DROSHA

Summary

Gene Symbol: DROSHA
Description: drosha ribonuclease III
Alias: ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN, ribonuclease 3, RNase III, drosha, double-stranded RNA-specific endoribonuclease, nuclear RNase III Drosha, p241, ribonuclease type III, nuclear
Species: human

Top Publications

  1. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
  2. pmc Histone deacetylase 1 enhances microRNA processing via deacetylation of DGCR8
    Taeko Wada
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical School, Shimotsuke, Tochigi 329 0498, Japan
    EMBO Rep 13:142-9. 2012
  3. ncbi A novel type of RNase III family proteins in eukaryotes
    V Filippov
    Department of Entomology, Graduate Programs in Biochemistry and Molecular Biology, and Genetics, University of California, Riverside, CA, USA
    Gene 245:213-21. 2000
  4. ncbi Human RNase III is a 160-kDa protein involved in preribosomal RNA processing
    H Wu
    Department of Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USA
    J Biol Chem 275:36957-65. 2000
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
  6. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
  7. pmc Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 24:138-48. 2005
  8. pmc The Drosha-DGCR8 complex in primary microRNA processing
    Jinju Han
    School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151 742, Korea
    Genes Dev 18:3016-27. 2004
  9. ncbi The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis
    Markus Landthaler
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Curr Biol 14:2162-7. 2004
  10. ncbi RNASEN regulates cell proliferation and affects survival in esophageal cancer patients
    Nobuyoshi Sugito
    Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan
    Clin Cancer Res 12:7322-8. 2006

Research Grants

  1. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
  2. Wei Yan; Fiscal Year: 2014
  3. Daniel Cifuentes; Fiscal Year: 2014
  4. Scott A Showalter; Fiscal Year: 2015
  5. Eric C Lai; Fiscal Year: 2016
  6. Antonio J Giraldez; Fiscal Year: 2015
  7. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
  8. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
  9. microRNA analysis of neurons generated from patient-specific iPSCs
    Herbert M Lachman; Fiscal Year: 2013
  10. Role of miRNAs in Vascular Physiology
    Akiko Hata; Fiscal Year: 2012

Detail Information

Publications298 found, 100 shown here

  1. pmc MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production
    Antony K Chen
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 111:E2676-83. 2014
    ....
  2. pmc Histone deacetylase 1 enhances microRNA processing via deacetylation of DGCR8
    Taeko Wada
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical School, Shimotsuke, Tochigi 329 0498, Japan
    EMBO Rep 13:142-9. 2012
    Relatively little is known about the regulatory mechanisms of the Drosha/DGCR8 complex, which processes miRNAs at the initial step of biogenesis...
  3. ncbi A novel type of RNase III family proteins in eukaryotes
    V Filippov
    Department of Entomology, Graduate Programs in Biochemistry and Molecular Biology, and Genetics, University of California, Riverside, CA, USA
    Gene 245:213-21. 2000
    The RNase III family of double-stranded RNA-specific endonucleases is characterized by the presence of a highly conserved 9 amino acid stretch in their catalytic center known as the RNase III signature motif...
  4. ncbi Human RNase III is a 160-kDa protein involved in preribosomal RNA processing
    H Wu
    Department of Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USA
    J Biol Chem 275:36957-65. 2000
    A human RNase III gene encodes a protein of 160 kDa with multiple domains, a proline-rich, a serine- and arginine-rich, and an RNase III domain...
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Dicer, a member of the RNase III superfamily of bidentate nucleases, mediates the latter step, whereas the processing enzyme for the former step ..
  6. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
    ..miRNAs are processed by the RNA-mediated interference machinery. Drosha is an RNase III enzyme that was recently implicated in miRNA processing...
  7. pmc Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 24:138-48. 2005
    ..human microRNA maturation is the processing of the primary microRNA transcript by the nuclear RNaseIII enzyme Drosha to generate the approximately 60-nucleotide precursor microRNA hairpin...
  8. pmc The Drosha-DGCR8 complex in primary microRNA processing
    Jinju Han
    School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151 742, Korea
    Genes Dev 18:3016-27. 2004
    RNase III proteins play key roles in microRNA (miRNA) biogenesis. The nuclear RNase III Drosha cleaves primary miRNAs (pri-miRNAs) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic RNase III Dicer to ..
  9. ncbi The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis
    Markus Landthaler
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Curr Biol 14:2162-7. 2004
    ..Drosha RNase III catalyzes the first excision event, the release in the nucleus of a hairpin RNA (pre-miRNA), which is followed ..
  10. ncbi RNASEN regulates cell proliferation and affects survival in esophageal cancer patients
    Nobuyoshi Sugito
    Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan
    Clin Cancer Res 12:7322-8. 2006
    ..It is possible that deregulation of miRNA may contribute to the oncogenesis of some cancers. We studied the expression level of the miRNA processing enzyme (DICER1, DGCR8, and RNASEN) in esophageal squamous cell carcinoma (ESCC).
  11. pmc Processing of intronic microRNAs
    Young Kook Kim
    Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea
    EMBO J 26:775-83. 2007
    ..The primary transcripts are cleaved by Drosha to release approximately 70 nt pre-miRNAs that are subsequently processed by Dicer to generate mature ..
  12. pmc Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing
    Tatsuya Nakamura
    Department of Molecular Virology, Immunology, and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210
    Proc Natl Acad Sci U S A 104:10980-5. 2007
    ..and continuing into excision of a stem-loop hairpin structure within pri-miRNA by the nuclear RNaseIII enzyme Drosha, transportation to the cytoplasm, and further processing by a second RNaseIII enzyme Dicer, into a 22-base mature ..
  13. ncbi Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins
    Aiko Shiohama
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan GSP Center, The Leading Institute of Keio University, Ibaraki, Japan
    Exp Cell Res 313:4196-207. 2007
    ..We detected at least four kinds of protein complex, such as DROSHA/DGCR8, DGCR8/Nucleolin, DGCR8/ILF3 and ILF3/XPO5, by co-immunoprecipitation...
