gag

Summary

Gene Symbol: gag
Description: Pr55(Gag)
Species: Human immunodeficiency virus 1

Top Publications

  1. pmc The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein
    Nanette Van Damme
    The San Diego Department of Veterans Affairs Healthcare System, San Diego, CA 92161, USA
    Cell Host Microbe 3:245-52. 2008
  2. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
  3. pmc Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
  4. pmc The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase
    Lavanya Krishnan
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    J Virol 84:397-406. 2010
  5. ncbi Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
    J Luban
    Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
    Cell 73:1067-78. 1993
  6. ncbi Specific incorporation of cyclophilin A into HIV-1 virions
    E K Franke
    Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
    Nature 372:359-62. 1994
  7. pmc Cytidine deaminases APOBEC3G and APOBEC3F interact with human immunodeficiency virus type 1 integrase and inhibit proviral DNA formation
    Kun Luo
    Department of Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:7238-48. 2007
  8. pmc Broad-spectrum inhibition of retroviral and filoviral particle release by tetherin
    Nolwenn Jouvenet
    Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York, USA
    J Virol 83:1837-44. 2009
  9. pmc The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 450 Brookline Avenue, CLS 1010, Boston, MA 02215, USA
    J Virol 85:7818-27. 2011
  10. pmc Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release
    H G Gottlinger
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 88:3195-9. 1991

Research Grants

Detail Information

Publications210 found, 100 shown here

  1. pmc The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein
    Nanette Van Damme
    The San Diego Department of Veterans Affairs Healthcare System, San Diego, CA 92161, USA
    Cell Host Microbe 3:245-52. 2008
    ..Endogenous BST-2 colocalizes with the HIV-1 structural protein Gag in endosomes and at the plasma membrane, suggesting that BST-2 traps virions within and on infected cells...
  2. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
    ..Our studies identify TRIM5alpha as a species-specific mediator of innate cellular resistance to HIV-1 and reveal host cell components that modulate the uncoating of a retroviral capsid...
  3. pmc Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
    ..These findings reveal a remarkable flexibility in HIV-1 nuclear transport and highlight a single residue in CA as essential in regulating interactions with NUPs...
  4. pmc The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase
    Lavanya Krishnan
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    J Virol 84:397-406. 2010
    ..We therefore conclude that capsid, not integrase, is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection...
  5. ncbi Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
    J Luban
    Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
    Cell 73:1067-78. 1993
    Retroviral Gag protein is capable of directing the assembly of virion particles independent of other retroviral elements and plays an important role early in the infection of a cell...
  6. ncbi Specific incorporation of cyclophilin A into HIV-1 virions
    E K Franke
    Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
    Nature 372:359-62. 1994
    ..We have previously shown that the principal structural protein of the human immunodeficiency virus HIV-1, the Gag polyprotein, binds the cyclophilin peptidyl-prolyl isomerases; cyclophilins catalyse a rate-limiting step in ..
  7. pmc Cytidine deaminases APOBEC3G and APOBEC3F interact with human immunodeficiency virus type 1 integrase and inhibit proviral DNA formation
    Kun Luo
    Department of Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:7238-48. 2007
    ..Thus, multiple steps of the HIV-1 replication cycle, most noticeably the formation of proviral DNA, are inhibited by both cytidine deamination-dependent and -independent mechanisms...
  8. pmc Broad-spectrum inhibition of retroviral and filoviral particle release by tetherin
    Nolwenn Jouvenet
    Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York, USA
    J Virol 83:1837-44. 2009
    ..Overall, tetherin is potentially active against many enveloped viruses and likely to be an important component of the antiviral innate immune defense...
  9. pmc The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 450 Brookline Avenue, CLS 1010, Boston, MA 02215, USA
    J Virol 85:7818-27. 2011
    ..These results suggest that capsid, likely by the qualities of its uncoating, determines whether HIV-1 requires cellular NUP153 for PIC nuclear import...
  10. pmc Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release
    H G Gottlinger
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 88:3195-9. 1991
    ..Inhibition of the release of the viral capsid proteins by interferon alpha indicates that this step of virus maturation may be sensitive to inhibition by antiviral drugs...
  11. pmc Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center and Rockefeller University, New York, New York 10021, USA
    J Virol 79:176-83. 2005
    ..Although CypA is incorporated into HIV-1 virions by virtue of CypA-Gag interactions that occur during virion assembly, in this study we show that the CypA-CA interaction that occurs ..
  12. pmc HIV-1 Gag extension: conformational changes require simultaneous interaction with membrane and nucleic acid
    Siddhartha A K Datta
    HIV Drug Resistance Program, National Cancer Institute, PO Box B, Building 535, Fredrick, MD 21702 1201, USA
    J Mol Biol 406:205-14. 2011
    The retroviral Gag polyprotein mediates viral assembly. The Gag protein has been shown to interact with other Gag proteins, with the viral RNA, and with the cell membrane during the assembly process...
