Sp1

Summary

Gene Symbol: Sp1
Description: Sp1 transcription factor
Alias: transcription factor Sp1, specificity protein 1
Species: human

Top Publications

  1. doi FoxO1 inhibits leptin regulation of pro-opiomelanocortin promoter activity by blocking STAT3 interaction with specificity protein 1
    Guoqing Yang
    Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667
    J Biol Chem 284:3719-27. 2009
  2. pmc MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1
    Y Xu
    Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang City, China
    Oncogene 31:1398-407. 2012
  3. ncbi The tumor suppressor interferon regulatory factor 1 interferes with SP1 activation to repress the human CDK2 promoter
    Rong Lin Xie
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 278:26589-96. 2003
  4. pmc Curcumin decreases specificity protein expression in bladder cancer cells
    Gayathri Chadalapaka
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843 4466, USA
    Cancer Res 68:5345-54. 2008
  5. doi Dysregulation of p53/Sp1 control leads to DNA methyltransferase-1 overexpression in lung cancer
    Ruo Kai Lin
    Department of Pharmacology, College of Medicine, and Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan, ROC
    Cancer Res 70:5807-17. 2010
  6. pmc The p65 subunit of NF-κB inhibits COL1A1 gene transcription in human dermal and scleroderma fibroblasts through its recruitment on promoter by protein interaction with transcriptional activators (c-Krox, Sp1, and Sp3)
    Gallic Beauchef
    Laboratoire Matrice Extracellulaire et Pathologie, IFR ICORE 146, Universite de Caen Basse Normandie, UFR de Medecine, CHU niveau 3, Avenue de la Cote de Nacre, 14032 Caen Cedex, France
    J Biol Chem 287:3462-78. 2012
  7. doi Overexpression of Sp1 leads to p53-dependent apoptosis in cancer cells
    Jian Ying Chuang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Int J Cancer 125:2066-76. 2009
  8. pmc Sp1 regulates chromatin looping between an intronic enhancer and distal promoter of the human heme oxygenase-1 gene in renal cells
    Jessy Deshane
    Department of Medicine, Nephrology Research and Training Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 285:16476-86. 2010
  9. pmc Leptin increases tissue inhibitor of metalloproteinase I (TIMP-1) gene expression by a specificity protein 1/signal transducer and activator of transcription 3 mechanism
    Songbai Lin
    Department of Medicine, Division of Digestive Diseases, Emory University School of Medicine, Room 248, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, Georgia 30322, USA
    Mol Endocrinol 20:3376-88. 2006
  10. pmc Sp1/NFkappaB/HDAC/miR-29b regulatory network in KIT-driven myeloid leukemia
    Shujun Liu
    Division of Hematology Oncology, The Ohio State University, Columbus, OH 43210, USA
    Cancer Cell 17:333-47. 2010

Research Grants

  1. Melissa Churchill; Fiscal Year: 2014
  2. Enhance Production of Functional Recombinant Human Protein C Variant In Mammalian
    Nienwen Chow; Fiscal Year: 2010
  3. Ashok Kumar; Fiscal Year: 2016
  4. Jean Pierre J Issa; Fiscal Year: 2016
  5. Dietary HDAC Inhibitors in Colon Cancer Prevention
    Roderick H Dashwood; Fiscal Year: 2012
  6. Transcriptional regulation of enteroendocrine cell differentiation by NeuroD
    Andrew B Leiter; Fiscal Year: 2013
  7. Susana T Valente; Fiscal Year: 2016
  8. Carole R Mendelson; Fiscal Year: 2016
  9. SPORE in Pancreatic Cancer
    James Abbruzzese; Fiscal Year: 2009
  10. Sp1, kappa-B enhancers and transcriptions in neurons
    STEVEN BARGER; Fiscal Year: 2009

Detail Information

Publications312 found, 100 shown here

  1. doi FoxO1 inhibits leptin regulation of pro-opiomelanocortin promoter activity by blocking STAT3 interaction with specificity protein 1
    Guoqing Yang
    Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667
    J Biol Chem 284:3719-27. 2009
    ..that phospho-STAT3 activates POMC promoter in response to leptin signaling through a mechanism that requires an SP1-binding site in the POMC promoter...
  2. pmc MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1
    Y Xu
    Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang City, China
    Oncogene 31:1398-407. 2012
    ..Furthermore, miR-335 might suppress gastric cancer invasion and metastasis by targeting Bcl-w and specificity protein 1 (SP1)...
  3. ncbi The tumor suppressor interferon regulatory factor 1 interferes with SP1 activation to repress the human CDK2 promoter
    Rong Lin Xie
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 278:26589-96. 2003
    ..The -68/-31 segment lacks a canonical IRF responsive element but contains a single SP1 binding site...
  4. pmc Curcumin decreases specificity protein expression in bladder cancer cells
    Gayathri Chadalapaka
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843 4466, USA
    Cancer Res 68:5345-54. 2008
    ..The results show that curcumin induced proteasome-dependent down-regulation of Sp1, Sp3, and Sp4 in 253JB-V and KU7 cells...
  5. doi Dysregulation of p53/Sp1 control leads to DNA methyltransferase-1 overexpression in lung cancer
    Ruo Kai Lin
    Department of Pharmacology, College of Medicine, and Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan, ROC
    Cancer Res 70:5807-17. 2010
    ..Here, we showed that wild-type p53 negatively regulated DNMT1 expression by forming a complex with specificity protein 1 (Sp1) protein and chromatin modifiers on the DNMT1 promoter...
  6. pmc The p65 subunit of NF-κB inhibits COL1A1 gene transcription in human dermal and scleroderma fibroblasts through its recruitment on promoter by protein interaction with transcriptional activators (c-Krox, Sp1, and Sp3)
    Gallic Beauchef
    Laboratoire Matrice Extracellulaire et Pathologie, IFR ICORE 146, Universite de Caen Basse Normandie, UFR de Medecine, CHU niveau 3, Avenue de la Cote de Nacre, 14032 Caen Cedex, France
    J Biol Chem 287:3462-78. 2012
    ..This 51-bp region mediates the action of two zinc fingers, Sp1 (specific protein-1) and Sp3, acting as trans-activators of type I collagen expression in ANF and SF...
  7. doi Overexpression of Sp1 leads to p53-dependent apoptosis in cancer cells
    Jian Ying Chuang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Int J Cancer 125:2066-76. 2009
    Numerous studies have documented that Sp1 expression level were elevated in various human cancers...
  8. pmc Sp1 regulates chromatin looping between an intronic enhancer and distal promoter of the human heme oxygenase-1 gene in renal cells
    Jessy Deshane
    Department of Medicine, Nephrology Research and Training Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 285:16476-86. 2010
    ..site-directed mutagenesis, and chromatin immunoprecipitation assays confirmed the functional interaction of Sp1 with a consensus binding sequence within the 220-bp region. Mutations of regulatory elements within the -4...
  9. pmc Leptin increases tissue inhibitor of metalloproteinase I (TIMP-1) gene expression by a specificity protein 1/signal transducer and activator of transcription 3 mechanism
    Songbai Lin
    Department of Medicine, Division of Digestive Diseases, Emory University School of Medicine, Room 248, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, Georgia 30322, USA
    Mol Endocrinol 20:3376-88. 2006
    ..An EMSA revealed that leptin enhanced specificity protein 1 (Sp1) binding...
  10. pmc Sp1/NFkappaB/HDAC/miR-29b regulatory network in KIT-driven myeloid leukemia
    Shujun Liu
    Division of Hematology Oncology, The Ohio State University, Columbus, OH 43210, USA
    Cancer Cell 17:333-47. 2010
    ..we show that KIT mutations lead to MYC-dependent miR-29b repression and increased levels of the miR-29b target Sp1 in KIT-driven leukemia...
