Gjc1

Summary

Gene Symbol: Gjc1
Description: gap junction protein, gamma 1
Alias: C130009G16Rik, Cnx45, Cx45, Gja-7, Gja7, gap junction gamma-1 protein, connexin-45, gap junction alpha-7 protein, gap junction membrane channel protein alpha 7
Species: mouse

Top Publications

  1. doi A novel type of interplexiform amacrine cell in the mouse retina
    Karin Dedek
    Department of Neurobiology, University of Oldenburg, Oldenburg, Germany
    Eur J Neurosci 30:217-28. 2009
  2. ncbi Spatiotemporal transcription of connexin45 during brain development results in neuronal expression in adult mice
    S Maxeiner
    Institut fur Genetik, Abteilung fur Molekulargenetik, Rheinische Friedrich Wilhelms Universitat Bonn, Romerstrasse 164, D 53117, Bonn, Germany
    Neuroscience 119:689-700. 2003
  3. ncbi An update on connexin genes and their nomenclature in mouse and man
    Goran Sohl
    Institut fur Genetik, Universitat Bonn, Germany
    Cell Commun Adhes 10:173-80. 2003
  4. ncbi Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect
    Kiyomasa Nishii
    Department of Developmental Molecular Anatomy, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
    Cell Commun Adhes 10:365-9. 2003
  5. ncbi Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission
    Stephan Maxeiner
    Institute for Genetics, Division of Molecular Genetics, University of Bonn, 53117 Bonn, Germany
    J Neurosci 25:566-76. 2005
  6. ncbi Connexin45 mediates gap junctional coupling of bistratified ganglion cells in the mouse retina
    Timm Schubert
    Institute of Biology, University of Oldenburg, D 26111 Oldenburg, Germany
    J Comp Neurol 490:29-39. 2005
  7. ncbi Spatiotemporal distribution of Connexin45 in the olivocerebellar system
    Ruben S Van Der Giessen
    Department of Neuroscience, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    J Comp Neurol 495:173-84. 2006
  8. pmc Connexin45 is expressed in the juxtaglomerular apparatus and is involved in the regulation of renin secretion and blood pressure
    Fiona Hanner
    Department of Physiology, Zilkha Neurogenetic Institute, University of Southern California, 1501 San Pablo St, ZNI 335, Los Angeles, CA 90033, USA
    Am J Physiol Regul Integr Comp Physiol 295:R371-80. 2008
  9. pmc Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    J Neurosci 28:9769-89. 2008
  10. pmc Connexin expression in renin-producing cells
    Lisa Kurtz
    Institute of Physiology, University of Regensburg, Regensburg, Germany
    J Am Soc Nephrol 20:506-12. 2009

Research Grants

  1. Gap Junction Mediated Signaling and Vascular Responses
    Alexander M Simon; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications89

  1. doi A novel type of interplexiform amacrine cell in the mouse retina
    Karin Dedek
    Department of Neurobiology, University of Oldenburg, Oldenburg, Germany
    Eur J Neurosci 30:217-28. 2009
    ..A new type of interplexiform ON amacrine cell is described, which is strongly coupled and uses GABA but not dopamine as its neurotransmitter...
  2. ncbi Spatiotemporal transcription of connexin45 during brain development results in neuronal expression in adult mice
    S Maxeiner
    Institut fur Genetik, Abteilung fur Molekulargenetik, Rheinische Friedrich Wilhelms Universitat Bonn, Romerstrasse 164, D 53117, Bonn, Germany
    Neuroscience 119:689-700. 2003
    ....
  3. ncbi An update on connexin genes and their nomenclature in mouse and man
    Goran Sohl
    Institut fur Genetik, Universitat Bonn, Germany
    Cell Commun Adhes 10:173-80. 2003
    ..Furthermore, this short review contains an update on phenotypic correlations between connexin deficient mice and patients bearing mutations in their orthologous connexin genes...
  4. ncbi Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect
    Kiyomasa Nishii
    Department of Developmental Molecular Anatomy, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
    Cell Commun Adhes 10:365-9. 2003
    The gap junction protein connexin45-deficient (Cx45-KO) mice die shortly after the hearts begin to beat...
  5. ncbi Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission
    Stephan Maxeiner
    Institute for Genetics, Division of Molecular Genetics, University of Bonn, 53117 Bonn, Germany
    J Neurosci 25:566-76. 2005
    Connexin45 (Cx45) is known to be expressed in the retina, but its functional analysis was problematic because general deletion of Cx45 coding DNA resulted in cardiovascular defects and embryonic lethality at embryonic day 10.5...
  6. ncbi Connexin45 mediates gap junctional coupling of bistratified ganglion cells in the mouse retina
    Timm Schubert
    Institute of Biology, University of Oldenburg, D 26111 Oldenburg, Germany
    J Comp Neurol 490:29-39. 2005
    ..Connexin45 (Cx45) appears to be the major component of the gap junctional channels because tracer coupling is absent in Cx45-..
