which forms a stable heterodimer with p22phox...
  61. doi The anti-platelet effects of apocynin in mice are not mediated by inhibition of NADPH oxidase activity
    Janahan Dharmarajah
    Department of Pharmacology, Monash University, Clayton, Victoria, Australia
    Naunyn Schmiedebergs Arch Pharmacol 382:377-84. 2010
    ..Washed platelets were isolated from male C57BL6 (wild-type), Nox2-deficient (Nox2(-/y )), and p47phox-deficient (p47phox(-/-)) mice for assessment of aggregation and NADPH oxidase ..
  62. ncbi NF-kappaB regulates phagocytic NADPH oxidase by inducing the expression of gp91phox
    Josef Anrather
    Division of Neurobiology, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Biol Chem 281:5657-67. 2006
    ..The findings raise the possibility of a positive feedback loop in which NF-kappaB activation by oxidative stress leads to further radical production via NADPH oxidase...
  63. doi Overlap, common features, and essential differences in pediatric granulomatous inflammatory bowel disease
    Gerard M Damen
    Department of Paediatrics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Pediatr Gastroenterol Nutr 51:690-7. 2010
    ..Chronic granulomatous disease (CGD) may present with granulomatous colitis, perianal abscesses, hepatic abscesses or granulomas, failure to thrive, ..
  64. ncbi Skewing of X-chromosome inactivation in three generations of carriers with X-linked chronic granulomatous disease within one family
    M Y Köker
    Division of Pediatric Immunology, University of Hacettepe, Ankara, Turkey
    Eur J Clin Invest 36:257-64. 2006
    ..Defects in gp91-phox, encoded by CYBB, lead to X-linked CGD, responsible for approximately 70% of all CGD cases...
  65. ncbi [Effect of fluconazole on Aspergillus infection associated with chronic granulomatous disease]
    J Kawamori
    Division of Allergy, Clinical Immunology and Infectious Disease, Shizuoka Children's Hospital
    Kansenshogaku Zasshi 65:1200-4. 1991
    Aspergillus infection is the most frequent fungal infection associated with chronic granulomatous disease (CGD), and often results in a life-threatening situation...
  66. ncbi Enhanced cutaneous inflammatory reactions to Aspergillus fumigatus in a murine model of chronic granulomatous disease
    Jeffrey E Petersen
    Department of Dermatology, Indiana University School of Medicine, Indianapolis, Indiana, USA
    J Invest Dermatol 118:424-9. 2002
    ..chronic granulomatous disease (X-CGD) created by homologous recombinant disruption of the gene encoding the gp91phox component of the NADPH oxidase, in this study we examined cutaneous reactivity to sterile Aspergillus fumigatus ..
  67. doi Thapsigargin and flavin adenine dinucleotide ex vivo treatment rescues trafficking-defective gp91phox in chronic granulomatous disease leukocytes
    Ya Fang Huang
    Institute of Basic Medicine, National Cheng Kung University Medical College, Tainan 704, Taiwan
    Free Radic Biol Med 47:932-40. 2009
    ..The most common form of CGD is caused by mutations in the membrane-bound oxidase component gp91phox, which is encoded by the CYBB gene on the X chromosome...
  68. pmc Virulence of catalase-deficient aspergillus nidulans in p47(phox)-/- mice. Implications for fungal pathogenicity and host defense in chronic granulomatous disease
    Y C Chang
    Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 101:1843-50. 1998
    Chronic granulomatous disease (CGD) is a rare genetic disorder in which phagocytes fail to produce superoxide because of defects in one of several components of the NADPH oxidase complex...
  69. ncbi Complementation of NADPH oxidase in p67-phox-deficient CGD patients p67-phox/p40-phox interaction
    S Vergnaud
    GREPI, Laboratoire d Enzymologie, CHU, Grenoble, France INSERM U 479, CHU, Bichat, Paris, France
    Eur J Biochem 267:1059-67. 2000
    Chronic granulomatous disease (CGD) is due to a functional defect of the O2- generating NADPH oxidase of phagocytes...
  70. ncbi Cytokine imbalance in hyper-IgE syndrome: reduced expression of transforming growth factor beta and interferon gamma genes in circulating activated T cells
    Shouichi Ohga
    Department of Paediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Br J Haematol 121:324-31. 2003
    ..of naturally activated T cells was compared between HIES, atopic dermatitis and chronic granulomatous disease (CGD)...
