p67phox

Summary

Gene Symbol: p67phox
Description: neutrophil cytosolic factor 2
Alias: NCF-2, NOXA2, P67-PHOX, P67PHOX, neutrophil cytosol factor 2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, chronic granulomatous disease, autosomal 2, neutrophil NADPH oxidase factor 2, neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
Species: human
Products:     p67phox

Top Publications

  1. Gauss K, Bunger P, Larson T, Young C, Nelson Overton L, Siemsen D, et al. Identification of a novel tumor necrosis factor alpha-responsive region in the NCF2 promoter. J Leukoc Biol. 2005;77:267-78 pubmed
    ..assembly involves association of several membrane and cytosolic oxidase proteins, one of the cytosolic cofactors, p67phox, appears to play a more prominent role in final activation of the enzyme complex...
  2. Ammons M, Siemsen D, Nelson Overton L, Quinn M, Gauss K. Binding of pleomorphic adenoma gene-like 2 to the tumor necrosis factor (TNF)-alpha-responsive region of the NCF2 promoter regulates p67(phox) expression and NADPH oxidase activity. J Biol Chem. 2007;282:17941-52 pubmed
    ..These data clearly identify PLAGL2 as a novel regulator of NCF2 gene expression as well as NADPH oxidase activity and contribute to a greater understanding of the transcriptional regulation of NCF2. ..
  3. Dorseuil O, Gacon G. [Signal transduction by Rac small G proteins in phagocytes]. C R Seances Soc Biol Fil. 1997;191:237-46 pubmed
    ..enzyme, minimally requires assembly at the membrane with flavocytochrome b258 of cytosolic components p47phox, p67phox and Rac proteins...
  4. McAdara Berkowitz J, Catz S, Johnson J, Ruedi J, Thon V, Babior B. JFC1, a novel tandem C2 domain-containing protein associated with the leukocyte NADPH oxidase. J Biol Chem. 2001;276:18855-62 pubmed
    ..From the data presented here, it is proposed that JFC1 is acting as an adaptor protein between phosphatidylinositol 3-kinase products and the oxidase cytosolic complex. ..
  5. Takeshita F, Ishii K, Kobiyama K, Kojima Y, Coban C, Sasaki S, et al. TRAF4 acts as a silencer in TLR-mediated signaling through the association with TRAF6 and TRIF. Eur J Immunol. 2005;35:2477-85 pubmed
    ..These results suggest that TRAF4 participates in the molecular mechanism underlying silencing of TLR-mediated signaling through the interaction with molecules harboring phagosome/endosome membrane. ..
  6. Gauss K, Bunger P, Crawford M, McDermott B, Swearingen R, Nelson Overton L, et al. Variants of the 5'-untranslated region of human NCF2: expression and translational efficiency. Gene. 2006;366:169-79 pubmed
    ..These studies suggest that expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. ..
  7. Marty C, Kozasa T, Quinn M, Ye R. Activation state-dependent interaction between Galphai and p67phox. Mol Cell Biol. 2006;26:5190-200 pubmed
    ..We report here that the Galphai subunit of heterotrimeric G proteins is a novel binding partner for p67phox in transfected HEK293T cells and peripheral blood polymorphonuclear leukocytes...
  8. Leavey P, Gonzalez Aller C, Thurman G, Kleinberg M, Rinckel L, Ambruso D, et al. A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity. J Biol Chem. 2002;277:45181-7 pubmed
    ..Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H(2)O(2) or altering signaling pathways affected by H(2)O(2). ..
  9. Dang P, Cross A, Quinn M, Babior B. Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II. Proc Natl Acad Sci U S A. 2002;99:4262-5 pubmed
    Activation of the phagocyte NADPH oxidase complex requires assembly of the cytosolic factors p47PHOX, p67PHOX, p40PHOX, and Rac with the membrane-bound cytochrome b558...