  11. ncbi Oxidative stress regulates vascular endothelial growth factor-A gene transcription through Sp1- and Sp3-dependent activation of two proximal GC-rich promoter elements
    Georgia Schäfer
    Medizinische Klinik mit Schwerpunkt Hepatologie, Gastroenterologie, Endokrinologie und Stoffwechsel, Charite, Campus Virchow Klinikum, 13353 Berlin, Germany
    J Biol Chem 278:8190-8. 2003
    ..Combination of EMSA studies, Sp1/Sp3 overexpression experiments in Drosophila SL-2 cells, and systematic promoter mutagenesis identified enhanced ..
  12. pmc Sp1 and AP-1 regulate expression of the human gene VIL2 in esophageal carcinoma cells
    Shu Ying Gao
    Department of Biochemistry and Molecular Biology, Shantou University, Shantou, China
    J Biol Chem 284:7995-8004. 2009
    ..We found that the region -87/-32 containing consensus Sp1 (-75/-69) and AP-1 (-64/-58) binding sites is crucial for VIL2 promoter activity in esophageal carcinoma cells (..
  13. ncbi A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans
    Gareth L Bond
    Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08903, USA
    Cell 119:591-602. 2004
    ..SNP309) is found in the MDM2 promoter and is shown to increase the affinity of the transcriptional activator Sp1, resulting in higher levels of MDM2 RNA and protein and the subsequent attenuation of the p53 pathway...
  14. doi Transcription factor Sp1 regulates expression of cancer-associated molecule CD147 in human lung cancer
    Ling min Kong
    Department of Cell Biology, Fourth Military Medical University, Xi an, China
    Cancer Sci 101:1463-70. 2010
    ..the human CD147 gene and identified a critical promoter region at -108 to -42 which contained one binding site for Sp1, which was essential in up-regulating CD147 promoter activity...
  15. ncbi IL-10 regulation by HIV-Tat in primary human monocytic cells: involvement of calmodulin/calmodulin-dependent protein kinase-activated p38 MAPK and Sp-1 and CREB-1 transcription factors
    Katrina Gee
    Department of Pathology and Laboratory Medicine, Division of Virology and Molecular Immunology, Children s Hospital of Eastern Ontario, 401 Smyth Road, Ottawa, Ontario, Canada
    J Immunol 178:798-807. 2007
    ..Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II-dependent p38 MAPK...
  16. ncbi Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter
    H Rotheneder
    Vienna Biocenter, University of Vienna, Austria
    J Mol Biol 293:1005-15. 1999
    ..of growth and cell cycle regulated genes frequently carry binding sites for transcription factors of the E2F and Sp1 families...
  17. pmc Central role of Sp1-regulated CD39 in hypoxia/ischemia protection
    Holger K Eltzschig
    Department of Anesthesiology and Perioperative Medicine, University of Colorado Health Sciences Center, Denver, CO, USA
    Blood 113:224-32. 2009
    ..chromatin immunoprecipitation, and inhibition by antisense, revealed a critical role for transcription-factor Sp1 in hypoxia-induction of CD39...
  18. pmc Bortezomib induces DNA hypomethylation and silenced gene transcription by interfering with Sp1/NF-kappaB-dependent DNA methyltransferase activity in acute myeloid leukemia
    Shujun Liu
    Division of Hematology Oncology, The Ohio State University, Columbus, OH 43210, USA
    Blood 111:2364-73. 2008
    ..Zinc finger protein Sp1 transactivates DNMT1 gene in mice and is functionally regulated through protein abundance, posttranslational ..
  19. ncbi Synergistic activation by the glutamine-rich domains of human transcription factor Sp1
    A J Courey
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Cell 59:827-36. 1989
    We have examined the role of protein-protein interactions in modulating the activity of Sp1, a human transcription factor that utilizes glutamine-rich activation domains...
  20. ncbi Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro
    N Mermod
    Department of Biochemistry, University of California, Berkeley 94720
    Nature 332:557-61. 1988
    ..Thus by manipulating the levels of different trans-acting factors in vitro, we can recreate the pattern of SV40 late initiation observed during the viral lytic cycle in vivo...
  21. pmc Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon
    M R López-Huertas
    AIDS Immunopathology Unit, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain
    Nucleic Acids Res 38:3287-307. 2010
    ..Functional experiments were performed to evaluate the effect of this deregulation. First, NF-kappaB-, NF-AT- and Sp1-dependent transcriptional activities were greatly enhanced in Jurkat-Tat101, whereas Tat72 induced milder but ..
  22. doi Sp1 is a transcription repressor to stanniocalcin-1 expression in TSA-treated human colon cancer cells, HT29
    Alice Y S Law
    Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong
    J Cell Biochem 112:2089-96. 2011
    ..In this study, we reported that the transcriptional factor, specificity protein 1 (Sp1) in association with retinoblastoma (Rb) repressed STC1 gene transcription in TSA-treated HT29 cells...
  23. ncbi Co-stimulation of promoter for low density lipoprotein receptor gene by sterol regulatory element-binding protein and Sp1 is specifically disrupted by the yin yang 1 protein
    M K Bennett
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 274:13025-32. 1999
    ..element-binding proteins (SREBPs) and promoter-specific but generic co-regulatory transcription factors such as Sp1 and NF-Y/CBF...
  24. pmc Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1
    Joanna Wysocka
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Genes Dev 17:896-911. 2003
    ..These results suggest that HCF-1 can broadly regulate transcription, both positively and negatively, through selective modulation of chromatin structure...
  25. ncbi ICI182,780 induces p21Waf1 gene transcription through releasing histone deacetylase 1 and estrogen receptor alpha from Sp1 sites to induce cell cycle arrest in MCF-7 breast cancer cell line
    Rana Varshochi
    Cancer Research UK Laboratories and Section of Cancer Cell Biology, Department of Cancer Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 ONN, United Kingdom
    J Biol Chem 280:3185-96. 2005
    ..the induction of p21Waf1 by ICI182,780 is mediated at transcriptional and gene promoter levels through the proximal Sp1 sites located near the transcription start site...
  26. pmc Sp1 expression is disrupted in schizophrenia; a possible mechanism for the abnormal expression of mitochondrial complex I genes, NDUFV1 and NDUFV2
    Dorit Ben-Shachar
    Laboratory of Psychobiology, Department of Psychiatry, Rambam Medical Center, Ruth and Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    PLoS ONE 2:e817. 2007
    ..We investigated whether the ubiquitous transcription factor Sp1 is abnormally expressed in schizophrenia, and consequently can affect the expression of genes implicated ..
  27. doi Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1
    Tatsuo Ito
    Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1 78 Sho machi, Tokushima 770 8505, Japan
    Biochem Biophys Res Commun 403:161-6. 2010
    b>Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood...
  28. pmc Coactivator function of positive cofactor 4 (PC4) in Sp1-directed luteinizing hormone receptor (LHR) gene transcription
    Mingjuan Liao
    Molecular Endocrinology Section, Program of Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 286:7681-91. 2011
    ..While overexpression of PC4 alone had no effect on the LHR gene, it significantly enhanced Sp1- but not Sp3-mediated LHR transcriptional activity...
  29. pmc HIF-1α depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs
    Carolyn Culver
    College of Life Sciences, Wellcome Trust Centre for Gene Regulation and Expression, MSI WTB JBC Complex, University of Dundee, Dundee, Scotland, UK
    Cell Cycle 10:1249-60. 2011
    ..p21 is induced via, at least in part, p53-independent but SP1-dependent mechanisms. Interestingly, HIF-1α knockdown also alters the cellular response to chemotherapeutic agents...
  30. ncbi Galectin-3 enhances cyclin D(1) promoter activity through SP1 and a cAMP-responsive element in human breast epithelial cells
    Huei Min Lin
    Department of Pathology, Wayne State University, School of Medicine, Detroit, Michigan, MI 48201, USA
    Oncogene 21:8001-10. 2002
    ..activity in human breast epithelial cells independent of cell adhesion through multiple cis-elements, including the SP1 and CRE sites...
