Genomes and Genes
Gene Symbol: Gnas
Description: GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus
Alias: 5530400H20Rik, A930027G11Rik, C130027O20Rik, GPSA, GSP, Galphas, Gnas1, Gnasxl, Gs-alpha, Gsa, Nesp, Nesp55, Nespl, Oed-Sml, Oedsml, PHP1A, PHP1B, POH, SCG6, protein ALEX, protein GNAS, protein SCG6 (secretogranin VI), Gs alpha subunit, adenylate cyclase-stimulating G alpha protein, alternative gene product encoded by XL-exon, extra large alphas protein, guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, neuroendocrine secretory protein 55, secretogranin VI, stimulatory G-protein alpha subunit
Publications126 found, 100 shown here
- Molecular cloning and characterization of NESP55, a novel chromogranin-like precursor of a peptide with 5-HT1B receptor antagonist activityR Ischia
Department of Pharmacology, University of Innsbruck, A 6020 Innsbruck, Austria
J Biol Chem 272:11657-62. 1997..We describe here the molecular characterization of NESP55 (neuroendocrine secretory protein of Mr 55,000), a novel member of the chromogranins...
- Targeted disruption of Gnas in embryonic stem cellsW F Schwindinger
Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Endocrinology 138:4058-63. 1997..As the first step in generating a model of Albright hereditary osteodystrophy, the gene encoding G alpha(s) (Gnas) was disrupted in mouse embryonic stem (ES) cells by homologous recombination...
- Variable and tissue-specific hormone resistance in heterotrimeric Gs protein alpha-subunit (Gsalpha) knockout mice is due to tissue-specific imprinting of the gsalpha geneS Yu
Metabolic Diseases Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 95:8715-20. 1998..by short stature, obesity, and skeletal defects, is associated with heterozygous inactivating mutations of GNAS1, the gene encoding the heterotrimeric G protein alpha-subunit (Gsalpha) that couples multiple receptors to the ..
- Bidirectional imprinting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteinsB E Hayward
Human Genetics Unit, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
Proc Natl Acad Sci U S A 95:15475-80. 1998..The more 5' of these exons encodes the neuroendocrine secretory protein NESP55, which is expressed exclusively from the maternal allele...
- A cluster of oppositely imprinted transcripts at the Gnas locus in the distal imprinting region of mouse chromosome 2J Peters
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire, OX11 0RD, United Kingdom
Proc Natl Acad Sci U S A 96:3830-5. 1999..differences, to isolate candidate imprinted genes in distal Chr 2 and found two oppositely imprinted genes, Gnasxl and Nesp...
- Identification of imprinted loci by methylation-sensitive representational difference analysis: application to mouse distal chromosome 2G Kelsey
Developmental Genetics Programme, The Babraham Institute, Cambridge, United Kingdom
Genomics 62:129-38. 1999..of imprinted methylation: paternal-specific methylation at the Nesp locus and maternal-specific methylation at the Gnasxl locus...
- Paternal versus maternal transmission of a stimulatory G-protein alpha subunit knockout produces opposite effects on energy metabolismS Yu
Metabolic Diseases Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institute of Health, Bethesda, MD 20892, USA
J Clin Invest 105:615-23. 2000Heterozygous disruption of Gnas, the gene encoding the stimulatory G-protein alpha subunit (G(s)alpha), leads to distinct phenotypes depending on whether the maternal (m-/+) or paternal (+/p-) allele is disrupted...
- An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locusS F Wroe
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire, OX11 0RD, United Kingdom
Proc Natl Acad Sci U S A 97:3342-6. 2000..It contains three imprinted genes in the order Nesp-Gnasxl-Gnas...
- Identification of a methylation imprint mark within the mouse Gnas locusJ Liu
Metabolic Diseases Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 20:5808-17. 2000..is maternally expressed in some tissues and biallelically expressed in most other tissues, while the gene products NESP55 and XLalphas are maternally and paternally expressed, respectively...
- Characterization of the extra-large G protein alpha-subunit XLalphas. II. Signal transduction propertiesM Klemke
Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
J Biol Chem 275:33633-40. 2000..protein consisting of a novel 37-kDa XL domain followed by a 41-kDa alphas domain encoded by exons 2-13 of the Galphas gene. Here, we have studied the signal transduction properties of XLalphas...
- Two imprinted gene mutations: three phenotypesB M Cattanach
Medical Research Council, Mammalian Genetics Unit, Harwell, Didcot, Oxfordshire OX11 0RD, UK
Hum Mol Genet 9:2263-73. 2000..A hypothesis to explain the phenotypes associated with the three genotypes based on the NESP:/NESPAS: sense/antisense and GNASXL: transcripts in the GNAS: cluster is offered.