  7. ncbi Spatiotemporal distribution of Connexin45 in the olivocerebellar system
    Ruben S Van Der Giessen
    Department of Neuroscience, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    J Comp Neurol 495:173-84. 2006
    ..These morphological data suggest that Connexin45 may play a modifying role in widely distributed, coupled neurons of the olivocerebellar system, but that it is not essential for the creation of its neuronal gap junctions...
  8. pmc Connexin45 is expressed in the juxtaglomerular apparatus and is involved in the regulation of renin secretion and blood pressure
    Fiona Hanner
    Department of Physiology, Zilkha Neurogenetic Institute, University of Southern California, 1501 San Pablo St, ZNI 335, Los Angeles, CA 90033, USA
    Am J Physiol Regul Integr Comp Physiol 295:R371-80. 2008
    ..have identified several connexin isoforms in the juxtaglomerular apparatus (JGA), but the vascular connexin isoform Cx45 has not yet been studied in this region...
  9. pmc Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    J Neurosci 28:9769-89. 2008
    ..particularly in the inner plexiform layer (IPL), where the two principal neuronal connexin proteins are Cx36 and Cx45. Currently undetermined are coupling relationships between these connexins and whether both are expressed together ..
  10. pmc Connexin expression in renin-producing cells
    Lisa Kurtz
    Institute of Physiology, University of Regensburg, Regensburg, Germany
    J Am Soc Nephrol 20:506-12. 2009
    ..development, the appearance of renin-producing cells paralleled the expression of Cx40 and, to a lesser extent, Cx45 but not other Cxs...
  11. doi Substitution of connexin40 with connexin45 prevents hyperreninemia and attenuates hypertension
    Frank Schweda
    Institute of Physiology, University of Regensburg, Regensburg, Germany
    Kidney Int 75:482-9. 2009
    ..properties of Cx40, we measured renin release in mice where the coding sequence for Cx40 was replaced by that for Cx45, using the knock-in method...
  12. doi C-terminal tagging with eGFP yields new insights into expression of connexin45 but prevents rescue of embryonic lethal connexin45-deficient mice
    Julia von Maltzahn
    Institute of Genetics, Division of Molecular Genetics, University of Bonn, Bonn, Germany
    Eur J Cell Biol 88:481-94. 2009
    Connexin45 (Cx45) is a member of the connexin family which can form gap junction channels and is known to be expressed in several cell types in the embryonic as well as adult mouse including working cardiomyocytes and certain types of ..
  13. ncbi Defective vascular development in connexin 45-deficient mice
    O Kruger
    Institut fur Genetik, Abt Molekulargenetik, Universitat Bonn, Germany
    Development 127:4179-93. 2000
    In order to reveal the biological function(s) of the gap-junction protein connexin 45 (Cx45), we generated Cx45-deficient mice with targeted replacement of the Cx45-coding region with the lacZ reporter gene...
  14. ncbi Electrical conductance of mouse connexin45 gap junction channels is modulated by phosphorylation
    T A van Veen
    University Medical Center Utrecht, Department of Medical Physiology, The Netherlands
    Cardiovasc Res 46:496-510. 2000
    In this study we report about the modulation of connexin45 (Cx45) gap junction channel properties by phosphorylation of the connexin molecules through different protein kinases...
  15. ncbi Loss of connexin45 causes a cushion defect in early cardiogenesis
    M Kumai
    Department of Developmental Molecular Anatomy, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Development 127:3501-12. 2000
    ..These results indicate a requirement for gap junction channels during early cardiogenesis and hence implicate connexin45 in congenital heart diseases. http://www. biologists.com/Development/movies/dev4369.html..
  16. doi Deletion of connexin45 in mouse neurons disrupts one-trial object recognition and alters kainate-induced gamma-oscillations in the hippocampus
    A Zlomuzica
    Institute of Experimental Psychology, Heinrich Heine University Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Physiol Behav 101:245-53. 2010
    ..Novel object recognition after delays of up to 60min was impaired in neuronal Cx45 deficient mice. However, object-place recognition was not significantly different from controls...
  17. doi Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina
    Gerrit Hilgen
    Department of Neurobiology, University of Oldenburg, Germany
    J Comp Neurol 519:433-50. 2011
    In the mouse retina, connexin45 (Cx45) participates in the gap junction between ON cone bipolar cells and AII amacrine cells, which constitutes an essential element of the primary rod pathway...
  18. pmc Reciprocal expression of connexin 40 and 45 during phenotypical changes in renin-secreting cells
    Birguel Kurt
    Institute of Physiology, University of Regensburg, Regensburg, Germany
    Am J Physiol Renal Physiol 300:F743-8. 2011
    ..This study was designed to determine the relevance of the vascular gap junction protein connexin 45 (Cx45) for the control of renin expression and secretion...
