Oct4

Summary

Gene Symbol: Oct4
Description: POU class 5 homeobox 1
Alias: OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, POU domain, class 5, transcription factor 1, POU class 5 homeobox 1 transcript variant OCT4B1, POU class 5 homeobox 1 transcript variant OCT4B2, POU class 5 homeobox 1 transcript variant OCT4B3, POU class 5 homeobox 1 transcript variant OCT4B4, POU class 5 homeobox 1 transcript variant OCT4B5, POU class 5 homeobox 1 transcript variant OCT4B6, POU classV homeobox 1 variant 2, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
Species: human

Top Publications

  1. ncbi Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
    H Niwa
    Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
    Nat Genet 24:372-6. 2000
  2. doi Coexpression of Oct4 and Nanog enhances malignancy in lung adenocarcinoma by inducing cancer stem cell-like properties and epithelial-mesenchymal transdifferentiation
    Shih Hwa Chiou
    Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan
    Cancer Res 70:10433-44. 2010
  3. doi Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
    Shih Hwa Chiou
    Institutes of Oral Biology, Traditional Medicine, and Anatomy and Cell Biology, National Yang Ming University, Taiwan, People s Republic of China
    Clin Cancer Res 14:4085-95. 2008
  4. doi Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms
    David M Kristensen
    University Department of Growth and Reproduction, Section GR5064, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark
    Hum Reprod 23:775-82. 2008
  5. doi Pseudogene OCT4-pg4 functions as a natural micro RNA sponge to regulate OCT4 expression by competing for miR-145 in hepatocellular carcinoma
    Lei Wang
    State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi an, China
    Carcinogenesis 34:1773-81. 2013
  6. doi Prognostication of OCT4 isoform expression in prostate cancer
    Marina França de Resende
    Department of Anatomic Pathology, A C Camargo Cancer Hospital, Rua Professor Antonio Prudente 211, Liberdade, Sao Paulo, SP, 01509 900, Brazil
    Tumour Biol 34:2665-73. 2013
  7. doi Expression pattern of Oct-4, Sox2, and c-Myc in the primary culture of human dental pulp derived cells
    Lu Liu
    Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat Sen University, Guangzhou, Guangdong, China
    J Endod 37:466-72. 2011
  8. ncbi OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer
    Yaser Atlasi
    Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
    Int J Cancer 120:1598-602. 2007
  9. pmc Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract
    Christel T Freberg
    Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 18:1543-53. 2007
  10. doi Oct-3/4 expression reflects tumor progression and regulates motility of bladder cancer cells
    Chao Ching Chang
    Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan
    Cancer Res 68:6281-91. 2008

Research Grants

  1. TSHR and thyrocyte development
    Reigh Yi Lin; Fiscal Year: 2013
  2. Herman S Cheung; Fiscal Year: 2014
  3. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
  4. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
  5. Function of KLF4-expressing stem cells in mouse cutaneous wound healing
    Walden Ai; Fiscal Year: 2013
  6. Mana M Parast; Fiscal Year: 2014
  7. Cardiovascular and Therapeutic Potential of Reprogrammed Human Fibroblasts
    WILLIAM ROBB MACLELLAN; Fiscal Year: 2010
  8. Marian L Waterman; Fiscal Year: 2016
  9. Ming Ming Zhou; Fiscal Year: 2014
  10. Recapitulating developmental pathways to improve iPSC-RPE derivation protocols
    PETER DWAYNE WESTENSKOW; Fiscal Year: 2013

Detail Information

Publications250 found, 100 shown here

  1. ncbi Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
    H Niwa
    Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
    Nat Genet 24:372-6. 2000
    ....
  2. doi Coexpression of Oct4 and Nanog enhances malignancy in lung adenocarcinoma by inducing cancer stem cell-like properties and epithelial-mesenchymal transdifferentiation
    Shih Hwa Chiou
    Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan
    Cancer Res 70:10433-44. 2010
    ..Though Oct4 and Nanog are homebox transcription factors essential to the self-renewal of stem cells and are expressed in ..
  3. doi Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
    Shih Hwa Chiou
    Institutes of Oral Biology, Traditional Medicine, and Anatomy and Cell Biology, National Yang Ming University, Taiwan, People s Republic of China
    Clin Cancer Res 14:4085-95. 2008
    ..Identification and characterization of CSC from OSCC facilitates the monitoring, therapy, or prevention of OSCC...
  4. doi Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms
    David M Kristensen
    University Department of Growth and Reproduction, Section GR5064, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark
    Hum Reprod 23:775-82. 2008
    ....
  5. doi Pseudogene OCT4-pg4 functions as a natural micro RNA sponge to regulate OCT4 expression by competing for miR-145 in hepatocellular carcinoma
    Lei Wang
    State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi an, China
    Carcinogenesis 34:1773-81. 2013
    The POU transcription factor OCT4 is a pleiotropic regulator of gene expression in embryonic stem cells...
  6. doi Prognostication of OCT4 isoform expression in prostate cancer
    Marina França de Resende
    Department of Anatomic Pathology, A C Camargo Cancer Hospital, Rua Professor Antonio Prudente 211, Liberdade, Sao Paulo, SP, 01509 900, Brazil
    Tumour Biol 34:2665-73. 2013
    ..b>OCT4 is central to embryonic stem cell self-renewal and differentiation into specific lineages and encodes two chief ..
  7. doi Expression pattern of Oct-4, Sox2, and c-Myc in the primary culture of human dental pulp derived cells
    Lu Liu
    Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat Sen University, Guangzhou, Guangdong, China
    J Endod 37:466-72. 2011
    ....
  8. ncbi OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer
    Yaser Atlasi
    Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
    Int J Cancer 120:1598-602. 2007
    ..Our study demonstrated, for the first time, the expression of OCT-4 in bladder cancer and a further clue to the involvement of embryonic genes in carcinogenesis...
  9. pmc Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract
    Christel T Freberg
    Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 18:1543-53. 2007
    ..reprogramming of DNA methylation and histone modifications on regulatory regions of the developmentally regulated OCT4 and NANOG genes by exposure of 293T cells to EC cell extract...
  10. doi Oct-3/4 expression reflects tumor progression and regulates motility of bladder cancer cells
    Chao Ching Chang
    Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan
    Cancer Res 68:6281-91. 2008
    ..Therefore, our results implicate that Oct-3/4 may be useful as a novel tumor biological and prognostic marker and probably as a potential therapeutic target for bladder cancer...
  11. doi OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells
    Yaser Atlasi
    Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
    Stem Cells 26:3068-74. 2008
    b>OCT4 is a master regulator of self-renewal in embryonic stem cells and can potentially encode two spliced variants, designated OCT4A and OCT4B...
  12. doi MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells
    Na Xu
    Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, The University of California, Santa Barbara, Santa Barbara, CA 93106, USA
    Cell 137:647-58. 2009
    ..We identify the pluripotency factors OCT4, SOX2, and KLF4 as direct targets of miR-145 and show that endogenous miR-145 represses the 3' untranslated regions ..