- Two overlapping reading frames in a single exon encode interacting proteins--a novel way of gene usageM Klemke
Department of Neurobiology, Interdisciplinary Centre of Neuroscience, University of Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
EMBO J 20:3849-60. 2001The >1 kb XL-exon of the rat XLalphas/Galphas gene encodes the 37 kDa XL-domain, the N-terminal half of the 78 kDa neuroendocrine-specific G-protein alpha-subunit XLalphas...
- Alternative non-coding splice variants of Nespas, an imprinted gene antisense to Nesp in the Gnas imprinting clusterChristine M Williamson
MRC Mammalian Genetics Unit, Harwell, Didcot, Oxfordshire, OX11 0RD, UK
Mamm Genome 13:74-9. 2002..Three of these in the order Nesp--Gnasxl--Gnas are transcribed in the sense direction with Nesp having maternal-specific expression, Gnasxl having paternal ..
- Receptor-mediated adenylyl cyclase activation through XLalpha(s), the extra-large variant of the stimulatory G protein alpha-subunitMurat Bastepe
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
Mol Endocrinol 16:1912-9. 2002XLalpha(s), the large variant of the stimulatory G protein alpha subunit (Gsalpha), is derived from GNAS1 through the use of an alternative first exon and promoter...
- The imprinted oedematous-small mutation on mouse chromosome 2 identifies new roles for Gnas and Gnasxl in developmentJudith A Skinner
Mammalian Genetics Unit, Medical Research Council, Harwell, Oxfordshire OX11 ORD, UK
Genomics 80:373-5. 2002..The maternally expressed mutant Gnas transcript is the candidate for Oed and the paternally expressed mutant Gnasxl transcript is the candidate for Sml...
- XLalphas, the extra-long form of the alpha-subunit of the Gs G protein, is significantly longer than suspected, and so is its companion AlexJoel Abramowitz
Transmembrane Signal Transduction Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
Proc Natl Acad Sci U S A 101:8366-71. 2004..XXLb proteins are likely to be found as stable dimers with AlexX. The N-terminally longer proteins may play regulatory roles...
- The imprinted signaling protein XL alpha s is required for postnatal adaptation to feedingAntonius Plagge
Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, UK
Nat Genet 36:818-26. 2004..We disrupted a paternally expressed transcript at the Gnas locus, Gnasxl, which encodes the unusual Gs alpha isoform XL alpha s...
- A cis-acting control region is required exclusively for the tissue-specific imprinting of GnasChristine M Williamson
MRC Mammalian Genetics Unit, Harwell, Oxfordshire, OX11 0RD, UK
Nat Genet 36:894-9. 2004..cluster on distal mouse chromosome 2, one at exon 1A upstream of Gnas itself and one covering the promoters for Gnasxl and the antisense Nespas (ref. 8)...
- Stimulatory G protein directly regulates hypertrophic differentiation of growth plate cartilage in vivoMurat Bastepe
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 101:14794-9. 2004..The alpha subunit of Gs (Gsalpha) is encoded by GNAS (Gnas in mice), and heterozygous Gsalpha inactivating mutations lead to Albright hereditary osteodystrophy...
- Chondrocyte-specific knockout of the G protein G(s)alpha leads to epiphyseal and growth plate abnormalities and ectopic chondrocyte formationAkio Sakamoto
Metabolic Diseases Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Bone Miner Res 20:663-71. 2005..These results show that G(s)alpha negatively regulates chondrocyte differentiation and is the critical signaling mediator of the PTH/PTH-rP receptor in growth plate chondrocytes...
- Imprinted Nesp55 influences behavioral reactivity to novel environmentsAntonius Plagge
Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
Mol Cell Biol 25:3019-26. 2005..Of these, the Nesp transcript, expressed exclusively from the maternal allele, codes for neuroendocrine secretory protein 55 (Nesp55), a chromogranin-like polypeptide associated with the constitutive secretory pathway but ..
- Alternative Gnas gene products have opposite effects on glucose and lipid metabolismMin Chen
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:7386-91. 2005..extralarge G(s)alpha (XLalphas), a paternally expressed G(s)alpha isoform; and neuroendocrine-specific protein (NESP55), a maternally expressed chromogranin-like protein...
- A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas geneEmily L Germain-Lee
Division of Pediatric Endocrinology, Department of Pediatrics, The Johns Hopkins University School of Medicine, Park Building, Suite 211, 600 North Wolfe Street, Baltimore, Maryland 21287 2520, USA
Endocrinology 146:4697-709. 2005..is caused by heterozygous inactivating mutations in GNAS, a gene that encodes not only the alpha-chain of Gs (Galphas), but also NESP55 and XLalphas through use of alternative first exons...