  71. ncbi Molecular basis of the autosomal recessive forms of chronic granulomatous disease
    J T Curnutte
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037 1093
    Immunodefic Rev 3:149-72. 1992
    Chronic granulomatous disease (CGD) is an inherited group of disorders in which phagocytic leukocytes (neutrophils, eosinophils, monocytes, and macrophages) fail to undergo a respiratory burst when stimulated...
  72. ncbi Retroviral-mediated gene transfer of gp91phox into bone marrow cells rescues defect in host defense against Aspergillus fumigatus in murine X-linked chronic granulomatous disease
    H Björgvinsdóttir
    Herman B Wells Center for Pediatric Research, Department of Pediatrics Hematology Oncology, James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis, USA
    Blood 89:41-8. 1997
    ..an inherited deficiency of the respiratory burst oxidase, results from mutations in the X-linked gene for gp91phox, the larger subunit of the oxidase cytochrome b...
  73. pmc Gamma interferon treatment of patients with chronic granulomatous disease is associated with augmented production of nitric oxide by polymorphonuclear neutrophils
    A Ahlin
    Department of Pediatrics, the Karolinska Institute at Sachs Children s Hospital, S 118 95 Stockholm, Huddinge University Hospital, S 141 86 Huddinge, Sweden
    Clin Diagn Lab Immunol 6:420-4. 1999
    ..IFN-gamma) is associated with reduced frequency and severity of infections in chronic granulomatous disease (CGD), but the mechanism is unknown...
  74. ncbi The expression of full length Gp91-phox protein is associated with reduced amphotropic retroviral production
    I Bellantuono
    Department of Immunology, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London, W12 0NN, United Kingdom
    Haematologica 85:451-7. 2000
    As a single gene defect in mature bone marrow cells, chronic granulomatous disease (X-CGD) represents a disorder which may be amenable to gene therapy by the transfer of the missing subunit into hemopoietic stem cells...
  75. ncbi Human polymorphonuclear leukocytes inhibit Aspergillus fumigatus conidial growth by lactoferrin-mediated iron depletion
    Kol A Zarember
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 178:6367-73. 2007
    ..mold, rarely infects humans, except during prolonged neutropenia or in cases of chronic granulomatous disease (CGD), a primary immunodeficiency caused by mutations in the NADPH oxidase that normally produces fungicidal reactive ..
  76. pmc Actinomyces in chronic granulomatous disease: an emerging and unanticipated pathogen
    Janine Reichenbach
    Division of Immunology, University Children s Hospital, Zurich, Switzerland
    Clin Infect Dis 49:1703-10. 2009
    Chronic granulomatous disease (CGD) is a rare inherited disease of the phagocyte NADPH oxidase system that causes defective production of toxic oxygen metabolites, impaired bacterial and fungal killing, and recurrent life-threatening ..
  77. pmc Cytochrome b558-negative, autosomal recessive chronic granulomatous disease: two new mutations in the cytochrome b558 light chain of the NADPH oxidase (p22-phox)
    M de Boer
    Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam
    Am J Hum Genet 51:1127-35. 1992
    Chronic granulomatous disease (CGD) is characterized by the failure of activated phagocytes to generate superoxide. Defects in at least four different genes lead to CGD...
  78. ncbi Molecular characterization of autosomal recessive chronic granulomatous disease caused by a defect of the nicotinamide adenine dinucleotide phosphate (reduced form) oxidase component p67-phox
    P J Patiño
    Department of Immunology, Genentech, Inc, South San Francisco, CA, USA
    Blood 94:2505-14. 1999
    Chronic granulomatous disease (CGD) is a rare inherited disorder of phagocytes in which defective production of microbicidal oxidants leads to severe recurrent infections...
  79. ncbi Chronic granulomatous disease caused by mutations other than the common GT deletion in NCF1, the gene encoding the p47phox component of the phagocyte NADPH oxidase
    Dirk Roos
    Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    Hum Mutat 27:1218-29. 2006
    Chronic granulomatous disease (CGD) is an inherited immunodeficiency caused by defects in any of four genes encoding components of the leukocyte nicotinamide dinucleotide phosphate, reduced (NADPH) oxidase...