  13. doi A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity
    Na Yu Chia
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672
    Nature 468:316-20. 2010
    ..profiling experiments revealed that PRDM14 colocalized extensively with other key transcription factors such as OCT4, NANOG and SOX2, indicating that PRDM14 is integrated into the core transcriptional regulatory network...
  14. doi Inhibition of LDH-A by lentivirus-mediated small interfering RNA suppresses intestinal-type gastric cancer tumorigenicity through the downregulation of Oct4
    Yongjie Zhang
    Department of Oncology, Changzheng Hospital, Shanghai, China
    Cancer Lett 321:45-54. 2012
    ..LDH-A reduction can suppress the tumorigenicity of intestinal-type gastric cancer (ITGC) cells by downregulating Oct4 both in vitro and in vivo...
  15. doi OCT4 regulates epithelial-mesenchymal transition and its knockdown inhibits colorectal cancer cell migration and invasion
    Xinzheng Dai
    Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing 210029, PR China
    Oncol Rep 29:155-60. 2013
    Octamer-binding transcription factor 4 (OCT4) has been implicated in cancer metastasis...
  16. ncbi Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency
    Lesley Gerrard
    Department of Gene Expression and Development, Roslin, Midlothian, UK
    Stem Cells 23:124-33. 2005
    ..these lines are able to express the enhanced green fluorescent protein (EGFP) reporter gene under control of the OCT4 promoter...
  17. pmc Glioma-initiating cells retain their tumorigenicity through integration of the Sox axis and Oct4 protein
    Hiroaki Ikushima
    Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    J Biol Chem 286:41434-41. 2011
    ..Takahashi, M., Miyazawa, K., and Miyazono, K. (2009) Cell Stem Cell 5, 504-514). Here we demonstrate that Oct4 plays essential roles in retention of the stemness properties of glioma-initiating cells through positive ..
  18. doi Oct4 and Sox2 are overexpressed in human neuroblastoma and inhibited by chemotherapy
    Shaobo Yang
    Department of Surgery, Children s Hospital, Fudan University, Shanghai 201102, PR China
    Oncol Rep 28:186-92. 2012
    ..b>Oct4 and Sox2 are essential transcription factors for embryonic development and play key roles in determining the fate ..
  19. doi Expression of the pluripotent transcription factor OCT4 promotes cell migration in endometriosis
    Jui Hung Chang
    Department of Biochemistry, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan
    Fertil Steril 99:1332-1339.e5. 2013
    To identify the impact of the pluripotent transcription factor OCT4 in endometrial cell migration and endometriosis.
  20. doi Positive correlation of Oct4 and ABCG2 to chemotherapeutic resistance in CD90(+)CD133(+) liver cancer stem cells
    Qian Jia
    Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China
    Cell Reprogram 15:143-50. 2013
    ..Expression of CD90, CD133, Oct4, and ABCG2 mRNA and protein levels was detected by PCR and western blot, respectively, which showed that these ..
  21. doi Downregulation of transcription factor Oct4 induces an epithelial-to-mesenchymal transition via enhancement of Ca2+ influx in breast cancer cells
    Jiajia Hu
    Medical School of Nankai University, Tianjin, China
    Biochem Biophys Res Commun 411:786-91. 2011
    The stem cell-related transcription factor Oct4 regulates tumor proliferation and apoptosis, but its role in tumor migration and invasion is still undefined...
  22. doi Oct4-induced pluripotency in adult neural stem cells
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, NRW, Germany
    Cell 136:411-9. 2009
    The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc...
  23. doi Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription
    Jungwoon Lee
    Department of Life Science, Sogang University, Seoul 121 742, Republic of Korea
    Int J Biochem Cell Biol 40:1043-54. 2008
    ..Moreover, ectopic expression of the PKM2 enhanced Oct-4-mediated transcription. These observations indicate that the transactivation potential of the Oct-4 transcription factor is positively modulated by PKM2...
  24. pmc Phosphorylation regulates human OCT4
    Justin Brumbaugh
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706 1532, USA
    Proc Natl Acad Sci U S A 109:7162-8. 2012
    The transcription factor OCT4 is fundamental to maintaining pluripotency and self-renewal...
  25. ncbi Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation
    Fang Wei
    Department of Animal Biology, Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:21551-60. 2007
    Transcription factor Oct4 is a master regulator affecting the fate of pluripotent stem cells and germ cell precursors...
  26. pmc Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells
    Ping Yuan
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Genes Dev 23:2507-20. 2009
    ..Coimmunoprecipitation and ChIP assays further demonstrate that Eset interacts with Oct4, which in turn recruits Eset to silence these trophoblast-associated genes...
  27. doi Expression profile of the embryonic markers nanog, OCT-4, SSEA-1, SSEA-4, and frizzled-9 receptor in human periodontal ligament mesenchymal stem cells
    Oriana Trubiani
    Department of Oral Science, University of Chieti Pescara, Chieti, Italy
    J Cell Physiol 225:123-31. 2010
    ..Taken together, these data indicate the presence of immature MSCs in PDL and suggest that the frizzled-9/Wnt pathway plays an important role in regulating proliferation and differentiation of these cells...
  28. pmc Expression and differentiation between OCT4A and its Pseudogenes in human ESCs and differentiated adult somatic cells
    Mojca Jež
    Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia
    PLoS ONE 9:e89546. 2014
    The POU5F1 gene codes for the OCT4 transcription factor, which is one of the key regulators of pluripotency...
  29. pmc Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers
    Attila Remenyi
    Gene Expression Program, EMBL, 69117 Heidelberg, Germany
    Genes Dev 17:2048-59. 2003
    ..In this study, we focus on two transcription factors, Oct4 and Sox2, as their combination on DNA is considered to direct the establishment of the first three lineages in the ..
  30. doi Upregulation and inhibition of the nuclear translocation of Oct4 during multistep gastric carcinogenesis
    Fathyia Y Al-Marzoqee
    Department of Anatomy, Faculty of Medicine and Health Sciences, UAE University, Al Ain, United Arab Emirates
    Int J Oncol 41:1733-43. 2012
    ..tissue stem cells and the self renewal transcription factor, octamer-binding transcription factor 4 (Oct4), could be involved in the development of certain tumors...
  31. doi Oct4 and Nanog expression is associated with early stages of pancreatic carcinogenesis
    Jing Wen
    Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea
    Pancreas 39:622-6. 2010
    To characterize the role of Oct4 and Nanog, two important homeobox transcription factors of embryonic development, in pancreatic carcinogenesis.
  32. doi Oct-3/4 promotes migration and invasion of glioblastoma cells
    Kana Kobayashi
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Toon, Ehime 791 0295, Japan
    J Cell Biochem 113:508-17. 2012
    ..Therefore, Oct-3/4 might contribute to tumor promoting activity in glioblastomas...
  33. doi Increased expression of OCT4 is associated with low differentiation and tumor recurrence in human hepatocellular carcinoma
    Zhongyi Dong
    Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
    Pathol Res Pract 208:527-33. 2012
    Octamer binding transcription factor 4 (OCT4), a key transcription factor required to maintain self-renewal and pluripotency of human and mouse embryonic stem cells, has been recently identified to be associated with tumorigenesis and ..