  13. pmc The role of cellular factors in promoting HIV budding
    Eric R Weiss
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Mol Biol 410:525-33. 2011
    ..HIV-1 Gag directly interacts with early-acting components of this pathway, which ultimately triggers the assembly of the ..
  14. pmc Gag cytotoxic T lymphocyte escape mutations can increase sensitivity of HIV-1 to human TRIM5alpha, linking intrinsic and acquired immunity
    Emilie Battivelli
    INSERM U941, Hopital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Virol 85:11846-54. 2011
    ..Taken together, these findings suggest that CTL mutations may influence HIV-1 replication by modifying both viral infectivity and sensitivity to TRIM5α...
  15. ncbi AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly
    Xinhong Dong
    Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Cell 120:663-74. 2005
    b>Gag proteins direct the process of retroviral particle assembly and form the major protein constituents of the viral core...
  16. pmc Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane binding
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Room 5736A, Ann Arbor, MI 48109, USA
    J Virol 82:2405-17. 2008
    Human immunodeficiency virus type 1 (HIV-1) particle assembly mediated by the viral structural protein Gag occurs predominantly on the plasma membrane (PM)...
  17. pmc Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments
    Yuyang Tang
    Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, TN 37208, USA
    J Virol 83:7982-95. 2009
    ..Examination of these cells using confocal microscopy showed an accumulation and stabilization of Gag in LE/L compartments...
  18. doi Anti-retroviral activity of TRIM5 alpha
    Emi E Nakayama
    Department of Viral Infections, Research Institute for Microbial Disease, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Rev Med Virol 20:77-92. 2010
    ..Accurate structural analysis of the binding surface between the viral capsid protein and TRIM5 alpha SPRY is thus required for the development of new antiretroviral drugs that enhance anti-HIV-1 activity of human TRIM5 alpha...
  19. pmc Moloney leukemia virus 10 (MOV10) protein inhibits retrovirus replication
    Xiaojun Wang
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 285:14346-55. 2010
    ..All of these results indicate that MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection...
  20. pmc Small-molecule inhibition of human immunodeficiency virus type 1 infection by virus capsid destabilization
    Jiong Shi
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 85:542-9. 2011
    ..This study highlights uncoating as a step in the HIV-1 life cycle that is susceptible to small molecule intervention...
  21. pmc Revisiting HIV-1 uncoating
    Nathalie Arhel
    Department of Virology, URA3015, Institut Pasteur, 25 28 rue du Dr, Roux, 75015 Paris, France
    Retrovirology 7:96. 2010
    ....
  22. pmc Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function
    Anna Kula
    Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology ICGEB, Padriciano, 99, 34012 Trieste, Italy
    Retrovirology 8:60. 2011
    ....
  23. pmc A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication
    Man Lung Yeung
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 284:19463-73. 2009
    ..We discuss the similarities and differences between our shRNA findings for HIV-1 using a spreading infection assay in human Jurkat T-cells and results from other investigators who used siRNA-based screenings in HeLa or 293T cells...
  24. pmc Molecular determinants that regulate plasma membrane association of HIV-1 Gag
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Mol Biol 410:512-24. 2011
    ..Targeting and binding of Gag to the PM are the first steps in this assembly process and are mediated by the matrix domain of Gag...
  25. pmc HIV-1 capsid-targeting domain of cleavage and polyadenylation specificity factor 6
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
    J Virol 86:3851-60. 2012
    ..Given the sensitivity of different primate lentiviruses to CPSF6-358 and apparent conservation of this interaction, our data suggest that CPSF6-358-mediated targeting of HIV-1 could provide a broadly effective antiviral strategy...
  26. pmc CPSF6 defines a conserved capsid interface that modulates HIV-1 replication
    Amanda J Price
    Medical Research Council Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Cambridge, United Kingdom
    PLoS Pathog 8:e1002896. 2012
    ..Furthermore, mutations that prevent CPSF6 binding also relieve dependence on nuclear entry cofactors TNPO3 and RanBP2. These results suggest that the HIV-1 capsid mediates direct host cofactor interactions to facilitate viral infection...
  27. ncbi Structure of the amino-terminal core domain of the HIV-1 capsid protein
    R K Gitti
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21228, USA
    Science 273:231-5. 1996
    ..In the free monomeric domain, Pro90 adopts kinetically trapped cis and trans conformations, raising the possibility that cyclophilin A catalyzes interconversion of the cis- and trans-Pro90 loop structures...
  28. pmc Target cell type-dependent modulation of human immunodeficiency virus type 1 capsid disassembly by cyclophilin A
    Yuan Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 83:10951-62. 2009
    ..In both cell types examined, decreased stability of the capsid was associated with a decrease in the efficiency of HIV-1 infection...
