En1

Summary

Gene Symbol: En1
Description: engrailed 1
Alias: En-1, Mo-en.1, engrailed-1, homeobox protein engrailed-1, homeobox protein en-1, mo-En-1
Species: mouse

Top Publications

  1. ncbi Expression patterns of the homeo box-containing genes En-1 and En-2 and the proto-oncogene int-1 diverge during mouse development
    C A Davis
    Division of Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada
    Genes Dev 2:1736-44. 1988
  2. ncbi The mouse Engrailed-1 gene and ventral limb patterning
    C A Loomis
    Ronald O Perelman Deparment of Dermatology, New York University Medical School 10016, USA
    Nature 382:360-3. 1996
  3. ncbi Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs
    C A Loomis
    Ronald O Perelman Department of Dermatology, NYU Medical School, New York, NY 10016, USA
    Development 125:1137-48. 1998
  4. pmc Identification of Dlk1, Ptpru and Klhl1 as novel Nurr1 target genes in meso-diencephalic dopamine neurons
    Frank M J Jacobs
    Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Development 136:2363-73. 2009
  5. ncbi Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1
    Mark Zervas
    Howard Hughes Medical Institute, Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Neuron 43:345-57. 2004
  6. ncbi En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis
    A L Joyner
    Department of Anatomy, University of California at San Francisco 94143
    Genes Dev 1:29-38. 1987
  7. ncbi V1 spinal neurons regulate the speed of vertebrate locomotor outputs
    Simon Gosgnach
    Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 440:215-9. 2006
  8. ncbi Requirement for Wnt3 in vertebrate axis formation
    P Liu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 22:361-5. 1999
  9. ncbi Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3
    Marten P Smidt
    Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, University Medical Center, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Development 131:1145-55. 2004
  10. ncbi The midbrain-hindbrain phenotype of Wnt-1-/Wnt-1- mice results from stepwise deletion of engrailed-expressing cells by 9.5 days postcoitum
    A P McMahon
    Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Cell 69:581-95. 1992

Scientific Experts

Detail Information

Publications152 found, 100 shown here

  1. ncbi Expression patterns of the homeo box-containing genes En-1 and En-2 and the proto-oncogene int-1 diverge during mouse development
    C A Davis
    Division of Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada
    Genes Dev 2:1736-44. 1988
    ..Later in development the En genes may have an additional function in neurogenesis. En-1 expression in the developing pericordal tube suggests that it may also be involved in vertebral assembly...
  2. ncbi The mouse Engrailed-1 gene and ventral limb patterning
    C A Loomis
    Ronald O Perelman Deparment of Dermatology, New York University Medical School 10016, USA
    Nature 382:360-3. 1996
    ..Engrailed-1 seems to act in part by repressing dorsal differentiation induced by Wnt-7a, and is essential for proper formation of the apical ectodermal ridge...
  3. ncbi Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs
    C A Loomis
    Ronald O Perelman Department of Dermatology, NYU Medical School, New York, NY 10016, USA
    Development 125:1137-48. 1998
    ..We have previously shown that the limbs of En1 mutant mice display dorsal-ventral and proximal-distal abnormalities, the latter being reflected in the appearance ..
  4. pmc Identification of Dlk1, Ptpru and Klhl1 as novel Nurr1 target genes in meso-diencephalic dopamine neurons
    Frank M J Jacobs
    Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Development 136:2363-73. 2009
    ....
  5. ncbi Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1
    Mark Zervas
    Howard Hughes Medical Institute, Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Neuron 43:345-57. 2004
    ..Finally, we also uncovered transient and differential genetic lineages of ventral midbrain dopaminergic and ventral hindbrain serotonergic neuronal precursors with respect to Wnt1 and Gli1 expression...
  6. ncbi En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis
    A L Joyner
    Department of Anatomy, University of California at San Francisco 94143
    Genes Dev 1:29-38. 1987
    ..Both genes map in the vicinity of mutations that are known to cause abnormalities during development...
  7. ncbi V1 spinal neurons regulate the speed of vertebrate locomotor outputs
    Simon Gosgnach
    Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 440:215-9. 2006
    ....
  8. ncbi Requirement for Wnt3 in vertebrate axis formation
    P Liu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 22:361-5. 1999
    ..These studies provide genetic proof for the requirement of Wnt3 in primary axis formation in the mouse...
  9. ncbi Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3
    Marten P Smidt
    Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, University Medical Center, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Development 131:1145-55. 2004
    ..However, an overall lower activity was observed. The results suggest that Pitx3 is specifically required for the formation of the SNc subfield at the onset of dopaminergic neuron differentiation...
  10. ncbi The midbrain-hindbrain phenotype of Wnt-1-/Wnt-1- mice results from stepwise deletion of engrailed-expressing cells by 9.5 days postcoitum
    A P McMahon
    Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Cell 69:581-95. 1992
    ..We suggest that functional redundancy between these two genes accounts for the lack of a caudal central nervous system phenotype...