  34. pmc Human organic cation transporters 1 (SLC22A1), 2 (SLC22A2), and 3 (SLC22A3) as disposition pathways for fluoroquinolone antimicrobials
    Aditi Mulgaonkar
    Department of Pharmaceutics, Virginia Commonwealth University, Richmond, Virginia, USA
    Antimicrob Agents Chemother 57:2705-11. 2013
    ..However, hOCT1 in the sinusoidal membrane of hepatocytes, and potentially the basolateral membrane of proximal tubule cells, is likely to play a role in the disposition of these antimicrobial agents...
  35. ncbi Oct4 pseudogenes are transcribed in cancers
    Guangli Suo
    Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
    Biochem Biophys Res Commun 337:1047-51. 2005
    Octamer-binding transcription factor 4 (Oct4) plays a critical role for maintaining pluripotent and the self-renewing state of stem cells. Recent studies demonstrated that Oct4 gene was expressed in human cancers...
  36. ncbi Absence of OCT4 expression in somatic tumor cell lines
    Tobias Cantz
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, D 48149 Munster, Germany
    Stem Cells 26:692-7. 2008
    The POU-domain transcription factor OCT4 is associated with the pluripotent state of cells comprising the inner cell mass of pre-implantation embryos and has been known to play a critical role in the maintenance of pluripotency of ..
  37. pmc A data integration approach to mapping OCT4 gene regulatory networks operative in embryonic stem cells and embryonal carcinoma cells
    Marc Jung
    Molecular Embryology and Aging Group, Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 5:e10709. 2010
    ..Correlating gene expression levels after RNAi-based ablation of OCT4 function with its downstream targets enables a better prediction of motif-specific driven expression modules ..
  38. pmc OCT4 establishes and maintains nucleosome-depleted regions that provide additional layers of epigenetic regulation of its target genes
    Jueng Soo You
    Department of Urology, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
    Proc Natl Acad Sci U S A 108:14497-502. 2011
    ..endogenous DNA methylation and nucleosome occupancies on individual DNA molecules, we show that the unmethylated OCT4 distal enhancer has an NDR, whereas NANOG has a clear NDR at its proximal promoter...
  39. ncbi Phage display screening reveals an association between germline-specific transcription factor Oct-4 and multiple cellular proteins
    C Butteroni
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, D 69126, Germany
    J Mol Biol 304:529-40. 2000
    ..In addition, HMG-1 was found to cooperate with Oct-4 in P19 EC cells. These results provide the first evidence of a non-viral factor that enhances Oct-4 distance-dependent transactivation in stem cells...
  40. ncbi Oct-4 mRNA and protein expression during human preimplantation development
    G Cauffman
    Research Centre Genetics and Reproduction, University Hospital and Medical School, Brussels Free University, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
    Mol Hum Reprod 11:173-81. 2005
    ..Further, we suggest to examine the localization of OCT-4 proteins within a cell rather than to look for the presence and/or amount of transcripts...
  41. pmc Oct-4 expression maintained cancer stem-like properties in lung cancer-derived CD133-positive cells
    Yu Chih Chen
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 3:e2637. 2008
    ..Future research is warranted regarding the up-regulated expression of Oct-4 in LC-CD133(+) and malignant lung cancer...
  42. ncbi Morphological and molecular characterization of novel population of CXCR4+ SSEA-4+ Oct-4+ very small embryonic-like cells purified from human cord blood: preliminary report
    M Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 21:297-303. 2007
    ..Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties...
  43. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4...
  44. doi Klf4 interacts directly with Oct4 and Sox2 to promote reprogramming
    Zong Wei
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Stem Cells 27:2969-78. 2009
    ..b>Oct4, Sox2, and Klf4, factors that share many target genes in embryonic stem (ES) cells, are critical components in ..
  45. ncbi Oct-4 expression in human endometrium
    C Matthai
    Department of Gynecological Endocrinology and Reproductive Medicine, Medical University of Vienna, Waehringer Guertel, Vienna, Austria
    Mol Hum Reprod 12:7-10. 2006
    ..Oct-4 mRNA was detected by RT-PCR in all tested samples (9 of 9) of endometrium, although the levels of expression varied. To our knowledge, this is the first study demonstrating Oct-4 expression in human endometrium...
  46. doi Prognostic significance of OCT4 expression in adenocarcinoma of the lung
    Xueyan Zhang
    Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, NO 241, West Huaihai Rd, Shanghai 200030, People s Republic of China
    Jpn J Clin Oncol 40:961-6. 2010
    ..the presence of cancer stem-like cells with bronchioalveolar stem cells (BASCs) properties and investigate the clinicopathological role of expression of OCT4 as well as the correlation with clinical outcomes in adenocarcinoma of the lung.
  47. pmc The pluripotency factor Oct4 interacts with Ctcf and also controls X-chromosome pairing and counting
    Mary E Donohoe
    Howard Hughes Medical Institute, USA
    Nature 460:128-32. 2009
    ..Here we show that Oct4 (also known as Pou5f1) lies at the top of the XCI hierarchy, and regulates XCI by triggering X-chromosome pairing ..
  48. ncbi Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells
    Maryam M Matin
    The Centre for Stem Cell Biology, University of Sheffield, Western Bank, S10 2TN, U K
    Stem Cells 22:659-68. 2004
    We have used RNA interference (RNAi) to downregulate beta2-microglobulin and Oct4 in human embryonal carcinoma (hEC) cells and embryonic stem (hES) cells, demonstrating that RNAi is an effective tool for regulating specific gene activity ..
  49. ncbi Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells
    Yasmin Babaie
    Roslin Institute, Department of Gene Function and Development, Roslin, Midlothian, United Kingdom
    Stem Cells 25:500-10. 2007
    The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst...
  50. pmc Silencing of core transcription factors in human EC cells highlights the importance of autocrine FGF signaling for self-renewal
    Boris Greber
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Berlin, Germany
    BMC Dev Biol 7:46. 2007
    ..To obtain further insights into the regulation of self-renewal, we have silenced hES/hEC cell-specific genes in NCCIT hEC cells and analysed the downstream effects by means of microarrays...
  51. ncbi Differentiation of human embryonic stem cells induces condensation of chromosome territories and formation of heterochromatin protein 1 foci
    Eva Bartova
    Laboratory of Molecular Cytology, Cytometry Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65, Brno, Czech Republic
    Differentiation 76:24-32. 2008
    ..Nuclear patterns of the Oct4 (6p21.33) gene, responsible for hES cell pluripotency, the C-myc (8q24...
  52. doi OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs)
    Kwang Won Seo
    Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
    Biochem Biophys Res Commun 384:120-5. 2009
    ..A decreased rate of cell proliferation was observed in OCT4-inhibited hUCB-MSCs...
  53. ncbi Oct-1 preferentially interacts with androgen receptor in a DNA-dependent manner that facilitates recruitment of SRC-1
    M I Gonzalez
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    J Biol Chem 276:6420-8. 2001
    ....