  19. ncbi Immunochemical characterization of the gap junction protein connexin45 in mouse kidney and transfected human HeLa cells
    A Butterweck
    Institut für Genetik Abteilung Molekulargenetik, Universitat, Bonn, Germany
    J Membr Biol 141:247-56. 1994
    Antibodies to the gap junction protein connexin45 (Cx45) were obtained by immunizing rabbits with fusion protein consisting of glutathione S-transferase and 138 carboxy-terminal amino acids of mouse Cx45...
  20. ncbi Differential expression of the gap junction proteins connexin45, -43, -40, -31, and -26 in mouse skin
    A Butterweck
    Abt Molekulargenetik der Universität, Bonn Germany
    Eur J Cell Biol 65:152-63. 1994
    ..For this purpose, polyclonal antibodies to Cx31 and Cx45 were raised by immunizing rabbits with fusion proteins of glutathione S-transferase and carboxy-terminal peptides ..
  21. ncbi Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes
    H Hennemann
    Institut für Genetik Abt Molekulargenetik, Universität Bonn Germany
    Eur J Cell Biol 58:81-9. 1992
    ..Cx26 mRNA is much more abundant in adult mouse skin than in adult kidney and liver where Cx32 transcripts are relatively strongly expressed...
  22. doi Connexin45 contributes to global cardiovascular development by establishing myocardial impulse propagation
    Kiyomasa Nishii
    Department of Anatomy and Neurobiology, National Defense Medical College, 3 2 Namiki, Tokorozawa, Saitama 359 8513, Japan Electronic address
    Mech Dev 140:41-52. 2016
    ..Here, we examined mouse embryos with a region-specific deletion of Cx45 in the myocardium (CA-Cre; Cx45(flox/flox)) or endothelium (Tie2-Cre; Cx45(flox/flox))...
  23. doi Connexin 30 is expressed in the mouse sino-atrial node and modulates heart rate
    Daniel Gros
    Institut de Biologie du Developpement de Marseille Luminy, Universite de la Mediterranee, Marseille, France
    Cardiovasc Res 85:45-55. 2010
    ..This study aimed at characterizing expression and the functional role of the Gjb6 gene, encoding for connexin 30 (Cx30) protein, in the adult mouse heart...
  24. pmc Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice
    R J Chapman
    Institute for Membrane and Systems Biology, University of Leeds, Leeds LS2 9JT, UK
    Brain Struct Funct 218:751-65. 2013
    ..In this study, a Cx45 reporter mouse model in which the Cx45 coding sequence is substituted for enhanced green fluorescent protein (eGFP) ..
  25. pmc Connexin45 modulates the proliferation of transit-amplifying precursor cells in the mouse subventricular zone
    Konstantin Khodosevich
    Department of Clinical Neurobiology, University Hospital and German Cancer Research Center, DKFZ, Heidelberg, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 109:20107-12. 2012
    ..Thus, overexpression of Cx45 by retroviral injections increased the proliferation of Mash-1-positive transit-amplifying precursor cells in the ..
  26. doi Linoleic acid induces opening of connexin26 hemichannels through a PI3K/Akt/Ca(2+)-dependent pathway
    Vania Figueroa
    Departamento de Fisiologia, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Biochim Biophys Acta 1828:1169-79. 2013
    ..Since LA is an essential polyunsaturated fatty acid, its effect on HCs might be relevant to cell growth as well as to cellular functions of differentiated cells such as audition...
  27. pmc Fast structural responses of gap junction membrane domains to AB5 toxins
    Irina V Majoul
    Institute of Biology, Center for Structural and Cell Biology in Medicine, University of Lubeck, 23562 Lübeck, Germany
    Proc Natl Acad Sci U S A 110:E4125-33. 2013
    ..Our data demonstrate very fast dynamics (in the millisecond-to-second range) within GJ plaques, which previously were considered to be relatively stable, long-lived structures...
  28. pmc Characterization of the connexin45 carboxyl-terminal domain structure and interactions with molecular partners
    Jennifer L Kopanic
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    Biophys J 106:2184-95. 2014
    ..For example, Cx45 is predominately expressed in the specialized myocytes of the impulse generation and conduction system...
  29. pmc Gap junction-mediated death of retinal neurons is connexin and insult specific: a potential target for neuroprotection
    Abram Akopian
    Department of Biological and Vision Sciences, State University of New York College of Optometry, New York, New York 10036, Department of Physiology and Neuroscience, New York University School of Medicine, New York, New York 10016
    J Neurosci 34:10582-91. 2014
    ..deletion of the GJ subunit Cx36 increased cell survivability by ∼50% under excitotoxic condition, cell loss in Cx45 knock-out mouse retinas was similar to that seen in wild-type mice...