- Increased glucose tolerance and reduced adiposity in the absence of fasting hypoglycemia in mice with liver-specific Gs alpha deficiencyMin Chen
Metabolic Diseases Branch, NIDDK, NIH, Bethesda, Maryland 20892 1752, USA
J Clin Invest 115:3217-27. 2005..Our results define novel roles for hepatic G(s)-signaling pathways in glucose and lipid regulation, which may prove useful in designing new therapeutic targets for diabetes and obesity...
- Identification of an imprinting control region affecting the expression of all transcripts in the Gnas clusterChristine M Williamson
MRC Mammalian Genetics Unit, Harwell, Oxfordshire OX11 0RD, UK
Nat Genet 38:350-5. 2006..The group of imprinted transcripts in the mouse Gnas cluster (Nesp, Nespas, Gnasxl, Exon 1A and Gnas) provides a model for analyzing the mechanisms of imprint regulation...
- The alternative stimulatory G protein alpha-subunit XLalphas is a critical regulator of energy and glucose metabolism and sympathetic nerve activity in adult miceTao Xie
Metabolic Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:18989-99. 2006..XLalphas knock-out mice (Gnasxl(m+/p-)) have poor suckling and perinatal lethality, implicating XLalphas as critical for postnatal feeding...
- Skeletal abnormalities and extra-skeletal ossification in mice with restricted Gsalpha deletion caused by a renin promoter-Cre transgeneHayo Castrop
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Cell Tissue Res 330:487-501. 2007..Gnas encodes the alpha-subunit of the stimulatory G protein (Gs alpha)...
- Severe obesity and insulin resistance due to deletion of the maternal Gsalpha allele is reversed by paternal deletion of the Gsalpha imprint control regionTao Xie
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health, Building 10, Room 8C101, Bethesda, Maryland 20892 1752, USA
Endocrinology 149:2443-50. 2008....
- Transcription is required for establishment of germline methylation marks at imprinted genesMita Chotalia
Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB22 3AT, United Kingdom
Genes Dev 23:105-17. 2009..We show that at the Gnas locus, truncating transcripts from the furthest upstream Nesp promoter disrupts oocyte-derived methylation of the differentially methylated regions (DMRs)...
- A missense mutation in the non-neural G-protein alpha-subunit isoforms modulates susceptibility to obesityM L Kelly
MRC Harwell, Metabolism and Inflammation Section, Diabetes Group, Harwell Science and Innovation Campus, Oxfordshire, UK
Int J Obes (Lond) 33:507-18. 2009The Gnas transcription unit located within an imprinting region encodes several proteins, including the G-protein alpha-subunit, Gsalpha, its isoform XLalphas and their variant truncated neural forms GsalphaN1 and XLN1...
- Central nervous system imprinting of the G protein G(s)alpha and its role in metabolic regulationMin Chen
Signal Transduction Section, Metabolic Diseases Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell Metab 9:548-55. 2009....
- GNAS haploinsufficiency leads to subcutaneous tumor formation with collagen and elastin deposition and calcificationAkio Sakamoto
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
Endocr Res 34:1-9. 2009..We now describe the development of fibrous tumors in mice with heterozygous disruption of the Gnas gene, which encodes G(s)alpha and other gene products.
- G(s)alpha deficiency in adipose tissue leads to a lean phenotype with divergent effects on cold tolerance and diet-induced thermogenesisMin Chen
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell Metab 11:320-30. 2010..In normal mice, high-fat diet raised sympathetic nerve activity in muscle, but not in BAT. Our results show that cold- and diet-induced thermogenesis may occur in separate tissues, especially when BAT function is impaired...
- Targeted deletion of the Nesp55 DMR defines another Gnas imprinting control region and provides a mouse model of autosomal dominant PHP-IbLeopold F Fröhlich
Endocrine Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 107:9275-80. 2010..We previously identified two almost identical GNAS microdeletions extending from exon NESP55 to antisense (AS) exon 3 (delNESP55/delAS3-4)...
- Transgenic overexpression of the extra-large Gsα variant XLαs enhances Gsα-mediated responses in the mouse renal proximal tubule in vivoZun Liu
Endocrine Unit, Department of Medicine, Massachusetts General Hospital, 50 Blossom Street, Thier 10, Boston, Massachusetts 02114, USA
Endocrinology 152:1222-33. 2011..222 ± 41 pg/ml; P < 0.05). After crossing the rptXLαs mice to mice with ablation of maternal Gnas exon 1 (E1(m-/+)), male offspring carrying both the XLαs transgene and maternal Gnas exon 1 ablation (rptXLαs/E1(..