  11. ncbi Fibroblast growth factor-8 expression is regulated by intronic engrailed and Pbx1-binding sites
    J Gemel
    Department of Pediatrics and Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:6020-6. 1999
    ..In addition, in vitro mutagenesis of both Engrailed and Pbx1 sites indicated that other unidentified sites are responsible for the transcriptional enhancement observed with the intronic fragment...
  12. ncbi Ectopic engrailed 1 expression in the dorsal midline causes cell death, abnormal differentiation of circumventricular organs and errors in axonal pathfinding
    A Louvi
    CNRS UMR 8542, Equipe Régionalisation nerveuse, Ecole Normale Superieure, 75230 Paris Cedex 05, France
    Development 127:4061-71. 2000
    ..In WEXPZ.En1 transgenic mice (Danielian, P. S. and McMahon, A. P...
  13. ncbi Requirement for LIM homeobox gene Isl1 in motor neuron generation reveals a motor neuron-dependent step in interneuron differentiation
    S L Pfaff
    Howard Hughes Medical Institute, Department of Biochemistry and Molecule Biophysics, Columbia University, New York, New York 10032, USA
    Cell 84:309-20. 1996
    ..A population of interneurons that express Engrailed1 (EN1), however, also fails to differentiate in Isl1 mutant embryos...
  14. ncbi Protease nexin-1 is expressed at the mouse met-/mesencephalic junction and FGF signaling regulates its promoter activity in primary met-/mesencephalic cells
    P Kury
    Friedrich Miescher Institut, Basel, Switzerland
    Development 124:1251-62. 1997
    ....
  15. ncbi Control of cell pattern in the neural tube by the zinc finger transcription factor and oncogene Gli-1
    M Hynes
    Department of Neuroscience, Genentech, Inc, South San Francisco, California 94080, USA
    Neuron 19:15-26. 1997
    ..These findings demonstrate that GLI-1 can reproduce the cell patterning actions of Shh in the developing nervous system and provide support for the hypothesis that it is a mediator of the Shh signal in vertebrates...
  16. ncbi Nurr1 regulates dopamine synthesis and storage in MN9D dopamine cells
    Elisabet Hermanson
    Ludwig Institute for Cancer Research, Stockholm Branch, P O Box 240, S 171 77 Stockholm, Sweden
    Exp Cell Res 288:324-34. 2003
    ..Together, the results provide evidence indicating an instructive role for Nurr1 in controlling DA synthesis and storage...
  17. pmc Pax6 and engrailed 1 regulate two distinct aspects of renshaw cell development
    Tamar Sapir
    Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    J Neurosci 24:1255-64. 2004
    ..Pax6 (paired box gene 6) is necessary for an early step in Renshaw cell development, whereas Engrailed 1 (En1), which is genetically downstream of Pax6, regulates the formation of inhibitory synapses between Renshaw ..
  18. ncbi Growth and pattern of the mammalian neural tube are governed by partially overlapping feedback activities of the hedgehog antagonists patched 1 and Hhip1
    Juhee Jeong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 132:143-54. 2005
    ..Furthermore, this feedback mechanism is crucial in generating a neural tube that contains appropriate numbers of all ventral and intermediate neuronal cell types...
  19. ncbi Mitochondrial fusion protects against neurodegeneration in the cerebellum
    Hsiuchen Chen
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 130:548-62. 2007
    ....
  20. ncbi PAX2 is expressed in multiple spinal cord interneurons, including a population of EN1+ interneurons that require PAX6 for their development
    J D Burrill
    Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
    Development 124:4493-503. 1997
    ..of PAX2-expressing interneurons in the spinal cord are marked by coexpression of the transcription factors, EN1 and EVX1...
  21. ncbi The loss of ventral ectoderm identity correlates with the inability to form an AER in the legless hindlimb bud
    S M Bell
    Division of Developmental Biology Children s Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Mech Dev 74:41-50. 1998
    ..These data suggest that establishment of a dorso-ventral ectodermal interface is not sufficient for AER formation and that restriction of lmx-1b to the dorsal mesenchyme is coordinately linked to AER formation...
  22. ncbi Manifestation of the limb prepattern: limb development in the absence of sonic hedgehog function
    C Chiang
    Department of Cell Biology, Vanderbilt University Medical Center, 1161 21st Avenue South, Nashville, Tennessee 37232, USA
    Dev Biol 236:421-35. 2001
    ..According to this model, the limb bud signaling centers, including the zone of polarizing activity (ZPA) acting through Shh, are required to elaborate upon the axial information provided by the native limb field prepattern...
  23. ncbi The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum
    Candace L Chi
    Department of Anatomy and Program in Developmental Biology, School of Medicine, University of California at San Francisco, San Francisco, CA 94143 0452, USA
    Development 130:2633-44. 2003
    ..remarkably similar pattern of cell death in Wnt1 null homozygotes, and also detected ectopic mes/met cell death in En1 null homozygotes...