  14. pmc SMAD proteins control DROSHA-mediated microRNA maturation
    Brandi N Davis
    Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nature 454:56-61. 2008
    ..promoting the processing of primary transcripts of miR-21 (pri-miR-21) into precursor miR-21 (pre-miR-21) by the DROSHA (also known as RNASEN) complex...
  15. pmc Integrative genomics analysis of chromosome 5p gain in cervical cancer reveals target over-expressed genes, including Drosha
    Luigi Scotto
    Department of Pathology, Columbia University Medical Center, New York, New York, USA
    Mol Cancer 7:58. 2008
    ..These changes may possess oncogenic properties by deregulating tumor-related genes. Gain of short arm of chromosome 5 (5p) is the most frequent karyotypic change in CC...
  16. pmc The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis
    Bin Yu
    Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 105:10073-8. 2008
    ..In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis...
  17. pmc Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma
    Yohei Horikawa
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 14:7956-62. 2008
    ..Compelling evidence has shown that miRNAs are involved in cancer initiation and progression. We hypothesized that genetic variations of the miRNA machinery genes could be associated with the risk of renal cell carcinoma...
  18. pmc Dicer, Drosha, and outcomes in patients with ovarian cancer
    William M Merritt
    University of Texas M D Anderson Cancer Center, Houston, TX 77230, USA
    N Engl J Med 359:2641-50. 2008
    We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.
  19. pmc Posttranscriptional crossregulation between Drosha and DGCR8
    Jinju Han
    School of Biological Sciences and National Creative Research Center, Seoul National University, Seoul 151 742, Korea
    Cell 136:75-84. 2009
    The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate...
  20. pmc Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8
    Archana Shenoy
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, and Department of Urology, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e6971. 2009
    The Microprocessor, containing the RNA binding protein Dgcr8 and RNase III enzyme Drosha, is responsible for processing primary microRNAs to precursor microRNAs...
  21. pmc Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha
    Brandi N Davis
    Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111, USA
    Mol Cell 39:373-84. 2010
    ..protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplored...
  22. pmc Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma
    Jie Lin
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Carcinogenesis 31:1805-12. 2010
    ..Haplotypes of DICER and DROSHA were also associated with altered patient survival and recurrence...
  23. pmc microRNA signature and expression of Dicer and Drosha can predict prognosis and delineate risk groups in neuroblastoma
    Ruey Jen Lin
    Graduate Institute of Life Science, National Defense Medical Center, and Genomics Research Center, Academia Sinica, Taipei, Taiwan
    Cancer Res 70:7841-50. 2010
    ..To investigate the microRNA (miRNA) profile and role of Dicer and Drosha in neuroblastoma, we assessed the expression of 162 human miRNAs, Dicer and Drosha in 66 neuroblastoma tumors by ..
  24. pmc Expanding the role of Drosha to the regulation of viral gene expression
    Yao Tang Lin
    Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712 0162, USA
    Proc Natl Acad Sci U S A 108:11229-34. 2011
    ..Here, we show that Drosha, an endoribonuclease best known for its role in the biogenesis of microRNAs (miRNAs), can also function to ..
  25. doi Dysregulated expression of dicer and drosha in breast cancer
    Min Yan
    Department of Oncology, First Affiliated Hospital of Zhengzhou University, No 1, East Jianshe Rd, Zhengzhou, 450003, People s Republic of China
    Pathol Oncol Res 18:343-8. 2012
    ..Altered expression of Dicer and Drosha, two key enzymes in the miRNA maturation, is believed to be one of the most important mechanisms...
  26. doi Genetic variants in microRNA biogenesis pathway genes are associated with semen quality in a Han-Chinese population
    Yufeng Qin
    State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China
    Reprod Biomed Online 24:454-61. 2012
    ..Seven single-nucleotide polymorphisms (SNP) of DICER1 (rs13078, rs1057035 and rs12323635) and DROSHA (rs10719, rs2291109, rs17409893 and rs642321) were determined by TaqMan probes and SNPstream in 667 eligible ..
  27. doi Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder
    Yonghua Han
    Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, China Institute of Urology, Shenzhen PKU HKUST Medical Center, Shenzhen, China
    J Surg Oncol 107:201-5. 2013
    MicroRNAs (miRNAs) are aberrantly expressed in cancers. Dicer, Drosha, and Exportin 5 are essential for miRNA processing...
  28. pmc DGCR8 HITS-CLIP reveals novel functions for the Microprocessor
    Sara Macias
    Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, UK
    Nat Struct Mol Biol 19:760-6. 2012
    The Drosha-DGCR8 complex (Microprocessor) is required for microRNA (miRNA) biogenesis. DGCR8 recognizes the RNA substrate, whereas Drosha functions as the endonuclease...
  29. doi The microRNA maturation regulator Drosha is an independent predictor of outcome in breast cancer patients
    Sarkawt M Khoshnaw
    Department of Histopathology, School of Molecular Medical Sciences, University of Nottingham, Nottingham, UK
    Breast Cancer Res Treat 137:139-53. 2013
    b>Drosha is a protein that plays a key role in the biogenesis of microRNAs which are well known to be deranged in human breast cancer (BC)...
  30. pmc Drosha regulates gene expression independently of RNA cleavage function
    Natalia Gromak
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK Electronic address
    Cell Rep 5:1499-510. 2013
    Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus...
  31. pmc Recurrent somatic mutation in DROSHA induces microRNA profile changes in Wilms tumour
    Giovana T Torrezan
    1 Genomics and Molecular Biology Laboratory, International Research Center, A C Camargo Cancer Center, Sao Paulo, S P, 01508 010, Brazil 2
    Nat Commun 5:4039. 2014
    ..Here we identify DROSHA mutations in 12% of WT samples (26/222) using whole-exome sequencing and targeted sequencing of 10 microRNA (miRNA)..
