Dicer

Summary

Gene Symbol: Dicer
Description: dicer 1, ribonuclease III
Alias: DCR1, Dicer, Dicer1e, HERNA, K12H4.8-LIKE, MNG1, RMSE2, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
Species: human

Top Publications

  1. pmc Autoinhibition of human dicer by its internal helicase domain
    Enbo Ma
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3206, USA
    J Mol Biol 380:237-43. 2008
  2. pmc Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma
    Jie Lin
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Carcinogenesis 31:1805-12. 2010
  3. pmc The loop position of shRNAs and pre-miRNAs is critical for the accuracy of dicer processing in vivo
    Shuo Gu
    Departments of Pediatrics and Genetics, Stanford University, Stanford, CA 94305, USA
    Cell 151:900-11. 2012
  4. pmc Dicer recognizes the 5' end of RNA for efficient and accurate processing
    Jong Eun Park
    School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 475:201-5. 2011
  5. pmc TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 436:740-4. 2005
  6. pmc Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype
    G Grelier
    Universite de Lyon, Universite Lyon 1, ISPB, Lyon F 69003, France
    Br J Cancer 101:673-83. 2009
  7. pmc Ribonuclease activity and RNA binding of recombinant human Dicer
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
    EMBO J 21:5864-74. 2002
  8. doi Biallelic DICER1 mutations occur in Wilms tumours
    M K Wu
    Department of Medical Genetics, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, Canada
    J Pathol 230:154-64. 2013
  9. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
  10. pmc DICER1 expression and outcomes in endometrioid endometrial adenocarcinoma
    Israel Zighelboim
    Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Washington University School of Medicine and Siteman Cancer Center, St Louis, MO 63110, USA
    Cancer 117:1446-53. 2011

Research Grants

  1. Ribonuclease III mechanism of double-strand RNA cleavage
    ALLEN NICHOLSON; Fiscal Year: 2003
  2. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
  3. Eric C Lai; Fiscal Year: 2016
  4. Scott A Showalter; Fiscal Year: 2015
  5. miRNAs and epidermal langerhans cell development and function
    Qing Sheng Mi; Fiscal Year: 2011
  6. Wei Yan; Fiscal Year: 2014
  7. Phillip D Zamore; Fiscal Year: 2014
  8. Daniel Cifuentes; Fiscal Year: 2014
  9. Phillip A Sharp; Fiscal Year: 2016
  10. Antonio J Giraldez; Fiscal Year: 2015

Detail Information

Publications222 found, 100 shown here

  1. pmc Autoinhibition of human dicer by its internal helicase domain
    Enbo Ma
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3206, USA
    J Mol Biol 380:237-43. 2008
    b>Dicer, a member of the ribonuclease III family of enzymes, processes double-stranded RNA substrates into approximately 21- to 27-nt products that trigger sequence-directed gene silencing by RNA interference...
  2. pmc Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma
    Jie Lin
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Carcinogenesis 31:1805-12. 2010
    ..Haplotypes of DICER and DROSHA were also associated with altered patient survival and recurrence...
  3. pmc The loop position of shRNAs and pre-miRNAs is critical for the accuracy of dicer processing in vivo
    Shuo Gu
    Departments of Pediatrics and Genetics, Stanford University, Stanford, CA 94305, USA
    Cell 151:900-11. 2012
    Short hairpin RNA (shRNA)-induced RNAi is used for biological discovery and therapeutics. Dicer, whose normal role is to liberate endogenous miRNAs from their precursors, processes shRNAs into different biologically active siRNAs, ..
  4. pmc Dicer recognizes the 5' end of RNA for efficient and accurate processing
    Jong Eun Park
    School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 475:201-5. 2011
    ..silencing is a class of approximately 22-nucleotide RNAs that are processed from double-stranded RNA precursors by Dicer. Accurate processing by Dicer is crucial for the functionality of microRNAs (miRNAs)...
  5. pmc TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 436:740-4. 2005
    ..These pre-miRNAs are cleaved by the RNase III Dicer to generate mature miRNAs that direct the RNA-induced silencing complex (RISC) to messenger RNAs with ..
  6. pmc Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype
    G Grelier
    Universite de Lyon, Universite Lyon 1, ISPB, Lyon F 69003, France
    Br J Cancer 101:673-83. 2009
    b>Dicer, a ribonuclease, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs and is essential for both mammalian development and cell differentiation...
  7. pmc Ribonuclease activity and RNA binding of recombinant human Dicer
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
    EMBO J 21:5864-74. 2002
    ..RNAi) in animals, are mediated by double-stranded RNA (dsRNA) and mechanistically intersect at the ribonuclease Dicer. Here, we report cloning and expression of the 218 kDa human Dicer, and characterization of its ribonuclease ..
  8. doi Biallelic DICER1 mutations occur in Wilms tumours
    M K Wu
    Department of Medical Genetics, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, Canada
    J Pathol 230:154-64. 2013
    ..This study has demonstrated that a subset of WTs exhibits two 'hits' in DICER1, suggesting that these mutations could be key events in the pathogenesis of these tumours...
  9. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
    ....
  10. pmc DICER1 expression and outcomes in endometrioid endometrial adenocarcinoma
    Israel Zighelboim
    Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Washington University School of Medicine and Siteman Cancer Center, St Louis, MO 63110, USA
    Cancer 117:1446-53. 2011
    ..The authors also explored DNA methylation and haploinsufficiency as potential mechanisms related to altered DICER1 expression in these tumors...
  11. doi Biallelic DICER1 mutations in sporadic pleuropulmonary blastoma
    Masafumi Seki
    Authors Affiliations Department of Pediatrics Cancer Genomics Project, Graduate School of Medicine Laboratory of DNA Information Analysis and Sequence Data Analysis, Human Genome Center, Institute of Medical Science Department of Cell Therapy and Transplantation Medicine, The University of Tokyo, Tokyo Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto Division of Pediatric Hematology and Oncology, Ibaraki Children s Hospital, Mito, Ibaraki Department of Hematology Oncology, Saitama Children s Medical Center, Saitama, Saitama Department of Pediatrics, School of Medicine, Fukuoka University, Fukuoka Gunma Children s Medical Center, Shibukawa, Gunma Department of Hematology and Oncology, Hyogo Prefectural Kobe Children s Hospital, Kobe, Hyogo and National Center for Child Health and Development, Tokyo, Japan
    Cancer Res 74:2742-9. 2014
    ..Our results highlight the role of RNase IIIb domain mutations in DICER1 along with TP53 inactivation in PPB pathogenesis...
  12. pmc Dicer, Drosha, and outcomes in patients with ovarian cancer
    William M Merritt
    University of Texas M D Anderson Cancer Center, Houston, TX 77230, USA
    N Engl J Med 359:2641-50. 2008
    We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.
  13. pmc DICER1 mutations in familial pleuropulmonary blastoma
    D Ashley Hill
    Washington University Medical Center, St Louis, MO 63110, USA
    Science 325:965. 2009
    ..We hypothesize that loss of DICER1 in the epithelium of the developing lung alters the regulation of diffusible factors that promote mesenchymal proliferation...
  14. pmc Coordinated activities of human dicer domains in regulatory RNA processing
    Enbo Ma
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Mol Biol 422:466-76. 2012
    The conserved ribonuclease Dicer generates microRNAs and short-interfering RNAs that guide gene silencing in eukaryotes...
