SRSF1

Summary

Gene Symbol: SRSF1
Description: serine and arginine rich splicing factor 1
Alias: ASF, SF2, SF2p33, SFRS1, SRp30a, serine/arginine-rich splicing factor 1, SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, splicing factor 2, splicing factor, arginine/serine-rich, 30-KD, A
Species: human

Top Publications

  1. pmc Functional domains of the human splicing factor ASF/SF2
    P Zuo
    Department of Biological Sciences, Columbia University, New York, NY 10027
    EMBO J 12:4727-37. 1993
  2. pmc Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative splicing factor
    Jeremy R Sanford
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 102:15042-7. 2005
  3. doi Subcutaneous fat shows higher thyroid hormone receptor-alpha1 gene expression than omental fat
    Francisco J Ortega
    Department of Diabetes, Endocrinology and Nutrition, Institut d Investigació Biomédica de Girona and CIBEROBN CB06 03 010, Instituto de Salud Carlos III, Girona, Spain
    Obesity (Silver Spring) 17:2134-41. 2009
  4. pmc Alternative splicing factor/splicing factor 2 regulates the expression of the zeta subunit of the human T cell receptor-associated CD3 complex
    Vaishali R Moulton
    Division of Rheumatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:12490-6. 2010
  5. pmc Role of cellular RNA processing factors in human immunodeficiency virus type 1 mRNA metabolism, replication, and infectivity
    Joseph A Jablonski
    Basic Science Department, College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
    J Virol 83:981-92. 2009
  6. pmc Chromatin dynamics associated with HIV-1 Tat-activated transcription
    Rebecca Easley
    The George Washington University Medical Center, Department of Microbiology, Immunology, and Tropical Medicine, Washington, DC 20037, USA
    Biochim Biophys Acta 1799:275-85. 2010
  7. pmc Interaction between the RNA binding domains of Ser-Arg splicing factor 1 and U1-70K snRNP protein determines early spliceosome assembly
    Suhyung Cho
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 108:8233-8. 2011
  8. pmc MicroRNA (miRNA)-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects mouse embryonic fibroblast senescence and apoptosis
    Lorena Verduci
    Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, 56124 Pisa, Italy
    J Biol Chem 285:39551-63. 2010
  9. doi The hepatitis delta virus RNA genome interacts with eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2
    Dorota Sikora
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Room 4111A, Ottawa, Ontario, Canada, K1H 8M5
    Virology 390:71-8. 2009
  10. doi Functional association of human Ki-1/57 with pre-mRNA splicing events
    Gustavo C Bressan
    Laboratorio Nacional de Luz Sincrotron, Campinas, SP, Brasil
    FEBS J 276:3770-83. 2009

Research Grants

  1. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
  2. Alternative Splicing of the Insulin Receptor Gene
    NICHOLAS J WEBSTER; Fiscal Year: 2013
  3. Ilker Kudret Sariyer; Fiscal Year: 2016
  4. VAISHALI MOULTON; Fiscal Year: 2016
  5. ASF/SF2: Phosphorylation and RNA Binding
    Gourisankar Ghosh; Fiscal Year: 2012
  6. Regulation of tumorigenic spliced isoforms in cancer
    Aixa Tapia Santos; Fiscal Year: 2010
  7. Gross Hall Animal Resource Center
    Susan V Bryant; Fiscal Year: 2010
  8. James L Manley; Fiscal Year: 2015
  9. Splicing factors as therapeutic targets for the treatment of ovarian cancer
    William T Beck; Fiscal Year: 2012
  10. BLANTON SMITH TOLBERT; Fiscal Year: 2016

Detail Information

Publications223 found, 100 shown here

  1. pmc Functional domains of the human splicing factor ASF/SF2
    P Zuo
    Department of Biological Sciences, Columbia University, New York, NY 10027
    EMBO J 12:4727-37. 1993
    The human splicing factor ASF/SF2 displays two predominant activities in in vitro splicing assays: (i) it is an essential factor apparently required for all splices and (ii) it is able to switch utilization of alternative 5' splice sites ..
  2. pmc Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative splicing factor
    Jeremy R Sanford
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 102:15042-7. 2005
    ..Here, we show that endogenous, cytoplasmic splicing factor 2/alternative splicing factor (SF2/ASF) associated with the translation machinery is hypophosphorylated, ..
  3. doi Subcutaneous fat shows higher thyroid hormone receptor-alpha1 gene expression than omental fat
    Francisco J Ortega
    Department of Diabetes, Endocrinology and Nutrition, Institut d Investigació Biomédica de Girona and CIBEROBN CB06 03 010, Instituto de Salud Carlos III, Girona, Spain
    Obesity (Silver Spring) 17:2134-41. 2009
    ..TR alpha, TR alpha 1, and TR alpha 2 mRNA levels, and the gene expressions of arginine-serine-rich, splicing factor 2 (SF2), heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1), heterogeneous nuclear ribonucleoprotein A1 (..
  4. pmc Alternative splicing factor/splicing factor 2 regulates the expression of the zeta subunit of the human T cell receptor-associated CD3 complex
    Vaishali R Moulton
    Division of Rheumatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:12490-6. 2010
    ..down" by a CD3zeta 3'-UTR oligonucleotide, which identified the splicing protein alternative splicing factor/splicing factor 2 (ASF/SF2)...
  5. pmc Role of cellular RNA processing factors in human immunodeficiency virus type 1 mRNA metabolism, replication, and infectivity
    Joseph A Jablonski
    Basic Science Department, College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
    J Virol 83:981-92. 2009
    ..virus, we altered the expression levels of hnRNPs H, F, 2H9, GRSF1, A1, A2, and A3 and SR proteins SC35, SF2, and SRp40 in HEK 293 cells transfected with the proviral clone pNL4-3...
  6. pmc Chromatin dynamics associated with HIV-1 Tat-activated transcription
    Rebecca Easley
    The George Washington University Medical Center, Department of Microbiology, Immunology, and Tropical Medicine, Washington, DC 20037, USA
    Biochim Biophys Acta 1799:275-85. 2010
    ..Finally, we cover recent literature on viral microRNAs which have been shown to alter chromatin structure by inducing methylation or even by remodeling nucleosomes...
  7. pmc Interaction between the RNA binding domains of Ser-Arg splicing factor 1 and U1-70K snRNP protein determines early spliceosome assembly
    Suhyung Cho
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 108:8233-8. 2011
    ..assembly event is mediated by the RNA recognition domains (RRM) of serine/arginine-rich splicing factor 1 (SRSF1), which bridges the RRM of U1-70K to pre-mRNA by using the surface opposite to the RNA binding site...
  8. pmc MicroRNA (miRNA)-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects mouse embryonic fibroblast senescence and apoptosis
    Lorena Verduci
    Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, 56124 Pisa, Italy
    J Biol Chem 285:39551-63. 2010
    ..Both miRNAs are predicted to target alternative splicing factor/splicing factor 2 (ASF/SF2), a serine/arginine protein essential for cell viability...