  24. ncbi Location and size of dopaminergic and serotonergic cell populations are controlled by the position of the midbrain-hindbrain organizer
    Claude Brodski
    Max Planck Institute of Psychiatry, 80804 Munich, Germany, and GSF National Research Center for Environment and Health, Technical University Munich, Institute of Developmental Genetics, 85758 Oberschleissheim, Germany
    J Neurosci 23:4199-207. 2003
    ..In addition, our data suggest that the position of the MHO during embryogenesis can modulate adult locomotor activity...
  25. ncbi Lmx1b is essential for Fgf8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice
    Chao Guo
    Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Development 134:317-25. 2007
    ..Moreover, transcription factors En1 and Pax2 were also downregulated prior to the 4-somite stage, whereas Gbx2 downregulation occurred at the 4-somite ..
  26. pmc Notch and MAML signaling drives Scl-dependent interneuron diversity in the spinal cord
    Chian Yu Peng
    Department of Neurobiology, University of Chicago, Chicago, IL 60637, USA
    Neuron 53:813-27. 2007
    ..Thus, our study provides insight into the cell-extrinsic signaling that controls combinatorial transcription factor profiles involved in regulating the process of interneuron subtype diversification...
  27. doi Anterior-posterior graded response to Otx2 controls proliferation and differentiation of dopaminergic progenitors in the ventral mesencephalon
    Daniela Omodei
    CEINGE Biotecnologie Avanzate, Via Comunale Margherita 482, 80145 Naples, Italy
    Development 135:3459-70. 2008
    ..Thus, our data provide new insights into the mechanism of mesDA neuron specification and suggest Otx2 as a potential target for cell replacement-based therapeutic approaches in PD...
  28. pmc Wnt5a regulates ventral midbrain morphogenesis and the development of A9-A10 dopaminergic cells in vivo
    Emma R Andersson
    Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 3:e3517. 2008
    ....
  29. doi Dynamic expression and heterogeneous intracellular location of En-1 during late mouse embryonic development
    Shan Chuan Zhong
    Department of Histology and Embryology, Third Military Medical University, Chongqing, Republic of China
    Cells Tissues Organs 191:289-300. 2010
    ..These findings provided additional evidence that En-1 may be involved in the development of neural crest cells...
  30. doi Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP
    Michela Di Salvio
    CEINGE Biotecnologie Avanzate, Naples, Italy
    Nat Neurosci 13:1481-8. 2010
    ..These findings indicate that Otx2 is required to specify neuron subtype identity in VTA and may antagonize vulnerability to the Parkinsonian toxin MPTP...
  31. doi Retinoic acid-dependent and -independent gene-regulatory pathways of Pitx3 in meso-diencephalic dopaminergic neurons
    Frank M J Jacobs
    Rudolf Magnus Institute, Department of Neuroscience and Pharmacology, UMC Utrecht, Utrecht, The Netherlands
    Development 138:5213-22. 2011
    ..RA signaling represents only one aspect of the Pitx3 downstream cascade, as Vmat2, Dat, Ahd2 (Aldh1a1), En1, En2 and Cck were unaffected by RA treatment and are (subset) specifically modulated by Pitx3...
  32. pmc Dynamic temporal requirement of Wnt1 in midbrain dopamine neuron development
    Jasmine Yang
    Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, 70 Ship Street, Providence, RI 02903, USA
    Development 140:1342-52. 2013
    ..Interestingly, an En1-derived domain persists after the early deletion of Wnt1 and mutant cells express OTX2...
  33. ncbi Requirement for Lim1 in head-organizer function
    W Shawlot
    Department of Molecular Genetics, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nature 374:425-30. 1995
    ..A partial secondary axis developed anteriorly in some mutant embryos. Lim1 is thus an essential regulator of the vertebrate head organizer...
  34. ncbi Rostral optic tectum acquires caudal characteristics following ectopic engrailed expression
    C Logan
    Department of Developmental Neurobiology, UMDS, Guy s Hospital, London SE1 9RT, UK
    Curr Biol 6:1006-14. 1996
    ....
  35. ncbi nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
    I Varlet
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 124:1033-44. 1997
    ..Thus we conclude that the action of nodal, a TGFbeta-related growth factor expressed in the primitive endoderm, is critical for patterning of the anterior aspects of the A-P axis...
  36. ncbi Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons
    M P Matise
    New York University Medical School, Skirball Institute of Biomolecular Medicine, Developmental Genetics Program, New York, New York 10016, USA
    J Neurosci 17:7805-16. 1997
    ..Rather, it is suggested that En-1 may function to distinguish a subset of interneurons during the later maturation of the spinal cord...
  37. ncbi Genetic control of brain morphogenesis through Otx gene dosage requirement
    D Acampora
    International Institute of Genetics and Biophysics, CNR, Naples, Italy
    Development 124:3639-50. 1997
    ....