  10. Thandavarayan R, Watanabe K, Ma M, Gurusamy N, Veeraveedu P, Konishi T, et al. Dominant-negative p38alpha mitogen-activated protein kinase prevents cardiac apoptosis and remodeling after streptozotocin-induced diabetes mellitus. Am J Physiol Heart Circ Physiol. 2009;297:H911-9 pubmed publisher
    ..In conclusion, our data establish that p38alpha MAPK activity is required for cardiac remodeling after diabetes induction and suggest that p38alpha MAPK may promote cardiomyocyte apoptosis by downregulation of Bcl-X(L). ..

Detail Information

Publications62

  1. Gauss K, Bunger P, Larson T, Young C, Nelson Overton L, Siemsen D, et al. Identification of a novel tumor necrosis factor alpha-responsive region in the NCF2 promoter. J Leukoc Biol. 2005;77:267-78 pubmed
    ..assembly involves association of several membrane and cytosolic oxidase proteins, one of the cytosolic cofactors, p67phox, appears to play a more prominent role in final activation of the enzyme complex...
  2. Ammons M, Siemsen D, Nelson Overton L, Quinn M, Gauss K. Binding of pleomorphic adenoma gene-like 2 to the tumor necrosis factor (TNF)-alpha-responsive region of the NCF2 promoter regulates p67(phox) expression and NADPH oxidase activity. J Biol Chem. 2007;282:17941-52 pubmed
    ..These data clearly identify PLAGL2 as a novel regulator of NCF2 gene expression as well as NADPH oxidase activity and contribute to a greater understanding of the transcriptional regulation of NCF2. ..
  3. Dorseuil O, Gacon G. [Signal transduction by Rac small G proteins in phagocytes]. C R Seances Soc Biol Fil. 1997;191:237-46 pubmed
    ..enzyme, minimally requires assembly at the membrane with flavocytochrome b258 of cytosolic components p47phox, p67phox and Rac proteins...
  4. McAdara Berkowitz J, Catz S, Johnson J, Ruedi J, Thon V, Babior B. JFC1, a novel tandem C2 domain-containing protein associated with the leukocyte NADPH oxidase. J Biol Chem. 2001;276:18855-62 pubmed
    ..From the data presented here, it is proposed that JFC1 is acting as an adaptor protein between phosphatidylinositol 3-kinase products and the oxidase cytosolic complex. ..
  5. Takeshita F, Ishii K, Kobiyama K, Kojima Y, Coban C, Sasaki S, et al. TRAF4 acts as a silencer in TLR-mediated signaling through the association with TRAF6 and TRIF. Eur J Immunol. 2005;35:2477-85 pubmed
    ..These results suggest that TRAF4 participates in the molecular mechanism underlying silencing of TLR-mediated signaling through the interaction with molecules harboring phagosome/endosome membrane. ..
  6. Gauss K, Bunger P, Crawford M, McDermott B, Swearingen R, Nelson Overton L, et al. Variants of the 5'-untranslated region of human NCF2: expression and translational efficiency. Gene. 2006;366:169-79 pubmed
    ..These studies suggest that expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. ..
  7. Marty C, Kozasa T, Quinn M, Ye R. Activation state-dependent interaction between Galphai and p67phox. Mol Cell Biol. 2006;26:5190-200 pubmed
    ..We report here that the Galphai subunit of heterotrimeric G proteins is a novel binding partner for p67phox in transfected HEK293T cells and peripheral blood polymorphonuclear leukocytes...
  8. Leavey P, Gonzalez Aller C, Thurman G, Kleinberg M, Rinckel L, Ambruso D, et al. A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity. J Biol Chem. 2002;277:45181-7 pubmed
    ..Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H(2)O(2) or altering signaling pathways affected by H(2)O(2). ..
  9. Dang P, Cross A, Quinn M, Babior B. Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II. Proc Natl Acad Sci U S A. 2002;99:4262-5 pubmed
    Activation of the phagocyte NADPH oxidase complex requires assembly of the cytosolic factors p47PHOX, p67PHOX, p40PHOX, and Rac with the membrane-bound cytochrome b558...