  9. doi The hepatitis delta virus RNA genome interacts with eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2
    Dorota Sikora
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Room 4111A, Ottawa, Ontario, Canada, K1H 8M5
    Virology 390:71-8. 2009
    ..Co-immunoprecipitation was used to confirm the interactions of eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2 with the right terminal stem-loop domain of HDV genomic RNA in vitro, and with both polarities of HDV RNA within ..
  10. doi Functional association of human Ki-1/57 with pre-mRNA splicing events
    Gustavo C Bressan
    Laboratorio Nacional de Luz Sincrotron, Campinas, SP, Brasil
    FEBS J 276:3770-83. 2009
    ..In summary, our findings suggest that Ki-1/57 is probably involved in cellular events related to RNA functions, such as pre-mRNA splicing...
  11. pmc Cellular protein modulates effects of human immunodeficiency virus type 1 Rev
    Y Luo
    Howard Hughes Medical Institute, San Francisco, California
    J Virol 68:3850-6. 1994
    ..YL2 has 92% identity to a human 32-kDa protein (p32), which copurifies with alternative splicing factor SF2/ASF. Furthermore, we found that whereas expression of YL2 greatly potentiated the activity of Rev, antisense YL2 ..
  12. ncbi In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32
    T O Tange
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 271:10066-72. 1996
    ..Here we show that the YL2 human homologue, the p32 protein, which co-purifies with alternative splicing factor ASF/SF2, interacts directly with the basic domain of Rev in vitro and that the Rev-p32 complex is resistant to high ..
  13. pmc Positive and negative modulation of human immunodeficiency virus type 1 Rev function by cis and trans regulators of viral RNA splicing
    Jodi Pongoski
    Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada
    J Virol 76:5108-20. 2002
    ....
  14. pmc Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production
    Sandrine Jacquenet
    Laboratoire de Médecine et Thérapeutique Moléculaire, INSERM CIC9501, 15 rue du Bois de la Champelle, 54500 Vandoeuvre les Nancy, France
    Retrovirology 2:33. 2005
    ..and virion production, we used a human cell line expressing high levels of complete HIV-1 and either one of the ASF/SF2, SC35, and 9G8 SR proteins...
  15. pmc Processive phosphorylation of alternative splicing factor/splicing factor 2
    Brandon E Aubol
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0506, USA
    Proc Natl Acad Sci U S A 100:12601-6. 2003
    ..out kinetic experiments specially developed to investigate how SRPK1 phosphorylates the model human SR protein, ASF/SF2...
  16. pmc The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities
    R Tacke
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    EMBO J 14:3540-51. 1995
    ASF/SF2 and SC35 belong to a highly conserved family of nuclear proteins that are both essential for splicing of pre-mRNA in vitro and are able to influence selection of alternative splice sites...
  17. ncbi A serine kinase regulates intracellular localization of splicing factors in the cell cycle
    J F Gui
    Division of Cellular and Molecular Medicine, University of California at San Diego 92093 0651
    Nature 369:678-82. 1994
    ..Our results indicate that SRPK1 may have a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells, and the reorganization of nuclear speckles during mitosis...
  18. ncbi Inactivation of the SR protein splicing factor ASF/SF2 results in genomic instability
    Xialu Li
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Cell 122:365-78. 2005
    ..Here we provide evidence that a prototypical SR protein, ASF/SF2, is unexpectedly required for maintenance of genomic stability...
  19. pmc Loss of splicing factor ASF/SF2 induces G2 cell cycle arrest and apoptosis, but inhibits internucleosomal DNA fragmentation
    Xialu Li
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 19:2705-14. 2005
    ASF/SF2 is an SR protein splicing factor that participates in constitutive and alternative pre-mRNA splicing and is essential for cell viability...
  20. pmc The gene encoding the splicing factor SF2/ASF is a proto-oncogene
    Rotem Karni
    Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York 11724, USA
    Nat Struct Mol Biol 14:185-93. 2007
    ..that the splicing factor SF2/ASF is upregulated in various human tumors, in part due to amplification of its gene, SFRS1. Moreover, slight overexpression of SF2/ASF is sufficient to transform immortal rodent fibroblasts, which form ..
  21. pmc Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF
    Rahul Sinha
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell Biol 30:2762-74. 2010
    ..The SR protein SF2/ASF is an oncoprotein that functions in pre-mRNA splicing, with additional roles in other posttranscriptional and ..
  22. pmc A splicing-independent function of SF2/ASF in microRNA processing
    Han Wu
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27708, USA
    Mol Cell 38:67-77. 2010
    ..we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF...
  23. doi The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression
    Teresa Ezponda
    Department of Pathology, Clinica Universidad de Navarra, Pamplona, Spain
    Clin Cancer Res 16:4113-25. 2010
    SF2/ASF is a splicing factor recently described as an oncoprotein...
  24. pmc SRSF1 regulates the alternative splicing of caspase 9 via a novel intronic splicing enhancer affecting the chemotherapeutic sensitivity of non-small cell lung cancer cells
    Jacqueline C Shultz
    Department of Biochemistry, Virginia Commonwealth University School of Medicine, Richmond, VA 23298 0614, USA
    Mol Cancer Res 9:889-900. 2011
    ..Specifically, the pre-mRNA sequence of caspase 9 was analyzed for RNA cis-elements known to interact with SRSF1, a required enhancer for caspase 9 RNA splicing...
  25. pmc Oncogenic splicing factor SRSF1 is a critical transcriptional target of MYC
    Shipra Das
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell Rep 1:110-7. 2012
    The SR protein splicing factor SRSF1 is a potent proto-oncogene that is frequently upregulated in cancer. Here, we show that SRSF1 is a direct target of the transcription factor oncoprotein MYC...
  26. pmc Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence
    Oliver I Fregoso
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 50:56-66. 2013
    ..Therefore, the cell has many safeguards against such misregulation. We report that the oncogenic splicing factor SRSF1, which is overexpressed in many cancers, stabilizes the tumor suppressor protein p53 by abrogating its MDM2-..
  27. ncbi Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators
    H Ge
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Cell 66:373-82. 1991
    We described previously the purification of a human protein, called alternative splicing factor (ASF), that can switch utilization of alternative 5' splice sites in an SV40 early pre-mRNA...
  28. ncbi Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
    J D Kohtz
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Nature 368:119-24. 1994
    ..Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA...
  29. ncbi Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
    J Y Wu
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 75:1061-70. 1993
    ..We report that the splicing factors SC35 and SF2/ASF specifically interact with both the integral U1 small nuclear ribonucleoprotein (snRNP U1-70K) and with the 35 ..
  30. ncbi SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors
    K Colwill
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    J Biol Chem 271:24569-75. 1996
    ..two kinases phosphorylate the same SR proteins in vitro, but that SRPK1 has the higher specific activity toward ASF/SF2. SRPK1, like Clk/Sty, phosphorylates ASF/SF2 in vitro on sites that are also phosphorylated in vivo...
  31. pmc A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
    J F Caceres
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 2208, USA
    Genes Dev 12:55-66. 1998
    ..We show here that whereas some human SR proteins are confined to the nucleus, three of them-SF2/ASF, SRp20, and 9G8-shuttle rapidly and continuously between the nucleus and the cytoplasm...