  30. pmc Connexin45 is expressed in vascular smooth muscle but its function remains elusive
    Volker J Schmidt
    Institut fur Physiologie, Universitat zu Lubeck, Lubeck, Germany
    PLoS ONE 7:e42287. 2012
    ..In contrast, expression and physiological functions of Cx45 is unclear although its expression has been suggested in vascular smooth muscle (VSM)...
  31. pmc Functional properties of mouse connexin30.2 expressed in the conduction system of the heart
    Maria M Kreuzberg
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, Germany
    Circ Res 96:1169-77. 2005
    Gap junction channels composed of connexin (Cx) 40, Cx43, and Cx45 proteins are known to be necessary for impulse propagation through the heart. Here, we report mouse connexin30.2 (mCx30...
  32. doi Delayed osteoblastic differentiation and bone development in Cx43 knockout mice
    Lucas Martins Chaible
    Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil
    Toxicol Pathol 39:1046-55. 2011
    ..associated with bone development, namely, osteocalcin, osteopontin, alkaline phosphatase, RUNX2, GJA1, GJC1 (Cx45), and GJA3 (Cx46)...
  33. pmc Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium
    Mingwei Bao
    Washington University School of Medicine, St Louis, MO, USA
    Channels (Austin) 5:489-99. 2011
    ..known about expression patterns and remodeling of cardiac connexin(Cx)43, little is known about the less abundant Cx45, which is required for embryonic development and viability, is downregulated in adult hearts, and is ..
  34. doi Prostaglandin E₂ maintains mouse ESC undifferentiated state through regulation of connexin31, connexin43 and connexin45 expression: involvement of glycogen synthase kinase 3β/β-catenin
    Seung Pil Yun
    Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju 500 757, Republic of Korea
    Biol Cell 104:378-96. 2012
    ..Therefore, we investigated the role of PGE₂ on Cx isoforms and maintenance of mouse ESC undifferentiated state...
  35. doi Connexin45 provides optimal atrioventricular nodal conduction in the adult mouse heart
    Marina Frank
    LIMES Institute, Molecular Genetics, University of Bonn, Bonn, Germany
    Circ Res 111:1528-38. 2012
    ..In the adult hearts of mice and humans, the expression mainly is restricted to the cardiac conduction system. Cx45 plays an essential role for development and function of the embryonic heart because general and cardiomyocyte-..
  36. pmc Connexins and steroidogenesis in mouse Leydig cells
    Dan Li
    Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada
    Can J Physiol Pharmacol 91:157-64. 2013
    ..It was concluded that connexin36, connexin43, and connexin45 are coexpressed in Leydig cells with connexins 36 and 43 contributing to gap junctions. The role of connexin45 remains to be elucidated...
  37. doi Coexpression of connexin 45 with connexin 43 decreases gap junction size
    Katharina Grikscheit
    Department of Cardiac Medicine, National Heart and Lung Institute, London, United Kingdom
    Cell Commun Adhes 15:185-93. 2008
    In the human heart, ventricular myocytes express connexin 43 (Cx43) and traces of Cx45. In congestive heart failure, Cx43 levels decrease, Cx45 levels increase and gap junction size decreases...
  38. ncbi The mouse gap junction gene connexin29 is highly expressed in sciatic nerve and regulated during brain development
    G Sohl
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, Germany
    Biol Chem 382:973-8. 2001
    ..Our expression data suggest that the new connexin gene is active in myelin-forming glial cells...
  39. pmc The role of neuronal connexins 36 and 45 in shaping spontaneous firing patterns in the developing retina
    Aaron G Blankenship
    Neurosciences Graduate Program, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 31:9998-10008. 2011
    ..During development, two connexin isoforms, connexin 36 (Cx36) and Cx45, are expressed in bipolar cells and RGCs, and therefore provide a potential substrate for coordinating network ..
  40. ncbi Novel cell lines promote the discovery of genes involved in early heart development
    E W Brunskill
    Division of Developmental Biology, Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3300, USA
    Dev Biol 235:507-20. 2001
    ..The results show that the 1H and ECL-2 cell lines can be used to discover novel genes expressed in the early cardiomyocyte...
  41. pmc Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node function and connexin 45 localization in the murine heart
    Byung Kwan Lim
    Department of Medicine, University of California San Diego, Division of Cardiology, La Jolla, California 92093 0613, USA
    J Clin Invest 118:2758-70. 2008
    ..In the cardiac-specific CAR-KO (CAR-cKO) mouse, we observed the loss of connexin 45 localization to the cell-cell junctions of the AV node but preservation of connexin 40 and 43 in contracting ..
  42. ncbi Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells
    P J Kausalya
    Institute of Molecular and Cell Biology, Epithelial Cell Biology Laboratory, Singapore
    FEBS Lett 505:92-6. 2001
    ..b>Cx45 interacts with the PDZ domains of ZO-1 and ZO-3, but not ZO-2, via a short C-terminal PDZ binding motif (SVWI)...