- Uncoupling antisense-mediated silencing and DNA methylation in the imprinted Gnas clusterChristine M Williamson
Medical Research Council Mammalian Genetics Unit, Harwell Science and Innovation Campus, Harwell, United Kingdom
PLoS Genet 7:e1001347. 2011..2, Nespas is a paternally expressed macroRNA that arises from an imprinting control region and runs antisense to Nesp, a paternally repressed protein coding transcript...
- Heterotopic ossifications in a mouse model of albright hereditary osteodystrophyDavid L Huso
Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
PLoS ONE 6:e21755. 2011..mediate their actions through G protein-coupled receptors, a condition termed pseudohypoparathyroidism type 1a (PHP1a)...
- Gsα enhances commitment of mesenchymal progenitors to the osteoblast lineage but restrains osteoblast differentiation in miceJOY Y WU
Endocrine Unit, Massachusetts General Hospital, 50 Blossom Street, Boston, Massachusetts 02114, USA
J Clin Invest 121:3492-504. 2011....
- Heterozygous inactivation of Gnas in adipose-derived mesenchymal progenitor cells enhances osteoblast differentiation and promotes heterotopic ossificationRobert J Pignolo
Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA
J Bone Miner Res 26:2647-55. 2011..By contrast, the GNAS proteins G(s)α, XLαs, and NESP55 are detected in adipocytes and in adipose stroma...
- Extra-long Gαs variant XLαs protein escapes activation-induced subcellular redistribution and is able to provide sustained signalingZun Liu
Endocrine Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
J Biol Chem 286:38558-69. 2011Murine models indicate that Gαs and its extra-long variant XLαs, both of which are derived from GNAS, markedly differ regarding their cellular actions, but these differences are unknown...
- Wnt/β-catenin signaling is differentially regulated by Gα proteins and contributes to fibrous dysplasiaJean B Regard
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 108:20101-6. 2011..Our data suggest that activated Gα proteins are playing physiologically significant roles during both skeletal development and disease by modulating Wnt/β-catenin signaling strength...
- New mutations at the imprinted Gnas cluster show gene dosage effects of Gsα in postnatal growth and implicate XLαs in bone and fat metabolism but not in sucklingSally A Eaton
Mammalian Genetics Unit, MRC Harwell, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom
Mol Cell Biol 32:1017-29. 2012..because suckling is unimpaired, unlike mutants in which the expression of all the known paternally expressed Gnasxl proteins (XLαs, XLN1 and ALEX) is compromised...
- Postnatal changes in the expression pattern of the imprinted signalling protein XLαs underlie the changing phenotype of deficient miceStefan O Krechowec
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
PLoS ONE 7:e29753. 2012The alternatively spliced trimeric G-protein subunit XLαs, which is involved in cAMP signalling, is encoded by the Gnasxl transcript of the imprinted Gnas locus...
- Gsα deficiency in the paraventricular nucleus of the hypothalamus partially contributes to obesity associated with Gsα mutationsMin Chen
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland 20892 1752, USA
Endocrinology 153:4256-65. 2012....
- Epigenetic properties and identification of an imprint mark in the Nesp-Gnasxl domain of the mouse Gnas imprinted locusCandice Coombes
Developmental Genetics Programme, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
Mol Cell Biol 23:5475-88. 2003..is subject to tissue-specific imprinted expression, Nesp is expressed only from the maternal allele, and Gnasxl is expressed only from the paternal allele. The mechanisms controlling these expression patterns are not known...
- Deficiency of the G-protein alpha-subunit G(s)alpha in osteoblasts leads to differential effects on trabecular and cortical boneAkio Sakamoto
Metabolic Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 280:21369-75. 2005..Osteoblast-specific G(s)alpha deficiency leads to reduced bone turnover...
- Identification of the control region for tissue-specific imprinting of the stimulatory G protein alpha-subunitJie Liu
Metabolic Diseases Branch and Genetics of Development and Disease Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:5513-8. 2005Gnas is a complex gene with multiple imprinted promoters. The upstream Nesp and Nespas/Gnasxl promoters are paternally and maternally methylated, respectively...
- Dosage-dependent switch from G protein-coupled to G protein-independent signaling by a GPCRYutong Sun
Department of Physiology, Weill Medical College, Cornell University, New York, NY 10021, USA
EMBO J 26:53-64. 2007..This new dosage-dependent switch of signaling modes of GPCRs has significant implications for GPCR intrinsic properties and desensitization...
- Gsalpha signalling suppresses PPARgamma2 generation and inhibits 3T3L1 adipogenesisLei Zhang
School of Medicine, Centre for Endocrine and Diabetes Sciences, Cardiff University, Heath Park, Cardiff, UK
J Endocrinol 202:207-15. 2009..speculated that Gbetagamma signalling may be inhibitory but failed to induce adipogenesis using activated Gsalpha (gsp*). Inhibition of phosphodiesterases did not promote adipogenesis in TSHR* or gsp* populations...