  80. ncbi Branched fungal beta-glucan causes hyperinflammation and necrosis in phagocyte NADPH oxidase-deficient mice
    Mg Schäppi
    Paediatric Gastroenterology and Transplantation Division, Department of Paediatrics, Geneva University Hospitals, 1211 Geneva 4, Switzerland
    J Pathol 214:434-44. 2008
    Chronic granulomatous disease (CGD), a genetic disorder characterized by the absence of a functional phagocyte NADPH oxidase, is a severe immune deficiency. However, non-infectious hyperinflammation is a second hallmark of the disease...
  81. ncbi Diagnosis and carrier detection of chronic granulomatous disease in five families by flow cytometry
    A D Crockard
    Regional Immunology Laboratory, Royal Victoria Hospital, UK
    Int Arch Allergy Immunol 114:144-52. 1997
    ..determination of phagocyte respiratory burst (ROB) activity, to the investigation of chronic granulomatous disease (CGD) may lead to improved laboratory detection of patients and carriers and indicate the nature of the molecular defect...
  82. pmc The p47(phox-/-) mouse model of chronic granulomatous disease has normal granuloma formation and cytokine responses to Mycobacterium avium and Schistosoma mansoni eggs
    B H Segal
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 67:1659-65. 1999
    Chronic granulomatous disease (CGD) is a genetic disorder of NADPH oxidase in which phagocytes are defective in generating reactive oxidants...
  83. ncbi Autosomal recessive chronic granulomatous disease caused by defects in NCF-1, the gene encoding the phagocyte p47-phox: mutations not arising in the NCF-1 pseudogenes
    D Noack
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 97:305-11. 2001
    Chronic granulomatous disease (CGD) is a primary immunodeficiency caused by defects in any one of 4 genes encoding phagocyte NADPH oxidase subunits...
  84. ncbi The search for a genetic defect in Polish patients with chronic granulomatous disease
    Monika Jurkowska
    Department of Medical Genetics, Institute of Mother and Child, Warsaw, Poland
    Arch Immunol Ther Exp (Warsz) 52:441-6. 2004
    ..Among the at least 5 subunits cre-ating a functional NADPH oxidase, a molecular defect located in any of the gp91phox, p22phox, p47phox, or p67phox subunits may cause CGD.
  85. pmc Hepatic involvement and portal hypertension predict mortality in chronic granulomatous disease
    Jordan J Feld
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 1800, USA
    Gastroenterology 134:1917-26. 2008
    Chronic granulomatous disease (CGD) is a rare genetic disorder, predisposing affected individuals to recurrent infectious complications and shortened survival...
  86. doi Ocular manifestations in chronic granulomatous disease in Saudi Arabia
    Saleh Al-Muhsen
    Department of Pediatrics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
    J AAPOS 13:396-9. 2009
    Chronic granulomatous disease (CGD) is a primary immunodeficiency disease caused by a genetic defect in the NADPH oxidase complex of phagocytic cells...
  87. pmc Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide and is fully maintained in chronic granulomatous disease
    Suk See De Ravin
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 116:1755-60. 2010
    In chronic granulomatous disease (CGD), defective phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity causes reduced superoxide anion (O(2)(·)) radical production leading to frequent infections as well as ..
  88. pmc Biochemical correction of X-CGD by a novel chimeric promoter regulating high levels of transgene expression in myeloid cells
    Giorgia Santilli
    Molecular Immunology Unit, Institute of Child Health, London, UK
    Mol Ther 19:122-32. 2011
    X-linked chronic granulomatous disease (X-CGD) is a primary immunodeficiency caused by mutations in the CYBB gene encoding the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase catalytic subunit gp91(phox)...
  89. pmc PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes
    S Suzuki
    Department of Biochemistry, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852, Japan
    Proc Natl Acad Sci U S A 95:6085-90. 1998
    ..1 to the promoter. We therefore conclude that PU.1 is an essential activator for the expression of gp91(phox) gene in human neutrophils, monocytes, and B lymphocytes...
  90. doi Toxicity of repeated intravenous injection of gene therapeutics for X-CGD in mice
    Y M Lee
    Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung Gu, Daejeon, Korea
    Hum Exp Toxicol 27:401-7. 2008
    We made gene therapeutics for X-chronic granulomatous disease (CGD) by transducing murine bone marrow-derived stem cells with MT-gp91 retrovirus and evaluated possible toxicity in mice as a prerequisite for human clinical trials...
  91. ncbi Pivotal role of a gp91(phox)-containing NADPH oxidase in angiotensin II-induced cardiac hypertrophy in mice
    Jennifer K Bendall
    Department of Cardiology, Guy s, King s and St Thomas School of Medicine, King s College London, London, UK
    Circulation 105:293-6. 2002
    ..Angiotensin II treatment increased myocardial NADPH oxidase activity in wild-type but not gp91(phox-/-) mice...