  54. ncbi Transcriptional regulation of human Oct4 by steroidogenic factor-1
    Heung Mo Yang
    Chabiotech Co Ltd, Seoul, South Korea
    J Cell Biochem 101:1198-209. 2007
    b>Oct4 encodes a transcription factor that is involved in the maintenance of self-renewal in stem cells. Recently, the molecular mechanisms that regulate Oct4 expression have come under investigation...
  55. doi Independent prognostic value of the basal-like phenotype of breast cancer and associations with EGFR and candidate stem cell marker BMI-1
    J B Arnes
    The Gade Institute, Section for Pathology, University of Bergen, Haukeland University Hospital, Bergen, Norway
    Histopathology 52:370-80. 2008
    ..To study the relationship between basal-like breast cancers, epidermal growth factor receptor (EGFR) and candidate stem cell markers (BMI-1, EZH2, Oct-4) in a population-based setting...
  56. doi Mutation in the DNA-binding domain of the EWS-Oct-4 oncogene results in dominant negative activity that interferes with EWS-Oct-4-mediated transactivation
    Sol Kim
    Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul, Korea
    Int J Cancer 124:2312-22. 2009
    ..Thus, this mutation in the POU DNA-binding domain results in a dominant negative protein. These findings suggest that the biological functions of the EWS-Oct-4 oncogene can be modulated by the dominant negative mutant EWS-Oct-4 (V351P)...
  57. ncbi In situ hybridization localizes the human OTF3 to chromosome 6p21.3-->p22 and OTF3L to 12p13
    T Guillaudeux
    LIGHT INSERM CNRS, Toulouse, France
    Cytogenet Cell Genet 63:212-4. 1993
    ..We report the mapping of the human homologue: OTF3, to human chromosome 6p21.3-->p22 within or close to the human MHC class I region...
  58. ncbi Expression of transcription factor Oct-4 and other embryonic genes in CD133 positive cells from human umbilical cord blood
    Nelli Baal
    Department of Obstetrics, Justus Liebig University, Giessen, Germany
    Thromb Haemost 92:767-75. 2004
    ..Our results indicate that CD133(+) HSPC from umbilical cord blood may have a greater differentiation potential than previously recognized and give rise to proliferative endothelial cells participating in placental vasculogenesis...
  59. ncbi High-efficiency RNA interference in human embryonic stem cells
    Holm Zaehres
    Harvard Stem Cell Institute and Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology and Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
    Stem Cells 23:299-305. 2005
    ..silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells...
  60. ncbi Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker
    Stefano Zangrossi
    Cell Factory, Center for Transfusion Medicine, Cellular Therapy and Cryobiology, Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Via F Sforza 35, 20122 Milano, Italy
    Stem Cells 25:1675-80. 2007
    The Oct-4 transcription factor, a member of the POU family that is also known as Oct-3 and Oct3/4, is expressed in totipotent embryonic stem cells (ES) and germ cells, and it has a unique role in development and in the determination of ..
  61. ncbi Manipulation of OCT4 levels in human embryonic stem cells results in induction of differential cell types
    Ryan T Rodriguez
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Exp Biol Med (Maywood) 232:1368-80. 2007
    ..To evaluate the role of OCT4 in the regulation of hESC self-renewal and differentiation, a vector expressing a hairpin RNA targeting endogenous ..
  62. pmc Oct4A is expressed by a subpopulation of prostate neuroendocrine cells
    Paula Sotomayor
    Department of Urologic Oncology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Prostate 69:401-10. 2009
    ..and pluripotency in embryonic stem cells and germ cells are regulated by Oct4A, a splice variant of the POU5F1 (Oct3/4) gene, while the function of the alternative splice variant, Oct4B, is unknown...
  63. doi The expression of pluripotency marker Oct 3/4 in prostate cancer and benign prostate hyperplasia
    Nastaran Monsef
    Division of Pathology, Department of Laboratory Medicine, Lund University Hospital, Lund, Sweden
    Prostate 69:909-16. 2009
    b>Oct 3/4 (Octamer 3/4), a member of POU family has been considered as an important stem cell marker and essential transcription factor during human embryogenesis...
  64. doi Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response
    Xia Wang
    Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
    Stem Cells 27:1265-75. 2009
    b>OCT4 is a pivotal transcription factor in maintaining the pluripotency and self-renewal capacities of embryonic stem (ES) cells. Human OCT4 can generate two isoforms by alternative splicing, termed OCT4A and OCT4B...
  65. pmc Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues
    J Takeda
    Howard Hughes Medical Institute, Chicago, IL
    Nucleic Acids Res 20:4613-20. 1992
    ..This approach resulted in the amplification of sequences encoding the octamer binding proteins Oct1 and Oct3 (also called Oct4)...
  66. ncbi Expression of transcription regulating genes in human preimplantation embryos
    B Abdel-Rahman
    Department of Obstetrics and Gynecology, Northwestern University Medical School, Prentice Women s Hospital and Maternity Center, Chicago, Illinois, USA
    Hum Reprod 10:2787-92. 1995
    ..chain reaction (RT-PCR) assay system, the time course of mRNA expression of two transcription regulators, OCT4 and OCT6, was assessed in individual preimplantation human embryos...
  67. ncbi Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4
    J Nichols
    Centre for Genome Research, University of Edinburgh, United Kingdom
    Cell 95:379-91. 1998
    b>Oct4 is a mammalian POU transcription factor expressed by early embryo cells and germ cells. We report that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo...
  68. pmc The embryonic stem cell transcription factors Oct-4 and FoxD3 interact to regulate endodermal-specific promoter expression
    Ying Guo
    Department of Medicine and the Walther Oncology Center, R4 202, 1044 West Walnut Street, Indiana University Medical Center, Indianapolis, IN 46202, USA
    Proc Natl Acad Sci U S A 99:3663-7. 2002
    ..These data indicate that Oct-4 functions as a corepressor of FoxD3 to provide embryonic lineage-specific transcriptional regulatory activity to maintain appropriate developmental timing...
  69. ncbi POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors
    Leendert H J Looijenga
    Pathology Lab for Exp Patho Oncology, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Cancer Res 63:2244-50. 2003
    ..OCT3/4, a transcription factor also known as OTF3 and POU5F1, is involved in regulation of pluripotency during normal development and is detectable in embryonic stem ..
  70. ncbi Developmental expression of POU5F1 (OCT-3/4) in normal and dysgenetic human gonads
    Ewa Rajpert-De Meyts
    Department of Growth and Reproduction, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
    Hum Reprod 19:1338-44. 2004
    ....
  71. ncbi Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis
    Mei Hui Tai
    National Food Safety Toxicology Center, Department of Pediatrics and Human Development, Michigan State University, East Lansing, MI 48824, USA
    Carcinogenesis 26:495-502. 2005
    The Oct3/4 gene, a POU family transcription factor, has been noted as being specifically expressed in embryonic stem cells and in tumor cells but not in cells of differentiated tissues...
  72. ncbi EWSR1 is fused to POU5F1 in a bone tumor with translocation t(6;22)(p21;q12)
    Shuichi Yamaguchi
    Department of Carcinogenesis, The Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan
    Genes Chromosomes Cancer 43:217-22. 2005
    POU5F1(OCT3/4) is a sequence-specific transcription factor that is essential for keeping germ cells and embryonic stem cells in an immature and pluripotent status...