  15. pmc Phosphorylation of the human microRNA-generating complex mediates MAPK/Erk signaling
    Zain Paroo
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 139:112-22. 2009
    ..We determined that the human miRNA-generating complex is comprised of Dicer and phospho-TRBP isoforms. Phosphorylation of TRBP is mediated by the mitogen-activated protein kinase (MAPK) Erk...
  16. pmc Genetic variation in MicroRNA genes and risk of oral premalignant lesions
    Jessica Clague
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Carcinog 49:183-9. 2010
    ..09; 95% CI, 1.23-3.56). Likewise, patients with at least one variant allele of DICER:rs3742330 had a significantly increased risk of OPL (OR, 2.09; 95% CI, 1.03-4.24)...
  17. ncbi Dicer is essential for formation of the heterochromatin structure in vertebrate cells
    Tatsuo Fukagawa
    Precursory Research for Embryonic Science and Technology of Japan Science and Technology Agency, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 6:784-91. 2004
    RNA interference is an evolutionarily conserved gene-silencing pathway in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs...
  18. pmc The HIV-1 protein Vpr targets the endoribonuclease Dicer for proteasomal degradation to boost macrophage infection
    Laurieann Casey Klockow
    Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208, USA
    Virology 444:191-202. 2013
    ..Here we identify the endoribonuclease Dicer as a target of HIV-1 Vpr-directed proteasomal degradation through CRL4(DCAF1)...
  19. pmc Expanding the phenotype of mutations in DICER1: mosaic missense mutations in the RNase IIIb domain of DICER1 cause GLOW syndrome
    Steven Klein
    Department of Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Med Genet 51:294-302. 2014
    ..Here we report a novel syndrome termed GLOW, an acronym for its core phenotypic findings, which include Global developmental delay, Lung cysts, Overgrowth and Wilms tumour caused by mutations in the RNase IIIb domain of DICER1...
  20. pmc Decreased Dicer expression elicits DNA damage and up-regulation of MICA and MICB
    Kai Fu Tang
    Key Laboratory of Molecular Biology for Infectious Diseases of the State Ministry of Education, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People s Republic of China
    J Cell Biol 182:233-9. 2008
    ..RNAi) acts constitutively to silence the innate immune response, and innate immunity genes are misregulated in Dicer-deficient Caenorhabditis elegans...
  21. pmc microRNA signature and expression of Dicer and Drosha can predict prognosis and delineate risk groups in neuroblastoma
    Ruey Jen Lin
    Graduate Institute of Life Science, National Defense Medical Center, and Genomics Research Center, Academia Sinica, Taipei, Taiwan
    Cancer Res 70:7841-50. 2010
    ..To investigate the microRNA (miRNA) profile and role of Dicer and Drosha in neuroblastoma, we assessed the expression of 162 human miRNAs, Dicer and Drosha in 66 neuroblastoma ..
  22. pmc Two-step cleavage of hairpin RNA with 5' overhangs by human DICER
    Yoshinari Ando
    RIKEN Omics Science Center, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama 230 0045, Japan
    BMC Mol Biol 12:6. 2011
    b>DICER is an RNase III family endoribonuclease that processes precursor microRNAs (pre-miRNAs) and long double-stranded RNAs, generating microRNA (miRNA) duplexes and short interfering RNA duplexes with 20~23 nucleotides (nts) in length...
  23. doi Dicer 1, ribonuclease type III modulates a reprogramming effect in colorectal cancer cells
    Dyah Laksmi Dewi
    Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Int J Mol Med 29:1060-4. 2012
    ..factors into two distinct colorectal cancer (CRC) cell lines, HCT116 and DLD-1, in the presence and absence of Dicer 1, ribonuclease type III (Dicer1), a critical miRNA processing enzyme...
  24. pmc Dicer-mediated upregulation of BCRP confers tamoxifen resistance in human breast cancer cells
    Jennifer Selever
    Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, Texas, USA
    Clin Cancer Res 17:6510-21. 2011
    ..Using microarray analysis, we observed that metastatic breast tumors resistant to Tam therapy had elevated levels of Dicer.
  25. doi High expression of Dicer reveals a negative prognostic influence in certain subtypes of primary cutaneous T cell lymphomas
    Julia Valencak
    Department of Dermatology, Medical University of Vienna, Austria
    J Dermatol Sci 64:185-90. 2011
    Aberrant expression of microRNAs (miRNAs) has been implicated in oncogenesis of various tumors and primary cutaneous T cell lymphomas. Dicer, a ribonuclease III-like enzyme is essential for miRNA processing.
  26. ncbi Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA
    Kin Hang Kok
    Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China
    J Biol Chem 282:17649-57. 2007
    Mammalian Dicer interacts with double-stranded RNA-binding protein TRBP or PACT to mediate RNA interference and micro-RNA processing. TRBP and PACT are structurally related but exert opposite regulatory activities on PKR...
  27. pmc MicroRNAs link estrogen receptor alpha status and Dicer levels in breast cancer
    Dawn R Cochrane
    Department of Pathology, University of Colorado Denver, Mail Stop 8104, P O Box 6511, Aurora, CO 80045, USA
    Horm Cancer 1:306-19. 2010
    ..Addition of these miRNAs to ESR1+ cells reduces Dicer protein, whereas antagonizing miR-222 in ESR1- cells increases Dicer protein...
  28. pmc A human, ATP-independent, RISC assembly machine fueled by pre-miRNA
    Elisavet Maniataki
    Department of Pathology, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Genes Dev 19:2979-90. 2005
    ..The Dicer nuclease produces mature miRNAs and siRNAs from pre-miRNAs and double-stranded RNA (dsRNA), respectively, and ..
  29. pmc HIV-1 TAR element is processed by Dicer to yield a viral micro-RNA involved in chromatin remodeling of the viral LTR
    Zachary Klase
    Immunology, Microbiology and Tropical Medicine program, The George Washington University School of Medicine, Washington, District of Columbia 20037, USA
    BMC Mol Biol 8:63. 2007
    ..There is growing evidence that many viruses have developed in the context of RNAi and express either a suppressor of RNAi or their own viral miRNA...
  30. pmc In vitro reconstitution of the human RISC-loading complex
    Ian J Macrae
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:512-7. 2008
    ..by the action of a specialized assembly called the RISC-loading complex (RLC), comprising the proteins Ago2, Dicer, and TRBP. Here we show that the human RLC assembles spontaneously in vitro from purified components...
  31. doi let-7 regulates Dicer expression and constitutes a negative feedback loop
    Shogo Tokumaru
    Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    Carcinogenesis 29:2073-7. 2008
    microRNAs (miRNA) are small, endogenously expressed non-coding RNAs that are sequentially processed by Drosha and Dicer from primary transcripts, by negatively regulating the expression of protein-coding genes through either ..
  32. pmc Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma
    Yohei Horikawa
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 14:7956-62. 2008
    ..Compelling evidence has shown that miRNAs are involved in cancer initiation and progression. We hypothesized that genetic variations of the miRNA machinery genes could be associated with the risk of renal cell carcinoma...