- The in vivo regulation of heart rate in the murine sinoatrial node by stimulatory and inhibitory heterotrimeric G proteinsSonia Sebastian
William Harvey Heart Centre, Barts and the London School of Medicine and Dentistry, London, United Kingdom
Am J Physiol Regul Integr Comp Physiol 305:R435-42. 2013..We conclude that Gαs- and Gαi2-mediated signaling in the sinoatrial node is important in the reciprocal regulation of heart rate through the autonomic nervous system. ..
- Agonist-independent GPCR activity regulates anterior-posterior targeting of olfactory sensory neuronsAi Nakashima
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113 0032, Japan Department of Brain Function, School of Medical Science, University of Fukui, Fukui 910 1193, Japan
Cell 154:1314-25. 2013..We conclude that the equilibrium of conformational transitions set by each OR is the major determinant of expression levels of A-P-targeting molecules...
- Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossificationJean B Regard
1 National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA 2
Nat Med 19:1505-12. 2013..In progressive osseous heteroplasia, a human disease caused by null mutations in GNAS, which encodes Gαs, Hedgehog signaling is upregulated in ectopic osteoblasts and progenitor cells...
- Loss of Gsα early in the osteoblast lineage favors adipogenic differentiation of mesenchymal progenitors and committed osteoblast precursorsPartha Sinha
Endocrine Unit, Massachusetts General Hospital, Boston, MA, USA
J Bone Miner Res 29:2414-26. 2014..These findings have clinical implications for developing therapeutic approaches to direct the commitment of mesenchymal progenitors into the osteoblast lineage...
- The G protein α subunit Gαs is a tumor suppressor in Sonic hedgehog-driven medulloblastomaXuelian He
1 Department of Forensic Medicine, State Key Laboratory of Biotherapy Collaborative Innovation Center for Biotherapy, School of Preclinical and Forensic Medicine, West China Second Hospital, Sichuan University, Chengdu, China 2 Department of Pediatrics, Brain Tumor Center, Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
Nat Med 20:1035-42. 2014..Herein, we identify GNAS, encoding the G protein Gαs, as a potent tumor suppressor gene that, when expressed at low levels, defines a ..
- Neuromodulatory effect of Gαs- or Gαq-coupled G-protein-coupled receptor on NMDA receptor selectively activates the NMDA receptor/Ca2+/calcineurin/cAMP response element-binding protein-regulated transcriptional coactivator 1 pathway to effectively induce Mamoru Fukuchi
Department of Biological Chemistry, Graduate School of Medicine and Pharmaceutical Sciences
J Neurosci 35:5606-24. 2015....
- Inactivation of a Gα(s)-PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesisRamiro Iglesias-Bartolomé
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Cell Biol 17:793-803. 2015Genomic alterations in GNAS, the gene coding for the Gαs heterotrimeric G protein, are associated with a large number of human diseases...
- Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. Evidence for a novel nucleotide exchange factorY Luo
Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 274:10685-8. 1999..These findings define a novel nucleotide exchange function for Pcp2 and suggest that the interaction between Pcp2 and Galphao is important to Purkinje cell function...
- Umami taste receptor functions as an amino acid sensor via Gαs subunit in N1E-115 neuroblastoma cellsYoshikage Muroi
Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080 8555, Japan
J Cell Biochem 113:1654-62. 2012..We concluded that Tas1r1 + 3 functions as an amino acid sensor and activates the intracellular signaling pathway through the Gαs subunit in N1E-115 cells...
- Divergent requirement for Gαs and cAMP in the differentiation and inflammatory profile of distinct mouse Th subsetsXiangli Li
Department of Medicine, UCSD, La Jolla, California 92093 0663, USA
J Clin Invest 122:963-73. 2012..we engineered mice in which the stimulatory Gα subunit of Gs (Gαs) could be deleted in T cells using CD4-Cre (Gnas(ΔCD4)). Gnas(ΔCD4) CD4(+) T cells had reduced cAMP accumulation and Ca2(+) influx...
- A mouse model for osseous heteroplasiaMichael T Cheeseman
Medical Research Council Mammalian Genetics Unit, Medical Research Council Harwell, Oxfordshire, UK
PLoS ONE 7:e51835. 2012b>GNAS/Gnas encodes G(s)α that is mainly biallelically expressed but shows imprinted expression in some tissues...
