FLJ10261

Summary

Gene Symbol: FLJ10261
Description: anoctamin 1
Alias: DOG1, ORAOV2, TAOS2, TMEM16A, anoctamin-1, Ca2+-activated Cl- channel, anoctamin 1, calcium activated chloride channel, calcium activated chloride channel, discovered on gastrointestinal stromal tumors protein 1, oral cancer overexpressed 2, transmembrane protein 16A (eight membrane-spanning domains), tumor-amplified and overexpressed sequence 2
Species: human

Top Publications

  1. doi Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors, including unusual subtypes
    Bernadette Liegl
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Am J Surg Pathol 33:437-46. 2009
  2. ncbi FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products
    Masuko Katoh
    M and M Medical BioInformatics, Narashino 275 0022, Japan
    Int J Oncol 22:1375-81. 2003
  3. ncbi Identification and characterization of TMEM16E and TMEM16F genes in silico
    Masuko Katoh
    M and M Medical BioInformatics, Narashino 275 0022, Japan
    Int J Oncol 24:1345-9. 2004
  4. doi TMEM16A confers receptor-activated calcium-dependent chloride conductance
    Young Duk Yang
    Sensory Research Center, CRI, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    Nature 455:1210-5. 2008
  5. doi TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity
    Antonella Caputo
    Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova 16148, Italy
    Science 322:590-4. 2008
  6. pmc Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels
    H Criss Hartzell
    Department of Cell Biology, Emory University School of Medicine, 615 Michael Street, 535 Whitehead Bldg, Atlanta, GA 30322, USA
    J Physiol 587:2127-39. 2009
  7. pmc Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract
    Pedro J Gomez-Pinilla
    Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA
    Am J Physiol Gastrointest Liver Physiol 296:G1370-81. 2009
  8. pmc TMEM16 proteins produce volume-regulated chloride currents that are reduced in mice lacking TMEM16A
    Joana Almaca
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    J Biol Chem 284:28571-8. 2009
  9. pmc Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles
    Sung Jin Hwang
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    J Physiol 587:4887-904. 2009
  10. pmc Regulation of TMEM16A chloride channel properties by alternative splicing
    Loretta Ferrera
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, Largo G Gaslini 5, 16147 Genova
    J Biol Chem 284:33360-8. 2009

Research Grants

  1. ROBERT VIRGIL STAHELIN; Fiscal Year: 2016
  2. LINDA M COLUMBUS; Fiscal Year: 2016
  3. Liviu Movileanu; Fiscal Year: 2016
  4. Structural and biochemical characterization of the OprD membrane protein family
    Bert van den Berg; Fiscal Year: 2011
  5. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
  6. David G Thanassi; Fiscal Year: 2016
  7. Natividad Ruiz; Fiscal Year: 2016
  8. David S Cafiso; Fiscal Year: 2016
  9. JAMIE KATHLEEN SCOTT; Fiscal Year: 2015
  10. PRASADARAO V NEMANI; Fiscal Year: 2014

Detail Information

Publications1366 found, 100 shown here

  1. doi Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors, including unusual subtypes
    Bernadette Liegl
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Am J Surg Pathol 33:437-46. 2009
    ..b>DOG1.1 is a recently described mouse monoclonal antibody reported to have superior sensitivity and specificity compared ..
  2. ncbi FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products
    Masuko Katoh
    M and M Medical BioInformatics, Narashino 275 0022, Japan
    Int J Oncol 22:1375-81. 2003
    ..1) derived from the uncharacterized FLJ10261 gene. Here, we characterized the FLJ10261 gene by using bioinformatics. NM_018043...
  3. ncbi Identification and characterization of TMEM16E and TMEM16F genes in silico
    Masuko Katoh
    M and M Medical BioInformatics, Narashino 275 0022, Japan
    Int J Oncol 24:1345-9. 2004
    TMEM16A (FLJ10261 or ORAOV2) gene within the CCND1-FGF4-EMS1 amplicon at human chromosome 11q13.3 encodes transmembrane protein homologous to TMEM16B (C12orf3), TMEM16C (C11orf25), and TMEM16D (FLJ34272)...
  4. doi TMEM16A confers receptor-activated calcium-dependent chloride conductance
    Young Duk Yang
    Sensory Research Center, CRI, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    Nature 455:1210-5. 2008
    ..Here we show that transmembrane protein 16A (TMEM16A, which we also call anoctamin 1 (ANO1)) is a bona fide Ca(2+)-activated chloride channel that is activated by intracellular Ca(2+) and Ca(2+)-..
  5. doi TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity
    Antonella Caputo
    Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova 16148, Italy
    Science 322:590-4. 2008
    ..Transfection of epithelial cells with specific small interfering RNA against each of these proteins shows that TMEM16A, a member of a family of putative plasma membrane proteins with unknown function, is associated with calcium-..
  6. pmc Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels
    H Criss Hartzell
    Department of Cell Biology, Emory University School of Medicine, 615 Michael Street, 535 Whitehead Bldg, Atlanta, GA 30322, USA
    J Physiol 587:2127-39. 2009
    ..Some members of this family are up-regulated in a number of tumours and functional deficiency in others is linked to developmental defects...
  7. pmc Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract
    Pedro J Gomez-Pinilla
    Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA
    Am J Physiol Gastrointest Liver Physiol 296:G1370-81. 2009
    ..No other molecular marker currently exists to independently identify ICC. The expression of ANO1 (DOG1, TMEM16A), a Ca(2+)-activated Cl(-) channel, in gastrointestinal stromal tumors suggests it may be useful as an ICC marker...
  8. pmc TMEM16 proteins produce volume-regulated chloride currents that are reduced in mice lacking TMEM16A
    Joana Almaca
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    J Biol Chem 284:28571-8. 2009
    ..We show that the Ca(2+)-activated Cl(-) channel TMEM16A together with other TMEM16 proteins are activated by cell swelling through an autocrine mechanism that involves ..
  9. pmc Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles
    Sung Jin Hwang
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    J Physiol 587:4887-904. 2009
    ..We investigated the expression and function of a new class of CaCC, anoctamin 1 (ANO1), encoded by Tmem16a, which was discovered to be highly expressed in ICC in a microarray screen...
  10. pmc Regulation of TMEM16A chloride channel properties by alternative splicing
    Loretta Ferrera
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, Largo G Gaslini 5, 16147 Genova
    J Biol Chem 284:33360-8. 2009
    Expression of TMEM16A protein is associated with the activity of Ca(2+)-activated Cl(-) channels. TMEM16A primary transcript undergoes alternative splicing. thus resulting in the generation of multiple isoforms...
  11. pmc Identification and functional characterization of TMEM16A, a Ca2+-activated Cl- channel activated by extracellular nucleotides, in biliary epithelium
    Amal K Dutta
    Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9063, USA
    J Biol Chem 286:766-76. 2011
    ..The present studies in human, mouse, and rat BECs provide evidence that TMEM16A is the operative channel and contributes to Ca(2+)-activated Cl(-) secretion in response to extracellular ..
  12. pmc TMEM16A inhibitors reveal TMEM16A as a minor component of calcium-activated chloride channel conductance in airway and intestinal epithelial cells
    Wan Namkung
    Department of Medicine, University of California, San Francisco, California 94143, USA
    J Biol Chem 286:2365-74. 2011
    b>TMEM16A (ANO1) functions as a calcium-activated chloride channel (CaCC). We developed pharmacological tools to investigate the contribution of TMEM16A to CaCC conductance in human airway and intestinal epithelial cells...
  13. pmc Altered expression of Ano1 variants in human diabetic gastroparesis
    Amelia Mazzone
    Enteric Neuroscience Program, Division of Gastroenterology and Hepatology, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Biol Chem 286:13393-403. 2011
    ..Changes in Ano1 expression in ICC may directly contribute to diabetic gastroparesis...