  29. pmc Dynamics of ESCRT protein recruitment during retroviral assembly
    Nolwenn Jouvenet
    Aaron Diamond AIDS Research Center, Laboratory of Retrovirology, The Rockefeller University, New York, 10065 New York, USA
    Nat Cell Biol 13:394-401. 2011
    ..accumulated at viral assembly sites, coincident with the accumulation of the main viral structural protein, Gag, and was not recycled after assembly...
  30. pmc Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1002009. 2011
    ....
  31. pmc Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses
    D Braaten
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 70:4220-7. 1996
    The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein binds to cyclophilin A and incorporates this cellular peptidyl prolyl-isomerase into virions...
  32. pmc Binding of the human immunodeficiency virus type 1 Gag polyprotein to cyclophilin A is mediated by the central region of capsid and requires Gag dimerization
    J Colgan
    Department of Microbiology, College of Physicians and Surgeons of Columbia University, New York, New York 10032USA
    J Virol 70:4299-310. 1996
    ..CyPA) is incorporated into human immunodeficiency virus type 1 (HIV-1) virions via direct contacts with the HIV-1 Gag polyprotein...
  33. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..The UEV domain of Tsg101 binds to an essential tetrapeptide (PTAP) motif within the p6 domain of the structural Gag protein and also to ubiquitin...
  34. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..The primary late assembly (L) domain in the p6 region of HIV-1 Gag mediates the detachment of the virion by recruiting host Tsg101, a component of the class E vacuolar protein ..
  35. doi Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein
    Vanda Bartonova
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:32024-33. 2008
    Morphogenesis of infectious HIV-1 involves budding of immature virions followed by proteolytic disassembly of the Gag protein shell and subsequent assembly of processed capsid proteins (CA) into the mature HIV-1 core...
  36. doi Site-specific characterization of HIV-1 nucleocapsid protein binding to oligonucleotides with two binding sites
    Sergiy V Avilov
    Laboratoire Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    Biochemistry 48:2422-30. 2009
    ..Moreover, our data suggest that binding of NC even to close binding sites shows no strong cooperativity...
  37. pmc Matrix mediates the functional link between human immunodeficiency virus type 1 RNA nuclear export elements and the assembly competency of Gag in murine cells
    Nathan M Sherer
    Department of Infectious Diseases, King s College School of Medicine, London SE1 9RT, United Kingdom
    J Virol 83:8525-35. 2009
    ..Human immunodeficiency virus type 1 (HIV-1) assembles poorly in murine cells, reflecting inefficient targeting of the Gag structural polyprotein to the plasma membrane...
  38. pmc Cellular microRNA and P bodies modulate host-HIV-1 interactions
    Robin Nathans
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 34:696-709. 2009
    ..Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses...
  39. ncbi Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2
    Melissa Batonick
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Virology 342:190-200. 2005
    ..its synthesis, Env, therefore, is retrieved from the cellular surface unless internalization is inhibited by viral Gag. Here we demonstrate that not only Env, but also HIV-1 Gag, specifically binds to AP-2...
  40. pmc The structural biology of HIV assembly
    Barbie K Ganser-Pornillos
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 18:203-17. 2008
    ..through the formation of morphologically distinct immature and mature viral capsids that are organized by the Gag polyprotein (immature) and by the fully processed CA protein (mature)...
  41. pmc Dynamics of HIV-1 assembly and release
    Sergey Ivanchenko
    Physical Chemistry, Department of Chemistry and Biochemistry, Munich Center for Integrated Protein Science CiPSM and Center for NanoScience, Ludwig Maximilians Universitat Munchen, Munich, Germany
    PLoS Pathog 5:e1000652. 2009
    ..release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein...
  42. ncbi Beyond Tsg101: the role of Alix in 'ESCRTing' HIV-1
    Ken Fujii
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland 21702 1201, USA
    Nat Rev Microbiol 5:912-6. 2007
    ..Here, we focus on the use of host-cell factors during HIV-1 budding and highlight recent progress in our understanding of the role of one such factor, Alix, in both viral and cellular membrane budding and fission events...
  43. pmc Biochemical and biophysical characterization of a chimeric TRIM21-TRIM5alpha protein
    Alak Kanti Kar
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 82:11669-81. 2008
    ..2(SPRY) domain decreased capsid binding. Thus, the TRIM5(Rh)-21R restriction factor can directly recognize retroviral capsid-like complexes in the absence of other mammalian proteins...
  44. pmc Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment
    Anjali Joshi
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland 21702 1201, USA
    J Virol 83:5375-87. 2009
    ..In the current study, we make use of an HIV-1 Gag-matrix mutant, 29/31KE, that is MVB targeted...
  45. pmc Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 107:14787-92. 2010
    ..TMG capping of unspliced/partially spliced HIV-1 RNAs represents a new regulatory mechanism for selective expression...