  32. pmc SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
    H Y Wang
    Division of Cellular and Molecular Medicine, Department of Medicine, University of California, San Diego, La Jolla, California 92093 0651, USA
    J Cell Biol 140:737-50. 1998
    ..Phosphorylation of an SR protein (ASF/SF2) by either SRPK1 or 2 enhanced its interaction with another RS domain-containing protein (U1 70K), and ..
  33. ncbi A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding
    W Cao
    Department of Pharmacology and Cancer Biology, Levine Science Research Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:20629-35. 1998
    ..ASF/SF2, SC35, and other members of the serine/arginine family, interact with the 70k protein of the U1 small nuclear ..
  34. pmc Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2
    S H Xiao
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    EMBO J 17:6359-67. 1998
    ..We reported previously that phosphorylation of the SR protein ASF/SF2 enhances its interaction with the U1 snRNP-specific 70K protein and is required for the protein to function in ..
  35. ncbi The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs)
    J Koizumi
    Department of Functional Genomics, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113, Japan
    J Biol Chem 274:11125-31. 1999
    ..The C-terminal arginine-serine domain of these proteins, such as SF2/ASF, mediates protein-protein interactions and is phosphorylated in vivo...
  36. pmc Transportin-SR, a nuclear import receptor for SR proteins
    N Kataoka
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Department of Biochemistry and Biophysics, Philadelphia, Pennsylvania 19104 6148, USA
    J Cell Biol 145:1145-52. 1999
    ..SR protein import receptor, termed transportin-SR (TRN-SR), binds specifically and directly to the RS domains of ASF/SF2 and SC35 as well as several other SR proteins...
  37. ncbi A human importin-beta family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins
    M C Lai
    Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei 11529, Taiwan
    J Biol Chem 275:7950-7. 2000
    ..Thus, our results likely reflect a role of TRN-SR2 in the cellular trafficking of phosphorylated SR proteins...
  38. pmc Identification and characterization of a novel serine-arginine-rich splicing regulatory protein
    D C Barnard
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Mol Cell Biol 20:3049-57. 2000
    ..even inhibit the in vitro and in vivo activation of specific splice sites by a subset of SR proteins, including ASF/SF2, SC35, and SRp55. In contrast, p86 activates splicing in the presence of SRp20...
  39. pmc Nuclear export and retention signals in the RS domain of SR proteins
    Demian Cazalla
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland
    Mol Cell Biol 22:6871-82. 2002
    ..We analyzed mutant versions of human SF2/ASF in which the natural RS repeats were replaced by RD or RE repeats and compared the splicing and subcellular ..
  40. ncbi Transcriptional activators differ in their abilities to control alternative splicing
    Guadalupe Nogues
    Laboratorio de Fisiologia y Biologia Molecular, Departamento de Fisiologia, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, C1428EHA Buenos Aires, Argentina
    J Biol Chem 277:43110-4. 2002
    ..Rapid, highly processive transcription favors EDI exon skipping, whereas slower, less processive transcription favors inclusion...
  41. pmc Exonic splicing enhancer-dependent selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site can be rescued in a cell lacking splicing factor ASF/SF2 through activation of the phosphatidylinositol 3-kinase/Akt pathway
    Xuefeng Liu
    HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:2105-15. 2003
    ..Two exonic splicing enhancers, each containing two ASF/SF2 (alternative splicing factor/splicing factor 2) binding sites, are located between the two 3' splice sites and have been identified as regulating ..
  42. ncbi Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A
    Hiroshi Umehara
    Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto 606 8502, Japan
    Biochem Biophys Res Commun 301:324-9. 2003
    ..The yeast two-hybrid system revealed that CROP interacted with SF2/ASF, an SR protein involved in RNA splicing, as well as CROP itself...
  43. ncbi SR splicing factors serve as adapter proteins for TAP-dependent mRNA export
    Yingqun Huang
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA
    Mol Cell 11:837-43. 2003
    ..Thus, multiple adapters including SR proteins most likely cooperate to recruit multiple copies of TAP/NXF1 for efficient mRNA export...
  44. ncbi Differential effects of the SR proteins 9G8, SC35, ASF/SF2, and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA
    Delphine Ropers
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, UMR CNRS 7567, Universite Henri Poincare Nancy 1, Boulevard des Aiguillettes, BP239, 54506 Vandoeuvre les Nancy, France
    J Biol Chem 279:29963-73. 2004
    ..b>ASF/SF2 strongly activates site A2 and to a lesser extent site A1...
  45. pmc A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression
    Massimo Caputi
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Virol 78:6517-26. 2004
    ..The ESE is localized downstream of the vpu-, env-, and nef-specific 3' splice site no. 5. SF2/ASF and SRp40 activate the ESE and are required for efficient 3' splice site usage and binding of the U1 snRNP to the ..
  46. ncbi Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins
    Ming Chih Lai
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    J Biol Chem 279:31745-9. 2004
    ..receptor TAP associates preferentially with the hypophosphorylated form of shuttling SR proteins, including ASF/SF2. Overexpression of ASF induced subnuclear relocalization of TAP to SR protein-enriched nuclear speckles, suggesting ..
  47. pmc SF2/ASF binds the human papillomavirus type 16 late RNA control element and is regulated during differentiation of virus-infected epithelial cells
    Maria G McPhillips
    Institute of Biomedical and Life Sciences, Division of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 78:10598-605. 2004
    ..Here, we show that the SR protein SF2/ASF is part of a complex that forms upon the 79-nucleotide negative regulatory element (NRE) that is thought to be ..
  48. pmc A novel SR-related protein is required for the second step of Pre-mRNA splicing
    Demian Cazalla
    MRC Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh EH4 2XU, Scotland, United Kingdom
    Mol Cell Biol 25:2969-80. 2005
    ..SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing...
  49. ncbi Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene
    Claudia Ghigna
    Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy
    Mol Cell 20:881-90. 2005
    ..parallels the relative abundance of DeltaRon mRNA and depends on a sequence directly bound by splicing factor SF2/ASF...
  50. ncbi SRp30a (ASF/SF2) regulates the alternative splicing of caspase-9 pre-mRNA and is required for ceramide-responsiveness
    Autumn Massiello
    Department of Biochemistry, Virginia Commonwealth University, Richmond, 23298, USA
    J Lipid Res 47:892-7. 2006
    ..The downregulation of SRp30a in A549 cells by RNA interference technology resulted in an increase in the caspase-9b splice variant, with a ..
  51. ncbi Unproductive splicing of SR genes associated with highly conserved and ultraconserved DNA elements
    Liana F Lareau
    Department of Molecular and Cell Biology and, University of California, Berkeley, California 94720, USA
    Nature 446:926-9. 2007
    ..We find that unproductive splicing associated with conserved regions has arisen independently in different SR genes, suggesting that splicing factors may readily acquire this form of regulation...
  52. pmc Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing
    Stephanie D Shaw
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS ONE 2:e854. 2007
    ..However, we previously reported that the RS domain of the SR protein SF2/ASF is dispensable for in vitro splicing of some pre-mRNAs...