  14. doi DOG1 antibody is a highly sensitive and specific marker for gastrointestinal stromal tumors in cytology cell blocks
    Dick G Hwang
    Dept of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Am J Clin Pathol 135:448-53. 2011
    ..b>DOG1 has been found to be a specific and sensitive marker for GISTs on surgical material...
  15. pmc A minimal isoform of the TMEM16A protein associated with chloride channel activity
    Loretta Ferrera
    Laboratorio di Genetica Molecolare, Largo Gerolamo Gaslini, Istituto Giannina Gaslini, 16147 Genova, Italy
    Biochim Biophys Acta 1808:2214-23. 2011
    b>TMEM16A protein, also known as anoctamin-1, has been recently identified as an essential component of Ca(2+)-activated Cl(-) channels. We previously reported the existence of different TMEM16A isoforms generated by alternative splicing...
  16. pmc Ano1 as a regulator of proliferation
    Jennifer E Stanich
    Enteric Neuroscience Program, Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota 55905, USA
    Am J Physiol Gastrointest Liver Physiol 301:G1044-51. 2011
    ..These data led us to conclude that Ano1 regulates proliferation at the G(1)/S transition of the cell cycle and may play a role in tumorigenesis...
  17. doi The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels
    Paolo Scudieri
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, 16147 Genova, Italy
    Exp Physiol 97:177-83. 2012
    ..Recently, the question of the molecular identity of CaCCs has apparently been resolved with the identification of TMEM16A protein (also known as anoctamin-1)...
  18. doi CFTR and TMEM16A are separate but functionally related Cl- channels
    Jiraporn Ousingsawat
    Institut fur Physiologie, Universitat Regensburg, Universitätsstraße 31, Regensburg, Germany
    Cell Physiol Biochem 28:715-24. 2011
    ..Recent findings showing that TMEM16A forms the essential part of CaCC, prompted us to examine whether CFTR controls TMEM16A...
  19. doi Gastrointestinal stromal tumors (GISTs): CD117, DOG-1 and PKCθ expression. Is there any advantage in using several markers?
    M J Ríos-Moreno
    Servicio de Anatomia Patologica, Hospital Universitario Virgen Macarena, Seville, Spain
    Pathol Res Pract 208:74-81. 2012
    ..Expression of CD117, DOG1 and PKCθ was investigated immunohistochemically in a series of 99 paraffin-embedded GISTs in order to determine ..
  20. doi DOG1: a novel marker of salivary acinar and intercalated duct differentiation
    Jacinthe Chenevert
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Mod Pathol 25:919-29. 2012
    Anoctamin-1 (ANO1) (DOG1, TMEM16a) is a calcium-activated chloride channel initially described in gastrointestinal stromal tumors, but now known to be expressed in a variety of normal and tumor tissues including salivary tissue in murine ..
  21. pmc TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression
    Umamaheswar Duvvuri
    Department of Otolaryngology, University of Pittsburgh Medical Center, University of Pittsburgh School of Medicine and Magee Women s Research Institute, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 72:3270-81. 2012
    Frequent gene amplification of the receptor-activated calcium-dependent chloride channel TMEM16A (TAOS2 or ANO1) has been reported in several malignancies. However, its involvement in human tumorigenesis has not been previously studied...
  22. pmc Anoctamin 1 (Tmem16A) Ca2+-activated chloride channel stoichiometrically interacts with an ezrin-radixin-moesin network
    Patricia Perez-Cornejo
    Physiology Department, School of Medicine, Universidad Autonoma de San Luis Potosi, San Luis Potosi, SLP 78210, Mexico
    Proc Natl Acad Sci U S A 109:10376-81. 2012
    The newly discovered Ca(2+)-activated Cl(-) channel (CaCC), Anoctamin 1 (Ano1 or TMEM16A), has been implicated in vital physiological functions including epithelial fluid secretion, gut motility, and smooth muscle tone...
  23. pmc Enhanced expression of ANO1 in head and neck squamous cell carcinoma causes cell migration and correlates with poor prognosis
    Christian Ruiz
    Institute for Pathology, University Hospital Basel, Basel, Switzerland
    PLoS ONE 7:e43265. 2012
    ..Ano1 (Dog1) is an established and sensitive marker for the diagnosis of gastrointestinal stromal tumors (GIST) and has ..
  24. doi Anoctamins are a family of Ca2+-activated Cl- channels
    Yuemin Tian
    Institut fur Physiologie, Universitat Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany
    J Cell Sci 125:4991-8. 2012
    b>Anoctamin 1 (Ano1; TMEM16A) and anoctamin 2 (Ano2; TMEM16B) are novel Cl(-) channels transiently activated by an increase in intracellular Ca(2+)...
  25. pmc Potent vasorelaxant activity of the TMEM16A inhibitor T16A(inh) -A01
    Alison J Davis
    Pharmacology and Cell Physiology Research Group, Division of Biomedical Sciences, St George s, University of London, London, UK
    Br J Pharmacol 168:773-84. 2013
    T16A(inh) -A01 is a recently identified inhibitor of the calcium-activated chloride channel TMEM16A. The aim of this study was to test the efficacy of T16A(inh) -A01 for inhibition of calcium-activated chloride channels in vascular ..
  26. pmc Control of TMEM16A by INO-4995 and other inositolphosphates
    Yuemin Tian
    Institut fur Physiologie, Universitat Regensburg, Germany
    Br J Pharmacol 168:253-65. 2013
    ..Cl(-) secretion (CaCC) in airways and other tissues is due to activation of the Cl(-) channel TMEM16A (anoctamin 1)...
  27. pmc Increased TMEM16A-encoded calcium-activated chloride channel activity is associated with pulmonary hypertension
    Abigail S Forrest
    Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 0573, USA
    Am J Physiol Cell Physiol 303:C1229-43. 2012
    ..analysis revealed that the alterations in I(Cl(Ca)) were accompanied by parallel changes in the expression of TMEM16A, a gene recently shown to encode for Cl(Ca) channels...
  28. pmc Calcium-activated chloride channel ANO1 promotes breast cancer progression by activating EGFR and CAMK signaling
    Adrian Britschgi
    Friedrich Miescher Institute for Biomedical Research, CH 4058 Basel, Switzerland
    Proc Natl Acad Sci U S A 110:E1026-34. 2013
    The calcium-activated chloride channel anoctamin 1 (ANO1) is located within the 11q13 amplicon, one of the most frequently amplified chromosomal regions in human cancer, but its functional role in tumorigenesis has remained unclear...
  29. doi TMEM16A-TMEM16B chimaeras to investigate the structure-function relationship of calcium-activated chloride channels
    Paolo Scudieri
    U O C Genetica Medica, Istituto Giannina Gaslini, Via Gerolamo Gaslini 5, 16147 Genova, Italy
    Biochem J 452:443-55. 2013
    b>TMEM16A and TMEM16B proteins are CaCCs (Ca2+-activated Cl- channels) with eight putative transmembrane segments...
  30. pmc DOG1 regulates growth and IGFBP5 in gastrointestinal stromal tumors
    Susanne Simon
    Sarcoma Center, Departments of Medical Oncology, Pathology and Neuropathology, Trauma and Orthopedic Surgery, Visceral and Transplant Surgery, West German Cancer Center, University Duisburg Essen, University Hospital Essen, Germany
    Cancer Res 73:3661-70. 2013
    ..The calcium-dependent chloride channel DOG1 (ANO1/TMEM16A), which is strongly and specifically expressed in GIST, is used as a diagnostic marker to differentiate GIST from ..