  46. pmc Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    Retrovirology 8:61. 2011
    ..This binding interaction stabilizes unspliced and partially spliced HIV-1 transcripts leading to increased cytoplasmic expression of these viral RNAs...
  47. pmc Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15
    Atsushi Okumura
    The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:1440-5. 2006
    ..ISG15 expression specifically inhibited ubiquitination of Gag and Tsg101 and disrupted the interaction of the Gag L domain with Tsg101, but conjugation of ISG15 to Gag or Tsg101 ..
  48. pmc Cyclosporine A-resistant human immunodeficiency virus type 1 mutants demonstrate that Gag encodes the functional target of cyclophilin A
    D Braaten
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York 10032, USA
    J Virol 70:5170-6. 1996
    ..A is incorporated into human immunodeficiency virus type 1 virions via contacts with the proline-rich domain of the Gag polyprotein...
  49. pmc Transfer of the HIV-1 cyclophilin-binding site to simian immunodeficiency virus from Macaca mulatta can confer both cyclosporin sensitivity and cyclosporin dependence
    A A Bukovsky
    Division of Human Retrovirology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:10943-8. 1997
    ..is mediated by a specific interaction between the active site of the enzyme and the capsid (CA) domain of the HIV-1 Gag polyprotein...
  50. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
    ..AIP1 also binds the HIV-1 p6(Gag) and EIAV p9(Gag) proteins, indicating that it can function directly in virus budding...
  51. pmc Unexpected roles for UPF1 in HIV-1 RNA metabolism and translation
    Lara Ajamian
    HIV 1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    RNA 14:914-27. 2008
    The HIV-1 ribonucleoprotein (RNP) contains the major structural protein, pr55(Gag), viral genomic RNA, as well as the host protein, Staufen1...
  52. pmc Basic residues in the nucleocapsid domain of Gag are critical for late events of HIV-1 budding
    Vincent Dussupt
    Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, MD 20892, USA
    J Virol 85:2304-15. 2011
    The p6 region of HIV-1 Gag contains two late (L) domains, PTAP and LYPXnL, that bind the cellular proteins Tsg101 and Alix, respectively...
  53. pmc Filamin A protein interacts with human immunodeficiency virus type 1 Gag protein and contributes to productive particle assembly
    Joann Cooper
    Center for AIDS Health Disparities Research, Department of Microbiology, and Immunology, Meharry Medical College, Nashville, Tennessee 37208, USA
    J Biol Chem 286:28498-510. 2011
    HIV-1 Gag precursor directs virus particle assembly and release...
  54. pmc HIV-1 matrix protein binding to RNA
    Ayna Alfadhli
    Vollum Institute and Department of Microbiology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    J Mol Biol 410:653-66. 2011
    The matrix (MA) domain of the human immunodeficiency virus type 1 (HIV-1) precursor Gag (PrGag) protein plays multiple roles in the viral replication cycle...
  55. pmc Second-site suppressors of HIV-1 capsid mutations: restoration of intracellular activities without correction of intrinsic capsid stability defects
    Ruifeng Yang
    Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Retrovirology 9:30. 2012
    ..Based on previous studies of HIV-1 CA mutants exhibiting altered capsid stability, we concluded that formation of a capsid of optimal intrinsic stability is crucial for HIV-1 infection...
  56. pmc Defective HIV-1 particle assembly in AP-3-deficient cells derived from patients with Hermansky-Pudlak syndrome type 2
    Ling Liu
    Department of Microbiology and Immunology and Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, Tennessee, USA
    J Virol 86:11242-53. 2012
    ..In contrast, virus-like particle release mediated by MA-deficient Gag mutants lacking the AP-3 binding site was not altered in HPS2 cells, indicating that the MA domain serves as the ..
  57. pmc Analysis of human immunodeficiency virus type 1 Gag ubiquitination
    Eva Gottwein
    Abteilung Virologie, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Virol 79:9134-44. 2005
    ..of human immunodeficiency virus type 1 (HIV-1) and several other retroviruses, but the functional significance of Gag ubiquitination is unknown. To address this problem, we decided to analyze Gag ubiquitination in detail...
  58. pmc Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity
    Hans Rempel
    Department of Laboratory Medicine at the Veterans Affairs Medical Center, San Francisco, California, United States of America
    PLoS ONE 3:e1967. 2008
    ....
  59. pmc HIV-1 nucleocapsid protein switches the pathway of transactivation response element RNA/DNA annealing from loop-loop "kissing" to "zipper"
    My Nuong Vo
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 386:789-801. 2009
    ....
  60. pmc The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding
    Vincent Dussupt
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 5:e1000339. 2009
    HIV-1 release is mediated through two motifs in the p6 region of Gag, PTAP and LYPX(n)L, which recruit cellular proteins Tsg101 and Alix, respectively...