- Selective amplification and partial sequencing of cDNAs encoding G protein alpha subunits from cochlear tissuesM Tachibana
Laboratory of Molecular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892
Hear Res 62:82-8. 1992..Possible functions in the cochlea of putative G proteins with alpha-subunits partly encoded by these cDNA clones are briefly discussed and future studies are suggested...
- Mapping of the structural gene for S-adenosyl homocysteine hydrolase to mouse chromosome 2, and related sequences to chromosomes 8 and XA Pilz
Department of Genetics and Biometry, University College London, UK
Mamm Genome 3:633-6. 1992..One, Ahcy-rs1, was mapped to Chr 8 in the BXH recombinant inbred strains, and the other, Ahcy-rs2, shows a pattern of inheritance consistent with X-linkage...
- Mapping of the gene encoding the alpha subunit of the stimulatory G protein of adenylyl cyclase (GNAS1) to 20q13.2----q13.3 in human by in situ hybridizationM A Levine
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Genomics 11:478-9. 1991
- Golf: an olfactory neuron specific-G protein involved in odorant signal transductionD T Jones
Howard Hughes Medical Institute, Department of Molecular Biology and Genetic Johns Hopkins School of Medicine, Baltimore, MD 21205
Science 244:790-5. 1989..Golf alpha shares extensive amino acid identity (88 percent) with the stimulatory G protein, Gs alpha. The expression of Golf alpha in S49 cyc- kin- cells, a line deficient in endogenous stimulatory G proteins, ..
- Identification of the lesion in the stimulatory GTP-binding protein of the uncoupled S49 lymphomaT Rall
Merrell Dow Research Institute, Strasbourg, France
FEBS Lett 224:365-71. 1987..A single nucleotide transversion was found that coded for a proline rather than an arginine at residue 389. The results indicate a domain of the alpha-subunit of Gs that specifically interacts with hormone receptors...
- Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and humanC Blatt
Weizmann Institute, Rehovoth, Israel
Proc Natl Acad Sci U S A 85:7642-6. 1988..on chromosomes 3 and 1; GNAI1, GNAI2, and GNAI3 were assigned to chromosomes 7, 3, and 1, respectively; GNAZ and GNAS were found on chromosomes 22 and 20...
- Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brainH Itoh
Proc Natl Acad Sci U S A 83:3776-80. 1986..cDNAs encoding the complete sequences of Gi alpha and Gs alpha were thus obtained...
- Chromosomal localization, embryonic expression, and imprinting tests for Bmp7 on distal mouse chromosome 2P C Marker
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, California 94305 5427, USA
Genomics 28:576-80. 1995..Both embryonic expression patterns and the human chromosomal sublocalization inferred from its mouse location make Bmp7 a candidate for the gene affected in some patients with Holt-Oram syndrome...
- A high-resolution linkage map of the lethal spotting locus: a mouse model for Hirschsprung diseaseW J Pavan
Laboratory for Genetic Disease Research, National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland 20892
Mamm Genome 6:1-7. 1995..Three genes, endothelin-3 (Edn3), guanine nucleotide-binding protein alpha-stimulating polypeptide 1 (Gnas), and phosphoenolpyruvate carboxykinase (Pck1) were assessed as candidates for the ls mutation...
- Linkage mapping around the ragged (Ra) and wasted (wst) loci on distal mouse chromosome 2C Abbott
MRC Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom
Genomics 20:94-8. 1994..The results show that Ra maps very close to D2Mit74 and Acra-4, with no recombinants in 165 mice, whereas wst maps 3 cM distal to the most telomeric molecular marker on mouse chromosome 2, Acra-4...
- Mouse chromosomal location of the CCAAT/enhancer binding proteins C/EBP beta (Cebpb), C/EBP delta (Cebpd), and CRP1 (Cebpe)N A Jenkins
ABL Basic Research Program, NCI Frederick Cancer Research and Development Center, Maryland 21702 1201, USA
Genomics 28:333-6. 1995..Here, we show that Cebpb maps to mouse chromosome 2, Cebpd to chromosome 16, and Cebpe to chromosome 14. The assignment of Cebpd to chromosome 16 identifies a new region of homology between mouse chromosome 16 and human chromosome 8...
- Analysis of G protein alpha subunit mRNA abundance in preimplantation mouse embryos using a rapid, quantitative RT-PCR approachL Rambhatla
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA
Mol Reprod Dev 41:314-24. 1995..One alpha subunit mRNA increased in abundance at the early blastocyst stage. The possible significance of these alterations in G protein mRNA abundance to embryonic development is discussed...