  31. pmc Regulation of the human SOX9 promoter by Sp1 and CREB
    Sonsoles Piera-Velazquez
    Department of Medicine, Division of Rheumatology, Thomas Jefferson University, Jefferson Medical College, 233 S 10th Street, Room 509 BLSB, Philadelphia, PA 19107 5541, USA
    Exp Cell Res 313:1069-79. 2007
    ..the human SOX9 proximal promoter is also regulated by the cyclic-AMP response element binding protein (CREB) and Sp1. We show by DNaseI protection and EMSA analysis that CREB and Sp1 interact with specific sites within the SOX9 ..
  32. pmc Transforming growth factor-beta1 up-regulation of human alpha(1)(I) collagen is mediated by Sp1 and Smad2 transacting factors
    Polina Sysa
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    DNA Cell Biol 28:425-34. 2009
    ..Smads bind in association with Sp1 to the CC(GG)-rich TGFbeta1 responsive element of the promoter that lacks the classical Smad recognition element, ..
  33. pmc Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression [corrected] of transcription of the luteinizing hormone receptor gene
    Ying Zhang
    Section on Molecular Endocrinology, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development NIH, 49 Convent Drive MSC 4510, Bethesda, MD 20892 4510, USA
    Mol Cell Biol 26:6748-61. 2006
    ..demonstrated that silencing of luteinizing hormone receptor (LHR) gene transcription is mediated via a proximal Sp1 site at its promoter...
  34. pmc Role of SP1-binding domains in in vivo transcriptional regulation of the human immunodeficiency virus type 1 long terminal repeat
    D Harrich
    Department of Medicine, School of Medicine, University of California, Los Angeles 90024
    J Virol 63:2585-91. 1989
    ..These include the negative regulatory, enhancer, SP1, TATA, and TAR regions...
  35. ncbi A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2
    H Ge
    Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 2:751-9. 1998
    ..Together, these observations suggest that, in addition to functioning as a transcriptional coactivator, p52 may also act as an adaptor to coordinate pre-mRNA splicing and transcriptional activation of class II genes...
  36. ncbi Cloning and characterization of the 5'-flanking region of the human transcription factor Sp1 gene
    M Nicolas
    Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain
    J Biol Chem 276:22126-32. 2001
    The 5'-flanking region of the human Sp1 gene was cloned and characterized. Sequence analysis of this region showed the absence of both CAAT and TATA boxes and an initiator element...
  37. ncbi 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression
    Chibo Hong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Carcinogenesis 23:1297-305. 2002
    ..the first 291 bp segment of the proximal promoter, which contains six promoter specific transcription factor 1 (Sp1) elements, maintained DIM responsiveness...
  38. ncbi HIF-1alpha induces genetic instability by transcriptionally downregulating MutSalpha expression
    Minori Koshiji
    Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 17:793-803. 2005
    ..HIF-1alpha displaces the transcriptional activator Myc from Sp1 binding to repress MutSalpha expression in a p53-dependent manner; Sp1 serves as a molecular switch by recruiting ..
  39. ncbi Caveolin-1 up-regulates IGF-I receptor gene transcription in breast cancer cells via Sp1- and p53-dependent pathways
    Chen Glait
    Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Exp Cell Res 312:3899-908. 2006
    ..Finally, the effect of Cav-1 was associated with an elevation in the levels of Sp1, a zinc-finger protein with important roles in IGF-IR gene transactivation...
  40. pmc Progesterone receptor rapid signaling mediates serine 345 phosphorylation and tethering to specificity protein 1 transcription factors
    Emily J Faivre
    Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA
    Mol Endocrinol 22:823-37. 2008
    ..Ser345-phosphorylated PR-B receptors strongly associated with specificity protein 1 (Sp1) transcription factors to regulate PR cell cycle (p21) and growth-promoting (EGFR) target genes whose ..
  41. doi Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1
    Shayne A Bellingham
    Department of Biochemistry and Molecular Biology, The University of Melbourne, Victoria, Australia
    J Biol Chem 284:1291-301. 2009
    ..We hypothesized that transcription factors SP1 and metal transcription factor-1 (MTF-1) may be involved in copper-mediated regulation of human prion gene...
  42. ncbi Hypoxia-induced activation of the retinoic acid receptor-related orphan receptor alpha4 gene by an interaction between hypoxia-inducible factor-1 and Sp1
    Naoki Miki
    Laboratory of Genomics and Proteomics, Faculty of Pharmacy and Pharmaceutical Science, Fukuyama University, 1 Gakuen cho, Fukuyama 729 0292, Japan
    J Biol Chem 279:15025-31. 2004
    ..However, HIF-1 alone was not sufficient to activate transcription in hypoxic conditions but, rather, required Sp1/Sp3, which binds to a cluster of GC-rich sequences adjacent to the HRE...
  43. ncbi Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1.Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter
    Benoît Porée
    Laboratoire de Biochimie du Tissu Conjonctif, Universite de Caen Basse Normandie, IFR ICORE 146, Faculte de Medecine, CHU niveau 3, Avenue de la Cote de Nacre, 14032 Caen Cedex, France
    J Biol Chem 283:4850-65. 2008
    ..The cytokine and/or sIL-6R repress COL2A1 transcription by a -63/-35 sequence that binds Sp1.Sp3. Indeed, IL-6 and/or sIL-6R inhibit Sp1 and Sp3 expression and their binding activity to the 63-bp promoter...
  44. ncbi p53 regulates insulin-like growth factor-I (IGF-I) receptor expression and IGF-I-induced tyrosine phosphorylation in an osteosarcoma cell line: interaction between p53 and Sp1
    C Ohlsson
    Diabetes Branch, National Institute of Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1770, USA
    Endocrinology 139:1101-7. 1998
    ..A functional and physical interaction between p53 and Sp1, in the regulation of the IGF-R, was studied in osteosarcoma cells...
  45. ncbi Sp1 increases expression of cyclooxygenase-2 in hypoxic vascular endothelium. Implications for the mechanisms of aortic aneurysm and heart failure
    Q Xu
    Section on Cardiology, Department of Medicine, and Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1045, USA
    J Biol Chem 275:24583-9. 2000
    ..b>Sp1 is a transactivation factor known to be important in the regulation of cytokine expression in association with NF-..
  46. ncbi Sp1 and TAFII130 transcriptional activity disrupted in early Huntington's disease
    Anthone W Dunah
    Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Center for Aging, Genetics and Neurodegeneration, Charlestown, MA 02129, USA
    Science 296:2238-43. 2002
    ..Here, we report that huntingtin interacts with the transcriptional activator Sp1 and coactivator TAFII130...
  47. ncbi Crucial roles of Sp1 and epigenetic modifications in the regulation of the CLDN4 promoter in ovarian cancer cells
    Hiroshi Honda
    Laboratory of Cellular and Molecular Biology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 281:21433-44. 2006
    ..This region contains two Sp1 sites, both of which are required for promoter activity...
  48. doi Sp1 is upregulated in human glioma, promotes MMP-2-mediated cell invasion and predicts poor clinical outcome
    Hongyu Guan
    Department of Endocrinology and Diabetes Center, the First Affiliated Hospital of Sun Yat Sen University, Guangzhou, Guangdong, China
    Int J Cancer 130:593-601. 2012
    b>Sp1, the first identified transcription factor, has been reported to be associated with the development and progression of various human cancer types...
  49. doi Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression
    Yi Ting Wang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
    J Mol Biol 414:1-14. 2011
    Although Sp1 is known to undergo posttranslational modifications such as phosphorylation, glycosylation, acetylation, sumoylation, and ubiquitination, little is known about the possible interplay between the different forms of Sp1 that ..
  50. ncbi COUP-TF and Sp1 interact and cooperate in the transcriptional activation of the human immunodeficiency virus type 1 long terminal repeat in human microglial cells
    O Rohr
    Unité 338 INSERM, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France
    J Biol Chem 272:31149-55. 1997
    ..This region is a direct target of transcription factors Sp1 and Sp3. We report the discovery and features of a physical and functional interplay between COUP-TF and Sp1...