  31. pmc Identification of a dimerization domain in the TMEM16A calcium-activated chloride channel (CaCC)
    Jason Tien
    Department of Physiology, and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 110:6352-7. 2013
    Transmembrane proteins with unknown function 16 (TMEM16A) is a calcium-activated chloride channel (CaCC) important for neuronal, exocrine, and smooth muscle functions...
  32. pmc TMEM16A alternative splicing coordination in breast cancer
    Ifeoma Ubby
    Human Molecular Genetics, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
    Mol Cancer 12:75. 2013
    b>TMEM16A, also known as Anoctamin-1, is a calcium-activated chloride channel gene overexpressed in many tumors...
  33. pmc Epidermal growth factor chronically upregulates Ca(2+)-dependent Cl(-) conductance and TMEM16A expression in intestinal epithelial cells
    Magdalena S Mroz
    Department of Molecular Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland
    J Physiol 590:1907-20. 2012
    ..01). EGF-induced increases in CaCC currents were abolished by the transmembrane protein 16A (TMEM16A) inhibitor, T16A(inh)-A01 (10 μm)...
  34. ncbi Characterization of the molecular mode of action of the sulfonylurea, glimepiride, at adipocytes
    G Muller
    Hoechst AG Frankfurt, Germany
    Horm Metab Res 28:469-87. 1996
    ....
  35. pmc Calcium-dependence of histamine- and carbachol-induced inositol phosphate formation in human U373 MG astrocytoma cells: comparison with HeLa cells and brain slices
    J A Arias-Montaño
    Department of Pharmacology, University of Cambridge
    Br J Pharmacol 111:598-608. 1994
    ..Whether U373 MG cells will be of use as a model system for the apparent Ca2+-entry component observed in guinea-pig or rat brain slices remains to be established...
  36. pmc Reversible binding kinetics of a cytoskeletal protein at the erythrocyte submembrane
    A L Stout
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055
    Biophys J 67:1324-34. 1994
    ....
  37. ncbi Stimulation of calmodulin binding to skeletal muscle membrane proteins by 1,25-dihydroxy-vitamin D3
    V Massheimer
    , Universidad Nacional del Sur, , Argentina
    Z Naturforsch C 45:663-70. 1990
    ..These changes may play a role in the stimulation of muscle Ca uptake by the sterol...
  38. ncbi Glucose induces amphiphilic to hydrophilic conversion of a subset of glycosyl-phosphatidylinositol-anchored ectoproteins in yeast
    W Bandlow
    , , Federal Republic of Germany
    Arch Biochem Biophys 324:300-16. 1995
    ..GPI anchor cleavage in yeast appears to resemble strikingly the situation in insulin-responsive adipocytes subsequently to stimulation by insulin of glucose transport into these cells...
  39. pmc ECL cell morphology
    D Chen
    Department of Pharmacology, University of Lund, Sweden
    Yale J Biol Med 71:217-31. 1998
    ..Subsequent lysosomal degradation of the vacuoles and their contents resulted in the development of lipofuscin bodies...
  40. ncbi [Immunodeficiency and carcinogenesis in patients with chronic active Epstein-Barr virus infection]
    S Nagafuchi
    First Department of Internal Medicine, Faculty of Medicine, Kyushu University
    Kansenshogaku Zasshi 71:56-64. 1997
    ..Further studies are required to clarify the exact mechanisms responsible for the development of CAEBV...
  41. ncbi Identification of peroxisomal membrane ghosts with an epitope-tagged integral membrane protein in yeast mutants lacking peroxisomes
    P E Purdue
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY 10029, USA
    Yeast 11:1045-60. 1995
    ..Gold-labelled membranes were clearly visible in both mutants: in peb2 the labelled membrane vesicles were generally much smaller than those in peb4, which resembled normal peroxisomes in size...
  42. ncbi Aqueous two-phase systems containing self-associating block copolymers. Partitioning of hydrophilic and hydrophobic biomolecules
    M Svensson
    Department of Physical Chemistry 1, Lund University, Sweden
    J Chromatogr A 839:71-83. 1999
    ..i) At the higher temperature, insertion of tryptophan-rich peptides increased the partitioning to the Pluronic phase. (ii) At the lower temperature, lower values of K were observed for ZT2 than for ZT1...
  43. ncbi Epidermal growth factor receptor and estrogen and progesterone receptors in breast cancers of premenopausal and postmenopausal patients
    E Skasko
    Endocrinological Laboratory, Maria Sklodowska-Curie Memorial Cancer Center, Warsaw, Poland
    Tumori 80:56-60. 1994
    ....
  44. pmc Temperature-regulated protein synthesis by Leptospira interrogans
    J E Nally
    Department of Veterinary Medicine, University of Kentucky, Lexington, Kentucky 40546-0099, USA
    Infect Immun 69:400-4. 2001
    ....
  45. pmc Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium
    C R Gentry-Weeks
    Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892
    J Bacteriol 174:7729-42. 1992
    ..avium 197 resulted in colonization of the trachea and thymus with B. avium 197...
  46. pmc A pertussis toxin-sensitive mechanism of endothelin action in porcine coronary artery smooth muscle
    Y Kasuya
    Department of Pharmacology, University of Tsukuba, Ibaraki, Japan
    Br J Pharmacol 107:456-62. 1992
    ..It is concluded that the action of ET-1 in porcine coronary artery is mediated via two distinct signal transduction pathways, which are coupled to PT-sensitive and PT-insensitive GTP-binding proteins, respectively...
  47. ncbi Isolation, chemical characterization, and immunohistochemical localization of a protein from the basolateral plasma membrane of the rat intestinal absorptive cell
    H Schiechl
    Institute of Histology and Embryology, University of Graz, School of Medicine, Austria
    Z Naturforsch C 44:289-95. 1989
    ..Immunofluorescence labelling of rat small intestinal sections with this serum showed that Prot 17 was not a BLM-specific protein. It occurred in both plasma membrane domains of the intestinal absorptive cell...
  48. ncbi Killer-toxin-resistant kre12 mutants of Saccharomyces cerevisiae: genetic and biochemical evidence for a secondary K1 membrane receptor
    M J Schmitt
    , , D-55099 Mainz, Germany
    Arch Microbiol 164:435-43. 1995
    ..A model for K1 killer toxin action is presented in which the gene product of KRE12 functions in vivo as a K1 docking protein, facilitating toxin binding to the membrane and subsequent ion channel formation...
  49. ncbi [Studies on immune response in mouse model of experimental Chlamydia trachomatis intrauterine infections]
    T Satoh
    Department of Urology, School of Medicine, Sapporo Medical University
    Kansenshogaku Zasshi 68:304-13. 1994
    ..It was also surmised that, at the sites of infection, cells belonging to the T-cell lineage, especially CD8+ cells, play an important role in the host's defense mechanism against the infection by C. trachomatis...
  50. pmc Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles
    M J Schnell
    Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 93:11359-65. 1996
    ..Recombinant VSVs carrying foreign antigens on the surface of the virus particle may be useful for viral targeting, membrane protein purification, and for generation of immune responses...
  51. pmc Interaction of a peptide model of a hydrophobic transmembrane alpha-helical segment of a membrane protein with phosphatidylethanolamine bilayers: differential scanning calorimetric and Fourier transform infrared spectroscopic studies
    Y P Zhang
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biophys J 68:847-57. 1995
    ....
  52. ncbi Inducible membranes in yeast: relation to the unfolded-protein-response pathway
    R Menzel
    Department of Cell Biology, , Berlin Buch, Germany
    Yeast 13:1211-29. 1997
    ..We suggest that Ire1p-mediated KAR2 induction is, in the case of cytochrome P450 52A3 overproduction, a process which follows on ER proliferation, thereby monitoring the increase of ER size and adjusting the level of Kar2p accordingly...