  38. ncbi Cripto is required for correct orientation of the anterior-posterior axis in the mouse embryo
    J Ding
    Center for Advanced Biotechnology and Medicine and Dept of Pediatrics, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Nature 395:702-7. 1998
    ..Our results indicate that Cripto signalling is essential for the conversion of a proximal-distal asymmetry into an orthogonal anterior-posterior axis...
  39. ncbi Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation
    D Acampora
    International Institute of Genetics and Biophysics, CNR, Via G Marconi 12, Italy
    Development 125:5091-104. 1998
    ..Moreover, our data lead us to hypothesize that the differential post-transcriptional control existing between VE and epiblast cells may potentially contribute to fundamental regulatory mechanisms required for head specification...
  40. ncbi A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord
    A Pierani
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Cell 97:903-15. 1999
    ..analysis of the induction of ventral progenitors that express the Dbx homeodomain proteins and of Evx1/2 (V0) and En1 (V1) neurons...
  41. pmc Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation
    R A Kimmel
    Department of Cell Biology, New York University School of Medicine, New York, New York 10016 USA
    Genes Dev 14:1377-89. 2000
    ..One border is transient and at the limit of expression of the ventral gene En1, which corresponds to the D/V midline of the AER, and the second border corresponds to the dorsal AER margin...
  42. ncbi Some distal limb structures develop in mice lacking Sonic hedgehog signaling
    P Kraus
    Ronald O Perelman Department of Dermatology, New York University School of Medicine, NY, New York, USA
    Mech Dev 100:45-58. 2001
    ..Finally, we show that Shh is also required for outgrowth of the limb ectoderm and thus for the formation of a distinct limb compartment...
  43. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  44. ncbi Fate of midbrain dopaminergic neurons controlled by the engrailed genes
    H H Simon
    Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    J Neurosci 21:3126-34. 2001
    ..However, the engrailed genes control the survival of midbrain dopaminergic neurons in a gene dose-dependent manner. Our findings also suggest a link between engrailed and Parkinson's disease...
  45. ncbi Distinct regulators control the expression of the mid-hindbrain organizer signal FGF8
    W Ye
    Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA
    Nat Neurosci 4:1175-81. 2001
    ..A network of transcription and secreted factors, including En1, Otx2, Gbx2, Grg4 and Wnt1&4, that is established independently of Pax2, further refines the expression domain ..
  46. ncbi BMPR-IA signaling is required for the formation of the apical ectodermal ridge and dorsal-ventral patterning of the limb
    K Ahn
    Department of Neuroscience, University of Pennsylvania Medical School, Philadelphia, PA, USA
    Development 128:4449-61. 2001
    ..Along the dorsal/ventral axis, loss of engrailed 1 (En1) expression in the non-ridge ectoderm of the mutants resulted in a dorsal transformation of the ventral ..
  47. ncbi Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid/hindbrain organizer
    James Y H Li
    Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
    Neuron 36:31-43. 2002
    ..Our work has uncovered distinct requirements for Gbx2 during cerebellum formation and provided a model for how a transcription factor can play multiple roles during development...
  48. pmc Ectodermal Wnt3/beta-catenin signaling is required for the establishment and maintenance of the apical ectodermal ridge
    Jeffery R Barrow
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 17:394-409. 2003
    ..Finally, we demonstrate that Wnt/beta-catenin signaling lies upstream of the Bmp signaling pathway in establishment of the AER and regulation of the dorsoventral polarity of the limb...
  49. pmc Genetic interaction between Wnt/beta-catenin and BMP receptor signaling during formation of the AER and the dorsal-ventral axis in the limb
    Natalia Soshnikova
    Max Delbrück Centrum for Molecular Medicine, Robert Rossle Strasse 10, 13125Berlin, Germany
    Genes Dev 17:1963-8. 2003
    ..Thus, AER formation and dorsal-ventral patterning of limbs are tightly controlled by an intricate interplay between Wnt/beta-catenin and BMP receptor signaling...
  50. pmc mBtd is required to maintain signaling during murine limb development
    Dieter Treichel
    MPI für Biophysikalische Chemie, Abt Molekulare Zellbiologie, Am Fassberg, 37077 Gottingen, Germany
    Genes Dev 17:2630-5. 2003
    ..The data indicate that mBtd represents a novel key player mediating proximodistal outgrowth of the limb...
  51. ncbi Effects of Wnt1 signaling on proliferation in the developing mid-/hindbrain region
    Markus Panhuysen
    Institute of Developmental Genetics, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany
    Mol Cell Neurosci 26:101-11. 2004
    ..into Wnt1 function in the mid-/hindbrain region, we ectopically expressed Wnt1 under the control of the endogenous En1 promoter, thereby extending Wnt1 expression rostrally into the anterior midbrain and caudally into rhombomere 1...
  52. ncbi Bmp4 in limb bud mesoderm regulates digit pattern by controlling AER development
    Jennifer Selever
    Alkek Institute of Biosciences and Technology, Texas A and M System Health Science Center, Houston, TX 77030, USA
    Dev Biol 276:268-79. 2004
    ..Our data show that Bmp4 in limb mesoderm regulates AER induction and maturation and implicate signaling from the AER in regulation of digit number and identity...