  10. Thandavarayan R, Watanabe K, Ma M, Gurusamy N, Veeraveedu P, Konishi T, et al. Dominant-negative p38alpha mitogen-activated protein kinase prevents cardiac apoptosis and remodeling after streptozotocin-induced diabetes mellitus. Am J Physiol Heart Circ Physiol. 2009;297:H911-9 pubmed publisher
    ..In conclusion, our data establish that p38alpha MAPK activity is required for cardiac remodeling after diabetes induction and suggest that p38alpha MAPK may promote cardiomyocyte apoptosis by downregulation of Bcl-X(L). ..
  11. Dang P, Cross A, Babior B. Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558. Proc Natl Acad Sci U S A. 2001;98:3001-5 pubmed
    ..Rac1-GTP or Rac1-GDP increased the binding of p67(PHOX) to cytochrome b(558), suggesting that at least one of the oxidase-related functions of Rac1 is to promote the interaction between p67(PHOX) and cytochrome b(558). ..
  12. Rush J, Laughlin M, Woodman C, Price E. SOD-1 expression in pig coronary arterioles is increased by exercise training. Am J Physiol Heart Circ Physiol. 2000;279:H2068-76 pubmed
    ..dependent dilation previously observed in EX porcine coronary arterioles by improving management of superoxide in the vascular cell environment, thus prolonging the biological half-life of NO...
  13. Wang X, Son Y, Chang Q, Sun L, Hitron J, Budhraja A, et al. NADPH oxidase activation is required in reactive oxygen species generation and cell transformation induced by hexavalent chromium. Toxicol Sci. 2011;123:399-410 pubmed publisher
    ..Our results suggest that NOX plays an important role in Cr(VI)-induced ROS generation and carcinogenesis...
  14. Grandvaux N, Grizot S, Vignais P, Dagher M. The Ku70 autoantigen interacts with p40phox in B lymphocytes. J Cell Sci. 1999;112 ( Pt 4):503-13 pubmed
    ..We also show that the two NADPH oxidase activating factors, p47phox and p67phox are substrates for DNA-PK in vitro and that they are present together with p40phox in the nucleus of B cells...
  15. Woolery A, Yu X, Labaer J, Orth K. AMPylation of Rho GTPases subverts multiple host signaling processes. J Biol Chem. 2014;289:32977-88 pubmed publisher
    ..We conclude that AMPylation of Rho Family GTPases by VopS results in diverse inhibitory consequences during infection beyond the most obvious phenotype, the collapse of the actin cytoskeleton. ..
  16. Dahan I, Smith S, Pick E. A Cys-Gly-Cys triad in the dehydrogenase region of Nox2 plays a key role in the interaction with p67phox. J Leukoc Biol. 2015;98:859-74 pubmed publisher
    ..The 2 regions interact with distinct partner sites in p67(phox). ..
  17. Singh A, Singh V, Tiwari R, Chandra T, Kumar A, Dikshit M, et al. The IRAK-ERK-p67phox-Nox-2 axis mediates TLR4, 2-induced ROS production for IL-1β transcription and processing in monocytes. Cell Mol Immunol. 2016;13:745-763 pubmed publisher
    ..the role of interleukin-1 receptor-associated kinase (IRAK), extracellular signal-regulated kinase (ERK), p67phox and Nox-2 in TLR4- and TLR2-induced ROS generation during interleukin-1 beta (IL-1β) transcription, processing, ..
  18. Kenney R, Leto T. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 1990;18:7193 pubmed
  19. Yu B, Chen Y, Wu Q, Li P, Shao Y, Zhang J, et al. The association between single-nucleotide polymorphisms of NCF2 and systemic lupus erythematosus in Chinese mainland population. Clin Rheumatol. 2011;30:521-7 pubmed publisher
    ..17-1.95) and anti-DNA (p = 0.013, OR = 1.40, and 95%CI = 1.07-1.82). Polymorphisms of rs13277113 in NCF2 gene were associated with arthritis and autoantibody production, but not disease risk, of SLE in Chinese population. ..