- Phospholipid transfer protein maps to distal mouse chromosome 2R C LeBoeuf
Department of Medicine, University of Washington, Seattle, Washington, 98195, USA
Genomics 34:259-60. 1996
- Glomerular-specific imprinting of the mouse gsalpha gene: how does this relate to hormone resistance in albright hereditary osteodystrophy?C M Williamson
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire, OX11 ORD, United Kingdom
Genomics 36:280-7. 1996..Its human homologue (GNAS1) appears, from clinical and biochemical studies of patients with Albright hereditary osteodystrophy, to be ..
- The isolation and mapping of PCR markers specific to mouse Chromosome 2S Malas
Department of Genetics and Biometry, University College London, UK
Mamm Genome 7:145-8. 1996
- Normal function of the transcription factor NFAT1 in wasted mice. Chromosome localization of NFAT1 geneC Luo
Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
Gene 180:29-36. 1996..Therefore, the wasted phenotype is not due to a defect in the expression or early regulation of the NFAT1 protein...
- The laminin alpha chains: expression, developmental transitions, and chromosomal locations of alpha1-5, identification of heterotrimeric laminins 8-11, and cloning of a novel alpha3 isoformJ H Miner
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Cell Biol 137:685-701. 1997..Together, these results reveal remarkable diversity in BL composition and complexity in BL development...
- Chromosome localization of two new mammalian kinases related to yeast and fly chromosome segregation-regulatorsG Gopalan
Mammalian Genetics Laboratory, NCI Frederick Research and Development Center, Maryland 21702, USA
Mamm Genome 9:86-7. 1998
- Gs alpha knockouts in mice and manL S Weinstein
Metabolic Diseases Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Rinsho Byori 47:425-9. 1999
- BRK/Sik expression in the gastrointestinal tract and in colon tumorsX Llor
Department of Molecular Genetics, University of Illinois College of Medicine, Chicago 60607, USA
Clin Cancer Res 5:1767-77. 1999..The BRK tyrosine kinase appears to play a role in signal transduction in the normal gastrointestinal tract, and its overexpression may be linked to the development of a variety of epithelial tumors...
- BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundaryDebra E Bramblett
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Genomics 79:402-12. 2002..Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types...
- Targeted expression of activated Q227L G(alpha)(s) in vivoXi Ping Huang
Department of Molecular Pharmacology, Diabetes and Metabolic Diseases Research Program, University Medical Center, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
Am J Physiol Cell Physiol 283:C386-95. 2002....
- Erythrocyte G protein-coupled receptor signaling in malarial infectionTravis Harrison
Department of Pathology, Feinberg School of Medicine, Northwestern University, 303 Chicago Avenue, Chicago, IL 60611, USA
Science 301:1734-6. 2003..signaling via the erythrocyte beta2-adrenergic receptor and heterotrimeric guanine nucleotide-binding protein (Galphas) regulated the entry of the human malaria parasite Plasmodium falciparum...
- Identification of PSD-95 palmitoylating enzymesMasaki Fukata
Department of Physiology, University of California at San Francisco, San Francisco, California 94143, USA
Neuron 44:987-96. 2004..This study suggests that P-PATs regulate synaptic function through PSD-95 palmitoylation...
- Coding GNAS mutations leading to hormone resistance impair in vitro agonist- and cholera toxin-induced adenosine cyclic 3',5'-monophosphate formation mediated by human XLalphasAgnes Linglart
Endocrine Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, 02114, USA
Endocrinology 147:2253-62. 2006Most loss of function mutations of GNAS identified in different forms of pseudohypoparathyroidism disrupt not only the stimulatory G protein alpha-subunit (Gsalpha), but also its paternally expressed variant, XLalphas...
- Odorant receptor-derived cAMP signals direct axonal targetingTakeshi Imai
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113 0032, Japan
Science 314:657-61. 2006..Thus, it is the OR-derived cAMP signals, rather than direct action of OR molecules, that determine the target destinations of OSNs...
- YY1 as a controlling factor for the Peg3 and Gnas imprinted domainsJeong Do Kim
Department of Biological Sciences, Center for BioModular Multi Scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA
Genomics 89:262-9. 2007..also observed for the imprinted genes of the Gnas domain: up-regulation of Nespas and down-regulation of Nesp and Gnasxl. YY1 knockdown also resulted in changes in the expression levels of Xist and Snrpn...
- Chronically increased Gsalpha signaling disrupts associative and spatial learningRusiko Bourtchouladze
Center for Neurobiology and Behavior and Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
Learn Mem 13:745-52. 2006..The learning deficits observed in these transgenic mice suggest that associative and spatial learning requires regulated Gsalpha protein signaling, much as does olfactory learning in Drosophila...
- An unmethylated 3' promoter-proximal region is required for efficient transcription initiationRuth Appanah
Department of Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada
PLoS Genet 3:e27. 2007..This model provides a biochemical explanation for the typical positioning of TSSs well upstream of the 3' end of the CpG islands in which they are embedded...