  73. ncbi Stimulation of Oct-4 activity by Ewing's sarcoma protein
    Jungwoon Lee
    Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul 121 742, Korea
    Stem Cells 23:738-51. 2005
    ..Taken together, our results suggest that Oct-4-mediated transactivation is stimulated by EWS...
  74. pmc Core transcriptional regulatory circuitry in human embryonic stem cells
    Laurie A Boyer
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Cell 122:947-56. 2005
    The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture...
  75. ncbi POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos
    Greet Cauffman
    Research Center Reproduction and Genetics, University Hospital and Medical School of the Free University of Brussels, Laarbeeklaan 101, 1090 Brussels, Belgium
    Stem Cells 24:2685-91. 2006
    ..The expression pattern of POU5F1_iA may suggest that POU5F1_iA alone cannot sustain totipotency and that coexpression with other stemness factors might be the key to totipotency...
  76. ncbi The human OCT-4 isoforms differ in their ability to confer self-renewal
    Jungwoon Lee
    Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul 121 742, Korea
    J Biol Chem 281:33554-65. 2006
    ..Taken together, these data suggest that the DNA binding, transactivation, and abilities to confer self-renewal of the human OCT-4 isoforms differ...
  77. ncbi Oct-3/4 dose dependently regulates specification of embryonic stem cells toward a cardiac lineage and early heart development
    Dana Zeineddine
    CNRS FRE2593, CRBM, 34293 Montpellier, France
    Dev Cell 11:535-46. 2006
    ..Thus, quantitative Oct-3/4 expression is regulated by a morphogen, pointing to a pivotal and physiological function of the POU factor in mesodermal and cardiac commitments of ES cells and of the epiblast...
  78. ncbi Oct-4, Rex-1, and Gata-4 expression in human MSC increase the differentiation efficiency but not hTERT expression
    Stephane Roche
    Centre d investigation Biologique, Centre Hospitalier Universitaire de Grenoble, Grenoble, France
    J Cell Biochem 101:271-80. 2007
    ..Our data suggest that the culture medium used permits the emergence of adult stem cells with a high differentiation capacity and expression of embryonic factors. These cells may have important implications for cell therapy...
  79. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
    ..We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the ..
  80. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..fibroblasts have been reprogrammed directly to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4 and Myc) to yield induced pluripotent stem (iPS) cells...
  81. doi Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development
    Marilyn Monk
    Molecular Embryology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Mol Hum Reprod 14:347-55. 2008
    ..Their expression profiles are compared with that of the well-known pluripotency gene, OCT4, using a primer design that avoids amplification of the multiple OCT4 pseudogenes...
  82. doi Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 454:646-50. 2008
    ..as induced pluripotent stem (iPS) cells, has been possible with the expression of the transcription factor quartet Oct4 (also known as Pou5f1), Sox2, c-Myc and Klf4 (refs 1-11)...
  83. doi In vivo osteoprogenitor potency of human stromal cells from different tissues does not correlate with expression of POU5F1 or its pseudogenes
    Nikolas Kaltz
    Third Department of Internal Medicine, Klinikum rechts der Isar, Technische Universitat Munchen, Munchen, Germany
    Stem Cells 26:2419-24. 2008
    ..Despite low-level expression of POU5F1, we were unable to precipitate POU5F1 protein in either BM-MSC or UVSC. These results demonstrate that MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes...
  84. pmc Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells
    Deborah A Greer Card
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 28:6426-38. 2008
    b>Oct4 and Sox2 are transcription factors required for pluripotency during early embryogenesis and for the maintenance of embryonic stem cell (ESC) identity...
  85. pmc Novel variants of Oct-3/4 gene expressed in mouse somatic cells
    Nobuhiko Mizuno
    Research Unit for Cell Plasticity, RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    J Biol Chem 283:30997-1004. 2008
    ..Furthermore, we determined novel promoter regions that are sufficient for the expression of Oct-3/4 transcript variants in somatic cells. These findings provide new insights into the postnatal role of Oct-3/4 in somatic tissues...
  86. doi MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation
    Yvonne Tay
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Agency for Science Technology and Research, 08 01, Genome, 60 Biopolis Street, Singapore 138672, Singapore
    Nature 455:1124-8. 2008
    ..Here we focus on the mouse Nanog, Oct4 (also known as Pou5f1) and Sox2 genes and demonstrate the existence of many naturally occurring miRNA targets in ..
  87. pmc Induced pluripotent stem cells generated without viral integration
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Science 322:945-9. 2008
    ..stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc...
  88. doi Oct4 is expressed in human gliomas and promotes colony formation in glioma cells
    Zhanhui Du
    Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University School of Medicine, Jinan, China
    Glia 57:724-33. 2009
    ..Here, we reported that Oct4, a well-known regulator of self-renewal in embryonic stem cells, was highly expressed in human gliomas and glioma ..
  89. doi Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells
    T Mueller
    Department of Internal Medicine IV, Oncology Hematology, Martin Luther University Halle Wittenberg, Ernst Grube Str 40, 06120 Halle, Germany
    Cell Mol Life Sci 66:495-503. 2009
    b>OCT4 is considered a main regulator of embryonic stem cell pluripotency and self renewal capacity. It was shown that relevant OCT4 expression only occurs in cells of embryonic pluripotent nature...
  90. pmc Genome-wide analysis reveals Sall4 to be a major regulator of pluripotency in murine-embryonic stem cells
    Jianchang Yang
    Division of Laboratory Medicine, Nevada Cancer Institute, One Breakthrough Way, Las Vegas, NV 89135, USA
    Proc Natl Acad Sci U S A 105:19756-61. 2008
    ..Sall4, a zinc-finger transcription factor, is expressed very early in embryonic development with Oct4 and Nanog, two well-characterized pluripotency regulators...
  91. doi Identification of karyopherin-alpha 2 as an Oct4 associated protein
    Xiangqun Li
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    J Genet Genomics 35:723-8. 2008
    The POU domain transcription factor Oct4 is a master regulator in maintaining self-renewal and pluripotency of embryonic stem (ES) cells...
  92. doi OCT4 expression in human non-small cell lung cancer: implications for therapeutic intervention
    Golnaz Karoubi
    Division of General Thoracic Surgery, University Hospital Berne, Berne, Switzerland
    Interact Cardiovasc Thorac Surg 8:393-7. 2009
    Here we investigate the expression of OCT4 human lung adenocarcinoma and bronchioloalveolar carcinoma (BAC) tumor biopsies and tumor-derived primary cell cultures...
  93. pmc A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress
    Jinsuk Kang
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Genes Dev 23:208-22. 2009
    Oct1 and Oct4 are homologous transcription factors with similar DNA-binding specificities. Here we show that Oct1 is dynamically phosphorylated in vivo following exposure of cells to oxidative and genotoxic stress...
  94. doi Oct4, a novel marker for human gastric cancer
    Zhong Chen
    School of Medical Science and Laboratory Medicine, Key Institute of Clinical Laboratory Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China
    J Surg Oncol 99:414-9. 2009
    Octamer-4 (Oct4), a transcription factor involved in regulating human embryonic stem cells (ESCs), may play a role in tumorigenesis...