  20. Bhatti P, Stewart P, Hutchinson A, Rothman N, Linet M, Inskip P, et al. Lead exposure, polymorphisms in genes related to oxidative stress, and risk of adult brain tumors. Cancer Epidemiol Biomarkers Prev. 2009;18:1841-8 pubmed publisher
    ..Although the results of this study provide some evidence that lead may cause glioblastoma multiforme and meningioma through mechanisms related to oxidative damage, the results must be confirmed in other populations. ..
  21. Kannengiesser C, Gerard B, El Benna J, Henri D, Kroviarski Y, Chollet Martin S, et al. Molecular epidemiology of chronic granulomatous disease in a series of 80 kindreds: identification of 31 novel mutations. Hum Mutat. 2008;29:E132-49 pubmed publisher
    ..We observed that significantly fewer females were affected by autosomal recessive CGD than expected (2 females/14 males; p=0.002), suggesting that female patients with CGD may be under diagnosed. ..
  22. Bissonnette S, Glazier C, Stewart M, Brown G, Ellson C, Yaffe M. Phosphatidylinositol 3-phosphate-dependent and -independent functions of p40phox in activation of the neutrophil NADPH oxidase. J Biol Chem. 2008;283:2108-19 pubmed
    ..We present a model where p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement. ..
  23. Tlili A, Erard M, Faure M, Baudin X, Piolot T, Dupre Crochet S, et al. Stable accumulation of p67phox at the phagosomal membrane and ROS production within the phagosome. J Leukoc Biol. 2012;91:83-95 pubmed publisher
    ..These data support the critical role of p67(phox) for ROS production on the level of individual phagosomes. ..
  24. Gómez L, Patiño P, Novaira H, Morales M, Condino Neto A. A single nucleotide polymorphism in the promoter region of theNCF-2 gene. Am J Hematol. 2007;82:1124-5 pubmed
  25. La Favor J, Dubis G, Yan H, White J, Nelson M, Anderson E, et al. Microvascular Endothelial Dysfunction in Sedentary, Obese Humans Is Mediated by NADPH Oxidase: Influence of Exercise Training. Arterioscler Thromb Vasc Biol. 2016;36:2412-2420 pubmed
    ..Furthermore, aerobic exercise training proved to be an effective strategy for alleviating these maladies. ..
  26. Miller T, Lee D, Giefer M, Wahbeh G, Suskind D. Nutritional Therapy in Very Early-Onset Inflammatory Bowel Disease: A Case Report. Dig Dis Sci. 2017;62:2196-2200 pubmed publisher
    ..We describe two cases in which infants presenting with VEO-IBD achieved clinical remission using exclusive enteral nutrition, a formula-based diet which has been shown to induce remission in older children with active Crohn's disease. ..
  27. Liu H, Liu J, Li H, Peng Y, Zhao S. Mimicking hypersensitivity pneumonitis as an uncommon initial presentation of chronic granulomatous disease in children. Orphanet J Rare Dis. 2017;12:169 pubmed publisher
    ..A prompt diagnosis of CGD is essential to enable initiation of prophylactic antibacterial and antifungal therapies. ..
  28. Tamura M, Kai T, Tsunawaki S, Lambeth J, Kameda K. Direct interaction of actin with p47(phox) of neutrophil NADPH oxidase. Biochem Biophys Res Commun. 2000;276:1186-90 pubmed
    ..Far-Western blotting using cytosol as a target showed an interaction between actin and endogenous p47(phox) and rac proteins. These results suggest that actin can directly interact with p47(phox) and possibly with rac in the cells. ..
  29. Leto T, Lomax K, Volpp B, Nunoi H, Sechler J, Nauseef W, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src. Science. 1990;248:727-30 pubmed
    ....
  30. Ito T, Matsui Y, Ago T, Ota K, Sumimoto H. Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions. EMBO J. 2001;20:3938-46 pubmed
    ..This domain-swapping experiment demonstrates that Bem1p function requires interaction with Cdc24p, in which the PB1 domain and the PC motif participate as responsible modules. ..