  53. ncbi [Molecular analysis of peroxisomal disorders]
    N Shimozawa
    Department of Pediatrics, Gifu University School of Medicine
    No To Hattatsu 30:128-33. 1998
    ..The mouse model should facilitate researches on PBD and ALD, especially those on regulatory factors of their phenotypic heterogeneity and on new therapeutic approaches...
  54. ncbi Synaptophysin. A widespread constituent of small neuroendocrine vesicles and a new tool in tumor diagnosis
    B Wiedenmann
    Department of Internal Medicine, Steglitz Medical Center, Free University of Berlin, Germany
    Acta Oncol 30:435-40. 1991
    ....
  55. ncbi Isolation and characterization of Pas2p, a peroxisomal membrane protein essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris
    E A Wiemer
    Department of Biology, University of California at San Diego, La Jolla, California 92093 0322, USA
    J Biol Chem 271:18973-80. 1996
    ..The role of peroxisomal membrane targeting signals and transmembrane domains in anchoring PMPs to the peroxisomal membrane is discussed...
  56. ncbi Bicarbonate permeability and immunological evidence for an anion exchanger-like protein in the red blood cells of the sea lamprey, Petromyzon marinus
    B A Cameron
    Department of Biology, Queen s University, Kingston, Ontario, Canada
    J Comp Physiol B 166:197-204. 1996
    ..Therefore, lamprey red blood cells appear to possess an AE1-like protein that may be physiologically different than that in most other vertebrates...
  57. pmc Analysis of protein localization by use of gene fusions with complementary properties
    C Manoil
    Department of Genetics, University of Washington, Seattle 98195
    J Bacteriol 172:1035-42. 1990
    ..In addition, fusion switching makes it possible to generate gene fusions whose properties should facilitate the isolation of mutants defective in the export or membrane anchoring of different cell envelope proteins...
  58. ncbi Stimulation of myoblast membrane protein synthesis by 25-hydroxy-vitamin D3
    T Bellido
    , Universidad Nacional del Sur, , Argentina
    Z Naturforsch C 44:807-12. 1989
    ....
  59. pmc Activation of protein kinase C zeta induces serine phosphorylation of VAMP2 in the GLUT4 compartment and increases glucose transport in skeletal muscle
    L Braiman
    Faculty of Life Sciences, Gonda-Goldschmied Center, Bar-Ilan University, Ramat-Gan 52900, Israel
    Mol Cell Biol 21:7852-61. 2001
    ..These results demonstrate that PKC zeta regulates insulin-stimulated GLUT4 translocation and glucose transport through the unique colocalization of this isoform with the GLUT4 compartments...
  60. ncbi [Detection of Chlamydia trachomatis from urethral swab in male urethritis by polymerase chain reaction]
    T Deguchi
    Department of Urology, Gifu University School of Medicine
    Kansenshogaku Zasshi 65:1555-9. 1991
    ..In two specimens concluded s negative by culture method, amplified C. trachomatis DNA were detected by PCR. Thus, the PCR would be a more simple and sensitive method for detection of C. trachomatis, compared with the culture method...
  61. ncbi [Detection of chlamydial antibodies by immunoblotting technique--in comparison of three commercially available test kits and micro-IF]
    T Komoda
    Department of Public Health, Kyorin University School of Health Sciences, Japan
    Kansenshogaku Zasshi 70:232-8. 1996
    ..This indicates that W-B technique with MOMP and other protein bands (40-62 KDa) as marker for positive reaction is a useful method for detection of chlamydial antibodies...
  62. pmc Effective formation of major histocompatibility complex class II-peptide complexes from endogenous antigen by thyroid epithelial cells
    R Maile
    Division of Medicine, University of Bristol, Bristol Royal Infirmary, Bristol, UK
    Immunology 99:367-74. 2000
    ..These results provide the most direct evidence to date that TEC can form MHC class II-peptide complexes derived from self-antigen in sufficient quantities to activate T cells...
  63. ncbi Glutathione peroxidase, thioredoxin, and membrane protein changes in erythrocytes predict ribavirin-induced anemia
    Ignazio Grattagliano
    Section of Internal Medicine, Department of Internal Medicine and Public Medicine, University of Bari, Bari, Italy
    Clin Pharmacol Ther 78:422-32. 2005
    ..Low erythrocyte membrane protein sulfhydril concentrations are a risk factor for ribavirin-induced anemia. We further studied the role of oxidative stress and erythrocyte membrane alterations in ribavirin-induced anemia...
  64. pmc Effect of Ca2+ binding on the profile structure of the sarcoplasmic reticulum membrane using time-resolved x-ray diffraction
    L J DeLong
    Department of Chemistry, University of Pennsylvania, Philadelphia 19104
    Biophys J 64:1750-9. 1993
    ....
  65. ncbi [Comparison of polymerase chain reaction and IDEIA Chlamydia in detection of Chlamydia trachomatis from first-voided urine of male urethritis patients]
    H Komeda
    Department of Urology, Gifu University School of Medicine
    Kansenshogaku Zasshi 66:1473-8. 1992
    ..6% (65/68) respectively, resulting in a high overall coincidence rate at 95%. Thus, the improved method with PCR using FVU as a specimen is proved to be a useful, non-invasive diagnostic tool for diagnosis of chlamydial urethritis...
  66. ncbi [Comparison of polymerase chain reaction and enzyme immunoassay (Chlamydiazyme) in detection of Chlamydia trachomatis from male urethritis]
    T Deguchi
    Department of Urology, Gifu University School of Medicine
    Kansenshogaku Zasshi 66:172-6. 1992
    ..9% (29/33). The overall coincidence rate between the two methods was high (94.6%). Thus, the PCR method was more sensitive than Chlamydiazyme for detection of C. trachomatis and specific for diagnosis of chlamydial urethritis...
  67. ncbi Selective blockade of voltage-gated potassium channels reduces inflammatory bone resorption in experimental periodontal disease
    Paloma Valverde
    Department of Oral and Developmental Biology, Harvard Medical School and Harvard School of Dental Medicine, Boston, Massachusetts, USA
    J Bone Miner Res 19:155-64. 2004
    ..This study suggests a plausible therapeutic approach for inflammatory bone resorption by targeting Kv1.3...
  68. pmc Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility
    J D Corbett
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115
    Biophys J 66:25-30. 1994
    ..This effect has important implications for the ability of the fluorescence photobleaching recovery technique to report accurate lateral mobilities of cell membrane molecules under hypoxic conditions...
  69. ncbi Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus
    S I Yoshimura
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    J Cell Sci 114:4105-15. 2001
    ..These results strongly suggested that GM130 and GRASP65 are directly targeted to the Golgi membrane without initial assembly on the ER and subsequent vesicular transport to the Golgi apparatus...
  70. pmc Hydration at the membrane protein-lipid interface
    C Ho
    Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107
    Biophys J 63:897-902. 1992
    ..Altered hydration at the protein-lipid interface could affect conformation, thereby offering a new route by which membrane protein functioning may be modified...
  71. ncbi A method for determining the in vivo topology of yeast polytopic membrane proteins demonstrates that Gap1p fully integrates into the membrane independently of Shr3p
    C F Gilstring
    Ludwig Institute for Cancer Research, Box 240, S 171 77 Stockholm, Sweden
    J Biol Chem 275:31488-95. 2000
    ..Gap1p exhibited the same membrane topology in strains lacking Shr3p; therefore, Gap1p fully integrates into the ER membrane independently of this permease-specific packaging chaperone...
  72. ncbi Protein sorting between mitochondrial outer and inner membranes. Insertion of an outer membrane protein into the inner membrane
    J M Li
    Department of Biochemistry, McGill University, Montreal, Canada
    Biochim Biophys Acta 1106:233-41. 1992
    ..The significance of these findings for sorting of proteins between the mitochondrial inner and outer membranes is discussed...