  61. pmc Entropic switch regulates myristate exposure in the HIV-1 matrix protein
    Chun Tang
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 5398, USA
    Proc Natl Acad Sci U S A 101:517-22. 2004
    ..myristoylated matrix protein (myr-MA) of HIV functions as a regulator of intracellular localization, targeting the Gag precursor polyprotein to lipid rafts in the plasma membrane during virus assembly and dissociating from the ..
  62. ncbi A single amino acid change in the SPRY domain of human Trim5alpha leads to HIV-1 restriction
    Melvyn W Yap
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Curr Biol 15:73-8. 2005
    ..A single amino acid substitution (R332P) in the human Trim5alpha can confer the ability to restrict HIV-1, suggesting that small changes during evolution may have profound effects on our susceptibility to cross-species infection...
  63. doi Solution structure of a double mutant of the carboxy-terminal dimerization domain of the HIV-1 capsid protein
    Hing C Wong
    Comprehensive Cancer Center, The University of Alabama at Birmingham, Birmingham, Alabama 35294 2041, USA
    Biochemistry 47:2289-97. 2008
    ..interface that includes the major homology region (MHR) has been suggested as the functional form during the Gag assembly...
  64. pmc The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane
    Amy B Strasner
    Intergrated Bioscience Program in Immunobiology, Huck Institute of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    J Immunol 181:3706-13. 2008
    HIV type 1 (HIV-1) assembly and egress are driven by the viral protein Gag and occur at the plasma membrane in T cells...
  65. pmc Gag mutations strongly contribute to HIV-1 resistance to protease inhibitors in highly drug-experienced patients besides compensating for fitness loss
    Elisabeth Dam
    INSERM U552, Paris, France
    PLoS Pathog 5:e1000345. 2009
    ..Additional mutations compensating for the RC loss subsequently accumulate within PR and in Gag substrate cleavage sites...
  66. pmc Dynamics and timing of in vivo mutations at Gag residue 242 during primary HIV-1 subtype C infection
    Vladimir Novitsky
    Harvard School of Public Health AIDS Initiative, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Virology 403:37-46. 2010
    Viral mutations at Gag residue 242 and relevant viral polymorphisms were analyzed in a cohort of 42 individuals with primary HIV-1 subtype C infection using single-genome amplification/sequencing...
  67. pmc Gyrase B inhibitor impairs HIV-1 replication by targeting Hsp90 and the capsid protein
    Luciano Vozzolo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, 46 Cleveland Street, W1T 4JF London, United Kingdom
    J Biol Chem 285:39314-28. 2010
    ..Both activities were amenable to inhibition by small molecules and represent novel antiretroviral drug targets...
  68. pmc Host HLA B*allele-associated multi-clade Gag T-cell recognition correlates with slow HIV-1 disease progression in antiretroviral therapy-naïve Ugandans
    Jennifer Serwanga
    MRC UVRI Uganda Research Unit on AIDS, c o Uganda Virus Research Institute, Entebbe, Uganda
    PLoS ONE 4:e4188. 2009
    ..In this study, possible interactions between viral load, CD4 T cell slopes, host genetics and HIV-specific IFN-gamma responses were evaluated in chronically HIV-1-infected adults...
  69. pmc Human immunodeficiency virus type 1 reverse transcriptase: enhancement of activity by interaction with cellular topoisomerase I
    H Takahashi
    Department of Pathology, National Institute of Health, Tokyo, Japan
    Proc Natl Acad Sci U S A 92:5694-8. 1995
    ..We have investigated a possible association of HIV-1 gag proteins with topo I activity...
  70. pmc Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import
    P Gallay
    Infectious Disease Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 70:1027-32. 1996
    ..In addition, our data suggest that Vpr governs HIV-1 nuclear import through a distinct pathway...
  71. pmc Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1
    Cagan Gurer
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 79:910-7. 2005
    The p6 domain of the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein mediates virion budding from infected cells via protein-protein contacts with the class E vacuolar protein sorting factors, Tsg101 and AIP1/ALIX...
  72. pmc Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription
    My Nuong Vo
    Department of Chemistry and Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 386:773-88. 2009
    ....
  73. ncbi Structural similarity between the p17 matrix protein of HIV-1 and interferon-gamma
    S Matthews
    Department of Biochemistry, University of Oxford, UK
    Nature 370:666-8. 1994
    ..The protein has several key functions. It orchestrates viral assembly via targeting signals that direct the gag precursor polyprotein, p55, to the host cell membrane and it interacts with the transmembrane protein, gp41, to ..
  74. ncbi Human plasmatic apolipoprotein H binds human immunodeficiency virus type 1 and type 2 proteins
    E Stefas
    OSRTOM, UR41, Montpellier, France
    AIDS Res Hum Retroviruses 13:97-104. 1997
    ..assay and ligand blot and dot assays, apo H was able to bind recombinant retroviral HIV antigens, especially Gag proteins p18 of HIV-1, p26 of HIV-2, and Env gp160 of HIV-1...