  43. pmc Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure
    Jian Fu Chen
    Carolina Cardiovascular Biology Center, Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 105:2111-6. 2008
    ..Together, our studies demonstrate essential roles for Dicer in cardiac contraction and indicate that miRNAs play critical roles in normal cardiac function and under pathological conditions...
  44. ncbi Null mutation of connexin43 causes slow propagation of ventricular activation in the late stages of mouse embryonic development
    D Vaidya
    Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    Circ Res 88:1196-202. 2001
    ..3 [7.8 to 8.9], and Cx43(-/-) 1.7 [1.3 to 2.1; P<0.005]) corresponding with the downregulation of Cx40. Cx40 and Cx45 mRNAs were detectable in ventricular homogenates even at 17...
  45. ncbi Mouse connexin 45: genomic cloning and exon usage
    A Jacob
    Department of Pediatrics, University of Chicago, Chicago, Illinois 60637 1470, USA
    DNA Cell Biol 20:11-9. 2001
    b>Connexin 45 is a gap junction protein that is prominent in early embryos and is widely expressed in many mature cell types. To elucidate its gene structure, expression, and regulation, we isolated mouse Cx45 genomic clones...
  46. ncbi Connexin expression in the retina
    G Sohl
    Institut fur Genetik, Abteilung fur Molekulargenetik, Universitat Bonn, Germany
    Brain Res Brain Res Rev 32:138-45. 2000
    ..Cx36 and Cx45 immunoreactive signals were found in the inner and outer plexiform layer, both of which are known to show ..
  47. ncbi Gap junctional connexins in the developing mouse cardiac conduction system
    L Miquerol
    Laboratoire de Génétique at Physiologie du Développement CNRS UMR 6545, Institut de Biologie du Developpement de Marseille, Universite de la Mediterranee, Marseille, France
    Novartis Found Symp 250:80-98; discussion 98-109, 276-9. 2003
    ..In the adult mouse heart, Cx43 is expressed in all the working myocytes and most of the conductive myocytes; Cx45 is weakly expressed in all conductive myocytes, including those of the nodal tissues; Cx40 expression is restricted ..
  48. ncbi Connexins and impulse propagation in the mouse heart
    J Jalife
    Department of Pharmacology, SUNY Health Science Center, Syracuse, New York 13210, USA
    J Cardiovasc Electrophysiol 10:1649-63. 1999
    ..Three gap junction proteins, known as connexins, are expressed in the heart: Cx40, Cx43, and Cx45. Each of these proteins forms channels with unique biophysical and electrophysiologic properties, as well as ..
  49. pmc Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins
    Francisco J del Castillo
    Unite de Genetique et Physiologie de l Audition, Institut Pasteur, Paris, France
    Hum Mol Genet 19:262-75. 2010
    ..Sequence accession numbers. GenBank: Human CNST cDNA, NM_152609; mouse Cnst cDNA, NM_146105...
  50. ncbi Downregulation of connexin 45 gene products during mouse heart development
    S Alcolea
    Laboratoire de Genetique et Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, CNRS INSERM AP Marseille Université de la Méditerranée, Campus de Luminy, Marseille
    Circ Res 84:1365-79. 1999
    ..Three members of this family have been demonstrated to be synthesized in the cardiomyocytes: Cx40, Cx43, and Cx45. In addition, each of them has been shown to form channels with unique and specific electrophysiological properties...
  51. ncbi Connexin expression and functional analysis of gap junctional communication in mouse embryonic stem cells
    Philipp Wörsdörfer
    Division of Molecular Genetics, Institute of Genetics, University of Bonn, Bonn, Germany
    Stem Cells 26:431-9. 2008
    ..purpose, we have analyzed all known connexin genes in mouse ES cells but found only three of them, Cx31, Cx43, and Cx45, to be expressed as proteins...
  52. pmc Transplacental uptake of glucose is decreased in embryonic lethal connexin26-deficient mice
    H D Gabriel
    Institut fur Genetik, Abt Molekulargenetik, Universitat Bonn, 53117 Bonn, Germany
    J Cell Biol 140:1453-61. 1998
    ....
  53. ncbi Heart defects in connexin43-deficient mice
    J Ya
    Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, The Netherlands
    Circ Res 82:360-6. 1998
    ....
  54. ncbi Expression of the gap junction proteins connexin31 and connexin43 correlates with communication compartments in extraembryonic tissues and in the gastrulating mouse embryo, respectively
    E Dahl
    Abt Molekulargenetik, Universitat Bonn, Germany
    J Cell Sci 109:191-7. 1996
    ..The communication boundary between those compartments may be due to incompatibility of connexin31 and connexin43 hemichannels, which do not communicate with each other in cell culture...