  73. ncbi Development of a dual membrane protein reporter system using sodium iodide symporter and mutant dopamine D2 receptor transgenes
    Do Won Hwang
    Programs in Neuroscience, Seoul National University, Seoul, Korea
    J Nucl Med 48:588-95. 2007
    ..Human sodium iodide symporter (hNIS) and mutant dopamine D(2) receptor (D(2)R) transgenes were chosen considering their complementarity...
  74. ncbi [The effect of temperature on the growth and lipopolysaccharide production of gram-negative bacteria]
    C Weber Frick
    ,
    Zentralbl Bakteriol Mikrobiol Hyg B 187:56-69. 1988
    ..In the use of the LAL-tests methods for the assessment of the bacterial load of foods with gram negative bacteria, it is necessary, especially for cold stored products, to test for and determine the LPS-cfu relationship beforehand...
  75. pmc Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro
    M J Kuehn
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA
    J Cell Biol 135:585-95. 1996
    ..Our data suggest that specific ancillary proteins in the ER membrane recruit subsets of integral membrane protein cargo into COPII transport vesicles...
  76. ncbi Porin of Rhodobacter capsulatus: biochemical and functional characterization
    D Woitzik
    , Mikrobiologie, , Freiburg, Bundesrepublik Deutschland
    Z Naturforsch C 45:576-82. 1990
    ..0, was obtained on isoelectric focusing. Gas-phase sequencing of the 23 N-terminal residues revealed Glu-Val-Lys-Leu-Ser-Gly-Asp-Ala-Arg-Met-Gly-Val-Met-Tyr-Asn-Gly-Asp-Asp- X-Asn- Phe-Ser-Ser...
  77. ncbi Overview on human breast cancer with focus on prognostic and predictive factors with special attention on the tumour suppressor gene p53
    T Norberg
    Department of Oncology, Univ. of Uppsala, Akademiska sjukhuset, Sweden
    Acta Oncol 35:96-102. 1996
    ..In this overview we shortly describe the above mentioned prognostic factors with major focus on the tumour suppressor gene p53 and its prognostic value and potential predictive value...
  78. ncbi A stretch of positively charged amino acids at the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into the peroxisomal membrane
    R J Baerends
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Biol Chem 275:9986-95. 2000
    ..g. Pex10p and Pex14p, were also diminished, suggesting that Pex3p maybe involved in the recruitment or stabilization of these proteins (in the membrane)...
  79. ncbi Differential distribution of vesicle associated membrane protein isoforms in the mouse retina
    David M Sherry
    College of Optometry, University of Houston, Houston, TX 77204 2020, USA
    Mol Vis 9:673-88. 2003
    ..Two VAMP isoforms have been identified that are considered to be synaptic, VAMP-1 and VAMP-2, however their distributions among the various synapses in the mammalian retina have not been characterized...
  80. ncbi Specific interaction of bovine IgG1 and IgG2 subclasses with different chlamydial antigens
    N Schmeer
    Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge
    Zentralbl Bakteriol Mikrobiol Hyg A 266:305-15. 1987
    ..The results obtained by this method may have implications for the production of a subunit vaccine as well as for serodiagnostic purposes...
  81. ncbi An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes
    L van Dyck
    , , Louvain la Neuve, Belgium
    Yeast 13:171-6. 1997
    ..The segment also contains tRNA genes for Asn, Arg and Ile as well as sigma element and two solo deltas. ORFs and genetic elements are named according to a preliminary working nomenclature...
  82. ncbi Binding of a thromboxane A2 (TXA2)/prostaglandin H2 (PGH2) receptor antagonist to rabbit and pig heart membrane protein
    B Lasserre
    , Institut de Physiologie, Toulouse, France
    Prostaglandins Leukot Essent Fatty Acids 47:153-7. 1992
    ....
  83. ncbi UT-A2: a 55-kDa urea transporter in thin descending limb whose abundance is regulated by vasopressin
    J B Wade
    Department of Physiology, Univ. of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 278:F52-62. 2000
    ..These results indicate that UT-A2 is expressed as a 55-kDa protein in portions of the thin descending limbs of Henle's loop and that the abundance of this protein is strongly upregulated by vasopressin...
  84. ncbi Deviant Pex3p levels affect normal peroxisome formation in Hansenula polymorpha: high steady-state levels of the protein fully abolish matrix protein import
    R J Baerends
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Yeast 13:1437-48. 1997
    ..The implications of our results on PEX3 gene regulation and functioning of the peroxisomal matrix protein import machinery in H. polymorpha are discussed...
  85. ncbi Expression of two membrane fusion proteins, synaptosome-associated protein of 25 kDa and vesicle-associated membrane protein, in choroid plexus epithelium
    I Chung
    Department of Clinical Neurosciences, Brown University School of Medicine, Aldrich Building, Room 403, 593 Eddy Street, Providence, RI 02903, USA
    Neuroscience 116:349-57. 2003
    ....
  86. ncbi [The clinical significance of specific IgA antibodies in local secretions in cases with chlamydial urogenital infections--comparison with serum antibodies and analysis of antigen specificity by immunoblotting assay]
    K Hayashi
    Department of Urology, Sapporo Medical College
    Kansenshogaku Zasshi 65:1430-45. 1991
    ..Examination of IgA antibody in local secretions was considered to be useful for making a correct diagnosis even in cases who were suspected to have C. trachomatis infection but showed negative antigen...
  87. ncbi KTR2: a new member of the KRE2 mannosyltransferase gene family
    M Lussier
    Department of Biology, McGill University, , , Canada
    Yeast 9:1057-63. 1993
    ..A null mutation in KTR2 leads to partial resistance to killer toxin and hints that KTR2, which encodes a putative mannosyltransferase, is involved in extracellular matrix assembly...
  88. ncbi [Local immunization against P. aeruginosa with an outer-membrane-protein vaccine]
    T Grundmann
    , Medizinische Hochschule Hannover
    Pneumologie 45:924-7. 1991
    ..Thus, mucosal immunization with OMP vaccines might hinder initial colonisation of the lungs with P. aeruginosa...
  89. doi Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis
    Duraipandian Thavaselvam
    Division of Microbiology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India
    J Med Microbiol 59:421-8. 2010
    ..This ELISA system in microtitre plates and a dot-blot format will be useful for the rapid screening of large numbers of samples for the diagnosis of human brucellosis in endemic areas...
  90. doi Identification of Yersinia pestis and Escherichia coli strains by whole cell and outer membrane protein extracts with mass spectrometry-based proteomics
    Rabih E Jabbour
    SAIC, Aberdeen Proving Ground, Maryland 21010, US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland 21010 5424, USA
    J Proteome Res 9:3647-55. 2010
    ....
  91. ncbi Studies on clonal assignment of urinary Escherichia coli O1:K1 strains
    W Nimmich
    , , DDR
    Zentralbl Bakteriol Mikrobiol Hyg A 266:323-9. 1987
    ..6 Md was found in all of these isolates. Colicin production was found to be useful for clonal assignment only in two thirds of the F9 and not in the F11 strains. Some strains with identical properties seem to be of epidemic importance...
  92. ncbi Reduction of red cell glucose transporter intrinsic activity in diabetes running
    R J Comi
    Department of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire
    Horm Metab Res 26:26-32. 1994
    ..This may be due to alterations in the transporter or its membrane environment...
  93. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark
    Biochem Biophys Res Commun 280:172-6. 2001
    ..Thus, these results strongly suggest a critical role of SNAREs in regulated exocytosis in eosinophils...