  53. pmc A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1
    Jacky Chi Ki Ngo
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Cell 29:563-76. 2008
    The 2.9 A crystal structure of the core SRPK1:ASF/SF2 complex reveals that the N-terminal half of the basic RS domain of ASF/SF2, which is destined to be phosphorylated, is bound to an acidic docking groove of SRPK1 distal to the active ..
  54. doi The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1
    Gracjan Michlewski
    Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Mol Cell 30:179-89. 2008
    The SR protein SF2/ASF has been initially characterized as a splicing factor but has also been shown to mediate postsplicing activities such as mRNA export and translation...
  55. pmc The splicing-factor oncoprotein SF2/ASF activates mTORC1
    Rotem Karni
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 105:15323-7. 2008
    The splicing factor SF2/ASF is an oncoprotein that is up-regulated in many cancers and can transform immortal rodent fibroblasts when slightly overexpressed...
  56. pmc Identification of nuclear and cytoplasmic mRNA targets for the shuttling protein SF2/ASF
    Jeremy R Sanford
    MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    PLoS ONE 3:e3369. 2008
    ..that ectopic expression of SF2/ASF regulates the splicing and polysome association of transcripts derived from the SFRS1, PABC1, NETO2 and ENSA genes...
  57. pmc Human papillomavirus type 16 E2 protein transcriptionally activates the promoter of a key cellular splicing factor, SF2/ASF
    Sarah Mole
    Room 312, Jarrett Building, Institute of Comparative Medicine, University of Glasgow, Garscube Estate, Glasgow G61 1QH, Scotland, UK
    J Virol 83:357-67. 2009
    ..Previously, we demonstrated that upon epithelial differentiation, HPV16 infection upregulates hnRNP A1 and SF2/ASF, both key factors in alternative splicing regulation...
  58. pmc Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts
    Jeremy R Sanford
    Department of Molecular, Cellular, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064, USA
    Genome Res 19:381-94. 2009
    ..we define the specificity of a central regulator of pre-mRNA splicing, the conserved, essential splicing factor SFRS1. Cross-linking immunoprecipitation and high-throughput sequencing (CLIP-seq) identified 23,632 binding sites for ..
  59. pmc Chromatin binding of SRp20 and ASF/SF2 and dissociation from mitotic chromosomes is modulated by histone H3 serine 10 phosphorylation
    Rebecca J Loomis
    Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
    Mol Cell 33:450-61. 2009
    ..We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate with interphase chromatin, are released from hyperphosphorylated mitotic chromosomes, but reassociate ..
  60. doi SF2/ASF regulates proteomic diversity by affecting the balance between translation initiation mechanisms
    Matias Blaustein
    Instituto de Fisiologia, Biología Molecular y Neurociencias, Buenos Aires, Argentina
    J Cell Biochem 107:826-33. 2009
    ..have been described for a subset of shuttling serine/arginine-rich splicing regulatory proteins, among them SF2/ASF...
  61. pmc The SR protein family
    Peter J Shepard
    Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697 4025, USA
    Genome Biol 10:242. 2009
    ..These activities point up the importance of SR proteins during the regulation of mRNA metabolism...
  62. pmc SF2/ASF autoregulation involves multiple layers of post-transcriptional and translational control
    Shuying Sun
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Struct Mol Biol 17:306-12. 2010
    ..Splicing factor, arginine/serine-rich 1 (SFRS1), the gene encoding SF2/ASF, is a potent proto-oncogene with abnormal expression in many tumors...
  63. pmc Multiple ASF/SF2 sites in the human papillomavirus type 16 (HPV-16) E4-coding region promote splicing to the most commonly used 3'-splice site on the HPV-16 genome
    Monika Somberg
    Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, Sweden
    J Virol 84:8219-30. 2010
    ..human papillomavirus type 16 (HPV-16) mRNAs expressing the viral oncogenes E6 and E7 are regulated by cellular ASF/SF2, itself defined as a proto-oncogene and overexpressed in cervical cancer cells...
  64. pmc The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation
    Vidisha Tripathi
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Mol Cell 39:925-38. 2010
    ..Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression...
  65. pmc Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF)
    Dongxu Sun
    Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Nat Immunol 12:853-60. 2011
    ..other mRNAs through a pathway that involved the adaptor Act1, the adaptors TRAF2 or TRAF5 and the splicing factor SF2 (also known as alternative splicing factor (ASF))...
  66. pmc The splicing factor SRSF1 regulates apoptosis and proliferation to promote mammary epithelial cell transformation
    Olga Anczukow
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Struct Mol Biol 19:220-8. 2012
    The splicing-factor oncoprotein SRSF1 (also known as SF2/ASF or ASF/SF2) is upregulated in breast cancers. We investigated the ability of SRSF1 to transform human and mouse mammary epithelial cells in vivo and in vitro...
  67. pmc Splicing factor SF2/ASF rescues IL-2 production in T cells from systemic lupus erythematosus patients by activating IL-2 transcription
    Vaishali R Moulton
    Division of Rheumatology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:1845-50. 2013
    ..We recently showed that the serine arginine protein splicing factor 2/alternative splicing factor (SF2/ASF) enhances the expression of CD3ζ chain by limiting the production of ..
  68. ncbi SR proteins: a conserved family of pre-mRNA splicing factors
    A M Zahler
    Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington 98104
    Genes Dev 6:837-47. 1992
    ..In four of the family members a repeated protein sequence that encompasses an RNA recognition motif was observed. We discuss the potential role of this highly conserved, functionally related set of proteins in pre-mRNA splicing...
  69. ncbi Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
    A R Krainer
    Cold Spring Harbor Laboratory, New York 11724 2208
    Cell 66:383-94. 1991
    b>SF2 is a protein factor essential for constitutive pre-mRNA splicing in HeLa cell extracts and also activates proximal alternative 5' splice sites in a concentration-dependent manner...
  70. pmc The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution
    K Colwill
    Programme in Molecular Biology adn Cancer, Mount Sinai Hospital, Toronto, Canada
    EMBO J 15:265-75. 1996
    ..In vitro, Clk/Sty efficiently phosphorylated the SR family member ASF/SF2 on serine residues located within its serine/arginine-rich region (the RS domain)...
  71. ncbi A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA
    H Tronchere
    Department and School of Medicine, University of California at San Diego, La Jolla 92093 0651, USA
    Nature 388:397-400. 1997
    ..Therefore, Urp and U2AF35 may independently position RS-domain-containing factors within spliceosomes...
  72. ncbi Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins
    J Zhu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 8:1351-61. 2001
    ..We investigated how ESS3 in HIV-1 tat exon 3 blocks splicing promoted by one SR protein (SC35) but not another (SF2/ASF)...
  73. ncbi Involvement of SR proteins in mRNA surveillance
    Zuo Zhang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Mol Cell 16:597-607. 2004
    ..Our data argue against a universal role of PTCs in regulating pre-mRNA splicing and reveal an additional function of SR proteins in eukaryotic gene expression...
  74. ncbi CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing
    Hiroshi Maita
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Exp Cell Res 303:375-87. 2005
    ..and studied their subcellular localizations and interactions with PAP-1 and other SR proteins, including SC35, SF2/ASF, and U2AF35...