  31. Gauss K, Bunger P, Quinn M. AP-1 is essential for p67(phox) promoter activity. J Leukoc Biol. 2002;71:163-72 pubmed
    ..In support of this conclusion, mutagenesis of all three sites completely abrogated transcriptional activity. ..
  32. Gorzalczany Y, Alloul N, Sigal N, Weinbaum C, Pick E. A prenylated p67phox-Rac1 chimera elicits NADPH-dependent superoxide production by phagocyte membranes in the absence of an activator and of p47phox: conversion of a pagan NADPH oxidase to monotheism. J Biol Chem. 2002;277:18605-10 pubmed
    ....
  33. Zhao X, Xu B, Bhattacharjee A, Oldfield C, Wientjes F, Feldman G, et al. Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. J Leukoc Biol. 2005;77:414-20 pubmed
    Phosphorylation of the reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase components p67phox and p47phox accompanies the assembly and activation of this enzyme complex...
  34. Menden H, Xia S, Mabry S, Navarro A, Nyp M, Sampath V. Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 Regulates LPS-Induced Inflammation and Alveolar Remodeling in the Developing Lung. Am J Respir Cell Mol Biol. 2016;55:767-778 pubmed
    ..LPS-induced NOX2 assembly evident by p67phox/gp91phox coimmunoprecipitation was disrupted with NOX2-I...
  35. Takeya R, Ueno N, Kami K, Taura M, Kohjima M, Izaki T, et al. Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases. J Biol Chem. 2003;278:25234-46 pubmed
    ..The cytochrome becomes activated by interacting with the adaptor proteins p47phox and p67phox as well as the small GTPase Rac...
  36. Cunninghame Graham D, Morris D, Bhangale T, Criswell L, Syvanen A, Rönnblom L, et al. Association of NCF2, IKZF1, IRF8, IFIH1, and TYK2 with systemic lupus erythematosus. PLoS Genet. 2011;7:e1002341 pubmed publisher
    ..These results increase the number of established susceptibility genes for lupus to ~30 and validate the importance of using large datasets to confirm associations of loci which moderately increase the risk for disease. ..
  37. DeLeo F, Quinn M. Assembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteins. J Leukoc Biol. 1996;60:677-91 pubmed
    ....
  38. Kerkhoff C, Nacken W, Benedyk M, Dagher M, Sopalla C, Doussiere J. The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2. FASEB J. 2005;19:467-9 pubmed
    ..revealed that S100A8 is the privileged interaction partner for the NADPH oxidase complex since it bound to p67phox and Rac, whereas S100A9 did interact with neither p67phox nor p47phox...
  39. Lomnytska M, Becker S, Bodin I, Olsson A, Hellman K, Hellström A, et al. Differential expression of ANXA6, HSP27, PRDX2, NCF2, and TPM4 during uterine cervix carcinogenesis: diagnostic and prognostic value. Br J Cancer. 2011;104:110-9 pubmed publisher
    ..Detection of expression changes of the proteins ANXA6, HSP27, PRDX2, NCF2, and TPM4 in SCC precursor lesions may aid current cytological and pathological diagnostics and evaluation of prognosis. ..
  40. Cheng G, Ritsick D, Lambeth J. Nox3 regulation by NOXO1, p47phox, and p67phox. J Biol Chem. 2004;279:34250-5 pubmed
    ..The unique regulation of Nox3 supports a model in which multiple interactions with regulatory subunits stabilize an active conformation of the catalytic subunit. ..
  41. Hord J, Botchlett R, Lawler J. Age-related alterations in the sarcolemmal environment are attenuated by lifelong caloric restriction and voluntary exercise. Exp Gerontol. 2016;83:148-57 pubmed publisher
    ..OAL fibers also displayed reduced protein content of NADPH oxidase isoform 2 (Nox2) subunits (p67phox and p47phox), consistent with a perturbed sarcolemmal environment...