  32. pmc Alternative initiation and splicing in dicer gene expression in human breast cells
    Charletha V Irvin-Wilson
    Division of Cancer Biology, Department of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208, USA
    Breast Cancer Res 7:R563-9. 2005
    ....
  33. doi Association of a common AGO1 variant with lung cancer risk: a two-stage case-control study
    Jong Sik Kim
    Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
    Mol Carcinog 49:913-21. 2010
    ..In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients ..
  34. pmc Major regulators of microRNAs biogenesis Dicer and Drosha are down-regulated in endometrial cancer
    Anna Torres
    Laboratory of Biostructure, Department of Human Anatomy, Medical University of Lublin, ul Jaczewskiego 4, 20 094, Lublin, Poland
    Tumour Biol 32:769-76. 2011
    Alterations in microRNAs expression have been proposed to play role in endometrial cancer pathogenesis. Dicer and Drosha are main regulators of microRNA biogenesis and deregulation of their expression has been indicated as a possible ..
  35. ncbi MicroRNA-206 is overexpressed in the diaphragm but not the hindlimb muscle of mdx mouse
    John J McCarthy
    Dept of Physiology, University of Kentucky Medical Center, 800 Rose St, Lexington, KY 40536 0298, USA
    Am J Physiol Cell Physiol 293:C451-7. 2007
    ..Transcript levels of Drosha and Dicer, major components of microRNA biogenesis pathway, were unchanged in mdx muscle, suggesting the pathway ..
  36. pmc Prediction of guide strand of microRNAs from its sequence and secondary structure
    Firoz Ahmed
    Bioinformatics Centre, Institute of Microbial Technology, Chandigarh, India
    BMC Bioinformatics 10:105. 2009
    ..miRNAs) are produced by the sequential processing of a long hairpin RNA transcript by Drosha and Dicer, an RNase III enzymes, and form transitory small RNA duplexes...
  37. pmc Hyaluronan-CD44 interaction with protein kinase C(epsilon) promotes oncogenic signaling by the stem cell marker Nanog and the Production of microRNA-21, leading to down-regulation of the tumor suppressor protein PDCD4, anti-apoptosis, and chemotherapy res
    Lilly Y W Bourguignon
    Endocrine Unit, Department of Medicine, University of California at San Francisco, and Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 284:26533-46. 2009
    ..Phosphorylated Nanog is then translocated from the cytosol to the nucleus and becomes associated with RNase III DROSHA and the RNA helicase p68...
  38. ncbi Structural basis for non-catalytic and catalytic activities of ribonuclease III
    Xinhua Ji
    Macromolecular Crystallography Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Acta Crystallogr D Biol Crystallogr 62:933-40. 2006
    Ribonuclease III (RNase III) represents a highly conserved family of double-stranded (ds) RNA-specific endoribonucleases, exemplified by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer...
  39. doi Mini-III, an unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in B. subtilis
    Yulia Redko
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Mol Microbiol 68:1096-106. 2008
    ..subtilis 23S rRNA is also performed by an enzyme that is absent from the Proteobacteria. Mini-III contains an RNase III-like catalytic domain, but curiously lacks the double-stranded RNA binding domain typical of RNase III itself, ..
  40. pmc MicroRNA processing pathway regulates olfactory neuron morphogenesis
    Daniela Berdnik
    Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, CA 94305, USA
    Curr Biol 18:1754-9. 2008
    ..The nuclear RNase III Drosha catalyzes the first processing step together with the dsRNA binding protein DGCR8/Pasha generating pre-..
  41. doi The evolutionary study of small RNA-directed gene silencing pathways by investigating RNase III enzymes
    Lihua Jin
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Yata, Mishima, Shizuoka, Japan
    Gene 435:1-8. 2009
    ..Here we investigated the evolution of RNase III nucleases, Dicer as representative, to further figure out the evolutionary relationship of these three gene ..
  42. doi MicroRNAs and cancer: past, present, and potential future
    Kristen M Nelson
    The Translational Genomics Research Institute, 10510 North 92nd Street, Suite 200, Scottsdale, AZ 85238, USA
    Mol Cancer Ther 7:3655-60. 2008
    ..After being processed by Drosha and Dicer RNase III endonucleases, mature miRNAs can inhibit the translation of mRNA by directing a RNA-induced silencing complex (..
  43. ncbi The role of microRNAs in cancer: no small matter
    Erik A C Wiemer
    Department of Medical Oncology, Josephine Nefkens Institute, Erasmus Medical Center, 3015 GE Rotterdam, The Netherlands
    Eur J Cancer 43:1529-44. 2007
    ..primary transcripts by two sequential processing steps mediated by a nuclear (Drosha) and a cytoplasmic (Dicer) RNAse III endonuclease...
  44. pmc The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila
    Katsutomo Okamura
    Memorial Sloan Kettering Cancer Center, Department of Developmental Biology, 1275 York Ave, Box 252, New York, NY 10021, USA
    Cell 130:89-100. 2007
    ..hairpin precursor transcripts into approximately 22 nucleotide regulatory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer...
  45. pmc Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization
    Xiaoli Tang
    Laboratory of Retrovirology, Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School of Brown University, Providence, RI 02903, USA
    Nucleic Acids Res 38:6610-9. 2010
    The RNaseIII enzyme Drosha plays a pivotal role in microRNA (miRNA) biogenesis by cleaving primary miRNA transcripts to generate precursor miRNA in the nucleus...
  46. pmc A high-throughput method to monitor the expression of microRNA precursors
    Thomas D Schmittgen
    Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA
    Nucleic Acids Res 32:e43. 2004
    ..The approximately 22 nt mature miRNA is processed from the pre-miRNA by the RNase III Dicer...
  47. ncbi Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
    ..Initial cleavage is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus...