  33. pmc Dicer is regulated by cellular stresses and interferons
    Jennifer L Wiesen
    Roswell Park Cancer Institute, Laboratory of Molecular Medicine, Department of Immunology, Elm and Carlton Streets, Buffalo, NY 14263, United States
    Mol Immunol 46:1222-8. 2009
    The generation of microRNAs is dependent on the RNase III enzyme Dicer, the levels of which vary in different normal cells and in disease states...
  34. pmc Dicer-regulated microRNAs 222 and 339 promote resistance of cancer cells to cytotoxic T-lymphocytes by down-regulation of ICAM-1
    Ryo Ueda
    Department of Neurological Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Proc Natl Acad Sci U S A 106:10746-51. 2009
    The RNase III endonuclease Dicer plays a key role in generation of microRNAs (miRs). We hypothesized that Dicer regulates cancer cell susceptibility to immune surveillance through miR processing...
  35. pmc Substrate-specific kinetics of Dicer-catalyzed RNA processing
    Srinivas Chakravarthy
    Howard Hughes Medical Institute, Berkeley, CA 94720, USA
    J Mol Biol 404:392-402. 2010
    The specialized ribonuclease Dicer plays a central role in eukaryotic gene expression by producing small regulatory RNAs-microRNAs (miRNAs) and short interfering RNAs (siRNAs)-from larger double-stranded RNA (dsRNA) substrates...
  36. pmc DICER1 mutations in familial multinodular goiter with and without ovarian Sertoli-Leydig cell tumors
    Thomas Rio Frio
    Program in Cancer Genetics, Department of Oncology, McGill University Health Centre, Montreal, Quebec, Canada
    JAMA 305:68-77. 2011
    ..Germline mutations in DICER1, a gene that codes for an RNase III endoribonuclease, have been identified in families affected by pleuropulmonary blastoma (PPB), some of whom include cases of MNG and gonadal tumors such as SLCTs...
  37. pmc The role of Dicer protein partners in the processing of microRNA precursors
    Edyta Koscianska
    Laboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
    PLoS ONE 6:e28548. 2011
    One of the cellular functions of the ribonuclease Dicer is to process microRNA precursors (pre-miRNAs) into mature microRNAs (miRNAs). Human Dicer performs this function in cooperation with its protein partners, AGO2, PACT and TRBP...
  38. ncbi Dicer dependent microRNAs regulate gene expression and functions in human endothelial cells
    Yajaira Suarez
    Department of Pathology, Yale University School of Medicine, New Haven, Conn 06536, USA
    Circ Res 100:1164-73. 2007
    b>Dicer is a key enzyme involved in the maturation of microRNAS (miRNAs). miRNAs have been shown to be regulators of gene expression participating in the control of a wide range of physiological pathways...
  39. doi Interaction of human T-cell lymphotropic virus type I Rex protein with Dicer suppresses RNAi silencing
    Makoto Abe
    Department of Life and Environmental Sciences, Chiba Institute of Technology, Narashino, Chiba, Japan
    FEBS Lett 584:4313-8. 2010
    ..A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs)...
  40. doi Dysregulated expression of dicer and drosha in breast cancer
    Min Yan
    Department of Oncology, First Affiliated Hospital of Zhengzhou University, No 1, East Jianshe Rd, Zhengzhou, 450003, People s Republic of China
    Pathol Oncol Res 18:343-8. 2012
    ..Altered expression of Dicer and Drosha, two key enzymes in the miRNA maturation, is believed to be one of the most important mechanisms...
  41. pmc Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer
    Nasser Tahbaz
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    EMBO Rep 5:189-94. 2004
    PAZ PIWI domain (PPD) proteins, together with the RNA cleavage products of Dicer, form ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs)...
  42. ncbi Upregulation of PHLDA2 in Dicer knockdown HEK293 cells
    Kai Fu Tang
    Key Laboratory of Molecular Biology for Infectious Diseases of State Ministry of Education, The Second Affiliated Hospital, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, PR China
    Biochim Biophys Acta 1770:820-5. 2007
    ..To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of ..
  43. pmc Dicer is associated with ribosomal DNA chromatin in mammalian cells
    Lasse Sinkkonen
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    PLoS ONE 5:e12175. 2010
    ..Components of the RNA silencing machinery are involved in different aspects of chromatin function in numerous organisms. However, association of RNA silencing with chromatin in mammalian cells remains unclear...
  44. ncbi Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase
    S Matsuda
    Department of Molecular Pathogenesis Nagoya University School of Medicine, Japan
    Biochim Biophys Acta 1490:163-9. 2000
    ..8, it might be a human homolog of the K12H4.8. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 14q31 near the marker D14S605...
  45. ncbi Extensive variation in the 5'-UTR of Dicer mRNAs influences translational efficiency
    Sundeep Singh
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ont, Canada
    Biochem Biophys Res Commun 335:643-50. 2005
    The Dicer enzyme is a key component of the RNA interference pathway and also responsible for the processing of micro RNAs, non-coding RNA molecules which regulate the activity of mRNAs by antisense base pairing...
  46. ncbi Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors
    Noboru Doi
    Department of Biophysics, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, 113 0033, Bunkyo ku, Tokyo, Japan
    Curr Biol 13:41-6. 2003
    ..In lower eukaryotes, dsRNA introduced into the cytoplasm is cleaved by the RNaseIII-like enzyme, Dicer, to 21-23 nt RNA (short interfering [si] RNA), which may serve as guide for target mRNA degradation...
  47. pmc HIV-1 Tat interaction with Dicer: requirement for RNA
    Yamina Bennasser
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    Retrovirology 3:95. 2006
    b>Dicer is an RNase III which processes two classes of cellular small RNAs: the microRNAs (miRNA) and short interfering RNAs (siRNA)...
  48. pmc Small interfering RNAs against the TAR RNA binding protein, TRBP, a Dicer cofactor, inhibit human immunodeficiency virus type 1 long terminal repeat expression and viral production
    Helen S Christensen
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    J Virol 81:5121-31. 2007
    ..The mechanism uses the RNA-induced silencing complex, in which Dicer, Ago2, and the human immunodeficiency virus type 1 (HIV-1) TAR RNA binding protein (TRBP) are the main components...
  49. doi HCV core protein interacts with Dicer to antagonize RNA silencing
    Weixian Chen
    The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of the Ministry of Education, Chongqing Medical University, Chongqing, 400016, China
    Virus Res 133:250-8. 2008
    ..We have further demonstrated that HCV core protein directly interacts with Dicer, an RNase enzyme that generates siRNA in host cells...
  50. ncbi The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans
    Hiroaki Tabara
    Program in Molecular Medicine, University of Massachusetts Meidcal School, Worcester, MA 1605, USA
    Cell 109:861-71. 2002
    ..At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs)...
  51. pmc Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    EMBO J 21:5875-85. 2002
    b>Dicer is a multi-domain RNase III-related endonuclease responsible for processing double-stranded RNA (dsRNA) to small interfering RNAs (siRNAs) during a process of RNA interference (RNAi)...
  52. ncbi Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
    b>Dicer is a multidomain ribonuclease that processes double-stranded RNAs (dsRNAs) to 21 nt small interfering RNAs (siRNAs) during RNA interference, and excises microRNAs from precursor hairpins...