  92. ncbi Nox4 mediates angiotensin II-induced activation of Akt/protein kinase B in mesangial cells
    Yves Gorin
    Department of Medicine, The University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    Am J Physiol Renal Physiol 285:F219-29. 2003
    ..We explored the role of the small GTPase Rac1, a component of the phagocyte NADPH oxidase, and the gp91phox homologue Nox4/Renox in this signaling pathway...
  93. pmc Decreased neointimal formation in Nox2-deficient mice reveals a direct role for NADPH oxidase in the response to arterial injury
    Zhiping Chen
    Cardiovascular Division, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:13014-9. 2004
    ..We addressed this issue by subjecting leukocyte oxidase (Nox2)-deficient (Nox2-/-) mice to arterial injury...
  94. ncbi Successful low toxicity hematopoietic stem cell transplantation for high-risk adult chronic granulomatous disease patients
    Tayfun Gungor
    Division of Immunology Hematology BMT, University Children s Hospital, CH 8032 Zurich, Switzerland
    Transplantation 79:1596-606. 2005
    Allogeneic hematopoietic stem cell transplantation for chronic granulomatous disease (CGD) is associated with a significant risk of transplant-related mortality...
  95. ncbi Left ventricular remodeling after myocardial infarction in mice with targeted deletion of the NADPH oxidase subunit gp91PHOX
    Stefan Frantz
    Medizinische Universitätsklinik Würzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
    Basic Res Cardiol 101:127-32. 2006
    ..Since NADPH oxidases are a major source of reactive oxygen species in the heart, we studied left ventricular remodeling after myocardial infarction in mice with targeted deletion of the NADPH oxidase subunit gp91(phox)...
  96. pmc NO-mediated regulation of NAD(P)H oxidase by laminar shear stress in human endothelial cells
    Nicole Duerrschmidt
    University of Technology Dresden, Medical Faculty Carl Gustav Carus, Department of Vascular Endothelium and Microcirculation, Fetscherstr 74, D 01307 Dresden, Germany
    J Physiol 576:557-67. 2006
    ..O(2)(-) formation, and mRNA and protein expression of NAD(P)H oxidase subunits Nox2/gp91(phox) and p47(phox)...
  97. ncbi Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
    ..The recruitment of the NADPH oxidase NOX2 prevents acidification of phagosomes, limiting antigen degradation...
  98. pmc Restoration of NET formation by gene therapy in CGD controls aspergillosis
    Matteo Bianchi
    Division of Immunology Haematology BMT, University Children s Hospital Zurich, Zurich, Switzerland
    Blood 114:2619-22. 2009
    Chronic granulomatous disease (CGD) patients have impaired nicotinamide adenine dinucleotide phosphate (NADPH) oxidase function, resulting in poor antimicrobial activity of neutrophils, including the inability to generate neutrophil ..
  99. pmc Anti-thrombin therapy during warm ischemia and cold preservation prevents chronic kidney graft fibrosis in a DCD model
    F Favreau
    Inserm U927, Ischémie reperfusion en transplantation rénale, Poitiers, France
    Am J Transplant 10:30-9. 2010
    ..Finally, expression of oxidative stress markers Nox2 and iNOS was reduced...
  100. pmc Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease
    Felix Meissner
    Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin, Germany
    Blood 116:1570-3. 2010
    ..CGD, we analyzed cells isolated from patients with defects in the phagocyte oxidase subunits p22phox, p47phox or gp91phox. We report that mononuclear phagocytes from CGD patients activated caspase-1 and produced biologically active ..
  101. doi Nox isoforms in vascular pathophysiology: insights from transgenic and knockout mouse models
    Jennifer Rivera
    Department of Pharmacology, Monash University, Clayton, Victoria, Australia
    Redox Rep 15:50-63. 2010
    ..The NADPH oxidases are a family of ROS generating enzymes, of which four members (Nox1, Nox2, Nox4 and Nox5) are expressed in blood vessels...

Research Grants65

  1. Biosynthesis, structural organization, and function of NOX proteins
    WILLIAM NAUSEEF; Fiscal Year: 2007
    ..the essential membrane component of this NADPH oxidase, is a heterodimeric protein composed of p22p'hox and gp91 phox and previously thought to be a protein unique to phagocytes, although recent studies have identified a family of ..