  48. ncbi Ribonuclease revisited: structural insights into ribonuclease III family enzymes
    Ian J Macrae
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Curr Opin Struct Biol 17:138-45. 2007
    Ribonuclease III (RNase III) enzymes occur ubiquitously in biology and are responsible for processing RNA precursors into functional RNAs that participate in protein synthesis, RNA interference and a range of other cellular activities...
  49. doi Altered eIF6 and Dicer expression is associated with clinicopathological features in ovarian serous carcinoma patients
    Richard J Flavin
    Department of Histopathology, Trinity College Dublin, Dublin, Ireland
    Mod Pathol 21:676-84. 2008
    ..Dicer and Drosha (RNase III endonucleases) are essential components of the microRNA machinery...
  50. doi Treatment with small interfering RNA affects the microRNA pathway and causes unspecific defects in zebrafish embryos
    Xiao Feng Zhao
    Department of Molecular Biology, University of Bergen, Norway
    FEBS J 275:2177-84. 2008
    MicroRNAs (miRNAs) are generated from primary transcripts through sequential processing by two RNase III enzymes, Drosha and Dicer, in association with other proteins...
  51. doi New approaches to understanding double-stranded RNA processing by ribonuclease III purification and assays of homodimeric and heterodimeric forms of RNase III from bacterial extremophiles and mesophiles
    Wenzhao Meng
    Department of Chemistry, Temple University, Philadelphia, Pennsylvania, USA
    Methods Enzymol 447:119-29. 2008
    Ribonuclease III (RNase III) is a double-stranded (ds)-RNA-specific endonuclease that plays essential roles in the maturation and decay of coding and noncoding RNAs...
  52. pmc MD simulations of the dsRBP DGCR8 reveal correlated motions that may aid pri-miRNA binding
    Christopher Wostenberg
    Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA
    Biophys J 99:248-56. 2010
    ..dsRBDs) in tandem, is vital for nuclear maturation of primary miRNAs (pri-miRNAs) in connection with the RNase III enzyme Drosha...
  53. pmc Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis
    Jr Shiuan Yang
    Department of Developmental Biology, Sloan Kettering Institute, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:15163-8. 2010
    ..animal microRNAs (miRNAs) are generated by sequential cleavage of precursor substrates by the Drosha and Dicer RNase III enzymes. Several variant pathways exploit other RNA metabolic activities to generate functional miRNAs...
  54. pmc Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
    Klaus Forstemann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 3:e236. 2005
    ..Primary miRNA (pri-miRNA) transcripts are transformed to mature miRNA by the successive actions of two RNase III endonucleases...
  55. pmc Coupled RNA processing and transcription of intergenic primary microRNAs
    Monica Ballarino
    University of Rome La Sapienza, P le A Moro 5, 00185 Rome, Italy
    Mol Cell Biol 29:5632-8. 2009
    ..microRNA (miRNA) biogenesis occurs in the nucleus and is mediated by the Microprocessor complex containing the RNase III-like enzyme Drosha and its cofactor DGCR8...
  56. pmc Dicer-independent, Ago2-mediated microRNA biogenesis in vertebrates
    Jr Shiuan Yang
    Sloan Kettering Institute, Department of Developmental Biology, Cornell University, New York, NY, USA
    Cell Cycle 9:4455-60. 2010
    A canonical biogenesis pathway involving sequential cleavage by the Drosha and Dicer RNAse III enzymes governs the maturation of most animal microRNAs...
  57. pmc The estrogen receptor-alpha-induced microRNA signature regulates itself and its transcriptional response
    Leandro Castellano
    Department of Oncology, Cyclotron Building, Hammersmith Hospital Campus, Imperial College, Du Cane Road, London W12 0NN, United Kingdom
    Proc Natl Acad Sci U S A 106:15732-7. 2009
    ..Pri-mir-17-92 is immediately cleaved by DROSHA to pre-miR-18a, indicating that its regulation occurs during the formation of the mature molecule from the ..
  58. ncbi [Prognostic significance of metastatic lymph node ratio in colorectal cancer]
    Yang Zhao
    Department of Colorectal Surgery, Zhejiang Cancer Hospital, Hangzhou 310022, China
    Zhonghua Zhong Liu Za Zhi 31:764-8. 2009
    ..To investigate the prognostic significance of metastatic lymph node ratio in patients with colorectal cancer...
  59. ncbi Intracellular siRNA and precursor miRNA trafficking using bioresponsive copolypeptides
    Ulrik L Rahbek
    Department of Molecular Biology, University of Aarhus, DK 8000, Denmark
    J Gene Med 10:81-93. 2008
    ....
  60. ncbi Novel mutual prodrug of retinoic and butyric acids with enhanced anticancer activity
    A Nudelman
    Chemistry Department, Bar Ilan University, Ramat Gan, Israel
    J Med Chem 43:2962-6. 2000
    ..of ATRA compared to that of the acyloxylalkyl esters derived from butyric (RN1), propionic (RN2), isobutyric (RN3), and pivalic (RN4) acids was found to be: RN1 > RN2 > RN3 > ATRA approximately RN4...
  61. pmc Distinct microRNA alterations characterize high- and low-grade bladder cancer
    James W F Catto
    Academic Urology Unit, University of Sheffield, Sheffield, United Kingdom
    Cancer Res 69:8472-81. 2009
    ..This was associated with upregulation of Dicer, Drosha, and Exportin 5...
  62. pmc Prediction of novel precursor miRNAs using a context-sensitive hidden Markov model (CSHMM)
    Sumeet Agarwal
    Systems Biology Doctoral Training Centre and Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford OX1 3PU, UK
    BMC Bioinformatics 11:S29. 2010
    ..We also demonstrate how this model can be used in conjunction with filters as an ab initio method for miRNA identification...
  63. ncbi MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy
    John J McCarthy
    Department of Physiology, University of Kentucky Medical Center, 800 Rose St, Lexington, KY 40536 0298, USA
    J Appl Physiol (1985) 102:306-13. 2007
    ..overload was not explained by a change in the expression of components of the miRNA biogenesis pathway, since Drosha and Exportin-5 transcript levels were significantly increased by 50% in response to functional overload, whereas ..