  53. pmc The contributions of dsRNA structure to Dicer specificity and efficiency
    Annaleen Vermeulen
    Dharmacon Inc, 2650 Crescent Dr, Suite 100, Lafayette, CO 80026, USA
    RNA 11:674-82. 2005
    b>Dicer processes long double-stranded RNA (dsRNA) and pre-microRNAs to generate the functional intermediates (short interfering RNAs and microRNAs) of the RNA interference pathway...
  54. pmc Alternative initiation and splicing in dicer gene expression in human breast cells
    Charletha V Irvin-Wilson
    Division of Cancer Biology, Department of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208, USA
    Breast Cancer Res 7:R563-9. 2005
    b>Dicer is a ribonuclease that mediates RNA interference both at the transcriptional and the post-transcriptional levels. Human dicer gene expression is regulated in different tissues...
  55. pmc TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing
    Astrid D Haase
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4508 Basel, Switzerland
    EMBO Rep 6:961-7. 2005
    b>Dicer is a key enzyme involved in RNA interference (RNAi) and microRNA (miRNA) pathways. It is required for biogenesis of miRNAs and small interfering RNAs (siRNAs), and also has a role in the effector steps of RNA silencing...
  56. pmc The role of PACT in the RNA silencing pathway
    Yoontae Lee
    Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul, Korea
    EMBO J 25:522-32. 2006
    ..In humans, small RNAs are generated by Dicer and assembled into the effector complex known as RNA-induced silencing complex (RISC) by multiple factors ..
  57. pmc Up-regulation of dicer, a component of the MicroRNA machinery, in prostate adenocarcinoma
    Simion Chiosea
    Department of Pathology, University of Pittsburgh, University of Pittsburgh Medical Center Presbyterian, C920 1, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Pathol 169:1812-20. 2006
    ..Production and function of microRNA requires coordinated processing by proteins of the microRNA machinery. Dicer, an RNase III endonuclease, is an essential component of the microRNA machinery...
  58. ncbi Overexpression of Dicer in precursor lesions of lung adenocarcinoma
    Simion Chiosea
    Department of Pathology, University of Pittsburgh Medical Center Presbyterian, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Cancer Res 67:2345-50. 2007
    ..The gene array analysis of BAC and adenocarcinoma showed a transient up-regulation of Dicer (a key effector protein for small interfering RNA and miR function) and PACT along with down-regulation of most ..
  59. pmc Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells
    Julia Höck
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    EMBO Rep 8:1052-60. 2007
    ..messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities...
  60. ncbi Decreased expression of DICER1 in gastric cancer
    Zhi hong Zheng
    Department of Laboratory Animal Science, China Medical University, Shenyang, China
    Chin Med J (Engl) 120:2099-104. 2007
    ..The aim of this study was to assess the correlation between the expression of DICER1, an epigenetically regulated gene, and gastric cancer...
  61. doi Mucoepidermoid carcinoma of upper aerodigestive tract: clinicopathologic study of 78 cases with immunohistochemical analysis of Dicer expression
    S I Chiosea
    Pathology and Laboratory Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    Virchows Arch 452:629-35. 2008
    ..miR maturation requires functional microRNA machinery, including the Dicer protein...
  62. pmc A role for the Dicer helicase domain in the processing of thermodynamically unstable hairpin RNAs
    Harris S Soifer
    Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Nucleic Acids Res 36:6511-22. 2008
    In humans a single species of the RNAseIII enzyme Dicer processes both microRNA precursors into miRNAs and long double-stranded RNAs into small interfering RNAs (siRNAs)...
  63. doi Downregulation of Dicer expression by serum withdrawal sensitizes human endothelial cells to apoptosis
    Satoshi Asada
    Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, and Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, 465 Kajii cho Kawaramachi Hirokoji, Kamigyo ku, Kyoto 602 8566, Japan
    Am J Physiol Heart Circ Physiol 295:H2512-21. 2008
    Although the modulated expression of Dicer is documented upon neoplastic transformation, little is known of the regulation of Dicer expression by environmental stimuli and its roles in the regulation of cellular functions in primary ..
  64. doi Human Dicer binds short single-strand and double-strand RNA with high affinity and interacts with different regions of the nucleic acids
    Walt F Lima
    Department of Molecular and Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USA
    J Biol Chem 284:2535-48. 2009
    Human Dicer is an integral component of the RNA interference pathway...
  65. ncbi Knockdown of Dicer in MCF-7 human breast carcinoma cells results in G1 arrest and increased sensitivity to cisplatin
    Ye Bu
    Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, PR China
    Oncol Rep 21:13-7. 2009
    Aberrant expression of microRNAs (miRNAs) in various human cancers suggests a role for miRNAs in tumorigenesis. Dicer is an essential component of the miRNA machinery, which mediates the processing of miRNAs...
  66. pmc A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function
    Sonia A Melo
    Cancer Epigenetics Laboratory, Spanish National Cancer Research Centre CNIO, 28029 Madrid, Spain
    Nat Genet 41:365-70. 2009
    ..Most important, the TRBP impairment is associated with a destabilization of the DICER1 protein. These results provide, for a subset of human tumors, an explanation for the observed defects in the expression of mature miRNAs...
  67. pmc Characterization of the TRBP domain required for dicer interaction and function in RNA interference
    Sylvanne M Daniels
    Virus Cell Interactions Laboratory, Lady Davis Institute for Medical Research, Montreal, Quebec, Canada
    BMC Mol Biol 10:38. 2009
    b>Dicer, Ago2 and TRBP are the minimum components of the human RNA-induced silencing complex (RISC). While Dicer and Ago2 are RNases, TRBP is the double-stranded RNA binding protein (dsRBP) that loads small interfering RNA into the RISC...
  68. pmc An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA
    Yoshiko Maida
    Cancer Stem Cell Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Nature 461:230-5. 2009
    ..polymerase (RdRP) activity and produces double-stranded RNAs that can be processed into small interfering RNA in a Dicer (also known as DICER1)-dependent manner...
  69. doi Down-regulation of dicer expression in ovarian cancer tissues
    Georgios Pampalakis
    Department of Pharmacy, University of Patras, Rion Patras, 26500, Greece
    Clin Biochem 43:324-7. 2010
    ..The purpose of this study was to investigate whether Dicer mRNA expression is altered during progression of ovarian cancer.
  70. pmc Structure of the human Dicer-TRBP complex by electron microscopy
    Pick Wei Lau
    National Resource for Automated Molecular Microscopy, The Scripps Institute, La Jolla, CA 92037, USA
    Structure 17:1326-32. 2009
    b>Dicer is a specialized ribonuclease that initiates RNA interference (RNAi) by cleaving double-stranded RNA (dsRNA) into small RNA fragments about 22 nucleotides long...
  71. doi Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1
    Kai Fu Tang
    Key Laboratory of Biorheological Science and Technology Chongqing University, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China
    Biochim Biophys Acta 1800:380-4. 2010
    b>Dicer is a multidomain ribonuclease III enzyme involved in the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs); depletion of Dicer was found to impair the migration of endothelial cells.
  72. pmc Overexpression of dicer as a result of reduced let-7 MicroRNA levels contributes to increased cell proliferation of oral cancer cells
    Andrew Jakymiw
    Department of Oral Biology, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610 0424, USA
    Genes Chromosomes Cancer 49:549-59. 2010
    Recent reports have demonstrated that Dicer, an RNase III endonuclease required for microRNA (miRNA) maturation, is aberrantly expressed in different types of cancer...