  95. ncbi Oct3/4 and Sox2 are significantly associated with an unfavorable clinical outcome in human esophageal squamous cell carcinoma
    Qi Wang
    Department of Pathology, The Norwegian Radium Hospital, Oslo, Norway
    Anticancer Res 29:1233-41. 2009
    The embryonic stem cell factors Oct3/4 and Sox2 are essential for pluripotency and self-renewal of embryonic stem cells...
  96. pmc Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation
    Eirini P Papapetrou
    Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:12759-64. 2009
    ..Using this system, we define the optimal stoichiometry of RF expression to be highly sensitive to Oct4 dosage, and we demonstrate the impact that variations in the relative ratios of RF expression exert on the ..
  97. doi Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS
    Hatsune Makino
    Department of Reproductive Biology, National Institute for Child Health and Development, Tokyo, 157 8535, Japan
    Exp Cell Res 315:2727-40. 2009
    POU5F1 (more commonly known as OCT4/3) is one of the stem cell markers, and affects direction of differentiation in embryonic stem cells...
  98. doi Correlation of CD133, OCT4, and SOX2 in rectal cancer and their association with distant recurrence after chemoradiotherapy
    Susumu Saigusa
    Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie, Japan
    Ann Surg Oncol 16:3488-98. 2009
    ..We investigated the role of three stem cell markers (CD133, OCT4, and SOX2) in rectal cancer and evaluated the association between these gene levels and clinical outcome in rectal ..
  99. doi Direct reprogramming of human neural stem cells by OCT4
    Jeong Beom Kim
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 461:649-3. 2009
    ..cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4)...
  100. pmc Direct cell reprogramming is a stochastic process amenable to acceleration
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nature 462:595-601. 2009
    Direct reprogramming of somatic cells into induced pluripotent stem (iPS) cells can be achieved by overexpression of Oct4, Sox2, Klf4 and c-Myc transcription factors, but only a minority of donor somatic cells can be reprogrammed to ..
  101. doi Wwp2 mediates Oct4 ubiquitination and its own auto-ubiquitination in a dosage-dependent manner
    Bing Liao
    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai Jiao Tong University School of Medicine 225 South Chongqing Road, Shanghai 200025, China
    Cell Res 20:332-44. 2010
    Transcription factor Oct4 plays critical roles in maintaining pluripotency and controlling lineage commitment of embryonic stem cells (ESCs)...

Research Grants70

  1. TSHR and thyrocyte development
    Reigh Yi Lin; Fiscal Year: 2013
    ..fibroblasts into an ES cell-like state using the retrovirus-mediated transfection of four transcription factors (Oct4, Sox2, c-Myc, and Klf4)...
  2. Herman S Cheung; Fiscal Year: 2014
    ..Our recent study showed that cells isolated from adult human PDL expressed ESC markers ( Oct4, Nanog, Sox2) and NC markers (p75, Nestin, Sox10), and exhibited the potential to differentiate into all 3 germ ..
  3. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ..of redundant epigenetic regulatory components and * significant up-regulation of the key pluripotency gene Oct4 with combined Epi-drug and proteasome inhibitor * treatment...
  4. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..of induced pluripotent stem (iPS) cells by reprogramming of somatic cells via the defined transcription factors (Oct3/4 and Sox2 with Klf4 and c-Myc or Nanog and Lin28)...
  5. Function of KLF4-expressing stem cells in mouse cutaneous wound healing
    Walden Ai; Fiscal Year: 2013
    ..and induction of induced pluripotent stem cells from well-differentiated fibroblasts with three other proteins, Oct4, c-myc, and Sox2...
  6. Mana M Parast; Fiscal Year: 2014
    ..from these molar placentas and reprogram them into iPS cells using lenti- or Sendai virus expressing Klf4, Sox2, Oct4, and c-Myc...
  7. Cardiovascular and Therapeutic Potential of Reprogrammed Human Fibroblasts
    WILLIAM ROBB MACLELLAN; Fiscal Year: 2010
    ..Simultaneous overexpression of the pluripotency-associated POU domain class 5 transcription factor 1 (Oct3/4), SRY-box containing gene 2 (Sox2), proto-oncogene myc (c-Myc), and Kruppel-like factor 4 (Klf4) led to the ..
  8. Marian L Waterman; Fiscal Year: 2016
    ..TCF3, is a transcription factor that in mouse ESCs (mESCs) represses expression of master pluripotency factors Oct4, Sox2, and Nanog (OSN)...
  9. Ming Ming Zhou; Fiscal Year: 2014
    ..Yamanaka's reprogramming of somatic cells to induced pluripotent stem cells using four transcription factors Oct3/4, Sox2, Klf4 and c-Myc (OSKM) highlights the ultimate control of cell fate by gene transcription...
  10. Recapitulating developmental pathways to improve iPSC-RPE derivation protocols
    PETER DWAYNE WESTENSKOW; Fiscal Year: 2013
    ..derived RPE from hESCs and iPSCs induced to pluripotency using all 4 Thomson factors and with only two (Klf4 and Oct4) and biomicking small molecules...
  11. Aging and Reprogramming of Somatic Cells to Pluripotency
    Peter J Hornsby; Fiscal Year: 2010
    ..This will be tested by the retroviral introduction of reprogramming genes (Oct4, Sox2, Klf4, with or without c-Myc) into skin fibroblasts from marmosets of a range of ages, newborn to very old (~..
  12. Genomic-wide Analysis of Oct 3/4 and Nanog Targets
    Soren Impey; Fiscal Year: 2010
    ..The homeodomain transcription factors, Oct3/4 and Nanog, are believed to play critical roles in sustaining ES cell pluripotency...
  13. A Myc-centered network in embryonic stem cells and somatic cell reprogramming
    Jonghwan Kim; Fiscal Year: 2013
    ..Myc is one of the four transcription factors (Oct4, Sox2, Klf4, and Myc) used in generation of IPS cells and is a protein of interest due to its dual roles in the ..
  14. Generation of hPSCs Using Reprogramming Proteins-Encapsulated Nanoparticles
    Hsian Rong Tseng; Fiscal Year: 2012
    ..These NPs are capable of delivering reprogramming transcription factors (TFs), namely OCT4, SOX2, KLF4 and c-MYC...
  15. Optimal Generation and Characterization of iPS Cell Lines from Healthy and ADHD S
    Kwang Soo Kim; Fiscal Year: 2012
    ..e., Oct4, Sox2, Klf4 and c-Myc)...
  16. Optimal Generation and Characterization of iPS Cell Lines from Healthy and ADHD S
    Kwang Soo Kim; Fiscal Year: 2010
    ..e., Oct4, Sox2, Klf4 and c-Myc)...
  17. JAMES ALEXANDER THOMSON; Fiscal Year: 2015
    ..Transcription Factors for Reprogramming/Transdifferentation," aims are: Design ATFs that activate expression of Oct4, Sox2 or Nanog in primary fibroblasts;Perform genome-wide location analysis, CSI and transcriptome analysis to ..