  94. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University of Aarhus, Aarhus C, DK-8000, Denmark
    Biochem Biophys Res Commun 282:194-9. 2001
    ..Thus, these results strongly suggest a critical role of SNAREs in regulated exocytosis in eosinophils...
  95. ncbi The story of the discovery of aquaporins: convergent evolution of ideas--but who got there first?
    P W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Cell Mol Biol (Noisy-le-grand) 52:2-5. 2006
    ....
  96. ncbi Reduced calcium binding capacity is an intrinsic abnormality of red blood cell membrane in spontaneously hypertensive rats
    M Cirillo
    Institute of Internal Medicine and Metabolic Diseases, 2nd Medical School, University of Naples, Italy
    Am J Hypertens 3:570-2. 1990
    ..The results support the concept of a reduced membrane Ca binding capacity in rat genetic hypertension: this abnormality is detectable only in membrane preparations with reduced cytoskeletal content...
  97. ncbi Functional analysis of vaccinia virus B5R protein: role of the cytoplasmic tail
    M M Lorenzo
    , Madrid, Valdeolmos, 28130, Spain
    Virology 252:450-7. 1998
    ....
  98. ncbi Characterization of further association of the trimeric membrane protein porin by low-angle laser-light scattering photometry coupled with high-performance gel chromatography
    Y Watanabe
    Institute for Protein Research, Osaka University, Japan
    J Chromatogr A 653:241-6. 1993
    ..The addition of lipopolysaccharide, another component of the outer membrane, and subsequent dialysis induced association of the trimer, the product containing an appreciable amount of the hexamer...
  99. ncbi Serum antibodies specific to CD outer membrane protein of Moraxella catarrhalis, P6 outer membrane protein of non-typeable Haemophilus influenzae and capsular polysaccharides of Streptococcus pneumoniae in children with otitis media with effusion
    Y Harabuchi
    Department of Otolaryngology, Sapporo Medical University School of Medicine, Japan
    Acta Otolaryngol 118:826-32. 1998
    ..These data suggest that persistence and/or recurrence of OME may be due to an insufficient serum antibody response to middle ear pathogens in young children...
  100. ncbi [Plasticity and remodeling of the myocardium in hypertensive patients. The inotropic effect]
    B Swynghedauw
    U 127-INSERM, , Paris
    Arch Mal Coeur Vaiss 84:93-7. 1991
    ..The therapeutic inotropic effect is obviously deleterious at fibre level but it may, on the contrary, be economically beneficial from the thermodynamic point of view to the heart as a whole...
  101. ncbi [Polypeptide profiles of Chlamydia pneumoniae strains isolated from Japan and western blot analysis using patients' sera]
    T Yamazaki
    Department of Pediatrics, Saitama Medical School
    Kansenshogaku Zasshi 67:535-40. 1993
    ..pneumoniae. However, recognition to MOMP of C. pneumoniae seems to be different among patients. This result might suggest the presence of subtype among C. pneumoniae strains...

Research Grants101 found, 100 shown here

  1. ROBERT VIRGIL STAHELIN; Fiscal Year: 2016
    ....
  2. LINDA M COLUMBUS; Fiscal Year: 2016
    ..By gaining an understanding of these molecular interactions, insights into the rational design of novel antibiotics, vaccine development, and targeting of liposome pharmaceutical carriers will be obtained. ..
  3. Liviu Movileanu; Fiscal Year: 2016
    ..They will also provide a new generation of research tools in nanomedicine for examining the details of complex recognition events in a quantitative manner. ..
  4. Structural and biochemical characterization of the OprD membrane protein family
    Bert van den Berg; Fiscal Year: 2011
    ..Besides being important from a fundamental point of view, such knowledge could also be used by pharmaceutical companies to design better drugs against disease-causing bacteria. ..
  5. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
    ..Ultimately, the studies performed in this Program will lead to fundamental insights that should be important for understanding and treating or preventing various acquired and inherited disorders of the liver. ..
  6. David G Thanassi; Fiscal Year: 2016
    ..This application will provide insights into mechanisms of protein secretion, organelle biogenesis, and the assembly of bacterial virulence factors. ..
  7. Natividad Ruiz; Fiscal Year: 2016
    ..In addition, understanding MurJ function will advance knowledge of the MOP superfamily of exporters, which includes members that are involved in bacterial pathogenesis and certain types of human congenital disorders of glycosylation. ..
  8. David S Cafiso; Fiscal Year: 2016
    ..Knowledge of these transport mechanisms will assist with the development of new antibiotics that can inhibit bacterial growth. ..
  9. JAMIE KATHLEEN SCOTT; Fiscal Year: 2015
    ..In addition, antigenicity, immunogenicity and structural data will be used to develop a molecular model of the MPER NCS, which should support future vaccine design. ..
  10. PRASADARAO V NEMANI; Fiscal Year: 2014
    ..The information from these studies will have a great impact on our understanding of the pathogenesis of E. coli meningitis and will also be useful for developing therapeutic approaches to treat and prevent meningitis. ..
  11. Stephen H White; Fiscal Year: 2016
    ..Our goal is to tighten the connection between the physical and biological principles as a basis for prediction algorithms. ..
  12. Inhibitors of the Y. pestis cell surface plasminogen activator for biodefense
    Marjorie H Barnes; Fiscal Year: 2010
    ..These new drugs will not be susceptible to existing resistance mechanisms and will provide a proof of principle for the efficacy of this therapeutic approach. ..
  13. Novel Erythroid Cell Membrane Protein
    Emery H Bresnick; Fiscal Year: 2010
    ....
  14. Sean X Sun; Fiscal Year: 2016
    ..In addition to potential benefits to the hearing impaired, elucidation of the physical principles underlying the outer hair cell motor will contribute to the emerging field of biological nanotechnology. ..
  15. TRACY RUTH DANIELS-WELLS; Fiscal Year: 2014
    ..Given the high expression of EMP2 on a number of malignancies, these studies will be important to establish the anti-EMP2 IgE as a potential therapeutic for cancers in women. ..
  16. Alcohol increases tight junction permeability &disrupts translocation of ZO-1
    Samantha Simet; Fiscal Year: 2012
    ..claudin-1 claudin-1 ) ..
  17. Wolfgang Baehr; Fiscal Year: 2016
    ..Transport of membrane proteins, particularly post-Golgi transport and intraflagellar transport through the cilium, are complex and insufficiently understood in disease etiology. ..
  18. Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin
    Xue Long Sun; Fiscal Year: 2013
    ....
  19. A NEW STRATEGY FOR PROTECTION FROM CEREBRAL ISCHEMIA
    Ying Xia; Fiscal Year: 2013
    ..Our preliminary studies have shown that this proposal is feasible and will very likely yield important information on the EA-induced protection and eventually shed light on new therapeutic modalities of stroke. ..
  20. The Role of the Actin Cytoskeleton and Cholesterol in Influenza Virus Assembly
    Samuel T Hess; Fiscal Year: 2010
    ..Drugs identified to have effects on viral protein clustering could be used as anti- viral therapies to reduce the spread of new strains of influenza and other viruses. ..
  21. Hemolytic Anemia: Biochemical, Molecular and Proteomic Diagnostics
    Jeffrey S Friedman; Fiscal Year: 2010
    ..We are pursuing these studies to try to find the cause of this disorder for the large group of patients for whom we can currently provide no specific diagnosis. ..
  22. Francisella tularensis Pathogenesis
    Thomas H Kawula; Fiscal Year: 2010
    ..tularensis virulence and pathogenesis. ..
  23. FLVCR protein trafficking and the regulation of heme export
    JOHN GERARD QUIGLEY; Fiscal Year: 2010
    ..and malaria, or by cellular iron overload... ..