  73. doi Dicer in Schwann cells is required for myelination and axonal integrity
    Jorge A Pereira
    Institute of Cell Biology, Department of Biology, Eidgenossische Technische Hochschule ETH Zurich, CH 8093 Zurich, Switzerland
    J Neurosci 30:6763-75. 2010
    b>Dicer is responsible for the generation of mature micro-RNAs (miRNAs) and loading them into RNA-induced silencing complex (RISC). RISC functions as a probe that targets mRNAs leading to translational suppression and mRNA degradation...
  74. pmc Lineage-specific transcriptional regulation of DICER by MITF in melanocytes
    Carmit Levy
    Department of Dermatology, Cutaneous Biology Research Center, Mass General Hospital, Harvard Medical School, MA 02115, USA
    Cell 141:994-1005. 2010
    b>DICER is a central regulator of microRNA maturation. However, little is known about mechanisms regulating its expression in development or disease...
  75. pmc Absence of DICER in monocytes and its regulation by HIV-1
    William Coley
    National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University, Manassas, Virginia 20110, USA
    J Biol Chem 285:31930-43. 2010
    ..The production of miRNAs is believed to be dependent upon the DICER enzyme...
  76. doi A novel splice variant of the human dicer gene is expressed in neuroblastoma cells
    Nicoletta Potenza
    Department of Life Sciences, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy
    FEBS Lett 584:3452-7. 2010
    b>Dicer is a ribonuclease playing a key role in the biogenesis of microRNAs and small interfering RNAs. Here we report the identification of a novel splice variant of human dicer gene, the first one bearing a modified coding sequence...
  77. doi Association of a common AGO1 variant with lung cancer risk: a two-stage case-control study
    Jong Sik Kim
    Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
    Mol Carcinog 49:913-21. 2010
    ..In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients and 100 healthy controls ..
  78. pmc TAp63 suppresses metastasis through coordinate regulation of Dicer and miRNAs
    Xiaohua Su
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Nature 467:986-90. 2010
    ..Here we show that TAp63, a p53 family member, suppresses tumorigenesis and metastasis, and coordinately regulates Dicer and miR-130b to suppress metastasis...
  79. pmc MicroRNA-107, an oncogene microRNA that regulates tumour invasion and metastasis by targeting DICER1 in gastric cancer
    Xiaohua Li
    State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi an, China
    J Cell Mol Med 15:1887-95. 2011
    ..Inhibition of miR-107 or restoration of DICER1 may represent a new potential therapeutic target for gastric cancer treatment...
  80. doi Germline DICER1 mutations and familial cystic nephroma
    Amin Bahubeshi
    Program in Cancer Genetics, Department of Oncology, McGill University, Montreal, Quebec, Canada
    J Med Genet 47:863-6. 2010
    ..CN is rarely familial or bilateral, but it occurs in about 10% of families where pleuropulmonary blastoma (PPB) is present. Recently, germline mutations in DICER1 were found in familial PPB...
  81. doi MicroRNA loss enhances learning and memory in mice
    Witold Konopka
    Molecular Biology of the Cell I, German Cancer Research Center, D 69120 Heidelberg, Germany
    J Neurosci 30:14835-42. 2010
    b>Dicer-dependent noncoding RNAs, including microRNAs (miRNAs), play an important role in a modulation of translation of mRNA transcripts necessary for differentiation in many cell types...
  82. doi DICER1 syndrome: clarifying the diagnosis, clinical features and management implications of a pleiotropic tumour predisposition syndrome
    Ingrid Slade
    Section of Cancer Genetics, Institute of Cancer Research and Royal Marsden Hospital, 15 Cotswold Road, Sutton SM2 5NG, UK
    J Med Genet 48:273-8. 2011
    ..Constitutional DICER1 mutations were recently reported to cause familial pleuropulmonary blastoma (PPB)...
  83. pmc DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration
    Hiroki Kaneko
    Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
    Nature 471:325-30. 2011
    ..These findings reveal a miRNA-independent cell survival function for DICER1 involving retrotransposon transcript degradation, show that Alu RNA can directly cause human pathology, and identify new targets for a major cause of blindness...
  84. doi Overexpression of Dicer predicts poor survival in colorectal cancer
    C Faber
    Institute of Pathology, University of Munich, Germany
    Eur J Cancer 47:1414-9. 2011
    The RNASE III endonuclease Dicer is one of the key enzymes of microRNA biogenesis...
  85. pmc Ovarian sex cord-stromal tumors, pleuropulmonary blastoma and DICER1 mutations: a report from the International Pleuropulmonary Blastoma Registry
    Kris Ann P Schultz
    Department of Hematology Oncology, Children s Hospitals and Clinics of Minnesota, 2530 Chicago Ave S, CSC 175, Minneapolis, MN 55404, USA
    Gynecol Oncol 122:246-50. 2011
    ..Observations of ovarian sex cord-stromal tumors (OSCST) in PPB kindreds led to further study. We sought to characterize ovarian tumors seen in probands and families with PPB and PPB-related conditions and define germline DICER1 status...
  86. pmc Dicer and miRNA in relation to clinicopathological variables in colorectal cancer patients
    Johannes Stratmann
    Division of Biomedicine, Systems Biology Research Centre, School of Life Sciences, Skövde University, SE 541 28, Skovde, Sweden
    BMC Cancer 11:345. 2011
    b>Dicer is aberrantly expressed in several types of cancers...
  87. pmc Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells
    Yoshinari Ando
    RIKEN Omics Science Center, Yokohama, Kanagawa, Japan
    PLoS ONE 6:e23385. 2011
    ..Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein...
  88. doi Extending the phenotypes associated with DICER1 mutations
    William D Foulkes
    Program in Cancer Genetics, Department of Oncology, McGill University, Montreal, Quebec, Canada
    Hum Mutat 32:1381-4. 2011
    ..DICER1 appears to be the first gene implicated in the etiology of cERMS, cPNET, and PS. Young adulthood sarcomas and perhaps congenital malformations such as TGA may also be associated...
  89. doi The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma
    Xiaofang Guo
    Cancer Research Institute, Central South University, 110 Xiang Ya Road, Changsha, 410078, Hunan, People s Republic of China
    J Cancer Res Clin Oncol 138:49-56. 2012
    ..In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.
  90. pmc DICER1 mutations in embryonal rhabdomyosarcomas from children with and without familial PPB-tumor predisposition syndrome
    Leslie Doros
    Department of Pathology, Children s National Medical Center, Washington, District of Columbia 20010, USA
    Pediatr Blood Cancer 59:558-60. 2012
    ..8%) sporadic ERMS had somatic mutations. Our findings confirm the pathogenetic relationship between ERMS and PPB suggesting that ERMS may result from abnormal miRNA regulation...
  91. doi Recurrent somatic DICER1 mutations in nonepithelial ovarian cancers
    Alireza Heravi-Moussavi
    British Columbia Cancer Research Centre, Vancouver, BC, Canada
    N Engl J Med 366:234-42. 2012
    ..Mutation carriers are at risk for nonepithelial ovarian tumors, notably sex cord-stromal tumors...