  51. ncbi Interaction of p38 and Sp1 in a mechanical force-induced, beta 1 integrin-mediated transcriptional circuit that regulates the actin-binding protein filamin-A
    Mario D'Addario
    Canadian Institutes of Health Research Group in Matrix Dynamics, University of Toronto, Ontario, Canada
    J Biol Chem 277:47541-50. 2002
    ..the redistribution of p38 to the integrin/bead locus and the nucleus as well as enhanced binding of the transcription factor Sp1 to proximal, regulatory domains of the filamin-A promoter...
  52. ncbi Regulation of KiSS-1 metastasis suppressor gene expression in breast cancer cells by direct interaction of transcription factors activator protein-2alpha and specificity protein-1
    Dianne C Mitchell
    Institute of Biosciences and Technology, Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, Houston 77030, USA
    J Biol Chem 281:51-8. 2006
    ..directly with the expression levels of two transcription factors, activator protein-2alpha (AP-2alpha) and specificity protein 1 (Sp1), which synergistically activate the transcriptional regulation of KiSS-1 in breast cancer cells...
  53. ncbi Sp1 family of transcription factors regulates the human alpha2 (XI) collagen gene (COL11A2) in Saos-2 osteoblastic cells
    Tomohiro Goto
    Department of Orthopaedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
    J Bone Miner Res 21:661-73. 2006
    ..By studying Saos-2 cells, we showed that the transcription factors, Sp1, Sp3, and Sp7 (Osterix), regulate COL11A2 expression through its proximal promoter...
  54. ncbi A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening
    M Gunther
    Laboratoire de génétique oncologique, UMR 1599 CNRS, Institut Gustave Roussy, France
    Mol Cell Biochem 210:131-42. 2000
    ..cDNA clones encoding polypeptides that interact with the N-terminal region (activation domains A, B and C) of the Sp1 transcription factor. Among the 65 collected clones, 43 contained cDNA fragments with open reading frames...
  55. ncbi Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1
    Adriana De Siervi
    Molecular Therapeutics Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 64:743-50. 2004
    ..The minimal p21 promoter region required for perifosine-induced p21 promoter activation contains four consensus Sp1-binding sites. Mutations in each particular Sp1 site block perifosine-induced p21(waf1/cip1) expression...
  56. pmc Sp1 is involved in Akt-mediated induction of VEGF expression through an HIF-1-independent mechanism
    Nabendu Pore
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19004, USA
    Mol Biol Cell 15:4841-53. 2004
    ..using reporter constructs containing the VEGF promoter showed that up-regulation of VEGF by Akt is mediated through Sp1 binding sites located in the proximal promoter...
  57. ncbi Molecular mechanism of inhibitory aryl hydrocarbon receptor-estrogen receptor/Sp1 cross talk in breast cancer cells
    Shaheen Khan
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, 4466 TAMU, College Station, Texas 77843 4466, USA
    Mol Endocrinol 20:2199-214. 2006
    ..pCAD) containing hormone-responsive GC-rich CAD promoter inserts involves estrogen receptor alpha (ERalpha)/Sp1 interactions with these proximal GC-rich motifs...
  58. ncbi The human immunodeficiency virus-1 Tat protein increases cell proliferation, alters sensitivity to zinc chelator-induced apoptosis, and changes Sp1 DNA binding in HeLa cells
    M Seve
    Laboratoire de Biologie du Stress Oxydant LBSO, University of Grenoble, La Tronche, 38700, France
    Arch Biochem Biophys 361:165-72. 1999
    ..changes enhance proliferation and apoptosis and alter the activity of zinc thiolate-containing proteins such as Sp1. Cells stably producing the Tat protein have an increased proliferation rate, which can be inhibited by ..
  59. ncbi Transcriptional repression of protein kinase Calpha via Sp1 by wild type p53 is involved in inhibition of multidrug resistance 1 P-glycoprotein phosphorylation
    Maocheng Zhan
    Department of Surgical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 280:4825-33. 2005
    ..Deletion and mutation analysis of the PKCalpha promoter fused to the luciferase reporter gene identified a Sp1 binding site (-244/-234) in the PKCalpha promoter that is required for p53-mediated inhibition of PKCalpha promoter ..
  60. ncbi E2F suppression and Sp1 overexpression are sufficient to induce the differentiation-specific marker, transglutaminase type 1, in a squamous cell carcinoma cell line
    Chung Fai Wong
    Epithelial Pathobiology Group, Cancer Biology Programme, Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia
    Oncogene 24:3525-34. 2005
    ..We extend these findings to report that in HEKs, Sp1 is a differentiation-specific activator and a downstream target of E2F-mediated suppression of the differentiation-..
  61. ncbi Investigation on the role of cell transcriptional factor Sp1 and HIV-1 TAT protein in PML onset or development
    M Mischitelli
    Department of Public Health Sciences, University of Rome La Sapienza, Italy
    J Cell Physiol 204:913-8. 2005
    ..positive and negative subjects both with PML and after sequencing, we analyzed the viral variants found focusing on Sp1 binding sites (box B and D) and up-TAR sequence (box C)...
  62. pmc TGFβ1 enhances MAD1 expression and stimulates promoter-bound Pol II phosphorylation: basic functions of C/EBP, SP and SMAD3 transcription factors
    Nadine Hein
    Institute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, 52057 Aachen, Germany
    BMC Mol Biol 12:9. 2011
    ..Moreover we have recently demonstrated that the MAD1 promoter is controlled by the cytokine granulocyte colony-stimulating factor (G-CSF) through the activation of STAT3, MAP kinases and C/EBP transcription factors...
  63. doi MicroRNA-21 represses human cystathionine gamma-lyase expression by targeting at specificity protein-1 in smooth muscle cells
    Guangdong Yang
    School of Kinesiology, Lakehead University, Thunder Bay, Canada
    J Cell Physiol 227:3192-200. 2012
    ..CSE/H(2)S system contributes to the maintenance of SMC phenotype, and transcript factor specificity protein-1 (SP1) is a critical regulator of CSE expression during SMC differentiation...
  64. doi Elevated transcription factor specificity protein 1 in autistic brains alters the expression of autism candidate genes
    Ismail Thanseem
    Department of Psychiatry and Neurology, Hamamatsu University School of Medicine, Japan
    Biol Psychiatry 71:410-8. 2012
    ..can result from abnormalities in the concentrations of sequence-specific transcription factors like specificity protein 1 (Sp1)...
  65. doi Transcription of the LAT gene is regulated by multiple binding sites for Sp1 and Sp3
    Caitlyn Whitten
    Agnes Scott College, Department of Biology, 141 E College Ave, Decatur, GA 30030, USA
    Gene 413:58-66. 2008
    ..5 GC-rich elements that contribute to promoter activity and that are capable of binding the transcription factors Sp1 and Sp3...
  66. doi Role of FOXA and Sp1 in mitochondrial acylcarnitine carrier gene expression in different cell lines
    Paolo Convertini
    Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Bari, Italy
    Biochem Biophys Res Commun 404:376-81. 2011
    ..Through deletion analysis, an activation domain (-334/-80 bp) was identified which contains FOXA and Sp1 active sites...
  67. doi Transcription factor Sp1 regulates basal transcription of the human DRG2 gene
    Hyo Jeong Kim
    Department of Biological Sciences, University of Ulsan, Ulsan 680 749, Korea
    Biochim Biophys Acta 1809:184-90. 2011
    ..Mutation of a putative stimulating protein 1 (Sp1) regulatory site located at position -108 resulted in a significant decline in DRG2 promoter activity...
  68. pmc Epigenetic regulation of phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger 1 gene expression in prostate cancer cells
    Chuu Yun A Wong
    Department of Pharmacology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
    J Biol Chem 286:25813-22. 2011
    ..One Sp1 site, essential for basal promoter activity, was identified as critical for the TSA effect...
  69. doi The proinflammatory cytokine, interleukin-6, up-regulates calcium-sensing receptor gene transcription via Stat1/3 and Sp1/3
    Lucie Canaff
    Department of Medicine, Physiology, and Human Genetics, McGill University, Quebec, Canada
    J Biol Chem 283:13586-600. 2008
    ..There are no Stat elements in the P2 promoter, but Sp1/3 elements are clustered at the transcription start site...