  64. pmc Functional association of the Microprocessor complex with the spliceosome
    Naoyuki Kataoka
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Mol Cell Biol 29:3243-54. 2009
    ..introns were spliced more slowly than the adjacent introns, a model was previously proposed in which Drosha crops the pre-miRNA and the two cropped fragments from the pre-mRNA are subsequently trans spliced (Y. K...
  65. pmc microRNAs regulate human embryonic stem cell division
    Junlin Qi
    Department of Biochemistry and Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA
    Cell Cycle 8:3729-41. 2009
    ..in regulating processes relevant to embryonic stem cell biology, we used RNA interference to silence DICER and DROSHA, the two main miRNA processing enzymes...
  66. ncbi Apoptosis induction by antisense oligonucleotides against miR-17-5p and miR-20a in lung cancers overexpressing miR-17-92
    H Matsubara
    Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Oncogene 26:6099-105. 2007
    ..Finally, this study also revealed that the vast majority of C13orf25 transcripts are detected as Drosha-processed cleavage products on Northern blot analysis and that a novel polyadenylation site is present 3' to the ..
  67. pmc Extensive variation between inbred mouse strains due to endogenous L1 retrotransposition
    Keiko Akagi
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA
    Genome Res 18:869-80. 2008
    ..structures and expression are altered directly by polymorphic L1 retrotransposons, including Drosha (also called Rnasen), Parp8, Scn1a, Arhgap15, and others, including novel genes...
  68. pmc Biogenesis of short intronic repeat 27-nucleotide small RNA from endothelial nitric-oxide synthase gene
    Ming Xiang Zhang
    Adult Section of Cardiothoracic Surgery, Texas Heart Institute at St Luke s Episcopal Hospital, Division of Cardiothoracic Surgery, Michael E DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    J Biol Chem 283:14685-93. 2008
    ..Suppression of Drosha or Dicer diminished the biogenesis of the 27nt sir-RNA...
  69. pmc Sequence features associated with microRNA strand selection in humans and flies
    Hai yang Hu
    Partner Institute for Computational Biology, 320 Yue Yang Road, Shanghai, 200031, PR China
    BMC Genomics 10:413. 2009
    During microRNA (miRNA) maturation in humans and flies, Drosha and Dicer cut the precursor transcript, thereby producing a short RNA duplex...
  70. pmc Adenosine deamination in human transcripts generates novel microRNA binding sites
    Glen M Borchert
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Hum Mol Genet 18:4801-7. 2009
    ..A-to-I editing can affect Drosha processing or directly alter the microRNA (miRNA) sequences responsible for mRNA targeting...
  71. pmc EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis
    Eleanor M Maine
    Department of Biology, Syracuse University, Syracuse, New York 13244, USA
    Curr Biol 15:1972-8. 2005
    ..This H3K9me2 enrichment does not require Dicer or Drosha nuclease or any of several other proteins required for RNAi...
  72. ncbi MicroRNAs in disease and potential therapeutic applications
    Harris S Soifer
    1Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Mol Ther 15:2070-9. 2007
    ..are created from precursor transcripts by subsequent processing steps mediated by members of the RNAseIII family, Drosha and Dicer...
  73. ncbi MicroRNA biogenesis: drosha can't cut it without a partner
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 15:R61-4. 2005
    The ribonuclease Drosha requires a dedicated double-stranded RNA binding protein to convert long, nuclear primary microRNA transcripts into shorter pre-microRNA stem-loops, the cytoplasmic precursors from which mature microRNAs are ..
  74. pmc Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage
    Zhihua Du
    Departments of Pharmaceutical Chemistry and Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 105:2391-6. 2008
    Dicer, an RNase III enzyme, initiates RNA interference by processing precursor dsRNAs into mature microRNAs and small-interfering RNAs. It is also involved in loading and activation of the RNA-induced silencing complex...
  75. pmc Low-density Taqman miRNA array reveals miRNAs differentially expressed in prostatic stem cells and luminal cells
    Li Zhang
    Department of Molecular and Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Prostate 70:297-304. 2010
    ..miRNAs are a class of naturally occurring small RNAs that generally repress gene expression. They have been shown to actively control diverse biological processes including stem cell differentiation and lineage commitment...
  76. pmc MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila
    Alex S Flynt
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, Box 252, New York, NY 10065, USA
    Mol Cell 38:900-7. 2010
    microRNAs (miRNAs) are approximately 22 nucleotide regulatory RNAs derived from hairpins generated either by Drosha cleavage (canonical substrates) or by splicing and debranching of short introns (mirtrons)...
  77. doi Downregulated Dicer expression predicts poor prognosis in chronic lymphocytic leukemia
    Dan Xia Zhu
    Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China
    Cancer Sci 103:875-81. 2012
    ..Alterations in microRNAs (miRNAs) expression have been proposed to play a role in CLL pathogenesis. Dicer and Drosha are the main regulators of miRNA biogenesis, and deregulation of their expression has been indicated as a possible ..
  78. pmc Artificial mirtron-mediated gene knockdown: functional DMPK silencing in mammalian cells
    Yiqi Seow
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, United Kingdom
    RNA 18:1328-37. 2012
    ..are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs...
  79. pmc TGF-β and iron differently alter HBV replication in human hepatocytes through TGF-β/BMP signaling and cellular microRNA expression
    Sun O Park
    Department of Medicine, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA
    PLoS ONE 7:e39276. 2012
    ..e., SMADs, likely governed iron or TGF-β-induced microRNA expression. Iron may have mediated Drosha/DGCR8/heme-mediated processing of microRNAs...
  80. doi DICER1, DROSHA and miRNAs in patients with non-small cell lung cancer: implications for outcomes and histologic classification
    C Vanesa Díaz-García
    Oncology Department, Hospital Universitario 12 de Octubre, Madrid, 28041, Spain
    Carcinogenesis 34:1031-8. 2013
    ..In this study, we investigated whether the expression levels of DICER1 and DROSHA, components of the RNA-interference machinery, can predict survival, and whether the miRNA expression profiles can ..