  92. doi Downregulated Dicer expression predicts poor prognosis in chronic lymphocytic leukemia
    Dan Xia Zhu
    Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China
    Cancer Sci 103:875-81. 2012
    ..Alterations in microRNAs (miRNAs) expression have been proposed to play a role in CLL pathogenesis. Dicer and Drosha are the main regulators of miRNA biogenesis, and deregulation of their expression has been indicated as ..
  93. doi miRNA-130a targets ATG2B and DICER1 to inhibit autophagy and trigger killing of chronic lymphocytic leukemia cells
    Valentina Kovaleva
    University of Heidelberg, Heidelberg, Germany
    Cancer Res 72:1763-72. 2012
    ..Together, our findings show that miR-130a modulates cell survival programs by regulating autophagic flux, and they define roles for miR-130a and Dicer1 in a regulatory feedback loop that mediates CLL cell survival...
  94. doi Genetic variants in microRNA biogenesis pathway genes are associated with semen quality in a Han-Chinese population
    Yufeng Qin
    State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China
    Reprod Biomed Online 24:454-61. 2012
    ....
  95. doi Immunohistochemical analysis of the endoribonucleases Drosha, Dicer and Ago2 in smooth muscle tumours of soft tissues
    Dionysios J Papachristou
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Histopathology 60:E28-36. 2012
    ..the present study were to determine the protein levels and cellular distribution of the endoribonucleases Drosha, Dicer and Ago2, major components of the microRNA-processing machinery, in benign and malignant soft tissue smooth muscle ..
  96. doi Clinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer
    Taisuke Inoue
    Department of Surgery, Teikyo University School of Medicine, Tokyo 173 0003, Japan
    Oncol Rep 27:1759-64. 2012
    ..Our results indicate that miR-107 may be useful as an effective biomarker for prediction of a poor prognosis in gastric cancer patients...
  97. pmc The molecular architecture of human Dicer
    Pick Wei Lau
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, California, USA
    Nat Struct Mol Biol 19:436-40. 2012
    b>Dicer is a multidomain enzyme that generates small RNAs for gene silencing in eukaryotes...
  98. pmc In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs
    Allan M Gurtan
    David H Koch Institute for Integrative Cancer Research, Cambridge, Massachusetts 02139, USA
    RNA 18:1116-22. 2012
    b>Dicer is an RNase III family endoribonuclease and haploinsufficient tumor suppressor that processes mature miRNAs from the 5' (5p) or 3' (3p) arm of hairpin precursors...
  99. pmc Functional importance of Dicer protein in the adaptive cellular response to hypoxia
    J J David Ho
    Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5B 1W8, Canada
    J Biol Chem 287:29003-20. 2012
    ..Here, we show in vitro and in vivo that chronic hypoxia impairs Dicer (DICER1) expression and activity, resulting in global consequences on microRNA biogenesis...
  100. pmc Dicer-dependent biogenesis of small RNAs derived from 7SL RNA
    Yong feng Ren
    Institute of Genomic Medicine, Wenzhou Medical College, Wenzhou, People s Republic of China
    PLoS ONE 7:e40705. 2012
    It has been reported that decreased Dicer expression leads to Alu RNAs accumulation in human retinal pigmented epithelium cells, and Dicer may process the endogenous SINE/B1 RNAs (the rodent equivalent of the primate Alu RNAs) into small ..
  101. doi Loss of Dicer expression is associated with breast cancer progression and recurrence
    Sarkawt M Khoshnaw
    Department of Histopathology, School of Molecular Medical Sciences, University of Nottingham and Nottingham University Hospitals NHS Trust, Nottingham NG7 2UH, UK
    Breast Cancer Res Treat 135:403-13. 2012
    b>Dicer is a protein that plays a pivotal role in the final steps of the microRNA (miRNA) processing pathway, to produce mature miRNAs from their precursor molecules...

Research Grants71

  1. Ribonuclease III mechanism of double-strand RNA cleavage
    ALLEN NICHOLSON; Fiscal Year: 2003
    ..An important recent finding is the central involvement of the RNase III orthologue "Dicer" in RNA interference (RNAi)...
  2. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
    ..Eukaryotic RNase III orthologs include Dicer and Drosha, which carry out the maturation of microRNAs and other gene-regulatory RNAs...
  3. Eric C Lai; Fiscal Year: 2016
    ..Their biogenesis typically involves cleavage by the Drosha and Dicer RNase III enzymes, and the resultant mature miRNAs mediate extensive networks of post-transcriptional regulation...
  4. Scott A Showalter; Fiscal Year: 2015
    ..There are at least ten dsRBDs involved in miRNA production: two in DGCR8, one in Drosha, one in Dicer, three in TRBP, and three in PACT...
  5. miRNAs and epidermal langerhans cell development and function
    Qing Sheng Mi; Fiscal Year: 2011
    ..The ribonuclease III enzyme Dicer is required for the processing of mature and functional miRNAs...
  6. Wei Yan; Fiscal Year: 2014
    ..that when we inhibited the microRNA (miRNA) production by inactivating either of the two key RNase III enzymes, Dicer or Drosha, which are known to be essential for miRNA biosynthesis, Leydig cells could not function normally...
  7. Phillip D Zamore; Fiscal Year: 2014
    ..of their biogenesis began in 2001 with the discovery that the multi-domain ribonuclease III (RNase III) enzyme Dicer converts miRNA precursors (pre-miRNAs) into mature miRNAs...
  8. Daniel Cifuentes; Fiscal Year: 2014
    ..Typically, two RNase III family members, Drosha and Dicer, process the stem-loop structure of miRNA precursors sequentially...
  9. Phillip A Sharp; Fiscal Year: 2016
    ..For example, deletion of Dicer, a double strand endonuclease required for synthesis of most miRNAs, in many cancer cells yields viable cells that ..
  10. Antonio J Giraldez; Fiscal Year: 2015
    ..animals, miRNAs are derived from characteristic hairpins processed by two sequential RNAse III enzymes, Drosha and Dicer. Our recent work has identified a novel microRNA processing pathway independent of Dicer function that depends on ..
  11. Quantitative stepwise analysis of RNA interference
    Sua Myong; Fiscal Year: 2012
    ..The main components of the RNA silencing proteins include Dicer, TRBP and Argonaute (AGO), which make up the RNA induced silencing complex (RISC)...
  12. Molecular Mechanism of Small RNA-induced RNA Silencing
    Qinghua Liu; Fiscal Year: 2010
    ..We have previously identified two Dicer complexes, DCR-1/R3D1 and DCR-2/R2D2, as the respective miRNA- and siRNA-generating enzymes in Drosophila...
  13. Structure and Function of RNAi-related Nucleases
    Jennifer A Doudna; Fiscal Year: 2013
    ..Two specialized cytoplasmic ribonucleases function at either end of this pathway: the Dicer endonuclease catalyzes length-specific double-strand RNA cleavage to produce si- and miRNAs, whereas the Xrn1 ..
  14. HIV-1 TAR derived miRNA: Implications for Latency and Pathogenesis
    Fatah Kashanchi; Fiscal Year: 2010
    ..These cellular pre-miRNA are acted upon by the enzyme Dicer to yield miRNA that is capable of regulating gene expression...
  15. Yi Liu; Fiscal Year: 2015
    ..of sRNAs in this organism, including the DNA damage-induced qiRNA, first fungal miRNA-like sRNAs (milRNAs) and Dicer- independent small interfering RNAs (disiRNAs)...