  75. pmc The Nef protein of human immunodeficiency virus type 1 enhances serine phosphorylation of the viral matrix
    S Swingler
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 71:4372-7. 1997
    ..Recombinant p21-activated kinase hPAK65, a recently proposed relative of the Nef-associated kinase, achieved a comparable result. Taken together, these data suggest that MA is a target of the Nef-associated serine kinase...
  76. ncbi HIV-1 nuclear import: in search of a leader
    M I Bukrinsky
    The Picower Institute for Medical Research, Manhasset, NY 11030, USA
    Front Biosci 2:d578-87. 1997
    ..We also describe a class of novel anti-HIV compounds which target the NLSs of HIV-1 and effectively block viral replication in T cells and macrophages...
  77. pmc HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr
    M I Bukrinsky
    Picower Institute for Medical Research, Manhasset, NY 11030, USA
    Mol Med 4:138-43. 1998
  78. pmc CNI-H0294, a nuclear importation inhibitor of the human immunodeficiency virus type 1 genome, abrogates virus replication in infected activated peripheral blood mononuclear cells
    O K Haffar
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA
    Antimicrob Agents Chemother 42:1133-8. 1998
    ..These results support nuclear importation as a novel target and CNI-H0294 and its derivatives as novel compounds for therapeutic intervention in HIV infection and AIDS...
  79. ncbi Enhancement of the basal-level activity of HIV-1 long terminal repeat by HIV-1 nucleocapsid protein
    J L Zhang
    Division of Infectious Diseases, Charles A Dana Research Institute, Boston, Massachusetts, USA
    Virology 268:251-63. 2000
    ..Transient transfection of human T lymphocytes with the plasmids containing HIV-1 nc or gag showed enhancement of LTR-CAT activity...
  80. ncbi HIV-1 matrix protein p17 enhances the proliferative activity of natural killer cells and increases their ability to secrete proinflammatory cytokines
    Marco Vitale
    Department of Anatomy, Pharmacology and Forensic Medicine, Ospedale Maggiore, University of Parma, Italy
    Br J Haematol 120:337-43. 2003
    ..The property of p17 to increase the production of TNF-alpha and IFN-gamma might be a mechanism used by HIV-1 to modulate the immune system to support its replication and spreading...
  81. ncbi Nucleolin and the packaging signal, psi, promote the budding of human immunodeficiency virus type-1 (HIV-1)
    Tomonori Ueno
    Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 48:111-8. 2004
    b>Gag proteins of human immunodeficiency virus type 1 (HIV-1) play a pivotal role in the budding of the virion, in which the zinc finger motifs of the gag proteins recognize the packaging signal of genomic RNA...
  82. ncbi The tRNALys packaging complex in HIV-1
    Lawrence Kleiman
    Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote St Catherine Road, Montreal, Que, Canada H3T 1E2
    Int J Biochem Cell Biol 36:1776-86. 2004
    ..supports a model for the formation of a tRNALys packaging complex, whose components include the precursor proteins Gag and Gag-Pol, viral genomic RNA, tRNALys, and lysyl-tRNA synthetase (LysRS)...
  83. ncbi Novel activities of cyclophilin A and cyclosporin A during HIV-1 infection of primary lymphocytes and macrophages
    Manisha Saini
    Molecular Virology Division, St Luke s Roosevelt Hospital Center, Columbia University Medical Center, 432 West 58th Street, New York, NY 10019, USA
    J Immunol 177:443-9. 2006
    ..at both early and late phases of virus replication, the stage affected is determined by the sequence of HIV-1 Gag. Because the cell type infected determines the identity of host proteins active in HIV-1 replication and can ..
  84. pmc Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants
    Patrick Kaumanns
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Retrovirology 3:79. 2006
    ..2 and 19.6 fold restriction)...
  85. pmc Influence of dominant HIV-1 epitopes on HLA-A3/peptide complex formation
    Judith Racape
    Departement d Immunologie, Institut Cochin, F 75014 Paris, France
    Proc Natl Acad Sci U S A 103:18208-13. 2006
    ..Such observations may have great implications for T cell antigen receptor recognition and the immunogenicity of HLA/peptide complexes...
  86. pmc Electrostatic interactions drive membrane association of the human immunodeficiency virus type 1 Gag MA domain
    Amanda K Dalton
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    J Virol 81:6434-45. 2007
    ..Membrane association is directed by MA, the N-terminal domain of the Gag structural protein...