  53. ncbi Engrailed genes control developmental fate of serotonergic and noradrenergic neurons in mid- and hindbrain in a gene dose-dependent manner
    Horst H Simon
    Interdisciplinary Center for Neuroscience, Department of Neuroanatomy, University of Heidelberg, 69120 Heidelberg, Germany
    Mol Cell Neurosci 28:96-105. 2005
    ..An almost identical phenotype is found in mutant mice null for Wnt1, indicating that the engrailed genes provide essential positional information for the development of the two nuclei during early embryogenesis...
  54. ncbi Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping
    Sema K Sgaier
    Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Neuron 45:27-40. 2005
    ..Thus, we show that progressive changes in the axes of the cerebellum underlie its genesis...
  55. ncbi Expression of Fgf receptors 1, 2, and 3 in the developing mid- and hindbrain of the mouse
    Alexandra A Blak
    GSF National Research Center for Environment and Health, Institute of Developmental Genetics, Neuherberg, Germany
    Dev Dyn 233:1023-30. 2005
    ..Fgfr3 expression is in contact with the Fgf8 expression domain only in the rostroventral hindbrain. Based on these findings, we postulate a role for FGFR2 and FGFR3 in FGF signaling in the ventral midbrain and hindbrain...
  56. ncbi Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors
    Rob Machold
    Department of Cell Biology, The Skirball Institute of Biomolecular Medicine, New York University Medical Center, 540 First Avenue, New York, New York 10016, USA
    Neuron 48:17-24. 2005
    ..Thus, we demonstrate that the cerebellar rhombic lip gives rise to multiple cell types within rhombomere 1...
  57. ncbi A Wnt1-regulated genetic network controls the identity and fate of midbrain-dopaminergic progenitors in vivo
    Nilima Prakash
    GSF National Research Center for Environment and Health, Technical University Munich, Institute of Developmental Genetics, Ingolstaedter Landstrasse 1, 85764 Munich Neuherberg, Germany
    Development 133:89-98. 2006
    ..They also suggest the Wnt1-controlled signaling pathway as a promising target for new therapeutic strategies in the treatment of Parkinson's disease...
  58. pmc Slow progressive degeneration of nigral dopaminergic neurons in postnatal Engrailed mutant mice
    Paola Sgadò
    Interdisciplinary Center for Neuroscience, Department of Neuroanatomy, Ruprecht Karls Universitat Heidelberg, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:15242-7. 2006
    ..this requirement, the cells die by apoptosis when all four alleles of the Engrailed genes are genetically ablated (En1-/-;En2-/-)...
  59. ncbi Selective expression of Bhlhb5 in subsets of early-born interneurons and late-born association neurons in the spinal cord
    Ben Liu
    The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Dev Dyn 236:829-35. 2007
    ..At late stages of development, Bhlhb5 expression is detected in a subset of late-born dorsal association interneurons that migrate into the uppermost layer of the dorsal horn...
  60. ncbi Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner
    Anna L M Ferri
    Division of Developmental Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 134:2761-9. 2007
    ..Subsequently, Foxa1 and Foxa2 regulate the expression of Nurr1 (Nr4a2) and engrailed 1 in immature neurons and the expression of aromatic l-amino acid decarboxylase and tyrosine hydroxylase in ..
  61. pmc Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development
    M Albert Basson
    Department of Anatomy and Program in Developmental Biology, University of California San Francisco, CA 94158, USA
    Development 135:889-98. 2008
    ..We suggest a molecular explanation for this phenomenon by providing evidence that FGF signaling functions to inhibit the BMP signaling that promotes roof plate development...
  62. doi Shh dependent and independent maintenance of basal midbrain
    Ariadna Perez-Balaguer
    Instituto de Neurociencias de Alicante, CSIC and Universidad Miguel Hernandez, Sant Joan d Alacant, Spain
    Mech Dev 126:301-13. 2009
    ..Based on our results and recently published data, we suggest the existence of a specific genetic pathway for the specification of the mesencephalic red nucleus. Foxa2 could be the candidate gene that might control this genetic pathway...
  63. doi Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development
    Wei Lin
    Division of Developmental Neurobiology, MRC National Institute for Medical Research, The Ridgeway, London, NW7 1AA, UK
    Dev Biol 333:386-96. 2009
    ..Our data therefore provide new insights into the specification and differentiation of mesodiencephalic dopaminergic neurons and identifies Foxa1 and Foxa2 as essential regulators in these processes...
  64. pmc Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo
    Andrea Wizenmann
    Institute of Developmental Genetics, Helmholtz Zentrum Munich, Neuherberg, Germany
    Neuron 64:355-66. 2009
    ..Collectively, our results indicate that extracellular Engrailed contributes to retinotectal mapping in vivo by modulating the sensitivity of growth cones to EphrinA...