  16. Phillip D Zamore; Fiscal Year: 2016
    ..Aim 1 will explore why Drosophila Dicer-1 and Dicer-2 use ATP. Dicer enzymes make siRNAs and miRNAs...
  17. Plasmon Rulers
    JAN T LIPHARDT; Fiscal Year: 2012
    ..we will use plasmon rulers to investigate the structural dynamics, substrate requirements, and mechanochemistry of Dicer, a central component of the RNA-induced silencing complex (RISC)...
  18. Richard W Carthew; Fiscal Year: 2014
    ..in the cytoplasm, thereby leading to the inactivation of the miRNA-protein complexes, and 4) understand how the Dicer ribonuclease cleaves RNA from infecting viruses to generate antiviral siRNAs...
  19. Characterization of TRBP-containing complexes
    Ramin Shiekhattar; Fiscal Year: 2009
    ..Our biochemical characterization of microRNA pathway revealed the association of the RNase III enzyme Dicer with the oncogenic protein TRBP...
  20. Generation of a potent kinase siRNA library using Dicer
    Zairen Sun; Fiscal Year: 2005
    ..This can be accomplished by synthesizing long double-stranded RNAs followed by cleavage with the Dicer enzyme. This would improve the efficacy to 100%. As a provider of genomics tools, OriGene Technologies, Inc...
  21. Elucidating Nuclear Argonaute Function
    Timothy J Kelly; Fiscal Year: 2013
    ..and (4) interrogate the chromatin modification and RNA expression differences between wildtype, Argonaute null and Dicer null ES cells...
  22. Role of microRNA in axon development
    Melissa L Hancock; Fiscal Year: 2012
    ..In preliminary studies, disruption of the miRNA processing enzyme, Dicer, resulted in axon defects including reduced growth rate, loss of guidance cue responsiveness, and disorganization ..
  23. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ..we will define the role of microRNAs in mediating HDI inhibition of autoimmunity using lupus-prone mice lacking Dicer or Drosha in AID+ B cells or lacking microRNA targeting sites in Aicda and Prdm1 mRNAs, and identify genome-wide ..
  24. Structure and Function of microRNA Gene Regulation Complexes
    Ross Wilson; Fiscal Year: 2013
    ..The focus of the research herein is to investigate the RLC proteins Dicer and TRBP, both of which occur at reduced or undetectable levels in cancer cells according to recent findings...
  25. Specification of alternate siRNA biogenesis pathways in Arabidopsis
    TODD LUCAS BLEVINS; Fiscal Year: 2011
    ..humans: RNA-dependent RNA polymerases (RDRs), which convert single-stranded RNA into double-stranded RNA (dsRNA), Dicer endonucleases that cleave dsRNA into short-interfering RNA (siRNA) duplexes, and Argonaute-containing complexes ..
  26. Micro RNA Expression and Cancer
    Thomas Schmittgen; Fiscal Year: 2005
    ..22 nt in length and is cleaved from partially duplexed precursors (termed pre-miRNA) by the ribonuclease III Dicer. Although over 100 human miRNAs have been discovered thus far, very little is known about their targets, levels of ..
  27. MicroRNA Modulation of Immune Function in Multiple Sclerosis
    Caroline C Whitacre; Fiscal Year: 2012
    ..Mice deficient in small RNA processing machinery, Dicer, produce more cytokines when their immune cells are activated...
  28. Role of micro RNAs in renin cell differentiation
    ROBERTO ARIEL GOMEZ; Fiscal Year: 2013
    ..Conditional deletion of Dicer in renin cells resulted in the disappearance of JG cells in the kidney suggesting that microRNAs may regulate ..
  29. microRNA Control of Wound Healing
    Chandan K Sen; Fiscal Year: 2013
    ..Hypothesis 1.1: Keratin-14 (K14) directed conditional ablation of Dicer in mice (K14CreERtam Dicer-/-) results in depletion of mature miRs in the skin epidermis and compromises re-..
  30. microRNA Uncoupling of Protein and Transcript Expression in Liver Regeneration
    CLIFFORD JOHN STEER; Fiscal Year: 2010
    ..This will be done by specific knockdown of critical components of Dicer in mina processing and Ago2 in the RNA Induced Silencing Complex (RISC)...
  31. microRNA regulation of spermatogonial stem cell self-renewal and differentiation
    RALPH LAWRENCE BRINSTER; Fiscal Year: 2013
    ..Abrogation of the miR processing protein, Dicer, in germ cells greatly reduces spermatogenesis, suggesting miRs are essential for the proliferation and ..
  32. Tatsuya Kobayashi; Fiscal Year: 2016
    ..miRNAs are first processed by the microprocessor containing Drosha and DGCR8 as critical components, and then by Dicer. Mature miRNAs are then loaded onto the RNA-induced silencing complex (RISC), that contains Argonaute (Ago) as the ..
  33. Karl H Obrietan; Fiscal Year: 2014
    ..Within the developing mammalian central nervous system, results from dicer null mice support a role for microRNAs in neuronal morphogenesis and neuronal survival...
  34. Lane K Christenson; Fiscal Year: 2014
    ..The long-term benefits of this research will enhance our understanding of the ovary, ultimately impacting the development of treatment strategies for infertility and new contraceptive methods for women. ..
  35. Role of Dicer in Gonadotrope and Reproductive Function
    T Rajendra Kumar; Fiscal Year: 2013
    ..Recently, micro RNAs (miRNAs) produced by Dicer, an RNA endonuclease III, have emerged as key regulators of gene expression, mRNA stability and protein synthesis...
  36. MicroRNA pathway in mechanisms of nanoparticles neurotoxicity
    Alexander K Murashov; Fiscal Year: 2010
    ..3) Investigate if miRNAs depletion will make neuron regeneration more vulnerable to CNTs exposures by deleting Dicer in vitro and in vivo...
  37. David P Bartel; Fiscal Year: 2016
    ..cerevisiae) and Candida albicans (a common human pathogen). These species use noncanonical Dicer proteins to process long dsRNA into small interfering RNAs (siRNAs), which are loaded into the Argonaute protein ..
  38. Amy DeMicco; Fiscal Year: 2015
    ..IR, through a mechanism that requires the tumor suppressors ATM and p53, and the microRNA (miR) processing enzyme Dicer. Preliminary data suggest that D3 expression is repressed primarily through increased mRNA turnover...
  39. Soonmoon Yoo; Fiscal Year: 2014
    ..Interestingly, several recent studies show the presence of Dicer and components of the miRNA-induced silencing complex (miRISC) that are required for processing precursor miRNAs (..
  40. Ales Cvekl; Fiscal Year: 2016
    ..proposal will (1) Determine the molecular functions of th FGF-responsive c-Maf promoter and Cryaa distal enhancer DCR1 followed by genome- wide identification of global FGF-regulated networks in the lens, (2) Establish ..
  41. Zheng Dong; Fiscal Year: 2016
    ..Using a conditional (renal proximal tubule specific) Dicer- knockout mouse model, we have recently demonstrated the first evidence for the involvement of microRNAs in ..