  70. doi Hepatitis B virus-X protein recruits histone deacetylase 1 to repress insulin-like growth factor binding protein 3 transcription
    Jin Kyung Shon
    Liver Cell Signal Transduction Laboratory, Metabolic Syndrome Research Center, Korea Research Institute of Bioscience and Biotechnology, Liver Research Division, Lee s Biotechnology Institute of Research and Development, Taejeon 305 606, Republic of Korea
    Virus Res 139:14-21. 2009
    ..HBx recruited HDAC1 forms complex with Sp1 in a p53-independent manner) and deacetylates Sp1 which resulted in the diminished binding of Sp1 on targeted DNA ..
  71. ncbi Butyrate, a histone deacetylase inhibitor, activates the human IGF binding protein-3 promoter in breast cancer cells: molecular mechanism involves an Sp1/Sp3 multiprotein complex
    G E Walker
    Department of Pediatrics, Oregon Health Sciences University, Portland, Oregon 97201, USA
    Endocrinology 142:3817-27. 2001
    ..identified key sodium butyrate-responsive element(s) to a 45-bp region containing consensus binding sites for Sp1 and activating protein-2...
  72. pmc Myc represses the p21(WAF1/CIP1) promoter and interacts with Sp1/Sp3
    A L Gartel
    Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 98:4510-5. 2001
    ..This region contains multiple Sp1-binding sites and a potential initiator element, but no canonical Myc DNA-binding sites...
  73. ncbi Association between expression of transcription factor Sp1 and increased vascular endothelial growth factor expression, advanced stage, and poor survival in patients with resected gastric cancer
    James C Yao
    Department of Gastrointestinal Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas, USA
    Clin Cancer Res 10:4109-17. 2004
    ..We evaluated the relationship between Sp1 and vascular endothelial growth factor (VEGF) expression, as well as their effect on survival in 86 cases of ..
  74. pmc Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus up-regulates transcription of human telomerase reverse transcriptase promoter through interaction with transcription factor Sp1
    Subhash C Verma
    Department of Microbiology and Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 78:10348-59. 2004
    ..The hTERT promoter sequence located at positions -130 to +5 contains several Sp1 binding motifs and was shown be important for up-regulation by LANA...
  75. pmc Control of stochastic gene expression by host factors at the HIV promoter
    John C Burnett
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California, United States of America
    PLoS Pathog 5:e1000260. 2009
    ..The HIV-1 promoter contains cis-acting Sp1 and NF-kappaB elements that regulate gene expression via the recruitment of both activating and repressing ..
  76. ncbi O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation
    S P Jackson
    Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720
    Cell 55:125-33. 1988
    ..Here we show that the human RNA polymerase II transcription factor Sp1 bears multiple O-linked N-acetylglucosamine (GlcNAc) monosaccharide residues...
  77. ncbi Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat
    L Wang
    Marion Merrell Dow Foundation, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160 7424, USA
    J Biol Chem 270:25564-9. 1995
    ..a minimal promoter from HIV-1 LTR containing the TATA box and binding motifs for the ubiquitous cellular transcription factor Sp1. Evidence is presented that Vpr interacts with Sp1 when Sp1 is bound to the Sp1 motifs within the HIV-1 ..
  78. ncbi The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2
    Jian Min Sun
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 277:35783-6. 2002
    b>Sp1 and Sp3 are ubiquitously expressed mammalian transcription factors that function as activators or repressors...
  79. ncbi TGF-beta-induced expression of tissue inhibitor of metalloproteinases-3 gene in chondrocytes is mediated by extracellular signal-regulated kinase pathway and Sp1 transcription factor
    Hamid Yaqoob Qureshi
    Department of Medicine and Centre de Recherche du Centre Hospitalier de l Université de Montréal CRCHUM, Montreal, Quebec, Canada
    J Cell Physiol 203:345-52. 2005
    ..investigated the role of extracellular-signal-regulated kinase (ERK)-mitogen-activated protein kinases (MAPK) and Sp1 transcription factor in TGF-beta-induced TIMP-3 gene in chondrocytes and chondrosarcoma cells...
  80. pmc Sp1 deacetylation induced by phorbol ester recruits p300 to activate 12(S)-lipoxygenase gene transcription
    Jan Jong Hung
    Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
    Mol Cell Biol 26:1770-85. 2006
    We previous reported that Sp1 recruits c-Jun to the promoter of the 12(S)-lipoxygenase gene in 12-myristate 13-acetate-treated cells...
  81. ncbi Hypoxic condition- and high cell density-induced expression of Redd1 is regulated by activation of hypoxia-inducible factor-1alpha and Sp1 through the phosphatidylinositol 3-kinase/Akt signaling pathway
    Hyeon Ok Jin
    Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, 215 4 Gongneung dong, Nowon Gu, Seoul 139 706, Republic of Korea
    Cell Signal 19:1393-403. 2007
    ..agent, cobalt chloride (CoCl(2)) and high cell density (HCD) requires coactivation of HIF-1alpha and Sp1. CoCl(2) and HCD induced the activation of HIF-1alpha and Sp1 in HeLa cells, and siRNAs targeting HIF-1alpha and ..
  82. ncbi Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation
    N Billon
    Differentiation and Cell Cycle Group, Laboratoire de Biologie Moleculaire et Cellulaire, UMR 49 CNRS Ecole Normale Supérieure de Lyon, France
    Oncogene 18:2872-82. 1999
    ..This GC-rich region was shown to interact specifically with the transcription factor Sp1 and the related protein Sp3, in either exponentially-growing or NGF-treated PC12 cells...
  83. doi Sp1 coordinately regulates de novo lipogenesis and proliferation in cancer cells
    Suying Lu
    Department of Nutritional Sciences, University of Toronto, ON, Canada
    Int J Cancer 126:416-25. 2010
    ..How cancer cells coordinate de novo lipogenesis and proliferation has not been investigated. Transcription factors Sp1, Sp3 and Sp4 are overexpressed in a variety of cancers and regulate gene expression by interacting with GC-rich Sp1 ..
  84. pmc Specificity protein 1 is pivotal in the skin's antiviral response
    Lianghua Bin
    Department of Pediatrics, National Jewish Health, Denver, Colo, USA
    J Allergy Clin Immunol 127:430-438.e1-2. 2011
    Previous studies have found specificity protein (Sp) 1 transcription factor in the viral replication machinery and postulated that Sp1 was required for viral replication in host cells.
  85. ncbi Functional interactions between Sp1 or Sp3 and the helicase-like transcription factor mediate basal expression from the human plasminogen activator inhibitor-1 gene
    H Ding
    Center for Molecular and Vascular Biology, University of Leuven, 3000 Leuven, Belgium
    J Biol Chem 274:19573-80. 1999
    ..a set of B box point mutants demonstrated two HLTF sites flanking and partially overlapping with a GT box binding Sp1 and Sp3. Mutations affecting either the Sp1/Sp3 or the two HLTF sites inhibited by 6- and 2...
  86. ncbi Role of Smad proteins and transcription factor Sp1 in p21(Waf1/Cip1) regulation by transforming growth factor-beta
    K Pardali
    Ludwig Institute for Cancer Research, Box 595, SE 751 24 Uppsala, Sweden
    J Biol Chem 275:29244-56. 2000
    ..Activation requires the binding sites for the ubiquitous transcription factor Sp1 on the proximal promoter...
  87. ncbi Sp1 and Smad proteins cooperate to mediate transforming growth factor-beta 1-induced alpha 2(I) collagen expression in human glomerular mesangial cells
    A C Poncelet
    Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60611 3008, USA
    J Biol Chem 276:6983-92. 2001
    ..Here, we report that Sp1 binding is necessary for TGF-beta1-induced type I collagen mRNA expression...