  65. pmc beta-Catenin regulates intercellular signalling networks and cell-type specific transcription in the developing mouse midbrain-rhombomere 1 region
    Dmitri Chilov
    Institute of Biotechnology, University of Helsinki, Helsinki, Finland
    PLoS ONE 5:e10881. 2010
    ..These results highlight the role of beta-catenin in establishment of neuroectodermal signalling centers, promoting region-specific gene expression and regulation of cell fate determination...
  66. pmc Mir-17-3p controls spinal neural progenitor patterning by regulating Olig2/Irx3 cross-repressive loop
    Jun An Chen
    Departments of Pathology and Cell Biology, Neurology, and Neuroscience, Center for Motor Neuron Biology and Disease, Columbia University Medical Center, New York, NY 10032, USA
    Neuron 69:721-35. 2011
    ....
  67. pmc Spatially restricted and developmentally dynamic expression of engrailed genes in multiple cerebellar cell types
    Sandra L Wilson
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cerebellum 10:356-72. 2011
    ..As a means to understand how the En genes regulate multiple levels of cerebellum construction, we characterized En1 and En2 expression around birth and at postnatal day (P) 21 during the period when the cerebellum undergoes a ..
  68. pmc Role of adenylate cyclase 1 in retinofugal map development
    Onkar S Dhande
    Department of Neurobiology, Yale University, New Haven, Connecticut 06510, USA
    J Comp Neurol 520:1562-83. 2012
    ..These results suggest that AC1 in RGC axons mediates the development of retinotopy and eye-specific segregation in the SC and dorsal LGN...
  69. doi Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones
    Olivier Stettler
    CNRS Unité mixte de Recherche 7241 INSERM U1050, Equipe FRM, Center for Interdisciplinary Research in Biology, College de France, 11, place Marcelin Berthelot, 75005 Paris, France
    Development 139:215-24. 2012
    b>Engrailed 1 and engrailed 2 homeoprotein transcription factors (collectively Engrailed) display graded expression in the chick optic tectum where they participate in retino-tectal patterning...
  70. pmc A unilateral negative feedback loop between miR-200 microRNAs and Sox2/E2F3 controls neural progenitor cell-cycle exit and differentiation
    Changgeng Peng
    Institute of Developmental Genetics, Helmholtz Zentrum Munchen, Deutsches Forschungszentrum für Umwelt und Gesundheit, and Technische Universität München Weihenstephan, D 85764 Neuherberg, Germany
    J Neurosci 32:13292-308. 2012
    ..The lack of a negative regulation of Sox2 and E2F3 by miR-200 in conditional Dicer1 mutants (En1(+/Cre); Dicer1(flox/flox) mice) and after miR-200 knockdown in vitro leads to a strongly reduced cell-cycle exit ..
  71. ncbi Pax-2 expression in the murine neural plate precedes and encompasses the expression domains of Wnt-1 and En-1
    D H Rowitch
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mech Dev 52:3-8. 1995
    ..Pax-5 expression commences later, at the 3-somite stage. Thus, the spatial and temporal expression of Pax-2 is consistent with a possible regulatory role in the activation of Wnt-1 and En-1...
  72. ncbi Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2
    M Hanks
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt Sinai Hospital, Toronto, Canada
    Science 269:679-82. 1995
    ..This rescued all En-1 mutant defects, demonstrating that the difference between En-1 and En-2 stems from their divergent expression patterns...
  73. ncbi Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum
    W Wurst
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt Sinai Hospital, Toronto, Canada
    Development 120:2065-75. 1994
    ..The results of these studies suggest a cell autonomous role for En-1 in generation and/or survival of mid-hindbrain precursor cells and also a non-cell autonomous role in signalling normal development of the limbs and possibly sternum...
  74. ncbi Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants
    J A Cygan
    Department of Molecular and Cellular Biology, The Biolabs, Harvard University, Cambridge, Massachusetts 02138, USA
    Development 124:5021-32. 1997
    ..Unlike the normal AER, ectopic AER formation is dependent upon Wnt-7a activity, indicating that distinct genetic mechanisms may be involved in primary and secondary AER formation...
  75. ncbi Sequential roles for Otx2 in visceral endoderm and neuroectoderm for forebrain and midbrain induction and specification
    M Rhinn
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, Strasbourg, France
    Development 125:845-56. 1998
    ..Altogether, these results demonstrate that Otx2 is first required in the visceral endoderm for the induction, and subsequently in the neuroectoderm for the specification of forebrain and midbrain territories...
  76. ncbi Engrailed-1 and netrin-1 regulate axon pathfinding by association interneurons that project to motor neurons
    H Saueressig
    Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
    Development 126:4201-12. 1999
    ..By targeting the axonal reporter gene &tgr;-lacZ to the En1 locus, we show the cell-type-specific transcription factor Engrailed-1 (EN1) defines a population of association ..