  18. Matthew Thomson; Fiscal Year: 2016
    ..factors controls the ES cell, but, in previous research, I showed that a circuit of just two transcription factors, Oct4 and Sox2, controls germ layer fate selection...
  19. David L Spector; Fiscal Year: 2016
    ..The nuclear position of a series of 4 genes encoding the transcription factors Oct4, Nanog, Sox2, and Klf4 will be examined, as transcriptional silencing of these genes serves as a trigger for the ..
  20. ROBERT MICHAEL ROBERTS; Fiscal Year: 2016
    ..As the pig is an important reservoir for Salmonella, the LDLR-/- model will have a dual purpose for both agricultural and biomedical applications. ..
  21. Max A Cayo; Fiscal Year: 2016
    ..Methods: We generate iPSC lines from human fibroblasts by lentiviral transduction (vectors encoding Oct4, Nanog, Sox2, Lin28) and validate the resulting iPSC by immunostaining/fluorescence microscopy, flow cytometry, DNA ..
  22. Role of Brg1 in Embryo Development and Pluripotency
    JASON GLENN KNOTT; Fiscal Year: 2013
    ..specific aims of this application are to test the hypotheses that: 1) Brg1 is required for epigenetic silencing of Oct4 and Nanog expression in the trophectoderm...
  23. Jeffrey H Kordower; Fiscal Year: 2016
    ..e., Oct4, Sox2, Klf4 and c-Myc)(Takahashi and Yamanaka, 2006)...
  24. TcdB Based Protein Delivery of iPSC Reprogramming Factors
    Yung Nien Chang; Fiscal Year: 2011
    ..We will then test if the engineered TcdB can deliver biologically active reprogramming factors (Oct4 and Sox2) to another reporter cell line and assess their ability to differentiate mouse embryonic stem cells with ..
  25. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2012
    ..to become pluripotent through transduction with a retroviral vector carrying a cocktail of two embryonic genes, Oct4 and Sox2, and two cancer genes, Klf4 and c-myc...
  26. Patricia Devaux; Fiscal Year: 2014
    ..that measles virus can be modified into "one cycle" reprogramming vectors expressing the four reprogramming genes (OCT4, KFL4, SOX2 and cMYC)...
  27. A novel protein transduction method to generate induced pluripotent stem cells wi
    JAMES FREDERICK ELIASON; Fiscal Year: 2010
    ..The initial methods for reprogramming focused on delivery of four genes (Oct4, Sox2, and either Klf4 plus c-Myc, or NANOG plus Lin 28) using genetic transduction methods that potentially can ..
  28. Development of High Content Screens for Human Pluripotent Stem Cells
    April D Pyle; Fiscal Year: 2011
    ..We will develop an OCT4-EGFP reporter line as OCT4 marks pluripotent stem cells and gets rapidly down regulated upon differentiation, as ..
  29. Novel Roles of Pluripotency Regulators in the Early Mouse Embryo
    Wing H Wong; Fiscal Year: 2012
    ..We discovered that Oct4, a homeodomain transcription factor of the POU family that is known for its critical functions in pluripotency in ..
  30. Generation of hESC reporter lines using improved gene targeting technology
    TOBIAS DIETMAR RAABE; Fiscal Year: 2010
    ..First, we will target OCT4/POU5F1, a gene that is essential for maintenance of the undifferentiated state of hESC...
  31. Random shRNA Selection
    Robert B Wilson; Fiscal Year: 2012
    ..Reporter constructs for Nanog and Oct4 will be used to identify shRNAs for stem-cell induction, and reporter constructs for NKX2-5 and Ngn3 will be used ..
  32. Defining factors associated with the conversion of primordial germ cells into plu
    Candace Kerr; Fiscal Year: 2009
    ..in other species;(3) those containing sequence elements that may be responsive to known pluripotent factors such as Oct4, Nanog, or Sox2;and (4) finally, pathways shown to be relevant in pluripotent or multipotent cells...
  33. Development of a cell replacement therapy to treat retinal degenerative blindness
    BUDD ALBERT TUCKER; Fiscal Year: 2010
    ..produced via genetic reprogramming of dermal fibroblasts to pluripotency using the 4 transcription factors Oct4, Sox2, KLF4, and c-Myc, is of interest...
  34. Elaine Fuchs; Fiscal Year: 2016
    ..cultured embryonic stem cells (ESCs), Tcf3 is an integral component of the pluripotency regulatory complex (Sox2, Oct4, Nanog), not present in adult SCs...
  35. Investigation of the Tip60-p400 complex role in embryonic stem cell self-ren
    Barbara Panning; Fiscal Year: 2012
    ..Several transcription factors, such as Oct4, Nanog, Sox2, Foxd3, and Stat3, are essential for self-renewal and pluripotency...
  36. Christina Tenenhaus Dann; Fiscal Year: 2016
    ..Here we test a strategy for overcoming these impediments through manipulation of the transcription factor, OCT4, while examining fundamental questions of SSC regulation...
  37. Development of Pluripotent Amniotic Stem Cell Banks for Disease Modeling
    JAMES FREDERICK ELIASON; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Direct reprogramming of somatic cells with the transcription factors Oct4 (also called Pou5f1), Sox2, Klf4 and Myc1 yields induced Pluripotent Stem (iPS) cells with marked similarity to Embryonic Stem ..
  38. Jianlong Wang; Fiscal Year: 2015
    ..The pluripotent ESC identity is governed by a set of transcription factors centered on Oct4, Sox2 and Nanog together with a number of epigenetic regulators...
  39. TGF-b family signaling in cardiomyocyte differentiation from embryonic stem cells
    RIK M DERYNCK; Fiscal Year: 2010
    ..b>Oct4, Sox2 and Nanog function as essential transcription factors for self-renewal and pluripotency, yet may also play ..
  40. Pou6f1 transcriptional control of memory CD8+ T cells
    CHANCE MARION JOHN LUCKEY; Fiscal Year: 2012
    ..family of transcription factors and is a paralog of the embryonic stem cell self-renewal regulator Pou5f1 (aka Oct4). We believe Pou6f1 functions in memory CD8+ T cells in a manner similar to Oct4 in embryonic stem cells...
  41. A Myc-centered network in embryonic stem cells and somatic cell reprogramming
    Jonghwan Kim; Fiscal Year: 2010
    ..Myc is one of the four transcription factors (Oct4, Sox2, Klf4, and Myc) used in generation of iPS cells and Is a protein of interest due to its dual roles In the ..
  42. Generation of pluripotent cells by reprogramming of the somatic epigenome.
    GORDON GRANT WELSTEAD; Fiscal Year: 2010
    ..The recent discovery that four transcription factors: Oct4, Sox2, c-Myc and Klf4, can reprogram somatic fibroblasts into an embryonic-like pluripotent state has provided a ..
  43. Sheng Ding; Fiscal Year: 2014
    ..g. Oct4, cMyc, Sox2 and Klf4)...
  44. CHANCE MARION JOHN LUCKEY; Fiscal Year: 2014
    ..Pou6f1 is a member of the Pou-domain family of transcription factors and is a paralog of Pou5f1 (aka Oct4)...