  24. Transmembrane Regulation of Ectodomain Shedding
    Renhao Li; Fiscal Year: 2012
    ..abstract_text> ..
  25. Anti-inflammatory, cholesterol export, and cardioprotective functions of ABCA1
    Renee C LeBoeuf; Fiscal Year: 2013
    ..This information will define possible sites of impairment of these pathways that may be clinically relevant and uncover potential targets for therapeutic interventions for preventing CVD. ..
  26. David M Miller; Fiscal Year: 2016
    ..This work in C. elegans is expected to identify key determinants that also specify dendritic architecture in the human nervous system. ..
  27. John M Leong; Fiscal Year: 2014
    ....
  28. A Novel Approach to Identify HIV Suppression Factor from CD8 Cells
    Phalguni Gupta; Fiscal Year: 2010
    ..The objective of the proposed study is to use a combined biochemical and proteomic approach to conclusively identify the HIV-1 suppressive factor from the exosome-derived soluble factor. ..
  29. Michael H B Stowell; Fiscal Year: 2014
    ..abstract_text> ..
  30. Trafficking of HCN1 ion channels
    Rebecca A Piskorowski; Fiscal Year: 2010
    ..Such results may aid in the development of new disease treatments. ..
  31. Membrane biophysics of enterotoxin mediated immunomodulation
    Arnd Pralle; Fiscal Year: 2013
    ..In addition, our results will expand the current understanding of the regulation of membrane structures such as lipid domains and the membrane cytoskeleton, and will elucidate their roles in natural immune function. ..
  32. Scan of Protein Space for Optical Voltage Probes
    Lawrence B Cohen; Fiscal Year: 2011
    ..We will express the most promising FP-voltage sensors in brain slices and in vivo using viral infection followed by the production of transgenic mice. ..
  33. Characterization of a novel invasion-related gene in Glioblastoma
    David C Adamson; Fiscal Year: 2013
    ..We believe that the results of these studies will facilitate the development of molecular-based therapies that target mechanisms of tumor cell migration in GBM and could provide novel adjunctive therapeutic modalities to surgery. ..
  34. Thomas H Kawula; Fiscal Year: 2016
    ....
  35. High Throughput, Label-Fee Molecular Interaction Platform for Membrane Protein Ta
    SCOT RANDY WEINBERGER; Fiscal Year: 2010
    ....
  36. JOSEPH W ST. GEME; Fiscal Year: 2014
    ..influenzae disease. More generally, these studies will provide fundamental insights into host-microbial relationships, bacterial protein secretion, and prokaryotic protein glycosylation. ..
  37. Analysis of the interplay between cell adhesion and bacterial protein translocati
    Ralph R Isberg; Fiscal Year: 2013
    ..The ability to disrupt collaborative bacterial virulence factor interactions that promote the disease process is critical for identifying strategies that maintain immune function in the presence of pathogen attack. ..
  38. Petra Fromme; Fiscal Year: 2014
    ..The MPID center will also collaborate with the community and the PSI on the structure determination of target membrane proteins that complement the initial targets of the center. ..
  39. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..Some people require surgery or blood transfusions to treat the anemia. This application studies the what, why, and how of abnormalities of the lining of the red blood cell that cause the anemia. ..
  40. THE OUTER MEMBRANE AS A VIRULENCE PLATFORM
    DAVID ALAN HUNSTAD; Fiscal Year: 2013
    ..Understanding the functions of OmpA will lead to new strategies for preventing and treating bacterial infections. ..
  41. Arbitrary Pulse Shaping to Advance Electron Paramagnetic Resonance Tools for Biom
    SongI Han; Fiscal Year: 2011
    ....
  42. Brian M Stoltz; Fiscal Year: 2015
    ..coli induced meningitis in newborn rats. Translational medicine is the outcome of this application in which studies of basic biology and applied technology to develop new strategies of prevention will be integrated. ..
  43. Jie Liang; Fiscal Year: 2016
    ..Extensive mutant studies will be carried out to test whether designed ?-barrel membrane proteins have the intended changes in stability, in oligomerization state, as well as in geometry. ..
  44. Yinan Wei; Fiscal Year: 2014
    ..We expect the proposed research will lead to new compound classes and validate novel target mechanisms for the development of effective antimicrobial treatments for drug-resistant E. coli and other Gram-negative bacterial pathogens. ..
  45. The interaction between outer membrane porins and toll-like receptors
    T M Iverson; Fiscal Year: 2011
    ..We use a hybrid of electron microscopy, NMR, and crystallography to investigate this recognition complex, which spans two membranes in vivo. ..
  46. William Dowhan; Fiscal Year: 2015
    ..abstract_text> ..
  47. Robert G Kranz; Fiscal Year: 2016
    ..Results here will unravel the mechanisms underlying heme transport, heme red-ox control, and heme attachment to all prokaryotic and plant c-type cytochromes. ..
  48. Structural Dynamics of Drug/Proton Antiport by a Multidrug Efflux Pump
    PHILLIP RYAN STEED; Fiscal Year: 2013
    ..Additionally, these experiments will develop novel methodology to monitor the structural dynamics of a membrane protein under the influence of a stable proton gradient. ..
  49. Structure-Activity Relationship in Host Defense Peptides
    GREGORY ALEXANDER CAPUTO; Fiscal Year: 2010
    ....
  50. Structural and Functional Analyses of Toxin-Antitoxin Protein Complexes From Bact
    Celia Goulding; Fiscal Year: 2013
    ....
  51. Celia Goulding; Fiscal Year: 2016
    ..This proposal represents a unique opportunity to elucidate how specific binding is maintained as toxin-immunity pairs diverge through evolution. ..
  52. Soluble Membrane Protein Libraries: Design and use in Alzheimers drug discovery
    KYLE CHRISTOPHER WILCOX; Fiscal Year: 2012
    ....
  53. MATTHEW PHILLIP TORRES; Fiscal Year: 2015
    ..Importantly, many strong parallels exist between the yeast system being studied here and humans. Therefore, the results if these studies will have a broad impact on human G protein research. ..
  54. Console for an 800/89 DNP Spectrometer
    ROBERT GUY GRIFFIN; Fiscal Year: 2013
    ..It will therefore fill an important void in structural biology. ..
  55. Membrane Protein Expression Center
    Robert M Stroud; Fiscal Year: 2013
    ..Functional assays will serve to assist in discovery of therapeutics and understanding function. ..
  56. MEMBRANE PROTEIN STRUCTURE
    JAMES STAROS; Fiscal Year: 1990
    ..The final morning of the conference will be devoted to in-depth reviews of several membrane proteins which have been studied by a variety of structural methods...
  57. 800 MHz Low Temperature Magic Angle Spinning NMR Probe.
    Robert Griffin; Fiscal Year: 2009
    ..In addition, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available in North America and will therefore fill an important void in structural biology. ..
  58. Structural Dynamics of Cardiac Calcium Transport Regulation
    NAA ADJELEY DROMOH ABLORH; Fiscal Year: 2013
    ..The findings of this research will provide the kind of detailed molecular information that is needed for the rational design of drugs, gene therapies, and other means of treating the failing heart. ..
  59. NMR STUDIES OF MECHANISMS OF GENERAL ANESTHESIA
    Yan Xu; Fiscal Year: 2013
    ..These new studies will eventually relate the structurally specific molecular events to the functional consequences of the action ofseneral anesthetics. ..
  60. Membrane Protein Structure by Electron Crystallography
    David Stokes; Fiscal Year: 2009
    ....
  61. WILLIAM TOBEY WICKNER; Fiscal Year: 2015
    ..Human HOPS has recently been shown to have a unique and central role in Marburg and Ebola virus invasion of human cells. The proposed studies are thus important for both basic and clinical science. ..