  77. ncbi EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region
    A Liu
    Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Development 128:181-91. 2001
    ..5 diencephalic and midbrain explants treated with FGF8-soaked beads. By examining gene expression in En1/2 double mutant mouse embryos, we found that Fgf8, Wnt1 and Pax5 do not require the En genes for initiation of ..
  78. ncbi Evx1 is a postmitotic determinant of v0 interneuron identity in the spinal cord
    L Moran-Rivard
    Biology Graduate Program University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093, USA
    Neuron 29:385-99. 2001
    ..V1 classes of ventral interneurons are defined by expression of the homeodomain transcription factors Evx1/2 and En1, respectively...
  79. ncbi Engrailed genes are cell-autonomously required to prevent apoptosis in mesencephalic dopaminergic neurons
    Lavinia Alberi
    Department of Neuroanatomy, Interdisciplinary Center of Neuroscience, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany
    Development 131:3229-36. 2004
    ..The homeobox transcription factors engrailed 1 and engrailed 2 are expressed by this neuronal population from early in development to adulthood...
  80. ncbi En1 and Wnt7a interact with Dkk1 during limb development in the mouse
    Maja Adamska
    Department of Human Genetics, University of Michigan, Ann Arbor 48109 0618, USA
    Dev Biol 272:134-44. 2004
    ..The extra digits and expanded apical ectodermal ridge (AER) of Dkk1-deficient mice closely resemble En1 null mice...
  81. doi Characterization of Foxp2-expressing cells in the developing spinal cord
    Y Morikawa
    Department of Anatomy and Neurobiology, Wakayama Medical University, 811 1, Kimiidera, Wakayama 641 8509, Japan
    Neuroscience 162:1150-62. 2009
    ..staining for Foxp2 with some homeodomain transcription factors revealed that Foxp2-expressing neurons were Pax2(+), En1(+), Evx1(-), Chx10(-), Gata3(-), and Lhx3(-) V1 interneurons in the intermediate zone throughout the ventral spinal ..
  82. ncbi Engrailed-1 as a target of the Wnt-1 signalling pathway in vertebrate midbrain development
    P S Danielian
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 383:332-4. 1996
    ..This suggests that a key role of Wnt-1 signalling is to maintain En expression and that this aspect of the Wnt-1/engrailed interaction has been conserved from flies to mice...
  83. ncbi ErbB3 is required for normal cerebellar and cardiac development: a comparison with ErbB2-and heregulin-deficient mice
    S L Erickson
    Department of Molecular Biology, Genetech Inc, South San Francisco, California 94080, USA
    Development 124:4999-5011. 1997
    ..Numerous organs, including the stomach and pancreas also exhibited anomalous development...
  84. pmc MLL, a mammalian trithorax-group gene, functions as a transcriptional maintenance factor in morphogenesis
    B D Yu
    Howard Hughes Medical Institute, Division of Molecular Oncology, Departments of Medicine and Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 95:10632-6. 1998
    ..Thus, Mll represents an essential mechanism of transcriptional maintenance in mammalian development, which functions in multiple morphogenetic processes...
  85. ncbi Mesodermal patterning defect in mice lacking the Ste20 NCK interacting kinase (NIK)
    Y Xue
    New York University Medical Center, Skirball Institute of Biomolecular Medicine, Department of Pharmacology, NY, NY10016, USA
    Development 128:1559-72. 2001
    ....
  86. ncbi Rotatin is a novel gene required for axial rotation and left-right specification in mouse embryos
    Anja M Faisst
    Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany
    Mech Dev 113:15-28. 2002
    ..Our results show a novel key player in the genetic cascade that determines L-R specification, and suggest a causal link between this process and axial rotation...
  87. ncbi Coexpression of SCL and GATA3 in the V2 interneurons of the developing mouse spinal cord
    Emma Smith
    Institute for Molecular Bioscience, incorporating the Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia
    Dev Dyn 224:231-7. 2002
    ..These experiments revealed that SCL is expressed in a medially restricted subpopulation of GATA-3 producing cells, suggesting a possible role for this factor in the differentiation of the GATA population of V2 interneurons...
  88. pmc Sp8 is crucial for limb outgrowth and neuropore closure
    Sheila M Bell
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 100:12195-200. 2003
    ..These observations indicate that Sp8 functions downstream of Wnt3, Fgf10, and Bmpr1a in the signaling cascade that mediates AER formation...
  89. ncbi A specific requirement for PDGF-C in palate formation and PDGFR-alpha signaling
    Hao Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Genet 36:1111-6. 2004
    ..Our results also show that PDGF-C signaling is a new pathway in palatogenesis, different from, and independent of, those previously implicated...
  90. ncbi Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer
    Arthur C K Chung
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Dev Biol 293:13-24. 2006
    ..GCNF, diminishes the expression of secreted signaling molecules, Fgf8 and Wnt1, the paired box genes Pax2/5, En1/2, and homeodomain transcription factor Gbx2; all of which are essential for isthmic organizer function...