  2. Redox regulation of phagocyte NOX2 in inflammation and aging
    Robert A Clark; Fiscal Year: 2013
    ..The phagocyte oxidase features membrane-bound catalytic components (gp91phox/p22phox) and cytosolic co-factors (p47phox, p67phox, Rac1/2) that undergo stimulus-dependent translocation during ..
  3. Taylor W Starnes; Fiscal Year: 2014
    ..CGD usually results from mutations in Nox2, the catalytic subunit of the ROS-producing NADPH oxidase complex in phagocytes...
  4. Macrophage PPARg signaling, efferocytosis, and exaggerated inflammation in CGD
    Donna L Bratton; Fiscal Year: 2011
    Chronic Granulomatous Disease (CGD) is a genetic disease resulting from mutation of the phagocyte NADPH oxidase...
  5. Function of NADPH Oxidase(s) in T Lymphocytes
    Mark S Williams; Fiscal Year: 2010
    ..The absence or mutation of components of this oxidase (NOX2/gp91phox, p47phox, p67phox, p22phox, Rac2) results in chronic granulomatous disease (CGD)...
  6. Novel Approach to Oral Gene Therapy for Chronic Granulomatous Disease
    Peter E Newburger; Fiscal Year: 2010
    ..X-linked CGD results from mutations in the CYBB gene encoding the cytochrome b heavy chain glycoprotein, gp91-phox...
  7. Role of NADPH Oxidase in Regulating Inflammation
    Brahm H Segal; Fiscal Year: 2013
    Chronic granulomatous disease (CGD) is a rare inherited disorder of the NADPH oxidase complex in which phagocytes are defective in generating superoxide anion and downstream reactive oxidant intermediates (ROIs)...
  8. The role of neutrophil extracellular traps and NADPH oxidase in SLE pathogenesis
    ALLISON MARIE CAMPBELL; Fiscal Year: 2012
    ..it has been observed that humans with an X-linked defect in NADPH oxidase and hence chronic granulomatous disease (CGD), whose neutrophils cannot form NETs have an increased predisposition to SLE...
  9. MOLECULAR MECHANISMS OF LEUKOCYTE ACTIVATION
    Richard Ye; Fiscal Year: 2009
    ..However, extracellular ROS production by phagocyte NADPH oxidase (Nox2) can be harmful to the tissue and is a major cause of vascular injury in acute inflammation...
  10. Richard D Ye; Fiscal Year: 2014
    ..However, extracellular ROS production by phagocyte NADPH oxidase (also termed Nox2) can be harmful to the tissue and is a major cause of vascular injury in acute inflammation...
  11. Keer Sun; Fiscal Year: 2016
    ..b>NADPH oxidase 2 (NOX2) is an enzyme complex predominantly expressed by phagocytes...
  12. Laura L Dugan; Fiscal Year: 2014
    ..activation of the transcription factor, NFkB, induces neuronal expression and activation of NADPH oxidase (Nox2), a multimeric enzyme complex first described as the respiratory burst oxidase in neutrophils...
  13. Small Molecule targeting of NADPH oxidase in neutrophils
    Marie Dominique Filippi; Fiscal Year: 2010
    ..the present Phase I STTR proposal focuses on utilizing our detailed understanding of the molecular regulation of NOX2 NADPH oxidase by Rac GTPases to develop first-in-class treatments for acute and chronic lung disorders...
  14. Role of NADPH oxidase in insulin resistance
    Amina El Jamali; Fiscal Year: 2013
    ..While the role of NOX2 expressed in neutrophils is well described in host defense and inflammation, little is known about the potential ..
  15. REGULATION OF SUPEROXIDE PRODUCTION BY HUMAN NEUTROPHILS
    Jeffrey Friedman; Fiscal Year: 2007
    ..damage results from the excessive production of phagocyte oxidants, while in chronic granulomatous disease (CGD), failure to produce these molecules leads to life-threatening infections...
  16. Samantha M Yeligar; Fiscal Year: 2014
    ..In alveolar macrophages (AMs), NADPH oxidase (Nox) 1, Nox2, and Nox4 are critical sources of reactive oxygen species (ROS), and Nox2 is essential for the respiratory burst ..
  17. Swapan K Nath; Fiscal Year: 2014
    ..This multi-protein complex is encoded by 7 essential genes: NCF1, NCF2, NCF4, CYBA,CYBB, Rac1 or Rac2...