  87. pmc Refined study of the interaction between HIV-1 p6 late domain and ALIX
    Carine Lazert
    Universite Lyon 1, Centre National de Recherche Scientifique CNRS, VirPatH FRE 3011, Faculté de médecine RTH Laennec, Lyon, France
    Retrovirology 5:39. 2008
    ..Altogether, our data support a model where the C-terminal proline-rich domain of ALIX allows the access of its binding site to p6 by alleviating a conformational constraint resulting from the presence of the central p6 hinge...
  88. doi Structural studies of HIV-1 Gag p6ct and its interaction with Vpr determined by solution nuclear magnetic resonance
    Gilmar F Salgado
    Unite de Pharmacologie Chimique et Genetique, INSERM U640, CNRS UMR8151, UFR des Sciences Pharmaceutiques et Biologiques, 4, avenue de l Observatoire, 75270 Paris Cedex 06, France
    Biochemistry 48:2355-67. 2009
    ..protein R (Vpr) incorporation in sorting virions relies greatly on the interaction with the group-specific antigen (Gag) C-terminal region, which encompasses protein p6...
  89. pmc The nucleocapsid region of human immunodeficiency virus type 1 Gag assists in the coordination of assembly and Gag processing: role for RNA-Gag binding in the early stages of assembly
    David E Ott
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 83:7718-27. 2009
    Human immunodeficiency virus type 1 (HIV-1) Gag-RNA interactions are required for virus assembly...
  90. pmc Analysis of the percentage of human immunodeficiency virus type 1 sequences that are hypermutated and markers of disease progression in a longitudinal cohort, including one individual with a partially defective Vif
    Anne Piantadosi
    Fred Hutchinson Cancer Center, Seattle, WA 98109 1024, USA
    J Virol 83:7805-14. 2009
    ..By examining single-copy gag sequences from proviral DNA, hypermutation was detected in 16 of 28 individuals...
  91. doi High prevalence of natural polymorphisms in Gag (CA-SP1) associated with reduced response to Bevirimat, an HIV-1 maturation inhibitor
    Eduardo Seclen
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS 24:467-9. 2010
    ..Conversely, only four patients (1%) harbored major bevirimat resistance mutations. Finally, specific gag changes were associated with protease inhibitor resistance mutations in subtype B viruses.
  92. doi Highly sensitive analysis of the interaction between HIV-1 Gag and phosphoinositide derivatives based on surface plasmon resonance
    Kensaku Anraku
    Institute of Health Sciences, Kumamoto Health Science University, 325 Izumi machi, Kumamoto 861 5598, Japan
    Biochemistry 49:5109-16. 2010
    Human immunodeficiency virus type 1 (HIV-1) Gag protein is the principal structural component of the HIV particle. Localization of the Pr55(Gag) protein to the plasma membrane initiates virus assembly...
  93. pmc Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition
    M D Miller
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 71:5382-90. 1997
    ..Only molecules correctly cleaved by integrase protein at the 3' ends were competent to integrate, suggesting that one role for terminal cleavage by integrase may be to create a defined end at otherwise heterogeneous cDNA termini...
  94. pmc Viral protein R regulates nuclear import of the HIV-1 pre-integration complex
    S Popov
    The Picower Institute for Medical Research, Manhasset, NY 11030, USA
    EMBO J 17:909-17. 1998
    ..These results identify the biochemical mechanism of Vpr function in transport of the viral pre-integration complex to, and across, the nuclear membrane...
  95. pmc Translation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein
    A Cimarelli
    Departments of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 73:5388-401. 1999
    Human immunodeficiency virus type 1 (HIV-1) gag-encoded proteins play key functions at almost all stages of the viral life cycle...
  96. pmc Species-specific variation in the B30.2(SPRY) domain of TRIM5alpha determines the potency of human immunodeficiency virus restriction
    Matthew Stremlau
    Dana Farber Cancer Institute, 44 Binney St, JFB 824, Boston, MA 02115, USA
    J Virol 79:3139-45. 2005
    ..These observations implicate the v1 variable region of the B30.2(SPRY) domain in TRIM5alpha(rh) antiviral potency...
  97. pmc Myristoylation is required for human immunodeficiency virus type 1 Gag-Gag multimerization in mammalian cells
    Hua Li
    Department of Pediatrics, Emory University, Atlanta, GA 30322, USA
    J Virol 81:12899-910. 2007
    The Gag protein of human immunodeficiency virus type 1 directs the virion assembly process. Gag proteins must extensively multimerize during the formation of the spherical immature virion shell...
  98. pmc Mutations in human immunodeficiency virus type 1 nucleocapsid protein zinc fingers cause premature reverse transcription
    James A Thomas
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, MD 21702 1201, USA
    J Virol 82:9318-28. 2008
    ..virions bud from producer cells, but we fail to see any evidence that the NC mutations alter the timing of Pr55(Gag) processing...