  55. ncbi Multiple members of the connexin gene family participate in preimplantation development of the mouse
    T C Davies
    Department of Zoology, University of Western Ontario, London, Canada
    Dev Genet 18:234-43. 1996
    ..They can be divided into two groups with respect to the timing of mRNA accumulation: Cx31, Cx43, and Cx45 mRNAs accumulate continuously from the two- or four-cell stage, whereas Cx30.3, Cx31...
  56. ncbi Developmental regulation of connexin 40 gene expression in mouse heart correlates with the differentiation of the conduction system
    B Delorme
    Laboratoire de Genetique et Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, Faculte des Sciences de Luminy, Universite de la Mediterranee, France
    Dev Dyn 204:358-71. 1995
    ..e., CX37, CX43, and CX45, with a decreasing abundance as development proceeds...
  57. ncbi Chromosomal assignments of mouse connexin genes, coding for gap junctional proteins, by somatic cell hybridization
    H J Schwarz
    Institut fur Genetik, Abt Molekulargenetik, Universitat Bonn, Germany
    Somat Cell Mol Genet 18:351-9. 1992
    The connexin genes Cx31 and Cx45 coding for proteins of gap junctional subunits have been assigned to mouse chromosomes 4 and 11 by Southern blot hybridization of specific gene probes to DNA from mouse x Chinese hamster somatic cell ..
  58. ncbi Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs
    H Hennemann
    Institut für Genetik Abt Molekulargenetik der Universität, Bonn Deutschland
    Eur J Cell Biol 57:51-8. 1992
    ..Cx31 and Cx45 are coded for by single genes in the mouse genome...
  59. ncbi Interplay between cystic fibrosis transmembrane regulator and gap junction channels made of connexins 45, 40, 32 and 50 expressed in oocytes
    Basilio A Kotsias
    Department of Pharmacology and Physiology, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642 8711, USA
    J Membr Biol 214:1-8. 2006
    ..functional interaction between CFTR and gap junction channels, we coexpressed in Xenopus oocytes CFTR and either Cx45, Cx40, Cx32 or Cx50 and monitored junctional conductance (G (j)) and its sensitivity to transjunctional voltage (V (..
  60. ncbi Gap junctions in the inner ear: comparison of distribution patterns in different vertebrates and assessement of connexin composition in mammals
    Andrew Forge
    UCL Centre for Auditory Research and Institute of Laryngology and Otology, University College London, London WC1X 8EE, United Kingdom
    J Comp Neurol 467:207-31. 2003
    ..Heteromeric cx26/cx30 connexons may be unique to the inner ear, which could be one factor underlying the non-syndromic character of the deafness caused by mutations in cx26...
  61. ncbi Replacement of connexin40 by connexin45 in the mouse: impact on cardiac electrical conduction
    Sébastien Alcoléa
    Laboratoire de Genetique et Physiologie du Developpement, UMR CNRS 6545, Institut de Biologie du Developpement de Marseille, Universite de la Mediterranee, Marseille, France
    Circ Res 94:100-9. 2004
    ..Cx43, Cx40, and Cx45 are synthesized in the cardiomyocytes, and each of them has a unique cardiac expression pattern...
  62. ncbi Comparison of connexin expression patterns in the developing mouse heart and human foetal heart
    Steven R Coppen
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College, Royal Brompton Hospital, London, UK
    Mol Cell Biochem 242:121-7. 2003
    ..The expression of the three major connexins (Cx43, Cx40 and Cx45) present in cardiac myocytes is known to be developmentally regulated but it is not clear how the patterns in the ..
  63. ncbi Sequence and structure of the mouse connexin45 gene
    D Baldridge
    Department of Internal Medicine, Washington University School of Medicine, Barnes Jewish Hospital, St Louis, Missouri, USA
    Biosci Rep 21:683-9. 2001
    The connexin45 (Cx45) gene was cloned from a mouse genomic Bacterial Artificial Chromosome library. Approximately 8.4 kb of the genomic DNA was sequenced, and the structure of the Cx45 gene was determined...
  64. ncbi Replacement by a lacZ reporter gene assigns mouse connexin36, 45 and 43 to distinct cell types in pancreatic islets
    Martin Theis
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, D 53117 Bonn, Germany
    Exp Cell Res 294:18-29. 2004
    Transcripts of three connexin isoforms (Cx36, Cx43 and Cx45) have been reported in rodent pancreatic islets, but the precise distribution of the cognate proteins is still unknown...
  65. ncbi Cardiac connexins Cx43 and Cx45: formation of diverse gap junction channels with diverse electrical properties
    Thomas Desplantez
    Physiologisches Institut, Universitat Bern, Buhlplatz 5, 3012, Bern, Switzerland
    Pflugers Arch 448:363-75. 2004
    HeLa cells expressing rat connexin43 (Cx43) and/or mouse Cx45 were studied with the dual voltage-clamp technique. Different types of cell pairs were established and their gap junction properties determined, i.e...