  18. Non-oxidative Resistance to A. fumigatus by Alveolar Macrophages
    James B Burritt; Fiscal Year: 2010
    ..Three aims proposed to address this goal are: 1) to compare the conidiacidal activity of AM from normal and gp91phox-/- mice that lack a functional phagocyte oxidase, using both in vitro and in vivo analyses...
  19. Renu A Kowluru; Fiscal Year: 2015
    ..Our preliminary data show that hyperglycemia activates Rac1/Nox2 signaling axis in the retina and its capillary cells prior to mitochondrial dysregulation, suggesting that Nox2 ..
  20. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Celine DerMardirossian; Fiscal Year: 2010
    ..It has been established that the activity of the human neutrophil ROS-generating NADPH oxidase (Nox2) is regulated by the small GTPase Rac2, and that Rac2 acts as a key "molecular switch" for ROS formation in ..
  21. HOW DOES RAC CONTROL HUMAN PHAGOCYTE NADPH OXIDASE?
    Paul Heyworth; Fiscal Year: 1999
    ..The importance of NADPH oxidase is made very apparent by the inherited syndrome chronic granulomatous disease (CGD), in which oxidase activity is absent and affected individuals are susceptible to life-threatening infections...
  22. Marschall S Runge; Fiscal Year: 2015
    ..Although molecular mechanisms activating the Nox2 (gp91phox) NADPH oxidase have been elucidated, the events that lead to dysregulation of Nox1 NADPH oxidase activity are not ..
  23. Endothelial Generation of ROS in lung Ischemia
    Aron B Fisher; Fiscal Year: 2012
    ..is characterized by endothelial cell membrane depolarization, activation of cell membrane associated NADPH oxidase (NOX2), generation of reactive oxygen species (ROS), activation of transcription factors (NF(B, AP-1, and others), and ..
  24. Ruth B Caldwell; Fiscal Year: 2015
    ..The investigators have shown that activation of the superoxide generating NADPH oxidase enzyme NOX2 is a key feature of vascular inflammation during diabetes and other diseases characterized by retinal inflammatory ..
  25. Aron B Fisher; Fiscal Year: 2015
    ..by applicant): The goal of this proposal is to evaluate a novel role for Prdx6 in the activation of NADPH oxidase (NOX2)...
  26. Raghu Vemuganti; Fiscal Year: 2014
    ..We will test apocynin that inhibits NADPH oxidase subunit NOX2 and thus curtails reactive oxygen species (ROS) formation, and TBHQ that potentiates the transcription factor Nrf2 ..
  27. Redox Triggering of Glomerular NALP3 Inflammasomes in Hyperhomocysteinemia
    Justine M Abais; Fiscal Year: 2013
    ..glomerular injury are blocked by selective inhibition of local Nox activity and expression or by knocking out the gp91phox gene in mice...
  28. Pak H Chan; Fiscal Year: 2015
    ..MMP-9 activation, BBB damage, and neuronal death by an oxidative stress mechanism that may involve NADPH oxidase (NOX2), a major cytosolic superoxide radical-producing enzyme...
  29. Oxidative stress in a murine model of sleep apnea
    David Gozal; Fiscal Year: 2012
    ..of oxidative stress, and apoptosis as follows: (1) The effect of genetic absence of NADPH oxidase activity (gp91phox-/- mice) on the behavioral and neuronal susceptibility to 14 days of intermittent daytime hypoxia (IH);(2) The ..
  30. HTS for inhibitors of NADPH Oxidase 2 (NOX 2)
    Hugh Rosen; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Nox2 is one of the seven members of NOX/DUOX family of NADPH oxidases with the biological function of generating Reactive Oxygen Species (ROS)...
  31. OXIDATIVE STRESS AND ANTIOXIDANTS IN IRON OVERLOAD
    William Bernard Weglicki; Fiscal Year: 2010
    ..The involvement of NADPH oxidase during iron overload will also be assessed using gp91 phox knockout mice (Subcontract)...
  32. George G Rodney; Fiscal Year: 2016
    ..damaging effects of ROS. NADPH oxidase (Nox2) is an enzyme complex that generates ROS...
  33. Kimberly Byrnes; Fiscal Year: 2016
    ..assess inflammation after moderate SCI at acute, sub-acute and chronic time points using both genetic knockout (gp91PHOX) and pharmacological inhibition approaches...