  75. pmc An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine
    T Lewis
    Plum Island Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Greenport, New York 11944 0848, USA
    J Virol 74:1275-85. 2000
    ..All pigs previously infected with Delta9GL survived infection when subsequently challenged with a lethal dose of virulent parental MAL. Thus, ASFV 9GL gene deletion mutants may prove useful as live-attenuated ASF vaccines.
  76. ncbi Radiosensitivity of mesothelioma cell lines
    A M Häkkinen
    Department of Oncology, University Central Hospital, Helsinki, Finland
    Acta Oncol 35:451-6. 1996
    ..The survival fraction after a dose of 2 Gy (SF2), parameters alpha and beta of the linear quadratic model (LQ-model) and mean inactivation dose (D(MID)) were also ..
  77. ncbi [Influence of angiotensin on the secretion of aldosterone in idiopathic hyperaldosteronism]
    M Salvador
    , , Toulouse
    Arch Mal Coeur Vaiss 80:992-4. 1987
    ..an increase of adrenal sensitivity to angiotensin II infusions, and isolated an aldosterone-stimulating factor (ASF). Plasma aldosterone levels were related to increased ASF concentrations but there was no link between PRA and ASF...
  78. ncbi Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5
    T Maeda
    Department of Radiology and Radiation Oncology, Osaka University School of Dentistry, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    Carcinogenesis 20:2341-4. 1999
    ..Gene expression of three splicing regulatory factors, alternative splicing factor/splicing factor 2 (ASF/SF2), heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) and the 65 kDa subunit of U2 small nuclear ..
  79. pmc An RNA splicing enhancer-like sequence is a component of a splicing inhibitor element from Rous sarcoma virus
    L M McNally
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Cell Biol 18:3103-11. 1998
    ..The second is a purine-rich region in the 5' half that interacts with the splicing factor SF2/ASF. In this study we investigated the possibility that this purine-rich region provides an RNA splicing enhancer ..
  80. doi Increased expression of cellular RNA-binding proteins in HPV-induced neoplasia and cervical cancer
    Joanna Fay
    School of Biological Sciences, Dublin Institute of Technology, Dublin, Ireland
    J Med Virol 81:897-907. 2009
    ..of a panel of RNA-binding proteins (heterogeneous ribonucleoprotein (hnRNP) A1, hnRNP C1/C2, hnRNP H, hnRNP I, ASF/SF2, SR proteins, HuR and U2AF(65)) and markers of differentiation, proliferation and neoplasia (cytokeratin (CK) 13, ..
  81. ncbi Interaction between ionizing radiation, estrogens and antiestrogens in the modification of tumor microenvironment in estrogen dependent multicellular spheroids
    M Villalobos
    Department of Radiology, School of Medicine, University of Granada, Spain
    Acta Oncol 34:413-7. 1995
    ..arrested in an estrogen-free medium showed a significant decrease in radiosensitivity (surviving fraction at 2 Gy, SF2 = 63%) when compared with 0.1 nM 17 beta-estradiol-treated cells (SF2 = 38%)...
  82. ncbi Effect of blocking TNF on IL-6 levels and metastasis in a B16-BL6 melanoma/mouse model
    S Cubillos
    Laboratorio de Fisiopatologia, Instituto Venezolano de Investigaciones Cientificas (IVIC, Caracas, Venezuela
    Anticancer Res 17:2207-11. 1997
    ..Serum IL-6 levels were increased in inoculated animals. Injection of a hybrid molecule (p55-sf2) composed of the human p55 TNF receptor extracellular domain coupled to a human constant region backbone, decreased ..
  83. ncbi Prospective pulmonary function evaluation following open thoracotomy for anterior spinal fusion in adolescent idiopathic scoliosis
    E J Graham
    Washington University, Department of Orthopaedic Surgery, St Louis, MO, USA
    Spine (Phila Pa 1976) 25:2319-25. 2000
    ..sequential pulmonary function tests (PFTs) at a minimum 2-year follow-up after an open anterior spinal fusion (ASF) with instrumentation for thoracic AIS...
  84. doi Assessment of interactions between African swine fever virus, bushpigs (Potamochoerus larvatus), Ornithodoros ticks and domestic pigs in north-western Madagascar
    J Ravaomanana
    FOFIFA, Malagasy Centre for Research in Agronomy, Antananarivo, Madagascar
    Transbound Emerg Dis 58:247-54. 2011
    Since its introduction in Madagascar in 1998, African swine fever (ASF) has severely affected national pig production and persists as a common disease in that country...
  85. ncbi [Potentials of DNA-PKcs, Ku80, and ATM in enhancing radiosensitivity of cervical carcinoma cells]
    Liang Zhuang
    Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P R China
    Ai Zheng 26:724-9. 2007
    ..This study tried to explore the correlations of DNA-PKcs, Ku80, and ATM expression to radiosensitivity of cervical cancer cells, and to probe their potentials in enhancing the radiosensitivity of cervical carcinoma...
  86. ncbi Single-dose and fractionated irradiation of four human lung cancer cell lines in vitro
    O Brodin
    Department of Oncology, Akademiska sjukhuset, University of Uppsala, Sweden
    Acta Oncol 30:967-74. 1991
    ..22 to 1.65. The surviving fraction after 2 Gy (SF2) was 0.24 and 0.42 respectively in the SCLC cell lines and 0.90 and 0.88 respectively in the NSCLC cell lines...
  87. ncbi Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2
    T B Deb
    Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 271:2206-12. 1996
    ..has complete homology with the cDNA sequence of a protein P-32, co-purified with the human pre-mRNA splicing factor SF2 (Krainer, A.R., Mayeda, A., Kozak, D., and Binns, G. (1991) Cell 66, 383-394)...
  88. ncbi Mathematical modelling of survival of glioblastoma patients suggests a role for radiotherapy dose escalation and predicts poorer outcome after delay to start treatment
    N G Burnet
    University of Cambridge, Department of Oncology, Oncology Centre, Addenbrooke s Hospital, UK
    Clin Oncol (R Coll Radiol) 18:93-103. 2006
    ....
  89. ncbi Apical uptake of organic cations by human intestinal Caco-2 cells: putative involvement of ASF transporters
    F Martel
    Department of Biochemistry, Faculty of Medicine, Porto, Portugal
    Naunyn Schmiedebergs Arch Pharmacol 363:40-9. 2001
    ..organic cations, by testing the possibility of involvement of known members of the amphiphilic solute facilitator (ASF) family in this process...
  90. doi Disturbed expression of type 1 iodothyronine deiodinase splice variants in human renal cancer
    Agnieszka Piekielko-Witkowska
    Department of Biochemistry and Molecular Biology, The Medical Center of Postgraduate Education, 01 813 Warsaw, Poland
    Thyroid 19:1105-13. 2009
    ..The expression of DIO1 is disturbed in different types of cancer. The aim of the study was to analyze the alternative splicing of DIO1 and its possible disturbance in renal cancer...
  91. ncbi Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study
    N B Arweiler
    Department of Periodontology and Conservative Dentistry, University of the Saarland, Homburg, Germany
    J Clin Periodontol 28:168-74. 2001
    ..of this study was to evaluate the clinical and antibacterial properties of alcohol-free mouthrinses, an amine fluoride/stannous fluoride mouthrinse (ASF) and two triclosan solutions in comparison with a chlorhexidine and a placebo rinse.