  91. pmc Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain
    Cynthia L Andoniadou
    Neural Development Unit, UCL Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK
    Development 134:1499-508. 2007
    ..However, conditional expression of Hesx1 within the AFB can rescue the forebrain defects observed in the Hesx1 mutants. The results presented here provide new insights into the function of Hesx1 in forebrain formation...
  92. ncbi R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development
    Motoko Aoki
    Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Dev Growth Differ 50:85-95. 2008
    ..This study shows that Rspo2 is critical for maintenance of the AER and for growth and patterning in limb development...
  93. pmc Redefining the serotonergic system by genetic lineage
    Patricia Jensen
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Neurosci 11:417-9. 2008
    ..We uncovered a molecular framework for the serotonergic system that, having genetic lineages as its basis, is likely to have physiological relevance and will permit access to genetically defined subtypes for manipulation...
  94. ncbi Mapping of the mouse bilirubin UDP-glucuronosyltransferase gene (Gnt-1) to chromosome 1 by restriction fragment length variations
    H Sato
    Department of Biology, Shiga University of Medical Science, Japan
    Biochem Genet 30:347-52. 1992
    ..Bilirubin and phenol UDPGTs have been suggested to be expressed by a single gene by alternative splicing in human and rat. The mouse bilirubin UDPGT gene was named Gnt-1...
  95. ncbi Dominant hemimelia and En-1 on mouse chromosome 1 are not allelic
    M Higgins
    MRC Human Genetics Unit, Edinburgh, UK
    Genet Res 60:53-60. 1992
    ..The likely gene order and recombination frequencies of these loci are: ln (5.2 +/- 0.9) Emv-17 (1.1 +/- 0.4) Dh (0.7 +/- 0.4) En-1 (3.0 +/- 0.7) Pep-3. This shows that Dh is not a mutant allele of En-1...
  96. ncbi Chromosomal location of murine and human IL-1 receptor genes
    N G Copeland
    Mammalian Genetics Laboratory, National Cancer Institute Frederick Cancer Research and Development Center, Maryland 21701
    Genomics 9:44-50. 1991
    ..The murine Il-1r1 gene has thus been separated from the IL-1 genes, which lie on murine chromosome 2...
  97. pmc Detection of homeobox genes in development and evolution
    M T Murtha
    Department of Biology, Yale University, New Haven, CT 06511
    Proc Natl Acad Sci U S A 88:10711-5. 1991
    ..Cloning of three Antennapedia-related sequences from cnidarians provides evidence of ancient roles for homeobox genes early in metazoan evolution...
  98. ncbi Antisense targeting of engrailed-1 causes abnormal axis formation in mouse embryos
    T W Sadler
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA
    Teratology 51:292-9. 1995
    ..Thus, in addition to participating in the signaling pathway for brain and limb development, En-1 appears to play a role in patterning the embryonic axis...
  99. ncbi Antisense inhibition of engrailed genes in mouse embryos reveals roles for these genes in craniofacial and neural tube development
    K A Augustine
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA
    Teratology 51:300-10. 1995
    ..abstract truncated at 250 words)..
  100. ncbi The HOX complex neighbored by the EVX gene, as well as two other homeobox-containing genes, the GBX-class and the EN-class, are located on the same chromosomes 2 and 7 in humans
    T Matsui
    Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Japan
    FEBS Lett 336:107-10. 1993
    ..Thus, it is possible that these homeobox genes were linked to each other on an ancestral genome and that the ancestral chromosome segment was duplicated during evolution...
  101. pmc Nucleotides flanking a conserved TAAT core dictate the DNA binding specificity of three murine homeodomain proteins
    K M Catron
    Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey
    Mol Cell Biol 13:2354-65. 1993
    ..Differences in DNA binding specificities observed in vitro may contribute to selective interactions of homeodomain proteins with potential binding sites in the control regions of target genes...

Research Grants4

  1. Multistep Lymphomagenesis/Tumorigenesis in Transgenic Mice: Protein Kinase Ck2
    David C Seldin; Fiscal Year: 2010
    ..Since CK2 is upregulated in all human tumors that have been studied, CK2 may be a suitable target for cancer treatment. Understanding basic CK2 biology and signaling is a necessary prelude to developing novel therapeutics against it. ..
  2. The Role of Id2 in Adult Neurogenesis
    MATTHEW HAVRDA; Fiscal Year: 2008
    ..These experiments are immediately relevant to realization of the therapeutic potential of these unique cells. [unreadable] [unreadable]..
  3. The Role of Sonic Hedgehog in telencephalic patterning
    Robert Machold; Fiscal Year: 2004
    ..This work will hopefully lead to a deeper understanding of regional specification in the developing forebrain. ..
  4. EFFECTS OF DIOXIN ON TOOTH AND MANDIBLE TRAITS IN MICE
    Larry Leamy; Fiscal Year: 2005
    ..The identification of such genes should allow us to test for potential effects of this toxicant, even at prevailing levels of exposure in human populations, on a myriad of human diseases. ..