  34. Ozone Primes Pulmonary Innate Immunity
    John W Hollingsworth; Fiscal Year: 2012
    ..leading to NF-?B activation and amplification of the airway inflammatory response in a manner dependent on NOX2 and ROS-production. The investigators will address this hypothesis through the following Specific Aims...
  35. X chromosome, injury and infection
    Zoltan Spolarics; Fiscal Year: 2012
    ..The study focuses on two X-linked proteins, the first, gp91phox (Cybb) is a critical protein component of the NADPH oxidase complex that produces superoxide anion required for ..
  36. Nox Isoforms and Vascular Cell Phenotype
    John Keaney; Fiscal Year: 2009
    ..others have found that endothelial cells contain two specific isoforms of the NADPH oxidase catalytic subunit (Nox);Nox2 and Nox4...
  37. Nox1 and Nox4 in Neuronal Apoptosis
    STEVEN TAMMARIELLO; Fiscal Year: 2007
    ..preliminary data suggesting Nox1 and Nox4 are the catalytic subunits of the neuronal NADPH oxidase, as opposed to Nox2 (gp91-phox) the subunit that is expressed in phagocytic cells...
  38. The Role of Hypothalamic Superoxide in the Pathogenesis of Diet-Induced Obesity
    Stephan J Guyenet; Fiscal Year: 2012
    ..oxygen radical superoxide, and a concomitant increase in the expression of the superoxide-generating enzyme NADPH oxidase 2 (NOX2)...
  39. Arjang Djamali; Fiscal Year: 2015
    ..Increasing evidence suggests that Nox2 is at the crossroads of CNI-induced renal hypoxia and fibrosis...
  40. Natalie J Torok; Fiscal Year: 2016
    ..Thus, our HYPOTHESIS is that NOX2 activation with superoxide production in HSC is a key event during liver fibrogenesis...
  41. Sigrid C Veasey; Fiscal Year: 2015
    ..Also, in the previous award period we identified NADPH oxidase (Nox2) as a key source of neuronal injury and apoptosis...
  42. Raghu Vemuganti; Fiscal Year: 2014
    ..stress connected? In particular, if ER stress mediated by PERK and oxidative stress modulated by NADPH oxidase NOX2 influence each other? (3) What is the effect of knocking-out/inhibiting individual rate-limiting proteins of PERK ..
  43. ICSBP Function During Myeloid Differentiation
    ELIZABETH ANN EKLUND; Fiscal Year: 2011
    ..For example, we found that ICSBP activates transcription of genes encoding the phagocyte NADPH-oxidase proteins, gp91PHOX and p67phox. Other investigators identified additional ICSBP-target-genes involved in phagocyte function...
  44. MOHAMED AL SAYED AL-SHABRAWEY; Fiscal Year: 2016
    ..The impact of intraocular injection of HETEs in NADPH oxidase catalytic subunit NOX2-deficient mice will be compared to the effect of HETEs in wild type mice...
  45. Age Impaired ER Homeostasis in Wake-Active Neurons: BiP/Nox2 Crosstalk
    Nirinjini Naidoo; Fiscal Year: 2009
    ..We hypothesize that an oxidase enzyme in wake neurons progressively disrupts the chaperoning system in the endoplasmic reticulum. Consequently protein homeostasis is compromised and toxic protein aggregates accumulate. ..
  46. Effect of HIV Tat on endothelial cell function
    Lance Terada; Fiscal Year: 2009
    ..Our preliminary data now reveal that Tat simultaneously activates both Nox2 and Nox4 pathways downstream of Rac1, leading to independent PAK/JNK and Ras/ERK activation...
  47. Generation and utility of NADPH oxidase inhibitors
    Sara A Courtneidge; Fiscal Year: 2012
    ..The human genome contains several NADPH oxidase catalytic subunits, called Nox1, Nox2, Nox3, Nox4 and Nox5...
  48. CHRISTOPHER WILLIAM WARD; Fiscal Year: 2014
    ..With this approach, we have discovered that in the mdx, stretch induced a burst of ROS release from NADPH Oxidase 2 (NOX2) a pathway termed X-ROS signaling...
  49. Jae U Jung; Fiscal Year: 2015
    ..and suppresses autophagosome maturation step under normal conditions, it is rapidly recruited to the p22phox-gp91phox NADPH oxidase complex upon microbial infection, facilitating the stabilization and phagosomal translocation of ..