  92. pmc SRp20 and CUG-BP1 modulate insulin receptor exon 11 alternative splicing
    Supriya Sen
    VA San Diego Healthcare System, San Diego, California 92161, USA
    Mol Cell Biol 29:871-80. 2009
    ..We identified binding of SRp20 and SF2/ASF to the exonic enhancers and CUG-BP1 to the exonic silencer by RNA affinity chromatography...
  93. ncbi Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses
    Pedro J Sánchez-Cordón
    Departamento de Anatomia y Anatomia Patologica Comparadas, Facultad de Veterinaria, Universidad de Cordoba, Edificio de Sanidad Animal, Campus de Rabanales, 14014, Cordoba, Spain
    Am J Vet Res 68:772-7. 2007
    ..serum concentrations of the selected acute-phase proteins (APPs) haptoglobin, serum amyloid A (SAA), and C-reactive protein (CRP) in pigs experimentally inoculated with classical swine fever (CSF) and African swine fever (ASF) viruses.
  94. ncbi Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: implications for pre-mRNA processing
    Laura K Zerbe
    Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, Colorado 802262, USA
    Mol Carcinog 41:187-96. 2004
    ..Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and alternative splicing factor/splicing factor 2 (ASF/SF2) act antagonistically to modulate splice site selection...
  95. pmc The persistence of African swine fever virus in field-infected Ornithodoros erraticus during the ASF endemic period in Portugal
    Fernando S Boinas
    Centro de Investigação Interdisciplinar em Medicina Veterinária, Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa, Lisboa, Portugal
    PLoS ONE 6:e20383. 2011
    African swine fever (ASF) is an important disease of pigs and outbreaks of ASF have occurred in Europe on multiple occasions...
  96. pmc Reconstitution of ionic channels from inner and outer membranes of mammalian cardiac nuclei
    E Rousseau
    Department of Physiology and Biophysics, University of Sherbrooke, Quebec J1H 5N4, Canada
    Biophys J 70:703-14. 1996
    ..The lamins, which are associated with the inner nuclear membrane, were present in three fractions, SF2, SF3, and SF4, with a lower amount in SF2...
  97. pmc hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc
    David M Mauger
    Box 3053 424 CARL, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
    Mol Cell Biol 28:5403-19. 2008
    ..that an exonic GGG motif overlapped a critical exonic splicing enhancer, which was predicted to bind the SR protein ASF/SF2...
  98. pmc Gait in thoracolumbar/lumbar adolescent idiopathic scoliosis: effect of surgery on gait mechanisms
    Philippe Mahaudens
    Rehabilitation and Physical Medicine Unit, Universite Catholique de Louvain, Unite de Readaptation, Tour Pasteur 5375, Avenue Mounier 53, 1200, Brussels, Belgium
    Eur Spine J 19:1179-88. 2010
    ..The use of anterior spinal fusion (ASF) instead of the classic posterior fusion (PSF) may preserve more distal spinal levels in attempt to limit the ..
  99. ncbi Glutathione reduces the toxicity associated with antitumor therapy of ascites fluid adsorbed over Staphylococcus aureus Cowan I in tumor bearing mice
    A S Verma
    Industrial Toxicology Research Centre, Lucknow, India
    Toxicol Lett 106:119-27. 1999
    ..p.) on alternate days for 15 days with adsorbed ascites fluid (ad-ASF) (0.1 ml) and glutathione (GSH) (250 mg/kg body weight) separately...
  100. ncbi hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing
    Tsuyoshi Kashima
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Hum Mol Genet 16:3149-59. 2007
    ..has been attributed to loss of an exonic splicing enhancer (ESE) dependent on the SR protein splicing factor ASF/SF2 or to the creation of an exonic splicing silencer (ESS) element that functions by binding of the splicing repressor ..
  101. pmc Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF
    Zhiqi Chen
    Transplant Research Division, Department of Surgery and Immunology, The Toronto Hospital, University of Toronto, Toronto, Canada
    Nucleic Acids Res 38:6684-96. 2010
    ..splicing enhancer (ESE) located in exon 2, which is a putative binding site for a splicing regulatory protein SF2/ASF. Deletion or mutation of the ESE site decreased expression of the full-length CD200...

Research Grants62

  1. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
    ..For example, the gene encoding the splicing factor SF2/ASF is a proto-oncogene that can be activated by gene amplification in breast cancer...
  2. Alternative Splicing of the Insulin Receptor Gene
    NICHOLAS J WEBSTER; Fiscal Year: 2013
    ..Specific Aim #2: To determine whether SRp20 or SF2 is required for transcriptional pausing and co- transcriptional splicing of the INSR gene...
  3. Ilker Kudret Sariyer; Fiscal Year: 2016
    ..We recently identified the alternative splicing factor, SF2/ASF, as a potential regulator of JCV as its overexpression in glial cells strongly suppresses viral gene expression and ..
  4. VAISHALI MOULTON; Fiscal Year: 2016
    ..these proteins, a promising candidate is the serine arginine (SR) family member Alternative Splicing Factor/Splicing Factor 2 (ASF/SF2)...
  5. ASF/SF2: Phosphorylation and RNA Binding
    Gourisankar Ghosh; Fiscal Year: 2012
    Alternating splicing factor/splicing factor 2 (ASF/SF2), a member of the serine-arginine (SR) protein family, plays important roles both in constitutive and alternative pre-mRNA splicing...
  6. Regulation of tumorigenic spliced isoforms in cancer
    Aixa Tapia Santos; Fiscal Year: 2010
    ..Mutations affecting sequences specific for the binding of splicing factors SC35 and SF2/ASF in exon 11 result in changes in the MDM2 alternative spliced isoform, both in normal and in stress conditions...
  7. Gross Hall Animal Resource Center
    Susan V Bryant; Fiscal Year: 2010
    ..requests funds to build-out and equip 23,385 gross square feet (GSF) of shell space at the UCI to create the 17,433 ASF Gross Hall Animal Resource Center (GHARC) to support PHS-funded researchers...
  8. James L Manley; Fiscal Year: 2015
    ..An ASF/SF2-CTD fusion protein has been used to show that the CTD can enhance splicing in vitro in the presence of a ..
  9. Splicing factors as therapeutic targets for the treatment of ovarian cancer
    William T Beck; Fiscal Year: 2012
    ..Indeed, several splicing factors, including PTB and SRp20, are overexpressed in tumors and one, SF2/ASF, was shown to be an oncogene...
  10. BLANTON SMITH TOLBERT; Fiscal Year: 2016
    ..to identify the molecular principles that HIV uses to co-opt the mutually antagonistic factors, hnRNP A1 (A1) and ASF, in order to regulate its 3'splice site A7 (ssA7)...
  11. Mechanism of APOBEC3-Mediated Innate Immunity to HIV-1
    Yong Hui Zheng; Fiscal Year: 2012
    ..This subfamily includes ASA,A3B, A3C, A3DE, ASF, A3G and ASH, which belong to the cytidine deaminase family...