  42. Lee J, Park A, Lee K, Park S, Han S, Han W, et al. Candidate gene approach evaluates association between innate immunity genes and breast cancer risk in Korean women. Carcinogenesis. 2009;30:1528-31 pubmed publisher
    ..004, respectively). This study suggests that common genetic variants in the OR10J3 and FCER1A be strongly associated with breast cancer risk among Korean women. ..
  43. Li X, Tian W, Stull N, Grinstein S, Atkinson S, Dinauer M. A fluorescently tagged C-terminal fragment of p47phox detects NADPH oxidase dynamics during phagocytosis. Mol Biol Cell. 2009;20:1520-32 pubmed publisher
    ..This study defines a sequence of events during phagocytosis-induced NADPH oxidase assembly and provides experimental evidence that intermolecular interactions within this complex are dynamic and modulated after assembly on phagosomes. ..
  44. Roberts J, Atanasova K, Lee J, Diamond G, Deguzman J, Hee Choi C, et al. Opportunistic Pathogen Porphyromonas gingivalis Modulates Danger Signal ATP-Mediated Antibacterial NOX2 Pathways in Primary Epithelial Cells. Front Cell Infect Microbiol. 2017;7:291 pubmed publisher
    ..temporally modulate NOX2 pathway by reorganizing the localization and activation of cytosolic molecules (p47phox, p67phox, and Rac1) during 24 h of infection...
  45. Song Z, Bouchab L, Hudik E, Le Bars R, Nüsse O, Dupre Crochet S. Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome. J Leukoc Biol. 2017;101:1155-1168 pubmed publisher
    ..In conclusion, PI(3)P, indeed, regulates ROS production by maintaining p40phox and p67phox at the phagosomal membrane. ..
  46. El Hawary R, Meshaal S, Deswarte C, Galal N, Abdelkawy M, Alkady R, et al. Role of Flow Cytometry in the Diagnosis of Chronic Granulomatous Disease: the Egyptian Experience. J Clin Immunol. 2016;36:610-8 pubmed publisher
    ..The identification of defective proteins aids in establishing a diagnosis prior to genetic analysis, which is rather labor-intensive, expensive, and time-consuming...
  47. Leto T, Adams A, de Mendez I. Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets. Proc Natl Acad Sci U S A. 1994;91:10650-4 pubmed
    ..We propose a model in which the oxidase complex assembles through a mechanism involving SH3 domains of both cytosolic proteins and cognate proline-rich targets in other oxidase components. ..
  48. Vignesh P, Rawat A, Kumar A, Suri D, Gupta A, Lau Y, et al. Chronic Granulomatous Disease Due to Neutrophil Cytosolic Factor (NCF2) Gene Mutations in Three Unrelated Families. J Clin Immunol. 2017;37:109-112 pubmed publisher
    ..The mother of this child was a carrier for the IVS13-2A>T mutation. All three cases had colitis, and it was the initial symptom in two patients. One of the patients also developed a lung abscess due to Nocardia cyriacigeorgica. ..
  49. Bonizzato A, Russo M, Donini M, Dusi S. Identification of a double mutation (D160V-K161E) in the p67phox gene of a chronic granulomatous disease patient. Biochem Biophys Res Commun. 1997;231:861-3 pubmed
    In neutrophils of a chronic granulomatous disease (CGD) patient with a lack of p67phox the mRNA for p67phox was present in normal amount and size...
  50. Sathyamoorthy M, de Mendez I, Adams A, Leto T. p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain. J Biol Chem. 1997;272:9141-6 pubmed
    ..Thus, p40(phox) appears to down-regulate oxidase function by competing with an SH3 domain interaction between other essential oxidase components. ..
  51. Badalzadeh M, Fattahi F, Fazlollahi M, Tajik S, Bemanian M, Behmanesh F, et al. Molecular analysis of four cases of chronic granulomatous disease caused by defects in NCF-2: the gene encoding the p67-phox. Iran J Allergy Asthma Immunol. 2012;11:340-4 pubmed publisher
    ..Although a gross deletion in other exons of NCF2 has been previously reported, a large deletion encompassing exon 2 has been not reported yet. This abstract was also presented in ESID 2012, Florence, Italy. ..