  81. pmc Exosomes derived from HIV-1-infected cells contain trans-activation response element RNA
    Aarthi Narayanan
    National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia 20110, USA
    J Biol Chem 288:20014-33. 2013
    ..We detected the host miRNA machinery proteins Dicer and Drosha in exosomes from infected cells...
  82. pmc Phosphorylation of DGCR8 increases its intracellular stability and induces a progrowth miRNA profile
    Kristina M Herbert
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Cell Rep 5:1070-81. 2013
    During miRNA biogenesis, the microprocessor complex (MC), which is composed minimally of Drosha, an RNase III enzyme, and DGCR8, a double-stranded RNA-binding protein, cleaves the primary miRNA (pri-miRNA) in order to release the pre-..
  83. doi Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing
    Chase L Beisel
    Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, CA, USA
    Methods Mol Biol 1111:259-67. 2014
    ..Individual miRNAs also can be incorporated into the same transcript for tunable, multi-gene silencing. ..
  84. doi MeCP2: multifaceted roles in gene regulation and neural development
    Tian Lin Cheng
    Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
    Neurosci Bull 30:601-9. 2014
    ..MeCP2 represses nuclear microRNA processing by interacting directly with the Drosha/DGCR8 complex...
  85. doi EIF2C, Dicer, and Drosha are up-regulated along tumor progression and associated with poor prognosis in bladder carcinoma
    Zhe Zhang
    Department of Urology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang City, Liaoning Province, 110001, People s Republic of China
    Tumour Biol 36:5071-9. 2015
    EIF2C, Dicer, and Drosha are microRNA-regulating machinery components, which participate in microRNA intracellular process and transfer...
  86. pmc Stress induces p38 MAPK-mediated phosphorylation and inhibition of Drosha-dependent cell survival
    Qian Yang
    Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi an, Shaanxi 710038, China Department of Pharmacology, Emory University School of Medicine, Atlanta, GA 30322, USA Electronic address
    Mol Cell 57:721-34. 2015
    ..The key step in canonical miRNA biogenesis is the cleavage of the primary transcripts by the nuclear RNase III enzyme Drosha. Emerging evidence suggests that the miRNA biogenic cascade is tightly controlled...
  87. ncbi [Effect of estrogen or progesterone combined with paclitaxel on human ovarian cancer cell growth and Drosha expression]
    Yunjie Yang
    Department of Obstetrics and Gynecology, Huaian Maternity and Child Healh Care Hospital, Huai an 223002, China
    Zhonghua Zhong Liu Za Zhi 37:578-84. 2015
    To investigate the effect of estrogen (E2), progesterone(P4), and paclitaxel (taxol) on the growth of primary human ovarian cancer cells in vitro and the expression of Drosha.
  88. pmc Splicing-dependent expression of microRNAs of mirtron origin in human digestive and excretory system cancer cells
    Stasė Butkytė
    Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania
    Clin Epigenetics 8:33. 2016
    An abundant class of intronic microRNAs (miRNAs) undergoes atypical Drosha-independent biogenesis in which the spliceosome governs the excision of hairpin miRNA precursors, called mirtrons...
  89. pmc IKKα-mediated biogenesis of miR-196a through interaction with Drosha regulates the sensitivity of cancer cells to radiotherapy
    X Fang
    Xiangya Hospital, Central South University, Hunan 410008, China
    Cell Death Differ 23:1471-82. 2016
    ..Here we show that IKKα-mediated miR-196a biogenesis via interaction with Drosha regulates the sensitivity of nasopharyngeal carcinoma (NPC) cells to radiotherapy...
  90. pmc Argonaute-associated short introns are a novel class of gene regulators
    Thomas B Hansen
    Department of Molecular Biology and Genetics MBG, Interdisciplinary Nanoscience Center iNANO, Aarhus University, C F Moellers Allé 3, build 1130, Aarhus 8000, Denmark
    Nat Commun 7:11538. 2016
    ..Mature miRNAs are produced by two sequential endonucleolytic cleavages facilitated by Drosha in the nucleus and Dicer in the cytoplasm...
  91. doi Folate Receptor Alpha Upregulates Oct4, Sox2 and Klf4 and Downregulates miR-138 and miR-let-7 in Cranial Neural Crest Cells
    Vineet Mohanty
    Developmental Biology Program, Stanley Manne Children s Research Institute, Division of Pediatric Neurosurgery, Ann and Robert H Lurie Children s Hospital of Chicago, Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA
    Stem Cells . 2016
    ..Co-immunoprecipitation data suggests that FRα interacts with the Drosha-DGCR8 complex to affect pre-miRNA processing...
  92. doi Negative feedback circuitry between MIR143HG and RBM24 in Hirschsprung disease
    Chunxia Du
    State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China Department of Pediatric Surgery, Nanjing Children s Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China
    Biochim Biophys Acta 1862:2127-2136. 2016
    ..In addition, experiments using siRNA against DROSHA indicated that RBM24 could promote the biogenesis of miR-143...
  93. pmc Forward and reverse genetics through derivation of haploid mouse embryonic stem cells
    Ulrich Elling
    IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, 1030 Vienna, Austria
    Cell Stem Cell 9:563-74. 2011
    ..This system allowed us to generate a knockout cell line for the microRNA processing enzyme Drosha. In a forward genetic screen, we identified Gpr107 as a molecule essential for killing by ricin, a toxin being ..
  94. doi The development of Wilms tumor: from WT1 and microRNA to animal models
    Fang Tian
    Department of Pathophysiology, School of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, PR China
    Biochim Biophys Acta 1846:180-7. 2014
    ..Recent investigations also revealed that mutations of Drosha and Dicer, the RNases required for miRNA generation, and Dis3L2, the 3'-5' exonuclease that normally degrades ..