  12. ALAN KENNETH PERCY; Fiscal Year: 2016
    ..have active affiliation with the International Rett Syndrome Foundation (IRSF), the Angelman Syndrome Foundation (ASF), and the Prader-Willi Syndrome Association (PWSA). A website for this RDCRC is available at www.circ.uab.edu...
  13. Sphingolipid Regulation of Caspase 9 Alternative Splicing
    Charles Chalfant; Fiscal Year: 2009
    ..laboratory disclose that the alternative splicing of caspase 9 is intrinsically linked to the SR protein, SRp30a (ASF/SF2)...
  14. Role of APOL1 in HIVAN pathogenesis
    Waldemar Popik; Fiscal Year: 2013
    ..Renal pathogenic SF2 Nef and mutants will be used to discern mechanism(s) by which SF2 Nef inhibits autophagy and how APOL1 WT overcomes ..
  15. The Alport Syndrome Symposium for Physicians, Researchers and Families
    Clifford E Kashtan; Fiscal Year: 2010
    ..and Outcomes Registry (ASTOR), located at the University of Minnesota, and the Alport Syndrome Foundation (ASF)...
  16. HUMAN CYTOMEGALOVIRUS TEGUMENT PROTEINS
    Thomas E Shenk; Fiscal Year: 2013
    ..that it is required for efficient replication in epithelial cells, and we have shown that it binds to the cellular SF2/ASF protein, a multi-functional splicing factor...
  17. Gregory J Phillips; Fiscal Year: 2016
    ..simplified mouse model whose GI tract is colonized with only eight bacterial species, the altered Schaedler flora (ASF). Collectively, the ASF facilitates normal physiological function and health of the mouse GI system...
  18. VACCINE TO INDUCE NEUTRALIZATION OF PRIMARY HIV STRAINS
    David Davis; Fiscal Year: 1999
    ..A sensitive assay will be developed for monitoring neutralizing and enhancing antibody against the homologous HIV-1 SF2 and heterologous primary clade B viruses (HIV-1 DH12, HIV-1 HAN2, for which stocks titrated in chimps exist) in ..
  19. Rodent Holding for WIMR Cardiovascular Research
    Richard L Moss; Fiscal Year: 2013
    ..We seek to outfit three currently unequipped animal housing rooms (total 696 assignable square feet, ASF) with high-density caging and to properly equip the two adjacent procedure rooms (total 222 ASF)...
  20. Mechanism of SR Protein Binding to the Splicing Kinase SRPK1
    RYAN MATTHEW PLOCINIK; Fiscal Year: 2011
    ..Recent kinetic and crystallographic studies indicate that SRPK1 phosphorylates the RS domain of the SR protein, ASF/SF2, using a mechanism that is directional, regiospecific, and processive...
  21. Validation of a Predictive Model of Clinical Response to Concurrent Radiochemothe
    Javier F Torres Roca; Fiscal Year: 2010
    ..The main clinical endpoint is pathological response. The translational endpoint is predicted radiosensitivity (SF2)...
  22. PAR04-122, Extramural Research Facilities Improvement P*
    Michael R Grever; Fiscal Year: 2010
    ..The specific renovation project being proposed is for the "buildout" of a floor of the BRT (approximately 24,000 asf) to centralize and support one of the fastest growing and developing areas of cancer research, the Experimental ..
  23. Mouse Caging System for the University of California Davis
    KC KENT LLOYD; Fiscal Year: 2010
    ..In anticipation of an award, UC Davis has provided a dedicated, 20,000 ASF laboratory building, including a 6000 sq ft, 5-room mouse vivarium, for mouse-related research and resource ..
  24. REGULATION OF ALTERNATIVE PROCESSING OF CALCITONIN/CGRP
    Susan Berget; Fiscal Year: 2002
    ..the enhancer has a CORE resembling a pseudo exon and binds splicing factors, including U1 snRNPs, SR proteins (ASF/SF2 and SRp20), and PTB...
  25. Cardiovascular and Regenerative Sciences in WIMR Phase II
    ROBERT NEAL GOLDEN; Fiscal Year: 2010
    ..we will fully build-out and equip one floor of WIMR II, providing more than 25,000 assignable square feet (ASF) of highly integrated space...
  26. Nanospheres as Vehicles for Treatment of Neuroblastoma
    Thomas Shea; Fiscal Year: 2004
    ..In our ongoing studies we have developed an anti-oxidant synergistic formulation ("ASF"), comprised of alpha-tocopherol (vitamin E), sodium pyruvate and phosphatidyl choline, that supports axonal ..
  27. Role of ATM in Radiosensitivity of Gliomas
    Chandan Guha; Fiscal Year: 2004
    ..In-vitro, the surviving fraction after 2 Gy (SF2) decreased, from >0.5 without antisense to 0.28-0.35 with antisense...
  28. NEUROENDOCRINE REGULATION OF ENDOCRINE GLANDS
    NOELLE GRANGER; Fiscal Year: 1992
    ..factor (JH III ATF), which specifically stimulates the synthesis of JH III, and a JH I allatostatic factor (JH I ASF) which inhibits the synthesis of JH I...
  29. RR-03-011, Extramural Research Facilities Construction *
    Dallas Hyde; Fiscal Year: 2004
    ..The building will include 5,992 assignable square feet (ASF) of laboratory research space, 178 ASF of research support space and 1,200 ASF of office space...
  30. EXTRAMURAL RESEARCH FACILITIES CONSTRUCTION
    RIGHARD KRUGMAN; Fiscal Year: 2004
    This application requests $3,221,187 of matching funds to finish 31,000 asf/45,400 gsf on the second and third floors of the Davis building...
  31. Rederivation and Internal Quarantine Suite (RIQS)
    JEFFREY GOODWIN; Fiscal Year: 2009
    ..provided by applicant): This animal resource improvement application requests funds to renovate and equip 2,332 ASF of existing vivarium and procedure space at the University of California, Irvine (UCI) to create the Rederivation &..
  32. Regulation of Polyadenylation: the U1A/U1snRNP Paradigm
    Samuel Gunderson; Fiscal Year: 2007
    ....
  33. Novel Assays to Identify HIV Vif Inhibitors
    Dana Gabuzda; Fiscal Year: 2006
    ..Vif protein is required during virus replication to overcome the antiviral activity of APOBEC3G (A3G) and APOBEC3F (ASF), cytidine deaminases that induce G to A hypermutation in newly synthesized viral DNA...
  34. STAT FACTOR AND SIGNAL TRANSDUCTION AND CANCER
    Xin Yuan Fu; Fiscal Year: 2000
    ..Manley's laboratory at Columbia University, where he was the first to identify the alternative splicing factor, ASF. When he was a postdoctoral fellow with Dr...
  35. Design of molecules that promote SMN2 exon 7 inclusion
    ADRIAN KRAINER; Fiscal Year: 2005
    ..An SF2/ASF-dependent exonic splicing enhancer in exon 7 appears to be defective in SMN2, and recognition of this exon is ..
  36. AUTOSTEREO FIBERSCOPE FOR 3-D MONITORING OF TUMORS
    WINSTON HO; Fiscal Year: 1993
    ..even more accurate diagnoses, Physical Optics Corporation (POC) proposes to develop an autostereo fiberscope (ASF) for real-time 3-D monitoring of cancerous tumors, in vivo, without the need for open surgery...