  99. pmc Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA
    Yeshitila N Friew
    HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    Retrovirology 6:56. 2009
    ..is a dimer and its virion incorporation is mediated through interactions with viral or nonviral RNAs and/or HIV-1 Gag. We have now employed a bimolecular fluorescence complementation assay (BiFC) to analyze the intracellular A3G-A3G, ..
  100. pmc HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity
    Barbara Muller
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Biol Chem 284:29692-703. 2009
    ..HIV) polyprotein processing, but there is no direct quantitative correlation between the degree of impairment of Gag processing and virion infectivity at low PI concentrations...
  101. pmc Myristoylation-dependent replication and assembly of human immunodeficiency virus 1
    M Bryant
    Department of Pediatrics, Washington University School of Medicine, Saint Louis, MO 63110
    Proc Natl Acad Sci U S A 87:523-7. 1990
    ..Complementation of this defect, in trans, restores infectious particle production. The nonmyristoylated (myr-) gag precursor accumulates in infected cells and is not processed into the mature capsid components of the intact virion...

Research Grants24

  1. STRUCTURE/FUNCTION OF HIV1 CAPSID/CYCLOPHILIN COMPLEX
    Wesley Sundquist; Fiscal Year: 2001
    ..Finally, genetic analysis of mutants of CA in an HIV provirus will be used to test models that arise from the CA and CA-cyclophilin structures. ..
  2. Structural Biology of HIV Assembly, Budding, and Entry
    Wesley Sundquist; Fiscal Year: 2006
    ..In summary, our Program will provide significant insights into how the HIV virion functions as a machine that can enter and exit cells, and how these processes can be inhibited. ..
  3. Role of Lysyl-tRNA Synthetase in HIV-1 Replication
    Lawrence Kleiman; Fiscal Year: 2006
    ..b>Gag alone will package LysRS into Gag particles...
  4. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    JAISRI LINGAPPA; Fiscal Year: 2008
    ..abstract_text> ..
  5. Cell-free VEE assembly system and alphavirus drug screen
    JAISRI LINGAPPA; Fiscal Year: 2006
    ..The cell-free VEE assembly system offer a safe and low cost approach to understanding the mechanism of VEE capsid formation and to screen for agents active against alphaviruses that have potential as bioterrorism agents. ..
  6. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2009
    ..by applicant): The overall goal of the research proposed in this renewal application is to understand how HIV-1 Gag is trafficked through the cell, targeted to membranes and assembled into virions...
  7. Glial cell differentiation and glioma formation
    MARILYN RESH; Fiscal Year: 2006
    ..The activity of the above proteins in human glioma tumor samples will be determined. ..
  8. Mechanisms of AID-dependent Adaptive and Innate Immunity
    Reuben S Harris; Fiscal Year: 2010
    ..abstract_text> ..
  9. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  10. Virus-Mosquito mRNA Stability
    Jeffrey Wilusz; Fiscal Year: 2009
    ..In summary, this information will provide fundamental insights into insect molecular biology and virus-host interactions that will provide the groundwork for novel avenues of arbovirus control. ..
  11. Nicotine in HIV-1 and Mycobacterium tuberculosis Neuropathogenesis
    ROBERT ROCK; Fiscal Year: 2008
    ..abstract_text> ..
  12. Incident HIV Infection and Immune Response to Hepatitis C Virus
    Andrea Cox; Fiscal Year: 2007
    ..This, along with excellent mentorship and the supportive and rich environment at JHU, will provide Dr. Cox with the skills she needs to develop into an independent researcher studying HIV/HCV coinfection immunology. ..
  13. HIV Replication and Thymopoiesis in Adolescents
    Paul Krogstad; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  14. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  15. Regulation of mRNA Decapping in Human Cells
    Jeffrey Wilusz; Fiscal Year: 2004
    ..In total, these studies should provide pivotal insights into this important area of post-transcriptional regulation of gene expression in mammalian cells. ..
  16. DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1
    Mark Wainberg; Fiscal Year: 2001
    ..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
  17. Typhoon 8600 Variable Mode Imager Workstation
    Jeffrey Wilusz; Fiscal Year: 2002
    ..abstract_text> ..
  18. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  19. Design and Testing of a Parechovirus Vaccine Vector
    Paul Krogstad; Fiscal Year: 2005
    ..We propose to construct and complete the initial in vitro testing of a parechovirus based vaccine vector, based on strategies already employed in the successful construction of enteroviral vectors. ..
  20. Cellular Immunology of Enterovirus Infection
    Paul Krogstad; Fiscal Year: 2007
    ..These studies will provide a foundation for further studies of host-pathogen relationships in picornavirus disease and the potential utility of picornaviruses as enterally delivered vaccine vectors. [unreadable] [unreadable] [unreadable]..
  21. Anti-HIV Therapeutics that Target HIV Nuclear Import
    Omar Haffar; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATION: Not Available ..