  52. Faris S, Rinckel L, Huang J, Hong Y, Kleinberg M. Phagocyte NADPH oxidase p67-phox possesses a novel carboxylterminal binding site for the GTPases Rac2 and Cdc42. Biochem Biophys Res Commun. 1998;247:271-6 pubmed
    ..Therefore, Rac binding to p67-phox is not synonymous with NADPH oxidase activation, and Rac probably participates in other steps of oxidase activation in addition to binding p67-phox. ..
  53. Guzik T, Sadowski J, Kapelak B, Jopek A, Rudzinski P, Pillai R, et al. Systemic regulation of vascular NAD(P)H oxidase activity and nox isoform expression in human arteries and veins. Arterioscler Thromb Vasc Biol. 2004;24:1614-20 pubmed
    ..However, molecular mechanisms regulating superoxide production in human blood vessels remain poorly defined...
  54. Yuzawa S, Miyano K, Honbou K, Inagaki F, Sumimoto H. The domain organization of p67 phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. J Innate Immun. 2009;1:543-55 pubmed publisher
    ....
  55. Lapouge K, Smith S, Groemping Y, Rittinger K. Architecture of the p40-p47-p67phox complex in the resting state of the NADPH oxidase. A central role for p67phox. J Biol Chem. 2002;277:10121-8 pubmed
    ..These findings, in combination with earlier reports, allow us to propose a model for the architecture of the resting complex in which p67(phox) acts as the bridging molecule that connects p40(phox) and p47(phox). ..
  56. San José G, Bidegain J, Robador P, Diez J, Fortuno A, Zalba G. Insulin-induced NADPH oxidase activation promotes proliferation and matrix metalloproteinase activation in monocytes/macrophages. Free Radic Biol Med. 2009;46:1058-67 pubmed publisher
    ....
  57. Shmelzer Z, Karter M, Eisenstein M, Leto T, Hadad N, Ben Menahem D, et al. Cytosolic phospholipase A2alpha is targeted to the p47phox-PX domain of the assembled NADPH oxidase via a novel binding site in its C2 domain. J Biol Chem. 2008;283:31898-908 pubmed publisher
    ..energy transfer analysis and studies in neutrophils from patients with chronic granulomatous disease deficient in p67phox or p47phox, that cPLA2 specifically binds to p47phox and that p47phox is sufficient to anchor cPLA2 to the ..
  58. Martel C, Mollin M, Beaumel S, Brion J, Coutton C, Satre V, et al. Clinical, functional and genetic analysis of twenty-four patients with chronic granulomatous disease - identification of eight novel mutations in CYBB and NCF2 genes. J Clin Immunol. 2012;32:942-58 pubmed publisher
    ..No clear correlation between clinical severity and CYBB mutations could be established. Of three mutations in CYBA, NCF1 and NCF2 leading to rare autosomal recessive CGD, one nonsense mutation c29G>A in exon 1 of NCF2 was new. ..
  59. Francke U, Hsieh C, Foellmer B, Lomax K, Malech H, Leto T. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am J Hum Genet. 1990;47:483-92 pubmed
    ..of CGD result from defects in cytosolic components of the phagocyte NADPH oxidase system, p47phox (NCF1) and p67phox (NCF2). By using human cDNA probes we have mapped the genes for these proteins to chromosomal sites...
  60. Li J, Shah A. Intracellular localization and preassembly of the NADPH oxidase complex in cultured endothelial cells. J Biol Chem. 2002;277:19952-60 pubmed
    ..These results indicate that, in contrast to the neutrophil enzyme, a substantial proportion of the NADPH oxidase in unstimulated endothelial cells exists as a preassembled intracellular complex associated with the cytoskeleton. ..
  61. Gauss K, Nelson Overton L, Siemsen D, Gao Y, Deleo F, Quinn M. Role of NF-kappaB in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-alpha. J Leukoc Biol. 2007;82:729-41 pubmed
    ....