  37. Formation of Heterochromatin and Cellular Senescence Driven by HIRA and ASF 1a
    Gregory Enders; Fiscal Year: 2009
    ..Specific Aim 3. Investigate the role of GSK3 activity in localization of HIRA to PML bodies, formation of SAHF and onset of senescence. ..
  38. Microecology-murine gut-initiation & progression of IBD
    James Fox; Fiscal Year: 2005
    ..A standardized microbiota used in colonizing germfree rodents referred to "Altered Schaedler Flora" (ASF) was developed...
  39. FMRI FACILITY FOR THE BRAIN IMAGING CENTER
    David Bailey; Fiscal Year: 1999
    ..through gifts, campus discretionary and loan funds to support construction of a 4,390 assignable square foot (ASF) facility as an addition to the School's Basic Science Building...
  40. A Mouse Model for Schimke Immuno-osseous Dysplasia
    Cornelius Boerkoel; Fiscal Year: 2006
    ..associated, actin dependent regulator of chromatin, subfamily a-like 1), which encodes a protein with homology to SF2 helicases and SNF2 chromatin remodeling proteins. SMARCAL1 resides in both the nucleus and the cytoplasm...
  41. Identification and analysis of substrate activators of the Aurora B kinase
    JILL SCHUMACHER; Fiscal Year: 2009
    ..elegans cell cycle we will: 1) Determine the functional relationship between TLK-1, ASF-1, ICP-1, and AIR-2 in chromosome behavior, 2) Define the molecular differences between the TLK-1/ICP-1/AIR-2 ..
  42. IL13 EFFECTS ON MURINE HIV1 GP120 T CELL RESPONSES
    Luis Montaner; Fiscal Year: 1999
    ..To do this, the investigators will immunize mice with bovine RNAase or gp120 SF2 in the presence or absence of IL-13, and test the T cell responses against both RNAase, which has known early and ..
  43. Ventilated Mouse Cages for NIH Funded Construction Projects
    DENNIS CUNNINGHAM; Fiscal Year: 2006
    ..Neuroscience Research Facility and central to recent state-of-the-art laboratory construction, including two 50,000 ASF laboratory buildings (Sprague and Hewitt Hall), the requested 2,268 mouse cages, installed in a combined holding ..
  44. SPLICING ENHANCER REGULATION OF GROWTH HORMONE ISOFORMS
    Fritz Rottman; Fiscal Year: 1999
    ..the precise nucleotide sequence of the bGH exonic enhancer, and the role of important functional domains of SF2/ASF (RNA binding and Arg/Ser domains) will be defined. 2...
  45. EXTRAMURAL FACILITIES IMPROVEMENT PROGRAM
    Robert Golden; Fiscal Year: 2004
    ..matching support from its discretionary resources, the University of Wisconsin Medical School will construct 24,336 ASF of space devoted to translational cancer research in a new Interdisciplinary Research Complex (IRC)...
  46. FACILITIES CONSTRUCTION CENTERALIZED PHARMCOGENOMICS
    Palmer Taylor; Fiscal Year: 2003
    In this rewritten and resubmitted application, funds are requested to match State of California funds for an 8,059 ASF Centralized Pharmacogenomics and Toxicogenomics Animal Facility in a new pharmaceutical sciences building...
  47. Structural Plasticity of an HIV-1 Nef/Pak-2 Activity Surface
    J Victor Garcia Martinez; Fiscal Year: 2007
    ..in all available databases and translate our observations by generating mutated versions of the prototypical SF2 Nef that reflect naturally occurring sequences...
  48. EXTRAMURAL RESEARCH FACILITIES CONSTRUCTION
    MARTIN CADWALLADER; Fiscal Year: 2004
    ..8) Purchase of equipment: primate caging and cage washer Completion of this project (6,061 ASF) will enable efficient expansion and greater proximity of PHS supported research-using NHP, particularly in areas ..
  49. Genomic analysis of RNA binding protein target specificity
    JEREMY ROBERT SANFORD; Fiscal Year: 2010
    ..2) Determine the genomic landscape of cis-acting RNA elements recognized by SR proteins. (3) Functionally validate SR protein cis-acting RNA elements. ..
  50. Nucleic Acid Gordon Research Conference
    ANNA PYLE; Fiscal Year: 2004
    ..Poster sessions and optional recreational activities will stimulate informal interactions among participants. ..
  51. How does DHEA improve diabetic microvascular dysfunction
    JOSEPH DILLON; Fiscal Year: 2004
    ..Thus, the successful completion of our project will advance our long-term goal of developing novel therapeutic vasoactive agents, to decrease the morbidity related to diabetic vascular dysfunction. ..
  52. DEHYDROEPIANDROSTERONE SULFATE AND INSULIN SECRETION
    JOSEPH DILLON; Fiscal Year: 2004
    ..4) Assess the effects of DHEAS on ACS activity, glucose-stimulated insulin secretion and ACO mRNA expression in human pancreatic islets. ..
  53. Peptide Vaccine for Experimental Autoimmune Myocarditis
    Daniel Zimmerman; Fiscal Year: 2003
    ..The effects of J-My-1 administration on the presence and frequency of this cell type will be evaluated. Our goal is to demonstrate that the severity of EAM can be reduced by J-My-1 administration in animals with EAM. ..
  54. derG Immunostimulant Prevention/Treatment of HSV Disease
    Daniel Zimmerman; Fiscal Year: 2003
    ..An integral part of the phase II program will be to further elucidate the target cells and mechanism of action of derG. ..
  55. REGULATION OF THE STIMULATION OF MUCIN GENE EXPRESSION A
    Ja Seok Koo; Fiscal Year: 2003
    ....
  56. HIV Vaccine Development
    David Montefiori; Fiscal Year: 2003
    ....
  57. CONFERENCE ON DNA REPLICATION AND RECOMBINATION
    KENNETH MARIANS; Fiscal Year: 2002
    ..Given the rapid pace of progress in these fields over the last three years, it should prove to be a timely and exciting meeting. ..
  58. Role of Integrins in Pulmonary Fibrosis
    Eric White; Fiscal Year: 2006
    ....
  59. Microbial Production Core Facility
    Wolf Dietrich Heyer; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  60. Enteric flora modulates the development of colitis
    Albert Jergens; Fiscal Year: 2008
    ..g., graduate students and postdoctoral fellow). There are ample opportunities to attend seminars, journal clubs and work-in-progress research meetings during which the applicant can become engaged in scientific discussions. ..
  61. RED CELL CYTOSKELETAL PROTEIN AND MITOSIS
    Shu Ching Huang; Fiscal Year: 2008
    ..Finally, we shall define the relationship between changes in the expression of these 4.1R isoforms and the transition from proliferation to post-mitotic states during erythroid differentiation. ..
  62. Young Adult Environmental and Physical Activity Dynamics
    Barry Popkin; Fiscal Year: 2008
    ..No study heretofore has had large-scale groupings and in-depth environmental measures over time to examine these issues in a dynamic manner. ..