  62. UV, CD, and NMR studies of Amphotericin B in nanoscale discoidal lipid bilayers
    THOMAS MICHAEL ANDERSON; Fiscal Year: 2013
    ..Results of these experiments will enable preparation of amphotericin B derivatives that are more effective and/or less toxic. ..
  63. Development of Bioactive Chemical Probes for Calcium-activated Chloride Channel
    Min Li; Fiscal Year: 2012
    ..The objective of this proposal is to find potent and specific small molecule chemical probes for TMEM16A that encodes the calcium-activated chloride channel (CaCC)...
  64. Role for Na,K-ATPase in Nucleoplasmic Calcium Homeostasis
    Craig Gatto; Fiscal Year: 2010
    ....
  65. REGULATION OF PULMONARY CIRCULATION IN FETUS AND NEWBORN
    Girija G Konduri; Fiscal Year: 2013
    ..These studies will identify an important new source of oxidative stress in PPHN. These observation may lead to new targeted therapies to improve vasodilation and oxygenation in PPHN. ..
  66. Molecular mechanisms of bacterial homologs of neurotransmitter:sodium symporters
    Jonathan A Javitch; Fiscal Year: 2013
    ..abstract_text> ..
  67. Mario Strazzabosco; Fiscal Year: 2016
    ....
  68. William A Cramer; Fiscal Year: 2016
    ..Via the membrane, the set of energy-transducing proteins determines the level of energy and its regulation in the human cell. ..
  69. NANCIE MACIVER; Fiscal Year: 2014
    ..The hormone leptin, which is secreted by fat cells in proportion to fat mass and affects immune function, may be responsible for linking nutritional status with immune cell function. ..
  70. Development of a novel method for inhibiting diabetic retinopathy
    David R Clemmons; Fiscal Year: 2012
    ..The work in this proposal will determine if this approach is feasible using animal models of this disease state. ..
  71. ALEXANDER SOBOLEVSKY; Fiscal Year: 2016
    ..abstract_text> ..
  72. José Antonio Lasalde-Dominicci; Fiscal Year: 2016
    ..Thus, the proposed research is relevant to NIH's mission in that it will increase current knowledge about an important ligand-gated ion channel which could help extend healthy life and reduce the burdens of illness. ..
  73. Molecular Biology of Erythrocyte Ankyrin
    Patrick G Gallagher; Fiscal Year: 2010
    ..These studies may provide important information to be used for diagnosis and treatment of patients with this disorder. ..
  74. Developing Immunotherapeutics for Methamphetamine Abuse
    THOMAS RICHARD KOSTEN; Fiscal Year: 2010
    ..Orson and Kinsey have been working on the development of DMA vaccines. Our group's combined experience is ideally positioned to get a vaccine quickly into phase 1 human studies. ..
  75. Developmental Regulation of Brain and Behavior
    JULI S WADE; Fiscal Year: 2011
    ....
  76. Genetically testing interactions of ER and oxidative stresses in retinal disease
    Douglas Gould; Fiscal Year: 2013
    ..Knowledge of the relative roles of ER and oxidative stress can be exploited in development of targeted therapeutics that could prevent or reduce vision loss in millions of people worldwide. ..
  77. FTIR STUDY OF SIGNAL TRANSDUCTION IN SENSORY RHODOPSINS
    Kenneth J Rothschild; Fiscal Year: 2010
    ..In this project we will use an array of advanced IR-based techniques to examine the detailed molecular events which lead to signal activation in SRs including new methods to study these proteins inside living cells. ..
  78. Melanopsin Signal Transduction Studied by FTIR Spectroscopy
    Kenneth J Rothschild; Fiscal Year: 2012
    ....
  79. A Molecular targeting agent for systemic radiotherapy of metastatic Prostate Canc
    Kevin P Maresca; Fiscal Year: 2010
    ..This proposal is designed to generate the pre-clinical efficacy data for the corresponding therapeutic I-131-MIP-1072 in order to assess the possibilities of developing the imaging agent into an effective treatment for prostate cancer. ..
  80. Vaccines for Sustainable Therapy of Opiate Addiction
    Frank M Orson; Fiscal Year: 2012
    ....
  81. STEVE MYER TAYLOR; Fiscal Year: 2016
    ..abstract_text> ..
  82. Function of mRNA Editing in Trypanosomes
    Stephen L Hajduk; Fiscal Year: 2010
    ..Together these studies will provide the first analysis of the role of RNA editing in protein diversification in trypanosomes and is likely to lead to the discovery of novel mitochondrial proteins. ..
  83. DC-SIGN Inhibitors for the Treatment of HIV Infection
    ALLEN BERNARD REITZ; Fiscal Year: 2013
    ....
  84. Anachem Lipidic Cubic Phase Crystallization Robot
    Robert M Stroud; Fiscal Year: 2010
    ..This proposal seeks to advance structural determination of membrane proteins in general, and targets human or pathogenic membrane proteins of importance to human health. ..
  85. Innovative Methods for Membrane Protein Crystallization
    PAUL KENIS; Fiscal Year: 2006
    ..We also intend to extend our recently developed population-balance based model capable of predicting kinetic parameters nucleation and growth of soluble proteins. ..
  86. Biomimetic Reagents that Promote Membrane Protein Stability (RMI)
    PHILIP LAIBLE; Fiscal Year: 2009
    ..The insights gained from early successes and failures should allow an intelligent and effective approach to a more demanding, intricate, and functionally diverse set of membrane proteins and membrane protein complexes. ..
  87. Molecular basis of congenital disorders of glycosylation: mechanisms of oligosach
    Smita Mohanty; Fiscal Year: 2010
    ..Thus understanding the molecular basis of function of this complex integral membrane protein is very critical for the treatment or for therapeutic intervention of the above diseases. ..
  88. Improving Rate/Quality Limitations in Membrane Protein Structure Determination
    William A Cramer; Fiscal Year: 2010
    ..The atomic structures of membrane proteins is crucial for an understanding of the molecular basis of human diseases and the development of drugs to combat them or ameliorate their consequences. ..
  89. CRYSTALLIZATION OF DIACYLGLYCEROL KINASE
    JAMES BOWIE; Fiscal Year: 2000
    ..1) Add antibody Fv fragments, specific for folded DGK. This strategy was already used to crystallize another membrane protein. (2) Add a ribonuclease S domain via the addition of S- peptide to DGK. ..
  90. Proton Crystallography of Membrane Proteins Using High Frequency ENDOR
    GARY GERFEN; Fiscal Year: 2009
    ..These specific aims bring together a number of advanced techniques in order to advance the state of the art in membrane protein structure determination. ..
  91. Innovative Methods for Membrane Protein Crystallization
    Lawrence Delucas; Fiscal Year: 2009
    ....
  92. MEMBRANE PROTEIN CRYSTALLIZATION WORKSHOP
    Janusz Sowadski; Fiscal Year: 1993
    ..Crystallization of membrane proteins for three-dimensional X-ray crystallography. Two-dimensional crystallization of membrane proteins...
  93. Enabling Nanoliter Membrane Protein Crystallization
    Rustem Ismagilov; Fiscal Year: 2009
    ..abstract_text> ..
  94. YEAST LATE GOLGI MEMBRANE PROTEIN RETENTION
    Steven Nothwehr; Fiscal Year: 1999
    ..The results of these studies should help illuminate the mechanism of yeast Golgi membrane protein retention and add significantly our overall knowledge of membrane protein sorting. ..
  95. A cholesterol biosynthetic yeast for mammalian membrane protein production
    NIALL FRASER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  96. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..1 Description, ..
  97. TRANSPORT OF AN E COLI VIRULENCE FACTOR--THE K1 CAPSULE
    Richard Silver; Fiscal Year: 2000
    ....