  95. doi Homologous cloning, purification and characterization of highly active cellobiohydrolase I (Cel7A) from Penicillium canescens
    Pavel V Volkov
    A N Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr 33, Moscow 119071, Russia
    Protein Expr Purif 103:1-7. 2014
    ..Cellobiohydrolase I of P. canescens (PcCel7A) was homologously cloned into a host strain RN3-11-7 (niaD-) and then expressed under the control of a strong xylA promoter...
  96. pmc Early postnatal ablation of the microRNA-processing enzyme, Drosha, causes chondrocyte death and impairs the structural integrity of the articular cartilage
    T Kobayashi
    Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA Electronic address
    Osteoarthritis Cartilage 23:1214-20. 2015
    ..the possibility of generating a novel mouse osteoarthritis (OA) model caused by intrinsic cellular dysfunction, we ablated Drosha, another essential enzyme for miRNA biogenesis, exclusively in articular chondrocytes of postnatal mice.
  97. pmc cis-Acting effects on RNA processing and Drosha cleavage prevent Epstein-Barr virus latency III BHRF1 expression
    Li Xing
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 85:8929-39. 2011
    ..Herein we report that Drosha cleavage of LTIII BHRF1 RNA and cis-acting splicing effects inhibit splicing and inhibit BHRF1 RNA and protein ..
  98. doi Microprocessor dynamics and interactions at endogenous imprinted C19MC microRNA genes
    Clément Bellemer
    Laboratoire de Biologie Moléculaire Eucaryote LBME, Université Paul Sabatier UPS, Universite de Toulouse, 31000 Toulouse, France
    J Cell Sci 125:2709-20. 2012
    Nuclear primary microRNA (pri-miRNA) processing catalyzed by the DGCR8-Drosha (Microprocessor) complex is highly regulated. Little is known, however, about how microRNA biogenesis is spatially organized within the mammalian nucleus...
  99. doi Correlation between hepatitis B virus protein and microRNA processor Drosha in cells expressing HBV
    Min Ren
    Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
    Antiviral Res 94:225-31. 2012
    b>Drosha regulates the biogenesis of microRNAs (miRNAs) and plays an essential role in the regulation of gene expression. Infection with hepatitis B virus (HBV) causes chronic hepatitis and liver cirrhosis...
  100. pmc Evidence for a cytoplasmic microprocessor of pri-miRNAs
    Jillian S Shapiro
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    RNA 18:1338-46. 2012
    ..in c-pri-miRNA cleavage while genetic studies revealed that maturation still relied on the canonical nuclear RNase III enzyme, Drosha...
  101. doi Small RNAs: emerging key players in DNA double-strand break repair
    Zhaoqing Ba
    Tsinghua Peking Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China
    Sci China Life Sci 56:933-6. 2013
    ..DSB-induced small RNAs (diRNAs) or Dicer/Drosha-dependent RNAs (DDRNAs) have been recently discovered in plants and vertebrates, adding an unsuspected RNA ..

Research Grants36

  1. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
    ..dsRNA cleavage is catalyzed by members of the ribonuclease III (RNase III) family of endoribonucleases, which are highly conserved in bacterial and eukaryotic cells...
  2. Wei Yan; Fiscal Year: 2014
    ..in mice that when we inhibited the microRNA (miRNA) production by inactivating either of the two key RNase III enzymes, Dicer or Drosha, which are known to be essential for miRNA biosynthesis, Leydig cells could not ..
  3. Daniel Cifuentes; Fiscal Year: 2014
    ..Typically, two RNase III family members, Drosha and Dicer, process the stem-loop structure of miRNA precursors sequentially...
  4. Scott A Showalter; Fiscal Year: 2015
    ..miRNA processing in the cell involves two ribonucleoprotein complexes composed of RNase III enzymes and double-stranded RNA binding domain (dsRBD) chaperones that work together to achieve two highly ..
  5. Eric C Lai; Fiscal Year: 2016
    ..Their biogenesis typically involves cleavage by the Drosha and Dicer RNase III enzymes, and the resultant mature miRNAs mediate extensive networks of post-transcriptional regulation...
  6. Antonio J Giraldez; Fiscal Year: 2015
    ..In animals, miRNAs are derived from characteristic hairpins processed by two sequential RNAse III enzymes, Drosha and Dicer...
  7. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
    ..intermediate from this initial transcript, a processing event very recently proposed to be mediated by human RNAse III/drosha...
  8. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ..define the role of microRNAs in mediating HDI inhibition of autoimmunity using lupus-prone mice lacking Dicer or Drosha in AID+ B cells or lacking microRNA targeting sites in Aicda and Prdm1 mRNAs, and identify genome-wide HDI-..
  9. microRNA analysis of neurons generated from patient-specific iPSCs
    Herbert M Lachman; Fiscal Year: 2013
    ..DGCR8 combines with Drosha to form the so-called "microprocessor," which produces miRNAs from long primary miRNAs...
  10. Role of miRNAs in Vascular Physiology
    Akiko Hata; Fiscal Year: 2012
    ..promoting the processing of primary transcripts of miR-21 (pri-miR-21) into precursor miR- 21 (pre-miR-21) by the Drosha microprocessor complex in the nucleus...
  11. Joan A Steitz; Fiscal Year: 2014
    ..and single-molecule approaches will complement functional studies of the core components of the Microprocessor-Drosha and DGCR8-to uncover the molecular basis of regulation of pri-microRNA processing in the nucleus of tumorigenic ..
  12. Tatsuya Kobayashi; Fiscal Year: 2016
    ..multiple processing steps;primary transcripts of miRNAs are first processed by the microprocessor containing Drosha and DGCR8 as critical components, and then by Dicer...