- Physiological dysfunctions associated with mutations of the imprinted Gnas locusStefan Krechowec
Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, United Kingdom
Physiology (Bethesda) 23:221-9. 2008The ubiquitous Galphas-subunit of the trimeric, stimulatory G-protein plays a central role in receptor-mediated signal transduction, coupling receptor activation with the production of cAMP...
- Removal of the N-terminal extension of cardiac troponin I as a functional compensation for impaired myocardial beta-adrenergic signalingHan Zhong Feng
Section of Molecular Cardiology, Evanston Northwestern Healthcare and Northwestern University Feinberg School of Medicine, Evanston, Illinois 60201, USA
J Biol Chem 283:33384-93. 2008..These data indicate that the up-regulation of cTnI-ND may partially compensate for the cardiac inefficiency in impaired beta-adrenergic signaling...
- Chronic Galphas signaling in the striatum increases anxiety-related behaviors independent of developmental effectsChristopher Favilla
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Neurosci 28:13952-6. 2008..b>Galphas, the G-protein which stimulates adenylyl cyclase and L-type voltage-gated calcium channels, may be one ..
- Development of vascular renin expression in the kidney critically depends on the cyclic AMP pathwayBjörn Neubauer
Department of Physiology, Universitat Regensburg, Regensburg, Germany
Am J Physiol Renal Physiol 296:F1006-12. 2009....
- Haematopoietic stem cells depend on Galpha(s)-mediated signalling to engraft bone marrowGregor B Adams
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
Nature 459:103-7. 2009..Galpha(s) governs specific aspects of HSPC localization under physiological conditions in vivo and may be pharmacologically targeted to improve transplantation efficiency...
- CK2 negatively regulates Galphas signalingHeike Rebholz
Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA
Proc Natl Acad Sci U S A 106:14096-101. 2009..Thus CK2 can inhibit G protein-coupled receptor action by enabling faster receptor internalization, possibly through a direct association with Galpha(s)...
- Renal failure in mice with Gsalpha deletion in juxtaglomerular cellsLimeng Chen
National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA
Am J Nephrol 32:83-94. 2010..In the present study we have determined the long-term effect on renal function, blood pressure, and renal pathology in this low renin and diuretic mouse model...
- Thyrotrophin receptor signaling dependence of Braf-induced thyroid tumor initiation in miceAime T Franco
Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Proc Natl Acad Sci U S A 108:1615-20. 2011..Thyroid-specific deletion of the Gsα gene in LSL-Braf(V600E)/TPO-Cre/Gnas-E1(fl/fl) mice also resulted in an attenuated cancer phenotype, indicating that the cooperation of TshR with ..
- Absence of the glucagon-like peptide-1 receptor does not affect the metabolic phenotype of mice with liver-specific G(s)α deficiencyMin Chen
Metabolic Diseases Branch, Building 10 Room 8C101, National Institute for Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1752, USA
Endocrinology 152:3343-50. 2011....
- Diversity of the G-protein family: sequences from five additional alpha subunits in the mouseM Strathmann
Division of Biology, California Institute of Technology, Pasadena 91125
Proc Natl Acad Sci U S A 86:7407-9. 1989..In addition, we found sequences corresponding to five newly discovered alpha subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected...
- Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the alpha chainsK A Sullivan
Proc Natl Acad Sci U S A 83:6687-91. 1986..Further comparison of the alpha-chain sequences suggests additional regions that may contribute to interactions with beta gamma subunits and the receptor and effector components of different signal transduction systems...
- 71.67 T lymphocyte activation is accompanied by the down-regulation of three G alpha genesB Rock
Department of Dermatology, Emory University, Atlanta, Georgia 30322, USA
Cell Immunol 164:316-21. 1995..These findings suggest that an important element in signal transduction through these G proteins in lymphocytes may include regulation of G alpha mRNA steady-state levels...
- Circulating osteogenic cells in aging and diseaseRobert Pignolo; Fiscal Year: 2007..LONG-TERM OBJECTIVES: To define the role of BdACs as osteogenic cells with potential for use in diagnostic testing, cell replacement therapy, or as a target population for gene therapy. ..
- BHLH FACTOR IN DIABETES & PANCREAS DEVELOPMENTDEBRA BRAMBLETT; Fiscal Year: 2003..The BETA4 knockout will be a mouse model system for the study of aspects of pancreas development and possibly pancreatic disease. ..
- Regulation of Meiotic Arrest in Oocytes by G-ProteinsLISA MEHLMANN; Fiscal Year: 2004..Understanding how the oocyte cell cycle is regulated within the follicle may find applications to practical problems including assisted reproductive techniques, contraception, and infertility. ..