  50. REG OF THE NADPH OXIDASE BY ANTI-INFLAMMATORY AGENTS
    Peter Newburger; Fiscal Year: 2001
    ..Better understanding of the pharmacological regulation of the NADPH oxidase system will provide information to help modulate its expression during inflammation and oxidant-mediated tissue damage. ..
  51. Inhibition of NADPH oxidase by Anaplasma phagocytophila
    JASON CARLYON; Fiscal Year: 2007
    ..It is established that the bacterium inhibits the mRNA expression of gp91phox, a component of NADPH oxidase. Preliminary data presented here demonstrates that A...
  52. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2003
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  53. Nox 4 in Endothelial Cell ROS Production, Signaling and Motility
    Viswanathan Natarajan; Fiscal Year: 2009
    ..oxidase, similar to phagocytic oxidase, consists of cytosolic (p47phox, p67phox and Rac1) and membranebound (gp91phox and p22phox) components...
  54. NOX1 and NOX2 as Therapeutic Targets in Influenza
    JOHN DAVID LAMBETH; Fiscal Year: 2013
    ..We propose a signaling cascade involving both epithelial NOX1- and monocyte/PMN Nox2-generated ROS as key steps that: a) facilitate viral replication and/or spread and b) mediate lung damage...
  55. Defective PS Exposure in Neutrophil Apoptosis in CGD
    Donna Bratton; Fiscal Year: 2007
    Chronic granulomatous disease (CGD) is a rare, inherited disorder of various defects of the NADPH oxidase rendering it dysfunctional and predisposing to invasive and life-threatening bacterial and fungal infection...
  56. Microbial Response to Neutrophil Phagocytosis
    Henry Rosen; Fiscal Year: 2006
    ..The effects of neutrophils deficient in antimicrobial systems mediated by the phagocyte NADPH oxidase (CGD, chronic granulomatous disease) or the enzyme myeloperoxidase will be compared with normal neutrophils...
  57. Junichi Sadoshima; Fiscal Year: 2016
    ..Although the cellular function of plasma-membrane associated Noxs, such as Nox1 and p91phox (Nox2), has been extensively characterized, that of Nox4, an isoform of Nox expressed in the heart, is not well ..
  58. Morton Cowan; Fiscal Year: 2016
    ..with Severe Combined Immunodeficiency (SCID), Wiskott-Aldrich Syndrome (WAS) and Chronic Granulomatous Disease (CGD) who receive HCT or other forms of treatment...
  59. Morton Cowan; Fiscal Year: 2016
    ..therapy: severe combined immunodeficiency (SCID), Wiskott-Aldrich syndrome (WAS) and chronic granulomatous disease (CGD)...
  60. Joanna B Goldberg; Fiscal Year: 2014
    ..in two seemingly disparate hosts: those with cystic fibrosis (CF) and those with chronic granulomatous disease (CGD)...
  61. JOHN DAVID LAMBETH; Fiscal Year: 2014
    ..modes of regulation: 1) constitutively active (Nox4);2) activation by assembly with regulatory subunits (Nox1, Nox2 and Nox3);and 3) calcium-activated (Nox5, Duox1 and Duox2)...
  62. Regulation of Nox Enzymes by Calcium and Novel Subunits
    J Lambeth; Fiscal Year: 2009
    ..modes of regulation: 1) constitutively active (Nox4);2) activation by assembly with regulatory subunits (Nox1, Nox2 and Nox3);and 3) calcium-activated (Nox5, Duox1 and Duox2)...
  63. WHITE BLOOD CELL OXIDASE IN LEUKEMIA AND NORMAL CELLS
    Peter Newburger; Fiscal Year: 1991
    ..of the HL-60 human promelocytic leukemia cell line and in various forms of chronic granulomatous disease (CGD). During the next five years, the proposed studies will extend to the molecular level of regulation of the oxidase...
  64. ENDOTHELIAL DYSFUNCTION AND A LOW SALT DIET
    Thomas Hintze; Fiscal Year: 2006
    ..Aim 2 will use the gp91phox KO -/- and p47 -/- mouse heart to further elucidate the relationship between angiotensin II, the NADPH oxidase ..
  65. Role of NADPH Oxidase in ICH-Induced Brain Injury
    Jiping Tang; Fiscal Year: 2006
    ..This hypothesis is derived from our preliminary observations that gp91phox subunit of NADPH oxidase in the ipsilateral hemisphere is upregulated at the transcriptional level after ICH, ..