  88. ncbi Expression of the hypoxia marker carbonic anhydrase IX is critically dependent on SP1 activity. Identification of a novel type of hypoxia-responsive enhancer
    Stefan Kaluz
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California at Irvine, California 92717, USA
    Cancer Res 63:917-22. 2003
    ..We identified PR5 in the CA9 promoter as another SP1/SP3-binding site...
  89. ncbi Estrogen regulates KiSS1 gene expression through estrogen receptor alpha and SP protein complexes
    Dali Li
    Institute of Biosciences and Technology, Texas A and M Health Science Center, 2121 West Holcombe Boulevard, Houston, TX 77030, USA
    Endocrinology 148:4821-8. 2007
    ..Deletion analysis of KiSS1 promoter indicates that the E(2)-regulated increase in promoter activity depends on the Sp1 sites of the proximal promoter region...
  90. pmc Sequential activation of hypoxia-inducible factor 1 and specificity protein 1 is required for hypoxia-induced transcriptional stimulation of Abcc8
    Seung Kyoon Woo
    Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, Maryland 21201 1595, USA
    J Cereb Blood Flow Metab 32:525-36. 2012
    ..a series of luciferase reporter assays studying the Abcc8 promoter revealed that binding sites for specificity protein 1 (Sp1), but not for HIF, were required for stimulation of Abcc8 transcription by HIF1α...
  91. pmc O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions
    M D Roos
    Department of Cell Biology, University of Alabama at Birmingham, 35294, USA
    Mol Cell Biol 17:6472-80. 1997
    ..Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not ..
  92. ncbi Composite co-activator ARC mediates chromatin-directed transcriptional activation
    A M Näär
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Nature 398:828-32. 1999
    ..chromatin-selective manner to potentiate synergistic gene activation by the transcriptional activators SREBP-1a and Sp1. Here we show that this activator-recruited cofactor (ARC) interacts directly with several different activators, ..
  93. pmc Histone deacetylase 1 can repress transcription by binding to Sp1
    A Doetzlhofer
    Institute of Molecular Biology, Vienna Biocenter, University of Vienna, Vienna, Austria
    Mol Cell Biol 19:5504-11. 1999
    The members of the Sp1 transcription factor family can act as both negative and positive regulators of gene expression. Here we show that Sp1 can be a target for histone deacetylase 1 (HDAC1)-mediated transcriptional repression...
  94. ncbi Regulation of interaction of the acetyltransferase region of p300 and the DNA-binding domain of Sp1 on and through DNA binding
    T Suzuki
    Laboratory of Developmental Biology, Department of Cellular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Genes Cells 5:29-41. 2000
    ..However, one central question which has remained unanswered is how the coactivator/acetyltransferase interacts with DNA-binding activators to modulate their actions...
  95. ncbi Trichostatin A, a histone deacetylase inhibitor, activates the IGFBP-3 promoter by upregulating Sp1 activity in hepatoma cells: alteration of the Sp1/Sp3/HDAC1 multiprotein complex
    Hong Seok Choi
    Liver Cell Signal Transduction Lab, Bioscience Research Division, Korea Research Institute of Bioscience and Biotechnology, Taejon 305 606, Republic of Korea
    Biochem Biophys Res Commun 296:1005-12. 2002
    ..Promoter mutation analysis confirmed that the TSA-responsive element coincides with the Sp1/GC-rich region on the IGFBP-3 promoter...
  96. ncbi Tat and trans-activation-responsive (TAR) RNA-independent induction of HIV-1 long terminal repeat by human and murine cyclin T1 requires Sp1
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 278:6404-10. 2003
    ..We further showed that Sp1 is necessary and sufficient for this DNA-targeted activity...
  97. pmc Human immunodeficiency virus type 1 (HIV-1) Vpr enhances expression from unintegrated HIV-1 DNA
    Betty Poon
    Department of Microbiology, David Geffen School of Medicine at UCLA, UCLA AIDS Institute and Jonsson Comprehensive CAncer Center, Los Angeles, California 90095, USA
    J Virol 77:3962-72. 2003
    ..These results attribute a new function to HIV-1 Vpr and implicate Vpr as a critical component in expression from unintegrated HIV-1 DNA...
  98. pmc MCAF mediates MBD1-dependent transcriptional repression
    Naoyuki Fujita
    Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto University, Kumamoto 860 0811, Japan
    Mol Cell Biol 23:2834-43. 2003
    ..MCAF harbors two conserved domains that allow it to interact with MBD1 and enhancer-like transactivator Sp1. MCAF possesses a coactivator-like activity, and it seems to facilitate Sp1-mediated transcription...
  99. ncbi Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of IL-10 in mouse macrophages
    Yi Wen Liu
    Graduate Institute of Biopharmaceutics, College of Life Science, National Chiayi University, Chiayi, Taiwan
    J Immunol 171:821-8. 2003
    ..have revealed that LPS can activate transcription of the IL-10 gene promoter through an SV40 promoter factor 1 (Sp1) binding site in mouse macrophage RAW264.7...
  100. ncbi bcl-2 induction of urokinase plasminogen activator receptor expression in human cancer cells through Sp1 activation: involvement of ERK1/ERK2 activity
    Daniela Trisciuoglio
    Experimental Chemotherapy Laboratory, Regina Elena Cancer Institute, Via delle Messi d Oro 156, 00158 Rome, Italy
    J Biol Chem 279:6737-45. 2004
    ..in cancer cells exposed to hypoxia modulates urokinase plasminogen activator receptor (uPAR) expression through Sp1 transcription factor and that the extracellular signal-regulated kinase (ERK) pathway plays a role in Sp1 ..
  101. ncbi NF-kappaB site interacts with Sp factors and up-regulates the NR1 promoter during neuronal differentiation
    Anguo Liu
    Department of Biomedical Sciences, Dental School, Program in Neuroscience, and Program in Cellular and Molecular Biology, University of Maryland, Baltimore, Maryland 21201, USA
    J Biol Chem 279:17449-58. 2004
    ..Interestingly, the NR1 NF-kappaB site strongly interacted with Sp3/Sp1, instead of NF-kappaB factors, in P19 nuclear extracts...

Research Grants63

  1. Melissa Churchill; Fiscal Year: 2014
    ..peripheral blood mononuclear cells (PBMC) of the same subjects, and are defined by alterations associated with the Sp1 transcription factor binding motif...
  2. Enhance Production of Functional Recombinant Human Protein C Variant In Mammalian
    Nienwen Chow; Fiscal Year: 2010
    ..SOD1G85R or SOD1G37R mutants via protease activated receptors 1 and 3, which inhibits nuclear transport of the SP1 transcription factor...
  3. Ashok Kumar; Fiscal Year: 2016
    ..Hypothesis 3: Demethylation of CpG islands in Fn14 promoter followed by binding of SP1 transcription factor triggers the expression of Fn14 in skeletal muscle in catabolic states...
  4. Jean Pierre J Issa; Fiscal Year: 2016
    ..features and known factors associated with differential predisposition to DNA methylation (the transcription factor Sp1, LINE/SINE retrotransposons and the insulator proteins CTCF, USF1/2 and VEZF1) will be tested in specific ..
  5. Dietary HDAC Inhibitors in Colon Cancer Prevention
    Roderick H Dashwood; Fiscal Year: 2012
    ..Potent HDAC inhibitors `de- repress'epigenetically silenced genes, such as P21/WAF1, through Sp1/Sp3 transcription factor binding sites in the corresponding gene promoters, causing growth arrest/apoptosis in ..
  6. Transcriptional regulation of enteroendocrine cell differentiation by NeuroD
    Andrew B Leiter; Fiscal Year: 2013
    ..Aim 1 will examine how NeuroD associates with Sp1, RREB1, and LSD1 at one its known targets, the secretin gene to form a multiprotein coactivator complex...
  7. Susana T Valente; Fiscal Year: 2016
    ..Upon HIV infection, DNA-PK tightly associates with Tat to promote phosphorylation of the transcription factor Sp1, which results in increased HIV-1 transcription initiation, via three Sp1 binding sites in the core of ..