  42. MicroRNAs in Kidney Development
    Oliver Wessely; Fiscal Year: 2009
    ..for miRNAs in kidney development will be tested directly by eliminating the key component of miRNA biogenesis, Dicer, from Xenopus embryos...
  43. Elsa R Flores; Fiscal Year: 2015
    ..tumors, and TAp63 potently suppresses tumor metastasis by direct coordinate transcriptional regulation of Dicer and several miRNAs (Su et al., Nature 2010)...
  44. Circadian regulation of microRNA biogenesis and function
    Peter W Vanderklish; Fiscal Year: 2012
    ..2) "Antagomirs" and reporter constructs will be used to determine whether regulation of Drosha, Dicer and Ago2 by RBM3 involves direct effects on their translation, or feedback mechanism involving miRNAs that are ..
  45. Piotr Sliz; Fiscal Year: 2016
    ..During this post- transcriptional stage, Lin28 can block both of the cleavage steps (by Drosha and Dicer), and promote degradation by recruiting a terminal uridylyl transferase, specifically for the let-7 microRNAs...
  46. KATHRYN ANN WIKENHEISER-BROKAMP; Fiscal Year: 2014
    ....
  47. Maria Mudryj; Fiscal Year: 2014
    ..Additionally we found that E2F3b regulates the expression of the microRNA processing nuclease Dicer. Previous studies reported that Dicer expression is elevated in advanced and metastatic prostate tumors...
  48. Carl D Novina; Fiscal Year: 2015
    ..melanomas frequently up-regulate eIF4E, down-regulate miRNAs, down-regulate miRNA- associated proteins including Dicer and Argonautes (Agos), and therefore up-regulate miRNA-targeted oncogenes...
  49. Richard I Gregory; Fiscal Year: 2016
    ..miRNAs are produced from long primary transcripts through sequential cleavages by the Microprocessor and Dicer complexes...
  50. Role of microRNAs in initiation and progression of breast cancer
    LORENZO SEMPERE; Fiscal Year: 2010
    ..We will target chromosomal deletion of miRNA- processing enzyme Dicer in mammary gland epithelia using the Cre/LoxP system...
  51. Experimental identification of microRNA targets in mosquitoes during Plasmodium i
    Jinsong Zhu; Fiscal Year: 2012
    ..RNAi-mediated silencing of either Dicer-1 or Argonaute-1, key components of the microRNA pathway, has been shown to enhance parasite survival...
  52. A Cell Intrinsic, MicroRNA Driven Timer Restricts Dopaminergic Progenitors
    ANGELA MARIE ANDEREGG; Fiscal Year: 2012
    ..Specific Aim 1 involves removing Dicer, the enzyme necessary for processing all known microRNAs, with a Shh::Cre recombinase driver, which is active in ..
  53. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert H Blelloch; Fiscal Year: 2010
    ..This aim is based on the finding that the DGCR8 null ES cell phenotype differs from that of the Dicer null phenotype...
  54. Dana Ashley Hill; Fiscal Year: 2016
    ....
  55. microRNA regulation of endothelial functions
    William C Sessa; Fiscal Year: 2013
    ..Our previous work has shown that reduction of overall miRNA levels via Dicer silencing in human endothelial cells (EC) strongly regulates angiogenic gene expression and impairs aspects of in ..
  56. Mechanisms of Apoptosis Inhibition in Maturing Neurons: Focus on miR-29 and Dicer
    Adam J Kole; Fiscal Year: 2012
    ..abstract_text> ..
  57. The role of microRNAs in the development and differentiation of mouse photorecept
    NATECIA WILLIAMS; Fiscal Year: 2012
    ..Phenotypes resulting from the retinal deletion of Dicer, a critical enzyme required for small RNA processing, have established microRNAs (miRNAs), as key factors in ..
  58. MicroRNAs in Synaptic Plasticity and Cognitive Function
    Raymond J Kelleher; Fiscal Year: 2011
    ..To address these questions, we are employing a conditional genetic approach to inactivate Dicer and abolish miRNA expression in neurons of the postnatal forebrain...
  59. The link between mRNA 3'end processing and siRNA biogenesis in Drosophila
    Mindy Steiniger; Fiscal Year: 2013
    ..component of the core cleavage complex described in the previous paragraph, interacts with another protein, Dicer-2, to cut siRNAs from larger precursor RNAs...
  60. Phillip A Sharp; Fiscal Year: 2016
    ..roles of miRNAs in gene regulation including their mechanisms of action, the consequence of loss of all miRNAs in Dicer null cell lines, and the relationship between miRNAs and differentiation of mesenchymal tumors and stem cells...
  61. Conditional Dicer1 manipulation to study miRNA involvement in opioid addiction
    Norman H Lee; Fiscal Year: 2013
    ..The multi- tiered, multi-disciplinary approach (behavior, molecular, bioinformatics) is designed to probe an unchartered layer of the genetic architecture (miRNA) to discover novel molecular targets for therapeutic intervention. ..
  62. Danesh Moazed; Fiscal Year: 2016
    ..RNA polymerase complex (RDRC) to promote the synthesis of double stranded RNA, which is cleaved into siRNAs by the Dicer ribonuclease...
  63. MicroRNA Regulation of Stem Cell Self-renewal
    Shangqin Guo; Fiscal Year: 2013
    ..Disruption of the microRNA biogenesis key enzyme, Dicer, completely ablated stem cell activity...
  64. David M Ornitz; Fiscal Year: 2015
    ....
  65. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
    ..of this pre-miRNA that mediate the subsequent, equally specific cytoplasmic excision of the mature miRNA by dicer. In specific aim 2, we will extend our initial data showing that Exportin 5 (Exp5) is required for nuclear export ..
  66. microRNA function in human embryonic stem cells division
    Junlin Qi; Fiscal Year: 2009
    ..Towards reaching this goal, our preliminary data showed that hESCs lacking mature miRNAs due to knockdown (KD) of Dicer or Drosha have reduced cell cycle kinetics and delayed at both Gap-phases G1 and G2...
  67. MicroRNAs and Corneal Epithelial Stem Cells
    ROBERT LAVKER; Fiscal Year: 2007
    ..function of miRNAs during corneal epithelial development using tissue specific Cre expression to disrupt the Dicer-1 gene, which is essential for miRNA biogenesis...
  68. Role of microRNAs in genetic mouse models of pancreatic cancer
    Murray Korc; Fiscal Year: 2010
    ..In addition, global decreases in miRNA activity by loss of Dicer or other enzymes required for miRNA processing result in enhanced tumorigenic potential, reflecting on the overall ..
  69. Role of Micro-RNAs in Neuronal Circuit Formation and Function
    Erik Ullian; Fiscal Year: 2009
    ..Indeed, removing miRNAs early in development by knocking out the key RNAseIII enzyme required for their synthesis, Dicer, is lethal at the very early stages of gastrulation...
  70. Silencing Suppression Mechanism of a Herpes Simplex Virus Virion Component
    NIL CELEBI CHERUKURI; Fiscal Year: 2009
    ..components of the RNA-induced silencing complex (RISC), including TRBP (a PACT homolog), Argonaute 2 (Ago2), and Dicer. Protein interactions within cells will be determined by co-precipitation of transiently expressed, epitope-tagged ..
  71. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
    ..The effect of Dicer depletion on mitotic stability of HACs and endogenous chromosomes will be investigated...