  13. Role of 11q23 Chromosome Abnormalities in the Causation of Acute Leukemia
    Carlo M Croce; Fiscal Year: 2013
    ..achievements we made recently include: 1) ALL1 fusion proteins bind together with the microRNAs processor enzyme Drosha to miR-191 to upregulate its expression, 2) knockdown of MEIS1 and HOX A10 or A9 in an ALL1-associated leukemic ..
  14. Circadian regulation of microRNA biogenesis and function
    Peter W Vanderklish; Fiscal Year: 2012
    ..2) "Antagomirs" and reporter constructs will be used to determine whether regulation of Drosha, Dicer and Ago2 by RBM3 involves direct effects on their translation, or feedback mechanism involving miRNAs that ..
  15. Piotr Sliz; Fiscal Year: 2016
    ..During this post- transcriptional stage, Lin28 can block both of the cleavage steps (by Drosha and Dicer), and promote degradation by recruiting a terminal uridylyl transferase, specifically for the let-7 ..
  16. Shizhen Emily Wang; Fiscal Year: 2016
    ..Breast cancer cells expressing various levels of the SMAD2/3 cofactors (i.e., SMAD4, Drosha and p68) will be examined for their dynamic regulation of SMAD2/3 function and TGF-2 effect...
  17. Karen M Lyons; Fiscal Year: 2016
    ..In Aim 3, we determine how Smad1-Drosha mediated miRNA maturation contributes to Smad1/5-dependent, Smad4-independent signaling through identification of ..
  18. Xiao Fan Wang; Fiscal Year: 2016
    ..3. Examine the regulatory mechanisms that modulate miRNA processing by the Drosha complex in response to hypoxia in GSCs...
  19. ALL Fusion Proteins: Associated with Multi-protein Complexes and Role in Transcri
    Carlo M Croce; Fiscal Year: 2010
    ..the upregulation is due to recruitment of ALL-1 fusion proteins, together with the general processor of microRNAs-Drosha, to the miR-191 locus...
  20. microRNA function in human embryonic stem cells division
    Junlin Qi; Fiscal Year: 2009
    ..reaching this goal, our preliminary data showed that hESCs lacking mature miRNAs due to knockdown (KD) of Dicer or Drosha have reduced cell cycle kinetics and delayed at both Gap-phases G1 and G2...
  21. Swathi Arur; Fiscal Year: 2016
    ..Among the crucial components are the RAS-extracellular regulated kinase (ERK) signaling pathway and Dicer and Drosha, essential mediators of small RNA biogenesis...
  22. CONFORMATIONAL VARIABILITY OF RNA
    JULI F FEIGON; Fiscal Year: 2010
    ..in higher organisms have been found to play key roles in miRNA and siRNA processing, as members of the Dicer and Drosha family of enzymes...
  23. Targeted Suppression of the Transcription Factor Sox9 in the Testis by Tissue-Spe
    Manjeet Rao; Fiscal Year: 2007
    ..In my approach, a shRNA molecule targeting Sox9 will be processed from a RhoxS Pp-driven precursor transcript by Drosha, a ubiquitously expressed RNase-lll enzyme that processes out naturally occurring miRNAs...
  24. Ultrafine Particle Exposure in Humans
    Mark Frampton; Fiscal Year: 2005
    ..Specific Aims: 1) Determine in nonsmokers the effects of inhalation of clean air and 50 pg/rn3 carbon UFP on endothelial function, leukocyte activation, and leukocyte oxidant production...
  25. Novel & Selective Small Molecular Inhibitors of Human Peptide Deformylase
    Hakim Djaballah; Fiscal Year: 2006
    ..Indeed, the likelihood that a compound interferes with both assays, and therefore behaves as a false positive in both assays, is vastly reduced compared to a strategy solely based on one technique. ..
  26. Mechanisms of TGFB Signal Transduction
    Akiko Hata; Fiscal Year: 2007
    ..These studies should provide important insights into TGFbeta-mediated regulation of gene expression, which is of direct relevance to both normal physiology and a variety of disease states. ..
  27. Role of BMPRII Mutants in the Pathogenesis of Pulmonary Hypertension
    Akiko Hata; Fiscal Year: 2009
    ....
  28. Analysis of mammalian odor receptors
    Stuart Firestein; Fiscal Year: 2009
    ..GPCRs are a major target of drugs and treatments for many neural, psychological and other pathologies. A deeper understanding of ORs will lead to more fundamental insights into GPGRs. ..
  29. Microarray Analysis of Olfactory Receptor Expression
    Stuart Firestein; Fiscal Year: 2008
    ..This will allow us to extend the bioinformatics work on the whole genome to an appreciation of dynamic gene expression and regulation. ..
  30. Real-time PCR expression profiling of microRNA
    Thomas Schmittgen; Fiscal Year: 2006
    ..The unparallel sensitivity and specificity of real-time PCR as applied to this new and exciting class of regulatory RNAs should propel the field into new directions not only in cancer but in other areas of human health. ..
  31. The Role of TIMP-1 in Uterine Physiology
    Warren Nothnick; Fiscal Year: 2005
    ..These studies will broaden our understanding on the role of TIMP-1 in uterine physiology and may help to develop new strategies to treat uterine disorders such as infertility, endometriosis and uterine cancer. ..
  32. Micro RNA Expression and Cancer
    Thomas Schmittgen; Fiscal Year: 2005
    ..Assays developed with this research may help to identify the function of a new class of genes in humans. ..
  33. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  34. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  35. Estrogen regulation of uterine microRNAs: Novel factors in MMP-9 regulation
    Warren Nothnick; Fiscal Year: 2008
    ..Enhancing our understanding on the regulation of MMP-9 expression may lead to the establishment of novel approaches to counteract mis-expression of this protease and the diseases associated with it. [unreadable] [unreadable] [unreadable]..
  36. Transcriptional Regulation of Oocyte-Specific Genes
    Jia Song; Fiscal Year: 2007
    ..Defining transcription complexes that regulate several oocyte gene promoters will create a paradigm in gene regulatory networks that orchestrates the development of oocytes and further our knowledge in reproductive biology. ..