  8. Carole R Mendelson; Fiscal Year: 2016
    ..1) and nuclear factor (B (NF-(B), upstream stimulatory factors (USFs) 1 and 2, and Sp1 to critical response elements upstream of the SP-A gene...
  9. SPORE in Pancreatic Cancer
    James Abbruzzese; Fiscal Year: 2009
    ..Project 4 - Regulation of Pancreatic Cancer Angiogenesis and Metastasis by Transcription Factor Sp1 will examine the hypothesis that disregulated expression and activation of Sp1 is responsible for ..
  10. Sp1, kappa-B enhancers and transcriptions in neurons
    STEVEN BARGER; Fiscal Year: 2009
    ..Furthermore, the only factors constitutively binding NF-kappa-B target sites are Sp1 and related proteins, and glutamate decreases this activity...
  11. Nitric oxide regulation of CD55 and infection
    Chandrasekhar Yallampalli; Fiscal Year: 2013
    ..1.1. We will determine, by site directed mutations, the role of CREB, AP1, AP2, and SP1 binding sites at -74 to -43 region of CD55 promoter in the NO modulated transcription of CD55. 1.2...
  12. CTCF peptides - a new therapeutic alternative to target HIV in the brain.
    Malgorzata Simm; Fiscal Year: 2012
    ..CTCF from nuclear extracts of HRF(+) but not HRF(-) control cells bound to DNA probe comprising NF-[unreadable]B, Sp1 and YB-1 binding sequences, possibly through its interaction with YB-1 protein...
  13. Regulation of the Expression of G[alpha]i2 by Reactive Oxygen Species
    Ifeanyi J Arinze; Fiscal Year: 2012
    ..the hypothesis that protein-protein interaction between C/EBP1 and one or more of the other transcription factors (Sp1, Nrf2, and NF-kB) that bind at the ROS-responsive region of the G1i2 gene promoter constitute the mechanistic basis ..
  14. Marshall B Elam; Fiscal Year: 2014
    ..and have found that its full response requires participation of multiple cis-acting sites that bind to LXR1, NF-Y, Sp1 and SREBP-1c itself...
  15. Role of aberrant organelle stress responses in alcohol-induced liver injury
    Cheng Ji; Fiscal Year: 2012
    ..factors (XBP-1, CHOP, ATF6, ATF4, CREB, TORC3, PGC-1a), general transcription factors (Pol II, NF-Y, NFR, Sp1, TBP and p300) and epigenetic marks (methylation of H3-K4 and H3-K79 and acetylation of H3 and H4) in promoters of ..
  16. Gerald Warren Hart; Fiscal Year: 2014
    ..Specific Aim 3. Elucidate the Roles of GlcNAcylation of the General Transcription Factor Sp1 in its Activity, Promoter Specificity, Localization, Molecular Associations and Turnover. a...
  17. Mechanism of Heat Shock Protein Induction by Glutamine
    Paul E Wischmeyer; Fiscal Year: 2012
    ..of the O-GlcNAc pathway and nuclear translocation and activation of key inducers of the HSP pathway, such as Sp1 and heat shock factor-1 (HSF-1)...
  18. J Marc Simard; Fiscal Year: 2016
    ..We will determine the early activation of the mechano-sensitive transcription factors, specificity protein 1 (Sp1) and nuclear factor kappaB (p65), and the time-course and cellular localization for the cell death ..
  19. Colon Cell Differentiation: NAB and MUC2 Gene Expression
    Anna Velcich; Fiscal Year: 2009
    ..2. Whether HDAC activity can counteract NaB mediated repression of MUC2 3. Whether there is modulation of SP1/SP3 activity, which we demonstrated is important for MUC2 regulation. 4...
  20. MECHANISMS OF NA+ TRANSPORT IN THE HUMAN INTESTINE
    Pradeep K Dudeja; Fiscal Year: 2012
    ..We have cloned the human NHE2 cDNA and its promoter region and established that transcription factors, Sp1 and Sp3, play a major role in the basal and stimulated NHE2 promoter activity...
  21. ANUMANTHA GOUNDER KANTHASAMY; Fiscal Year: 2015
    ..Further analysis of the PKCd promoter revealed that two key transcription factors, NFkB and SP1, regulate PKCd gene expression...
  22. Jane R Taylor; Fiscal Year: 2014
    ..selected drug-induced neurobiological alterations in the OFC focusing on cAMP-regulated signaling and the transcription factor Sp1 that was identified in our proteomic analyses...
  23. NASSER HUSSEIN ZAWIA; Fiscal Year: 2014
    ..The transcription factor specificity protein 1 (Sp1) is essential for the regulationof the tau gene and its cyclin dependent kinase 5 (CDK5)...
  24. Targeting myofibroblast activation in chronic fibrotic disease
    ARTHUR ROGER STRAUCH; Fiscal Year: 2011
    ..of YB-1 and Pur protein repressors with SMalphaA and collagen promoter DNA and the transcriptional activators Sp1, SRF, and Smads 2,3, delineate regions of repressor polypeptide chains required for this functional interplay, and ..
  25. Mechanisms of Neuronal Specific Transcription
    Grace Gill; Fiscal Year: 2009
    ..hypothesis that the mechanisms that regulate the levels and activity of the GC box-binding transcription factors Sp1, Sp3 and Sp4 during neurogenesis contribute to neuronal-specific expression of p35...
  26. Charles E Samuel; Fiscal Year: 2014
    ..To delineate the importance of Sp1 and Sp3 protein binding to the novel 15-bp DNA element and upstream sites in IFN inducible as well as basal ..
  27. MECHANISTIC AND TRANSLATIONAL STUDIES OF THE HYPOXIA RESPONSE IN CANCER
    Garth Powis; Fiscal Year: 2011
    ..We have used transcription factor profiling to identify two other transcription factors, Sp1 and Nrf2, that play an independent role in the hypoxia response and will study their mechanisms of action...
  28. Regulation of VEGF/HIF-1 by AKT: Implications for Radiotherapy
    Amit Maity; Fiscal Year: 2010
    ..First, the PI3K/AKT pathway increases transcription by increasing binding of the transcription factor Sp1 to the VEGF proximal core promoter...
  29. CAPSAICIN RECEPTOR SUBTYPES IN PAIN TRANSDUCTION
    Mark A Schumacher; Fiscal Year: 2012
    ..Based on additional data, we now propose to study a link between the Sp1- like family of transcription factors and NGF / inflammation mediated increases in TRPV1 dependent pain...
  30. Role of Daf in Systemic Autoimmunity
    KENNETH MICHAEL POLLARD; Fiscal Year: 2012
    ..and negative regulatory regions, and basal transcriptional activity that requires functional cooperation of Sp1-binding sites...
  31. Shujun Liu; Fiscal Year: 2014
    ..hypothesis is that there exists a synergistic effect among DNA hypomethylating agents (bortezomib, miR29b and Sp1 siRNA) when used as combination therapy and this synergism may be enhanced when delivered by liposome- or ..
  32. ABNORMAL BIGH3 AGGREGATIONS IN CORNEAL DYSTROPHIES
    Andrew J W Huang; Fiscal Year: 2011
    ..To further determine the TGFb-mediated gene activation, transcriptional factors such as Sp1 and Smads responsible for bIGH3 gene activation in corneas will be characterized using in vivo UV photofootprinting ..
  33. MOLECULAR REGULATION OF SMOOTH MUSCLE DIFFERENTIATION
    Gary K Owens; Fiscal Year: 2010
    ..suppresses expression of SMC differentiation marker genes and will include investigation of the role of KLF4, NFkB, Sp1, and inhibition of CArG-SRF-myocardin dependent transcription...
  34. Zijie Sun; Fiscal Year: 2016
    ..Furthermore, our recent data showing that the AR represses Sp1-induced c-Met transcription suggest a dual regulatory role for AR in transcription...
  35. Transcriptional regulation of apoptosis
    James J Manfredi; Fiscal Year: 2013
    ..basis for the differences between the two types of p53 sites will be elucidated and the interplay between p53, Sp1, and other Sp/KLF family members will be examined...