  66. ncbi An expression atlas of connexin genes in the mouse
    Annalisa Buniello
    Telethon Institute of Genetics and Medicine, Via P Castellino 111, 80131 Naples, Italy
    Genomics 83:812-20. 2004
    ....
  67. ncbi Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice
    Tatsuo Furuyama
    Department of Molecular Genetics and Geriatric Research, National Institute for Longevity Sciences, Kumamoto University, Kumamoto 860 0811, Japan
    J Biol Chem 279:34741-9. 2004
    ..These results suggest that Foxo1 is essential to the ability of endothelial cells to respond properly to a high dose of VEGF, thereby playing a critical role in normal vascular development...
  68. ncbi mRNA expression pattern of multiple members of connexin gene family in normal and abnormal fetal gonads in mouse
    Subhash C Juneja
    Department of Zoology, The University of Western Ontario, London, Canada
    Indian J Physiol Pharmacol 47:147-56. 2003
    ..The mRNA expression pattern of connexins (Cx26, Cx30.3, Cx31, Cx31.1, Cx32, Cx37, Cx40, Cx43, Cx45, Cx46 and Cx50) was analyzed by RT-PCR...
  69. ncbi The novel mouse connexin39 gene is expressed in developing striated muscle fibers
    Julia von Maltzahn
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, Römerstr 164, 53117 Bonn, Germany
    J Cell Sci 117:5381-92. 2004
    ..As expression of no other known connexin could be verified in these cells, intercellular dye transfer might result from functional expression of Cx39 in developing striated muscle fibers...
  70. ncbi Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43
    Roberto Araya
    Departamento de Fisiologia, Pontificia Universidad Catolica de Chile, Santiago, Chile
    J Cell Sci 118:27-37. 2005
    ..Thus, the expression of Cx45 and Cx43 is upregulated during skeletal muscle regeneration and Cx43 is required for normal myogenesis in vitro and ..
  71. ncbi Comparison of connexin 43, 40 and 45 expression patterns in the developing human and mouse hearts
    R A Kaba
    Cardiac Medicine, National Heart and Lung Institute, Imperial College, London, United Kingdom
    Cell Commun Adhes 8:339-43. 2001
    ..In general, these dynamic changes of connexins 43, 40 and 45 during mouse cardiac development appear to be mirrored in the human...
  72. ncbi Variable promoter usage and alternative splicing in five mouse connexin genes
    Curtis L Anderson
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, P O Box 016129, Miami, FL 33136, USA
    Genomics 85:238-44. 2005
    ..5'-RACE analysis of mouse embryo cDNA targeting five candidate genes, Cx31, Cx40, Cx45, Cx46, and Cx47 (approved gene symbols Gjb3, Gja5, Gja7, Gja3, and Gja12, respectively), revealed the existence of ..
  73. ncbi Expression of connexins in embryonic mouse neocortical development
    Cima Cina
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    J Comp Neurol 504:298-313. 2007
    ..and immunohistochemistry, we found that among the family of gap junction proteins, Cx26, Cx36, Cx37, Cx43, and Cx45 were expressed in the developing cortex of mice, Cx30 and Cx32 were absent, while Cx40 was expressed at a very low ..
  74. pmc Discs-large homolog 1 regulates smooth muscle orientation in the mouse ureter
    Zhen X Mahoney
    Department of Internal Medicine, Renal Division, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:19872-7. 2006
    ..Our results suggest that (i) besides its well documented role in regulating epithelial polarity, Dlgh1 also regulates smooth muscle orientation, and (ii) human DLG1 mutations may contribute to hereditary forms of hydronephrosis...
  75. ncbi Cx31 and Cx43 double-deficient mice reveal independent functions in murine placental and skin development
    Mark Kibschull
    Institute of Anatomy, University Hospital Duisburg Essen, Essen, Germany
    Dev Dyn 233:853-63. 2005
    ..Therefore, in epidermal and preimplantation development, gap junctional communication can probably be compensated by other isoforms coexpressed with Cx31 and Cx43...
  76. ncbi Different functional types of bipolar cells use different gap-junctional proteins
    Bin Lin
    Howard Hughes Medical Institute, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Neurosci 25:6696-701. 2005
    ..As reported previously (Maxeiner et al., 2005), Cx45 was found in some ON bipolar cells, but RT-PCR showed Cx36 and not Cx45 to be expressed by the type 357 bipolar ..
  77. ncbi Connexin45 interacts with zonula occludens-1 in osteoblastic cells
    J G Laing
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cell Commun Adhes 8:209-12. 2001
    Connexin43 (Cx43) and Cx45 are co-expressed in a number of different tissues...
  78. ncbi Overexpression of cardiac connexin45 increases susceptibility to ventricular tachyarrhythmias in vivo
    Tetsuo Betsuyaku
    Cardiovascular Division, Department of Medicine, Washington Univ School of Medicine, 660 South Euclid Ave, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 290:H163-71. 2006
    ..Recently, we showed that failing human hearts exhibit marked increases in connexin45 (Cx45) expression in addition to previously documented decreases in connexin43 (Cx43) expression...