  45. Amy Ralston; Fiscal Year: 2016
    ..The transcription factors Oct4 (Pou5f1) and Sox2 are necessary for pluripotency in ES cells and are key regulators of induced pluripotency...
  46. Hannele Ruohola-Baker; Fiscal Year: 2014
    ..The de-differentiated cells re-activate Oct4 promoter analyzed by Oct4- GFP...
  47. John J Laterra; Fiscal Year: 2016
    ..Specific transcription factors (TFs) such as Sox2, Oct4, and Nanog, have essential roles in maintaining the stemness and proliferation potential of normal neural stem ..
  48. KEVIN C EGGAN; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): The combined activity of four transcription factors (Oct4, Sox2, cMyc and Klf4) can reprogram adult cells into induced pluripotent stem (iPS) cells...
  49. Zhongjie Sun; Fiscal Year: 2016
    ..CIPH, cold exposure [unreadable] PBEF[unreadable] [unreadable] AMPK[unreadable]1[unreadable], PDE1B[unreadable], Oct4[unreadable] [unreadable] CIPH...
  50. Epigenetic modification of endothelial progenitor cells into multipotent cells
    Rajasingh Johnson; Fiscal Year: 2010
    ..We report that 1) 5Aza and TSA treatment of EPC induces pluripotent genes Oct4, and Nanog and down regulates endothelial cell lineage specific genes...
  51. Nabora Soledad Reyes de Mochel; Fiscal Year: 2014
    ..networks that control lineage specification during blastulation: the pluripotent regulatory motifs of Oct4/Nanog/Sox2 and the TE committing networks of Tead4, Cdx2, and Gata6...
  52. Small molecules that induce/promote generation of iPS cells
    Sheng Ding; Fiscal Year: 2010
    ..e. Oct4, cMyc, Sox2 and Klf4) together [5]...
  53. Epigenetics, Stem Cells, and Cancer
    Rudolf Jaenisch; Fiscal Year: 2010
    ..We have shown that activation of Oct4 in cells of the adult mouse leads to a rapid and fully reversible dysplastic proliferation of the intestinal and ..
  54. Shoukhrat M Mitalipov; Fiscal Year: 2014
    ..will examine a cumulative impact of the TE inactivation along with upregulation of critical pluripotent factors - Oct4, Sox2, and Nanog - on reprogramming and derivation of ESCs. Aim 2...
  55. Whole Genome Chromatin Interaction Analysis using Pair-End-diTagging (CIA-PET)
    Yijun Ruan; Fiscal Year: 2010
    ..b) Tertiary networks of transcription regulation mediated by Nanog and Oct4 in mouse embryonic stem cells. c) Estrogen receptor mediated chromatin interactions in human breast cancer cells...
  56. Characterization of Endoderm Stem Cells Derived from Murine ESCs
    Jon S Odorico; Fiscal Year: 2010
    ..The EndSCs are OCT4+ and express some early endoderm-restricted genes, whereas FGSCs are OCT4- and have high expression of Sox17, Foxa2,..
  57. New Multiplexed Quantitative Detection of Pluripotency and Germ Layer Proteins
    Bradley H Garcia; Fiscal Year: 2011
    ..by identifying matched pairs of antibodies (working sandwich assays) for four key pluripotency targets: Sox2, Oct4, Nanog and Rex1...
  58. Dieter C Gruenert; Fiscal Year: 2015
    ..IPS cells using phiC31 Integrase-mediated, sequence-specific integration of a plasmid carrying 2A peptide linked Oct4, Sox2, Klf4, and cMyc reprogramming cDNAs or by using small activating double stranded RNA (saRNA) to transiently ..
  59. Transduction of cells with transcription factors to achieve nuclear reprogramming
    Hidde Ploegh; Fiscal Year: 2009
    ..We then propose to install at the N-or C-terminus of the transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4 a cell-penetrating entity, to allow cytoplasmic and nuclear delivery of these ..
  60. Histocompatible Primate Embryonic Stem Cells
    Shoukhrat M Mitalipov; Fiscal Year: 2013
    ..monkey skin cells and test our working hypothesis that experimental upregulation of critical pluripotent factors - OCT4, SOX2, NANOG and CARM1- in cytoplasts will enhance reprogramming and increase the current efficiency of SCNT embryo ..
  61. Examining the Therapeutic Potential of iPS cells in Duchenne Muscular Dystrophy
    Rita C R Perlingeiro; Fiscal Year: 2010
    ..breakthrough of direct reprogramming adult mouse and human fibroblasts toward a pluripotent state by introducing Oct3/4, Sox2, Klf4, and c-Myc, has brought new hope for the generation of patient- and disease- specific stem cells ..
  62. Li na Wei; Fiscal Year: 2015
    ..These studies will employ embryonic stem (ES) cell culture as the experimental model system. Oct4 (Pou5f1) is the "master regulator" of pluriopotent stem cells including ES, and its expression level must be ..
  63. Ali H Brivanlou; Fiscal Year: 2014
    ..NIH registry), that has a single copy of two types of insertions: (i) a stable lentiviral insertion where the human Oct4 promoter drives the expression of EGFP and Neomycin (Neo), (ii) a mutagenic transposable element equipped with a ..
  64. Jonathan S Weissman; Fiscal Year: 2016
    ..Specifically, we will modify the epigenetic marks of the murine Oct4 and Sox2 loci as the endogenous experimental testbed, and study the importance of modifier recruitment positioning ..
  65. Guojun Wu; Fiscal Year: 2016
    ..technique that reprogrammed heterogeneous malignant Breast Cancer Cells (malBCCs) into piPSCs using the Sox2/Oct4/Nanog (SON) proteins with 90[unreadable]4% conversion...
  66. Liver Selective AMPK Activator to Treat Type 2 Diabetes
    KEN W BATCHELOR; Fiscal Year: 2013
    ..The recent discovery that a third OCT, OCT3, acts to transport metformin into skeletal muscle has enlarged the scope of this work...
  67. Michael A Dyer; Fiscal Year: 2016
    ..experimental system to quantify the epigenetic reprogramming of individual retinal neurons by using 4 factors (Oct4, Klf4, Sox2, and Myc) and somatic cell nuclear transfer...
  68. Mechanisms By Which Maternal And Fetal Drug Exposure Is*
    Jashvant Unadkat; Fiscal Year: 2006
    ..P-gp; Project 1), the Breast Cancer Resistance Protein (BCRP; Project 2), and the Organic Cation Transporter 3 (OCT3; Project 3)...
  69. Environmental Toxicants and Neurodegeneration
    Kim Tieu; Fiscal Year: 2010
    ..PQ) are bi- directionally transported across the astrocytic plasma membrane by the organic cation transporter 3 (OCT3) and, through this mechanism, OCT3 modulates neurotoxicity...
  70. ANALYSIS OF THE ROLE OF GCNF IN SPERMATOGENESIS
    Austin Cooney; Fiscal Year: 2003
    ..We have shown that GCNF can regulate the OCT4 transcription factor gene...