  36. Mass spectrometry of proteome, phosphoproteome and phosphatase-targeted drug desi
    Yuri Obukhov; Fiscal Year: 2012
    ..Specifically, we will analyze PP1-associated protein complexes that target PP1 to CDK9, Sp1 or RNAPII and other yet unknown proteins...
  37. BIOCHEMICAL ANALYSIS OF FACTORS ASSOCIATED WITH TFIID
    Naoko Tanese; Fiscal Year: 1999
    ..Preliminary studies suggest that hTAF130 binds to the human transcription factor Sp1 in vitro...
  38. Sp1 As a Novel Protective Mechanism Against Septic inflammation And Organ Injury.
    Shu Fang Liu; Fiscal Year: 2010
    ..We recently discovered that endotoxemia or sepsis down-regulates Sp1 (specificity protein 1) DNA binding activity and depletes tissue Sp1 protein in rat and mouse lungs, which are accompanied by a ..
  39. Bioactive compound modulation of epigenetic regulator Sp1/NFkB/miR network in AML
    Shujun Liu; Fiscal Year: 2012
    ..Thymoquinone (TQ) may achieve higher efficacy of DNA hypomethylation through modulating methylation regulator Sp1/NFkB/miR network...
  40. Guido Marcucci; Fiscal Year: 2016
    ..In preclinical studies, we showed that endogenous miR-29b can be pharmacologically increased with TKI (sorafenib), Sp1/NFkB interfering compounds (bortezomib) and histone deacetylase inhibitors...
  41. REGULATION OF ESTROGEN-RESPONSIVE GENES
    ANN NARDULLI; Fiscal Year: 2007
    ..To understand how estrogen-responsive genes containing AP-1 and Sp1 sites but lacking EREs respond to hormone treatment, we will study the regulation of the endogenous progesterone ..
  42. Prevention of acute GvHD by inhibition of cdk2
    Vassiliki A Boussiotis; Fiscal Year: 2012
    ..Cdk2 directly regulates expression of genes including NF?B, Sp1, p300/CBP, and subunits of the RNA polymerase...
  43. Pranoti Mandrekar; Fiscal Year: 2016
    ..alcohol induces hsp90 in macrophages by: A) Characterization of the binding of HSF-1, NFkB, stimulatory protein-1 (Sp1) and STAT1 by chromatin immunoprecipitation analysis...
  44. Wanli W Smith; Fiscal Year: 2014
    ..Through work examining the phenotype of a synphillin-1 transgenic mouse (SP1), we have evidence that synphilin-1 over- expression significantly increases body weight and does so through ..
  45. GIANFRANCO D ALPINI; Fiscal Year: 2016
    ..large cholangiocyte phenotypes such as the expression and synthesis of secretin (through activation of NeuroD1 and SP1), and secretin receptor (by activation of CaMK I and the adenylyl cyclase, AC8, and the subsequent activation of ..
  46. Serotonin-limbic system interactions that mediate anxiety
    Sheryl G Beck; Fiscal Year: 2013
    ..The following Specific Aims (SA) will test this hypothesis. SP1: To determine the developmental profile of layer 5 pyramidal neurons in the mPFC in concert with responses elicited ..
  47. KLF4, myeloid development, and transplantation arteriosclerosis
    A K M KHYRUL WARA; Fiscal Year: 2010
    ..Our preliminary studies indicate that KLF4 induces monocyte differentiation in part by synergizing with Sp1, whereas it inhibits granulocyte differentiation through an antagonistic interaction with C/EBP-a...
  48. Regulation of the Salmonella Pathogenicity Island 1 Type III Secretion System
    James M Slauch; Fiscal Year: 2013
    ..The regulation of the SP1 T3SS serves as a paradigm for the integration of host environmental signals to control virulence gene expression ..
  49. Geoffrey W Abbott; Fiscal Year: 2015
    ..AF and Kv channel remodeling, how these mechanisms mirror post-operative AF in larger animals, and the role of Sp1, miR-1 and miR-133 in this remodeling...
  50. Ashok K Saluja; Fiscal Year: 2016
    ..data suggest that triptolide induces ER stress and inhibits NFkB by downregulating Specificity protein1 (Sp1)...
  51. HYPERTENSION IN AFRICAN-AMERICAN WOMEN
    Ashok Kumar; Fiscal Year: 2004
    ..and we have shown that liver enriched transcription factors HNF4 and DBP, and ubiquitous transcription factors SP1 and CREB play important role in liver specific expression of this gene...
  52. Directed Evolution of Selective Protease Inhibitors with an Expanded Genetic Code
    Jennifer L Furman; Fiscal Year: 2013
    ..system for the incorporation of unnatural amino acids into cyclic peptide inhibitors of a cell surface protease, MT-SP1 (matriptase)...
  53. TGF-Beta signaling in mesangial cell collagen expression
    Anne Christine Poncelet; Fiscal Year: 2010
    ..human mesangial cells and plays a role in TGF-beta1-induced type I collagen expression;and that Smad proteins and Sp1 cooperate to mediate TGF-beta1-increased alpha2(I) collagen (COL1A2) promoter activity...
  54. Confocal Microscope at NEPRC
    Wei Dong Yao; Fiscal Year: 2010
    ..The existing multi-user confocal system, a Leica TCS SP1 purchased in 1998, has served as a workhorse for us over 10 years and provided outstanding service to the broad ..
  55. Preparation and Characterization of 2nd Generation HIV-1 Maturation Inhibitor Dru
    Carl Wild; Fiscal Year: 2013
    ..HIV maturation inhibitors block virus replication by disrupting the conversion of the capsid precursor protein, CA-SP1 (p25), to the mature form of capsid, CA (p24) resulting in the formation of non-infectious viral particles...
  56. Michael B Winter; Fiscal Year: 2014
    ..In the research plan, protease-specific probes for two members of the TTSP family, MT-SP1 and TMPRSS2, are designed and implemented in order to better assess the functional roles of the proteases in ..
  57. KLF4 Genetic and Epigenetic Changes in Human Pancreatic Cancer
    Keping Xie; Fiscal Year: 2012
    ..Specific Aim 3 will test the hypothesis that altered KLF4 expression and function results in dysregulated Sp1 expression and the imbalanced expression and function of Sp1 and KLF4 leads to an increased expression of pro-..
  58. Regulation of PAF Acetylhydrolase Expression by Oxidized Phospholipids
    SUZANNE BARBOUR; Fiscal Year: 2009
    ..In Aim 2, we will investigate role of a GC-box, Sp1, and Sp3 in induction of PAFAH by oxPL and determine whether PAFAH induction is dependent on phosphorylation of Sp1/..
  59. Mechanism of action of an HIV-1 maturation inhibitor
    Feng Li; Fiscal Year: 2012
    ..a single step in the processing of the viral Gag protein: protease cleavage of the Gag capsid (CA) precursor (CA-SP1) to mature CA protein...
  60. CONTROL OF GENE TRANSCRIPTION BY ESSENTIAL FATTY ACIDS
    Thomas Gettys; Fiscal Year: 2005
    ..The -118/-43 PUFA-RRFAS involves SREBP-1, NF-Y and Sp1 binding and in this sense has similarities to the PUFA-RR of the S14 gene...
  61. Signaling Mechanisms Regulation Muscle Protein Turnover
    S Price; Fiscal Year: 2005
    ..2) Other Preliminary Results indicate the increase in ubiquitin transcription induced by glucocorticoids involves Sp1 and the MAP kinase pathway...
  62. DEVELOPMENTAL CONTROL OF IMMUNOGLOBULIN TRANSCRIPTION
    Michael Atchison; Fiscal Year: 2005
    ..1, Pip, E47, c-jun, c-fos, ATF1, CREM, Sp1, BSAP, and YY1)...
  63. CONTROL OF HIV-1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 2000
    ..The promoter activities of proviral HIV and HSV a4 (ICP4) gene are regulated by host protein Sp1 in addition to other cellular factors...