  79. ncbi Dysregulation of connexins and inactivation of NFATc1 in the cardiovascular system of Nkx2-5 null mutants
    Laurent Dupays
    Laboratoire de Génétique et Physiologie du Développement UMR CNRS 6545, Institut de Biologie du Developpement de Marseille, Universite de la Mediterranee, 13288 Marseille Cedex 9, France
    J Mol Cell Cardiol 38:787-98. 2005
    ..due in part to the poor development of the ventricular trabecular network, as well as specific downregulation of Cx45 gene expression...
  80. ncbi Cardiac morphogenetic defects and conduction abnormalities in mice homozygously deficient for connexin40 and heterozygously deficient for connexin45
    Olaf Krüger
    Institute of Genetics, Division of Molecular Genetics, University of Bonn, Roemerstr 164, 53117 Bonn, Germany
    J Mol Cell Cardiol 41:787-97. 2006
    Connexin40 (Cx40) and connexin45 (Cx45) are involved in both cardiac morphogenesis and propagation of electrical activity...
  81. ncbi Localization of heterotypic gap junctions composed of connexin45 and connexin36 in the rod pathway of the mouse retina
    Karin Dedek
    Department of Neurobiology, University of Oldenburg, D 26111 Oldenburg, Germany
    Eur J Neurosci 24:1675-86. 2006
    ..Two gap junctional proteins, connexin36 (Cx36) and connexin45 (Cx45), appear to play a pivotal role in this pathway because lack of either protein leads to an impairment of visual ..
  82. ncbi Redistribution of connexin45 in gap junctions of connexin43-deficient hearts
    Carolyn M Johnson
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Cardiovasc Res 53:921-35. 2002
    Adult ventricular myocytes express two gap junction channel proteins: connexin43 (Cx43) and connexin45 (Cx45)...
  83. ncbi Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse dendritic cells
    Liliana A Corvalán
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    J Cell Physiol 211:649-60. 2007
    ..Increased levels of connexin43 and connexin45 protein and mRNA accompanied the appearance of cellular coupling. These studies provide demonstration of connexin expression and regulation by specific danger signals in DCs...
  84. ncbi Heart and head defects in mice lacking pairs of connexins
    Alexander M Simon
    Department of Physiology, University of Arizona, Tucson, AZ 85724, USA
    Dev Biol 265:369-83. 2004
    ..four are expressed in developing heart: connexin37 (Cx37), connexin40 (Cx40), connexin43 (Cx43), and connexin45 (Cx45)...
  85. pmc Melanocyte-like cells in the heart and pulmonary veins contribute to atrial arrhythmia triggers
    Mark D Levin
    Penn Cardiovascular Institute and University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 119:3420-36. 2009
    ..These data suggest that dysfunction of melanocyte-like cells in the atrium and pulmonary veins may contribute to atrial arrhythmias...
  86. ncbi Diverse functions of vertebrate gap junctions
    A M Simon
    Dept of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Trends Cell Biol 8:477-83. 1998
    ....
  87. ncbi Expression of the connexin43- and connexin45-encoding genes in the developing and mature mouse inner ear
    Martine Cohen-Salmon
    Unité de Génétique des Déficits Sensoriels, INSERM U587, Institut Pasteur, 25 rue du Dr Roux, 75724 Cedex 15, Paris, France
    Cell Tissue Res 316:15-22. 2004
    ..We took advantage of the lacZ reporter gene in Cx43 and Cx45 knock-out mice to study the expression of the connexin43 and connexin45 genes during the inner ear development...
  88. ncbi Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina
    Alexandre Hiroaki Kihara
    Department of Cell and Developmental Biology, University of Sao Paulo, Sao Paulo, SP, Brazil
    J Neurobiol 66:1397-410. 2006
    ..The present study was undertaken to determine the distribution pattern of Cx36, Cx43, and Cx45 by immunofluorescence, as well as their gene expression levels by quantitative PCR during postnatal development of ..
  89. ncbi Connexin45 (alpha 6) expression delineates an extended conduction system in the embryonic and mature rodent heart
    S R Coppen
    National Heart and Lung Institute, Imperial College School of Medicine, Royal Brompton Hospital, London, England
    Dev Genet 24:82-90. 1999
    We previously demonstrated that alpha 6 (Cx45), one of the three connexins of the mammalian myocardium, is preferentially expressed in the peripheral portion of the ventricular conduction system in rats and mice...

Research Grants1

  1. Gap Junction Mediated Signaling and Vascular Responses
    Alexander M Simon; Fiscal Year: 2010
    ..Finally, these studies could also lead to new approaches to control vasculogenesis and angiogenesis in other clinically important settings, such as anti-angiogenesis treatments designed to fight cancer. ..